scholarly journals First report from Bangladesh on genetic diversity of multidrug-resistant Pasteurella multocida type B:2 in fowl cholera

2021 ◽  
pp. 2527-2542
Author(s):  
Otun Saha ◽  
M. Rafiul Islam ◽  
M. Shaminur Rahman ◽  
M. Nazmul Hoque ◽  
M. Anwar Hossain ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Pasteurella Multocida ◽  
Laying Hens ◽  
Multidrug Resistant ◽  
Bacterial Disease ◽  
Poultry Production ◽  
Type B ◽  
Fowl Cholera ◽  
Factors Associated ◽  
Pathogenic Genes

Background and Aim: Fowl cholera (FC) caused by Pasteurella multocida is a highly contagious bacterial disease of global importance for poultry production. The severity and incidence of FC caused by P. multocida may vary considerably depending on several factors associated with the host (including species and age of infected birds), the environment, and the bacterial strain. This study aimed to investigate the genetic diversity of multidrug-resistant P. multocida strains isolated from FC outbreaks in laying hens from commercial farms of Bangladesh. Materials and Methods: We collected 57 samples of suspected FC, including 36 live and 21 dead laying hens. P. multocida isolates were characterized by biochemical and molecular-biological methods. Results: Twenty-two strains of P. multocida were isolated from these samples through phenotypic and genotypic characterization. The strains were grouped into two distinct random amplification of polymorphic DNA (RAPD) biotypes harboring a range of pathogenic genes; exbB, ompH, ptfA, nanB, sodC, and hgbA. In this study, 90.90% and 81.82% P. multocida strains were multidrug-resistant and biofilm formers, respectively. Whole-genome sequencing of the two representative RAPD phylotypes confirmed as P. multocida type B: L2:ST122, harboring a number of virulence factors-associated genes (VFGs), and antimicrobial resistance (AMR) genes (ARGs). In addition, pan-genome analysis revealed 90 unique genes in the genomes of P. multocida predicted to be associated with versatile metabolic functions, pathogenicity, virulence, and AMR. Conclusion: This is first-ever report on the association of P. multocida genotype B: L2:ST122 and related VFGs and ARGs in the pathogenesis of FC in laying hens. This study also provides a genetic context for future researches on the evolutionary diversity of P. multocida strains and their host adaptation.

scholarly journals Genome-wide diversity and differentiation of two novel multidrug-resistant populations of Pasteurella multocida type B:2 from fowl cholera

2020 ◽  
Author(s):  
Otun Saha ◽  
M. Rafiul Islam ◽  
M. Shaminur Rahman ◽  
M. Nazmul Hoque ◽  
M. Anwar Hossain ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Pasteurella Multocida ◽  
Severe Disease ◽  
Multidrug Resistant ◽  
Etiologic Agent ◽  
Mortality And Morbidity ◽  
Fowl Cholera ◽  
Metabolic Functions ◽  
Pathogenic Genes ◽  
Antimicrobial Resistance Genes

ABSTRACTPasteurella multocida is the etiologic agent of fowl cholera (FC), a highly contagious and severe disease in poultry with higher mortality and morbidity. Twenty-two P. multocida strains isolated from the FC outbreaks were subjected to phenotypic and genotypic characterization. The isolates were grouped into two distinct RAPD biotypes harboring a range of pathogenic genes; exbB, ompH, ptfA, nanB, sodC, and hgbA. Among these strains, 90.90% and 36.37% were multidrug-resistant and strong biofilm formers, respectively. Whole genome sequencing of the two representative RAPD isolates confirmed as P. multocida type B:L2:ST122 harboring a number of virulence factors, and antimicrobial resistance genes. Pan-genome analysis revealed 90 unique genes in these genomes associated with versatile metabolic functions, pathogenicity, virulence, and antimicrobial resistance. This study for the first time reports the association of P. multocida genotype B:L2:ST122 in the pathogenesis of FC, and provides a genetic context for future researches on P. multocida strains.

scholarly journals Cholera Case Study on Broiler Poultry Collected from Communities Farm in Banda Aceh Using Pathology Method

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Zainuddin Z
Keyword(s):  
Broiler Chickens ◽  
Pasteurella Multocida ◽  
Mortality Rates ◽  
Morbidity And Mortality ◽  
Bacterial Disease ◽  
High Morbidity ◽  
Northern Iran ◽  
Fowl Cholera ◽  
The World

