scholarly journals QUANTITATIVE CONTENT OF THE MAIN ACTIVE SUBSTANCES IN THE ROOTS OF THE NATURAL AND IN-TRODUCED PLANT SAPOSHNIKOVIA DIVARICATE (TURCZ.) SCHISCHK.

2021 ◽  
pp. 143-151
Author(s):  
Bayarma Munhoevna Urbagarova ◽  
Vasiliy Vladimirovich Taraskin ◽  
Tat'yana Valer'yevna Elisafenko ◽  
El'vira Eduardovna Shults ◽  
Elena Anatol'yevna Korolyuk ◽  
...  
Keyword(s):  
Raw Materials ◽  
Natural Populations ◽  
Total Content ◽  
Climatic Conditions ◽  
Raw Material ◽  
First Year ◽  
Quantitative Content ◽  
Two Samples ◽  
Saposhnikovia Divaricata ◽  
Active Substances

A HPLC-UV method was developed for the quantitative analysis of chromones in the roots of Saposhnikovia divaricata (Turcz.) Schischk. The optimal parameters of chromones extraction (two-fold ultrasonic extraction with a duration of 40 min with 50% ethyl alcohol at a raw material-extractant ratio of 1:10) were selected. The validation analysis showed that the proposed method is characterized by satisfactory metrological parameters. The method was used for the analysis of raw materials of wild, introduced and commercial samples of S. divaricata. In this work, for the first time in the roots of S. divaricata from natural populations of Buryatia, Trans-Baikal Territory, Mongolia and in the introduced samples, the HPLC-UV method was used to determine the quantitative content of the main active substances: prim-O-glucosylcimifugine (0.13–5.22 mg/g), cimifugine (0.01– 1.82 mg/g) and 4’-O-β-D-glucosyl-5-O-methylvisamminol (0.98–3.25 mg/g). The total content of chromones varies depending on the place of collection and the phase of vegetation. The content of chromones in two samples of roots from introduced S. divaricatae, collected in the first year of introduction in different climatic conditions, was established.

scholarly journals HPLC determination of phenolic compounds content in Parmelia sulcata and Parmelia vagans thalli

Pharmacia ◽  
2019 ◽  
Vol 66 (4) ◽  
pp. 161-164
Author(s):  
O. A. Kyslychenko ◽  
Viktoriia V. Protska ◽  
Iryna O. Zhuravel
Keyword(s):  
Phenolic Compounds ◽  
Inflammatory Diseases ◽  
Raw Materials ◽  
Folk Medicine ◽  
Usnic Acid ◽  
Total Content ◽  
Raw Material ◽  
Parmelia Sulcata ◽  
Quantitative Content

The species of Parmelia genus have long been used in Indian folk medicine for the treatment of bronchitis, ulcers, furunculosis, cardiovascular diseases, urolithiasis, amenorrhea, and also at infectious and inflammatory diseases. In Ukraine, the most common lichens of the Parmelia genus are Parmelia sulcata Tailor and Parmelia vagans Nyl. At the same time, thalli of Parmelia genus lichens belong to the non-officinal and poorly studied types of raw material. The qualitative composition and the quantitative content of phenolic compounds in Parmelia sulcata and Parmelia vagans thalli was studied by HPLC. According to the results of the chromatographic analysis, salazinic, fumaroprotocetraric, usnic acids, chloratranorin and atranorin were identified in both types of raw material studied. In addition, protocetraric acid was identified in Parmelia sulcata thalli. According to the results of the experiment, the total content of identified phenolic compounds in Parmelia sulcata thalli was 2019.71±40.39 g/mol, and in Parmelia vagans thalli it comprised 1754.18±34.77 g/mol. In the thalli of both studied species of Parmelia genus, fumaroprotocetraric acid dominanted by the quantity. This substance was present in Parmelia sulcata thalli in the amount of 474.00±9.00 g/mol, and in Parmelia vagans thalli – 456.21±8.67 g/mol. In addition, a significant amount of chloratranorin (408.79±8.99 g/mol) was present in Parmelia sulcata thalli. Quite a high content of atranorin (393.34±8.65 g/mol) and usnic acid (375.31±7.53 g/mol) were defined in Parmelia vagans thalli. The results obtained can be used in the development of quality control methods for Parmelia sulcata and Parmelia vagans thalli, as well as medicines based on these types of raw materials.

