scholarly journals Analisis Pendugaan Bakteri Escherichia Coli pada Kerang Hijau (Perna Viridis) di Morosari, Demak Analysis of Estimated Abundance of Escherichia coli Bacteria in Green Mussels (Perna viridis) in Morosari, Demak

2020 ◽  
Vol 9 (1) ◽  
pp. 40-46
Author(s):  
Muhammad Rajes Katon ◽  
Anhar Solichin ◽  
Oktavianto Eko Jati
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Quality Standard ◽  
The Body ◽  
Coliform Bacteria ◽  
Perna Viridis ◽  
Filter Feeder ◽  
E Coli ◽  
Escherichia Coli Bacteria ◽  
Test Result

ABSTRAK Kerang hijau merupakan jenis hewan bertubuh lunak yang hidup menetap (sessile) dan mendapatkan makanan dengan menyaring (filter feeder). Hal ini mengakibatkan banyak mikroorganisme yang terakumulasi ke dalam tubuh kerang hijau, salah satunya yaitu kelompok bakteri Coliform. Bakteri Coliform dibagi menjadi dua yaitu fecal Coliform dan non fecal Coliform. Fecal Coliform berasal dari limbah kotoran hewan dan manusia contoh bakteri E. coli. Keberadaan bakteri E. coli di luar tubuh digunakan sebagai indikator sanitasi makanan dan minuman apakah tercemar atau tidak. Kondisi perairan Morosari mengalami penurunan kualitas air, hal tersebut disebabkan oleh limbah domestik penduduk yang langsung dibuang ke bantaran sungai. Hal ini memungkinkan adanya cemaran mikroba yang dapat membahayakan kesehatan seperti bakteri. Menurut SNI 01 7388 2009 nilai MPN bakteri E. coli untuk Mollusca, Crustacea, dan Echinodermata segar adalah <3/g. Tujuan dari penelitian ini yaitu mengetahui kelimpahan  bakteri E. coli pada kerang hijau dan untuk membandingkan kelimpahan bakteri E. coli dengan baku mutu SNI. Penelitian ini dilaksanakan pada bulan Juli 2019. Pengambilan sampel terdiri 9 sampel kerang hijau (kecil <60 mm, sedang 61-70 mm dan besar >70 mm) dengan 3 ekor kerang pada masing masing ukuran. Metode MPN merupakan  metode digunakan untuk analisis bakteri Coliform dan E. coli. Hasil Uji MPN yang didapat yaitu > 1100 MPN/g pada semua sampel. Kesimpulannya berdasarkan hasil uji MPN diperoleh nilai MPN yaitu >1100 MPN/g, hasil tersebut menunjukkan bahwa kelimpahan bakteri E. coli pada kerang hijau melebihi baku mutu yang ditetapkan berdasarkan SNI 01-7388 2009. ABSTRACTGreen mussels are the type of mollusk that live permanently (sessile) and gets food by filtering (filter feeder). It causes many microorganisms accumulating into the body of green mussels, one of them is a group of Coliform bacteria. Coliform bacteria are divided into two, namely fecal and non-fecal Coliform.Coliform is derived from the waste of animal and human i.e. E. coli bacteria. The existence of E. coli bacteria outside the body is used as an indicator of sanitation for food and drink.The condition of Morosari waters has decreased, it’s caused by the domestic waste of resident which is discharge into the riverbank directly.This allows microbial contamination that can endanger health such as bacteria. According to SNI 01-7388-2009 MPN value from E. coli bacteria for Mollusks, Crustaceans, and Echinoderms is <3/g.. The purpose of this study was to determined the abundance of E. coli bacteria in green mussels and to compared the abundance of E. coli bacteria with the quality standards of SNI. The research was conducted in July 2019. The samples were consisted of 3 types size of green mussels,  small (<60 mm), medium (61-70 mm) and large (>70 mm) and 3 mussels selcted from the each size. MPN method was used for the analysis of Coliform and E. coli bacteria. MPN test result was > 1100 MPN/g on all samples.The result showed,that the abundance of E. coli bacteria in green mussels exceed the quality standard based on SNI 01-7388 2009.  

