Concentrations of homocysteine, related metabolites and asymmetric dimethylarginine in preeclamptic women with poor nutritional status

2005 ◽  
Vol 43 (10) ◽  
Author(s):  
Wolfgang Herrmann ◽  
Sonia Isber ◽  
Rima Obeid ◽  
Markus Herrmann ◽  
Muhidien Jouma
Keyword(s):  
Nutritional Status ◽  
Asymmetric Dimethylarginine ◽  
Plasma Concentrations ◽  
Elevated Serum ◽  
B Vitamins ◽  
Serum Folate ◽  
Serum Concentrations ◽  
Folate Supplementation ◽  
Proxy Measure ◽  
Early Onset Preeclampsia

AbstractBackground: Hyperhomocysteinemia, a proxy measure for the nutritional status of the B vitamins, may be involved in the etiology of preeclampsia via inducing endothelial dysfunction. Asymmetric dimethylarginine (ADMA) is an inhibitor of NO-synthase that may adversely affect the endothelium.Materials and methods: We investigated serum concentrations of folate, vitamin BResults: Higher concentrations of Hcy, cystathionine and methylmalonic acid (MMA) were closely linked to a lower status of the B vitamins. Higher concentrations of Hcy and cystathionine were observed in the preeclamptic group than in the matched controls (median Hcy 9.3 vs. 6.0μmol/L; median cystathionine 284 vs. 232nmol/L). Serum folate was significantly lower in patients than in controls (16.4 vs. 36.0nmol/L). Folate supplementation was less likely to be used in preeclamptic women. Concentrations of MMA were elevated in patients and controls and did not differ significantly between the two groups. Median plasma concentrations of ADMA were significantly lower in asymptomatic women than in those who developed preeclampsia before the 37th week of gestation (0.61 vs. 0.68μmol/L).Conclusions: Elevated serum concentrations of Hcy, cystathionine and MMA indicate poor status of the B vitamins during pregnancy. The adverse effect of Hcy on endothelial function might be related to ADMA in early-onset preeclampsia. More emphasis should be placed on increasing the intake of B vitamins in pregnant women from developing countries.

Adequate intake and supplementation of B vitamins, in particular folic acid, can play a protective role in bone health

2021 ◽  
Vol 14 ◽  
Author(s):  
Mariangela Rondanelli ◽  
Alice Tartara ◽  
Federica Fossari ◽  
Viviana Vecchio ◽  
Milena Anna Faliva ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Bone Health ◽  
B Vitamins ◽  
Folate Intake ◽  
Serum Folate ◽  
Blood Levels ◽  
Negative Consequences ◽  
Mineral Density ◽  
Folate Supplementation

: In vitro and animal model studies have shown that B vitamins (VB) deficiency have negative consequences on bone, as a result of direct or mediated activity of hyperhomocysteinemia. However, there are still no precise indications regarding a possible VB role in order to maintain bone health. So, the aim of this narrative review was to consider the state of the art on correlation between VB dietary intake, blood levels and supplementation and bone health (bone mineral density (BMD), bone turnover markers and fractures risk) in humans. This review included 29 eligible studies. Considering VB blood levels, the 14 studies considered have shown that low serum folate can be a factor risk for reduced BMD and fractures in elderly, particularly women; no independent association was found for other VB. Studies that evaluate relationship between VB dietary intake and BMD are only 2; one, conducted on 1869 women, demonstrated a positive effect of folate intake on BMD, another demonstrated a dose-dependent inverse relationship between vitamin B6 dietary intake and risk of hip fracture, but only for 35298 female participants. Regarding the relationship between BV supplementation and bone health (9 studies with only VB and 4 with other nutrients), all studies that considered patients with hyperhomocysteinemia or with low folate blood levels, are in agreement in demonstrating that folate supplementation (500mcg-5mg) is useful in improving BMD. In conclusion, a request for folate and homocysteine blood levels in elderly patients with osteopenia/osteoporosis is mandatory. For patients with hyperhomocysteinemia or with low folate blood levels, folate supplementation (500mcg-5mg) is crucial.

scholarly journals Daily supplementation with 5 mg of folic acid in Brazilian patients with hereditary spherocytosis

2019 ◽  
Vol 67 (8) ◽  
pp. 1110-1117 ◽  
Author(s):  
Clóvis Paniz ◽  
Maylla Rodrigues Lucena ◽  
Juliano Felix Bertinato ◽  
Felipe Rebello Lourenço ◽  
Bruna Cipriano A Barros ◽  
...  
Keyword(s):  
Folic Acid ◽  
Mrna Expression ◽  
Hereditary Spherocytosis ◽  
Elevated Serum ◽  
Interferon Γ ◽  
Serum Folate ◽  
Total Serum ◽  
High Dose ◽  
Healthy Controls ◽  
Serum Concentrations