scholarly journals Quantitative content of flavonoids in plant samples from the Crimean population of oregano (Origanum vulgare)

2021 ◽  
Vol 39 ◽  
pp. 02006
Author(s):  
A.V. Mishnev ◽  
E.F. Myagkikh ◽  
I.V. Belova
Keyword(s):  
Raw Materials ◽  
Natural Habitat ◽  
Total Content ◽  
Raw Material ◽  
Indigenous Populations ◽  
Origanum Vulgare ◽  
Crimean Peninsula ◽  
Average Value ◽  
Quantitative Content ◽  
Total Flavonoids Content

The aim of the research was to analyze the quantitative content of flavonoids in raw oregano (Origanum vulgare L.) from indigenous flora from different geographical locations of the Crimean peninsula. The objectives of the research included: the selection of promising samples for breeding, conclusion on the potential use of raw materials collected from regional indigenous populations for medical purposes. It was found that the raw oregano material in most cases did not conform to the requirements of the pharmacopoeial monograph RF XIV in terms of the total flavonoids content equivalent to luteolin and can not be recommended for use as an official raw material. The total content of flavonoids in herbal raw materials varied from 0.40 to 1.05% with an average value of 0.69%. Only three of the tested samples exceeded the threshold of 0.80%, with 0.81–1.05% value, one sample had this index at the level of the lower threshold, 0.80%. No high-flavonoid samples that would be valuable as donors of this trait for breeding varieties of medicinal direction have been identified. The conclusion was made that it is advisable to grow high-flavonoid varieties specially created for this purpose instead of collecting oregano herbal materials in their natural habitat.

LEVISTICUM OFFICINALE (F. APIACEAE): HABITAT, BOTANICAL DESCRIPTION AND CHEMICAL COMPOSITION (REVIEW)

2021 ◽  
pp. 67-75
Author(s):  
Kubasova E.D. ◽  
Korelskaya G.V. ◽  
Kubasov R.V.
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Raw Materials ◽  
Climatic Conditions ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Universal System ◽  
Plant Raw Materials ◽  
Active Substances

The Medicinal Lovage (Levisticum Officinale) grows almost everywhere. In order to use Levisticum Officinale in economic needs, including in medicine, a number of countries are specially engaged in its cultivation. The analysis of the scientific literature revealed a large number of publications confirming the diverse chemical spectrum of rhizomes, roots and herbs of the medicinal lovage. In this regard, it is used as a medicinal raw material and is included in a number of European Pharmacopoeias. For medical purposes, all parts of this plant that contain biologically active substances can be used. Levisticum officinale contains various phytochemical compounds and secondary metabolites – terpenoids, flavonoids, essential oils, phthalides, polyacetylenes, coumarins, phenylpropanoids, alkaloids, tannins, phenolic acids, polyacetylenes. All of them have properties that can have a therapeutic effect on the organism. The composition of essential oils of Levisticum Officinale is the most studied. It is mainly represented by monoterpene hydrocarbons, sexwiterpenes and phthalides. There are some differences in the chemical composition of Levisticum Officinale. Most scientists agree that such a discrepancy may be related to the period and process of harvesting plant raw materials, age, genotype of the plant, climatic conditions and place of growth, as well as the method of extraction and the method of identification and quantitative determination of biologically active substances. Therefore, there is a problem of a single universal system of preparation and further use of raw materials. The development of modern methods for analyzing the raw materials of lovage and the study of its chemical composition creates prerequisites for expanding its use as a medicinal plant.

scholarly journals BIOLOGICALLY ACTIVE SUBSTANCES OF THE HERB OF URTICA DIOICA

2006 ◽  
Vol 14 (1) ◽  
pp. 25
Author(s):  
V. Ya. Yatziuk ◽  
G. A. Chaly ◽  
O. V. Soshnikova
Keyword(s):  
Total Content ◽  
Urtica Dioica ◽  
Water Soluble ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Quantitative Content ◽  
Phenol Composition ◽  
First Time ◽  
Active Substances

We have established the qualitative composition and the quantitative content of biologically active substances of the herb of Urtica dioica. Aminoacid composition, monosaccharide set of PSK and PV, the presence of 4 macro- and 18 microelements and their quantitative content have been studied for the first time. Investigating the phenol composition of the herb of Urtica dioica by HPLC has revealed in the raw material alongside with the previously described gallic, coffeic acids, rutine and quercetine the presence of hyperoside, luteolin-7-glycoside, 3,4-isoramnetin diglycoside, chlorogenic acid, aesculetin. We were the first to determine the total content of water-soluble polysaccharides, pectin substances, astringent substances, pigments (carotenoids and chlorophylls),vitamin K1, triterpene compounds and saponins of the triterpene type.

