scholarly journals PROPUESTA DE UNA METODOLOGÍA PARA CUANTIFICAR LA ACTIVIDAD ANTIBACTERIANA DE LA MIEL

2021 ◽  
Vol 19 (suplemento) ◽  
Author(s):  
A Ferrer
Keyword(s):  
Stainless Steel ◽  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Agar Plate ◽  
Test Organism ◽  
Diffusion Method ◽  
Medicinal Purpose ◽  
Inhibitory Zone ◽  
Petri Dishes ◽  
The Relationship

In this study a standardized method for objectively comparing the antibacterial activity of honey was developed. The assay was performed using a strain of S. aureus and four honeys that have been shown antibacterial activity against this strain. The antibacterial activity of honeys were checked by using an agar plate diffusion method using S. aureus (ATCC 29213) as the test organism. On the surface of each agar plate six stainless steel cylinders with a diameter of 8,14 mm were radially disposed. Onto each cylinder 0,1 mL of sample honey at 10% v/v was placed and all Petri dishes were incubated at 35°C during 24 h. Each sample were analyzed by triplicate. The surface of the cylinder diameter was set as reference antibacterial activity equivalent to minimal inhibitory concentration (MIC). The antibacterial activity of each honey was quantitated by the relationship between the surface of the inhibitory zone and the surface of the reference activity expressed as multiples of MIC (x MIC). The method allowed establishing differences between the bacterial activities of the different honeys tested and could be used for identifying honeys that could be employed with medicinal purpose for the treatments of wounds.

scholarly journals DILUCIÓN DE MIEL EN AGUA INDUCE HORMESIS EN LA ACTIVIDAD ANTIBACTERIANA SOBRE Staphylococcus aureus.

2021 ◽  
Vol 19 (suplemento) ◽  
Author(s):  
R Castromán
Keyword(s):  
Antibacterial Activity ◽  
Agar Plate ◽  
Test Organism ◽  
Diffusion Method ◽  
Low Doses ◽  
High Efficacy ◽  
Inhibitory Zone ◽  
The Relationship ◽  
Higher Doses

In pharmacology, hormesis is defined as the high efficacy of a drug at low doses and a decrease of efficacy at higher doses. In this study, the hormesis phenomenon on the antibacterial activity of honey was assessed. The activity of four honeys (A, D, C and D) were checked by agar plate diffusion method using S. aureus (ATCC 29213) as the test organism. Each honey was tested as follows; on the surface of an agar plate, five stainless steel cylinders with a diameter of 8,14 mm were radially disposed. Onto each cylinder 0,1 mL of the sample honey diluted in water at 50 – 25 - 12,5 -6,25 and 3,125 % v/v were placed. Plates were incubated at 35°C during 24 h. The surface of the cylinder diameter was set as reference antibacterial activity (Ra). The antibacterial activity of each honey was quantitated by the relationship between the surface of the inhibitory zone (Iz) and the surface of the reference activity (Iz/Ra). An increase in the antibacterial activity was observed at a concentration of water greater than 50% in honeys B and C. This hormetic behavior could be considered as indicator of the quality of the antibacterial activity of these honeys.  

In-Vitro Studies on the Sensitivity of Staphylococcus aureus to Some Ethno-Medicinal Preparations Sold around Kano, Nigeria

2011 ◽  
Vol 4 (1) ◽  
pp. 22-25 ◽  
Author(s):  
M Bashir ◽  
I Yusuf ◽  
AS Kutama
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Test Organism ◽  
Ethanolic Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Herbal Preparations

Five traditional herbal preparations were sampled between May-June, 2009 in Kano. The samples were investigated for invitro antibacterial activities against clinical isolates of Staphylococcus aureus. Likewise, phytochemical screening tests were conducted to determine some of the phytochemicals present in the ethanolic and water extracts of the samples. Various concentrations of the extracts were prepared using serial doubling dilutions (5000=l/ml, 2500=g/ml, 1250=g/ml, 625=g/ml and 312.5=g/ml). All the test extracts showed slight antibacterial activity against the test organism, with ethanolic extract of sample E having the highest zone diameter of inhibition, while sample H had the lowest diameter of inhibition. The standard antibiotic disc (Gentamicin) had demonstrated the highest activity on the test organisms. The results of the Phytochemical screening revealed the presence of steroid in all the samples, tannin in samples A, C, D and E, reducing sugars in sample A, D and E respectively. The result of the minimum inhibitory concentration (MIC) was found to be above 312.5=g/ml for samples C, D and E. Keywords: Staphylococcus aureus, Herbal preparations, antibacterial activity, Phytochemical screening and minimum inhibitory concentration.

scholarly journals AKTIVITAS SEDIAAN GEL KUERSETIN TERHADAP Staphylococcus Epidermidis