scholarly journals Identifikasi Bakteri Escherichia coli Susu Kedelai Murni di Pasar Jodoh Kota Batam

2021 ◽  
Vol 7 (1) ◽  
pp. 25-30
Author(s):  
Sri Hainil ◽  
Trie Yuni Elfasyari ◽  
Rofina Intan Sulistya
Keyword(s):  
Escherichia Coli ◽  
The Body ◽  
National Standard ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Soy Milk ◽  
Normal Flora ◽  
Healthy Human ◽  
Probable Number ◽  
Escherichia Coli Bacteria

Pure soy milk is a highly nutritious drink; in general, pure soy milk contains excellent content for the body in the form of protein, iron, phosphorus, carbohydrates, fat, provitamin A and B vitamins. Causing pure soy milk to contain or be contaminated with microorganisms. one of the microorganisms found in drinks or water is the Coliform bacteria. An example of Coliform bacteria is Escherichia coli. Escherichia coli is a bacterium that lives in the human intestine; these bacteria live as normal flora or can be called a collection of microorganisms, naturally present in normal and healthy human bodies. The research objective was to determine the presence or absence of Escherichia coli in pure soy milk sold in Jodoh Market, Batam City. This study uses the Most Probable Number (MPN) method. The results of the study on 10 samples of pure soy milk A, B, C, D, E, F, G, H, I, J and 2 samples of soy milk branded K, and L contained coliform bacteria, and there were 6 Escherichia coli bacteria in soy milk samples. Pure A, B, C, D, G, and on samples of soy milk branded K. The results of the examination of 12 samples of soy milk were found not to meet the requirements of the Indonesian National Standard (INS 01 – 3830 – 1995).

scholarly journals Fethiye Bölgesinde Açıkta Satılan Tüketime Hazır Bazı Gıdalarda Koliform Bakteri Sayısının Belirlenmesi

2022 ◽  
Vol 9 (sp) ◽  
pp. 2552-2559
Author(s):  
Mehtap Çiftçi ◽  
Nilgün Öncül
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Food Industry ◽  
Ice Cream ◽  
Raw Milk ◽  
Coliform Bacteria ◽  
E Coli ◽  
Fecal Coliform Bacteria ◽  
Hygienic Measures ◽  
Intestinal Pathogens

Since coliform bacteria are common both in the intestine and in nature (soil, plant, etc.), they are considered as a sanitation indicator in the food industry. It is known that the majority of bacteria defined as fecal coliform in the coliform group are Escherichia coli. The presence of E. coli or fecal coliform bacteria in any sample is an indication that the necessary hygienic measures are not taken during production, storage, and sale. That means the sample is directly or indirectly contaminated with faeces, and/or other intestinal pathogens may also exist. In this study, raw milk, freshly squeezed fruit juices, unpackaged ice cream, shaved ice, and ice-cold samples were purchased from the famous touristic destination Fethiye and analyzed for coliform bacteria. For this purpose, the samples were purchased from local marketplaces, buffets, cafes, patisseries, restaurants, and roadsides at Fethiye. In total 60 samples were analyzed using Violet Red Bile (VRB) Agar. The results of coliform bacteria ranged

scholarly journals Evaluation of the Effectiveness of the Activated Sludge Process in the Elimination Both ATB-Resistant and ATB-Susceptible E. coli Strains

Energies ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5868
Author(s):  
Iwona Beata Paśmionka ◽  
Karol Bulski ◽  
Piotr Herbut ◽  
Elżbieta Boligłowa ◽  
Frederico Márcio C. Vieira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Activated Sludge ◽  
Fecal Coliform ◽  
Antimicrobial Agents ◽  
Treated Wastewater ◽  
Coliform Bacteria ◽  
Diffusion Method ◽  
Activated Sludge Process ◽  
E Coli ◽  
Fecal Coliform Bacteria