scholarly journals Phenolic Compounds and Antioxidant Potential of Wild-Growing Plant Materials of the North Caucasus Region

2019 ◽  
Vol 9 (2) ◽  
pp. 5062-5071 ◽  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Raw Materials ◽  
Functional Materials ◽  
Total Content ◽  
Raw Material ◽  
North Caucasus ◽  
Plant Materials ◽  
The North ◽  
Quantitative Content

The article presents the results of studying the qualitative composition and quantitative content of some groups of phenolic compounds in 11 types of medicinal plants growing in the foothills of the North Caucasus, and provides the estimates of the antioxidant activity of extracts from these plants. The qualitative and quantitative content of phenolic compounds was determined using a Kapel-105M capillary electrophoresis system, and the total antioxidant activity of the extracts was measured on a Tsvet Yauza-01-AA device with an amperometric detector. In the studied plant samples, the total content of tannins was determined, eight phenolcarbonic acids were identified and quantified, as well as quercetin and rutin — two of the most important flavonols. The highest total content of phenolcarbonic acids (11,776.2 mg/kg), as well as the highest antioxidant activity were noted in the aqueous extract obtained from Echinacea purpurea (lat. Echinacea angustifolia). The direct relationship between the antioxidant activity of the studied medicinal raw material and the content of phenolic compounds has been experimentally established as follows: the higher is the concentration of phenolic substances, the higher is the antioxidant activity. The results of this study provide new information on the composition and content of phenolic compounds in some types of wild-growing plant raw materials of the North Caucasus and the antioxidant activity of extracts based thereon that will facilitate the use of the studied plants as a potential source of natural antioxidants in the production of functional materials.

scholarly journals HPLC study of phenolic compounds in Mirabilis jalapa raw material

Pharmacia ◽  
2020 ◽  
Vol 67 (3) ◽  
pp. 145-152
Author(s):  
Dhurgham Khalid Abed Sarray ◽  
Liliia M. Horiacha ◽  
Iryna O. Zhuravel ◽  
Andrii I. Fedosov
Keyword(s):  
Phenolic Compounds ◽  
Folk Medicine ◽  
Total Content ◽  
Hplc Method ◽  
Raw Material ◽  
Control Methods ◽  
Quantitative Content ◽  
Different Types ◽  
Mirabilis Jalapa ◽  
Hplc Study

Mirabilis jalapa is a popular decorative plant valued for its beautiful multicolored flowers. Folk medicine in various countries applies Mirabilis jalapa as anti-microbial, anti-inflammatory, diuretic, spasmolytic drug. Chemical composition of different types of Mirabilis jalapa has not yet been adequately studied which is an obstacle for its application in medicine. The qualitative composition and quantitative content of phenolic compounds were studied by the HPLC method. The performed experiment revealed presence of hydroxycinnamic acids, flavonoids, isoflavonoids and coumarins in tested herb. The content of phenolic compounds was the highest in Mirabilis jalapa flowers, counting as much as 2977.41 ± 59.55 µg/mg. Total content of phenolic compounds in Mirabilis jalapa herb was 304.25 ± 6.08 µg/mg, in fruits – 67.92 ± 1.36 µg/mg, and in roots – 12.44 ± 0.25 µg/mg. Quantitatively neochlorogenic acid dominated in flowers, chlorogenic acid in fruits, whereas Mirabilis jalapa herb mostly contained rutin and hyperoside. The obtained results will be useful in the development of quality control methods for Mirabilis jalapa herb and manufacture of drug preparations on its basis.

scholarly journals Scientific substantiation and the release of saponins from plant raw material for food and cosmetic cream technology

2018 ◽  
Vol 7 (2.13) ◽  
pp. 297
Author(s):  
Svetlana N. Butova ◽  
Vera A. Salnikova ◽  
Lyudmila A. Ivanova ◽  
Irina D. Schegoleva ◽  
Lyudmila A. Churmasova
Keyword(s):  
Raw Materials ◽  
Chemical Properties ◽  
Scientific Work ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
State University ◽  
Secondary Products ◽  
Vegetable Raw Materials ◽  
Active Substances

