scholarly journals PERBANDINGAN EFEKTIVITAS TEMEPHOS DENGAN Bacillus thuringiensis var. israelensis TERHADAP MORTALITAS NYAMUK Aedes aegypti DARI TIGA KELURAHAN DI KOTA SAMARINDA

2019 ◽  
Vol 12 (1) ◽  
pp. 46-53
Author(s):  
Sri Nadyar Ekawati ◽  
Nova Hariani ◽  
Sudiastuti Sudiastuti
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Mosquito Larvae ◽  
Recommended Dosage ◽  
New Type ◽  
The Government

AbstrakPengendalian vektor penyakit demam berdarah (DBD) di Indonesia menggunakan larvasida temephos telah berlangsung lebih dari 30 tahun, oleh karena itu, perlu dilakukan penelitian untuk mengetahui efektivitas temephos dan Bacillus thuringiensis var. israelensis yang merupakan larvasida jenis baru dari agen biologi terhadap larva nyamuk Aedes aegypti. Penelitian ini bertujuan untuk mengetahui perbandingan efektivitas temephos dengan B. thuringiensis var. israelensis terhadap mortalitas larva nyamuk Ae. aegypti dari tiga Kecamatan di Samarinda, yaitu Kecamatan Samarinda Utara Kelurahan Gunung Lingai, Samarinda Ulu Kelurahan Air Putih dan Sungai Kunjang Kelurahan Loa Bakung. Metode yang digunakan adalah metode bioassay. Hasil penelitian menunjukan bahwa temephos tidak efektif untuk membunuh larva nyamuk Ae. aegypti dari tiga kelurahan yang diamati, dengan LC50,24jam sebesar 1,88–2,24 ppm dan LC90,24jam sebesar 2,07–3,59 ppm. Sementara itu, B. thuringiensis var. israelensis masih efektif untuk membunuh larva nyamuk Ae. aegypti dari ketiga kelurahan yang diamati, dengan LC50,24jam sebesar 0,93–1,00 mL/50 L air dan LC90,24jam sebesar 1,05–1,11 mL/50 L air. Hal ini berarti penggunaan B. thuringiensis var. israelensis dengan dosis yang dianjurkan pemerintah masih efektif untuk mengendalikan populasi nyamuk Ae. aegypti.AbstractThe control of dengue hemorrhagic fever (DHF) vector in Indonesia using larvicide temephos has been ongoing for more than 30 years. Hence, it is necessary to assess the effectiveness of temephos compared to Bacillus thuringiensis var. israelensis which is a new type of biological larvacide agent against Aedes aegypti mosquito larvae. This study aimed to determine the effectiveness of temephos compared to B. thuringiensis var. israelensis against mortality of Ae. aegypti mosquito larvae sampled from three subdistricts in Samarinda namely Samarinda Utara Subdistrict Gunung Lingai, Samarinda Ulu Subdistrict Air Putih, and Sungai Kunjang Subdistrict Loa Bakung. The method used was bioassay. The results showed that temephos was not effective in killing Ae. aegypti mosquito larvae from the three subdistricts observed, with LC50.24 hours by 1.88–2.24 ppm, and LC90,24 hours by 2.07–3.59 ppm. Meanwhile, B. thuringiensis var. israelensis is still effective in killing Ae. aegypti mosquito larvae from the three subdistricts observed, with LC50.24 hours by 0.93–1.00 mL/50 L of water and LC90,24 hours by 1.05–1.11 mL/50 L of water. Those results mean that the application of B. thuringiensis var. israelensis with the recommended dosage of the government is still effective in controlling the population of Ae. aegypti.

