scholarly journals Evaluation of the antifungal activity of plant extracts and oral antiseptics against Candida albicans

2014 ◽  
Vol 17 (4) ◽  
pp. 98
Author(s):  
Roberta Carvalho Sthel Balliana ◽  
Cláudia Masrouah Jamal ◽  
Simone Bravim Maifrede ◽  
Mariceli Lamas de Araújo
Keyword(s):  
Candida Albicans ◽  
Plant Extracts ◽  
Inhibitory Concentration ◽  
Oral Candidiasis ◽  
Cetylpyridinium Chloride ◽  
Biofilm Inhibition ◽  
Antifungal Effect ◽  
Arctium Lappa ◽  
Potential Use ◽  
Isolated Species

<p><strong>Objective: </strong>Oral candidiasis is the most common fungal infection of the oral cavity, and <em>Candida albicans</em> is the most frequently isolated species. <strong>Material and Methods:</strong> In this study, the potential antifungal effect of extracts from <em>Gossypium hirsutum </em>L., <em>Arctium lappa</em>,<em> Equisetum </em>sp<em>.</em>, <em>Cecropia pachystachya </em>Trécul and <em>Pogostemon heyneanus</em> plants were evaluated on non-adhered cells of <em>C. albicans</em>, and the effect of oral antiseptics A (cetylpyridinium chloride 0.500 mg), B (chlorhexidine gluconate 0.12%), C (hydrogen peroxide 1.5%) and D (thymol, eucalyptol, menthol and methyl salicylate) were evaluated on non-adhered cells and biofilms of C. albicans, using turbidimetry and minimal inhibitory concentration (MIC) of the extracts and the maximal inhibitory dilution (MID) of the antiseptics. <strong>Results:</strong> The most promising results on non-adhered cells were obtained with <em>Cecropia pachystachya</em>Trécul extracts, with MIC values between 7.81 and 3.91 µg/mL. Antiseptics A and B showed the lowest MID values, between 0.20 and 0.10% (Kruskal-Wallis, p&lt;0.0001). Regarding biofilm inhibition, the MID values found were similar for the tested antiseptics, varying from 50% to 0.20% (Kruskal-Wallis, p=0.6915). <strong>Conclusion:</strong> These results show that some plant extracts has potential use in the prevention and treatment of oral candidiasis. </p>

scholarly journals Antifungal effect of polymeric films containing the essential oil of Schinus terebinthifolius on Candida albicans biofilms

2018 ◽  
Vol 14 (27) ◽  
Author(s):  
Hamdia A. Kassim ◽  
Karina Scheuermann ◽  
Alinne D. M. Gomes ◽  
Rubén D. Sinisterra ◽  
Maria E. Cortés
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Oral Candidiasis ◽  
Hydroxypropyl Methylcellulose ◽  
Polymeric Films ◽  
New Drugs ◽  
Schinus Terebinthifolius ◽  
Antifungal Effect

Introduction: Candida albicans is a commensal microorganism in humans, which can cause oral candidiasis in people with immune deficiencies or ill-fitting dentures. C. albicans is becoming more resistant to the drugs used in its treatment, causing the need for the development of new drugs, which would not damage the host´s cells. There are many studies on the antimicrobial activity of the leaves, stem and roots of Schinus terebinthifolius, with few studies about its fruits. The aim of this study was to investigate the antifungal effect of polymeric films of hydroxypropyl methylcellulose (HPMC) containing the essential oil of the fruits of S. terebinthifolius against biofilms of C. albicans. Materials and methods: The oil was prepared from the fruits of S. terebinthifolius and incorporated into polymeric films of HPMC using concentrations of 0.146, 0.073 e 0.037 g.mL-1. The minimum inhibitory concentration (MIC) was determined by micro dilution, wherein the minimum inhibitory concentration was 0.78 %. The antifungal effect of the films was tested using agar in petri dishes and acrylic resin blocks, both contaminated with C. albicans. Results: The polymeric films containing the essential oil had average inhibition zones of 24 mm, 21 mm and 20 mm for the concentrations 0.037, 0.073 and 0.146 g.mL-1, respectively. Conclusion: The treated resin blocks showed no fungal growth afterwards, with the exception of the control blocks. The use of antimicrobial films containing natural products like S. terebinthifolius in the treatment of diseases such as oral candidiasis associated to prosthetic seems promising decreasing the possibility of side effects.