Patients with hereditary spherocytosis (HS) have increased rates of erythropoiesis and higher folate requirements. In a case-control study of patients with HS, we evaluated the associations between the use of 5 mg folic acid (FA) daily and serum concentrations of folate, unmetabolized folic acid (UMFA), interleukin (IL)-6, IL-8, IL-10, interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α); and mRNA expression of dihydrofolate reductase (DHFR), methylene tetrahydrofolate reductase (MTHFR), IL8, IFNG and TNFA genes. Total serum folate and folate forms were measured in 27 patients with HS (21 users [HS-U] and 6 non-users [HS-NU] of supplemental FA) and 54 healthy controls not consuming 5 mg/day supplemental FA. Each patient was matched to two controls based on age, sex and body mass index. The mononuclear leucocyte mRNA expression of relevant genes and their products were determined. Serum folate, UMFA, 5-methyl-tetrahydrofolate (5-methyl-THF) and tetrahydrofolate (THF) concentrations were significantly higher in HS-U compared with matched healthy controls (p<0.001, n=42). HS-NU had lower serum folate concentrations than matched healthy controls (p=0.044, n=12). HS-U and HS-NU presented similar hematological and biochemical markers profiles. No differences were found between HS-U and HS-NU for cytokine serum concentrations and mRNA expression genes. DHFR mRNA expression was higher in HS-U than in HS-NU. The use of high daily doses of FA for treatment of patients with HS may be excessive and is associated with elevated serum UMFA and elevated DHFR mRNA expression. It is not known whether long-term high-dose FA use by patients with HS might have adverse health effects.

scholarly journals Biomarkers of Folate and Vitamin B12 Are Related in Blood and Cerebrospinal Fluid

2007 ◽  
Vol 53 (2) ◽  
pp. 326-333 ◽  
Author(s):  
Rima Obeid ◽  
Panagiotis Kostopoulos ◽  
Jean-Pierre Knapp ◽  
Mariz Kasoha ◽  
George Becker ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Plasma Concentrations ◽  
B Vitamins ◽  
Serum Folate ◽  
Gas Chromatography Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Folate And Vitamin B12 ◽  
The Relationship

Abstract Background: B-vitamins (folate, B12) are important micronutrients for brain function and essential cofactors for homocysteine (HCY) metabolism. Increased HCY has been related to neurological and psychiatric disorders. We studied the role of the B-vitamins in HCY metabolism in the brain. Methods: We studied blood and cerebrospinal fluid (CSF) samples from 72 patients who underwent lumbar puncture. We measured HCY, methylmalonic acid (MMA), and cystathionine by gas chromatography-mass spectrometry; S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) by liquid chromatography-tandem mass spectrometry; and the B-vitamins by HPLC or immunoassays. Results: Concentrations were lower in CSF than serum or plasma for HCY (0.09 vs 9.4 μmol/L), SAH (13.2 vs 16.8 nmol/L), cystathionine (54 vs 329 nmol/L), and holotranscobalamin (16 vs 63 pmol/L), whereas concentrations in CSF were higher for MMA (359 vs 186 nmol/L) and SAM (270 vs 113 nmol/L; all P &lt;0.05). CSF concentrations of HCY correlated significantly with CSF folate (r = −0.46), CSF SAH (r = 0.48), CSF-albumin (r = 0.31), and age (r = 0.32). Aging was also associated with lower concentrations of CSF-folate and higher CSF-SAH. The relationship between serum and CSF folate depended on serum folate: the correlation (r) of serum and CSF-folate was 0.69 at serum folate &lt;15.7 nmol/L. CSF concentrations of MMA and holotranscobalamin were not significantly correlated. Conclusions: CSF and serum/plasma concentrations of vitamin biomarkers are significantly correlated. Older age is associated with higher CSF-HCY and CSF-SAH and lower CSF-folate. These metabolic alterations may be important indicators of low folate status, hyperhomocysteinemia, and neurodegenerative diseases.

scholarly journals The Effect of Bilateral Nephrectomy on Renalase and Catecholamines in Hemodialysis Patients

