Relationship of Environmental Condition, Container and Behavior with the Existence of Aedes aegypti Mosquito Larvae in an Endemic Area of Dengue Hemorrhagic Fever, Makassar

2016 ◽  
Vol 15 (3) ◽  
pp. 295-298
Author(s):  
Ashari Rasjid ◽  
Ririh Yudhastuti ◽  
Hari Basuki Notobroto
Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Eis Nurhiliya

ABSTRAK              Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak tanaman sereh (Andropogon nardus), biji mahoni (Switenia macrophylla) dan kombinasi keduanya terhadap mortalitas larva nyamuk Aedes aegypti. Larva nyamuk Aedes aegypti untuk sampel adalah larva hasil rearing F2 yang diperoleh dengan ovitrap dari wilayah Kelurahan Kambu yang merupakan wilayah endemis Demam Berdarah Dengue (DBD). Penelitian ini dilakukan di laboratorium Zoologi, FMIPA UHO, Kendari. Ekstrak tanaman sereh (Andropogon nardus) dan ekstrak biji mahoni (Switenia macrophylla) masing-masing dengan konsentrasi 1,5% dan 3,0% dan 4.5%. kombinasi kedua jenis ekstrak tersebut dipaparkan selama 24 jam untuk melihat mortalitas larva nyamuk Aedes aegypti. Pengumpulan data dilakukan dengan menghitung mortalitas larva Aedes aegypti dalam setiap jam pengamatan. Hasil penelitian menunjukkan bahwa, perlakuan dengan ekstrak biji mahoni (Switenia macrophylla) konsentrasi 4,5% menyebabkan mortalitas larva mencapai 100%, sedangkan ekstrak sereh (Andropogon nardus) konsentrasi 4,5% menyebabkan mortalitas larva tertinggi (98%) dalam 24 jam pemaparan. Pada kombinasi ekstrak tanaman sereh (Andropogon nardus) dan biji mahoni (Switenia macrophylla) dengan konsentrasi masing-masing 3.0% +3.0% dan  4,5%+4.5% menyebabkan mortalitas larva mencapai 100%. Berdasarkan WHO (2009), perlakuan ekstrak tanaman sereh (Andropogon nardus) dan ekstrak biji mahoni (Switenia macrophylla) dengan konsentrasi tersebut efektif sebagai larvasida untuk larva Aedes aegypti. Kata kunci: Mortalitas, larva Aedes aegypti, ekstrak sereh (Andropogon nardus), ekstrak biji mahoni (Switenia macrophylla). ABSTRACT This study aims to determine the effect of sereh grass extract (Andropogon nardus), mahogany seeds (Switenia macrophylla) and a combination of both of them to the mortality of Aedes aegypti mosquito larvae. Aedes aegypti mosquito larvae for the sample were rearing F2 larvae obtained by ovitrap from the Kambu Village area which was an endemic area of Dengue Hemorrhagic Fever (DHF). This research was conducted at the Zoology laboratory, FMIPA UHO Kendari. Lemon grass extract (Andropogon nardus) and mahogany seed extract (Switenia macrophylla) each with a concentration of 1.0% and 3.0% and a combination of the two types of extract were exposed for 24 hours to see the mortality of Aedes aegypti mosquito larvae. Data collection was done by calculating the mortality of Aedes aegypti larvae in each observation hour. The results showed that treatment with mahogany seed extract (Switenia macrophylla) concentration of 4.5% caused larval mortality to reach 100%, while lemongrass extract (Andropogon nardus) concentration of 4.5% caused the highest larval mortality (98%) in 24 hours of exposure. The combination of lemongrass extract (Andropogon nardus) and mahogany seeds (Switenia macrophylla) with a concentration of 3.0%+3.0% and 4.5% + 4.5% caused larval mortality to reach 100%. Based on WHO (2009), the treatment of citronella extract (Andropogon nardus) and mahogany seed extract (Switenia macrophylla) at that concentration were effective  as larvacidal for larval Aedes aegypti mosqouitoes. Keywords: Mortality, Aedes aegypti, Plant Extracts of Lemon Grass (Andropogon nardus), Mahogany Seed Extract (Switenia macrophylla)


Author(s):  
Bobby Fahmi Muldan Pahlevi ◽  
Tri Wulandari Kesetyaningsih

Dengue Hemorrhagic Fever (DHF) is transmitted by Aedes aegypti and Ae. albopictus. Aedes aegypti chooses to live indoors and Ae. albopictus in outdoors. Aedes aegypti has been proven to play a role in dengue transmission in urban areas. It is assumed that suburbs dominated by gardens, so it is possible that Ae. albopictus acts as a vector. The study aimed to analyze the relationship between Ae. albopictus and DHF incidence in suburban area. The study was conducted in Godean, Sleman Regency. 280 ovitrap were placed indoors and outdoors at across seven villages. Each village was represented by one hamlet, so there were 40 ovitrap of each hamlet. The proportion of ovitrap containing Ae. aegypti and Ae. albopictus larvae was calculated. The results showed that Ae. aegypti larvae was more indoors (78.50%) than outdoors (21.45%) in all villages and negatively correlates with DHF (p=0.036; r=-0.776). Aedes albopictus larvae is more commonly found in gardens (80.64%) than in homes (19.36%) and does not correlate with DHF (p = 0.702). It was concluded that in suburban endemic area of ​​Sleman Regency, Ae. aegypti larvae was more indoors and negatively correlated with DHF. Aedes albopictus larva was more in the garden and did not correlate with DHF incidence.


