scholarly journals COMPREHENSIVE STUDY OF THE PROFILE OF FREE ORGANIC ACIDS IN FRUITS OF SEA BUCKTHORN (HIP-POPHAE RHAMNOIDES L.) OF VARIOUS VARIETIES

2021 ◽  
pp. 231-240
Author(s):  
Ol'ga Valer'yevna Trineeva ◽  
Margarita Aleksandrovna Rudaya
Keyword(s):  
Organic Acids ◽  
Complete Information ◽  
Sea Buckthorn ◽  
Biologically Active ◽  
Specific Marker ◽  
Standard Samples ◽  
Tartaric Acids ◽  
Close Relationship ◽  
Layer Chromatography

An important role in the metabolism of ripening plant fruits is assigned to such a group of biologically active substances (BAS) as organic acids. This group of compounds accumulates in the fruits of plant objects in significant quantities, and the nature of the accumulation is in close relationship with metabolic processes, and is also determined by the influence of environmental factors, species and varietal characteristics of the plant. One of the promising plant sources of this group of BAS is the fruits of sea buckthorn (Hippophae rhamnoides L.). On the example of the fruits of ten different varieties of this plant, a comparative study of the profile of free organic acids was carried out using a complex of different methods of analysis (capillary electrophoresis (CE), thin layer chromatography (TLC), titrimetry). The cultivars with the maximum and minimum accumulation of BAS were identified. It was found that the composition of organic acids for the representatives of the variety samples is not the same, the maximum separation of chromatographic zones is observed in the variety «Ryabinovaya», the minimum - in the varieties «Stolichnaya», «Galerit», «Botanicheskaya», «Botanicheskaya amateur». The studied fruits were found to contain oxalic, malic, succinic and tartaric acids, identified using reliable standard samples. In the course of the research, specific marker zones were established, which can characterize the belonging of the fruits to a particular variety. The most informative is the method of СE, as it allows for one analytical procedure to carry out the qualitative and quantitative determination of free organic acids in acid and salt forms. However, complete information on the composition and quantitative content of acids can be obtained only by combining TLC and CE methods.

scholarly journals DETERMINATION OF SIMPLE SUGARS IN MEDICINAL PLANT MATERIALS BY THE METHOD OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY (FOR EXAMPLE, THE FRUITS OF SEA BUCKTHORN L. AND NETTLE LEAVES L.)

2020 ◽  
pp. 215-222
Author(s):  
Ol'ga Valer'yevna Trineeva ◽  
Aleksey Ivanovich Slivkin
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Raw Materials ◽  
Sea Buckthorn ◽  
Standard Samples ◽  
Plant Materials ◽  
Layer Chromatography

An analysis of the literature over the past 20 years has shown that when controlling the quality of drugs containing monosaccharides, as well as studying the composition of simple sugars in polysaccharide complexes of medicinal plants and not only, preference is given to physicochemical methods, as the most express, sensitive and informative. No means have been found in the scientific literature to identify and quantify simultaneously various monosaccharides by high performance thin layer chromatography (HPTLC). An economical and rapid method has been developed for the identification and quantitative determination of simple reducing sugars (by the example of glucose, rhamnose and xylose) by the HPTLC method. The optimal conditions for their chromatography in a thin layer of sorbent with a quantitative interpretation of HPTLC data on a personal computer were experimentally selected and theoretically substantiated. In a detailed study of the influence of the polarity of the system on the value of Rf, the intervals of values of the polarity of the eluent were chosen, in which these dependences become linear. Using the proposed dependencies, you can select different systems for the separation of monosaccharides in a thin layer of sorbent, so that the value of Rf fit into the optimal values. The proposed method was tested on medicinal plant raw materials of nettle dioica and sea buckthorn fruits of various conservation methods. Zones of simple sugars of characteristic color were found on the chromatograms of extracts from the studied raw materials, among which glucose, xylose and rhamnose were identified by the characteristic value of Rf values in comparison with reliable standard samples. The technique can be used in quality control of substances, single-component and complex preparations, plant objects, dietary supplements, premixes and products of the food industry.

