Anti-ulcerogenic effect of the methanol extract of Chasmanthera dependens (Hochst) stem on male Wistar rats

2018 ◽  
Vol 29 (4) ◽  
pp. 377-383 ◽  
Author(s):  
Stephanie Abiola Tijani ◽  
Samuel B. Olaleye ◽  
Ebenezer O. Farombi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Gastric Ulcer ◽  
Gastric Mucosa ◽  
Antioxidant System ◽  
Methanol Extract ◽  
Histopathological Examination ◽  
Antioxidant Properties ◽  
Male Rats ◽  
Standard Drug

AbstractBackgroundOxidative stress and free radical-mediated processes have been implicated in the pathogenesis of indomethacin-induced gastric ulcer. This study investigated the ability of the methanol extract ofChasmanthera dependensto protect the gastric mucosal from oxidative damage induced by oral administration of indomethacin in rats.MethodsTheC. dependensstems were chopped into pieces, air-dried, and pulverized into powder. One kilogram of the powder was macerated in 1 L of methanol for 72 h. The mixture was filtered and evaporated using rotatory evaporator to obtain the extract ofC. dependens. Adult male rats were divided into eight groups of six animals per group and were pretreated orally with the methanol extract ofC. dependens(200, 400, and 800 mg/kg) or cimetidine (CIM), a standard drug (50 mg/kg), for 7 days. Gastric ulcer was induced orally with indomethacin. Ulcerogenic parameters, oxidative stress indices, and histopathological examination of the stomach were assessed to monitor the gastroprotective potential ofC. dependensstem.ResultsIndomethacin caused severe gastric mucosa damage and significant reduction in the gastric mucosa antioxidant system with concomitant increase in the level of lipid peroxidation. Pretreatment with the methanol extract ofC. dependensor CIM significantly reduced the formation of ulcer at the different doses administered. Similarly, pretreatments with the extract or CIM improved the antioxidant system, decreased acid output, lipid peroxidation, and improved the architecture of the gastric mucosa in ulcerated rats.ConclusionsThe results show the gastroprotective effect of the methanolic extract ofC. dependens, which may be attributed to its antioxidant properties.

scholarly journals Antioxidant properties of Ageratum conyzoides L. Asteraceae leaves

2018 ◽  
Vol 53 (4) ◽  
pp. 265-276 ◽  
Author(s):  
FO Adetuyi ◽  
KO Karigidi ◽  
ES Akintimehin ◽  
ON Adeyemo
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Erectile Dysfunction ◽  
Aqueous Extract ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Tissue Homogenate ◽  
Total Phenolic ◽  
Ageratum Conyzoides

In vitro antioxidative and anti-lipid peroxidative properties of aqueous and methanol extracts of Ageratum conyzoides leaves were studied in controlling erectile dysfunction caused by oxidative stress. Methanol extract gave a significantly (P ˂ 0.05) higher content of total phenolic (61.4 mgGAE/g), total flavonoid (42.2 mgQE/g), ascorbic (10.1 mgAAE/100g) and phosphomolybdate (45.8 mgAAE/g) than the aqueous extract. The result showed that the extracts have high antioxidant activities. However, the methanol extract showed a higher DPPH and hydrogen peroxide scavenging activities over aqueous extract but the aqueous extract had a higher reducing power. The methanol extract exhibited a greater inhibition against lipid peroxidation induced by Fe2+ in rat pancreas and penile tissue homogenate exemplified by their least IC50 (94.21 μg/ml in pancreas) and (75.95 μg/mL in penile tissue) while in rat brain homogenate the aqueous extract exhibited a greater inhibition against lipid peroxidation induced by Fe2+ with least IC50 of 91.74 μg/mL. Hence, these extracts can be used as a potent natural antioxidant against free radicals and as a natural source of combating erectile dysfunction caused by oxidative stress. The extracts of Ageratum conyzoides leaves could be useful therapeutically as erectogenic agent.Bangladesh J. Sci. Ind. Res.53(4), 265-276, 2018

scholarly journals EVALUATION OF ANTIOXIDANT PROPERTIES OF MEDGERM IN ACUTE SIMULATED HEPATITIS

2020 ◽  
Vol 20 (1) ◽  
pp. 59-66
Author(s):  
O. L. Tymchishin ◽  
V.V. Godovan ◽  
N.V. Kresyun
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant System ◽  
Liver Tissue ◽  
Toxic Hepatitis ◽  
Antioxidant Properties ◽  
Male Rats ◽  
Group I ◽  
Acute Toxic Hepatitis ◽  
Main Components ◽  
Acute Toxic