DETECTION OF DENGUE TRANSOVARIAL VIRUS FOR AIDES AEGYPTI MOSQUITOES IN ENDEMIC AREAS OF DENGUE HEMORRHAGIC FEVER

2019 ◽  
Vol 3 (9) ◽  
Author(s):  
Suparmin . ◽  
Arif Widyanto ◽  
Ismi Rajiani
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Positive Sample ◽  
Elisa Method ◽  
Central Java ◽  
Vector Population ◽  
The Government ◽  
Central Java Province

Background: The incidence rate (IR) of Dengue Hemorrhagic Fever per 100,000 population in Central Java has increased dramatically in five years. Banyumas Regency is an endemic area of ​​dengue hemorrhagic fever in Central Java Province. The cause of dengue hemorrhagic fever is four dengue viruses known as DEN-1, DEN-2, DEN-3, and DEN-4. The purpose of this research is to detect transovarial dengue virus in Aedes aegypti mosquito in Banyumas Regency. Methods: The study was conducted by installing ovitrap in Mersi Village, East Purwokerto District, and in Pliken Village, Kembaran District, Banyumas Regency. The study was conducted by installing ovitrap in Mersi Village (9 samples) and Pliken Village (3 samples). The eggs obtained are hatched into larvae in the laboratory. The 3rd and 4th instar larvae were examined for the presence of dengue virus using the Elisa method. Result: The results showed that in Mersi Village, there were two positive samples, and in Pliken Village, there was one positive sample containing dengue virus. The presence of the dengue virus found in the hatched Aedes aegypti larvae (F1) indicates that there has been a transovarial dengue virus from the parent mosquito in its offspring. Conclusion: The study concluded that in Banyumas District, a dengue virus transovarial had occurred in Aedes aegypti mosquitoes. The government and the community should increase efforts to eradicate mosquito nests (dengue hemorrhagic fever) to reduce the vector population of dengue hemorrhagic fever so that cases of dengue hemorrhagic fever can be eliminated.    Keyword: Transovarial dengue virus, Aedes aegypti, Dengue Hemorrhagic Fever

scholarly journals THE EFFECT OF CORN SILK EXTRACT (Zea mays) AS BIOLARVICIDES OF Aedes aegypti MOSQUITO LARVAE IN EFFORTS TO CONTROL SPREAD OF DENGUE HEMORRHAGIC FEVER

2021 ◽  
Vol 16 (1) ◽  
pp. 23
Author(s):  
Rahmad Wahyudi ◽  
Harfina Indriani ◽  
Yogi Abror
Keyword(s):  
Zea Mays ◽  
Side Effects ◽  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Chemical Compounds ◽  
Hemorrhagic Fever ◽  
Mosquito Larvae ◽  
Dengue Vector ◽  
Corn Silk ◽  
Negative Side Effects

Dengue hemorrhagic fever (DHF) is still a serious health problem in the community. DHF is caused by the dengue virus (DENV) and is transmitted by the Aedes aegypti (Ae. aegypti) mosquito, which is the main vector for the virus. In controlling DHF vectors, larvicides are still used. These larvicides contain chemical compounds that have negative side effects affecting      the human body. Therefore, there is a need for natural-based larvacides (biolarvicides). The purpose of this study was to determine the potential of corn silk as a biolarvicide against dengue vector larvae (Aedes aegypti). This study was carried out at the STIKes Ngudia Husada Madura Bangkalan Laboratory and was done by taking corn silk waste samples disposed of as waste from corn farmers and corn traders in Bangkalan Regency. A Thousand samples of Aedes aegypti larvae that had reached instar III were used and divided into five test groups, namely 0 g/L (control), 6.25 g/L, 12.5 g/L, 25 g/L, and 50 g/L . Each group contained 200 larvae. The experiment was repeated 3 times. Data of the number of deaths of Aedes aegypti larvae were collected every 24 hours. The highest dose that could kill Aedes aegypti larvae was at 20 g/L.Keywords     : biolarvicides, corn silk, larvae, Aedes aegypti

scholarly journals Aktivitas Penggunaan Insektisida Komersil oleh Masyarakat di Daerah Endemis Demam Berdarah Dengue di Provinsi Sulawesi Barat

