Antibacterial Activity of Phenyllactic acid Against Staphylococcus Epidermidis and Its Microbial Production: Modelling and Optimization-Based Analysis

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Jianxiong Ye ◽  
Yuxian Chen ◽  
Guanxuan Peng ◽  
Xinwei Yang ◽  
Jianzhong Huang ◽  
...  
Keyword(s):  
Experimental Data ◽  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Phenyllactic Acid ◽  
E Coli ◽  
Proposed Model ◽  
The Relationship ◽  
Modelling And Optimization ◽  
Production Modelling

Abstract Phenyllactic acid (PLA), an organic acid with extensive antimicrobial activity, is considered as a promising natural preservative to replace chemical preservatives. In order to study the inhibitory pattern of PLA, this paper established a novel mathematical model for the growth of Staphylococcus epidermidis under PLA inhibition. The simulated results showed that the relationship between the antimicrobial activity of PLA against S. epidermidis and its concentration was suitable to be represented by an exponential function. Based on the proposed model, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of PLA against S. epidermidis were evaluated. The computed results were found to match experimental data. The MBC value was found to be independent of the initial biomass of S. epidermidis from both the simulated results and experimental data, revealing that PLA was not consumed while killing the bacteria. Another kinetic model was established to describe the production of PLA by the engineered Escherichia coli. This model was then used to calculate the minimum biomass of E. coli to produce the MBC of PLA. The proposed models help to understand the inhibitory pattern of PLA, serving as a theoretical guide for the selection an appropriate strain to improve the product shelf-life.

scholarly journals ANTIMICROBIAL AND SYNERGISTIC ACTIVITY OF ESSENTIAL OILS FACING ISOLATED BACTERIA FROM SURGICAL STAFF’S

2019 ◽  
Vol 5 ◽  
Author(s):  
Tatiana Areas Cruz ◽  
Fernanda Rodrigues Torres ◽  
Monise Fazolin Petrucelli ◽  
Mariana Heinzen De Abreu ◽  
Silvia Sidneia Silva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Citrus Sinensis ◽  
Concentration Index ◽  
Inhibitory Concentration ◽  
Thymus Vulgaris ◽  
Synergistic Activity ◽  
Bacterial Strains ◽  
Microdilution Method

Objective:to evaluate antimicrobial and synergistic activity of essential oils (Citrus aurantifolia, Citrus sinensis, Mentha viridisand Thymus vulgaris) against isolated bacteria from surgical staff’s hands and bacterial strains. Method:the antimicrobial activity of oils was analyzed by microdilution method to determine the Minimum Inhibitory Concentration. Moreover, the combinatory effect of the oil that presented greater effectiveness using gentamycin sulphate through the Fractional Inhibitory Concentration index was tested. The study was performed over the period 2016-2018. Results:T. vulgaris was effective against Staphylococcus saprophyticus with a concentration of 0.0008 µL.mL-1and against the ATCC standard strains of Staphylococcus aureus, Salmonella choleraesuis,Staphylococcus epidermidis and Proteus vulgarisand the bacteria S. aureus, S. epidermidisand Bacillus sp. with MIC ≥ 0.10 µL.mL-1. This oil association with gentamycin sulphate showed synergistic activity againstS. epidermidis. Conclusion:T. vulgarisessential oil showed expressive antimicrobial activity against ATCC and isolated clinic microorganisms, suggesting broad spectrum of activity.

scholarly journals The antimicrobial activity of alcoholic extracts of leaves and bark from two varieties of ”Sangre de Drago” compared with the antimicrobial activity present in the latex of the same species

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1200
Author(s):  
Cecilia Barba Guevara ◽  
Luis Montaluisa ◽  
María Elena Maldonado Rodriguez
Keyword(s):  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Room Temperature ◽  
Medicinal Products ◽  
Bacterial Strains ◽  
Chemical Parameters ◽  
E Coli ◽  
Quality Control Standard ◽  
Physical And Chemical ◽  
Antimicrobial Effectiveness

Background: This research was carried out in order to study the antimicrobial effectiveness of crude latex of two varieties of "Sangre de Drago": Croton lechleri Muller Arg. and Croton urucurana Baill and compare that effectiveness to the antimicrobial activity of the alcoholic extracts of its leaves and bark.  Methods: The activity of the alcoholic extracts and latex were evaluated against bacterial strains of Staphylococcus epidermidis, Bacillus subtilis and Escherichia coli. The extraction of the alcoholic extracts (20% Tincture) of the leaves, bark and latex from the two Croton species was carried out by maceration using 70% alcohol as a menstruum, at room temperature, for 2 to 7 days, with shaking at least twice a day.  A 20% tincture was obtained, from which the physical and chemical parameters were determined as indicated by the Ecuadorian Quality Control Standard for natural medicinal products. Results: It was found that both the alcoholic extracts of the plant material and the crude latex indicate antimicrobial activity for S. epidermidis, moderate antimicrobial activity for B. subtilis and no antimicrobial activity for E. coli. The moderate antimicrobial activity against B. subtilis, at doses of 125 p.p.m., is in line with the findings of previous studies by other authors.  Conclusions: he antimicrobial activity of the latex of the two species against S. epidermidis is not registered in literature and, the negative antimicrobial activity for E. coli does not agree with what has been reported by previous studies.

