scholarly journals Toxicity Assessment of Biologically Degraded Product of Textile Dye Acid Red G

2019 ◽  
Vol 4 (4) ◽  
pp. 236-243
Author(s):  
Priti Faldu ◽  
Vishal Kothari ◽  
Charmy Kothari ◽  
Jalpa Rank ◽  
Ankit Hinsu ◽  
...  
Keyword(s):  
Azo Dyes ◽  
Environmental Concern ◽  
Bacterial Species ◽  
Amino Nitrogen ◽  
Positive Control ◽  
Toxicity Assessment ◽  
Textile Dye ◽  
Dye Pollutants ◽  
Chromosome Aberration Assay ◽  
Acid Red G

Azo dyes are of environmental concern due to their recalcitrant nature. Several azo dyes and their decolorized and degraded products exert toxic and mutagenic effects on the flora and fauna. The toxic properties of these azo dyes are due to nature and position of the substitution with respect to the aromatic rings and amino nitrogen atoms. Several studies have thus far been emphasized on biodegradation of azo dye pollutants, though role of their biodegraded product is rarely studied. Given a lack of this understanding, we have analyzed the effects of degraded products of a di-azo textile dye Acid Red G by newly isolated bacterial species, Pseudomonas aeruginosa PFK10 and Brevibacillus choshinensis PFK11. The genotoxicity and cytotoxicity of Acid Red G and their degraded products were tested on HeLa cell line and Human lymphocyte cell, respectively. The data of MTT assay has been shown that activity of degraded products of the Acid Red G were comparable to their parent dye. But chromosome aberration assay and sister chromatid exchange assay did not show any significant changes in chromosomes as compared to positive control mitomicine.

scholarly journals Isolation and Screening of Water Microbes for Decolourisation of Textile Dye Waste

2016 ◽  
Vol 11 (1) ◽  
pp. 296-300
Author(s):  
J. K Singh ◽  
R Ranjan ◽  
Pranay Pankaj
Keyword(s):  
Waste Water ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Azo Dyes ◽  
Bacterial Species ◽  
Textile Dye ◽  
Human Beings ◽  
Degrading Bacteria ◽  
Vis Spectroscopy ◽  
Pre Treatment

Azo dyes are widely used in textile industry. Unused dyes, consisting mainly non biodegradable released along with waste water streams without any proper pre-treatment which cause nuisance for environment and accumulate in flora as well as fauna. These also exhibit allergic, carcinogenic and mutagenic properties for human beings. Isolation and screening of azo dye degrading bacteria are economic in biodegradation and detoxification. In the present study, 200 waste water samples were collected from dye-contaminated sites of textile industries and bacterial species such as Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida were isolated and identified. Evaluation of decolorizing properties of these bacteriae were done by UV-Vis spectroscopy (Amax 596 nm) in different concentrations using different carbon sources such as Hans’s medium and GYP medium. Maximum decolourisation of 0.1% azo dyes were recorded to be 89.0%, 91% and 86% in Hans medium containing charcoal source by Bacillus subtilis, Pseudomonas aeruginosa and Psuedomonas putida respectively at 24 hrs. These bacterial isolates may be utilized in large scale for pre-treatment for ecological balance by avoiding water pollution.

scholarly journals Screening Of Antibacterial Activity Of Nepeta Ciliaris Benth. Against Respiratory Tract Pathogens

1970 ◽  
Vol 8 (1) ◽  
pp. 100-103 ◽  
Author(s):  
Gautam Shiv Shankar ◽  
M Navneet ◽  
Kumar Sanjay ◽  
M Prabhat
Keyword(s):  
Respiratory Tract ◽  
Antimicrobial Agents ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Bacterial Species ◽  
Positive Control ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Aerial Parts ◽  
Antibacterial Potential

