Determination of Galendo’s Shelf Life on Various of Storage Temperatures by Arrhenius Method

This study aims to estimate the shelf life by applying the Arrhenius formula model to "galendo" which is vacuum packed and stored at various temperatures. Estimation of the shelf-life limit of galendos was carried out by measuring the peroxide number and sensory values ​​of galendos which were deliberately kept open without being packed at room temperature (25 ○C). The sensory values ​​used as the threshold for consumer rejection of the level of galendo's rancid odor were achieved on the 27th day of storage and the current peroxide count value was 42.2724 meq/kg. Galendo which was vacuum packed with aluminum foil and stored at various temperatures of 15 ○C, 25 ○C, and 35 ○C, then the shelf life was determined using the Arrhenius and Q10 models based on the parameters of the total peroxide number (TPN) and the total microbial content. Based on the experiment in this study, the estimation results of galendo's shelf life based on the total peroxide number (TPN) test parameter show that storage at 15 ○C has a shelf life of 83 days with a deterioration rate (k) of 0.0207, storage at 25 ○C shelf life 67 days with a deterioration rate (k) 0.0247, and storage at a temperature of 35 ○C for 58 days with a deterioration rate (k) 0.0286. The rate of quality degradation with a value of Q10 (15 oC - 25 oC) of 1.23 and a value of Q10 (25 oC - 35 oC) of 1.16. The higher the storage temperature, the higher the microorganism content.

Dissemination of the oil draining spinner on the small business of oyster mushroom chips in Meteseh Village, Semarang City

The high oil content in fried products, including mushroom chips, can cause low quality chips. Chips contain a lot of oil which causes easy oxidation which can cause a rancid odor. This service aims to improve the quality of chips through the application of an oil draining spinner. The application of this machine is intended to reduce the maximum oil content in the oyster mushroom chips. The implementation stage begins with providing partners with a complete understanding of the tool. Furthermore, the engine performance test was carried out in the oil draining process, and the evaluation of the draining results is carried out. The results show that partners understand very well the parts, the function, and the working principle of the machine. Draining using the machine can effectively reduce the oil content of the chips, although a few chips were found to be damaged due to friction or impact with the machine wall. The draining capacity and resistance of the chips to not go rancid can be increased.

Malignant Edema in Horse by Clostridium perfringens Type A

Background: Malignant edema is one of the terms used to designate severe necrotizing syndromes in soft tissues by Clostridium spp. which are potentially fatal in farm animals. These species are responsible for myonecrosis, belonging to the group of histotoxic clostridia, and may also culminate in toxemia with the worsening of the lesions. These clostridia and their spores require a gateway such as wounds on mucous membranes or skin, which may occur due to shear, tail cut, injuries during delivery, castration or injections by contaminated needles. This report aims to describe the clinical-pathological findings of a case of malignant edema caused by C. perfringens type A in an equine.Case: A female equine, undefined breed, used as traction animal, had a history of abdominal pain. According to the requisitioning veterinary, the tutor reused needles for medication. On palpation, a compact mass was noticed in the pelvic flexure, as well as edema on the region of head and neck with crackling areas. After surgical intervention for compactation correction, the animal did not show anesthetic recovery and was submited to euthanasia. Tissue samples were collected, fixed in 10% buffered formalin solution, routinely processed for histopathology and stained with hematoxylin and eosin and Gram stain. Samples of serous-sanguineous edema fluid and fragments of the abdominal muscles and neck were collected. The samples were kept under refrigeration and sent for microbiological culture. Necropsy showed the subcutaneous region of the pectoral was markedly gelatinous and yellowish (edema) and subcutaneous emphysema characterized by accumulation of serous-sanguineous fluid and gas bubbles. In microscopy, we verified fibrous-haemorrhagic, emphysematous, suppurative and diffuse superficial histiocytic necrotizing cellulitis and myositis. Gram-positive bacillary aggregates were observed in spleen and subcutaneous sections. Colony suggestive of Clostridium perfringens were submitted to PCR for confirmation of identity followed by genotyping. Lastly, C. perfringens type A was isolated from the muscle fragments and serosanguinolent liquid collected.Discussion: In the present study, the diagnosis of malignant edema caused by Clostridium perfringens type A in an equine was confirmed by the clinical-pathological findings added to the isolation and genotyping of the agent from fragments of injured muscle. Although C. perfringens has been reported in cases of clostridial myositis in horses in the United States, in Brazil there are only reports of sporadic cases associated with C. septicum, C. novyi and C. chauvoei in this species. In histotoxic cases by C. perfringens, alpha toxin is known to be the main virulence factor involved, causing destruction of the phospholipid membrane of erythrocytes, endothelial cells, leukocytes and muscle fibers. As consequence, there is an increase in the vascular permeability of capillaries and, with the spread of the infection, there is production of inflammatory edema with serosanguineous exudate and gas. Typically, anatomopathological findings are characterized by increased volume of the affected region associated with edema with bloody fluid and rancid odor, gas bubbles in the subcutaneous tissue, and fasciae associated or not with necrotic myositis. It is assumed that a contaminated needle injection, performed during the treatment of colic symptoms on the property has been the gateway to infection. The clinical course of clostridial infection in horses is considered acute, ranging from 24 to 48 h. In this case, was observed the animal with signs of cervical and pectoral edema still in the property, a day before euthanasia. This is the first study to confirm Clostridium perfringens as a cause of gas gangrene in horses in Brazil.