Kinetic Model of Thermoelastic Martensite Transformation in Niti and NiMn Based Shape Memory Alloys

1995 ◽  
Vol 398 ◽  
Author(s):  
K. H. Wu ◽  
J.D. Shi ◽  
F. Yang ◽  
Z. J. Pu
Keyword(s):  
Experimental Data ◽  
Shape Memory Alloys ◽  
Shape Memory ◽  
Martensite Transformation ◽  
Quantitative Model ◽  
Proposed Model ◽  
Series Of Experiments ◽  
The Relationship ◽  
Kinetics Of ◽  
Good Agreement

ABSTRACTA new, quantitative model was developed to describe the martensite transformation kinetics of thermoelastic shape memory alloys (SMAs). In addition, a series of experiments were conducted to study the Kinetics of thermoelastic martensite transformation in four SMA systems: NiTi, NiTi-15at%Hf, NiTi-20at%Zr and NiMn-7.5at%Ti alloys. Comparisons between data of the kinetic of martensite transformation with the present theoretic models show that the proposed model is in good agreement and concurs with the experimental data. Also, a comparison of data from the proposed model with data from existing kinetic models, such as Liang's and Magee's [1,7], indicates that the proposed model can better describe the experimental data, including the relationship between dξ(T)/dT and ξ, and dξ(T)/dT and T.

Synthesis and Structures of Halo-Substituted Aroylhydrazones with Antimicrobial Activity

2012 ◽  
Vol 65 (4) ◽  
pp. 343 ◽  
Author(s):  
Mei Zhang ◽  
Dong-Mei Xian ◽  
Hai-Hua Li ◽  
Ji-Cai Zhang ◽  
Zhong-Lu You
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
X Ray Diffraction ◽  
X Ray ◽  
E Coli ◽  
Diphenyl Tetrazolium Bromide

A series of new halo-substituted aroylhydrazones have been prepared and structurally characterized by elemental analysis, 1H NMR, 13C NMR, and IR spectra, and single crystal X-ray diffraction. The compounds were evaluated for their antibacterial (Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas fluorescence) and antifungal (Candida albicans and Aspergillus niger) activities by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. Among the tested compounds, N′-(2-chloro-5-nitrobenzylidene)-2-fluorobenzohydrazide showed the most effective antimicrobial activity with minimum inhibitory concentration values of 0.82, 2.5, 1.7, 15.2, and 37.5 μg mL–1 against B. subtilis, S. aureus, E. coli, P. fluorescence, and C. albicans, respectively. The biological assay indicated that the presence of the electron-withdrawing groups in the aroylhydrazones improved their antimicrobial activities.

scholarly journals FORMULATION AND DEVELOPMENT OF TOPICAL ANTI ACNE FORMULATION OF SPIRULINA EXTRACT

2018 ◽  
Vol 10 (6) ◽  
pp. 229 ◽  
Author(s):  
Badduri Nihal ◽  
N. Vishal Gupta ◽  
D. V. Gowda ◽  
Manohar M.
Keyword(s):  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Water Soluble ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Drug Diffusion ◽  
Good Consistency ◽  
Agar Dilution ◽  
Dialysis Method

Objective: The objective of this research was to formulate and evaluate anti-acne ointment of C-phycocyanin(C-PC) extracted from spirulina.Methods: C-PC was successfully extracted from spirulina by using sonication and cold-maceration process and further purified by dialysis method. By employing disc diffusion and agar dilution method, antimicrobial activity and minimum inhibitory concentration(MIC) of C-PC as determined against Propionibacterium acne (P. acne) and Staphylococcus epidermidis(S. epidermidis). Further, the two different formulations were prepared by using water soluble and oleaginous bases, and the formulations were characterized for particle size, viscosity, pH, consistency, drug diffusion, antimicrobial activity, and antioxidant effect and stability studies.Results: C-PC showed MIC value of 1.5±0.1 mg/ml and 1.8±0.2 mg/ml against P. acne and S. epidermidis respectively. The developed formulation had a globule diameter of 5.44 mm, pH of 6.8±0.09, the viscosity of 175±0.2cps, spreadability of an 8.6±0.12g. cm/sec and had good consistency. Both formulations were found stable among which, formulation B(FB) had maximum drug content of 95±0.6% and drug release was up to 92±0.8%.Conclusion: The prepared topical C-PC ointment can be successfully employed in the treatment of acneagainstP. acne and S. epidermidis.