Water reuse is now becoming a global necessity. However, one of the drawbacks in releasing wastewater into the environment is some persistent pollutants that are not completely removed in wastewater treatment plant. Residual bacteria and antibiotics in the inflowing wastewater can contribute to the antibiotic resistance spread in the aquatic environment. This study determined the effectiveness of activated sludge process for fecal coliform bacteria elimination, and also the Escherichia coli resistance to antimicrobial agents as erythromycin, azithromycin, clarithromycin, ofloxacin, ciprofloxacin, trimethoprim, and metronidazole in treated wastewater. The research was carried out using the membrane filtration technique, and the susceptibility of isolates to antimicrobial agents was tested by the disc diffusion method. The concentrations of fecal coliform bacteria and Escherichia coli differed significantly depending on the seasonal period in which it was carried out. Despite up to 99% reduction in the number of sanitary indicators in biologically treated wastewater, 89% of E. coli isolates resistant to the tested antibiotics was found, while 100% of the isolates were susceptible to metronidazole. Most of the isolates showed resistance to trimethoprim, and the fewest isolates were resistant to ofloxacin, indicating that some strains may react differently to antibiotics.

Bacteriophages reduce Escherichia coli O157:H7 levels in experimentally inoculated sheep

2009 ◽  
Vol 89 (2) ◽  
pp. 285-293 ◽  
Author(s):  
S J Bach ◽  
R P Johnson ◽  
K. Stanford ◽  
T A McAllister
Keyword(s):  
Escherichia Coli ◽  
Oral Administration ◽  
Coliform Bacteria ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Total Coliforms ◽  
E Coli ◽  
Oral Inoculation ◽  
Experimental Treatments ◽  
And Control

Bacteriophage biocontrol has potential as a means of mitigating the prevalence of Escherichia coli O157:H7 in ruminants. The efficacy of oral administration of bacteriophages for reducing fecal shedding of E. coli O157:H7 by sheep was evaluated using 20 Canadian Arcott rams (50.0 ± 3.0) housed in four rooms (n = 5) in a contained facility. The rams had ad libitum access to drinking water and a pelleted barley-based total mixed ration, delivered once daily. Experimental treatments consisted of administration of E. coli O157:H7 (O157), E. coli O157:H7+bacteriophages (O157+phage), bacteriophages (phage), and control (CON). Oral inoculation of the rams with 109 CFU of a mixture of four nalidixic acid-resistant strains of E. coli O157:H7 was performed on day 0. A mixture of 1010 PFU of bacteriophages P5, P8 and P11 was administered on days -2, -1, 0, 6 and 7. Fecal samples collected on 14 occasions over 21 d were analyzed for E. coli O157:H7, total E. coli, total coliforms and bacteriophages. Sheep in treatment O157+phage shed fewer (P < 0.05) E. coli O157:H7 than did sheep in treatment O157. Populations of total coliforms and total E. coli were similar (P < 0.05) among treatments, implying that bacteriophage lysis of non-target E. coli and coliform bacteria in the gastrointestinal tract did not occur. Bacteriophage numbers declined rapidly over 21 d, which likely reduced the chance of collision between bacteria and bacteriophage. Oral administration of bacteriophages reduced shedding of E. coli O157:H7 by sheep, but a delivery system that would protect bacteriophages during passage through the intestine may increase the effectiveness of this strategy as well as allow phage to be administered in the feed.Key words: Escherichia coli O157:H7, bacteriophage, sheep, environment, coliforms

scholarly journals Comparison of Fecal Coliform Agar and Violet Red Bile Lactose Agar for Fecal Coliform Enumeration in Foods

2002 ◽  
Vol 68 (4) ◽  
pp. 1631-1638 ◽  
Author(s):  
A. Leclercq ◽  
C. Wanegue ◽  
P. Baylac
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Food Samples ◽  
Fecal Coliforms ◽  
Predictive Values ◽  
Direct Plating ◽  
Hafnia Alvei ◽  
E Coli ◽  
Plating Method ◽  
Mashed Potatoes

ABSTRACT A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 ± 1°C and transfer to 44 ± 1°C for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as Hafnia alvei, which could form typical colonies on FCA and VRBL. Recovery of cold-stressed Escherichia coli in mashed potatoes on FCA was about 1 log unit lower than that with VRBL. When the FCA method was compared with standard VRBL for enumeration of fecal coliforms, based on counting carried out on 170 different food samples, results were not significantly different (P > 0.05). Based on 203 typical identified colonies selected as found on VRBL and FCA, the latter medium appears to allow the enumeration of more true fecal coliforms and has higher performance in certain ways (specificity, sensitivity, and negative and positive predictive values) than VRBL. Most colonies clearly identified on both media were E. coli and H. alvei, a non-fecal coliform. Therefore, the replacement of fecal coliform enumeration by E. coli enumeration to estimate food sanitary quality should be recommended.