The aim of present study was to evaluate the antibacterial potential of various extracts (petroleum ether, acetone, methanol and aqueous) of Nepeta ciliaris against selected respiratory tract pathogens. The extracts from the aerial parts of N. ciliaris at concentration of 200 mg/ml were screened against three gram-positive (Staphylococcus aureus MTCC 1144, Streptococcus pneumoniae MTCC 655 and Streptococcus pyogenes MTCC 442) and one gram-negative (Pseudomonas aeruginosa MTCC 2474) bacterial pathogens. The agar well diffusion method was adopted to examine antibacterial and minimum inhibitory concentration (MIC) values of most effective extracts against the susceptible bacteria. Erythromycin was used as positive control to determine the sensitivity of the strains. Out of the four bacterial species tested, S. pneumoniae was the most susceptible. The acetone extract exhibited maximum activity against all the tested microorganisms while methanol extract showed activity against P. aeruginosa. The MIC values ranged from 40 to 50 mg/ml for all the organisms. The N. ciliaris is potentially a good source of antimicrobial agents. DOI: http://dx.doi.org/10.3126/kuset.v8i1.6049 KUSET 2012; 8(1): 100-103

scholarly journals Antimicrobial Activity of an Amnion-Chorion Membrane to Oral Microbes

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Haroon Ashraf ◽  
Kerri Font ◽  
Charles Powell ◽  
Michael Schurr
Keyword(s):  
Bacterial Species ◽  
Healing Process ◽  
Antimicrobial Properties ◽  
Positive Control ◽  
Negative Control ◽  
Collagen Membrane ◽  
Wound Healing Process ◽  
Bacterial Strains ◽  
Oral Microbes ◽  
Negative Controls

Objective. The aim of this study was to evaluate wound biomodification by assessing antimicrobial properties present within a human-derived composite amnion-chorion membrane (ACM). Methods. Membranes analyzed were the human-derived ACM BioXclude™ and the porcine-derived collagen membrane Bio-Gide®. Paper discs with and without tetracycline served as positive and negative controls, respectively. The same number of colony-forming units per milliliter for each bacterial species (Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Streptococcus oralis) was inoculated on each of the discs. Discs from each group were removed at 12 and 24 hours and sonicated to remove the bacteria off the membranes. A serial dilution was performed to quantify bacterial growth. Results. The ACM inhibited growth at all time points, with all bacterial strains, identical to the negative control tetracycline discs. The collagen membrane and positive controls did not inhibit growth of any of the bacterial species throughout the 24-hour study period. P<0.05 for microbial growth on ACM or negative control vs. either collagen membrane or positive control. Conclusion. ACM was proven to be as bactericidal as paper discs inoculated with tetracycline at its minimum bactericidal concentration. The ACM bactericidal property may be beneficial in the early wound healing process.

scholarly journals STUDI PENURUNAN ZAT WARNA ACID ORANGE 7 DENGAN PROSES OKSIDASI MENGGUNAKAN FERRAT (FeO4)2-

Molekul ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. 30
Author(s):  
Dian Windy Dwiasi ◽  
Suyata Suyata
Keyword(s):  
Textile Wastewater ◽  
Molar Ratio ◽  
Textile Dye ◽  
Acid Orange 7 ◽  
Acid Orange ◽  
Oxidation Processes ◽  
Dye Pollutants ◽  
Receiving Waters ◽  
Physical And Chemical ◽  
Oxidation Degradation

Colour removal from textile wastewater has been a matter of considerable interest during the last two decades, not only because of the potential toxicity of certain dyes but often due to their visibility in receiving waters and to their low biodegradability. Due to the limited success of some physical and chemical techniques for the treatment of dye effluents it is necessary to develop destructive systems leading to complete mineralization or, at least, to less harmful or easy-to-treat compounds. Oxidation processes using ferrate (FeO4)2- have been found to be very effective in the degradation of dye pollutants and for the treatment of waste waters from the textile industry.Acid Orange 7 (AO7) commonly used as a textile dye and could be degraded by ferrate oxidation processes. In the oxidation degradation of dye by ferrate process, effect of some parameters such as time, pH, and molar ratio of dye was examined at experimental condition. AO7 removal by this process was calculated to be equal to 12 minute at experimental condition. Ferrate can oxidize acid orange 7 effectively at optimum pH of 9.8, with the molar ratio of ferrate : acid orange 7 at 4:1. The percentages of acid orange 7 degradation reached to 98.9%.

scholarly journals Characterization and toxicity effect of leachate from municipality landfill

2021 ◽  
Author(s):  
Elias Jigar Sisay ◽  
Shivraj Sahai ◽  
Teame Tesfay
Keyword(s):  
Seed Germination ◽  
Environmental Concern ◽  
Chemical Characterization ◽  
Soil Surface ◽  
Toxicity Assessment ◽  
Toxicity Effect ◽  
Msw Landfill ◽  
Physico Chemical ◽  
Nutritional Property ◽  
Pea Seed