Fowl cholera is an infectious bacterial disease that is widespread in poultry in the world caused by Pasteurellamultocida. The disease infects both fowl and wild poultry. Poultry become more sensitive to the disease on advance age of chicken and on laying chicken. Generally, thisdisease infects breeding flocks of males with the aged of 33 weeks, and in breeding flocks at age 35 weeks with the symptoms of increasedmortality, and wattle and leg swelling. P multocida infection in chickens aged 25 weeks was reported in Korea. In northern Iran, cholera hasbeen reported endemic in poultry breeders. In chickens, this disease can be manifested in the acute and chronic forms. Generally, fowl cholera isasepticemic disease characterized by high morbidity and mortality rates, usually runs an acute, but in an endemic area the poultry is lesssensitive to this disease, thus,it was grouped to chronic disease. Chronic form of fowl cholera may occur after the outbreak. Recently, fowlcholera incidence in broiler chickens is becoming more common. These cases are associated with farm conditions that are very unhygienic andthe contact between chickens and carrier animals. In Indonesia P. multocida has been isolated from sudden deathlaying hens and broilers. InAceh province incidence of cholera in poultry broiler farms and chicken is quite high, the rate of morbidity and mortality are high.____________________________________________________________________________________________________________________Key words: chicken, fowl cholera, Pasteurella multocida

scholarly journals High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shahana Ahmed ◽  
Tridip Das ◽  
Md Zohorul Islam ◽  
Ana Herrero-Fresno ◽  
Paritosh Kumar Biswas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Broiler Chickens ◽  
Multidrug Resistant ◽  
Genomic Diversity ◽  
Poultry Production ◽  
E Coli ◽  
Strain Collection ◽  
Antimicrobial Resistance Genes ◽  
High Prevalence

Abstract Colistin is a last-resort antimicrobial used for the treatment of human infections caused by multidrug-resistant Gram-negative bacteria. However, colistin is still widely used in intensive poultry production in Bangladesh. We aimed to investigate the dynamics and genetic diversity of colistin-resistant commensal Escherichia coli from broiler chickens. A total of 1200 E. coli strains were characterized from 20 broiler farms at three-time points along the production period. All strains were screened for mcr-1 to mcr-5 genes by a multiplex PCR, and their genetic diversity was measured by repetitive extragenic palindromic (REP)-PCR fingerprinting. Genomic diversity and characterization were performed by whole genome sequencing (WGS). Twenty-five percent of the commensal E. coli strains harbored mcr-1 genes. Frequency of mcr-1 gene detection correlated positively (odds ratio 1.71; 95% CI 0.96–3.06; p = 0.068) with the use of colistin in poultry flocks. REP-PCR profiles and WGS analysis showed diverse E. coli population carrying multiple antimicrobial resistance genes. Phylogenetic comparison of mcr-1-bearing strains recovered from this study with a global strain collection revealed wide phylogenetic relationship. This study identified a high prevalence of mcr-1 gene among genetically diverse E. coli populations from broiler chickens in Bangladesh suggesting a massive horizontal spread of mcr-1 rather than by clonal expansion.

scholarly journals Molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Hamadan, west of Iran

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Maryam Hazhirkamal ◽  
Omid Zarei ◽  
Mahsa Movahedi ◽  
Pezhman Karami ◽  
Leili Shokoohizadeh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Acinetobacter Baumannii ◽  
Nosocomial Infections ◽  
Molecular Typing ◽  
Biofilm Formation ◽  
Opportunistic Pathogen ◽  
Multidrug Resistant ◽  
Factors Associated ◽  
Biofilm Production ◽  
Resistance Patterns

Abstract Background Acinetobacter baumannii is an opportunistic pathogen that can cause several kinds of nosocomial infections. Increasing antibiotic resistance as well as identifying genetic diversity and factors associated with pathogenicity and prevalence of this bacterium is important. The aim of this study was the investigation of molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Iran. Methods Forty isolates of A. baumannii were obtained from various wards of the central hospital, in the west of Iran. Phenotypic identification and genetic diversity, biofilm production assay, and detection of Carbapenemase genes carried out. Results Tracheal samples 26 (61.9 %) are the most frequent isolates, and 95 % of isolates were identified as MDR. 32.5 % of all A. baumannii strains were capable to form a strong biofilm. It was founded that antimicrobial resistance patterns had a significant relationship with strong biofilm formation (P = 0.001). Most frequencies of the studied genes were in the order of VIM (81 %), SPM (45.2 %), and IMP (35.7 %) genes. The VIM gene was the most frequent in all isolates which were significant (P = 0.006). 14 different ERIC-types were observed including 7 common types and 7 unique or single types. F type is the largest common type consisting of nine isolates and B, D, and E types contain two isolates separately. Conclusions ERIC-PCR technique was used to genetically classify A. baumannii isolates as one of the most common microorganisms in nosocomial infections.