Fate of Escherichia coli O157:H7 and Other Coliforms in Commercial Mayonnaise and Refrigerated Salad Dressing

1995 ◽  
Vol 58 (1) ◽  
pp. 13-18 ◽  
Author(s):  
ERROL V. RAGHUBEER ◽  
JIM S. KE ◽  
MICHAEL L. CAMPBELL ◽  
RICHARD S. MEYER
Keyword(s):  
Escherichia Coli ◽  
Storage Temperature ◽  
Enterobacter Aerogenes ◽  
Antimicrobial Effect ◽  
Coliform Bacteria ◽  
Escherichia Coli O157 ◽  
Salad Dressing ◽  
E Coli ◽  
Survival Pattern ◽  
High Level

Commercial mayonnaise and refrigerated ranch salad dressing were inoculated at two levels with two strains of Escherichia coli O157:H7, a non-pathogenic E. coli, and the non-fecal coliform Enterobacter aerogenes. Results showed that at the high inoculation level (&gt;106 colony forming units [CFU]/g) in mayonnaise stored at room temperature (ca. 22°C) both strains of O157:H7 were undetected at 96 h. At the high inoculation level, all strains of coliform bacteria tested survived longer in salad dressing stored at 4°C than in mayonnaise stored at 22°C. The O157:H7 strains were still present at low levels after 17 days. The survival time in the low-level inoculum (104CFU/g) study decreased, but the survival pattern in the two products was similar to that observed in the high-level inoculum study. Slight differences in survival among strains were observed. The greater antimicrobial effect of mayonnaise may be attributable to differences in pH, water activity (aw), nutrients, storage temperature, and the presence of lysozyme in the whole eggs used in the production of commercial mayonnaise. Coliform bacteria survived longer in refrigerated salad dressing than in mayonnaise particularly at the high-level inoculum. Both mayonnaise (pH 3.91) and salad dressing (pH 4.51) did not support the growth of any of the microorganisms even though survival was observed.

scholarly journals Evaluation of antipyretic activity of hydroalcoholic extract of Corchorus depressus Linn. in Escherichia coli–induced pyretic rabbits

2018 ◽  
Vol 16 ◽  
pp. 205873921879295
Author(s):  
Saeed Ahmad ◽  
Muhammad Akram ◽  
Syed Muhammad Ali Shah ◽  
Sabira Sultana
Keyword(s):  
Escherichia Coli ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Hydroalcoholic Extract ◽  
Antipyretic Effect ◽  
E Coli ◽  
Antipyretic Activity ◽  
Experimental Treatments

This study was conducted to investigate the antipyretic effect of the hydroalcoholic extract of Corchorus depressus Linn. against Escherichia coli ( E. coli)-induced pyrexia in rabbits. Hydroalcohalic extracts of C. depressus were given orally at 25, 50, and 100 mg/kg for antipyretic affect in E. coli-induced fever in rabbits. The animals were divided into five groups of five each. Among these five groups, three received various doses of experimental treatments, whereas the fourth one served as positive control and received paracetamol. The fifth group of animals served as negative control and received no treatment. The body temperature of the rabbits was measured rectally over a period of 5 h. C. depressus exhibited better effects at dose rate of 25, 50, and 100 mg/kg. The hydroalcoholic extract of C. depressus has significant antipyretic effect. These results lend support to the popular use of C. depressus in traditional medicine as a remedy for pyrexia and suggest that the characterization of the principles for such activity deserves further investigation.