Disruption in redox processes plays a significant role in the pathogenesis of hepatitis. A balance between peroxidation, on the one hand, and the antioxidant system, on the other, is a prerequisite for maintaining normal cell activity. The modern arsenal of effective hepatoprotective drugs is not so wide therefore, the development of new hepatoprotectors is one of the topical issues in pharmacology. The purpose of the work is study the antioxidant properties of medgerma in acute experimental hepatitis. Material and methods. The experiments were performed on male rats weighing 180-220 g. Acute toxic hepatitis in rats was induced by intraperitoneal administration of D-galactosamine in a dosage of 400 mg / kg (LD50). The experimental animals were divided into 4 groups: group I (n = 10) included intact animals that were injected 0.9% sodium chloride solution (control group) intraperitoneally, II group (n = 40) involved the animals receiving only D-galactosamine; group III (n = 40) included rats, which received were administered Medmerg intraperitoneally in a dose of 0.4 mg / kg 7 days before D-galactosamine administration and 7 days after D-galactosamine administration. Group IV (n = 40) included rats, which received the comparison medicine, Essentiale® N, in at a dosage of 5 mg / kg in the same mode. The assessment of lipid peroxidation indices and the antioxidant system indices were carried out on days 1, 3, and 7 after the administration of hepatotoxin in the blood serum and the liver tissue homogenate supernatant. The condition of lipid peroxidation processes in the animals with galactosamine hepatitis was assessed by the content of lipid peroxidation end products in the blood and liver tissue that react with 2-thiobarbituric acid (TBA reactants). The condition of the main components of the antioxidant system was evaluated by the level of reduced glutathione and by the activity of catalase and superoxide dismutase. Results and discussion. It has been established that medgerm enhances the liver resistance to hepatotoxicant, as evidenced by a decrease in the level of lipid peroxidation products and the consumption of main components of the antioxidant system on the first day of hepatitis. Moreover, in the rats, which received Medgerm according to prevention scheme under acute toxic hepatitis, the course of the disease was milder than in the animals that did not receive this therapy. Conclusions and prospects for further research. Medgerm possesses hepatoprotective properties, which underlay positive effects on the state of lipid peroxidation and the main components of the antioxidant system.

Berberine nanoencapsulation attenuates hallmarks of scoplomine induced Alzheimer's-like disease in rats

2020 ◽  
Vol 15 ◽  
Author(s):  
Samar R. Saleh ◽  
Mariam M. Abady ◽  
Mohammed Nofal ◽  
Nashwa W. Yassa ◽  
Mohamed S. Abdel-latif ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Histopathological Examination ◽  
Memory Function ◽  
Amyloid Β ◽  
Active Pharmaceutical Ingredient ◽  
Isoquinoline Alkaloid ◽  
Drug Uptake ◽  
Male Rats ◽  
Morris Water Maze Test ◽  
Neuroprotective Effects

Background: Berberine (BBR), an isoquinoline alkaloid, acts as a multipotent active pharmaceutical ingredient to counteract several types of dementia based on its numerous pharmacological actions including antioxidant, antiinflammatory, cholesterol-lowering effect, and inhibition of Aβ production and AChE. However, BBR suffers from poor absorption, bioavailability and brain drug uptake. The present study is directed for the formulation and characterization of Chitosan BBR-nanoparticles (BBR-NPs) as well as the estimation of its neuroprotective effects against scopolamine induced cognitive impairments. Methods: BBR-NPs were formulated using ionic gelation method and tripolyphosphate was chosen as a cross linker. Nanoparticles size, zeta potential, encapsulation efficiency and releasing profile were estimated. To investigate the neuroprotective effects, adult fifty six Wistar male rats were randomly distributed into: three control groups, received saline, polyethylene glycol or chitosan- NPs respectively; induced group, received scopolamine (2 mg/ kg, i.p.) and three treated groups were orally administrated BBR (50 mg/ kg), BBR- NP (7 mg/ kg) and donepezil (2.25 mg/ kg, as positive control) followed by scopolamine injection after 40 min, daily for 4 weeks. Morris water maze test, oxidative stress parameters, cholinergic and amyloid-β processing intermediates as well as neuroplasticity markers and histopathological examination were assessed. Results: Our results showed that BBR- NPs were better than BBR and donepezil as BBR- NPs were powerful inhibitory ligands toward AChE and Aβ42 formation and significantly down regulated Tau, iNOS and BACE gene expression in rats’ hippocampus. BBR-NPs administration, at 1/6 of BBR therapeutic recommended dose, significantly improved learning and memory function. This could be accredited to the diminution of oxidative stress and amyloid-β toxicity in addition to the improvement of the neuroplasticity markers. Conclusions: The enhancing effect of BBR- NPs could be related to the enhancing of its bioavailability, absorption and brain drug uptake which need more investigation in future work.