bionature ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Amirullah Amirullah ◽  
Nurhayu Malik ◽  
Rosmaya Rosmaya

Abstract. The study of the efficacy of betel leaf extract (Piper betle L.) and garlic extract (Allium sativum) on the mortality of Aedes aegypti mosquito larvae was carried out at the Zoology Laboratory of the Faculty of Mathematics and Natural Sciences, Halu Oleo Kendari University. Aedes aegypti mosquito larvae were obtained from the rearing of F2 from Aedes aegypti eggs that were taken by ovitrap from in Kambu Village, Kendari City, which is an endemic area of Dengue Hemorrhagic Fever (DHF). Extrac of betel leaf (Piper betle L.) and garlic extract (Allium sativum) at concentrations of 1.0%, 2.0% and 3.0% respectively and their combinations were given to Aedes aegypti larvae (L4). The results showed that the highest larval mortality (88%) occurred after 24 hours of exposure to betel leaf extract (Piper betle L.) at a concentration of 3%, whereas with garlic extract (Allium sativum) the highest larval mortality (98%) occurred at concentration 3 %, and for the highest larval mortality (99%) found in a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum). Based on WHO (2009), the most effective treatment for killing Aedes aegyti larvae is 3% garlic (Allium sativum) extract and a combination of 3% betel leaf extract (Piper betle L.) and 3% garlic extract (Allium sativum).Keywords:  Biopesticide, Mortality, Aedes aegypti, betle leaf (Piper betle L.), garlic (Allium sativum).


2019 ◽  
Vol 12 (1) ◽  
pp. 46-53
Author(s):  
Sri Nadyar Ekawati ◽  
Nova Hariani ◽  
Sudiastuti Sudiastuti

AbstrakPengendalian vektor penyakit demam berdarah (DBD) di Indonesia menggunakan larvasida temephos telah berlangsung lebih dari 30 tahun, oleh karena itu, perlu dilakukan penelitian untuk mengetahui efektivitas temephos dan Bacillus thuringiensis var. israelensis yang merupakan larvasida jenis baru dari agen biologi terhadap larva nyamuk Aedes aegypti. Penelitian ini bertujuan untuk mengetahui perbandingan efektivitas temephos dengan B. thuringiensis var. israelensis terhadap mortalitas larva nyamuk Ae. aegypti dari tiga Kecamatan di Samarinda, yaitu Kecamatan Samarinda Utara Kelurahan Gunung Lingai, Samarinda Ulu Kelurahan Air Putih dan Sungai Kunjang Kelurahan Loa Bakung. Metode yang digunakan adalah metode bioassay. Hasil penelitian menunjukan bahwa temephos tidak efektif untuk membunuh larva nyamuk Ae. aegypti dari tiga kelurahan yang diamati, dengan LC50,24jam sebesar 1,88–2,24 ppm dan LC90,24jam sebesar 2,07–3,59 ppm. Sementara itu, B. thuringiensis var. israelensis masih efektif untuk membunuh larva nyamuk Ae. aegypti dari ketiga kelurahan yang diamati, dengan LC50,24jam sebesar 0,93–1,00 mL/50 L air dan LC90,24jam sebesar 1,05–1,11 mL/50 L air. Hal ini berarti penggunaan B. thuringiensis var. israelensis dengan dosis yang dianjurkan pemerintah masih efektif untuk mengendalikan populasi nyamuk Ae. aegypti.AbstractThe control of dengue hemorrhagic fever (DHF) vector in Indonesia using larvicide temephos has been ongoing for more than 30 years. Hence, it is necessary to assess the effectiveness of temephos compared to Bacillus thuringiensis var. israelensis which is a new type of biological larvacide agent against Aedes aegypti mosquito larvae. This study aimed to determine the effectiveness of temephos compared to B. thuringiensis var. israelensis against mortality of Ae. aegypti mosquito larvae sampled from three subdistricts in Samarinda namely Samarinda Utara Subdistrict Gunung Lingai, Samarinda Ulu Subdistrict Air Putih, and Sungai Kunjang Subdistrict Loa Bakung. The method used was bioassay. The results showed that temephos was not effective in killing Ae. aegypti mosquito larvae from the three subdistricts observed, with LC50.24 hours by 1.88–2.24 ppm, and LC90,24 hours by 2.07–3.59 ppm. Meanwhile, B. thuringiensis var. israelensis is still effective in killing Ae. aegypti mosquito larvae from the three subdistricts observed, with LC50.24 hours by 0.93–1.00 mL/50 L of water and LC90,24 hours by 1.05–1.11 mL/50 L of water. Those results mean that the application of B. thuringiensis var. israelensis with the recommended dosage of the government is still effective in controlling the population of Ae. aegypti.