2015 ◽  
Vol 1 (1) ◽  
pp. 59-64
Author(s):  
Rina Apriana Herslambang ◽  
Dina Rahmawanty ◽  
Mia Fitriana
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Phenolic Compound ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Sebaceous Glands ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Acne is the abnormality production of sebum in the sebaceous glands which are usually caused by Staphylococcus epidermidis. Staphylococcus epidermidis one of bacterial that caused acne. Quercetin have a phenolic compound that can inhibit the growth of Staphylococcus epidermidis at 0.05% w/w. The purpose of this study was to determine the minimum inhibitory concentration of quercetin gel. HPMC was used as gel base. The concentrations of quercetin that was added to gel were 0.05% w/w, 0.15% w/w, and 0.25% w/w. The antibacterial activity of the gels was determined using agar diffusion method. The results showed that the inhibition zone on the F1, F2, and F3 were 7.83 mm , 6.53 mm , 4.56 mm and the result showed that quercetin gels significantly effected the growth of Staphylococcus epidermidis (p<0,05). The minimum inhibitory concentration of quercetin gel was 0,05% w/w with inhibition zone of 7.83 mm.

scholarly journals Antibacterial Activity Of Tangerine Powder (Citrus reticulata Blanco.) Against Caries-Causing Bacteria (Streptococcus mutans)

2020 ◽  
Vol 10 (2) ◽  
pp. 148-157
Author(s):  
Framesti Frisma Sriarumtias ◽  
Aji Najihudin ◽  
Nopi Rantika ◽  
Rita Nengsih
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Pharmaceutical Ingredients ◽  
Caries Treatment ◽  
Inhibitory Power

Dental caries is caused by Streptococcus mutans, which lives in the oral cavity and causes plaque on the teeth. Dental caries treatment involves restoration or filling of tooth tissue that has been perforated due to dental caries. The purpose of this study was analyze the effectivity of  tangerine juice powder in preventing the emergence of dental caries caused by Streptococcus mutans. The experimental method was used in this study started by making a powder from tangerine jiuce using freeze drying method, followed by testing the antibacterial activity using the disc diffusion method. In addition, the minimum inhibitory concentration (MIC) was also sought by measuring the diameter of the clear zone. The results showed that tangerine powder has antibacterial activity, with a minimum inhibitory concentration of 10%. The highest activity of tangerine powder to inhibit the growth of Streptococcus mutans bacteria was observed at   concentration of 25% with an inhibition zone diameter of 15.8 mm, and is classified as having moderate inhibitory power. According to the result of this study is that tangerine juice powder has a good antibacterial activity against Streptococcus mutans and can be developed into a pharmaceutical ingredients to prevent dental caries.

scholarly journals Screening and Production of Antibacterial from Lactobacillus plantarum NS(9) Isolated from Nile Tilapia Bekasam

2016 ◽  
Vol 19 (2) ◽  
pp. 132
Author(s):  
Desniar Desniar ◽  
Iriani Setyaningsih ◽  
Yoga Indra Purnama
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Ph Value ◽  
Diffusion Method ◽  
Exponential Growth Phase ◽  
Anaerobic Conditions ◽  
Inhibitory Zone ◽  
Antibacterial Compounds ◽  
E Coli ◽  
Growth Of Bacteria

<p>Lactic acid bacteria has been used as biopreservatif becouse produce a number of antibacterial<br />substances are safety and has inhibitory activity against enteropatogenic bacteria. The aims of this study<br />were to screen of antibacterial compounds produced by Lactobacillus plantarum NS (9) and to produce<br />their antibacterial compounds. The research was devided into two stages. In the first stage was L. plantarum<br />NS (9) inoculated at 37°C, for 24 hours in semi-anaerobic conditions. The cell-free supertnatant was given<br />three treatment, ie not neutralized (A), neutralized (pH 7) (N), and precipitated with ammonium sulfate<br />50% (P). This three supernatant was assayed their antibacterial activity against E. coli, S. typhimurium<br />ATCC 14028, S. aureus, B. cereus and L. monocytogenes using the agar well diffusion method. In the second<br />stage, production of antibacterial compound was L. plantarum NS (9) inoculated at 37°C, for 24 hours<br />in semi-anaerobic conditions. The Dencity Optical, value pH, acid total and antibacterial activity were<br />measured every three hours during growth of bacteria. The results of the antibacterial screening showed<br />that L. plantarum NS (9) produced inhibitory zone againts the five indicator bacteria from a supernatant,<br />whereas N and P supernatant were not produced inhibitory zone. This result indicated that inhibition.</p><p>produced at 6 hours of incubation and were increased to simultaneously with increasing of bacteria growth.<br />The highest antibacterial activity against E. coli, B. cereus and L.monocytogenes were produced at the end<br />of the exponential growth phase (12 -15 hours incubation) while against S. aureus and S. typhimurium<br />ATCC 14028 at 21 and 24 hour of incubation, respectively. The antibacterial activity also was increased to<br />simultaniously with increasing of acid total (1.350 to 4.050%) and decreasing of pH value (6-4) during<br />growth of bacteria.<br /><br /></p>

scholarly journals Antibacterial Activity of Some Herbal Extracts for the Treatment of Typhoid Fever Sold in Okitipupa Town, Ondo State, Nigeria