scholarly journals The study of the qualitative composition and the quantitative content of phenolic compounds in dietary supplements with lingonberry

2021 ◽  
Vol 19 (4(76)) ◽  
pp. 40-46
Author(s):  
Oleksandr Yu. Maslov ◽  
Mykola A. Komisarenko ◽  
Serhii V. Kolisnyk ◽  
Olga V. Antonenko ◽  
Olena V. Kolisnyk ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Dietary Supplements ◽  
Total Content ◽  
Hydroxycinnamic Acids ◽  
Biologically Active ◽  
Qualitative Composition ◽  
Relevant Today ◽  
Quantitative Content ◽  
Layer Chromatography

Aim. Today, there are a lot of dietary supplements with lingonberry at the pharmaceutical market of Ukraine; therefore, the analysis and quality control of these products are relevant. In this connection, the aim of the research was to study the qualitative composition and determine the quantitative content of phenolic compounds in dietary supplements with lingonberry.Materials and methods. Such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM) were chosen for the study. The qualitative analysis was performed by thin layer chromatography (TLC), spectrophotometry was used for the quantitative determination.Results and discussion. Hydroquinone derivatives, flavonoids and hydroxycinnamic acids were found in the dietary supplements analyzed. The total content of phenolic compounds was 8.70, 0.26, 0.30 %, flavonoids – 6.37, 0.15, 0.12 %, hydroxycinnamic acids – 0.94, 0.06, 0.13 %, and hydroquinone derivatives – 1.01, 0.04, 0.03 % in such dietary supplements as “Extract of lingonberry” (MEDAGROPROM), “Lingonberry” (Danikafarm), “Lingonberry nano” (LSS SYSTEM), respectively. Conclusions. The qualitative and quantitative analysis of the dietary supplements with lingonberry analyzed has been performed. “Extract of lingonberry” (MEDAGROPROM) dietary supplement meets the requirements of the State of Pharmacopoeia of Ukraine 2.0, whereas “Lingonberry” (Danikafarm) and “Lingonberry nano” (LSS SYSTEM) do not. Based on the results of the study it can be concluded that the problem of compliance of dietary supplements is relevant today and requires the introduction of regulatory documentation for the detection and determination of biologically active substances in dietary supplements.

scholarly journals Identification and determination of ascorbic acid, free organic acids and tannic substances in the grass of Inula L. genus species

2019 ◽  
Vol 7 (1) ◽  
pp. 25-33
Author(s):  
Olena Yerenko ◽  
Galina Smoylovska ◽  
Taya Khortetska
Keyword(s):  
Ascorbic Acid ◽  
Organic Acids ◽  
Quantitative Determination ◽  
Biologically Active ◽  
Raw Material ◽  
Grass Species ◽  
Inula Britannica ◽  
Inula Helenium ◽  
Inula Britannica L

The level of studying of Inula L. genus species, growing on the territory of Ukraine, is insufficient today. The study of chemical composition, accumulation of biologically active substances, pharmacological action of investigated grass species’ extracts of Inula L. genus of Ukrainian flora are of current interest. The aim of the work is making qualitative and comparative analysis of ascorbic, organic acids and tannic substances content in the raw material of investigated species of Inula L. genus (Inula britannica L. and Inula helenium L.). Quantitative determination was carried out by volume titration method. The presence of ascorbic acid, organic acids and tannic substances in the raw material of Inula L. genus investigated species was established with the help of paper chromatography and thin-layer chromatography methods. The quantitative determination of total organic acids was carried out according to XI edition of State Pharmacopoeia methods in terms of malic acid. Water extracts were studied for the tannins presence in raw material of Inula L. genus investigated species. Compounds identification was carried out through chemical reactions in accordance with Ukrainian State Pharmacopeia. The results obtained showed the perspective of further study of the biological activity of Inula britannica L. and Inula helenium L.

scholarly journals Comparative Characteristics of Sugar Determination by Different Methods in Leaves of Nettle

