In- vitroantioxidant and anti-obesity properties of Bauhinia variegata

2017 ◽  
Vol 2 (2) ◽  
pp. 128 ◽  
Author(s):  
M. M. Patil ◽  
T. Anand ◽  
N. Ilaiyaraja ◽  
Farhath Khanum
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Chloroform Extract ◽  
Polyphenol Content ◽  
Bioactive Properties ◽  
The Family ◽  
Bauhinia Variegata ◽  
And Oxidative Stress

<p><em>Bauhinia variegata</em> L (Synonyms: <em>Phanera variegata</em> Benth), commonly known as orchid tree or Kachnar belongs to the family of Leguminosae and found throughout India. All parts of the plants are used in the treatment of many ailments such as obesity, dyspepsia, bronchitis, leprosy, ulcer <em>etc.</em> The objective of the present study was to assess the i<em>n- vitro</em> antioxidant and anti-obesity properties of different solvent (hexane, chloroform, ethyl acetate, acetone, methanol and distilled water) extracts of <em>Bauhinia variegata</em>leaves. All the solvent extracts were found to contain different phytochemicals with bioactive properties at varying concentrations.The results of extraction showed that methanol was the suitable solvent for extraction of phytochemical as it recorded highest yield of 6.80%. The phytochemical assay results showed the highest polyphenol content in methanol extract (243 μg GAE/mg) and flavonoids content in chloroform extract (1.87 μg CE/g).The <em>in-vitro</em> antioxidant and anti obesity property was observed more in methanolic extract than the other extracts. The overall study suggests that <em>Bauhinia variegata</em>leaf extract could be a potential ingredient for the preparation of nutraceuticals to treat obesity and oxidative stress related complications. </p>

scholarly journals XANTHINE OXIDASE INHIBITORY ACTIVITY OF RHUS MYSORENSIS LEAVES

2018 ◽  
Vol 11 ◽  
Author(s):  
Vivekanandan K ◽  
Bhavya E ◽  
Stalin C
Keyword(s):  
Xanthine Oxidase ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Inhibiting Activity ◽  
Inhibition Concentration ◽  
Ic50 Value ◽  
Essential Enzyme ◽  
Preclinical And Clinical Studies

Objective: The objective of this study was to assess the in vitro xanthine oxidase (XO) inhibiting activity of the methanolic leaf extract of the Rhusmysorensis.Methods: Uric acid is synthesized in the presence of XO which is an essential enzyme. Various concentrations of the leaf extract were taken. XO inhibiting activity was spectrophotometrically assayed. By increasing absorbance at 295 nm, the degree of enzyme spontaneity was determined.Results: Inhibition concentration 50 (IC50) of the methanolic extract of R. mysorensis leaves was 45.24±0.34 μg/ml, compared with IC50 value of the standard allopurinol of 7.8±1.28 μg/ml.Conclusion: Methanol extract of R. mysorensis can be used to treat hyperuricemia and gout after proper preclinical and clinical studies.

scholarly journals Broad-Spectrum Antimicrobial, Antioxidant, and Anticancer Studies of Leaf Extract of Simarouba glauca DC In Vitro

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 59
Author(s):  
Shanmuga Priya Ramasamy ◽  
Anitha Rajendran ◽  
Muthukrishnan Pallikondaperumal ◽  
Priya Sundararajan ◽  
Fohad Mabood Husain ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Cancer Cell Line ◽  
Ethanol Extract ◽  
Bioactive Compound ◽  
Potential Candidate ◽  
Simarouba Glauca

The current study aimed to screen the preliminary phytochemicals in the leaf extract of the medicinal plant Simarouba glauca and to analyze its potential antimicrobial, antioxidant and anticancer properties. The phytochemical profile of the methanol extract was analyzed, and bioactive compounds were identified using chromatography, FTIR and GCMS. Antimicrobial activity and Minimum Inhibitory Concentration (MIC) were determined against 14 bacterial and 6 fungal strains. Moreover, the synergistic effect of a plant extract with commercially available antibiotics was also evaluated using the checkerboard method. The ethanolic and methanolic extracts showed exclusive activity against S. aureus and profound activity against E. coli and S. marcescens. Upon comparing breakpoints, methanolic extract demonstrated higher antimicrobial activity with a MIC value of 3.2 mg/mL against the test pathogens. Furthermore, the extracts demonstrated potential antioxidant activity; methanol extract had higher antioxidant potential compared to the ethanol extract. The major proactive bioactive compound with maximum antioxidant capacity was observed to be terpenoids. The methanol extract of S. glauca showed significant cytotoxicity against the MCF-7 breast cancer cell line with an IC50 value of 16.12 µg/mL. The overall results of our work provide significant evidence for the usage of methanolic extract of S. glauca as an efficient ethnomedicinal agent and a potential candidate for relieving many human ailments.