scholarly journals Synergistic Effect of Plant Extracts on Endodontic Pathogens Isolated from Teeth with Root Canal Treatment Failure: An In Vitro Study

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 552
Author(s):  
Suraj Arora ◽  
Shahabe Saquib ◽  
Youssef Algarni ◽  
Mohammed Kader ◽  
Irfan Ahmad ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Synergistic Effect ◽  
Enterococcus Faecalis ◽  
Plant Extracts ◽  
Azadirachta Indica ◽  
Inhibitory Concentration ◽  
Pathogenic Microorganisms ◽  
Salvadora Persica ◽  
Actinomyces Naeslundii

Background and objectives: This study aimed to evaluate the synergistic antimicrobial activity of extracts obtained from Salvadora persica (Miswak), Commiphora molmol (myrrh) and Azadirachta indica (neem) in combination with commercially available antimicrobial agents: penicillin, tetracycline, ofloxacin and fluconazole on endodontic pathogens such as Enterococcus faecalis, Streptococcus mitis, Actinomyces naeslundii and Candida albicans. Materials and Methods: Microbiological samples from the root canals of the teeth undergoing retreatment were taken using sterile paper points kept at full length in the canal for 30 seconds. The disc diffusion method was used to check the susceptibility of microbes to the plant extracts and antimicrobials by measuring the diameter of the inhibition zones. Against the microbes, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)/minimum fungicidal concentration (MFC) of the plant extracts were assessed. The fractional inhibitory concentration index (FICI) was used to estimate the synergistic effect of plant extracts combined with antimicrobials against the resistant endodontic microbes. Results: The findings clearly indicate the effectiveness of all the three plant extracts, Commiphora molmol, Azadirachta indica, Salvadora persica, against all the experimental pathogenic microorganisms except for the ineffectiveness of Azadirachta indica, Salvadora persica against Candida albicans. Maximum antimicrobial activity was displayed by Azadirachta indica against Enterococcus faecalis (MIC = 0.09 ± 1.2 mg/mL, MBC = 0.78 ± 1.25 mg/mL) and the minimum antimicrobial activity was displayed by Commiphora molmol against Actinomyces naeslundii (MIC = 12.5 ± 3.25 mg/mL, MBC = 100 ± 3.75 mg/mL). The best synergy was displayed by Commiphora molmol with fluconazole against Candida albicans (FICI = 0.45). Conclusions: The current study delineates the variable antimicrobial activity of plant extracts against the experimental endodontic pathogenic microorganisms. Plant extracts in conjunction with various antimicrobials can be valuable aids in combating relatively resistant endodontic microorganisms that have been the cause of worry in recent years, leading to failure even in treatment procedures following all required protocols.

scholarly journals ANTI-CANDIDA ALBICANS ACTIVITY OF THE ASSOCIATION OF CITRONELAL WITH ANFOTERICIN B OR WITH CETOCONAZOLE

2019 ◽  
Vol 16 (31) ◽  
pp. 250-257
Author(s):  
Patrícia Duarte Costa SILVA ◽  
Brenda Lavínia Calixto dos SANTOS ◽  
Gustavo Lima SOARES ◽  
Wylly Araújo de OLIVEIRA
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Amphotericin B ◽  
Inhibitory Concentration ◽  
Fungal Infections ◽  
Pathogenic Fungi ◽  
Mortality Rates ◽  
New Drugs ◽  
High Morbidity ◽  
Isolated Species