2021 ◽  
Vol 6 (1) ◽  
pp. 86-90
Author(s):  
I. A. Adeyemo ◽  
◽  
C. O. Omolade
Keyword(s):  
Antibacterial Activity ◽  
Typhoid Fever ◽  
Test Organism ◽  
Water Extract ◽  
Salmonella Typhi ◽  
Hot Water ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Herbal Mixture ◽  
Plant Parts

The study investigated and compared the antimicrobial potency of aqueous (cold and hot) and ethanolic extracts of six plant parts used as herbal mixture against clinical isolate of Salmonella typhi in Okitipupa town. Extracts from six plant parts used as herbal mixture were tested for antibacterial activity against Salmonella typhi isolated from patients suffering from typhoid fever. The antibacterial activities of each of the extracts (ethanolic, cold and hot waters) were determined using agar diffusion method and the activities produced zone of inhibition ranging from 20mm-34mm. Ethanolic extract was found to be more potent being capable of exerting significant inhibitory activity on the test organism investigated with an inhibition zone of 34mm followed by the cold-water extract (28mm) while hot water extract gave the least (20mm). The phytochemical analysis showed the presence of saponins, tannins, flavonoids, phenols, glucosinolates and triterpenes in the extracts but total absence of proteins. The study confirms the medicinal value of herbal mixtures and justifies the claim of its local use in health remedies for typhoid fever caused by Salmonella typhi. There is the need therefore to develop effective combination of antimicrobial agents in purified form from higher plants and their parts for clinical trials. Keywords: Herbal mixture, antibacterial activity, typhoid fever, phytochemical analysis.

scholarly journals Antibacterial Activity of Benzyl Benzoate and Crotepoxide from Kaempferia rotunda L. Rhizome

2019 ◽  
Vol 20 (1) ◽  
pp. 9
Author(s):  
Hartiwi Diastuti ◽  
Mochammad Chasani ◽  
Suwandri Suwandri
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Activity Assay ◽  
Inhibitory Zone ◽  
Benzyl Benzoate ◽  
Disc Diffusion Method ◽  
Medium Level ◽  
And Staphylococcus Aureus

Benzyl benzoate and crotepoxide are the major components of Kaempferia rotunda L. rhizome. However, the bioactivity study of benzyl benzoate and crotepoxide as the antibacterial activity were still limited. Therefore, the antibacterial activity of benzyl benzoate and crotepoxide against four pathogenic bacteria, i.e., Escherichia coli ATCC 25922, Enterococcus aerogenes ATCC 13048, Bacillus cereus ATCC 6538 and Staphylococcus aureus ATCC 11778 were investigated. The isolation steps were include the extraction by maceration with acetone, then the acetone extract was partitioned with n-hexane:methanol (1:1) and ethyl acetate:water (1:1) respectively. The isolation by liquid vacuum chromatography followed by column chromatography was obtained benzyl benzoate from the n-hexane fraction and crotepoxide from ethyl acetate fraction. The molecular structure of isolated compounds was identified based on NMR (1D and 2D) spectroscopic data. The antibacterial activity assay of isolated compounds was carried out using the disc diffusion method. The antibacterial evaluation confirms that the benzyl benzoate and crotepoxide exhibits a medium level activity. Benzyl benzoate showed highest antibacterial activity against B. cereus at the MIC of 50 μg/mL and inhibitory zone of 5.9 mm, while the crotepoxide showed highest antibacterial activity against E. aerogenes at the MIC of 100 μg/mL with inhibitory zone 6.1 mm.

scholarly journals In vitro antimicrobial activity of plant active components against Pseudomonas lundensis and Listeria monocytogenes

2020 ◽  
Author(s):  
Khabat Noori Hussein ◽  
Tímea Molnár ◽  
Richard Pinter ◽  
Adrienn Toth ◽  
Emna Ayari ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Listeria Monocytogenes ◽  
Inhibitory Concentration ◽  
Agar Plate ◽  
Diffusion Method ◽  
Active Components ◽  
Plate Method ◽  
Gradient Plate ◽  
Strong Antimicrobial Activity