Multidrug Resistant Pasteurella multocida Strains Isolated from Chickens with Cases of Fowl Cholera in Jos, Nigeria

2013 ◽  
Vol 12 (10) ◽  
pp. 596-600 ◽  
Author(s):  
Y.D. Dashe ◽  
M.A. Raji ◽  
P.A. Abdu ◽  
B.S. Oladele ◽  
M.Y. Sugun
Keyword(s):  
Pasteurella Multocida ◽  
Multidrug Resistant ◽  
Fowl Cholera

Immunogenicity and Protective Efficacy of Iron-inactivated Pasteurella multocida A:1 Vaccine against Fowl Cholera in Backyard Chicken

2021 ◽  
Author(s):  
M. Abhilash ◽  
T.R. Kannaki ◽  
E. Priyanka ◽  
Santosh Haunshi
Keyword(s):  
Pasteurella Multocida ◽  
Protective Efficacy ◽  
Economic Loss ◽  
Bacterial Disease ◽  
Oil Emulsion ◽  
Fowl Cholera ◽  
Antibody Titers ◽  
Mortality Percentage ◽  
Backyard Chicken ◽  
Significant Economic Loss

Background: Fowl cholera is a highly fatal, contagious bacterial disease that incurs significant economic loss in commercial as well as back-yard poultry. Vaccination is the most effective way in controlling this disease. In this study, we prepared and evaluated the immunogenicity of iron- inactivated Pasteurella multocida A:1 vaccine and its protective efficacy against fowl cholera experimental infection in backyard chicken.Methods: Scaled up Pasteurella multocida A:1 culture with 5 X 108 CFU/ml equivalent to 2.5 mg of antigen per dose was used for preparation of experimental vaccines. Formalin inactivated and mixed with APS adjuvant (FIA), formalin inactivated-Freund adjuvant (FIF), Iron inactivated and adjuvanted with iron (III), Iron inactivated from iron supplemented media and adjuvanted with iron (ISII) and commercial oil emulsion vaccine (CV) were used in the study. A total of 120 Vanaraja birds (n=20/group) of 2 weeks age were immunized with these vaccine and booster were given at 3rd and 6th week with respective vaccine. Specific antibody titers were assessed by iELISA in the serum at weekly intervals. The birds were challenged (n=6 /group) with 5x104 CFU/ml of virulent isolate by intraperitoneal route and morbidity, mortality percentage were observed.Result: Protective antibody titers were induced by iron inactivated vaccine from 4th week of immunization and upon booster doses it induced significantly higher (P less than 0.05) antibody response. The iron inactivated experimental vaccine gave equivalent protection as that of commercial vaccine upon challenge infection.

NEW GENERATION FEED ADDITIVE IN POULTRY BREEDING

1970 ◽  
pp. 67-69
Author(s):  
L. I. Naumova ◽  
М. Т. Klychnikov ◽  
N. F. Klychnikova
Keyword(s):  
Laying Hens ◽  
Far East ◽  
Brown Seaweed ◽  
Test Group ◽  
Live Weight ◽  
Production Quality ◽  
Feed Additive ◽  
Poultry Production ◽  
Omega 3 ◽  
Mineral Substances

The analysis of different doses of complex feed additive to poultry ration have been made. The influence of new feed additive with bioresources of the Far East use on productive poultry indexes, on production quality and digestibility of nutritious matters of mixed feed has been studied. Patrinia scabiosifolia, Acanthopanax sesseliflorum, cones of cedare nut husk, brown seaweed, macro- and microelements, vitamins were the object of research. As a result of these researches author presents more effective norms of complex feed additive use in poultry farming of Khabarovsk Kray. The recipe of feed additive from local vegetable resources and complex of vitamins and minerals for use in laying hens nutrition was worked out. The aim of this work was to rise zootechnic and economic indexes of poultry production. The introduction of complex feed additive in ration influenced positively on the digestibility, raised the content of Omega-3 fat acid in egg of laying hens. The increase of Omega-3 fat acid quantity promotes decrease of cholesterol in yolk of egg. In the experimental groups there was a more intensive use of nutrients in diet, mainly due to raw protein, fats, mineral substances – calcium and phosphorus. The use of a new feed additive had a positive effect on the hatchability of eggs, the development of internal organs (heart, liver) and the live weight of chicks obtained from the eggs of chickens in experimental groups. Additionally, the content of mineral substances (calcium, potassium, phosphorus) in the egg of test group was higher than in the control on average by 2.3-5.1%.