scholarly journals Optimization of Extraction of Phenolic Compounds with Antimicrobial Properties from Origanum vulgare

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1032
Author(s):  
Taja Žitek ◽  
Dragana Borjan ◽  
Andrej Golle ◽  
Željko Knez ◽  
Maša Knez
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Inhibitory Concentration ◽  
Optimal Conditions ◽  
Fluid Extraction ◽  
Origanum Vulgare ◽  
E Coli ◽  
Design Expert

Oregano (Origanum vulgare) is considered to be a good and cheap source of phenolic compounds with favorable biological activities, especially antimicrobial and antioxidant properties. Hypothesis/Purpose: The current work explored the optimization of the process conditions of solid–liquid extraction from Origanum vulgare to obtain extracts with high antimicrobial activity. We investigated which parameters promoted different efficiencies, leading to the maximum extraction of phenols and the consequent highest level of biological activity. Design-Expert Pro 11 was selected to design and analyze the experiments. The extracts were obtained by maceration as a simple method to recover value-added compounds from plant material, and supercritical fluid extraction was carried out as a green method with a high selectivity to obtain the compounds of interest. Pressure, temperature, and time were varied to obtain extracts with high antioxidant and antimicrobial activity. According to the results obtained using Design-Expert, the optimal conditions for maceration were at a temperature of 83 °C. The 1,1′-diphenyl-2-picrylhydrase method was used for the determination of antioxidant potential, while microdilution methods were used to determine the antimicrobial potential with regard to Staphylococcus aureus, Escherichia coli, and Candida albicans. A level of antioxidant activity of 87.21% was achieved. Supercritical fluid extracts showed higher antioxidant activity at a higher temperature of 60 °C and higher pressure of 25 MPa, although the results at 40 °C and 25 MPa were similar. The lowest minimum inhibitory concentration (MIC) values were 0.147 mg/mL for S. aureus, 0.728 mg/mL for E. coli, and 0.311 mg/mL for C. albicans. Overall, the optimal conditions for supercritical fluid extraction were 25 MPa and 40 °C. On the other hand, amounts of 0.208 mg/mL for S. aureus, 1.031 mg/mL for E. coli and 0.872 mg/mL for C. albicans were obtained using maceration. The MIC values of extracts obtained by supercritical fluid extraction were comparable to the minimum inhibitory concentration values obtained by different conventional techniques, such as those of Clevenger and Soxhlet.

scholarly journals Activated carbon from termite feces impregnated with silver: Preparation and evaluation of antimicrobial activity and ecotoxicity

2020 ◽  
Vol 17 (2) ◽  
pp. 1-24
Author(s):  
Giovanna Manesco ◽  
Carla Albertina Demarchi ◽  
Alexandre Bella Cruz ◽  
Jean Felipe Fossá Calisto ◽  
Rafael Martello ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Microbial Growth ◽  
Inhibitory Concentration ◽  
Chlorophyll Synthesis ◽  
Artemia Salina ◽  
Water Disinfection ◽  
E Coli ◽  
Seedling Germination ◽  
Kinetics Of ◽  
Preparation And Evaluation

Silver has a potent antimicrobial action, but its use is still restricted due to toxicity and aggregation problems. Impregnation with active carbon (AC) has been suggested as an alternative to solve this problem. In this study, termite feces were used to prepare composites containing silver nanoparticles and AC. Reducing agents and different amounts of H2 SO4 and silver were used. Antimicrobial activity was assessed using the minimum inhibitory concentration (MIC) and the kinetics of microbial growth of S. aureus, E. coli and C. albicans. Phytotoxicity and acute toxicity were assessed for Cucumis sativus and Artemia salina. The composites that presented MIC 500 μg/mL for S. aureus and E. coli were AC25Ag (6 μg/mL Ag) and AC25AgB (78 μg/mL Ag), respectively. AC6AgS showed antimicrobial activity in C. albicans with MIC of 500 μg/mL (23 μg/mL Ag). AC25AgB inhibited the growth of S. aureus and E. coli for 48 and 32 h, respectively. At a concentration of 10 g/L, the compounds showed deleterious effects on seedling germination and elongation and on the chlorophyll synthesis of C. sativus. The composites showed no toxicity to A. salina. Based on these results, AC25AgB has the potential to be used in water disinfection.

scholarly journals Evaluation in vitro of antimicrobial activity of tucumã oil (Astrocaryum Vulgare)

