scholarly journals The Use of Torbangun Leaf Powder (Coleus amboinicus L) in Increasing Prolactin in Breastfeedings Wistar Rats and Bodyweight of the Litters

2021 ◽  
Vol 3 (1) ◽  
pp. 26-33
Author(s):  
Stella T Hasianna ◽  
July Ivone ◽  
Sifa D Shinta ◽  
R Zahra Nadhira
Keyword(s):  
Body Weight ◽  
Breast Milk ◽  
Wistar Rats ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
Breast Milk Production ◽  
Significant Difference ◽  
Leaf Powder ◽  
Coleus Amboinicus

Research on local herbs that have the effect of increasing breast milk production isneeded to help mothers with insufficient breastmilk. This research was conducted to determinethe effect of torbangun (Coleus amboinicus L) leaf powder in increasing prolactin inbreastfeeding Wistar rats and the bodyweight of the litters. Thirty breastfeeding Wistar rats weredivided into 5 groups with 5 litters each. The treatments given were 108 mg, 216 mg, and 432 mgtorbangun leaf powder (TLP), with domperidone as the positive control, and CMC 10% as thenegative control. The treatment was given 3 times/day for 14 days. Tukey's HSD means testshowed a significant difference in prolactin levels between the negative control (NC), TLP1, andTLP2 groups and the TLP3 and positive control (PC) groups with all of them having p = 0.000.There was no difference in the increase in body weight of litters between treatment groups (p =0.866). In conclusion, torbangun leaves increase the prolactin levels in breastfeeding rats buthave no difference in the increase in body weight of litters between treatment groups. Keywords: torbangun leaves; prolactin; bodyweight; breastfeeding

scholarly journals 14. The Potency of Sernai (Wedelia biflora) Leaf N-hexan Extract as Analgesic Compared to Ibuprofen on Mice (Mus musculus)

2018 ◽  
Vol 12 (2) ◽  
pp. 84-90
Author(s):  
Zulkarnaini Zulkarnaini ◽  
Rinidar Rinidar ◽  
Rosmaidar Rosmaidar ◽  
T. Armansyah TR ◽  
Abdul Harris ◽  
...  
Keyword(s):  
Body Weight ◽  
Positive Control ◽  
Negative Control ◽  
Male Mice ◽  
Hot Plate ◽  
Plate Method ◽  
Treatment Groups ◽  
Significant Difference ◽  
Plot Design ◽  
Leaf N

This study aimed to determine the analgesic potential of sernai leaves n-Hexan extract on 24 male mice, aged 2-3 month with 20-25 g weighed. This study used split-plot design consisted of 6 groups. P0 as negative control was given aquades and P1 as positive control was given ibuprofen (10 mg/kg Body Weight). Treatment groups including P2, P3, P4, and P5 were given n-Hexan extract of sernai leaves with dosage of 15, 30, 45, and 60 mg/kgBW, respectively. Pain assessment was conducted using Hot Plate method. Observation of mice behaviour including licking it's front and back leg, or by jumping. This study showed there was significant difference (P0,05) between dosage and time. Duncan’s test showed P1 was significantly different (P0,05) from P2, but P3 and P5 were not significantly different from P1. P3 and P4 were significantly different (P0,05) from P2 and not significantly different from P1, while P5 was not significantly different from P3 and significantly different from P1. We can conclude that dosage of 30 and 45 mg/kgBW of n-Hexan extract of sernai leaves had analgesic potential on mice, and 60 mg/kgBW was comparable to ibuprofen as pain relieve in mice

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals 2. Anti-Bacterial Activity Of N-Hexane Extract Of Malacca Leave (Phyllanthus emblica) On Mice (Mus musculus) Inoculated By Staphylococcus epidermidis In Vivo

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Nada Sarah Syahputri ◽  
Nuzul Asmilia ◽  
Rinidar Rinidar ◽  
Amalia Sutriana ◽  
Fakhrurrazi Fakhrurrazi ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Positive Control ◽  
Negative Control ◽  
Hexane Extract ◽  
Phyllanthus Emblica ◽  
Treatment Groups ◽  
Significant Difference ◽  
Treatment Data ◽  
The Mean

Malacca plant (Phyllanthus emblica) is one of the medicinal plants. The purpose of this study was to determine the effect of n-hexane extract of Malacca (Phyllanthus emblica) leaves on the growth of Staphylococcus epidermidis bacteria in vivo. All mice were first induced by Staphylococcus epidermidis bacteria. Negative control (K1) was given aquadest, positive control (K2) was given ciproflaxacin suspension at doses of 20 mg/kg BW, while K3, K4, and K5 were given n-hexane extract of Malacca leave at dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Respectively blood sampling was carried out on the 5th day after treatment. Data were analyzed using one-way analysis of variance (ANOVA). The results showed that the mean (± SD) number of bacterial colonies in K1 was 656x10² cfu/ml. The average number of bacterial colonies in K2 was 2328x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 100 mg/kg BW on K3 was 359,60x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 200 mg/kg BW at K4 was 200x10² cfu/ml and the average number of bacterial colonies given n-hexane extract of malacca leave 300 mg/kg BW at K5 was 3483x10² cfu/ml. The results showed there were no significant difference among treatment groups (P 0.05). N-hexane extract of malacca leave was unable to inhibit the growth of Staphylococcus epidermidis bacteria in vivo

scholarly journals Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2

2021 ◽  
Vol 4 (2) ◽  
pp. 73
Author(s):  
Putri Ayu Ika Setiyowati ◽  
Rofiatun Solekha ◽  
Sri Bintang Sahara Mahaputra Kusuma Negara ◽  
Reny Rosalina
Keyword(s):  
Body Weight ◽  
Optimal Dose ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Optimal Dosage ◽  
Skin Injury ◽  
Treatment Groups

