Exocrine Secretions of Wheel Bugs (Heteroptera: Reduviidae: Arilus spp.): Clarification and Chemistry

2013 ◽  
Vol 68 (11-12) ◽  
pp. 522-526
Author(s):  
Jeffrey R. Aldrich ◽  
Kamlesh R. Chauhan ◽  
Aijun Zhang ◽  
Paulo H. G. Zarbin
Keyword(s):  
Sex Pheromones ◽  
Methyl Eugenol ◽  
Hexanoic Acid ◽  
Bornyl Acetate ◽  
Rancid Odor ◽  
Caryophyllene Oxide ◽  
The North ◽  
Exocrine Secretions ◽  
Acetate Methyl ◽  
Species Specific

Wheel bugs (Heteroptera: Reduviidae: Harpactorinae: Arilus) are general predators, the females of which have reddish-orange subrectal glands (SGs) that are eversible like the osmeteria in some caterpillars. The rancid odor of Arilus and other reduviids actually comes from Brindley's glands, which in the North (A. cristatus) and South (A. carinatus) American wheel bugs studied emit similar blends of 2-methylpropanoic, butanoic, 3-methylbutanoic, and 2-methylbutanoic acids. The Arilus SG secretions studied here are absolutely species specific. The volatile SG components of A. carinatus include (E)-2-octenal, (E)-2-nonenal, (E)-2-decenal, (E,E)-2,4-nonadienal, (E)-2-undecenal, hexanoic acid, 4-oxo-nonanal, (E,E)-2,4-decadienal, (E,Z)-2,4- or (Z,E)-2,4-decadienal, and 4-oxo-(E)-2-nonenal; whereas in A. cristatus the SG secretion contains β-pinene, limonene, terpinolene, terpinen-4-ol, thymol methyl ether, α-terpineol, bornyl acetate, methyl eugenol, β-caryophyllene, caryophyllene oxide, and farnesol. Arilus spp. SG secretions may be sex pheromones, but verification of this hypothesis requires further testing.

Ultrastructural characteristics of spermatogenesis in three species of deep-sea hydrothermal vent mytilids

1997 ◽  
Vol 75 (2) ◽  
pp. 308-316 ◽  
Author(s):  
Marcel Le Pennec ◽  
Peter G. Beninger
Keyword(s):  
Deep Sea ◽  
Hydrothermal Vent ◽  
The North ◽  
Male Gametes ◽  
Bathymodiolus Thermophilus ◽  
Nutritional Biology ◽  
Protandric Hermaphroditism ◽  
Mid Atlantic Ridge ◽  
Ultrastructural Characteristics ◽  
Species Specific

To enhance our understanding of the reproductive biology of deep-sea hydrothermal vent mytilids, the histology of the male gonad and the ultrastructure of its gametes were studied in Bathymodiolus thermophilus, B. puteoserpentis, and B. elongatus. Specimens of B. thermophilus were collected at the 13°N site on the East Pacific ridge, while B. puteoserpentis were sampled from the Snake Pit site of the mid-Atlantic ridge and B. elongatus were obtained from the North Fiji Basin. Gonad histology conformed to the typical bivalve profile; the differences in the proportions of acinal and interacinal tissue, as well as differences in acinal fullness in B. puteoserpentis, indicate that gametogenesis is discontinuous in these deep-sea mytilids. Evidence of protandric hermaphroditism was observed in B. elongatus, which exhibited acini containing both maturing and residual male gametes and immature oocytes. The ultrastructural characteristics of the male gametes conform to those described for littoral bivalve species, and the spermatozoon is of the primitive type. No species-specific differences in spermatozoon ultrastructure were discerned. No evidence of bacterial inclusions was found in either the gametes or the associated gonad cells in any of the species examined. The male gametes are thus probably not vectors for the endosymbiotic bacteria that characterize the nutritional biology of the adults in this genus.

scholarly journals Phytochemical, cytotoxicity and antioxidant studies on the stem bark of Piper arborescens