scholarly journals IN VITRO CYTOTOXIC ACTIVITY OF ISOLATED COMPOUNDS FROM VIBURNUM PUNCTATUM BUCH-HAM EX D. DON

2016 ◽  
Vol 9 (1) ◽  
pp. 85
Author(s):  
A. Renjith Alex ◽  
K. Ilango
Keyword(s):  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Chloroform Methanol ◽  
Human Colon Cancer Cells

Objective: The main aim of the study was to screen the isolated compounds of Viburnum Punctatum for its in vitro anticancer activity and its percentage viability against HCT 15 (Human Colon Cancer Cells) Cell lines.Methods: Pet ether, Chloroform, Methanol and Aqueous extracts was prepared and assayed for the presence of phytochemicals. Two compounds were isolated from the methanol extract of Viburnum Punctatum by column chromatography such as ME1 (Quercetin) and ME2 (Kaemferol-3-glycoside) characterised by UV, IR, MS, 1H NMR and 13C NMR. The above isolated compounds were subjected to in vitro anticancer activity on HCT 15 cell lines was evaluated by Micro culture Tetrazolium (MTT) assay.Results: ME1 showed significant cytotoxic activity than the ME2 on HCT 15 cells with a percentage viability of 54.60 and 67.18 in the concentration of 10µg/ml and 50µg/ml respectively.Conclusion: On the basis of obtained results, ME1 and ME2 isolated from a methanolic extract of Viburnum Punctatum represent a new group of cytotoxic against HCT 15 Cell lines.

scholarly journals Photoprotection and Photostability of a New Lignin-Gelatin-Baccharis antioquensis-Based Hybrid Biomaterial

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1904
Author(s):  
Juan C. Mejía-Giraldo ◽  
Juan C. Scaiano ◽  
Cecilia Gallardo-Cabrera ◽  
Miguel A. Puertas-Mejía
Keyword(s):  
Phenolic Content ◽  
Methanolic Extract ◽  
Polyphenol Content ◽  
Protection Factor ◽  
Dry Extract ◽  
Natural Extract ◽  
Natural Extracts ◽  
Electrostatic Stability ◽  
Better Than

The aim of this study was to develop a new hybrid biomaterial that could photo-stabilize and improve the photoprotective capacity of a Baccharis antioquensis extract. Different combinations of lignin/gelatin/natural extract were applied to prepare hybrid biomaterial nanoparticles (NPs), which were then incorporated into an emulsion. The in vitro photoprotection and photostability were evaluated. The methanolic extract showed high phenolic content (646.4 ± 9.5 mg GAE/g dry extract) and a DPPH radical assay revealed that the antiradical capacity of the extract (0.13 to 0.05 g extract/mmol DPPH) was even better than that of BHT. The particle size of the hybrid biomaterial ranged from 100 to 255 nm; a polydispersity index (PdI) between 0.416 and 0.788 is suitable for topical use in dermocosmetic products. The loading capacity of the extract ranged from 27.0 to 44.5%, and the nanoparticles (NPs) showed electrostatic stability in accordance with the zeta potential value. We found that the formulation based on lignin: extract (1:1 ratio) and gelatin: lignin: extract (0.5:0.5:1 ratio) demonstrated photoprotection qualities with a sun protection factor (SPF) ranging from 9.4 to 22.6. In addition, all the hybrid NP-formulations were time-stable with %SPFeff and %UVAPFeff greater than 80% after exposure to 2 h of radiation. These results suggest that the hybrid biopolymer-natural extract improved the photoprotection and photostability properties, as well as the antiradical capacity, of the B. antioquensis extract, and may be useful for trapping high polyphenol content from natural extracts, with potential application in cosmeceutical formulations.

scholarly journals Phytotoxicity of plant extracts of Vismia japurensis cultivated in vivo and in vitro

2022 ◽  
Vol 82 ◽  
Author(s):  
L. M. Lima ◽  
L. S. Pedroza ◽  
M. I. C. Osório ◽  
J. C. Souza ◽  
C. V. Nunez
Keyword(s):  
Lactuca Sativa ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Biotechnological Potential ◽  
1H Nuclear Magnetic Resonance ◽  
Sandwich Method ◽  
Aromatic Region ◽  
Layer Chromatography