Abstract Leachate from Municipal Solid Waste (MSW) landfill has long known to be an environmental concern worldwide. The composition of landfill leachate may contain metals, ammonia, organics, other toxicants and carcinogens, having major environmental concern, with implications for plant, animal and human health. The pollution of soil, surface and ground water is also some of the major immediate concern related to leachate. This problem is growing at alarming rate in some of the developing countries including Ethiopia, and Adigrat happens to one such fast growing small city Ethiopia. However, in the absence of any significant relevant study for Adigrat City MSW leachate, the present work was undertaken to study the physico-chemical characterization of leachate from the Adigrat MSW leachate and investigate its toxicity effect on pea seed germination (Pisum sativum). The characterization was performed on the leachate from Adigrat MSW landfill. Subsequently, the leachate toxicity assessment on pea seed germination was also carried out. The result shows presence of several unacceptable components in the leachate that in some instance was detected at such levels that may pose environmental risk. It was also found that the low pH may add corrosiveness to any contaminated environmental components. Anions (like Cl−) and heavy metals (like Pb, Zn and Cd) detected in the leachate may also be concern for any possible environmental exposure. Additionally, the pea seed germination experiment suggests existence of such components in the leachate that possibly mask its nutritional property during germination.

scholarly journals Physicochemical Analysis and Microbial Diversity of Yamuna Water and Industrial Effluents

2014 ◽  
Vol 2 (2) ◽  
pp. 199-205 ◽  
Author(s):  
Poonam Gupta ◽  
Monika Asthana ◽  
Avnish Kumar ◽  
Siddhartha Barun
Keyword(s):  
Ph Value ◽  
Environmental Concern ◽  
Bacterial Species ◽  
Industrial Effluents ◽  
Chloride Content ◽  
Physicochemical Analysis ◽  
Tannery Effluent ◽  
Tannery Effluents ◽  
Textile Effluents ◽  
Standard Values

Pollution has arisen as a serious environmental concern to the present world after industrialization of human societies. It has severely affected our air, soil and water sources. Looking to its global, national, regional and local dimensions, it is now imperative to check it at each and every level. In the present study, 8 samples (3 Yamuna water samples, 3 tannery effluent samples and 2 textile effluent samples), were collected from different sites of Yamuna and exit points of textile and tannery Industries. Water and effluent samples were analysed for various physicochemical parameters (pH, TDS, hardness, chloride and BOD) using conventional methods. Afterwards these samples were utilized for isolation of the native bacterial species. All the samples were showing higher than the standard values for TDS (500mg/l), hardness (80-100 mg/l), chloride (250mg/l) and BOD (30mg/l). It was observed that the tannery effluents were showing maximum TDS values(1190-1240mg/l), followed by textile effluents (1190 and 1210mg/l) and Yamuna water (530-1180mg/l).Similarly, in case of chloride content, highest concentration range(828.8-1598mg/l)was shown by tannery effluents.  pH value was nearly neutral for Yamuna water, slightly  acidic in case of textile effluents and more acidic for tannery samples. Highest range of hardness values were observed for the tannery effluents (860-880mg/l) followed by textile effluents (760 and 860mg/l). The BOD values were nearly similar for all the samples with maximum values being observed for tannery effluents (42-48 mg/l). Thus it can be inferred that all the samples were highly polluted and need to be treated by suitable methods. There were 11 cultures purified, that could be employed in bioremediation purposes.DOI: http://dx.doi.org/10.3126/ijasbt.v2i2.10352Int J Appl Sci Biotechnol, Vol. 2(2): 199-205 

Advances in Bioremediation for Removal of Toxic Dye from Different Streams of Wastewater

2016 ◽  
pp. 266-278 ◽  
Author(s):  
Priya Banerjee ◽  
Aniruddha Mukhopadhayay ◽  
Papita Das
Keyword(s):  
Azo Dyes ◽  
Environmental Concern ◽  
Dye Degradation ◽  
Maximum Efficiency ◽  
Bacterial Cells ◽  
Existing Problems ◽  
Textile Printing ◽  
Enhanced Bioremediation ◽  
Living Organisms ◽  
Immobilization Techniques

