scholarly journals Anti-hyperlipidemic effect of Vitex doniana in poloxamer induced hyperlipidemia

2018 ◽  
Vol 3 (4) ◽  
pp. 168-173 ◽  
Author(s):  
Victor Duniya Sheneni ◽  
Victor Akagwu Odiba ◽  
Ameh Omede ◽  
Favour Moses Idih
Keyword(s):  
Normal Control ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Stem Bark ◽  
Normal Control Group ◽  
Poloxamer 407 ◽  
Control Group ◽  
Root Bark ◽  
Risk Predictor ◽  
Vitex Doniana

To determine the effect of Vitex doniana (leaves stem and root bark) ethanol extracts on lipid profiles of Poloxamer 407 (P-407) induced hyperlipidemic and normal rats. Fifty four mixed sex rats weighing 100-200g were divided into nine groups comprising six animals per group: group given feed and water only, group induced by an intra-peritoneal injection of P-407 every 48hours without treatment, groups induced and treated with atorvastatin, leaves, stem bark, root bark extracts and groups of normal rats treated with leaves, stem bark and root bark extracts without induction. In all the groups, P-407, atorvastatin, leaves, stem bark and root bark extracts were administered at a dose of 1000mg/kg, 20mg/kg, 100mg/kg, 100mg/kg and 30mg/kg body weight respectively. At the end of the 21day, the animals were sacrificed and blood sample were collected for determination of serum levels of: Total cholesterol (TC), Triacylglycerides (TAG), High-density lipoprotein cholesterol (HDL-c) and Low-density lipoprotein cholesterol (LDL-c). The studies showed that all induced treated groups significantly (P<0.05) lower serum levels of TC, TAG, LDL-c and significantly (P<0.05) increased HDL-c when compared to the P407 induced hyperlipidemic control. The normal treated groups showed no significant (P>0.05) difference in the serum levels of TC, TAG, LDL and HDL when compared to the normal control group. Calculation of atherogenic risk predictor indices of the induced treated groups showed that all the extracts significantly (P<0.05) lowered the LDL-c/HDL-c, log (TAG/HDL-c) and significantly (P<0.05) increased HDL-c/TC ratio when compared to the P407 induced hyperlipidemic control group. The atherogenic risk predictor indices of normal treated groups showed no significant difference (P>0.05) in LDL-c/HDL-c, Log (TAG/HDL-c) and HDL-c/TC ratio when compared to the normal control group. The study demonstrates the phytotherapeutic effect of Vitex doniana (leaves, stem and root bark) ethanol extract in poloxamer 407 induced hyperlipidemia.

Experimental Observation on Antiatheroscloresis Ability of Total Flavonoids from Jumi in Hyperlipidemia Rats

2011 ◽  
Vol 140 ◽  
pp. 137-141
Author(s):  
Guang Tao Xu ◽  
Xiao Wei Wu ◽  
Xin Mei Zhou ◽  
Shi Piao Chen ◽  
Shen Rong Shen
Keyword(s):  
Dose Group ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Normal Control Group ◽  
Atherogenic Index ◽  
Control Group ◽  
Total Flavonoids ◽  
Low Doses ◽  
Control Groups

In this study, we observed antiatheroscloresis ability of food-roune hyperlipidemia by total falconoid from Jumi in rats. We used hyperlipidemia model of rats established, intervened by total falconoid from Jumi. Rats were divided into normal control group, hyperlipidemia control group, higher dose group and lower dose group to observe the level of total cholesterol (TC), trilaurate glycerin (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and atherogenic index (AI), and take rat liver for regular pathological dyeing observation. The results shown that the total falconoid from Jumi could reduce the serum levels of TC, TG, LDL and AI significantly, and increase HDL/TC in compared with hyperlipidemia control groups. It had significantly curative to effect hepatic adipose infiltration in rats, especially in low doses of flavonoid. Total flavonoids from Jumi can regulate disturbance of lipid metabolism, hepatic adipose infiltration and arteriosclerosis.