Fungal infections caused by species of the genus Candida are responsible for high morbidity and mortality rates, mainly affecting immunocompromised individuals. Among fungi, Candida albicans is the most frequently isolated species of clinical specimens. A problem associated with increased resistance of pathogenic fungi to the agents used in the therapeutic regimen and the limited number of drugs to cure these infections. As a result, the search for new drugs with antifungal activity has become increasingly important. The aim of this study is to study the antifungal activity of citronellal alone and in combination with amphotericin B or ketoconazole. The Minimal Inhibitory Concentration of citronellal, amphotericin B and ketoconazole against strains of Candida albicans were evaluated by the microdilution technique, and the Minimum Fungicide Concentration of citronellal against the same strains was also performed. Through the checkerboard methodology the effect of the combination of citronelal with amphotericin B or with ketoconazole was determined. This study showed that the association of citronellal with ketoconazole was shown to be an additive against one of the strains of C. albicans and indifferent to another strain. While the combined activity of citronellal and amphotericin B demonstrated an indifferent effect on the strains tested.

scholarly journals Antifungal Activity of Geraniol on Candida albicans Isolates of Pediatric Clinical Importance

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Lima A L A ◽  
Pérez A L A L ◽  
Sousa J P ◽  
Pinheiro L S ◽  
Oliveira-Filho A A ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Cell Walls ◽  
Cell Membranes ◽  
Inhibitory Concentration ◽  
Clinical Importance ◽  
Broth Microdilution ◽  
Antifungal Effect ◽  
Minimum Fungicidal Concentration

Geraniol is a plant-derived monoterpene alcohol that has antifungal effect. The aim of this study was to evaluate the geraniol for antifungal activity against Candida albicans isolates of pediatric clinical importance. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were determined by the broth microdilution techniques. We also investigated possible geraniol action on cell walls (0.8M sorbitol) and cell membranes (Geraniol to ergosterol binding). For 90% of isolates, the MIC and MFC of the phytochemical was 64

scholarly journals In vitro study of antifungal effect of carica papaya peel var. California extract against Candida albicans

2021 ◽  
Vol 8 (1) ◽  
pp. 61-68
Author(s):  
Trisnawaty K ◽  
Anin Esta Rauna ◽  
Siti Rusdiana Puspa Dewi ◽  
Pudji Handayani
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Ethanol Extract ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Control Group ◽  
Antifungal Effect ◽  
Peel Extract

Candida albicans is a normal microflora in the oral cavity which can be an opportunistic pathogen that could cause oral candidiasis when there are underlying predisposing conditions. Papaya as one of the most widely grown herbal plants in Indonesia has been known to cure diseases. Unused papaya peel only became a waste; however, the papaya peel is known to contain active compounds that have antifungal effect, especially raw ones. This study aimed to determine the antifungal effect of raw papaya peel extract of Californian variety and determine the minimum inhibitory concentration (MIC) of this extract on clinical isolates of C. albicans. This study was an experimental laboratory study with post-test only control group design. Ethanol-based extract of papaya peel was obtained from maceration process using 96% ethanol. Antifungal effect testing was carried out in vitro using disc diffusion method (Kirby-Bauer) on clinical isolates of C. albicans. The concentration of Ethanol extract of papaya peel used consisted of 2.5%, 5%, 10%, and 20% with positive control (nystatin) and negative control (distilled water). The results showed that 2.5%, 5%, 10%, and 20% papaya peel extracts were able to inhibit the growth of C. albicans with 2.5% concentration of raw papaya peel extract as minimum inhibitory concentration. Ethanol extract of raw papaya peel of Californian variety with 2.5%, 5%, 10%, and 20% concentrations has small antifungal effect against clinical isolates of C. albicans.