Effect of Opuntia dejecta (Salm-Dyck) flowers in liver of fructose-fed rats: Biochemicals, enzymatic and histological studies

2021 ◽  
pp. 096032712110134
Author(s):  
O Zouaoui ◽  
K Adouni ◽  
A Jelled ◽  
A Thouri ◽  
A Ben Chrifa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Diabetes Type 2 ◽  
Male Rats ◽  
Control Group ◽  
Standard Drug ◽  
High Fructose ◽  
Group A ◽  
Histological Architecture

Phytochemical composition and antioxidant activity of flowers decoction at post-flowering stage (F3D) of Opuntia dejecta were determined. The obtained findings demonstrate that F3D has a marked antioxidant activity in all tested assays. Furthermore, the present study was designed to test the protective activity of F3D against induced Diabetes type 2 (DT2) in male rats. Those metabolic syndromes were induced by a high-fructose diet (HFD) (10% fructose solution) for a period of 20 weeks. F3D was administered orally (100 and 300 mg/kg body weight) daily for the last 4 weeks. Metformin (150 mg/kg body weight) was used as a standard drug and administrated orally for the last 4 weeks. The results showed a significant increase in blood glucose, triglycerides and hepatic markers (ALAT, ASAT and ALK-P) in HFD group. A significant increase in hepatic TBARS and a significant decrease in SOD, CAT and GPX were observed in fructose fed rats compared to control group. Administration of F3D showed a protective effect in biochemical and oxidative stress parameters measured in this study. Also, oral administration of F3D restored the histological architecture of rat liver in comparison with rats fed HFD. In conclusion, F3D attenuated hepatic oxidative stress in fructose-fed rats.

scholarly journals The Enhanced Beneficial Effect of Extra Virgin Olive Oil Over Evening Primrose Oil on Oxidative Stress, and Liver Function in Male Arthritic Rats

2021 ◽  
pp. 14-26
Author(s):  
Mohamed A. Kandeil ◽  
Sana’a O. Ebrahim ◽  
Basant M. Mahmoud
Keyword(s):  
Rheumatoid Arthritis ◽  
Oxidative Stress ◽  
Olive Oil ◽  
Liver Enzymes ◽  
Virgin Olive Oil ◽  
Antioxidant Properties ◽  
Extra Virgin Olive Oil ◽  
Male Rats ◽  
Evening Primrose Oil ◽  
Evening Primrose

Aims: Rheumatoid arthritis (RA) is characterized by the onset of oxidative stress. This study aimed to evaluate the enhancing of extra virgin olive (EVOO) and Evening primrose oil (EPO) on oxidative stress and liver enzymes in male Wistar rats and compare between them. Place and Duration: Faculty of Science biochemistry department, Between July 2018 and August 2018. Methodology: A Subcutaneous injection of 200 µl of Freund's complete adjuvant into a footpad of the right hind leg of Wistar male rats at two consecutive days induced RA. Rats received EVOO and EPO daily by oral gavage needle with gauge 18 at doses of 5 mg/kg b.wt./day. for 10 and 21 days. No loss was recorded in the experimental rats. Results: A significant depletion in serum Reduced glutathione content (GSH), glutathione peroxidase (GPX), and glutathione s transferase activities (GST) in arthritic rats compared to normal rats after 10 and 21 days of induction which improved significantly after 10 and 21 days of EPO and EVOO treatments. EPO and EVOO treatments for 21 days increased the GSH and GPX compared to 10 days treatments while no difference in GST activity. EVOO treatment improved GSH and GPX after 10 and 21 days than EPO treatment. The elevated uric acid levels in arthritic rats were markedly ameliorated as a result of EVOO and EPO treatment administration. Increased lipid peroxidation products (MDA), rheumatoid factor, and liver enzyme (Alanine transaminase ALT and Aspartate transaminase AST) were recorded in arthritic rats and they significantly progressed after EPO and EVOO treatments for 10 and 21 days but EVOO had the best effect at 21 days. Conclusion: EVOO and EPO showed significant antioxidant efficacies and improved affected liver enzymes due to rheumatoid arthritis onset. When comparing olive oil has more antioxidant properties than evening primrose oil, so we recommend more studies on olive oil combination with anti-arthritic medications to improve their efficacies with less toxicity.

