scholarly journals Blood-Glucose Levels of Rats Given High-Fat Diets after Administration of Neem Leaf Ethanolic Extract

2021 ◽  
Vol 13 (2) ◽  
pp. 142-148
Author(s):  
Sri Isdadiyanto ◽  
Siti Muflichatun Mardiati ◽  
Agung Janika Sitasiwi
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Feed Consumption ◽  
Antidiabetic Activity ◽  
Control Group ◽  
High Fat ◽  
Significance Level ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

Azadirachta indica A. Juss (Neem) is a medicinal plant that contains antioxidants, antihyperlipidemia, and has antidiabetic activity. This research aimed to examine the effect of neem leaf ethanolic extract on blood glucose levels of white rats given high-fat rations. The male white rats (Rattus norvegicus L.) with two months of age were used. The treatment of test animals consisted of six treatment groups and four replications, namely: the control group (P0); given high fat rations + duck egg yolk 2.5 ml/200gBW (P1); given high-fat rations + 8 mg/200gBW simvastatin (P2); and P1+ the dose of neem leaf ethanolic extract of 75; 100; and 125 mg/200gBW (P3; P4; and P5). Blood-glucose levels were analyzed by colorimetric enzymatic method using Roche Diagnostics Cobas C reagents kits. Blood-glucose level was determined using Roche / Hitachi cobas c systems automatically. Homogeneous and normally distributed data of blood-glucose levels, body weight and feed consumption were analyzed statistically using ANOVA followed by Duncan's test with a significance level of 5% using SPSS 16.0 software. The results indicated that giving the neem leaf ethanolic extract of could reduce the blood glucose levels. In this study, it was concluded that the neem leaf ethanolic extract of could potentially be used as an alternative to cure diabetes mellitus.

scholarly journals UJI AKTIVITAS ANTIHIPERGLIKEMIA EKSTRAK ETANOL 70% Echinometra mathaei PADA TIKUS PUTIH JANTAN GALUR WISTAR YANG DIINDUKSI ALOKSAN

2021 ◽  
Vol 2 (1) ◽  
pp. 29
Author(s):  
Angelica Kresnamurti ◽  
Nana Angelica ◽  
Farizah Izazi
Keyword(s):  
Blood Glucose ◽  
Sea Urchin ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Standard Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Echinometra Mathaei

Abstract : Sea urchin (Echinometra mathaei) contains a compound of Echinochrome A, which is a polyhydroxy naphthoquinone that is known to be potential antioxidant. The antioxidant effect correlated with the antidiabetic activity that associated with oxidative stress conditions. The aim of this study was to determine the effect of 70% Ethanol Extract of sea urchin (Echinometra mathaei) on blood glucose levels in alloxan induced rats. This research was an experimental study using 30 male white rats, divided into 6 groups, i.e the negative control group (normal rat were only given CMC Na 0,5%), Positive control group (hyperglycemic rats were only treated with CMC Na 0,5%), metformin group as a refference standard group (hyperglycemic rats that treated with metformin) and the treatment group (hyperglycemic rats were treated with ethanol extract 70% of Echinometra mathaei dose of 100 mg/kgBW, 200 mg/kgBW and 400 mg/kgBW). Alloxan monohidrat 120 mg/KgBW was used as diabetogen agent. The parameters examined in this study were blood glucose levels of rats after 7 days of treatment. The observational data were analyzed using the One Way ANOVA (P < 0.05) and continued with LSD. The results of this study showed that all doses of ethanol extract significantly reduced blood glucose levels in rats compared to the control group. The most effective dose in reducing blood glucose levels and compared to metformin was 400 mg / kgBW. Key word: alloxan induced, Echinometra mathaei, ethanol 70%, hiperglycemia, marine product.

scholarly journals UJI ANTIDIABETIK EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 160
Author(s):  
Adinda Fransisca Pongoh ◽  
Edwin De Queljoe ◽  
Henki Rotinsulu
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Carica Papaya ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Control Group ◽  
Homogeneity Test ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