scholarly journals Temporal Trend of ST131 Clone among Urinary Escherichia coli Isolates in the Community: A Taiwan National Surveillance from 2002 to 2016

2021 ◽  
Vol 9 (5) ◽  
pp. 963
Author(s):  
Jiun-Ling Wang ◽  
Wen-Chien Ko ◽  
Chih-Hsin Hung ◽  
Ming-Fang Cheng ◽  
Hui-Ying Wang ◽  
...  
Keyword(s):  
Temporal Trend ◽  
Multidrug Resistant ◽  
Population Based ◽  
Pulsed Field Gel Electrophoresis ◽  
Sequence Type ◽  
E Coli ◽  
Factors Associated ◽  
Resistant Group ◽  
Ciprofloxacin Resistance ◽  
Susceptible Group

Sequence type (ST) 131 is a multidrug-resistant pandemic lineage of E. coli responsible for extraintestinal infections. Few surveillance data of ST131 included all antimicrobial-susceptible and -resistant isolates or focused on community-acquired urinary tract infection (UTI). From a population-based surveillance pool of 2997 outpatient urine E. coli isolates, 542 were selected for detection of ST131 based on ciprofloxacin and/or cefotaxime resistance. Pulsed-field gel electrophoresis (PFGE) was performed on all ST131 isolates to further determine their relatedness. The estimated overall ST131 prevalence in this community UTI cohort increased from 11.2% (in 2002–2004), 12.2% (in 2006–2008), 13.6% (in 2010–2012), to 17.4% in 2014–2016 (p < 0.01). In the ciprofloxacin-resistant/cefotaxime-resistant group, ST131 increased from 33.3% in 2002–2004 to 72.1% in 2014–2016 (p < 0.01). In the ciprofloxacin-resistant/cefotaxime-susceptible group, ST131 was found in 24.3% overall without significant increase in its prevalence over time. PFGE showed emergence of a cluster of ciprofloxacin-resistant/cefotaxime-resistant ST131 carrying Gr. 1 CTX-M ESBL in 2014–2016, especially 2016. Multivariate analysis revealed that age (≥65 y.o) and ciprofloxacin resistance were independent factors associated with ST131. This longitudinal surveillance showed that ciprofloxacin-resistant/cefotaxime-susceptible ST131 has been circulating in the community since 2002 but ciprofloxacin-resistant/cefotaxime-resistant ST131 increased rapidly in the later years.

scholarly journals Novel multi-strain probiotics reduces Pasteurella multocida induced fowl cholera mortality in broilers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rine Christopher Reuben ◽  
Shovon Lal Sarkar ◽  
Habiba Ibnat ◽  
Md. Ali Ahasan Setu ◽  
Pravas Chandra Roy ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Efficiency ◽  
Biochemical Parameters ◽  
Pasteurella Multocida ◽  
White Blood Cells ◽  
Clinical Signs ◽  
Basal Diet ◽  
Prostaglandin E ◽  
Fowl Cholera ◽  
Anti Inflammatory

AbstractPasteurella multocida causes fowl cholera, a highly contagious poultry disease of global concern, causing significant ecological and economic challenges to the poultry industry each year. This study evaluated the effects of novel multi-strain probiotics consisting of Lactobacillus plantarum, L. fermentum, Pediococcus acidilactici, Enterococcus faecium and Saccharomyces cerevisiae on growth performance, intestinal microbiota, haemato-biochemical parameters and anti-inflammatory properties on broilers experimentally challenged with P. multocida. A total of 120 birds were fed with a basal diet supplemented with probiotics (108 CFU/kg) and then orally challenged with 108 CFU/mL of P. multocida. Probiotics supplementation significantly (P < 0.05) improved growth performance and feed efficiency as well as reducing (P < 0.05) the population of intestinal P. multocida, enterobacteria, and mortality. Haemato-biochemical parameters including total cholesterol, white blood cells (WBC), proteins, glucose, packed cell volume (PCV) and lymphocytes improved (P < 0.05) among probiotic fed birds when compared with the controls. Transcriptional profiles of anti-inflammatory genes including hypoxia inducible factor 1 alpha (HIF1A), tumor necrosis factor- (TNF) stimulated gene-6 (TSG-6) and prostaglandin E receptor 2 (PTGER2) in the intestinal mucosa were upregulated (P < 0.05) in probiotics fed birds. The dietary inclusion of the novel multi-strain probiotics improves growth performance, feed efficiency and intestinal health while attenuating inflammatory reaction, clinical signs and mortality associated with P. multocida infection in broilers.

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