scholarly journals Preclinical Study on the Ameliorating Effect of L-Ascorbic Acid for the Oxidative Stress of Caused by Chronic Administration of Organic Nitrates in Cardiovascular Diseases

2021 ◽  
Author(s):  
Ahmed M Hamdan ◽  
Zuhair M. Mohammedsaleh ◽  
Aalaa Aboelnour ◽  
Sherif M.H. Elkhannishi
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Chronic Administration ◽  
Stress Factors ◽  
Male Rats ◽  
Oxidative Stress Marker ◽  
Organic Nitrates ◽  
Dependent Manner ◽  
Dose Dependent

Abstract PurposeThe therapeutic activity of Glyceryl trinitrate (GTN) is mainly regulated by liberating nitric oxide (NO) and reactive nitrogen species (RNS). During this biotransformation, oxidative stress and lipid peroxidation inside the red blood cells (RBCs) occur. The principal objective of our research is to explain the ameliorating effect of L-ascorbic acid for the deleterious effects of chronic administration of nitrovasodilator drugs. MethodsWe studied some biochemical parameters for the oxidative stress using groups of high sucrose/fat (HSF) diet Wistar male rats chronically orally administered ISMN. Afterwards, we evaluated the role of L-ascorbic acid against these biochemical changes. ResultsChronic treatment with organic nitrates caused elevated serum levels of lipid peroxidation, hemoglobin derivatives as methemoglobin and carboxyhemoglobin, rate of hemoglobin autoxidation, the cellular levels of pro-inflammatory cytokines marker (NF-κB) and apoptosis markers (caspase-3) in myocardium muscles in a dose dependent manner. Meanwhile, such exposure caused decline in the enzymatic effect of superoxide dismutase (SOD), glutathione (GSH) and catalase activity (CAT) accompanied with a decrease of in the level of mitochondrial oxidative stress marker (nrf2) in myocardium muscles and decrease in the serum iron and total iron binding capacity (TIBC) in a dose dependent manner. Concomitant treatment with L-ascorbic acid significantly diminished these changes for all examined parameters.ConclusionChronic administration of organic nitrates leads to the alteration of the level of oxidative stress factors in the myocardium tissue due to generation of reactive oxygen species. Using vitamin C can effectively ameliorate such intoxication to overcome the nitrate tolerance.

scholarly journals Evaluation of Hepatoprotective and in vivo Antioxidant Potentials of Methanol Extract of Tephrosia pumila against Thioacetamide Induced Liver Toxicity in Wistar Rats

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Ramesh C ◽  
Pinkey Rawal ◽  
Soma Pramanik ◽  
Shabana S
Keyword(s):  
Biochemical Parameters ◽  
Methanol Extract ◽  
Liver Toxicity ◽  
Antioxidant Properties ◽  
Total Duration ◽  
Hepatoprotective Activity ◽  
Oral Toxicity ◽  
Standard Drug ◽  
Protein Ions

The objective of the current investigation was performed to assess the hepatoprotective potentials and in vivo antioxidant properties of methanol extract of Tephrosia pumila against thioacetamide induced liver damage in rats. The acute oral toxicity study of methanol extract was determined as per OECD guidelines and the extract was proved to be safe up to the dose of 2000mg/kg. The total duration of the study was 21 days and animals were divided into six groups. Hepatotoxicity was induced in the animals of all groups except normal control by single dose administration of Thioacetamide(100mg/kg) at first day of the study followed by animals were treated daily with standard drug sylimarin and methanol extract of Tephrosia pumila (100mg/kg, 200mg/kg and 400mg/kg) to respective groups for 21 days. Variations in biochemical parameters like alanine transferase (ALT), aspartate transferase (AST), alkaline phosphatase (ALP), total bilirubin, direct bilirubin, albumin, total protein, ions and others parameters like clotting time and weight of the liver were considered to determine beneficial effect of the extract. At the end of the study liver samples were collected and subjected to histopathological evaluation. There were significant variations in the above mentioned biochemical parameters in toxic control animals treated with Thioacetamide alone while in the animals treated with methanol extract and standard drug silymarin, all the parameters were normal possibly due to their beneficial property in protecting the liver against thioacetamide induced hepatotoxicity. The results obtained in the above study suggesting that, the methanol extract of Tephrosia pumila possess significant hepatoprotective activity.