ABSTRACT This study aims to determine the antidiabetic activity of papaya flower ethanol extract (Carica papaya L.) against male white rats (Rattus norvegicus) induced by alloxan. This research is experimental. Fifteen rats were divided into 5 treatment groups, each group consisted of 3 rats. The first group was the negative control group given Aquades, the second group was positive control given Glibenklamid, the three groups were 200 mg dose variation groups, four groups were 400 mg dose variations, and the five groups were 800 mg dose variations. Previously, rats were examined fasting blood glucose levels, then mice were induced by an alloxan dose of 120 mg / kgBW intraperitoneally. On the 3rd day blood glucose levels were examined and then treated according to groups for 7 days, measurement of blood glucose levels after the treatment was carried out once every 3 days namely day 3, day 7, and day 10. Data obtained were then analyzed statistically using SPSS , including normality test (Shapiro-Wilk), homogeneity test (Levene), ANOVA test (One way). The results of this study indicate that Papaya Flower (Carica papaya L.) Ethanol Extract can provide the best blood glucose level reduction effect at a dose of 800 mg. Keywords : Antidiabetic, Papaya Flower Extract (Carica papaya L.), Diabettes Mellitus, Male White Rat (Rattus norvegicus), Aloxan.  ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas Antidiabetik Ekstrak Etanol Bunga Pepaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Penelitian ini bersifat eksperimental. Sebanyak 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif yang diberikan Aquades, Kelompok kedua Kontrol Positif yang diberikan Glibenklamid, kelompok tiga kelompok variasi dosis 200 mg, kelompok empat kelompok variasi dosis 400 mg, dan kelompok lima kelompok variasi dosis 800 mg. Sebelumnya tikus dilakukan pemeriksaan kadar glukosa darah puasa, selanjutnya tikus diinduksi Aloksan dosis 120 mg/kgBB secara intraperitoneal. Pada hari ke 3 diperiksa kadar Glukosa darah kemudian diberikan perlakuan sesuai kelompok selama 7 hari, pengukuran kadar glukosa darah setelah perlakuan dilakukan 3 hari sekali yaitu hari ke 3, hari ke 7, dan hari ke 10. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS, meliputi uji normalitas (Shapiro-Wilk), uji homogenitas (Levene), uji ANOVA (One way). Hasil penelitian ini menunjukkan bahwa Ekstrak Etanol Bunga Pepaya (Carica papaya L.)  dapat memberikan efek penurunan kadar glukosa darah yang paling baik yaitu pada dosis 800 mg. Kata kunci : Antidiabetik, Ekstrak Bunga pepaya (Carica papaya L.), Diabettes Mellitus, TikusPutih Jantan (Rattus norvegicus), Aloksan.

The Effect of Ethanol Extract of Guava Leaf on Hypercholesterolemia-Diabetes of White Rats Male Induced by High Fat Feed and Streptozotocin

2021 ◽  
Vol 7 (2) ◽  
pp. 177-187
Author(s):  
Joni Tandi ◽  
Franciska Diana Pratiwi ◽  
Surisna Surisna ◽  
Niluh Puspita Dewi ◽  
Magfirah Magfirah
Keyword(s):  
Blood Glucose ◽  
Effective Dose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Group I ◽  
Guava Leaf

This study aims to determine the effect of guava leaf extract (Psidiium guajava L) on hypercholesterolemia-diabetes and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves had an effect on reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effectively reduce cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

scholarly journals Taxithelium napalense acts against free radicals and diabetes mellitus

2017 ◽  
Vol 12 (2) ◽  
pp. 16 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Kishore Naidu Killari ◽  
Vedula Girija Sastry ◽  
Alekhya Ketha
Keyword(s):  
Free Radicals ◽  
Blood Glucose ◽  
Video Clip ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Radical Scavenging Properties ◽  
Histopathological Studies

<p class="Abstract">In this work, <em>Taxithelium nepalense</em> ethanolic extract and its fractions were evaluated for the antidiabetic activity in rat based on phytochemical and free radical scavenging properties. The ethanolic extract, fraction IV and V significantly attenuated the blood glucose levels at 600, 200 and 200 mg/kg with 50.0, 33.5 and 42.0% inhibition respectively. The histopathological studies were manifesting the recuperation of damaged cells in liver and pancreas tissues. The outcomes of the present work affirm that the <em>T. nepalense</em> has a potency to plummet the overproduction of free radicals and blood glucose levels in the diabetic-induced rat.</p><p><strong>Video Clip of Methodology</strong>:</p><p>1 min 50 sec   <a href="https://youtube.com/v/GUZp4QY9kCs">Full Screen</a>   <a href="https://youtube.com/watch?v=GUZp4QY9kCs">Alternate</a></p>

scholarly journals Study on optimization and stability of a single dose streptozotocin-induced diabetic modeling in rats