scholarly journals Methanol extract of Aruncus dioicus exerts antidiabetic effect via PCSK9/LDLR pathway

2021 ◽  
Vol 18 (3) ◽  
pp. 571-577
Author(s):  
Li Gong ◽  
Qing Yang ◽  
Yinluan Huang ◽  
Shaoyan Xie ◽  
Chao Zeng ◽  
...  
Keyword(s):  
Normal Control ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Normal Control Group ◽  
Control Group ◽  
Antidiabetic Effect ◽  
Treatment Groups ◽  
Diabetic Control Group

Purpose: To investigate the antidiabetic effect of methanol extract of Aruncus dioicus, and the underlying mechanism(s). Methods: Twenty-four adult female albino mice were randomly assigned to four groups of six mice each: normal control group, diabetic control group and two treatment groups. With the exception of normal control group, the diabetic control and treatment groups consisted of leptin receptor-deficient (db/db) type 2 diabetic mice. The diabetic control group was not treated, while the treatment groups received 200 or 400 mg/kg extract/day orally for 4 weeks. The effect of the extract on fasting blood glucose (FBG), proprotein convertase subtilisin/kexin type 9 (PCSK9), glycogen and lipid profiles were determined. The expressions of PCSK9, low-density lipoprotein receptor (LDL-R) and glucokinase (GCK) were determined in liver tissues using western blotting and real-time quantitative polymerase chain reaction (qRT-PCR). Results: Fasting blood glucose (FBG) was significantly and dose-dependently reduced in the treatment groups, relative to diabetic control group at different time-points (p < 0.05). Total cholesterol (TC), triacylglycerol (TG), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were significantly higher in the diabetic control group than in normal control group (p < 0.05). However, treatment with methanol extract of A. dioicus significantly and dose-dependently reversed the changes in the levels of these parameters (p < 0.05). The expressions of LDLR and GCK were significantly down-regulated in diabetic control group, when compared with normal control group, but their expressions were significantly dose-dependently upregulated in the treatment groups (p < 0.05). Treatment with the extract significantly and dose-dependently down-regulated PCSK9 expression (p < 0.05). Liver injury characterized by large distended lipid droplets and fat accumulation was seen in diabetic mice, but treatment with methanol extract of A. dioicus significantly reversed the histopathological changes induced by DM. Conclusion: These results indicate that the antidiabetic effect of methanol extract of A. dioicus is exerted via a mechanism involving PCSK9/LDLR pathway.

scholarly journals Effects of ethanolic extracts of Datura metel on blood lipid profile of male albino rats

2016 ◽  
Vol 2 (10) ◽  
pp. 248 ◽  
Author(s):  
Kayode A. Arowora ◽  
Chinedu Imo ◽  
Chukwuma S. Ezeonu ◽  
Zuhairah I. Muhammad
Keyword(s):  
Lipid Profile ◽  
Normal Control ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Normal Control Group ◽  
Control Group ◽  
Albino Rats ◽  
Blood Lipid Profile ◽  
Datura Metel ◽  
Ethanolic Extracts