2020 ◽  
Vol 9 (2) ◽  
pp. 91-97
Author(s):  
O. V. Trineeva
Keyword(s):  
Raw Materials ◽  
Physiological Activity ◽  
Gravimetric Method ◽  
Total Content ◽  
Complete Information ◽  
Biologically Active ◽  
Polysaccharide Complex ◽  
Plant Raw Materials ◽  
Spectrophotometric Methods

Introduction. According to the literature, nettle leaves contain polysaccharides and mucus as an integral part of the hydrophilic fraction. This group of biologically active substances (BAS) is involved in the manifestation of the physiological activity of the infusion. The traditional gravimetric method for the determination of polysaccharides in medicinal plant raw materials (PRM), pharmaceutical substances of plant origin and medicinal herbal preparations cannot provide a true picture of their content due to the presence of impurities. Therefore, it is advisable to determine the reducing sugars that are most fully extracted into the aqueous phase during the preparation of the infusion using modern physicochemical methods of analysis.Aim. The aim of the work was to determine the amount of polysaccharides and simple sugars in nettle leaves by various physico-chemical methods and their comparative characteristics.Materials and methods. A comparative determination of the sum of polysaccharides and simple sugars in the leaves of nettle by pharmacopoeial methods (picrine, anthrone and orcine) was carried out. Free and bound simple sugars were identified and quantified by thin layer chromatography (TLC). The composition and content of free simple sugars was studied by capillary electrophoresis (CE) in raw materials.Results and discussion. The content of free and bound sugars in raw materials has been established. The largest content of both the sum of polysaccharides and free simple sugars in the polysaccharide complex of nettle leaves, as well as the sum of free reducing simple sugars, according to experimental data, was obtained using the picrine method. The content of pentoses in the studied PRM was four times less, which is consistent with the literature data on the preferential construction of polysaccharides of plant objects from sugars of the hexose class. The resulting total content of the fraction of free sugars in the leaves, determined by the CE method, is an order of magnitude less compared with spectrophotometric methods. The obtained results of quantitative determination of the amount of free and bound simple sugars in the studied PRM are consistent with the determination data by the picrine pharmacopoeial method.Conclusion. In general, complete information, depending on the goals of the analysis, on the composition and quantitative content of simple and related sugars in the polysaccharide complex of plant objects can only be obtained by combining spectral methods with CE or TLC.

scholarly journals DETERMINATION OF BIOLOGICAL ACTIVITY OF SEA BUCKTHORN OILS AND EXTRACTS FROM THE CON-TENT OF LIPID ANTIOXIDANTS VIA MICROCALORIMETRY

2020 ◽  
pp. 223-230
Author(s):  
Nataliya Vital'yevna Sizova
Keyword(s):  
Antioxidant Activity ◽  
Sea Buckthorn ◽  
Biologically Active ◽  
Dry Extract ◽  
Antioxidant Content ◽  
Therapeutic Value ◽  
Lipid Fractions ◽  
Carbon Dioxide Extraction

An alternative method for assessing the quality of sea buckthorn oils and extracts from the concentration of biologically active lipid substances exhibiting antioxidant activity is considered. The method of microcalorimetry is used to assess the content of antioxidants. The heat of a model cumene oxidation reaction in the presence of sea buckthorn oils and extracts obtained by various technologies is registered. As compared to other methods for obtaining sea buckthorn lipid fractions, the extraction of sea buckthorn fruits with sunflower oil is considered to be an unproductive method, since an increase in antioxidant activity compared with the initial oil is found to be moderate: from 1.56∙10-3 to 1.69∙10-3 mol/kg. Sea buckthorn oil obtained by pressing berries (antioxidant content 6.12∙10-3 mol/kg) and carbon dioxide extraction of sea buckthorn meal (antioxidant content 5.92∙10-3 mol/kg) are of the highest biological value among the objects under study. The maximum amount of antioxidants (5.5∙10-2 mol/kg) is determined in a dry extract of sea-buckthorn fruits enriched with triterpenic acids of the ursan and olean series. The method for assessing the quality of extracts used in the work allows choosing the most efficient technique for processing sea buckthorn fruits. The concentration of antioxidants consisting of a complex of tocopherols, carotenoids, triterpenoids, lipophilic acids, and other compounds is a good criterion of the quality and therapeutic value of oils.