Abstract Plants that produce secondary metabolites with allelopathic activity or phytotoxicity can be biotechnologically important, serving as sources of allelochemicals, and thus contributing to the agroindustrial sector. Vismia japurensis (Hypericaceae) is an Amazonian species that grows in clumps called vismiais, from which most other plants are absent. Accordingly, the objective of this study was to identify possible phytotoxicity effects of hexane and methanol extracts of Vismia japurensis leaves and branches in vivo and from seedlings grown in vitro on Lactuca sativa. In addition, fresh and dry leaves were assayed by the sandwich method in order to determine their ability to release allelochemicals. The hexanic extract from in vitro seedlings reduced germination by 10%, while the methanol extract produced a 16% reduction in germination speed. Root growth of Lactuca sativa was inhibited by 64.7% when subjected to hexane leaf extract, by 39.3% under the influence of hexane branch extract, and by 96.09% for in vitro seedling hexanic extract. When analysed by thin layer chromatography and 1H nuclear magnetic resonance, extracts showed evidence of terpenes, anthraquinones and flavonoids, with greater intensity of signals in the aromatic region of in vitro seedling hexanic extract. Clearly, Vismia japurensis has a high biotechnological potential in terms of the production of substances of low polarity with capacity to interfere in plant development.

scholarly journals Phytochemical screening and in-vitro antibacterial and DPPH free radical scavenging activities of methanol extract of root of Combretum album Pers.

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Functional Groups ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Chloroform Fraction ◽  
Active Ingredients ◽  
Ic50 Value ◽  
Ft Ir ◽  
Ir Analysis

The objective of the study was to investigate in-vitro antibacterial and antioxidant activities of methanolic extract of Combretum album Pers. root. In brief antibacterial efficacies of methanol extract and its petroleum ether fraction (MePET), chloroform fraction (MeCH), ethyl acetate fraction (MeEA) and water fraction (MeAQ) were determined by agar well diffusion assay along with Minimum Inhibitory Concentrations (MICs). In-vitro antioxidant efficacies were evaluated by DPPH radical scavenging method. Preliminary phytochemical assay, Fourier Transform Infrared Spectroscopy and Gas Chromatography-Mass Spectroscopy analyses were employed to detect the plausible active ingredients. Methanol extract showed broad spectrum antibacterial activity having highest inhibition zone against Staphylococcus aureus MTCC 2940 (21.67±0.58 mm) and MICs ranged from100 µg/ml to 250 µg/ml. The scavenging activity of methanol extract of root of C. album was concentration dependant and IC50 value was 136.08 µg/ml. The lowest MIC (5 µg/ml) was noted with MeCH against B. subtilis (MTCC 441). MeCH showed highest antioxidant activity with an IC50 value of 12.98 ?g/mL and MePET, MeEA, MeAQ and Ascorbic acid presented antioxidant potential with IC50 values of 16.10, 15.07, 17.44 and 13.40??g/mL respectively. Preliminary phytochemical tests and FT-IR analysis revealed presence of various phytochemicals and functional groups like hydroxyl, carboxylic acids, amidines, amines, aromatics and esters. Three compounds were elucidated from bioactive TLC fraction of MeCH in GC-MS analysis supported by presence of various functional groups in MeCH as detected in FT-IR analysis. It is concluded that methanolic extract of C. album root is a potential antibacterial and antioxidant agent where presence of N-[5-[4-pyridinyl)-1H-1,2,4-triazol-3-yl]-benzamide (compound 1), pyruvic acid (compound 2) and methyloacetone (compound 3) are responsible as possible active ingredients.

scholarly journals ANTIOXIDANT PROPERTIES OF METHANOLIC EXTRACT OF BRYPHYLLUM PINNATUM ON ALBINO WISTAR RATS

2021 ◽  
Vol 9 (12) ◽  
pp. 270-282
Author(s):  
Solomon Chimaraoke Egeonu ◽  
◽  
Akataobi Kelechi Uzoma ◽  
Eke Ugorji Iheanacho ◽  
◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Succulent Plant ◽  
Activity Test ◽  
The Family ◽  
Treatment Of Hypertension ◽  
Group 2 ◽  
Test Result ◽  
Group 1

Bryophyllumpinnatum, which belong to the family Crassulceae, is a succulent plant native to Madagascar, which is a popular houseplant and has become naturalized in tropical and subtropical areas. It has been in traditional medicine for the treatment of hypertension and kidney stones. Despite these acclaimed efficacies of its use in trado-medical practices, the effects on body organs are not well understood. The aim of this study was to evaluate antioxidant properties of Bryophyllumpinnatum. Fifteen adult Sprague dowley rats were use in this study split into three groups (group 1, 2 and 3). Group 1 animals served as the control, while group 2 and 3 served as the experimental groups. Acute toxicity, and antioxidants. The result of this study found LD50 to be above 5000mg/kg. In vitro antioxidant test found the presence of alkaloids, saponins, thiamin, riboflavin etc. Antioxidant activity test result found an increase in GSH, GPx, a decrease in MDA and a stable CAT. In conclusion, we recommend its uses in human, but at a regulated doses because of its efficacy as stated in this study.

scholarly journals Inhibition of LDL-oxidation and antioxidant properties related to polyphenol content of hydrophilic fractions from seaweed Halimeda Incrassata (Ellis) Lamouroux

