scholarly journals Chemical composition of essential oil in Mosla chinensis Maxim cv. Jiangxiangru and its inhibitory effect on Staphylococcus aureus biofilm formation

2018 ◽  
Vol 13 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Liang Peng ◽  
Yunhao Xiong ◽  
Mei Wang ◽  
Manman Han ◽  
Weilan Cai ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Chemical Composition ◽  
Essential Oil ◽  
Biofilm Formation ◽  
Quantitative Method ◽  
Inhibitory Concentration ◽  
Inhibitory Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Major Chemical

AbstractThe essential oil of Mosla chinensis Maxim cv. Jiangxiangru is known for its antibacterial ability. This study aimed to investigate the chemical composition of Jiangxiangru essential oil and its inhibitory effect on Staphylococcus aureus biofilm formation. Gas chromatography/mass spectrometry (GC–MS) was used to determine the chemical composition of Jiangxiangru essential oil. Subsequently, the eight major chemical components were quantitatively analyzed using GC– MS, and their minimum inhibitory concentration (MIC) values against S. aureus were tested. Biofilm formation was detected by crystal violet semi-quantitative method and silver staining. Of the 59 peaks detected, 29 were identified by GC–MS. Of these peaks, thymol, carvacrol, p-cymene, γ-terpinene, thymol acetate, α-caryophyllene, 3-carene, and carvacryl acetate were present at a relatively higher concentration. The results of the quantitative test showed that thymol, carvacrol, p-cymene, and γ-terpinene were the major components of the essential oil. Among the eight reference substances, only thymol, carvacrol, and thymol acetate had lower MICs compared with the essential oil. Essential oil, carvacrol, carvacryl acetate, α-caryophyllene, and 3-carene showed the better inhibition of S. aureus biofilm formation. When one fourth of the MIC concentrations were used for these substances (0.0625 mg/mL for essential oil, 0.0305 mg/mL for carvacrol, 1.458 mg/mL for carvacryl acetate, 0.1268 mg/mL for α-caryophyllene, and 2.5975 mg/mL for 3-carene), the inhibition rates were over 80%. However, thymol, γ-terpinene, thymol acetate, and p-cymene showed a relatively poor inhibition of S. aureus biofilm formation. When 1× MIC concentrations of these substances were used, the inhibition rates were less than 50%. In conclusion, Jiangxiangru essential oil and its major components, carvacrol, carvacryl acetate, α-caryophyllene, and 3-carene, strongly inhibited biofilm formation in S. aureus.

Rosemary and Tea Tree Essential Oils Exert Antibiofilm Activities In Vitro against Staphylococcus aureus and Escherichia coli

2020 ◽  
Vol 83 (7) ◽  
pp. 1261-1267
Author(s):  
TING LIU ◽  
JINGFAN WANG ◽  
XIAOMAN GONG ◽  
XIAOXIA WU ◽  
LIU LIU ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Biofilm Formation ◽  
Gas Chromatography Mass Spectrometry ◽  
Tea Tree ◽  
E Coli ◽  
Microdilution Method ◽  
Broth Microdilution Method

ABSTRACT The purpose of the present study was to determine the bioactive compounds in rosemary essential oil (REO) and tea tree essential oil (TEO) and to investigate their antibacterial and antibiofilm activities against Staphylococcus aureus and Escherichia coli in vitro. The MIC and MBC assays were performed to assess the antibacterial activity of these two EOs against S. aureus and E. coli with the broth microdilution method. A crystal violet assay was used to ascertain the effects of EOs on the biofilm formation of the test strains, and a tetrazolium bromide (MTT) assay was used to measure the level of inactivation of mature biofilms by EOs. Gas chromatography–mass spectrometry revealed 15 compounds in REO and 27 compounds in TEO, representing 97.78 and 98.13% of the total EO, respectively. Eucalyptol and α-pinene were found in high concentrations in REO, and the two major compounds in TEO were 4-terpineol and terpinolene. The MICs of REO for the two S. aureus and E. coli test strains were both 0.5 mg/mL, and the MICs of TEO for the two strains were both 0.25 mg/mL. Therefore, these EOs can significantly inhibit the formation of biofilms and induced morphological biofilm changes, as verified by scanning electron microscopy. Both EOs had destructive effects on the mature biofilm of the two test strains. TEO was more inhibitory than REO for biofilm formation by the two test strains. HIGHLIGHTS

scholarly journals Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms

2007 ◽  
Vol 56 (4) ◽  
pp. 519-523 ◽  
Author(s):  
Antonia Nostro ◽  
Andrea Sudano Roccaro ◽  
Giuseppe Bisignano ◽  
Andreana Marino ◽  
Maria A. Cannatelli ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Oregano Essential Oil ◽  
Planktonic Growth ◽  
Subinhibitory Concentrations