<p class="abstract"><strong>Background:</strong> This study was carried out to investigate the effects of <em>Datura metel (</em>leaf, seed and fruit<em>)</em> on blood lipid profile of male albino rats.</p><p class="abstract"><strong>Methods:</strong> Thirty-five albino rats (8 weeks old) weighing between 66g and 84g were purchased and randomly allotted into 7 groups. The normal control (group 1) received normal saline, while groups 2 to 7 received extracts of <em>Datura metel</em> at low (300 mg/kg body weight) and high doses (600 mg/kg body weight). The extracts were administered orally for seven consecutive days, while the animals were sacrificed on the 8th day; blood samples were collected, allowed to stand for fifteen minutes and then centrifuged.  </p><p class="abstract"><strong>Results:</strong> There was a general decreasing trend in the mean values of low density lipoprotein levels across  the groups, however, group 7 (with lowest value) was found to be significantly lower (p &lt;0.05) than other groups when compared with the normal control. Also, there was a general decreasing trend in the values of high density lipoprotein compared with normal control group, however lowest value was recorded to be 1.35±0.06 mmol/l for group 5. There were no significant differences (p &gt;0.05) in triglyceride levels across the groups, while total cholesterol in groups 5 and 6 had significant differences with values of 2.05±0.06 mmol/l and 2.13±0.10 mmol/l respectively when compared with the normal control.</p><p><strong>Conclusions:</strong> This study suggests that ethanolic extracts of<em> Datura metel </em>have active ingredients that are capable of improving blood lipid profile and this might be useful in the management of cardiovascular diseases. </p>

scholarly journals Clinical significance of serum AMH and INHB in girls with precocious puberty

2021 ◽  
Author(s):  
Qingxu Liu ◽  
Xiaoqin Yin ◽  
Pin Li
Keyword(s):  
Clinical Significance ◽  
Normal Control ◽  
Precocious Puberty ◽  
Roc Analysis ◽  
Clinical Manifestations ◽  
Tanner Stage ◽  
Serum Levels ◽  
Normal Control Group ◽  
Control Group ◽  
Significant Difference

Abstract Background: Precocious puberty is the second sexual characteristic of girls before 8 years old. The diagnosis of central precocious puberty (CPP) needs to evaluate ovarian function, and ultrasound examination is an auxiliary means for the evaluation of ovary at present. The serum levels of AMH and INHB might be markers of evaluation of ovary according the former research. We investigated the clinical features, serum sex hormones, serum levels of AMH and INHB in 184 girls with precocious puberty, which provided deeper insight into the clinical significance of AMH and INHB in female precocious puberty. Methods: We evaluated 184 girls with precocious puberty at the Department of Endocrinology of Shanghai Children's Hospital from 2017 to 2021, which was consisted of PT, Tanner stage 2 CPP and Tanner stage 3 CPP. We analysed clinical data from the patients including clinical manifestations, AMH, INHB and other hormone levels, and we analysed AMH and INHB in normal control group either.Results: The PT group (P = 0.031) and Tanner stage 2 CPP group (P = 0.006) exhibited significantly higher AMH level than that in normal control group. AMH level showed no significant difference among PT group, Tanner stage 2 CPP group and Tanner stage 3 CPP group. The Tanner stage 2 CPP group exhibited significantly higher level of INHB than that in Tanner stage 3 CPP group (P = 0.013) and normal control group (P = 0.007). AMH and INHB were positively correlated in the four groups, especially in the PT group (r = 0.694**, P < 0.01). AMH and basal LH were positively correlated in PT group (r = 0.296*, P < 0.01). AMH or INHB showed no correlation with chronical age, bone age, uterine volume, ovarian volume, BMI, E2, SHBG, peak LH, basal FSH, peak FSH, IGF-1 or IGF-BP3. The ROC analysis showed that the AUC of AMH or INHB was relatively low. Conclusions: In this research, the serum AMH and INHB in 184 girls with precocious puberty were analysed. The PT group and Tanner stage 2 CPP group exhibited significantly higher AMH level than that in normal control group. The Tanner stage 2 CPP group exhibited significantly higher level of INHB than that in Tanner stage 3 CPP group and normal control group. AMH and INHB were positively correlated in the four groups, especially in the PT group. ROC analysis showed that the diagnostic performance of PT or CPP using AMH or INHB was weak.