The article presents the results of author scientific research dealing with the use of saponins, biologically active substances in food and cosmetics technology. The problems of their formation in plants, the chemical nature and the features are considered, biological and physico-chemical properties of saponins are studied. By their nature, saponins are divided into steroid and triterpene, differing by glycoside part of a molecule, thus, with different biological and chemical properties, but they are all capable to develop foam in aqueous solutions, and this feature is the origin of their name. The name originated from the word «Sapo», which means soap. It should be noted that at present saponins are not studied fully as other biologically active substances (BAS), although they are of interest and relevance. In the course of scientific work, about 20 species of saponin-containing plant material, both steroid and triterpene ones, were selected and analyzed for the determination of saponins. The selection of raw materials with the highest content was performed for their further use in cosmetics and as the surfactants in the production of emulsion food products. The following research methods were used in the work: qualitative reactions to the presence of saponins, foaming, saponin extraction, the release from dry aqueous extract of both steroid and triterpin ones. The method and the scheme of their release and precipitation are presented in the article.The work was carried out within the framework of the state assignment of FSBEI HE «Moscow State University of Food Production» No. 14.7404.2017/бч «Scientific and applied bases of application of traditional and nonconventional vegetable raw materials and secondary products of its processing (fruit and berry, grain, bean, oil, essential oil, herbs) in technology of specialized products of the food industry, cosmetology and pharmacy»  

scholarly journals Research in the Development and Validation of the Method of Quantitative Determination of Flavonoids in the Common Sorrel Herba, Gathered in the Altai Territory

2019 ◽  
Vol 8 (2) ◽  
pp. 80-86 ◽  
Author(s):  
G. R. Kutateladze ◽  
L. M. Fedoseeva
Keyword(s):  
Particle Size ◽  
Aluminum Chloride ◽  
Raw Materials ◽  
Alcohol Solution ◽  
Raw Material ◽  
Complexation Reaction ◽  
Optimal Parameters ◽  
Quantitative Content ◽  
Development And Validation

Introduction. Common sorrel (Rumex acetosa L.) is a biennial herb from the Buckwheat family (Polygonaceae L.). In our previously studies of phenolic compounds composition of alcohol extraction and ethereal, ethyl acetate, butanol fractions of common sorrel herba and flavonoids of the flavonol group was found. From the literature sources it is known that flavonoids are one of the groups of biologically active compounds with anti-inflammatory action. In this regard, the actual task is to assess the quantitative content of flavonoids in common sorrel herba.Aim. Is the development and validation of a method for the quantitative determination of flavonoids in common sorrel herba.Materials and methods. It has been established that the flavonoid complex of common sorrel herba includes rutin, therefore, the reference sample (RS) rutin was used as a standard for calculating the amount of flavonoids. Alcohol extract of common sorrel herba and a solution of rutin RS were prepared. The complexation reaction with aluminum chloride was carried out. The resulting solutions were investigated by the method of differential spectrophotometry. The spectral characteristics of the test and standard samples were compared. Then we studied the effect of extraction conditions on the yield of flavonoids from the raw material: the extractant, the particle size of the raw material, the ratio of «raw material – extractant», temperature, frequency and duration of extraction. Purified water and ethyl alcohol of various concentrations (20%, 40%, 70%, 90%) were used as the extractant. Next, carried out the selection of the optimal conditions for the complexation reaction (the complexation reaction time, the ratio of «aliquot – aluminum chloride alcohol solution»). The method was validated according to GPM.1.1.0012.15 of the State Pharmacopoeia (SF) XIII edition and generally accepted methods for the following indicators: specificity, analytical field, linearity, accuracy, precision.Results and discussion. The optimal parameters for extracting flavonoids from raw materials were determined (threefold extraction with ethyl alcohol 70% in a water bath, the ratio of «raw material – extractant» – 1:30 for 30 minutes, the particle size of the raw material – 2.0 mm). The conditions for the complexation reaction were selected (the ratio «aliquot: aluminum chloride alcohol solution» – 1: 2.5, the complexing agent – aluminum chloride solution 5% alcohol, the appearance of a stable solution color after 40 minutes). When carrying out the validation of the developed method, it was established that the validation characteristics under study are within the acceptance criteria. When analyzing the raw materials harvested in the Altai Territory in different years, it was found that the content of flavonoids in common sorrel herba ranges from 0,596 to 0,632%.Conclusion. The optimal parameters of extraction of flavonoids from raw materials were determined, the conditions for the complexation reaction were selected, and the developed method was validated. The quantitative content of flavonoids in terms of rutin in sorrel sour grass harvested in the Altai Territory in different years has been established.