2019 ◽  
Vol 13 (1) ◽  
pp. 59-66
Author(s):  
Malonda Maksud ◽  
Hasrida Mustafa ◽  
Risti Risti ◽  
Nelfita Nelfita ◽  
Murni Murni ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Active Ingredients ◽  
Design Data ◽  
Cross Sectional ◽  
Control Programs ◽  
Endemic Areas ◽  
The Government ◽  
Cross Sectional Design

Abstract Commercial insecticides in Asia are used to control insects including mosquitoes, which are about 28-89% in endemic areas of Dengue Hemorrhagic Fever (DHF). West Sulawesi Province is one of the dengue endemic areas in Indonesia. The study is a part of multicenter research “Map of Aedes aegypti Mosquito susceptibility Against Insecticides in Indonesia in 2015”. This study aims to the describe the use of commercial insecticides in three endemic districts of West Sulawesi using a cross-sectional design. Data obtained through interviews and observations conducted in July-August 2015 at least 100 homes which were randomly selected in each RW/RT. Data were analyzed descriptively to describe the use of insecticides, types of formulations, types of active ingredients, and the duration of use. The study found that 85% of respondents used commercial insecticides. Most of the respondents chose to use mosquitoes coil (83.5%), the most active ingredients found were D-allethrin (43.8%) and Dimefluthrin (30.4). Most respondents (45.8%) have been using commercial insecticides for more than five years and more dominant to apply at night (79.1%). The government needs to control the use of insecticides in the community so that there is no DHF vector resistance to insecticides used in DHF control programs. Abstrak Insektisida komersil di Asia  digunakan untuk mengendalikan serangga termasuk  nyamuk, yang sekitar 28-89% berada di daerah endemis Demam Berdarah Dengue (DBD). Provinsi Sulawesi Barat merupakan salah satu daerah endemis DBD di Indonesia. Studi ini merupakan bagian dari penelitian multicenter “Peta Kerentanan Nyamuk Aedes aegypti di Indonesia Tahun 2015”.  Studi  ini bertujuan untuk mengetahui gambaran penggunaan insektisida komersil di Sulawesi Barat dengan menggunakan desain potong lintang yang dilakukan di tiga kabupaten endemis DBD. Pengambilan data berupa wawancara dan pengamatan yang dilakukan bulan Juli-Agustus 2015 pada minimal 100 rumah yang dipilih secara acak pada masing-masing RW/RT endemis DBD. Data dianalisa secara deskriptif untuk menggambarkan penggunaan insektisida, jenis formulasi, jenis bahan aktif, dan lama penggunaan. Hasil studi menemukan  sebanyak 85% responden menggunakan insektisida komersil.  Sebagian besar responden memilih menggunakan jenis insektisida bakar (koil) (83,5%), bahan aktif yang paling banyak ditemukan adalah D-allethrin(43,8%) dan Dimefluthrin (30,4). Sebagian besar responden (45,8%) sudah lebih dari lima tahun menggunakan insektisida komersil dan lebih dominan mengaplikasikannya pada malam hari (79,1%). Pemerintah perlu mengontrol penggunaan insektisida di masyarakat, agar tidak terjadi resistensi vektor DBD terhadap insektisida program.

scholarly journals UJI EFIKASI EKSTRAK DAUN SEREH (Andropogon nardus) DAN BIJI MAHONI (Swietenia macrophylla) TERHADAP MORTALITAS LARVA NYAMUK Aedes aegypti

2019 ◽  
Vol 5 (2) ◽  
Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Eis Nurhiliya
Keyword(s):  
Data Collection ◽  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Plant Extracts ◽  
Endemic Area ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Seed Extract ◽  
Mosquito Larvae ◽  
Lemon Grass