2019 ◽  
Vol 1 (1) ◽  
pp. 99-112 ◽  
Author(s):  
Aline Rossato ◽  
Larissa da Silva Silveira ◽  
Leonardo Quintana Soares Lopes ◽  
Walter Paixão De Sousa Filho ◽  
Larissa Finger Schaffer ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Major Health Problem ◽  
Major Health ◽  
Gram Positive Bacteria ◽  
Palm Trees ◽  
The Family

Hospital Infection is a major health problem and affects around 1.5 million people annually around the world. The Amazon region has a wide diversity of native palm trees that have fruits and oilseeds. Astrocaryum vulgare, commonly known as Tucumã in Brazil, belongs to the family Arecaceae. This palm has orange, fleshy, single-egg-shaped fruits that are used for therapeutic purposes in diseases of the eyes and skin due to the high content of carotenoids, oil is used in cooking, health treatment and massage. This study evaluated the antimicrobial activity of the Tucumã oil against 18 microorganisms. The antimicrobial activity of Tucumã was measured through the determination of the Minimum Inhibitory Concentration (MIC), as well as the determination of the Minimum Microbicidal Concentration (CMM) aiming to contribute to the discovery of new antimicrobials against pathogenic microorganisms’ human health and may contribute to the treatment of nosocomial infections. The results showed that the oil of Tucumã presented antimicrobial activity against five important bacteria, four Gram - positive bacteria (Enterococcus faecalis, Enterococcus faecium, Staphylococcus epidermidis and Streptococcus agalactiae) and one Gram - negative (Acinetobacter baumannii).

scholarly journals Polyphenolics profile effects upon the antioxidant and antimicrobial activity of propolis extracts

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mădălina Maria Nichitoi ◽  
Ana Maria Josceanu ◽  
Raluca Daniela Isopescu ◽  
Gabriela Olimpia Isopencu ◽  
Elisabeta-Irina Geana ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Diffusion Method ◽  
Type Model ◽  
E Coli ◽  
Antimicrobial Efficiency ◽  
Antioxidant And Antimicrobial Activity ◽  
Dpph Scavenging ◽  
Antibacterial And Antifungal ◽  
The Relationship

AbstractPropolis, a complex bee product, is a source of numerous bioactive principles, beneficial for human health, therefore it is intensively studied. In the present work, extracts of propolis from Bihor Romanian County were studied to identify the relationship between the polyphenolic derivatives profile and their antioxidant and antimicrobial activity. Extracts were obtained using water and 25%, 50%, and 70% ethanolic solutions (w/w), at 2:1, 4:1, and 6:1 liquid: solid ratios (w/w). 21 polyphenolic derivatives were quantified by UHPLC-MS, proving that the extracts composition strongly depends on the solvent. The sum of quantified polyphenolics extracted varied between 1.5 and 91.2 mg/g propolis. The antioxidant capacity was evaluated using the free radicals 2,2’-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging methods. Antimicrobial efficiency was tested against Gram-positive (B. subtilis), Gram-negative bacteria (E. coli), and fungi (C. albicans) by disc-diffusion method. All extracts, even the aqueous ones, demonstrated antibacterial and antifungal activity. Chemometric methods (partial least squares) and a saturation-type model were used to evaluate the contribution of various bioactive principles in building the antioxidant capacity of extracts. Both experimental and modelling results show that 50% ethanolic extracts provide a rich polyphenolics profile and ensure a good antioxidant capacity.

scholarly journals A PRELIMINARY STUDY ON ANTIMICROBIAL ACTIVITY OF Imperata cylindrica

2016 ◽  
Vol 1 (1) ◽  
pp. 63-66
Author(s):  
Ahmad Fahmi Harun Ismail ◽  
Othman Abd Samah ◽  
Abubakar Sule
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Imperata Cylindrica ◽  
Diffusion Test ◽  
E Coli ◽  
Polybutylene Succinate ◽  
Inhibition Zones ◽  
Preliminary Study ◽  
Mic Value

Antimicrobial properties of the methanol, chloroform or polybutylene succinate (PBS) extracts of leaves and rhizome from Imperata cylindrica were investigated against five clinical isolates of bacteria, namely Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. In disc diffusion test the three types of extracts showed varied levels of antimicrobial activity against the tested isolates, with inhibition zones ranging from 6.33 ± 0.58 to 11.67 ± 8.14 mm diameters. The highest activity was exerted by the methanol leaves extract on P. aeruginosa (11.67 ± 8.14mm) at 50 mg/ml and the least activity was exerted by both the leaves and rhizome extracts of the plant at 1mg/ml on B. subtilis, P. aeruginosa, S. aureus and E.coli (6.33 ± 0.58mm) respectively. A minimum inhibitory concentration (MIC) value of 25mg/ml was obtained for the methanol, chloroform, PBS leaves or rhizome extracts on B. subtilis, P. aeruginosa, S. aureus and E. coli

Sign in / Sign up

Export Citation Format

Share Document