Azo dyes are used in abundance in several industries like textile, printing, paper, plastic, cosmetics, paints, etc. Extensive discharge of such dyes in adjacent water bodies has raised much environmental concern. Azo dyes are toxic to living organisms and their genotoxic and carcinogenic potentials are intensified on being released as mixtures. In the recent years, various microorganisms have been isolated and reported to possess tremendous potential for efficient dye degradation. However, the process of bioremediation is highly controlled by experimental factors like effluent pH, temperature and concentration of dyes in solution. Therefore, appropriate optimization of these factors is to be determined in order to ensure maximum efficiency of this process. This review highlights application of immobilization techniques of bacterial cells for achievement of successful biodegradation. In this study, the existing problems of dye pollution and possible improvisations for obtaining enhanced bioremediation of dyes have also been discussed.

Determination of Three Chromium Textile Azo Dyes in Wastewater by SPE-LC-ESI(–)-MS/MS

2018 ◽  
Vol 101 (5) ◽  
pp. 1422-1428
Author(s):  
Florina-Maria Copaciu ◽  
Dorina Simedru ◽  
Maria-Virginia Coman
Keyword(s):  
Azo Dyes ◽  
Solid Phase ◽  
Treatment Plant ◽  
Textile Dye ◽  
Esi Ms ◽  
Dyeing Process ◽  
The Matrix ◽  
Wastewater Samples ◽  
Textile Factory

Abstract In the present work, a procedure to determine three textile azo dyes, chromium-complexes [Nylosan Dark Brown (NDB), Lanasyn Dark Brown (LDB), and Lanasyn Red (LR)], from wastewater using solid-phase extraction (SPE) followed by LC-electrospray ionization negative mode tandem mass spectrometry (LC-ESI(–)-MS/MS) has been developed. The extraction/concentration and recovery degree of these dyes from liquid matrices were done on Strata WAX/NH2 cartridges. The chromatographic separation was performed using a Luna C18 (2) 100Å column by isocratic elution with a methanol–acetonitrile–water (0.2% formic acid and 2 mM ammonium formate) mixture. The linearity, the LOD, and the LOQ were determined for each textile dye. The accuracy, the precision (intra- and inter-day), and the matrix effect were also performed for the validation of the developed procedure. These chromium-complex azo dyes often used in the dyeing process by a textile factory in Romania were monitored in the influent and effluent wastewater from a treatment plant situated in its area. Applying the developed SPE-LC-ESI(–)-MS/MS procedure, the following textile dyes were detected (ng/L) in the collected wastewater samples during a 24-h period: NDB 150.1, LDB 200.6, and LR 89.0–244.0 in influents and NDB 22.8, LDB 78.6, and LR 74.0 in effluents.

scholarly journals A comprehensive study on adsorption behavior of some azo dyes from aqueous solution onto different adsorbents

2017 ◽  
Vol 76 (2) ◽  
pp. 478-489 ◽  
Author(s):  
Nihan Kaya
Keyword(s):  
Aqueous Solution ◽  
Activated Carbon ◽  
Azo Dyes ◽  
Binding Capacity ◽  
Agricultural Wastes ◽  
Walnut Shell ◽  
Isotherm Models ◽  
Adsorption Parameters ◽  
Dye Pollutants ◽  
Comprehensive Study

One of the major environmental problems is the existence of dye materials in water sources. This pollutant must be removed from water by appropriate methods. Although most of these methods are efficient for the treatment of waters contaminated with dye pollutants, they are very costly and commercially unattractive. Adsorption is one of the most popular methods for the removal of dye pollutants from water especially if the sorbent is inexpensive. Sorbents are classified as natural sorbents, commercial sorbents, and the sorbents obtained from industrial and agricultural wastes. Although commercial activated carbon is widely used in wastewater treatment applications, it is very expensive. In this study, usability of inexpensive materials – sepiolite as a natural adsorbent, and walnut shell and hazelnut shell as agricultural wastes – was investigated instead of commercial activated carbon to remove some azo dyes (methyl red, methyl orange and methyl yellow) from aqueous solution. Batch experiments were carried out to determine the effect of different adsorption parameters such as pH, initial dye concentration, contact time, adsorbent dose and temperature. The equilibrium of adsorptions was modeled using Langmuir and Freundlich isotherm models. This comprehensive study showed that these alternative adsorbents had sufficient binding capacity to remove these azo dyes from water.

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