scholarly journals Kadar hambat minimum dan kadar bunuh minimum ekstrak etanol lengkuas merah (Alpinia galanga L.) terhadap pertumbuhan Candida albicansThe minimum inhibitory concentration and a minimum lethal dose of red galangal (Alpinia galanga L.) ethanolic extract on the growth of Candida albicans

2020 ◽  
Vol 32 (1) ◽  
pp. 1
Author(s):  
Harunai Kamoda ◽  
Shelly Lelyana ◽  
Vinna Kurniawati Sugiaman
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Oral Candidiasis ◽  
Lethal Dose ◽  
Ethanolic Extract ◽  
Alpinia Galanga ◽  
Post Hoc ◽  
Minimum Lethal Dose ◽  
Colony Counter

Pendahuluan: Kandidiasis rongga mulut merupakan infeksi yang disebabkan oleh jamur patogen Candida albicans, dengan prevalensi cukup tinggi yaitu 20% - 75%. Obat antijamur yang umum digunakan untuk pengobatan kandidiasis rongga mulut adalah nistatin, akan tetapi nistatin memiliki efek samping dan harganya relatif mahal. Sebagai alternatif lain digunakan tanaman obat seperti lengkuas merah (Alpinia galanga L) yang diduga memiliki aktivitas antijamur. Tujuan penelitian ini adalah untuk mengetahui kadar hambat minimum (KHM) dan kadar bunuh minimum (KBM) ekstrak etanol lengkuas merah terhadap pertumbuhan Candida albicans. Metode: Jenis penelitian eksperimental murni dengan membandingkan kelompok uji yang mengandung ekstrak etanol lengkuas merah (alpinia galanga L) dengan konsentrasi 200mg/ml; 100mg/ml; 50mg/ml; 25mg/ml; 12,5mg/ml; 6,25mg/ml; kontrol positif berupa nistatin, dan kontrol negatif berupa DMSO 10% terhadap pertumbuhan Candida albicans dengan mengamati kekeruhan larutan pada 96 well plate menggunakan spectrophotometer  dan KBM ditentukan menggunakan colony counter dengan pengulangan sebanyak 4 kali. Analisis data menggunakan uji parametrik ANOVA dan uji Post Hoc Tuckey. Hasil: Hasil uji ANOVA nilai p 0,00 yang artinya bahwa kedua data memiliki nilai yang signifikan antar perlakuan, dan uji Post Hoc Tuckey memperlihatkan terdapat perbedaan yang signifikan pada setiap kelompok uji terhadap jumlah Candida. Simpulan: Kadar Hambat Minimum (KHM) ekstrak etanol lengkuas merah (Alpinia galanga L) terhadap pertumbuhan Candida albicancs adalah 200mg/ml namun hanya menghambat 60%, sedangkan ekstrak etanol lengkuas merah (Alpinia galanga L) tidak dapat membunuh Candida albicans.Kata kunci: Candida albicans, lengkuas merah, Alpinia galanga L. ABSTRACTIntroduction: Candida albicans is a pathogenic fungus that can cause oral candidiasis, reaches 20% - 70% prevalence in human. Antifungal drugs often used for oral candidiasis treatment is nystatin, but nystatin has many harmful side effects, and its price is relatively high. Therefore, herbal remedies such as red galangal (Alpinia galanga L.), which presumed to have antifungal activity, can be used as an alternative treatment. The purpose of this research was to determine the minimum inhibitory concentration and a minimum lethal dose of red galangal ethanolic extract on the growth of Candida albicans. Methods: This research was an experimental laboratory, conducted by comparing the test group containing ethanolic extracts of red galangal (Alpinia galanga L.) with the concentrations of 200mg/ml; 100mg/ml; 50mg/ml; 25mg/ml; 12.5mg/ml; and 6.25mg/ml; positive control group was treated with nystatin; and negative control with 10% DMSO. The solutions on 96 well plates were observed with a spectrophotometer, and the minimum killing concentration (MKC) was determined using a colony counter, and the experiment was replicated four times. The result was then analysed with ANOVA parametric test and the Post-Hoc Tuckey test afterwards. Results: The result of the ANOVA test showed that the p-value was < 0.05, which means that all treatments have a significant value, while the Post-Hoc Tuckey test indicated that there were significant differences in each group regarding the number of Candida albicans. Conclusion: Minimum Inhibitory Level (MIC) of red galangal (Alpinia galanga L) ethanolic extract against Candida albicans growth is 200mg/ml but only inhibits 60% of the growth. In contrast, the extract is unable to mortally affects the Candida albicans.Keywords: Candida albicans, red galangal, Alpinia galanga L.