The Serum Complement System in Children on Continuous Ambulatory Peritoneal Dialysis

1993 ◽  
Vol 13 (3) ◽  
pp. 214-218 ◽  
Author(s):  
Roel E. Reddingius ◽  
Cornelis H. Schröder ◽  
Mohamed R. Daha ◽  
Leo A.H. Monnens
Keyword(s):  
Renal Failure ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Normal Control ◽  
Serum Levels ◽  
Normal Control Group ◽  
University Hospital ◽  
Control Group ◽  
Significant Difference ◽  
Normal Controls

Objective During continuous ambulatory peritoneal dialysis (CAPD), the loss of complement factors via the dialysate may cause complement deficiencies. This hypothesis was tested in a group of children treated with CAPD. Design Classical (CH50) and alternative (AP50) complement activity and serum levels of factors C1 q, C3, C4, C3d, B, D, and P in CAPD patients were compared to normal controls and to children with preterminal renal failure. Setting Patients were seen in a university hospital; normal controls were seen in an outpatient clinic of a general hospital. Patients A group of 22 children on CAPD was compared to a normal control group of 44 children and to a group of 12 children with preterminal renal failure with a creatinine clearance below 25 mL/min/1.73 m2. Results CH50, AP50, C3, and B were not significantly different from the control group in both the CAPD and preterminal groups. Factors C1q (p=0.01) and C4, C3d, D, and P (p<0.001) were higher in the CAPD group in comparison to the normal control group. The factors D (p<0.001) and P (p=0.02) were also elevated in the preterminal group. For the measured factors there was no significant difference between the CAPD group and the preterminal group. Conclusions There is no deficiency of complement in children treated with CAPD. High levels of C3d and D can be explained by the reduction of their elimination by the kidney. The increased levels of the other factors are presumably due to increased synthesis in renal failure. This does not seem to be caused by CAPD.

scholarly journals Study on potential differentially expressed genes in stroke by bioinformatics analysis

2021 ◽  
Author(s):  
Xitong Yang ◽  
Pengyu Wang ◽  
Shanquan Yan ◽  
Guangming Wang
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Normal Control ◽  
Enrichment Analysis ◽  
Normal Control Group ◽  
Control Group ◽  
Ppi Network ◽  
Hub Genes ◽  
Pathway Enrichment ◽  
Key Genes

AbstractStroke is a sudden cerebrovascular circulatory disorder with high morbidity, disability, mortality, and recurrence rate, but its pathogenesis and key genes are still unclear. In this study, bioinformatics was used to deeply analyze the pathogenesis of stroke and related key genes, so as to study the potential pathogenesis of stroke and provide guidance for clinical treatment. Gene Expression profiles of GSE58294 and GSE16561 were obtained from Gene Expression Omnibus (GEO), the differentially expressed genes (DEGs) were identified between IS and normal control group. The different expression genes (DEGs) between IS and normal control group were screened with the GEO2R online tool. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the DEGs were performed. Using the Database for Annotation, Visualization and Integrated Discovery (DAVID) and gene set enrichment analysis (GSEA), the function and pathway enrichment analysis of DEGS were performed. Then, a protein–protein interaction (PPI) network was constructed via the Search Tool for the Retrieval of Interacting Genes (STRING) database. Cytoscape with CytoHubba were used to identify the hub genes. Finally, NetworkAnalyst was used to construct the targeted microRNAs (miRNAs) of the hub genes. A total of 85 DEGs were screened out in this study, including 65 upward genes and 20 downward genes. In addition, 3 KEGG pathways, cytokine − cytokine receptor interaction, hematopoietic cell lineage, B cell receptor signaling pathway, were significantly enriched using a database for labeling, visualization, and synthetic discovery. In combination with the results of the PPI network and CytoHubba, 10 hub genes including CEACAM8, CD19, MMP9, ARG1, CKAP4, CCR7, MGAM, CD79A, CD79B, and CLEC4D were selected. Combined with DEG-miRNAs visualization, 5 miRNAs, including hsa-mir-146a-5p, hsa-mir-7-5p, hsa-mir-335-5p, and hsa-mir-27a- 3p, were predicted as possibly the key miRNAs. Our findings will contribute to identification of potential biomarkers and novel strategies for the treatment of ischemic stroke, and provide a new strategy for clinical therapy.