AbstractThis work aimed to study the antimicrobial activity of eight various components of plant origin on the growth of Pseudomonas lundensis and Listeria monocytogenes. Different in vitro methods were used: agar plate diffusion, micro atmosphere, agar hole diffusion, micro-dilution, and gradient-plate method. In the first agar plate assay, p-cymene and γ-terpinene did not inhibit the growth of the tested bacteria therefore they were not used in further experiments. Both α-pinene and limonene were only partially effective, but these were screened only for their partial inhibition. The other four components completely inhibited the growth of the tested bacteria. Using the agar-well diffusion method showed that carvacrol and thymol were found to be the most effective active components, thymol had minimum inhibitory concentration (MIC) at 1.563 mg/mL, however, in the case of carvacrol, MIC was 7.813 μL/mL. Additionally, eugenol and camphor show the same results but in higher concentrations. Gradient plate method was used to determine MIC values, in which it has been proved that carvacrol and thymol possess strong antimicrobial activity, no growth of tested bacteria was observed with carvacrol (100 μL/mL), while thymol exhibited MIC of 1.887 mg/mL against P. lundensis and 0.943 mg/mL needed to show complete inhibition of Listeria monocytogenes. Further experiments are needed to determine the optimum concentrations of the active components against P. lundensis and L. monocytogenes.

scholarly journals Skrining Aktivitas Antibakteri Ekstrak Etanol Daun Terhadap Salmonella Typhi Resisten Kloramfenikol

2017 ◽  
Vol 2 (02) ◽  
pp. 66
Author(s):  
Ika Trisharyanti
Keyword(s):  
Antibacterial Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Dilution Method ◽  
Tea Leaves ◽  
Disk Diffusion Method ◽  
Phaleria Macrocarpa

<em><span lang="EN-US">Typhoid fever is an infectious disease caused by Salmonella typhi, antibiotic chloramphenicol can be use to treatment, but S. typhi resistant with antibiotic choramphenicol so need to alternative treatment. The purpose of this study was to determine the antibacterial activity of ethanol extract of ten leaves against Salmonella typhi, and knowing the compounds contained in extracts of leaves that have the best antibacterial activity. The leaves was extracted with  ethanol 96% by maceration method. Screening antibacterial activity used disk diffusion method with 10% extract concentration. The best antibacterial activity was determined Minimum Inhibitory Concentration and Minimum Bactericidal Concentration used liquid dilution method, identification test of compounds with thin-layer chromatograpy (TLC), and bioautografi test. There were six extracts had antibacterial activity against S. Typhi, Phaleria macrocarpa leaves, Acalypha siamensis leaves, cherry leaves, clove leaves, tea leaves, and Eugenia polyantha leaves. Minimum Bactericidal Concentration from clove leaf extract was 2,5%. The results of identification by TLC compounds were alkaloids, phenolics, flavonoids, terpenoids, triterpenoids, and saponins. Based on bioautografi test,  phenolic can inhibit the growth of Salmonella typhi.</span></em>

scholarly journals Determination of minimum inhibitory concentration (MIC) of cloxacillin for selected isolates of methicillin-resistant Staphylococcus aureus (MRSA) with their antibiogram

1970 ◽  
Vol 6 (1) ◽  
pp. 121-126 ◽  
Author(s):  
MA Islam ◽  
MM Alam ◽  
ME Choudhury ◽  
N Kobayashi ◽  
MU Ahmed
Keyword(s):  
Staphylococcus Aureus ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Agar Plate ◽  
Susceptibility Test ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Methicillin Resistant ◽  
Antibiotic Susceptibility Test ◽  
Mrsa Strains

The minimum inhibitory concentration (MIC) represents the concentration of antimicrobial at which there is complete inhibition of growth of organism. In order to determine the MIC of cloxacillin, 10 MRSA were previously detected from 40 clinical isolates of Staphylococcus aureus by polymerase chain reaction (PCR). Agar plate dilution test was used to determine the MIC of cloxacillin. The clinical samples were collected from Mymensingh Medical College Hospital, Mymensingh. The study was done in the Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh from July 2006 to June 2007. The MIC of the cloxacillin for 5 MRSA strains were ≥32 (mg/ml), for 1 MRSA strain was ≥ 128(mg/ml) and for another 4 MRSA strains were above ≥128 (mg/ml). Antimicrobial susceptibility test of the isolated organisms were done by disc diffusion method. On antibiotic susceptibility test, MRSA strains showed 100% resistant against penicillin, oxacillin, cloxacillin and amoxycillin. Cent per cent susceptibility of MRSA was found against vancomycin, ciprofloxacin, erythromycin, fusidic acid and rifampicin. Key words: Minimum inhibitory concentration (MIC), antibiotic resistance, cloxacillin, methicillin-resistant Staphylococcus aureus (MRSA) DOI = 10.3329/bjvm.v6i1.1350 Bangl. J. Vet. Med. (2008). 6 (1): 121-126

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