2017 ◽  
Vol 13 (4) ◽  
pp. 840-845 ◽  
Author(s):  
Nicholas Anak Daniel ◽  
Fasihuddin Badruddin Ahmad ◽  
Zaini Assim ◽  
Chua Hun Pin
Keyword(s):  
Brine Shrimp ◽  
Antioxidant Activities ◽  
Stem Bark ◽  
Artemia Salina ◽  
Methyl Eugenol ◽  
Gas Chromatography Mass Spectrometry ◽  
Caryophyllene Oxide ◽  
Phytochemical Study ◽  
Crude Extracts ◽  
High Cytotoxicity

Phytochemical study on the stem bark of P. arborescens has isolated six secondary metabolites from the crude extracts by using column chromatography, while identification was performed by using Gas Chromatography - Mass Spectrometry (GC-MS), Nuclear Magnetic Resonance (NMR) and Fourier Transform Infrared (FTIR). The isolated metabolites were identified as caryophyllene oxide (1), α-bisabolol (2), benzamide 2-(methylamino) (3), 2-ethylpiperidine (4), piperine (5) and methyl eugenol (6). Toxicity test on the four crude extracts of P. arborescens shows a high cytotoxicity against Artemia salina brine shrimp with LC50 values ranging from 13.12 to 58.70 µg/mL. Greater cytotoxicity of the crude extracts of P. arborescens indicated the presence of potent cytotoxic components in this Piper spp. Antioxidant assay of P. arborescens against 2-diphenyl-1-picrylhydrazyl (DPPH) indicated moderate antioxidant activities of methanol, dichloromethane, chloroform and hexane crude extracts with EC50 values of 21.68, 23.82, 32.88 and 36.88 µg/mL, respectively.

Spatially explicit carrying capacity estimates to inform species specific recovery objectives: Grizzly bear (Ursus arctos) recovery in the North Cascades

2018 ◽  
Vol 222 ◽  
pp. 21-32 ◽  
Author(s):  
Andrea L. Lyons ◽  
William L. Gaines ◽  
Peter H. Singleton ◽  
Wayne F. Kasworm ◽  
Michael F. Proctor ◽  
...  
Keyword(s):  
Carrying Capacity ◽  
Ursus Arctos ◽  
Spatially Explicit ◽  
Grizzly Bear ◽  
North Cascades ◽  
The North ◽  
Species Specific ◽  
Capacity Estimates

scholarly journals Biotic and sedimentologic comparison of patch reefs on the north and south shelf of Belize, Central America

1992 ◽  
Vol 6 ◽  
pp. 45-45 ◽  
Author(s):  
C. D. Burke ◽  
S. J. Mazzullo
Keyword(s):  
Central America ◽  
Sea Level ◽  
Patch Reef ◽  
Acropora Palmata ◽  
The South ◽  
Northern Shelf ◽  
Patch Reefs ◽  
The North ◽  
Species Specific ◽  
Specific Growth Rates

Holocene patch-reefs occur throughout the shallow marine platform, to the lee of the barrier reef in northern and southern Belize, Central America. Patch reefs on the northern shelf that occur within an areally extensive patch reef complex (Mexico Rocks) indicate that differences exist between reefs here and well-studied patch reefs on the southern shelf that have been used by workers as a general model for patch reef development throughout Belize. This model proposes that patch reefs on the Belizean shelf are dominated by typical Atlantic-Caribbean, biotically-zoned coral assemblages of Acropora palmata and A. cervicornis that kept up or caught up with Holocene sea level rise during the last 8000 years to form large “keep up” or in some instances “catch up” reefs.In contrast to those in the south, the northern patch reefs are not biotically zoned, are dominated by Montastrea annularis rather than Acropora spp., and are much younger (400 years old) than those in the south. In addition, northern shelf patch reefs developed predominantly by lateral growth in a milieu of static sea level and are herein called “accretion” type reefs. These differences in biotic and sedimentologic parameters between reefs on the northern and southern shelves imply fundamentally different ecologic and sea level history controls on patch reef formation from north to south. A leading contributor to the variation among the reefs along the Belizean shelf may be species-specific growth rates of the coral species that initiate each patch reef, and response to sea level fluctuation versus stasis through time.