scholarly journals GREEN SYNTHESIS OF MAGNETIC IRON NANOPARTICLES USING MEDICINAL PLANT TRIDAX PROCUMBENS LEAF EXTRACTS AND ITS APPLICATION AS AN ANTIMICROBIAL AGENT AGAINST E. COLI

2020 ◽  
pp. 34-39
Author(s):  
YOJANA Y. PATIL ◽  
VAISHNVI B. SUTAR ◽  
ARPITA P. TIWARI
Keyword(s):  
Escherichia Coli ◽  
Magnetic Nanoparticles ◽  
Iron Nanoparticles ◽  
Most Probable Number ◽  
Leaf Extracts ◽  
Probable Number ◽  
Gram Negative ◽  
E Coli ◽  
Tridax Procumbens ◽  
Escherichia Coli Bacteria

Objective: The present study was aimed at the biological synthesis of magnetic iron nanoparticles by using the plant extract of Tridax procumbens and also to study their antimicrobial property against gram-negative bacteria (Escherichia coli). Methods: The synthesis of magnetic iron nanoparticles was carried out by the co-precipitation method using biological methods like plant extract as reducing agent and capping agents are biocompatible and non-hazardous. These nanoparticles were characterized by UV-Visible spectroscopy, XRD (X-Ray Diffraction), and SEM (Scanning Electron Microscope). As well as antibacterial activity of the nanoparticles was carried out by agar well diffusion method and Most Probable Number (MPN) method against gram-negative E. coli (Escherichia coli) bacteria. Results: The average crystallite size of Magnetic Nanoparticles (MNPs) was found to be 72 nm by X-ray diffraction. The optical absorption band at wavelengths of 240 nm and 402 nm was obtained from the UV Visible spectrum. Spherical shape morphology was observed in SEM studies. The antibacterial assay clearly expressed that E. coli showed a maximum zone of inhibition (15±0.15 mm) at 2 mg/ml and 1 mg/ml concentration was found for Magnetic Nanoparticles. In the Most Probable Number (MPN) test it is seen that the bacterial count is reduced after adding synthesized NPs into the water sample. Conclusion: The results of the present study conclude that the Magnetic Nanoparticles synthesized using Tridax procumbens leaf extracts is found to be stable and show good antibacterial activity against gram-negative (Escherichia coli) bacteria.

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

scholarly journals VERIFIKASI PENENTUAN ANGKA BAKTERI Escherichia coli PADA SAMPEL YANG DI-SPIKE MENGGUNAKAN METODE COLONY FORMING UNIT

2019 ◽  
Vol 6 (1) ◽  
pp. 42-48
Author(s):  
Chairani Arizal ◽  
Aswal Harianto
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Coliform Bacterium ◽  
High Dilution ◽  
Bacterial Growth Rate ◽  
Accuracy Test ◽  
Escherichia Coli Bacteria ◽  
Limit Sensitivity ◽  
Use Factors

The water that must be drunk is healthy water that meets the requirements of Bacteriology, Chemistry, Radioactivity and Physicality based on Republic of Indonesia Minister of Health Regulation No: 492 / MENKES / PER / IV / 2010 concerning the requirements and supervision of clean water quality which includes physical requirements which are odorless, not colored and tasteless, where for the Coliform value is 0/100 mL. Escherichia coli is a fecal coliform bacterium and an indicator of the quality of drinking water because its presence in water indicates that the water is contaminated by feces. The aim of this study was to determine the value of accuracy, precision, detection limit, sensitivity and specificity of the calculated numbers of Escherichia coli bacteria from spike samples using the CFU method with agar chromocult media. The results of the bacterial results obtained on accuracy parameters were 229.33%, precision ie 26.35%, LOD which was 13.85 CFU / ml, LOQ ie 138.54 CFU / ml, and Specificity of 0%. Based on the results of verification of bacterial numbers obtained from the results of the accuracy test, precision that is not within the range of  general requirements indicates that the method cannot be valid to use factors that affect high dilution, rapid bacterial growth rate, adequate nutrition and observer vision, whereas specificity indicate that chromocult coliform is very specific to Escherichia coli.

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