scholarly journals ANTIFUNGAL EFFECT OF MAULI BANANA STEM EXTRACT, BASIL LEAF EXTRACT, AND THEIR COMBINATION ON Candida albicans

2020 ◽  
Vol 5 (2) ◽  
pp. 179
Author(s):  
Hadi Waskito ◽  
Maharani Laillyza Apriasari ◽  
Juliyatin Putri Utami
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Oral Candidiasis ◽  
Inhibition Zone ◽  
Chlorhexidine Gluconate ◽  
Diffusion Method ◽  
Antifungal Effect ◽  
Group I ◽  
Stem Extract ◽  
Banana Stem

ABSTRACTBackgrounds: Oral candidiasis is an oral infection caused by abnormal growth of Candida albicans. The use of 0.2% chlorhexidine gluconate as a prophylactic mouthwash is conducted for oral candidiasis therapy. Prolonged use of 0.2% chlorhexidine gluconate is recorded to instigate side effects. Mauli banana stem extract and basil leaf extract demonstrate antifungal properties ascribed to their contents. Objective: To prove that the antifungal effect of 25% concentration of mauli banana stem extract, 12.5% concentration of basil leaf extract, and 25%, 50%, and 75% concentration of mauli banana stem and basil leaf combination extract on Candida albicans are comparable to 0.2% chlorhexidine gluconate. Methods: This study was resolved by applying the true experimental design with post-test only and control group design which consisted of 6 treatments. Group I was given Mauli banana stem extract with 25% concentration, group II was given basil leaf extract with 12.5% concentration, group III, IV, and IV were given the combination of Mauli banana stem and basil leaf extracts with 25%, 50%, and 75% concentrations, respectively. Meanwhile, group VI was given 1% chlorhexidine gluconate as a positive control. Each treatment was served with four times repetition. Antibacterial effectivity was assessed through the inhibition zone measurement of 0.2% chlorhexidine gluconate growth using the diffusion method. Results: This study revealed that the average diameter of inhibition zone formed in group I was 6-6.3 mm, group II was 6-6.2 mm, group III was 6-6.2 mm, group IV was 7-7.2 mm, group V was 10.2-11.4 mm, and group VI was 23.7-24.8 mm. Conclusion: The antifungal effect of mauli banana stem extract at 25% concentration, basil leaf extract at 12.5% concentration, and mauli banana stem and basil leaf combination extract at 25%, 50%, and 75% concentration on Candida albicans has been proven but not equivalent to 0.2% chlorhexidine gluconate.Keywords: Basil leaf extract, Candida albicans,inhibition zone, 0.2% chlorhexidine gluconate, diffusion method, Mauli banana stem extract,.

scholarly journals In Vitro Antifungal Susceptibility Testing of Tea Tree Oil (TTO) 5% Compared with Nystatin against Candida sp. as Important Agent of Oral Candidiasis in HIV/AIDS Patients

2020 ◽  
Vol 32 (3) ◽  
pp. 189
Author(s):  
Eva Lydiawati ◽  
Muhammad Yulianto Listiawan ◽  
Dwi Murtiastutik ◽  
Rahmadewi Rahmadewi ◽  
Cita Rosita Sigit Prakoeswa ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Candidiasis ◽  
Antifungal Susceptibility ◽  
Inhibition Zone ◽  
Tea Tree Oil ◽  
Aids Patients ◽  
Antifungal Effect ◽  
Tea Tree ◽  
Candida Sp ◽  
Hiv Aids