scholarly journals A rapid and inexpensive molecular technique to discriminate the north-eastern Atlantic and Mediterranean Atherina species and its potential applications in ecology and larval identification

Biologia ◽  
2012 ◽  
Vol 67 (5) ◽  
Author(s):  
Vítor Carvalho Almada ◽  
Sara Martins Francisco ◽  
Inęs Rosa ◽  
Vera Domingues ◽  
Henrique Cabral ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Molecular Technique ◽  
Formal Description ◽  
The North ◽  
North Eastern ◽  
Potential Applications ◽  
Species Specific ◽  
Specific Nucleotide

AbstractThis note describes a rapid and inexpensive Restriction Fragment Length Polymorphism technique to discriminate all species of Atherina (Pisces: Atherinidae) of the north-eastern Atlantic and the Mediterranean. This technique is based on digestion of a fragment of the 12S ribosomal RNA (12SrRNA) gene region of mitochondrial DNA with restriction enzymes that recognize species-specific nucleotide sites. The three currently recognized species in the area, as well as two additional forms awaiting formal description, can be discriminated using a set of four endonucleases. We argue that this simple and fast technique may be of great help in the identification of young stages and in ecological surveys.

scholarly journals Boron Oxide Nanoparticles Exhibit Minor, Species-Specific Acute Toxicity to North-Temperate and Amazonian Freshwater Fishes

2021 ◽  
Vol 9 ◽  
Author(s):  
Tyson J. MacCormack ◽  
Patrick T. Gormley ◽  
B. Ninh Khuong ◽  
Olivia A. Adams ◽  
Susana Braz-Mota ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Toxicity ◽  
Boron Oxide ◽  
Model Systems ◽  
Oxide Nanoparticles ◽  
Frequency Distributions ◽  
Aerobic Activity ◽  
The North ◽  
Aquatic Vertebrates ◽  
Species Specific

Boron oxide nanoparticles (nB2O3) are manufactured for structural, propellant, and clinical applications and also form spontaneously through the degradation of bulk boron compounds. Bulk boron is not toxic to vertebrates but the distinctive properties of its nanostructured equivalent may alter its biocompatibility. Few studies have addressed this possibility, thus our goal was to gain an initial understanding of the potential acute toxicity of nB2O3 to freshwater fish and we used a variety of model systems to achieve this. Bioactivity was investigated in rainbow trout (Oncorhynchus mykiss) hepatocytes and at the whole animal level in three other North and South American fish species using indicators of aerobic metabolism, behavior, oxidative stress, neurotoxicity, and ionoregulation. nB2O3 reduced O. mykiss hepatocyte oxygen consumption (ṀO2) by 35% at high doses but whole animal ṀO2 was not affected in any species. Spontaneous activity was assessed using ṀO2 frequency distribution plots from live fish. nB2O3 increased the frequency of high ṀO2 events in the Amazonian fish Paracheirodon axelrodi, suggesting exposure enhanced spontaneous aerobic activity. ṀO2 frequency distributions were not affected in the other species examined. Liver lactate accumulation and significant changes in cardiac acetylcholinesterase and gill Na+/K+-ATPase activity were noted in the north-temperate Fundulus diaphanus exposed to nB2O3, but not in the Amazonian Apistogramma agassizii or P. axelrodi. nB2O3 did not induce oxidative stress in any of the species studied. Overall, nB2O3 exhibited modest, species-specific bioactivity but only at doses exceeding predicted environmental relevance. Chronic, low dose exposure studies are required for confirmation, but our data suggest that, like bulk boron, nB2O3 is relatively non-toxic to aquatic vertebrates and thus represents a promising formulation for further development.