Ameliorating Effect of Zinc Against Oxidative Stress and Lipid Peroxidation Induced by Fipronil in Male Rats

2016 ◽  
Vol 12 (1) ◽  
pp. 24-32 ◽  
Author(s):  
Eman S. Swelam ◽  
Ibrahim S. Abdallah ◽  
Abdel-Tawab H. Mossa
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Male Rats

Comparison of ameliorative effects of Taraxacum syriacum and N-acetylcysteine against acetaminophen-induced oxidative stress in rat liver and kidney

2020 ◽  
Author(s):  
Reza Eshrati ◽  
Mahvash Jafari ◽  
Saeed Gudarzi ◽  
Afshen Nazari ◽  
Esmaeil Samizadeh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Control Group ◽  
Standard Drug ◽  
Ameliorative Effect ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Serum Biochemical

Abstract Taraxacum syriacum (TS) with natural antioxidant and pharmacological activities may be considered for treatment of oxidative stress induced by acetaminophen (APAP). The aim of this study was to evaluate the ameliorative effects of the ethanol extract of TS root against hepatorenal toxicity induced by APAP in comparison to N-acetylcysteine (NAC) as a standard drug. Thirty male Wistar rats were randomly divided into five groups. Control group; APAP (1 g/kg) group; APAP–NAC (160 mg/kg) group and APAP-TS100 and APAP-TS200 groups: APAP plus 100 and 200 mg/kg of TS extract, respectively. After 7 days treatment, serum and liver and kidney tissues were prepared and evaluated. TS extract ameliorated the increased lipid peroxidation level and decreased antioxidant enzymes activities and glutathione level in liver and kidney of APAP-treated rats. Moreover, treatment with the TS extract caused significant reduction in the histopathological damages and high levels of serum biochemical markers of hepatic and renal functions after APAP treatment. This study suggests that the extract of TS roots has dose-dependent ameliorative effect against APAP-induced oxidative damage in liver and kidney due to its free radical scavenging and antioxidant properties. The overall efficacy of the extract at 200 mg/kg dose is comparable with NAC.

Evaluation of the Potential Cardioprotective Activity of Some Saudi Plants against Doxorubicin Toxicity

2012 ◽  
Vol 67 (5-6) ◽  
pp. 297-307 ◽  
Author(s):  
Osama M. Ashour ◽  
Ashraf B. Abdel-Naim ◽  
Hossam M. Abdallah ◽  
Ayman A. Nagy ◽  
Ahmed M. Mohamadin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activities ◽  
Histopathological Examination ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Serum Markers ◽  
Protective Effects ◽  
Medicinal Uses ◽  
Cordia Myxa

Doxorubicin (DOX) is an anthracycline antibiotic widely used as a chemotherapeutic agent in the treatment of several tumours. However, its cardiac toxicity limits its use at maximum therapeutic doses. Most studies implicated increased oxidative stress as the major determinant of DOX cardiotoxicity. The local Saudi flora is very rich in a variety of plants of quite known folkloric or traditional medicinal uses. Tribulus macropterus Boiss., Olea europaea L. subsp. africana (Mill.) P. S. Green, Tamarix aphylla (L.) H. Karst., Cynomorium coccineum L., Cordia myxa L., Calligonum comosum L’ Hér, and Withania somnifera (L.) Dunal are Saudi plants known to have antioxidant activities. The aim of the current study was to explore the potential protective effects of methanolic extracts of these seven Saudi plants against DOX-induced cardiotoxicity in rats. Two plants showed promising cardioprotective potential in the order Calligonum comosum > Cordia myxa. The two plant extracts showed potent in vitro radical scavenging and antioxidant properties. They significantly protected against DOX-induced alterations in cardiac oxidative stress markers (GSH and MDA) and cardiac serum markers (CK-MB and LDH activities). Additionally, histopathological examination indicated a protection against DOX-induced cardiotoxicity. In conclusion, C. comosum and C. myxa exerted protective activity against DOX-induced cardiotoxicity, which is, at least partly, due to their antioxidant effect

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