scholarly journals Antimicrobial Activity of Ultrasonic Extracts of Two Chemotypes of Thymus serpyllum L. of Central Kazakhstan and their Polyphenolic Profiles

2021 ◽  
Vol 9 (A) ◽  
pp. 61-67
Author(s):  
Svetlana Ivasenko ◽  
Perizat Orazbayeva ◽  
Krystyna Skalicka–Wozniak ◽  
Agnieszka Ludwiczuk ◽  
Alexandr Marchenko ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wide Spectrum ◽  
Biological Properties ◽  
Climatic Conditions ◽  
Biologically Active ◽  
Gram Positive Bacteria ◽  
Quantitative Content ◽  
Two Samples ◽  
Thymus Serpyllum ◽  
Bacteria And Fungi

BACKGROUND: The medicinal plant of Thymus serpyllum L. in nature, depending on the geographical region, climatic conditions, and growing environment, is represented with some chemotypes. Composition and quantitative content of the basic groups of the biologically active substances can be differed, and thus their biological properties are also various. AIM: The aim of the study was to determine possibility of the using the ultrasonic extracts of two chemotypes of T. serpyllum L. of Central Kazakhstan as an antimicrobial agent against test strains of microorganisms. MATERIALS AND METHODS: Two samples of T. serpyllum were extracted with 70% ethanol using ultrasound. The polyphenol content of the ultrasound extracts was determined using the LC-ultraviolet-ESI- tandem mass spectrometry technique. A study of an antimicrobial activity of the ultrasonic extracts was performed with eight strains of Gram-positive bacteria, six strains of Gram-negative bacteria, and four cultures of fungi. RESULTS: The ultrasonic extracts of two chemotypes of T. serpyllum L. are similar in composition of phenolic compounds but differ in a quantitative content of phenolic acids and flavonoids, except for a rosmarinic acid. The ultrasonic extracts have a wide spectrum of antimicrobial activity, exhibit the bactericidal or bacteriostatic activity against all tested bacteria and fungi at a concentration of 0.0625–20 mg/ml, but differ in their strength of action against test strains of microorganisms. CONCLUSION: The ultrasonic extracts of two chemotypes of T. serpyllum L. of Central Kazakhstan can be considered as a potential drug with a wide spectrum of antimicrobial activity. The results of chromatographic analysis will be used for standardization of a drug.

scholarly journals Functional value of a talgan made from sprouted wheat and barley

2021 ◽  
Vol 83 (1) ◽  
pp. 163-168
Author(s):  
A. V. Sumina ◽  
V. I. Polonskiy ◽  
T. M. Shaldaeva
Keyword(s):  
Total Content ◽  
Barley Grain ◽  
Climatic Conditions ◽  
Point Of View ◽  
Raw Material ◽  
National Product ◽  
Bidistilled Water ◽  
Functional Value ◽  
Germination Process ◽  
The Republic

The aim of this study was to analyze the total content of antioxidants (TCA) at different stages of production of the Khakass national product talgan, made from sprouted wheat and barley. Grain crops were grown on the territory of the Bey district of the Republic of Khakassia, which is characterized by favorable climatic conditions from the point of view of growing grain with an increased content of antioxidants. To determine the TCA value in the grain, two solvents were used – hot bidistilled water and 70% ethanol. The TCA measurement was performed on the device "Color Yauza-01-AA". Among all the grain samples studied, higher values were recorded when using hot bidistilled water as an eluent. It was found that the germination process did not reduce the taste qualities of the grain product. The value of the TCA in talgan, made on the basis of sprouted barley grain, was higher in comparison with wheat by 34%. The effect of increasing the level of TCA in the product in comparison with the initial raw material after the stage of grain germination in wheat and barley, respectively, by 1.5 and 2 times, and after the stages of roasting and grinding by 2 and 2.5 times. The value of FSA in bran exceeded that in the original grain by 44 and 65% for wheat and barley, respectively. Thus, as a result of the research, it was found that regardless of the studied crop (wheat, barley), the process of germination of grain is one of the effective ways to increase the value of TCA in the talgan product made on its basis.

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