scholarly journals Chromosome-level genome assembly of a benthic associated Syngnathiformes species: the common dragonet, Callionymus lyra

Gigabyte ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Sven Winter ◽  
Stefan Prost ◽  
Jordi de Raad ◽  
Raphael T. F. Coimbra ◽  
Magnus Wolf ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Morphological Differentiation ◽  
Chromosome Length ◽  
Morphological Transformation ◽  
High Quality ◽  
The North ◽  
Repeat Content ◽  
The Common ◽  
Species Specific ◽  
Chromosome Level

Background The common dragonet, Callionymus lyra, is one of three Callionymus species inhabiting the North Sea. All three species show strong sexual dimorphism. The males show strong morphological differentiation, e.g., species-specific colouration and size relations, while the females of different species have few distinguishing characters. Callionymus belongs to the ‘benthic associated clade’ of the order Syngnathiformes. The ‘benthic associated clade’ so far is not represented by genome data and serves as an important outgroup to understand the morphological transformation in ‘long-snouted’ syngnatiformes such as seahorses and pipefishes. Findings Here, we present the chromosome-level genome assembly of C. lyra. We applied Oxford Nanopore Technologies’ long-read sequencing, short-read DNBseq, and proximity-ligation-based scaffolding to generate a high-quality genome assembly. The resulting assembly has a contig N50 of 2.2 Mbp and a scaffold N50 of 26.7 Mbp. The total assembly length is 568.7 Mbp, of which over 538 Mbp were scaffolded into 19 chromosome-length scaffolds. The identification of 94.5% complete BUSCO genes indicates high assembly completeness. Additionally, we sequenced and assembled a multi-tissue transcriptome with a total length of 255.5 Mbp that was used to aid the annotation of the genome assembly. The annotation resulted in 19,849 annotated transcripts and identified a repeat content of 27.7%. Conclusions The chromosome-level assembly of C. lyra provides a high-quality reference genome for future population genomic, phylogenomic, and phylogeographic analyses.

scholarly journals Antioxidant Activity and Chemical Composition of Essential Oils of some Aromatic and Medicinal Plants from Albania

2017 ◽  
Vol 12 (5) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Entela Hodaj-Çeliku ◽  
Olga Tsiftsoglou ◽  
Lulëzim Shuka ◽  
Sokol Abazi ◽  
Dimitra Hadjipavlou-Litina ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Essential Oils ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stable Radical ◽  
In Vitro Assays ◽  
Bornyl Acetate ◽  
Soybean Lipoxygenase ◽  
Chemical Compositions ◽  
Caryophyllene Oxide

The chemical compositions have been investigated of the volatile oils of nine populations of six species from Albania, namely Artemisia absinthium, Calamintha nepeta, Hypericum perforatum, Sideritis raeseri subsp. raeseri, Origanum vulgare subsp. hirtum from two wild populations, and Salvia officinalis (sage) from two wild and one cultivated population,. The essential oils were obtained by hydrodistillation and their analyses were performed by GC–MS. The major constituents were: A. absinthium: neryl isovalerate (19.5%), geranyl isobutanoate (16.4%) and carvacrol (8.8%); C. nepeta: pulegone (31.7%), spathulenol (20.0%) and isomenthone (12.7%); H. perforatum: caryophyllene oxide (31.0%), δ-selinene (10.5%) and carvacrol (10.4%); O. vulgare: carvacrol (81.0, 78.6%), γ-terpinene (5.5, 7.1%) and p-cymene (4.9, 4.1%) for O. vulgare originating from Tepelena and Vlora, respectively; S. raeseri: carvacrol (36.7%), caryophyllene oxide (17.8%), β-caryphyllene (8.7%), spathulenol (7.7%) and myrtenol (6.4%); S. officinalis: camphor (40.2, 47.8, 45.9%), α-thujone (19.2, 22.2, 13.7%), eucalyptol (5.4, 2.6, 6.0%), camphene (5.8, 6.1, 3.9, %), borneol (2.1, 2.9, 5.7%) and bornyl acetate (3.3, 1.4, 5.6%) for samples originating from Tepelena, Tirana and Vlora, respectively. The essential oils were also tested for their free radical scavenging activity using the following in vitro assays: i) interaction with the free stable radical of DPPH (1,1-diphenyl-2-picrylhydrazyl), and ii) inhibition of linoleic acid peroxidation with 2,2'-azobis-2-methyl-propanimidamide, dihydrochloride (AAPH). Finally, their inhibitory activity toward soybean lipoxygenase was evaluated, using linoleic acid as substrate. The essential oil of O. vulgare (OV-VL) presented the highest interaction with the stable radical DPPH (76.5%), followed by that of A. absinthium (54.7%). O. vulgare (OV-TP) and A. absinthium showed high anti-lipid peroxidation activity, 97.5% and 96.5%, respectively, higher than that of the reference compound trolox (73.0%). Only the tested sample of O. vulgare (OV-VL) significantly inhibited soybean lipoxygenase (54.2%).