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used.  Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of  0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups  were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw.  In day 21 all group were injected with 0,2 ml of  pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.

The enhancement of Arg1 and activated ERβ expression in microglia HMC3 by induction of 96% ethanol extract of Marsilea crenata Presl. leaves

2020 ◽  
Vol 30 (6) ◽  
Author(s):  
Burhan Ma’arif ◽  
Mangestuti Agil ◽  
Hening Laswati
Keyword(s):  
Laser Scanning ◽  
Negative Relationship ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Scanning Microscope ◽  
Treatment Groups ◽  
Arginase 1 ◽  
Significant Difference ◽  
Ifn Γ

AbstractBackgroundPhytoestrogens have a high potential to overcome the neuroinflammation caused by estrogen deficiency. Marsilea crenata Presl. is a plant known to contain phytoestrogens. This research aimed to report the activity of a 96% ethanol extract of M. crenata leaves in inducing activation of microglia HMC3 cell to M2 polarity, which has anti-inflammatory characteristics.MethodsThe study was done by culturing microglia HMC3 cell in 24-well microplate and inducing it with IFN-γ for 24 h to activate the cell to M1 polarity, which has proinflammatory characteristics. The 96% ethanol extract was added with various doses of 62.5, 125, and 250 ppm. Genistein, 50 μM, was used as a positive control. The analysis of the immunofluorescence of Arginase-1 (Arg1) and ERβ as markers was done using a convocal laser scanning microscope.ResultsThe result of Arg1 shows a significant difference in Arg1 expression in the microglia HMC3 cell line between the negative control and all treatment groups at p < 0.05, with the best result at 250 ppm, whereas for ERβ, the results show, at doses of 125 and 250 ppm, that the 96% ethanol extract of M. crenata leaves decrease the activated ERβ expression at p < 0.05, with the best result at 250 ppm. The Arg1 and activated ERβ expression have a weak negative relationship with the Pearson correlation test.ConclusionsThe 96% ethanol extract of M. crenata leaves has an antineuroinflammation activity through the induction of Arg1 and activated ERβ expression in microglia HMC3 cell, with the best dose at 250 ppm.

scholarly journals The effect of noni fruit (Morinda citrifolia L.) extract on interleukin 4 and interleukin 10 in Wistar rats, induced by pyrazinamide, ethambutol and levofloxacin

2019 ◽  
Author(s):  
Thontowi Djauhari Nur Subchi ◽  
Merryana Andriani
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Cell Damage ◽  
Interleukin 10 ◽  
Positive Control ◽  
Negative Control ◽  
Morinda Citrifolia ◽  
Interleukin 4 ◽  
Test Results ◽  
Fruit Extract

Pyrazinamide, Ethambutol and Levofloxacin are part of the anti-tuberculosis SLD that have many side effects. This response causes an inflammatory reaction that affects pro-inflammatory cytokines interleukin 4 and interleukin 10. Morinda citrifolia L. produces anti-inflammatory activity that affects cytokines and provides protection against cell damage. A dose therapy was administered to wistar rats for two months, grouped in K- (negative control), K+ (positive control), MI (drug + Morinda citrifolia L. extract, dose of 100 mg/kg body weight), MII (drug + noni fruit extract, dose of 200 mg/kg body weight) and MIII (drug + noni fruit extract, dose of 400 mg/kg body weight). Results were examined using Rat IL-4 and IL-10 immunoassay Quantikine ELISA kits. The ANOVA (Analysis of Variance) test results showed that there was an overall IL-4 difference (sig<0.05); for IL-10 there was no great difference (sig>0.05); it was decrease trend was shown in MI and MII. However, by giving Morinda citrifolia L. extracts can significantly influence the IL-4 and IL-10, with a decreasing trend in MI and MII.

scholarly journals Anti-plasmodial Effect of C. limon and C. paradisi extracts on Plasmodium berghei-infected mice

2021 ◽  
Vol 8 (2) ◽  
pp. 026-033
Author(s):  
Michael Okpara Elom ◽  
Anthony Gideon Uche ◽  
Boniface Nwofoke Ukwah ◽  
Victor Udoh Usanga ◽  
Anthonia Ifeoma Okpara-Elom ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Combined Therapy ◽  
Parasite Clearance ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Citrus Limon ◽  
Citrus Paradisi ◽  
Treatment Groups ◽  
Significant Difference