Physical-Chemical Properties of Poultry Rendering Meal of Whole Broiler Carcasses Stored for Different Times and Temperatures

The effect of storage temperature on the physical-chemical properties of poultry rendering meals (PRM) of whole broiler carcasses was evaluated. Chemical parameters, oxidation and biogenic amines (BA) profiles were determined. A completely randomized design in a 2x4 factorial arrangement (two stored temperature: 26 ºC or 7 ºC; and four storage times: 0, 24, 48 and 72h before rendering) was used. PRM nutrient levels were within the acceptable ranges determined for animal byproducts in all treatments and they did not influence PRM acidity index or TBARS (P>0.05). The peroxide index of all PRM was higher than the acceptable limit (>10 meq/1000g) but did not cause a rancid odor. Lower total BA content was determined at 72 h of storage in PRMs from refrigerated carcasses. The nutrient composition of PRMs shows quality enough to possibly be utilized as a feedstuff. Additional studies are needed to establish the safety of these byproducts as feedstuffs. Keywords: biogenic amines, byproduct, feedstuff, nutritional composition

Teknologi Tepat Guna Pemurnian Minyak Kelapa Tradisional Di Desa Mengkalang Jambu Kabupaten Kubu Raya Kalimantan Barat

Traditional coconut oil has the potential to be developed into commercial oil. These community service activities are a) coconut oil production practices, b) introduction of coconut oil refining technology and c) assessment of people's perceptions of coconut oil production. The variables used are the process of making oil, product quality, technology transfer and people's motivation to make oil. Questionnaire data were analyzed with contingencies to show the close relationship between variables and community groups. The results of the activity showed that there were two ways of making traditional coconut oil, using fresh coconut milk and fermented coconut milk. The resulting oil is crude coconut oil with a characteristic rancid odor, dark color and not durable. The process of oil production is a variable that shows real differences between young and old groups, while other variables are not significantly different. As many as 75% of young group respondents stated that the process of making coconut oil at a somewhat difficult to difficult level. The close relationship between the respondent group and the process of making coconut oil has high accuracy, which is 0.64. Improving coconut oil manufacturing techniques with the refining process can be one way to improve quality and efforts to support the commercialization of traditional coconut oil.

Modeling the Effect of the Oxidation Status of the Ingredient Oil on Stability and Shelf Life of Low-Moisture Bakery Products: The Case Study of Crackers

In packed low-moisture foods such as crackers, oxidation is generally the main cause of quality depletion during storage. It is commonly believed, but scarcely investigated, that product shelf life depends on the oxidative status of the lipid ingredients. In this study, the influence of oxidation degree of the ingredient sunflower oil on cracker oxidative stability and hence shelf life was investigated. To this aim, oil with increasing peroxide values (PVs) (5, 11, and 25 mEqO2/kgoil) was used to prepare crackers. Just after production, crackers presented similar peroxide and rancid odor intensity, probably due to the interactive pathways of oxidative and Maillard reactions. Crackers were packed and analyzed for PV and rancid odor during storage at 20, 40, and 60 °C. Rancid odor well discriminated cracker oxidative status. Relevant oxidation rates were used to develop a shelf life predictive model based on the peroxide value of the ingredient oil. It was estimated that an oil PV from 5 to 15 mEqO2/kgoil shortens cracker Shelf Life (SL) by 50%, independently of storage temperature. These results demonstrate the critical impact of ingredient quality on product performance on the market.