2012 ◽  
Vol 48 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Ariana Costa-Mugica ◽  
Ana Elsa Batista- Gonzalez ◽  
Diadelis Mondejar ◽  
Yosdel Soto-López ◽  
Victor Brito-Navarro ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Ldl Oxidation ◽  
Dpph Radical ◽  
Polyphenol Content ◽  
Reducing Activity ◽  
Dpph Radical Scavenging ◽  
And Oxidative Stress

LDL oxidation and oxidative stress are closely related to atherosclerosis. Therefore, natural antioxidants have been studied as promising candidates. In the present study, the LDL oxidation inhibition activity of bioactive compounds from Halimeda incrassata seaweed. associated to antioxidant capacity, was evaluated in vitro. Experimental work was conducted with lyophilized aqueous extract and phenolic-rich fractions of the seaweed and their effect on LDL oxidation was evaluated using heparin-precipitated LDL (hep-LDL) with exposure to Cu2+ ions and AAPH as the free radical generator. H. incrassata had a protective effect for hep-LDL in both systems and the presence of phenolic compounds contributed to the activity where phenolic-rich fractions showed significant capacity for inhibition of oxidation mediated by Cu2+ ions. The observed effect could be related to the antioxidant potential of polar fractions evidenced by reducing activity and DPPH• radical scavenging. The results obtained in vitro further support the antioxidant and LDL oxidation inhibition properties of H. incrassata and further knowledge toward future phytotherapeutic application of the seaweed.

scholarly journals In-vitro Antivenom Potential of Solanum dasyphyllum Methanolic Leaf and Fruit Extracts against Naja nigricollis Venom

2020 ◽  
pp. 38-45
Author(s):  
Adewunmi Rofiat Funmilola ◽  
Gidado Abubakar ◽  
Zanna Hassan
Keyword(s):  
Medicinal Plants ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Fruit Extract ◽  
Southwestern Part ◽  
Fruit Extracts ◽  
Snake Envenomation ◽  
The Family ◽  
Inhibitory Potential

Solanum dasyphyllum belongs to the family of plants called Solanaceae, it is commonly called "Africa eggplant" and one of the medicinal plants used in the treatment of snake envenomation in the southwestern part of Nigeria, but investigation concerning its anti-venom activity has not been established. The present study evaluates the in-vitroenzyme inhibition potential of S.dasyphyllum leaf and fruit extracts against Naja nigricollis (Black-necked spitting cobra) venom. The inhibitory potential of S. dasyphyllum leaf and fruit on proteases, acetylcholinesterase, phospholipase A2 and hyaluronidase enzymes present in the snake venom was evaluated. The methanolic leaf and fruit extracts of S. dasyphyllum inhibited the activity of all enzymes evaluated, however, the leaf extract exhibited better enzyme inhibitory effect on N. nigricollis venom when compared with the fruit. This could be due to the presence of various phytochemicals in leaf and fruit extract.  This result substantiates the ethnomedicinal usage of S. dasyphyllum and would help to develop potent antidote therapy against N. nigricollis envenomation.

In vitro Physicochemical, Phytochemical, Antimicrobial and Antidiabetic studies on Mucuna pruriens (Linn.) DC seeds

2016 ◽  
Vol 5 (06) ◽  
pp. 4650 ◽  
Author(s):  
Srilakshmi Kusuma* ◽  
Aniel Kumar O. ◽  
Lakshmi Narayana K. ◽  
Sudhakar Pola ◽  
Venkata Reddy K.
Keyword(s):  
Developing Countries ◽  
Methanol Extract ◽  
Mucuna Pruriens ◽  
Bioactive Properties ◽  
Treatment Of Diabetes ◽  
Relative Deficiency ◽  
Alpha Glucosidase ◽  
Crude Methanol Extract ◽  
Seed Extracts

Diabetes is one of the most common endocrine diseases characterized by hyperglycemia due to absolute or relative deficiency of insulin which is currently affecting the citizens of both developed and developing countries. According to Williams textbook of endocrinology in 2013 it was estimated that over 382 million people throughout the world had diabetes. Plants have long been used for the treatment of diabetes, particularly in developing countries where most people have limited resources and do not have access to modern treatment. The presented study is aimed to evaluate the Mucuna pruriens seed extracts for its in vitro physiochemical, phytochemical, antioxidant and anti-diabetic studies. From the studies different solvent extracts of hexane and chloroform showed little or no activity on all assays performed whereas methanol extract of Mucuna pruriens showed significant bio properties. The preliminary studies of this plant crude methanol extract exhibited maximum compounds, hence the methanol extracts have under taken for its alpha amylase and alpha glucosidase inhibition activity. On the basis of the results obtained in the present investigation, it can be concluded that methanol extract of Mucuna pruriens had significant bioactive properties and may provide a support to use of the plant in traditional medicine for the management of diabetes.

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