2021 ◽  
Vol 18 (12) ◽  
pp. 6282
Author(s):  
Magda Wiśniewska ◽  
Natalia Serwin ◽  
Violetta Dziedziejko ◽  
Małgorzata Marchelek-Myśliwiec ◽  
Barbara Dołęgowska ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Plasma Concentrations ◽  
Serum Levels ◽  
Hemodialysis Patients ◽  
Pressure Regulation ◽  
Serum Concentrations ◽  
Monoamine Oxidase Activity ◽  
Normal Kidney ◽  
Bilateral Nephrectomy ◽  
Arterial Blood

Background/Aims: Renalase is an enzyme with monoamine oxidase activity that metabolizes catecholamines; therefore, it has a significant influence on arterial blood pressure regulation and the development of cardiovascular diseases. Renalase is mainly produced in the kidneys. Nephrectomy and hemodialysis (HD) may alter the production and metabolism of renalase. The aim of this study was to examine the effect of bilateral nephrectomy on renalase levels in the serum and erythrocytes of hemodialysis patients. Methods: This study included 27 hemodialysis patients post-bilateral nephrectomy, 46 hemodialysis patients without nephrectomy but with chronic kidney disease and anuria and 30 healthy subjects with normal kidney function. Renalase levels in the serum and erythrocytes were measured using an ELISA kit. Results: Serum concentrations of renalase were significantly higher in post-bilateral nephrectomy patients when compared with those of control subjects (101.1 ± 65.5 vs. 19.6 ± 5.0; p < 0.01). Additionally, renalase concentrations, calculated per gram of hemoglobin, were significantly higher in patients after bilateral nephrectomy in comparison with those of healthy subjects (994.9 ± 345.5 vs. 697.6 ± 273.4, p = 0.015). There were no statistically significant differences in plasma concentrations of noradrenaline or adrenaline. In contrast, the concentration of dopamine was significantly lower in post-nephrectomy patients when compared with those of healthy subjects (116.8 ± 147.7 vs. 440.9 ± 343.2, p < 0.01). Conclusions: Increased serum levels of renalase in post-bilateral nephrectomy hemodialysis patients are likely related to production in extra-renal organs as a result of changes in the cardiovascular system and hypertension.

scholarly journals Gastrin and the Moderate Hypergastrinemias

2021 ◽  
Vol 22 (13) ◽  
pp. 6977
Author(s):  
Jens F. Rehfeld
Keyword(s):  
Helicobacter Pylori ◽  
Plasma Concentrations ◽  
Antral Mucosa ◽  
Serum Concentrations ◽  
Health Issues ◽  
Gastrin Secretion ◽  
Mucosal Growth ◽  
Early Phases ◽  
Molecular Nature ◽  
Concentration Levels

The antral hormone gastrin potently regulates gastric acid secretion and fundic mucosal growth. Consequently, appropriate gastrin secretion and plasma concentrations are important for the early phases of digestion. This review describes as the first premise the normal biogenesis of gastrin in the antral mucosa, but also mentions the extraantral expression. Subsequently, the molecular nature and concentration levels of gastrin in serum or plasma are overviewed. Third, assays for accurate measurements of plasma or serum concentrations are commented. Finally, the problem of moderate hypergastrinemia due to Helicobacter pylori infections and/or treatment with proton-pump inhibitors (PPI) is discussed. The review concludes that accurate measurement of the true concentrations of bioactive gastrins in plasma is important. Moreover, it suggests that moderate hypergastrinemias are also essential health issues that require serious attention.

Elevated plasma concentrations of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine in citrullinemia

Metabolism ◽  
2006 ◽  
Vol 55 (12) ◽  
pp. 1599-1603 ◽  
Author(s):  
Thomas Lücke ◽  
Dimitrios Tsikas ◽  
Nele Kanzelmeyer ◽  
Bernhard Vaske ◽  
Anibh Martin Das
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Asymmetric Dimethylarginine ◽  
Plasma Concentrations ◽  
Elevated Plasma ◽  
Nitric Oxide Synthase Inhibitor ◽  
Endogenous Nitric Oxide ◽  
Synthase Inhibitor ◽  
Oxide Synthase

Role of dimethylarginine dimethylaminohydrolase activity in regulation of tissue and plasma concentrations of asymmetric dimethylarginine in an animal model of prolonged critical illness

Metabolism ◽  
2012 ◽  
Vol 61 (4) ◽  
pp. 482-490 ◽  
Author(s):  
Mariska Davids ◽  
Milan C. Richir ◽  
Marlieke Visser ◽  
Björn Ellger ◽  
Greet van den Berghe ◽  
...  
Keyword(s):  
Animal Model ◽  
Critical Illness ◽  
Asymmetric Dimethylarginine ◽  
Plasma Concentrations ◽  
Dimethylarginine Dimethylaminohydrolase

scholarly journals Relationship of Oxidant and Antioxidant Markers to Asthma Severity in Egyptian Asthmatic Children