2020 ◽  
Author(s):  
Yao Zhang ◽  
jiao Zhang ◽  
Ming Hong ◽  
Jingyi Huang ◽  
Rui Wang ◽  
...  
Keyword(s):  
Blood Glucose ◽  
High Fat Diet ◽  
Female Rats ◽  
Male Rats ◽  
Control Group ◽  
High Fat ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Sd Rats ◽  
Male And Female Rats

Abstract BackgroundOptimization of experimental conditions in streptozotocin induced diabetic model in Sprague Dawley (SD) rats to evaluate the stability of the model.MethodsMale and female SD rats were randomly divided into control group, STZ 45 group (STZ: 45 mg / kg), STZ 65 group (STZ: 65 mg / kg), STZ 85 group (STZ: 85 mg / kg), high fat diet with STZ 45 group (STZ: 45 mg / kg), high fat diet with STZ 65 group (STZ: 65 mg / kg), high fat diet with STZ 85 group (STZ: 85 mg / kg). N = 6 in each group. The changes of body weight and blood glucose were observed dynamically.ResultsThere was no significant difference in blood glucose or body weight between the STZ 45 group and the control group in both male and female rats, whether or not they were on a high-fat diet. However, there were significant differences in blood glucose between the high-dose STZ group and the control group in both male and female rats, regardless of whether the rats were on a high-fat diet or not (P < 0.05 or P < 0.01). Compared with the control group, there were significant differences in blood glucose levels (P < 0.05 or P < 0.01) and higher blood glucose levels in the male rats fed with the normal diet than that in those fed with the high-fat diet.ConclusionsIn this study, male rats fed with ordinary feed and injected STZ dose of 65 mg / kg were the most stable and ideal diabetic rat.

scholarly journals PENGARUH EKSTRAK BAWANG HITAM TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 49
Author(s):  
Pipit Festi W
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Mus Musculus ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Significance Level ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

 Administration of oral hypoglycemic drugs and insulin is one of the most common pharmacological treatments given to people with diabetes mellitus. This treatment is carried out in the long term so that it causes side effects and is carried out continuously so that it will affect the financial condition of the diabetes mellitus patient. The purpose of this study was to determine the effect of black garlic extract on decreasing blood glucose levels in mice (mus musculus). This study uses the design of True Experimental design (pretest-posttest with control group design). The population is mice in Pusvetma Surabaya. Sampling method uses Non Probability Sampling Purposive Sampling type. Samples taken were 30 mice consisting of 15 intervention groups and 15 control groups. Data was collected using observation sheets, then analyzed using Independent sample t test and Dependent sample t test with a significance level of 5 0.05. The results showed: Using the test Independent sample T test obtained a significance value ρ = 0,000  It can be concluded that there is an effect of black onion extract to reduce blood glucose levels in mice. In the paired t-test test in the treatment group showed ρ = 0,000  that there were differences in blood glucose levels before being given extracts of garlic and after being given intervention. It is expected that black garlic can be used as an alternative medicine to reduce blood glucose levels.Keyword : Ekstrak of black garlic, Blood sugar levels, mus musculus

scholarly journals Decreased Blood Glucose and Histopathology Improvement in Hyperbaric Hyperoxic Conditions Alloxan-induced rats(Laboratory Experimental Study)

2019 ◽  
Vol 12 (4) ◽  
pp. 54-62
Author(s):  
Retno Budiarti
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Hyperbaric Oxygen ◽  
Control Group ◽  
Glucose Levels ◽  
Oxygen Exposure ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Significant Difference ◽  
Hyperbaric Oxygen Exposure