The aim of this study was to evaluate the effect of oregano essential oil, carvacrol and thymol on biofilm-grown Staphylococcus aureus and Staphylococcus epidermidis strains, as well as the effects of the oils on biofilm formation. For most of the S. aureus (n=6) and S. epidermidis (n=6) strains tested, the biofilm inhibitory concentration (0.125–0.500 %, v/v, for oregano, and 0.031–0.125 %, v/v, for carvacrol and thymol) and biofilm eradication concentration (0.25–1.0 %, v/v, for oregano and 0.125–0.500 %, v/v, for carvacrol and thymol) values were twofold or fourfold greater than the concentration required to inhibit planktonic growth. Subinhibitory concentrations of the oils attenuated biofilm formation of S. aureus and S. epidermidis strains on polystyrene microtitre plates.

scholarly journals Behavioural activities and chemical composition of fresh leaf essential oil of Plectranthus aegyptiacus from Southwest Nigeria in mice

2021 ◽  
Vol 14 (2) ◽  
pp. 064-076
Author(s):  
Enimeya Dressman Akuegbe ◽  
Idris Ajayi Oyemitan ◽  
Ifeoluwa Isaac Ogunlowo ◽  
Gugulethu Mathew Miya ◽  
Opeoluwa Oyehan Oyedeji ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Inhibitory Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Gabaergic Neurotransmission ◽  
Fresh Leaf ◽  
Central Nervous System Depressant ◽  
Southwest Nigeria ◽  
Leaf Essential Oil ◽  
Hole Board

Objectives: This study determined the chemical composition of essential oil obtained from fresh leaf of Plectranthus aegyptiacus, and evaluated it for novelty-induced behavioural (NIB) and determine its mechanism(s) of action in mice. Methods: The oil was hydro-distillated, and analyzed using gas chromatography-mass spectrometry. The effects of the oil (50, 100 and 150 mg/kg; i.p., n=6) on novelty-induced behavioural was assessed using open field test and head dipping on hole board. Probable mechanism(s) were evaluated using antagonists: flumazenil, naloxone and cyproheptadine at 2 mg/kg each, atropine and yohimbine at 5 mg/kg and 1 mg/kg respectively. Key Findings: The LD50 values obtained were 2154 and 490 mg/kg for oral and intraperitoneal routes respectively. The oil (50, 100 and150 mg/kg) significantly (p<0.05, 0.01 and 0.01) inhibited all NIB and head dips. Flumazenil significantly (p<0.05) reversed the effect of the oil on NIB; atropine, naloxone and cyproheptadine significantly (p<0.01, 0.01 and 0.001) potentiated inhibitory effect on NIB respectively, while yohimbine showed no significantly effect. The analyzed oil showed 61 compounds, and the major compounds were carvacrol, germacrene-D, p-cymene and [1,1'-Bicyclopentyl]-2,2'-diol. Conclusions: The study concluded that the oil possessed central nervous system depressant activity, which could be mediated mainly through augmentation of GABAergic neurotransmission, while cholinergic-(muscarinic), adrenergic and serotonergic pathways may be involved.

scholarly journals Comparison of Chemical Composition between Kuromoji (Lindera umbellata) Essential Oil and Hydrosol and Determination of the Deodorizing Effect

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4195
Author(s):  
Naoki Nanashima ◽  
Maiko Kitajima ◽  
Shizuka Takamagi ◽  
Miyuki Fujioka ◽  
Toshiko Tomisawa
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Isovaleric Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Methyl Mercaptan ◽  
Foot Care

Kuromoji (Lindera umbellata) is a tree that grows throughout Japan. The components of kuromoji essential oil have antitumor and aromatherapy effects. However, the composition of the hydrosol, obtained as a by-product of the essential oil process, is unknown. Furthermore, it is unknown whether kuromoji essential oil has a deodorizing effect. Therefore, the purpose of the current study was to compare the chemical composition of kuromoji essential oil and hydrosol, as well as evaluate the deodorizing effect of the former. The chemical composition of samples was evaluated using gas chromatography–mass spectrometry (GC-MS). Additionally, the deodorizing effect of Kuromoji essential oil was investigated with the detector tube method using ammonia, hydrogen sulfide, methyl mercaptan, and isovaleric acid. Linalool was the most abundant component in both the essential oil and hydrosol; however, its proportion was higher in the hydrosol (57.5%) than in the essential oil (42.8%). The hydrosol contained fewer chemical components, but higher proportions of trans-geraniol and ethanol. Moreover, the essential oil eliminated 50% of ammonia and 97.6% or more of isovaleric acid. Interestingly, linalool was soluble in the hydrosol and did not irritate the skin. This suggests that the hydrosol may be an effective foot care product.