2017 ◽  
Vol 5 (5) ◽  
pp. 645-650 ◽  
Author(s):  
Inas R. EL-Alameey ◽  
Gihan A. Fathy ◽  
Mones M. Abu Shady ◽  
Alaa Ali ◽  
Hanan A. Fathy ◽  
...  
Keyword(s):  
Asymmetric Dimethylarginine ◽  
Strong Predictor ◽  
Predictive Biomarker ◽  
Asthma Severity ◽  
Healthy Children ◽  
Serum Concentrations ◽  
Asthmatic Children ◽  
Paraoxonase Activity ◽  
Asthmatic Patients ◽  
Anti Oxidant

BACKGROUND: Asthma is a chronic airway disease which is characterized by oxidant antioxidant imbalance with the generation of oxidative stress related mediators.AIM: The study aimed to evaluate the role of asymmetric dimethylarginine, and malondialdehyde as oxidant markers and serum paraoxonase activity as an antioxidant marker in asthma, and to determine their relationship to the asthma severity and lung function among asthmatic children in Egypt.PATIENTS AND METHODS: This case control study was conducted on sixty patients with asthma compared with sixty apparently healthy children of matched age and sex.RESULTS: Serum concentrations of oxidant markers as asymmetric dimethylarginine and malondialdehyde were significantly increased in asthmatic patients while anti-oxidant marker as paraoxonase activity was significantly decreased compared to healthy controls (P < 0.05). ANOVA test revealed highly significant elevation of the serum concentrations of oxidant markers while anti-oxidant marker was significantly decreased in severe asthmatic patients (P < 0.001) compared to the patients with moderate and mild asthma respectively. Serum malondialdehyde concentration was a strong predictor of asthma severity by multiple regression analysis (P < 0.05).CONCLUSION: The study revealed an imbalance between oxidative and antioxidant defence systems in asthmatic children. Serum concentration of malondialdehyde was the most predictive biomarker having a significant association with asthma severity.

scholarly journals The early changes in retinol-binding protein and prealbumin concentrations in plasma of protein–energy malnourished children after treatment with retinol and an improved diet

1980 ◽  
Vol 43 (3) ◽  
pp. 393-402 ◽  
Author(s):  
Suzanne Large ◽  
G. Neal ◽  
J. Glover ◽  
O. Thanangkul ◽  
R. E. Olson
Keyword(s):  
Body Weight ◽  
Vitamin A ◽  
Nutritional Status ◽  
Plasma Proteins ◽  
Binding Protein ◽  
Vitamin A Deficiency ◽  
Plasma Concentrations ◽  
Retinol Binding Protein ◽  
Mean Values ◽  
The Mean

1. Changes in total retinol-binding protein (RBP), the holoprotein (holoRBP) and prealbumin (PA) concentrations have been monitored in plasma of thirty protein- and vitamin A-deficient preschool children from within a few hours up to 7 weeks after treatment with retinol and a good-quality protein diet.2. The children were classified into groups according to nutritional status as having either kwashiorkor, marasmus-kwashiorkor or marasmus, and given formula diets whose protein and energy contents increased stepwise from 1 g and 105 kJ/kg body-weight respectively up to 4 g and 733 kJ/kg body-weight after 4 weeks. Retinol was administered in the forms of retinyl palmitate either orally or intramuscularly.3. PA and total RBP were determined by electroimmunoassay procedures and the holoRBP by its fluorescence after separation from other plasma proteins.4. RBP in plasma of the vitamin A-deficient child is largely denatured and incapable of binding administered retinol, which must first be taken up by the liver before native holoRBP is released. An increased pool of native apoprotein accumulates in the liver during vitamin A deficiency which is released into plasma quickly after retinol uptake to form peak concentrations of total and holoRBP approximately 3 h after dosing intramuscularly and 6 h orally.5. The accumulated pool of RBP was highest in livers from the marasmus group and lowest in those from the kwashiorkor group, reflecting their relative capacities to synthesize plasma proteins.6. The mean plasma concentrations of total and holoRBP for the various groups were minimal 24–48 h after dosing with retinol and then improved almost linearly over the following week.7. Mean plasma PA concentrations of the various groups on admission were also in order of the severity of their malnutrition. There was little or no change in this protein concentration over the first 24 h after dosing with retinol, but thereafter the mean values rose almost linearly over 2 weeks. Albumin on the other hand changed little during the first week. The results show that PA is the more sensitive measurement of protein nutritional status.

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