scholarly journals Characterisation of Vibrio Species from Surface and Drinking Water Sources and Assessment of Biocontrol Potentials of Their Bacteriophages

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Mpho Defney Maje ◽  
Christ Donald Kaptchouang Tchatchouang ◽  
Madira Coutlyne Manganyi ◽  
Justine Fri ◽  
Collins Njie Ateba
Keyword(s):  
Water Samples ◽  
Biofilm Formation ◽  
Tap Water ◽  
Multidrug Resistant ◽  
Biochemical Tests ◽  
Antibiotic Resistant ◽  
North West ◽  
The North ◽  
C 50 ◽  
Species Specific

The aim of this study was to characterise Vibrio species of water samples collected from taps, boreholes, and dams in the North West province, South Africa, and assess biocontrol potentials of their bacteriophages. Fifty-seven putative Vibrio isolates were obtained on thiosulfate-citrate-bile-salt-sucrose agar and identified using biochemical tests and species-specific PCRs. Isolates were further characterised based on the presence of virulence factors, susceptibility to eleven antibiotics, and biofilm formation potentials. Twenty-two (38.60%) isolates were confirmed as Vibrio species, comprising V. harveyi (45.5%, n = 10), V. parahaemolyticus (22.7%, n = 5), V. cholerae (13.6%, n = 3), V. mimicus (9.1%, n = 2), and V. vulnificus (9.1%, n = 2). Three of the six virulent genes screened were positively amplified; four V. parahaemolyticus possessed the tdh (18.18%) and trh (18.18%) genes, while the zot gene was harboured by 3 V. cholerae (13.64%) and one V. mimicus (4.55%) isolate. Isolates revealed high levels of resistance to cephalothin (95.45%), ampicillin (77.27%), and streptomycin (40.91%), while lower resistances (4.55%–27.27%) were recorded for other antimicrobials. Sixteen (72.7%) isolates displayed multiple antibiotic-resistant properties. Cluster analysis of antibiotic resistance revealed a closer relationship between Vibrio isolates from different sampling sites. The Vibrio species displayed biofilm formation potentials at 37°C (63.6, n = 14), 35°C (50%, n = 11), and 25°C (36.4%, n = 8). Two phages isolated in this study (vB_VpM_SA3V and vB_VcM_SA3V) were classified as belonging to the family Myoviridae based on electron microscopy. These were able to lyse multidrug-resistant V. parahaemolyticus and V. cholerae strains. These findings not only indicate the presence of antibiotic-resistant virulent Vibrio species from dam, borehole, and tap water samples that could pose a health risk to humans who either come in contact with or consume water but also present these lytic phages as alternative agents that can be exploited for biological control of these pathogenic strains.