scholarly journals Formulation and Evaluation of Nagarmotha Hair Oil

2021 ◽  
Vol 9 (12) ◽  
pp. 1107-1113
Author(s):  
Miss. Patil Shivani Navnath
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Pharmacological Activity ◽  
Cyperus Rotundus ◽  
Chemical Components ◽  
Therapeutic Action ◽  
Extensive Review ◽  
The Family ◽  
Anti Inflammatory ◽  
Major Chemical

Abstract: The Presenace study investigation oil isolation form cyperus rotundus and the chemical composition of essential oil of root's of Cyprus rotundus.In india it's commonly known as nagarmotha.and it belongs to the family cyperacea.the major chemical components of this herb are essential oils.flavonoids, terpenoids, sesauiterpenes, cyproten, cyperene, aselinene, rotundene, valencene, cyperol, gurjunene, transcalamenenene, cad'Alene, cyperoutundone, mustskone, isocyperol, acyperone etc. Reserch studies have shown that it possesses various Pharmacological activity Such as diuretic,carmenative emmenagogue, anthelmintic, analgesic, anti-inflammatory, anti-dysentery, anti-rheumatic activities.an extensive review of the ancient traditional literature and madern research revealead that the drug has numerous therapeutic action. Several of which have been established scientifically, which may help the reserchers to set their minds for approaching the utility efficiency and potency of nagarmotha. Keywords: cyperus rotundus, cyprotene flavanoids nagarmotha.

scholarly journals Chemical Composition and Vasorelaxant and Antispasmodic Effects of Essential Oil fromRosa indicaL. Petals

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Hafiz Majid Rasheed ◽  
Taous Khan ◽  
Fazli Wahid ◽  
Rasool Khan ◽  
Abdul Jabbar Shah
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Acid Ethyl Ester ◽  
Rabbit Aorta ◽  
Cardiovascular Effects ◽  
Scientific Data ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenyl Acetate ◽  
Chemical Components ◽  
Electrophysiological Measurements

Rosa indicaL. belongs to the family Rosaceae and is locally known as gulaab. It has different traditional uses in cardiovascular and gastrointestinal disorders but there is no scientific data available in this regard. Therefore, the basic aim of this study was to explore the chemical composition and gastrointestinal and cardiovascular effects of the essential oil obtained fromR. indica. The chemical composition of the essential oil was investigated using gas chromatography-mass spectrometry (GC-MS) technique. The cardiovascular and gastrointestinal effects were investigated using electrophysiological measurements. The GC-MS analysis of the essential oil showed various chemical components including acetic acid, mercaptohexyl ester, butanoic acid, 2-methyl-5-oxo-1-cyclopentene-1-yl ester, artemiseole, methyl santonilate, isosteviol, caryophyllene oxide, pentyl phenyl acetate, dihydromyrcene, 1,5-octadecadien, octadecanoic acid, ethyl ester, palmitic acid (2-phenyl-1,3-dioxolan-4-yl methyl ester), santolina epoxide, and 9-farnesene. The electrophysiological measurements revealed that essential oil was more potent against K+(80 mM) than phenylephrine precontractions using isolated rabbit aorta preparations. In isolated rabbit jejunum preparations, it showed more potency against high K+induced contractions than spontaneous contractions. Considering these evidences, it can be concluded thatR. indicaessential oil may work as a complementary and alternative medicine in gastrointestinal and cardiovascular diseases.

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil from Qiancao (Rubia cordifolia Linn.) Roots against Selected Foodborne Pathogens

2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Wei-Qin Li ◽  
Mei-Ping Quan ◽  
Qin Li
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Foodborne Pathogens ◽  
Microbial Growth ◽  
Inhibitory Concentration ◽  
Gas Chromatography Mass Spectrometry ◽  
Natural Antimicrobial ◽  
Food Borne Pathogens ◽  
Food Borne

Chemical composition of the essential oil from Qiancao roots was analyzed by gas chromatography-mass spectrometry (GC-MS), and 43 components were identified in the paper. Mollugin (30.58%) was found to be the major component, followed by 3-methyl-2-cyclopenten-1-one (10.55%), 4-tert-butyl-2-phenyl-phenol (8.64%) and eugenol (6.30%). The antibacterial activity of essential oil against selected food-borne pathogens was evaluated. The results showed that the sensitivity to essential oil were different for different tested bacteria and the essential oil had a strong antibacterial activity against B. cereus with the lowest minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) values of 0.0625 and 0.125 mg/mL, respectively. In addition, microbial growth curve assay also showed that the essential oil had a significant effect on the growth rate of surviving B. cereus. The data of this study suggests that the essential oil from Qiancao roots has great potential for application as a natural antimicrobial agent to preserve food.