Background: Oral candidiasis is the most common clinical manifestation in a person infected with HIV which has been linked with the severity of the disease. Candida albicans responsible for most oral candidiasis. The increased resistance to the available antifungal drugs can reduce the efficacy of therapy. Therefore, it will be crucial to discover novel antifungal agents. One of them is tea tree oil (TTO) or Melaleuca alternifolia which has a potent antifungal effect to inhibit the growth of Candida sp.  Purpose: To evaluate the antifungal susceptibility of TTO 5% compared with nystatin against Candida sp. taken from HIV/AIDS patients. Methods: The study assessed the antifungal effect of TTO 5% and nystatin against 33 isolates of Candida sp. that was isolated from the oral cavity of 30 HIV/AIDS patients in Dr. Soetomo General Academic Teaching Hospital Surabaya, Indonesia. Antifungal susceptibility was evaluated by the disc diffusion method. The diameter of the inhibition zone was determined as the result of this study. Result: The most strains found in this study was Candida albicans (84.8%). The mean inhibition zone of nystatin against all strains was 23.24 mm whether it was 17.55 mm for TTO 5%. The inhibition zone of both TTO 5% and nystatin in Candida non-albicans were tended to be higher than in Candida albicans. It revealed that the inhibition zone of TTO 5% significantly lower than nystatin (P = 0.00; P < 0.05).Conclusion: Therefore, the antifungal susceptibility of nystatin was significantly higher than TTO 5% against Candida albicans and non-albicans.

scholarly journals Impact of Brief Exposure to Drugs with Antifungal Properties on the Susceptibility of Oral Candida dubliniensis Isolates to Lysozyme and Lactoferrin

2018 ◽  
Vol 27 (6) ◽  
pp. 523-530 ◽  
Author(s):  
Arjuna Nishantha B. Ellepola ◽  
Ranil Samantha Dassanayake ◽  
Ziauddin Khan
Keyword(s):  
Amphotericin B ◽  
Inhibitory Concentration ◽  
Oral Candidiasis ◽  
Therapeutic Agents ◽  
Candida Dubliniensis ◽  
Antifungal Effect ◽  
Candida Infections ◽  
Oral Candida

Objective: Lysozyme and lactoferrin have anti-candidal activity. Candida dubliniensis is associated with oral candidiasis. Candida infections are managed with nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine. Candida species undergo a brief exposure to therapeutic agents in the mouth. There is no data on the influence of limited exposure to antimycotics on the sensitivity of C. dubliniensis to lactoferrin and lysozyme. Hence, this study observed the changes in the sensitivity of C. dubliniensis to anti-candidal action of lactoferrin and lysozyme after transitory exposure to sub-lethal concentrations of antifungals. Materials and Methods: After determination of the minimum inhibitory concentration (MIC), 20 C. dubliniensis isolates were exposed to twice the concentration of MIC of nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine for 1 h. Drugs were removed by dilution and thereafter the susceptibility of these isolates to lysozyme and lactoferrin was determined by colony-forming unit quantification assay. Results: Exposure of C. dubliniensis to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine resulted in an increase in susceptibility to lysozyme by 9.45, 30.82, 30.04, 50.64, 55.60, and 50.18%, respectively (p < 0.05 to p < 0.001). Exposure of C. dubliniensis to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine resulted in an increase in susceptibility to lactoferrin by 13.54, 16.43, 17.58, 19.60, 21.32, and 18.73, respectively (p < 0.05 to p < 0.001). Conclusion: Brief exposure to nystatin, amphotericin B, caspofungin, ketoconazole, fluconazole, and chlorhexidine enhances the antifungal effect of lysozyme and lactoferrin on C. dubliniensis isolates in vitro.

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