Terrestrial vegetation monitoring at Cape Hatteras National Seashore: 2019 data summary

2022 ◽  
Author(s):  
Maxwell Boyle ◽  
Elizabeth Rico
Keyword(s):  
North Carolina ◽  
Loblolly Pine ◽  
Terrestrial Vegetation ◽  
Vegetation Monitoring ◽  
The North ◽  
Cape Hatteras ◽  
Monitoring Effort ◽  
Cape Hatteras National Seashore ◽  
Species Specific ◽  
National Seashore

The Southeast Coast Network (SECN) conducts long-term terrestrial vegetation monitoring as part of the nationwide Inventory and Monitoring Program of the National Park Service (NPS). The vegetation community vital sign is one of the primary-tier resources identified by SECN park managers, and monitoring is currently conducted at 15 network parks (DeVivo et al. 2008). Monitoring plants and their associated communities over time allows for targeted understanding of ecosystems within the SECN geography, which provides managers information about the degree of change within their parks’ natural vegetation. The first year of conducting this monitoring effort at four SECN parks, including 52 plots on Cape Hatteras National Seashore (CAHA), was 2019. Twelve vegetation plots were established at Cape Hatteras NS in July and August. Data collected in each plot included species richness across multiple spatial scales, species-specific cover and constancy, species-specific woody stem seedling/sapling counts and adult tree (greater than 10 centimeters [3.9 inches {in}]) diameter at breast height (DBH), overall tree health, landform, soil, observed disturbance, and woody biomass (i.e., fuel load) estimates. This report summarizes the baseline (year 1) terrestrial vegetation data collected at Cape Hatteras National Seashore in 2019. Data were stratified across four dominant broadly defined habitats within the park (Maritime Tidal Wetlands, Maritime Nontidal Wetlands, Maritime Open Uplands, and Maritime Upland Forests and Shrublands) and four land parcels (Bodie Island, Buxton, Hatteras Island, and Ocracoke Island). Noteworthy findings include: A total of 265 vascular plant taxa (species or lower) were observed across 52 vegetation plots, including 13 species not previously documented within the park. The most frequently encountered species in each broadly defined habitat included: Maritime Tidal Wetlands: saltmeadow cordgrass Spartina patens), swallow-wort (Pattalias palustre), and marsh fimbry (Fimbristylis castanea) Maritime Nontidal Wetlands: common wax-myrtle (Morella cerifera), saltmeadow cordgrass, eastern poison ivy (Toxicodendron radicans var. radicans), and saw greenbriar (Smilax bona-nox) Maritime Open Uplands: sea oats (Uniola paniculata), dune camphorweed (Heterotheca subaxillaris), and seabeach evening-primrose (Oenothera humifusa) Maritime Upland Forests and Shrublands: : loblolly pine (Pinus taeda), southern/eastern red cedar (Juniperus silicicola + virginiana), common wax-myrtle, and live oak (Quercus virginiana). Five invasive species identified as either a Severe Threat (Rank 1) or Significant Threat (Rank 2) to native plants by the North Carolina Native Plant Society (Buchanan 2010) were found during this monitoring effort. These species (and their overall frequency of occurrence within all plots) included: alligatorweed (Alternanthera philoxeroides; 2%), Japanese honeysuckle (Lonicera japonica; 10%), Japanese stilt-grass (Microstegium vimineum; 2%), European common reed (Phragmites australis; 8%), and common chickweed (Stellaria media; 2%). Eighteen rare species tracked by the North Carolina Natural Heritage Program (Robinson 2018) were found during this monitoring effort, including two species—cypress panicgrass (Dichanthelium caerulescens) and Gulf Coast spikerush (Eleocharis cellulosa)—listed as State Endangered by the Plant Conservation Program of the North Carolina Department of Agriculture and Consumer Services (NCPCP 2010). Southern/eastern red cedar was a dominant species within the tree stratum of both Maritime Nontidal Wetland and Maritime Upland Forest and Shrubland habitat types. Other dominant tree species within CAHA forests included loblolly pine, live oak, and Darlington oak (Quercus hemisphaerica). One hundred percent of the live swamp bay (Persea palustris) trees measured in these plots were experiencing declining vigor and observed with symptoms like those caused by laurel wilt......less

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