Effect of GDM on the Expression of MT1-MMP and u-PA in Glioma Cells

2014 ◽  
Vol 1033-1034 ◽  
pp. 220-223
Author(s):  
Xue Mei Han ◽  
Li Bo Wang ◽  
Ni Ni Li ◽  
Song Yan Liu
Keyword(s):  
Normal Control ◽  
Glioma Cell ◽  
Fetal Bovine Serum ◽  
Glioma Cells ◽  
Normal Control Group ◽  
Control Group ◽  
Rt Pcr ◽  
Glioma Cell Lines ◽  
Fetal Bovine ◽  
Identify Gene Expression

To examine the effect of GDM on the expression of MT1-MMP and u-PA genes in glioma cells. Glioma cell lines U251 and U87 were cultured in DMEM medium supplemented with 10% fetal bovine serum. RT-PCR was used to identify gene expression level. The level of u-PA mRNA was up-regulated significantly in the HGF group compared with the normal control group (P<0.05). The expression of MT1-MMP and u-PA was significantly lower in the GDM group than in the normal control and HGF groups (P<0.05). The expression of u-PA in the HGF+GDM group was down-regulated significantly compared with the normal control and HGF groups (P<0.05).GDM can inhibit expression of both MT1-MMP and u-PA in glioma cells.

Identification of Hub Genes Associated with Development of Lung Adenocarcinoma by Integrated Bioinformatics Analysis

2021 ◽  
Author(s):  
Jinglei Li ◽  
Wei Hou
Keyword(s):  
Gene Expression ◽  
Survival Analysis ◽  
Network Analysis ◽  
Lung Adenocarcinoma ◽  
Normal Control ◽  
Gene Expression Analysis ◽  
Normal Control Group ◽  
Control Group ◽  
Differential Gene Expression Analysis ◽  
Differential Gene

Abstract Purpose: Lung adenocarcinoma (LUAD) has high heterogeneity and poor prognosis, posing a major challenge to human health worldwide. Therefore, it is necessary to improve our understanding of the molecular mechanism of LUAD in order to be able to better predict its prognosis and develop new therapeutic strategies for target genes.Methods: The Cancer Genome Atlas and Gene Expression Omnibus, were selected to comprehensively analyze and explore the differences between LUAD tumors and adjacent normal tissues. Critical gene information was obtained through weighted gene co-expression network analysis (WGCNA), differential gene expression analysis, and survival analysis.Results: Using WGCNA and differential gene expression analysis, 29 differentially expressed genes were screened. The functional annotation analysis showed these genes to be mainly concentrated in heart trabecula formation, regulation of inflammatory response, collagen-containing extracellular matrix, and metalloendopeptidase inhibitor activity. Also, in the protein–protein interaction network analysis, 10 central genes were identified using Cytoscape's CytoHubba plug-in. The expression of CDH5, TEK, TIMP3, EDNRB, EPAS1, MYL9, SPARCL1, KLF4, and TGFBR3 in LUAD tissue was found to be lower than that in the normal control group, while the expression of MMP1 in LUAD tissue was higher than that in the normal control group. According to survival analysis, the low expression of MYL9 and SPARCL1 was correlated with poor overall survival in patients with LUAD. Finally, through the verification of the Oncomine database, it was found that the expression levels of MYL9 and SPARCL1 were consistent with the mRNA levels in LUAD samples, and both were downregulated.Conclusion: Two survival-related genes, MYL9 and SPARCL1, were determined to be highly correlated with the development of LUAD. Both may play an essential role in the development LUAD and may be potential biomarkers for its diagnosis and treatment in the future.

scholarly journals Hepatotoxicity and Histological Evaluation of Aqueous and Methanolic Leaf Extracts of Thaumatococcus Daniellii and Alchornea Cordifolia in Wistar Rat Models