Background: Diabetes mellitus is still a problem in Indonesia, related to the number of organs involved. This disease has relatively high morbidity and mortality rates. Adjuvant therapy is needed considering the long-term therapy that must be consumed by patients. Objective: To explain the effect of hyperbaric oxygen in reducing blood glucose levels, and repairing histopathological damage to the pancreas and liver. Method : this study was an experimental laboratory study using whistar strain rats (Rattus Norvegicus) which were given a normal diet then induced alloxan to create a hyperglycemia condition. After that, 12 rats from the treatment group were given 3 x 30’ hyperbaric oxygen exposure for 6 days. Blood glucose levels, histopathological changes in the pancreas and liver was measured between the treatment group and the control group were not given hyperbaric oxygen exposure. Results: There was a significant difference (α< 0,05) in decreasing blood glucose and repairing histopathological damage in pancreatic and liver tissue between treated group and control group. Conclusion: hyperbaric oxygen treatment as much as 3 x 30'for days at 2.4 ATA O2 100% reduce blood glucose levels and repair histopathological damage to pancreatic tissue and liver of alloxan- induced white rats.

scholarly journals PRELIMINARY PHYTOCHEMISTRY AND ANTIDIABETIC ACTIVITY OF PORTULACA GRANDIFLORA HOOK PLANT EXTRACT ON STREPTOZOTOCIN-INDUCED DIABETES IN RATS

2019 ◽  
pp. 87-90
Author(s):  
DEVI M ◽  
KOMAL S ◽  
LOGESHWARI B
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Portulaca Grandiflora ◽  
Streptozotocin Induced Diabetes

Objective: The present study was aimed to evaluate the antidiabetic activity of ethanolic extract of the whole aerial plant of Portulaca grandiflora Hook on streptozotocin (STZ)-induced diabetic rats. Methods: Experimental diabetes was induced by a single dose of intraperitoneal injection of STZ (150 mg/kg). Adult male Wister albino rats were divided into five groups; normal control, diabetic control, diabetic glibenclamide (5 mg/kg), diabetic P. grandiflora H. extract (200 mg/kg), and diabetic P. grandiflora H. extract (400 mg/kg) for 21 days and analyzed for body weight (BW) and blood glucose. Results: The STZ-treated diabetic control rats showed a significant increase in blood glucose with a concomitant decrease in BW. Oral administration of P. grandiflora H. extract (200 and 400 mg/kg) and glibenclamide (5 mg/kg) for 21 days showed a significant reduction in blood glucose levels and elevation in the bodyweight studies as compared to control and glibenclamide-treated rats. Conclusion: The results of the present study showed that a potent antidiabetic activity was present in the aerial part of plant P. grandiflora H. extract.

scholarly journals ANTIDIABETIC ACTIVITY TEST USING AMLA FRUIT (PHYLLANTHUS EMBLICA L) EXTRACT IN ALLOXAN-INDUCED BALB/C MICE

2019 ◽  
Vol 3 (2) ◽  
pp. 53
Author(s):  
Putu Lakustini Cahyaningrum ◽  
Sang Ayu Made Yuliari ◽  
Ida Bagus Putra Suta
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Positive Control ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Phyllanthus Emblica ◽  
Fruit Extract ◽  
Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: The content of secondary metabolites in amla (phyllanthus emblica L.) such as flavonoids and phenols have the potential as an antioxidant. One of the benefits of antioxidants is to prevent degenerative diseases, such as diabetes mellitus. Purpose: This research to determine activity of amla fruit extract in reducing blood glucose levels at balb/c mice induced with alloxan. Methods. This research method uses a pre and posttest-controlled group design with 35 balb/c mice divided into five treatments groups. In the positive control group and the treatment group alloxan was injected for 14 days. Results: Amla (Phyllanthus emblica L.) fruit extract at a dose of 40 mg / 20 g BW was equivalent to positive control of glibenclamide dose 3 mg / 20 g BW compared to the treatment group at a dose of 10 mg / 20 g BW and 20 mg /20 g BW. Conclusion: From this research, it was obtained that the best dose of Amla fruit (phylanthus emblica L) extract applied to blood glucose was 40 mg/20mg BW with a decrease percentage of 56,93% with an effective dose value (ED50) 50% of 34.00 mg / 20 g BW.

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