scholarly journals Validation of the alkalimetry method for the quantitative determination of free organic acids in raspberry leaves

2021 ◽  
Vol 19 (1(73)) ◽  
pp. 53-58
Author(s):  
O. Yu. Maslov ◽  
S. V. Kolisnyk ◽  
T. A. Kostina ◽  
Z. V. Shovkova ◽  
E. Yu. Ahmedov ◽  
...  
Keyword(s):  
Organic Acids ◽  
Quantitative Determination ◽  
Biologically Active ◽  
Intermediate Precision ◽  
Potentiometric Detection ◽  
Class A ◽  
Ich Guidelines ◽  
Relative Standard ◽  
Intermediate Metabolites

 Organic acids are a large group of biologically active compounds that perform important functions in the plant organism. Moreover, all plants, regardless of the species and family, contain organic acids to a small or large extent as organic acids belong to intermediate metabolites arising from the oxidation of proteins and amino acids, fats and carbohydrates.Aim. To validate the method of alkalimetry proposed with potentiometric detection of the end-point for the quantitative determination of free organic acids in raspberry leaves.Results and discussion. The method proposed was validated according to the International Conference on Harmonization (ICH) guidelines. The linearity was in the concentration range of 40 – 200 % (r2 = 0.9991). The percentage of recovery was found to be in the range of 98.77 – 102.48 %. The repeatability and intermediate precision were 1.58 % and 1.74 %, respectively. The method is accurate and reliable, with the relative standard deviation of less than 2 %.Experimental part. Leaves of raspberry were collected in the Kharkiv region during the period of full ripening. A Hanna 2550 pH-meter with a HI 1131P potentiometric electrode was used for alkalimetric titration of free organic acids. The titration was carried out using a microburette with Class A accuracy.Conclusions. The alkalimetry method for the quantitative determination of free organic acids in raspberry leaves has been proposed and validated according to the following parameters: specifcity, linearity, accuracy, repeatability, intermediate precision, robustness. It has been confrmed that the method is simple, reliable, accurate and cost-effective.Key words: raspberry; leaves; free organic acids; alkalimetry; validation

scholarly journals Identification and quantitative determination of the flavonoids of the complex dense extract of st. john's wort herb and pot marigold flowers

2018 ◽  
pp. 71-79
Author(s):  
T. A. Shostak ◽  
T. G. Kalyniuk ◽  
L.V. Vronska
Keyword(s):  
Active Pharmaceutical Ingredient ◽  
Biologically Active ◽  
Chromatography Method ◽  
St John’S Wort ◽  
St John's Wort ◽  
Chromatographic Profile ◽  
Pot Marigold ◽  
Layer Chromatography

Common Saint-John's wort (Hypericum perforatum) and pot marigold (Calendula officinalis) are rich in such biologically active substances (BAS) as carotene, ascorbic acid, essential oils, vitamins, tannin and resinous substances, as well as flavonoids that bear evident wound healing properties and antiulcerous properties. The object of this study was BAR composition of the complex dense herb extract of St. John's wort and flowers of marigolds (1:10). In order to introduce a new herbal substance into medical practice, it is necessary to develop methods for its identification and quantification. The TLC [thin layer chromatography] method was used to identify the BAR in the extract under study, and the method of absorption spectrophotometry was proposed for quantification of the content of flavonoids. As a result of the conducted research, there were selected characteristic substances - identification markers of the extract, the choice of which was in accordance with the requirements of the SPF on the quality of the herb of St. John's wort and the flowers of pot marigold, and there was indicated the position and coloring of the zones in the chromatographic profile of the tested extract solution. Such approach will enable objective identification of the extract as a substance and as an active pharmaceutical ingredient in the formulation. The criterion for quantitative standardization of the complex dense extract is the content of the amount of flavonoids not less than 1.5% in terms of hyperoside and dry substance.

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