2019 ◽  
Vol 1 (2) ◽  
pp. p42
Author(s):  
Service @ Ideasspread.org ◽  
Okafor I. J. ◽  
Nweke E. O. ◽  
Ewa O.
Keyword(s):  
Normal Control ◽  
Wistar Rat ◽  
Normal Control Group ◽  
Histological Evaluation ◽  
Control Group ◽  
Significant Elevation ◽  
Leaf Extracts ◽  
Group Iii ◽  
Methanolic Extracts ◽  
Group I

This study was carried out to ascertain the hepatotoxic potential of T.daniellii (T.d) and A. cordifolia (A.c). Investigations were conducted using standard methods. Oral administration of 200mg/kg aqueous leaf extracts of T.daniellii caused a non-significant increase in the activity of ALT (5.43±0.60IU/L), AST (16.93±0.26 IU/L) and ALP (160.70±1.04 IU/L) compared to the values recorded on the normal control (group I) ALT (3.84±0.16 IU/L), AST (14.19±0.52 IU/L) and ALP (157.26±0.64 IU/L). Group III administered with 200mg/kg methanolic leaf extract of T. daniellii manifested a significant elevation in the activity of ALT (13.15±0.89 IU/L), AST (22.84±0.38 IU/L) and ALP (170.40±0.44 IU/L) compared to the normal control. Similarly, groups IV and V which were orally administered with 200mg/kg aqueous and methanolic leaf extracts of A. cordifolia showed significant increase in the activity of ALT (6.32±0.33U/L), AST (17.70±0.030U/L) and ALP (161.13±0.09U/L) and ALT (7.55±0.59U/L), AST (19.35±0.26U/L) and ALP (165.38±0.35U/L) respectively compared to the values recorded on the control (group I). In conclusion, drug development protocols involving T. daniellii leaf should preferably use water as an ideal solvent. On the other hand, the hepatotocity associated with both aqueous and methanolic extracts of A. cordifolia could imply the presence of hepatotoxins in the leaf of the said plant.

scholarly journals Effects of Hamo NK hard capsule on serum lipid profiles in dyslipidemia experimental animals

2021 ◽  
Vol 141 (5) ◽  
pp. 10-18
Author(s):  
Pham Thuy Phuong ◽  
Pham Quoc Binh ◽  
Dinh Thi Hong Minh ◽  
Tran Thi Thu Hien ◽  
Nguyen Trong Thong ◽  
...  
Keyword(s):  
Serum Lipid ◽  
Therapeutic Strategy ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Poloxamer 407 ◽  
Control Group ◽  
Cholesterol Levels ◽  
Daily Dose ◽  
Previously Treated ◽  
Cholesterol Control

Dyslipidemia is a major risk factor for cardiovascular disease. Polyherbal formulation is a traditional therapeutic strategy used to treat dyslipidemia over many years of tradition. The aim of this study was designed to evaluate the effects of Hamo NK hard capsule on endogenous dyslipidemia and exogenous dyslipidemia experimental animal model. In endogenous hyperlipidemia model, mice were previously treated by Hamo NK hard capsule, and intraperitoneally injected by poloxamer - 407 to induce hyperlipidemia. Rats were oral administration of oil - cholesterol mixture and Hamo NK for 4 consecutive weeks (exogenous dyslipidemia). Parameters of serum lipid were determined. Hamo NK ameliorated the elevation of serum total cholesterol, Non - HDL - cholesterol at the daily dose of 1.5g/kg b.w (p < 0.05). Also, there was no signicant difference in increase on high - density lipoprotein cholesterol levels and decrease triglyceride levels between the groups. Hamo NK at two doses of 0.25g/kg b.w and 0.75g/kg b.w significantly reduced serum LDL - C levels compared to the cholesterol control group. Hamo NK hard capsule affected on serum lipid modulations in dyslipidemia models.

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