ABSTRAK              Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak tanaman sereh (Andropogon nardus), biji mahoni (Switenia macrophylla) dan kombinasi keduanya terhadap mortalitas larva nyamuk Aedes aegypti. Larva nyamuk Aedes aegypti untuk sampel adalah larva hasil rearing F2 yang diperoleh dengan ovitrap dari wilayah Kelurahan Kambu yang merupakan wilayah endemis Demam Berdarah Dengue (DBD). Penelitian ini dilakukan di laboratorium Zoologi, FMIPA UHO, Kendari. Ekstrak tanaman sereh (Andropogon nardus) dan ekstrak biji mahoni (Switenia macrophylla) masing-masing dengan konsentrasi 1,5% dan 3,0% dan 4.5%. kombinasi kedua jenis ekstrak tersebut dipaparkan selama 24 jam untuk melihat mortalitas larva nyamuk Aedes aegypti. Pengumpulan data dilakukan dengan menghitung mortalitas larva Aedes aegypti dalam setiap jam pengamatan. Hasil penelitian menunjukkan bahwa, perlakuan dengan ekstrak biji mahoni (Switenia macrophylla) konsentrasi 4,5% menyebabkan mortalitas larva mencapai 100%, sedangkan ekstrak sereh (Andropogon nardus) konsentrasi 4,5% menyebabkan mortalitas larva tertinggi (98%) dalam 24 jam pemaparan. Pada kombinasi ekstrak tanaman sereh (Andropogon nardus) dan biji mahoni (Switenia macrophylla) dengan konsentrasi masing-masing 3.0% +3.0% dan  4,5%+4.5% menyebabkan mortalitas larva mencapai 100%. Berdasarkan WHO (2009), perlakuan ekstrak tanaman sereh (Andropogon nardus) dan ekstrak biji mahoni (Switenia macrophylla) dengan konsentrasi tersebut efektif sebagai larvasida untuk larva Aedes aegypti. Kata kunci: Mortalitas, larva Aedes aegypti, ekstrak sereh (Andropogon nardus), ekstrak biji mahoni (Switenia macrophylla). ABSTRACT This study aims to determine the effect of sereh grass extract (Andropogon nardus), mahogany seeds (Switenia macrophylla) and a combination of both of them to the mortality of Aedes aegypti mosquito larvae. Aedes aegypti mosquito larvae for the sample were rearing F2 larvae obtained by ovitrap from the Kambu Village area which was an endemic area of Dengue Hemorrhagic Fever (DHF). This research was conducted at the Zoology laboratory, FMIPA UHO Kendari. Lemon grass extract (Andropogon nardus) and mahogany seed extract (Switenia macrophylla) each with a concentration of 1.0% and 3.0% and a combination of the two types of extract were exposed for 24 hours to see the mortality of Aedes aegypti mosquito larvae. Data collection was done by calculating the mortality of Aedes aegypti larvae in each observation hour. The results showed that treatment with mahogany seed extract (Switenia macrophylla) concentration of 4.5% caused larval mortality to reach 100%, while lemongrass extract (Andropogon nardus) concentration of 4.5% caused the highest larval mortality (98%) in 24 hours of exposure. The combination of lemongrass extract (Andropogon nardus) and mahogany seeds (Switenia macrophylla) with a concentration of 3.0%+3.0% and 4.5% + 4.5% caused larval mortality to reach 100%. Based on WHO (2009), the treatment of citronella extract (Andropogon nardus) and mahogany seed extract (Switenia macrophylla) at that concentration were effective  as larvacidal for larval Aedes aegypti mosqouitoes. Keywords: Mortality, Aedes aegypti, Plant Extracts of Lemon Grass (Andropogon nardus), Mahogany Seed Extract (Switenia macrophylla)

scholarly journals Hubungan Jumlah dan Kepadatan Penghuni Rumah serta Keberadaan Nyamuk dengan Frekuensi Menggigit Nyamuk Aedes aegypti Saat Mencari Darah di Kabupaten Cirebon Provinsi Jawa Barat

2010 ◽  
Vol 2 (2) ◽  
pp. 95-98
Author(s):  
Lukman Hakim ◽  
Asep Jajang Kusnandar
Keyword(s):  
Infectious Disease ◽  
Risk Factor ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Laboratory Tests ◽  
Hemorrhagic Fever ◽  
Mosquito Larvae ◽  
Residential Settings ◽  
Dengue Transmission