Antiplasmodial effect of Citrus limon and Citrus paradisi extracts on Plasmodium berghei-infected mice was studied. Twenty five albino mice were randomized into five categories of G, L, GL, ACT (positive control) and NC which stand for grape, lemon, grape and lemon combined extracts, artemisinin combined therapy and negative control respectively. The NC group did not receive any intervention. Other treatments were administered orally for 12 days whereas administration of ACT lasted for 3 days. Blood was collected from the tail vein of the mice at a three day interval through venipuncture. Thick blood films were prepared and parasite densities were estimated using standard parasitological techniques. Results were analysed with ANOVA and Duncan multiple range tests. There was no significant difference (p>0.05) between parasite densities of the treatment groups and the negative control at baseline levels. However, as the treatment progressed from day 3 through day 9, there were significant reductions (p<0.05) in parasite densities among treatment groups when compared to the negative control. In this study, extracts of C. limon and C. paradisi in both single and combined strengths have been found to have antiplasmodial properties in mice. ACT possessed the highest antiplasmodial effect while C. limon as a single treatment ranked second in possession of antiplasmodial activity but exhibited increased RBC lysis. In combination, C. limoni and C. paradise extracts showed antiplasmodial activity that is slightly less than that exhibited by the lemon extract alone, but maintained normal RBC morphology whereas C. paradisi extract alone exhibited the lowest level of parasite clearance with atrophied red blood cells. Investigation of the effects of the extracts on liver, kidney and gastrointestinal tissues of mice is recommended before they could be prescribed as antimalaria for other animals and humans.

scholarly journals The effect of meniran herbs extract (Phyllantus niruri) to triglycerides blood level in wistar rats

2012 ◽  
Vol 10 (1) ◽  
pp. 23-27
Author(s):  
JUDO YUSTANTO KAHONO ◽  
KISRINI KISRINI ◽  
YUL MARIYAH
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Negative Control ◽  
Control Group ◽  
Control Group Design ◽  
Total Period ◽  
Group I ◽  
Significant Difference ◽  
Post Test ◽  
Group Ii

Kahono JY, Kisrini, Mariyah Y. 2012. The effect of meniran herbs (Phyllantus niruri) extract to blood triglyceride level in rats. Biofarmasi 10: 23-27. The aim of this experiment was to determine the effect of meniran herbs (Phyllantus niruri L.) extract to blood triglycerides level in Wistar rats (Rattus norvegicus). This experiment was a laboratory experiment with pre and post-test with a control group design. The samples were 30 male Wistar rats with ±200 gram of body weight and ±2 months old. The samples were divided into five groups by using a purposive random sampling technique and each group consisted of six rats. Group I as a negative control, group II as a positive control, and groups III, IV and V as meniran herbs extract groups with first dose, second dose, and third dose. All rats were simultaneously given with hyperlipemic feed. Group II was simultaneously administered with Cholvastin 0.5 mg/200 grams body weight/day. Groups III, IV and V were simultaneously treated with meniran herbs extract at dose of 25 mg, 50 mg and 100 mg/200 grams body weight/day. The total period of this experiment was 21 days. At the day 1st and 21st, blood was collected from rat orbital venous plexus. Serum was analyzed for their pre and post-test blood triglycerides. Statistical analysis was performed by one-way analysis of variance (ANOVA) followed by a post-hoc test. The value of p=0.002 (p<0.05) showed there was a significant difference between pre-test and post-test in delta means of blood triglycerides level among groups. Meniran herbs extract had a significant triglycerides lowering effect against blood triglycerides level of Wistar rats. The most potential of meniran herbs extract dose was 100 mg/200 gram body weight/day.

UJI AKTIVITAS ANALGETIK EKSTRAK ETANOL HERBA SEMBUKAN (Paederia foetida L.) PADA MENCIT PUTIH JANTAN (Mus musculus) YANG DIINDUKSI DENGAN ASAM ASETAT

2020 ◽  
Vol 5 (2) ◽  
pp. 358-363
Author(s):  
Triswanto Sentat ◽  
◽  
Fitri Handayani ◽  
Ellen Indraswari
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Paederia Foetida

Sembukan plants (Paederia foetida L.) are wild vines that are usually used by people as potential medicinal plants for pain. The purpose of this study was to determine the ethanol extract of sembukan’s herbal analgesic activity in male white mice (Mus musculus) and determine the optimal dose of the ethanol extract from sembukan’s herbal (Paederia foetida L.) which has the potential as an analgesic. Sembukan’s herbal is extracted with 70% ethanol solvent and an analgesic activity test is divided into 5 treatment groups namely positive control group (potassium diclofenac), negative control, dose I (80 mg / kg body weight), dose II (160 mg / kg body weight) ) and dose III (320 mg / kg body weight) by oral administration. Thirty minutes after administration, the mice were given an indicator of pain, 0.5% acetic acid. Analgesic power is calculated by counting the amount of stretching of mice for 1 hour. From the results of the study, the ethanol extract of sembukan’s herbal has analgesic activity in male white mice with percent dose analgesic power 35.18%, dose II 53.58% and dose III 68.98%. The optimal dose that has potential as an analgesic in male white mice is dose III with 68.98% analgesic power.

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