Pathological and clinical investigations of an outbreak of Blackleg disease due to C. chauvoei in cattle in Punjab, Pakistan

Introduction: Clostridium chauvoei (C. chauvoei) is an anaerobic, histotoxic Gram-positive, bacterium causing fatal myonecrosis in livestock with high mortalities. The disease is common in dairy animals, but little is known about the pathophysiology of the disease in exotic (non-native) animals kept under local conditions in Pakistan. Methodology: Diagnosis of blackleg was made based on hematological and serum biochemical analysis, PCR, necropsy and histopathology. Results: Clinically sick animals exhibited fever, lameness, subcutaneous gaseous swelling and edema particularly in hindquarter and front legs. Hematological analysis showed increases in erythrocyte sedimentation rate and reduces in number of red blood cells, packed cell volume, leukocytes and differential leukocyte count. Serum aspartate aminotransferase, lactate dehydrogenase, alkaline phosphates, alanine aminotransferase, urea, creatinine, creatine kinase, and creatinine phosphokinase were significantly (P < 0.05) higher in the infected animals. At necropsy, swelling areas contained straw-colored fluid with gas bubbles. The muscles were swollen, dark to black and exhibited crepitation sounds at the time of incisions with a rancid odor. Severe pulmonary edema, myocarditis along with petechial hemorrhages, as well as enlargement and congestion of liver and spleen have been observed. Microscopic examination revealed severe inflammatory reaction, edema, and disruption of the myofibrils. Examination of heart, spleen, liver, kidneys, intestine, and lungs showed congestion, severe inflammatory changes with neutrophilic infiltration and necrosis accompanied by dissociation of the normal tissue structure. PCR confirmed C. chauvoei in exudates and different samples of muscles. Conclusion: The pathophysiology should be considered in diagnosis of blackleg. The disease is exist in the non-native cattle farms and biosecurity measures have to be elevated.

Kajian Sistem Pengolahan Minyak Kelapa Murni (Virgin Coconut Oil) dengan Metode Pemanasan

Pure coconut oil or Virgin coconut oil is a modification of making coconut oil to produce products with the lower water and acid level , clear and has a long shelf life of 12 months. In terms of economics, pure coconut oil has higher selling price compared to traditional coconut oil by heating coconut milk or precipitating coconut milk in a long time so as to produce rancid odor and poor quality due to high water content and free fatty acid. The purpose of this study was to obtain pure coconut oil with lower water content and free fatty acids according to SNI. There are 3 methods of making pure coconut oil that is method of fermentation, induction and heating, while the method was used in this research is the method of heating. Heating method which is tested by gradual heating or once heating, for gradual warming done preheat with temperature 60-110 ° C with 9 hours of drying and heating time with 60 ° C temperature variations (sample A), 70 ° C (sample B), 80 ° (sample C) and 5 hours, 4 hour and 3 hour drying time. The results showed that drying air temperature had an effect on product quality and length of drying time. The results of the calculation and testing showed the most effective treatment that is on the sample A with water content and free fatty acid content of the lowest reached 0.08% and 0.10%.

Exocrine Secretions of Wheel Bugs (Heteroptera: Reduviidae: Arilus spp.): Clarification and Chemistry

Wheel bugs (Heteroptera: Reduviidae: Harpactorinae: Arilus) are general predators, the females of which have reddish-orange subrectal glands (SGs) that are eversible like the osmeteria in some caterpillars. The rancid odor of Arilus and other reduviids actually comes from Brindley's glands, which in the North (A. cristatus) and South (A. carinatus) American wheel bugs studied emit similar blends of 2-methylpropanoic, butanoic, 3-methylbutanoic, and 2-methylbutanoic acids. The Arilus SG secretions studied here are absolutely species specific. The volatile SG components of A. carinatus include (E)-2-octenal, (E)-2-nonenal, (E)-2-decenal, (E,E)-2,4-nonadienal, (E)-2-undecenal, hexanoic acid, 4-oxo-nonanal, (E,E)-2,4-decadienal, (E,Z)-2,4- or (Z,E)-2,4-decadienal, and 4-oxo-(E)-2-nonenal; whereas in A. cristatus the SG secretion contains β-pinene, limonene, terpinolene, terpinen-4-ol, thymol methyl ether, α-terpineol, bornyl acetate, methyl eugenol, β-caryophyllene, caryophyllene oxide, and farnesol. Arilus spp. SG secretions may be sex pheromones, but verification of this hypothesis requires further testing.