Dengue hemorrhagic fever (DHF) is an infectious disease caused by dengue virus with the widest spread in the Asian region. In Indonesia, every year is always an outbreak in some provinces, the largest occurred in 1998 and 2004 with the number of patient mortality by 79 480 people with 800 people. To know the risk factor for dengue transmission in Cirebon District that had a high DHF cases, had been conducted the research with calculating the number and density of humans at the houses and also survey of density of Aedes aegypti pre-adult and adult stages.The research was resulting that the house index (HI) of Ae. aegypti is 58%, while homes found Ae. aegypti adults is 46%, 6 of which house is the house that is not found larvae / pupae of mosquitoes. The laboratory tests found that the frequency of biting Ae. aegypti mosquitoes average 4.5 times per day, at least 2 times and no more than 7 times per day. Number of people bitten by adult mosquitoes average of 3.1 people per day, is at least 2 people and maximum 5 people per day. Based on the statistically test, it is known that there is significant correlation between the number of inhabitants of the house and the presence of mosquito larvae / pupa of Ae. aegypti mosquito with biting frequency, whereas the most dominant variable was the presence of larvae / pupae of mosquitoes. Furthermore, it is advisable to do the cleaning intensification of Aedes breeding places inand outside the home including those hidden. It is also necessary to place residential settings so that the number of occupants per house so less.

Difusi dan Pola Spasial Sebaran Penyakit Demam Berdarah Dengue (DBD) Di Kota Bandar Lampung

2018 ◽  
Vol 1 (1) ◽  
pp. 87-95
Author(s):  
Nurul Qamila ◽  
Agel Vidian Krama
Keyword(s):  
Public Health ◽  
Spatial Analysis ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Spatial Pattern ◽  
Dengue Hemorrhagic Fever ◽  
Health Problem ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Descriptive Method

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and is transmitted by the mosquito Aedes aegypti (Aa.aegypti). The population is still a public health problem that increases the number of sufferers and also widespread, with population and education. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the spatial pattern and the spread of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the descriptive method and spatial pattern of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. Keywords: DHF, Spatial Analysis

scholarly journals Effects of two formulations based on Bacillus thuringiensis subsp. israelensis on Aedes aegypti mosquito larvae under laboratory conditions

2008 ◽  
Vol 23 (4) ◽  
pp. 251-258
Author(s):  
Milenka Peric ◽  
Mirjana Prijovic ◽  
Goran Andric
Keyword(s):  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Aqueous Suspension ◽  
Pure Water ◽  
Clay Content ◽  
Experimental Period ◽  
Mosquito Larvae ◽  
Water Dispersible ◽  
Bacillus Thuringiensis Subsp ◽  
Zero Time

Toxicity and persistence of two formulations based on Bacillus thuringiensis subsp. israelensis applied to Aedes aegypti mosquito larvae were tested under laborabory conditions. The formulations were: a) water dispersible granules (product VectoBac WDG), and b) aqueous suspension (product VectoBac 12AS). The effects of both products on mosquito larvae were tested immediately after their dilution in pure water (zero time) and in 1-, 2-, 8- and 13-day old solutions. The two products were also tested in mixtures of water and clay at a rate of 0.5 g clay/150 ml water immediately after product dilution, and in one-day old solutions containing 0.1 and 0.05 g of clay in the same amount of water. The product VectoBac WDG was persistent and highly effective against Ae. aegypti larvae in pure water after the longest experimental period of 13 days, and significantly more effective than VectoBac 12AS at equal rates of application. The effectiveness of VectoBac 12AS weakened significantly after 8 and 13 days of treatment, compared to the effects at zero time and in 1- and 2-day old solutions. High clay content in water significantly reduced the larvicidal effectiveness of both products, indicating its possible compromising role during product application in practice.

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