Chemical Composition and Antimicrobial Activity of the Saharo-endemic Species: Ammodaucus leucotrichus subsp. leucotrichus Coss & Dur (Apiaceae)

2018 ◽  
Vol 16 (S1) ◽  
pp. S104-S108 ◽  
Author(s):  
M. Touaibia ◽  
F. Saidi ◽  
D. Abdellali ◽  
F.Z. Raber Elmaizi
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Candida Krusei ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis

Essential oil of Ammodaucus leucotrichus subsp. leucotrichus Coss & Dur (endemic), extracted from the seeds by hydrodistillation, was screened for its possible antimicrobial activity as well as its chemical composition. According to the Gas Chromatography/Mass Spectrometry analysis, 35 components were identified (98.23%), perillaldehyde (45.58%) and limonene (14.02%) being the major components. The antimicrobial test of the essential oil showed that Staphylococcus aureus (MIC = 4.5 mg/ml) and Bacillus cereus (MIC = 9 mg/ml) were the most sensitive strains, followed by Candida krusei and Candida albicans (MIC = 18 mg/ml both).

scholarly journals Chemical Composition and Biological Activity of Essential Oil from Pulicaria undulata from Yemen

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Nasser A. Awadh Ali ◽  
Farukh S. Sharopov ◽  
Mehdi Alhaj ◽  
Gabrielle M. Hill ◽  
Andrea Porzel ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Inhibitory Effect ◽  
Disc Diffusion ◽  
Methicillin Resistant ◽  
Breast Tumor Cells ◽  
Cladosporium Cucumerinum ◽  
Mcf 7

The chemical composition of the essential oil obtained from the leaves of Pulicaria undulata Gamal Ed Din (syn P. oriental sensu Schwartz and P. jaubertii Gamal Ed Din) was analyzed by GC-MS. Major compounds of P. undulata oil were the oxygenated monoterpenenes, carvotanacetone (91.4%) and 2,5-dimethoxy-p-cymene (2.6.%). The antimicrobial activity of the essential oil was evaluated against six microorganisms, Escherichia coli Pseudomonas aeruginosa, Staphylococcus aureus, methicillin-resistant S. aureus, Bacillus subtilis, and Candida albicans, using disc diffusion and broth microdilution methods. The oil showed the strongest bactericidal activity against Staphylococcus aureus and methicillin-resistant S. aureus, as well as Candida albicans. The essential oil showed moderate cytotoxic activity against MCF-7 breast tumor cells, with an IC50 of 64.6 ±13.7 μg/mL. Bioautographic assays were used to evaluate the acetylcholinesterase inhibitory effect as well as antifungal activity of the oil against Cladosporium cucumerinum.

scholarly journals Cantinoa althaeifolia essential oil: chemical composition and biological, antioxidant, antimicrobial, and antitumor activities

2021 ◽  
Vol 10 (2) ◽  
pp. e9910212040
Author(s):  
Amanda Migliorini Urban ◽  
Juliane Nadal Dias Swiech ◽  
Gustavo Simão Moraes ◽  
Katia Paludo ◽  
Carmen Antonia Sanches Ito ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Glandular Trichomes ◽  
Secondary Compounds ◽  
Artemia Salina ◽  
Gas Chromatography Mass Spectrometry ◽  
Complex Method ◽  
Antitumor Activities

The species of the Lamiaceae family produce essential oils in the glandular trichomes, leaf surfaces, and in inflorescences, and can also produce many secondary compounds, which indicates great potential for study. The aim of this paper was to describe the chemical composition and the biological, antioxidant, antimicrobial, and antitumor activities of the essential oil of Cantinoa althaeifolia for the first time. The essential oil was obtained from the leaves by hydrodistillation and analyzed by gas chromatography mass spectrometry (GC/MS). The antioxidant activity was established by the phosphomolybdenum method. The minimum inhibitory concentration against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans was determined. The lethality test of Artemia salina was performed. The cytotoxicity against murine fibroblasts (3T3), non-tumor cells (McCoy), murine melanoma (B16F10), and human breast carcinoma (MCF-7) cells was assessed. Five major compounds were isolated: himachalene (11.62%), spathulenol (10.08%), caryophylline oxide (9.93%), (E)-caryophylene (9.21%), and pogostol (6.31%). The phosphomolybdenum complex method showed positive results. The minimum inhibitory concentration was greater than 1000 μg/mL for the tested microorganisms. The mortality rate of Artemia salina was low. The tested cancer cell lines showed an IC50 of 12.5 and 25 μg/mL. These findings indicate that the essential oil of Cantinoa althaeifolia has antioxidant, antimicrobial, and antitumor activities.

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