2018 ◽  
Vol 7 (2) ◽  
pp. 113-119
Author(s):  
Dessy Triana ◽  
Sitti Rahmah Umniyati ◽  
Budi Mulyaningsih

Abstract Dengue Hemorrhagic Fever (DHF) is a disease caused by dengue virus and transmitted by Aedes aegypti mosquito as the main vector and Aedes albopictus as secondary vector. In 2016, Bengkulu City was one of 3 cities that experiencing DHF outbreaks in Indonesia. Insecticides malathion have been used since 1990 in bulk in DHF control programs in Bengkulu City and have not had an evaluation report on Aedes sp. vulnerability to malathion. The purpose of this research was to know the resistance status of Ae albopictus mosquitoes from endemic and sporadic areas of DHF in Bengkulu City to malathion. The sample of the study was the Ae. albopictus adult female mosquitoes which collected from endemic and sporadic areas of DHF in Bengkulu City with ovitrap installation. The resistance test was performed by CDC Bottle Bioassay method with malation 96% and 50 μg/ml diagnostic dose of. The results of this study showed 1% mortality on endemic area and 5% on sporadic area. Aedes albopictus of both areas were resistant to malathion.   Keywords: Ae. albopictus, bottle bioassay, malathion.   Abstrak Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh virus dengue dan ditularkan oleh nyamuk Aedes aegypti sebagai vektor utama dan Aedes albopictus sebagai vektor sekunder. Pada tahun 2016, Kota Bengkulu merupakan 1 dari 3 kota yang mengalami KLB DBD di Indonesia. Insektisida malation telah digunakan sejak tahun 1990 secara massal dalam program pengendalian DBD di Kota Bengkulu dan belum memiliki laporan evaluasi mengenai kerentanan Aedes sp terhadap malation. Tujuan penelitian untuk mengetahui status kerentanan nyamuk Ae. albopictus dari daerah endemis dan sporadis DBD Kota Bengkulu terhadap malation. Sampel penelitian adalah nyamuk betina dewasa Ae. albopictus yang dikoleksi dari daerah endemik dan sporadis DBD Kota Bengkulu dengan pemasangan ovitrap. Uji kerentanan dilakukan dengan metode CDC Bottle Bioassay dengan malation 96% dan dosis diagnostik 50 µg/ml. Hasil penelitian ini  menunjukkan mortalitas 1% untuk daerah endemis dan 5% untuk daerah sporadis. Aedes albopictus kedua daerah telah resisten terhadap malation.   Kata Kunci: Ae. albopictus, bottle bioassay, malation.


Author(s):  
Revi Rosavika Kinansi ◽  
Aryani Pujiyanti

Dengue Hemorrhagic Fever (DHF) is influenced by the density of disease vectors. One of government program to reduce dengue cases in Indonesia is the Eradication of Mosquito Nest (PSN) through monitoring of Water Reservoirs (TPA). This study aims to determine the opportunities for TPA characteristics to influence the presence of Aedes sp. larvae in 19 provinces in Indonesia in the Riset Khusus Vektor dan Reservoir Penyakit in 2015 and 2016. The study was conducted cross sectionally in 570 areas near settlements which are dengue endemic areas and each province was taken three districts that have one DHF endemic area. The data obtained in the form of landfill characteristics variables and the presence of larvae of Aedes sp. analyzed descriptively and logistic regression. The analysis showed that 88% of the landfill contained larvae. Almost all the characteristics of the landfill examined have an effect on the existence of larvae with an average odd ratio of 3.2. Properly done landfill drainage significantly reduces the chances of mosquito eggs hatching into larvae by 11,843 times more than landfills that are rarely drained. Maintaining fish larvae in the landfill has a significant effect in reducing the population of mosquito larvae by 4.937 times. Sowing container with larvasida has the opportunity to reduce mosquito larvae by 4.483 times. The results of this study can be used as a basis for DHF control efforts in communities in endemic areas.


2019 ◽  
Vol 10 (02) ◽  
pp. 21376-21384
Author(s):  
I Gusti Agung Gede Sudarmika ◽  
I Wayan Maba ◽  
I Ketut Widnyana

Dengue hemorrhagic fever (DHF) is a disease that is still a problem in Indonesia, including Bali. The Padangbai Karangasem port area is one of the entrances to countries that are not yet free from dengue fever cases, because there are still reported cases of dengue fever. The number of House of Aedes Flicking Index is still more than 1%. Based on Minister of Health Decree number 431 of 2007 Port Buffer Area <1%. The implementation of the Mosquito Nest Eradication through 3M Plus is the most effective effort to prevent the transmission of Dengue Hemorrhagic Fever.The purpose of this study was to determine the relationship between knowledge, attitudes and infrastructure with the behavior of implementing the Eradication of Dengue Hemorrhagic Fever Nests. The research design was quantitative with a cross sectional design. This research was conducted in the Padangbai Karangasem Port Area, Bali. Data collection used a questionnaire about Knowledge, Attitudes,facilities and infrastructure and Behavior for Eradicating Dengue Hemorrhagic Fever Eradication. The number of samples of 89 respondents was taken proportional random sampling. The results showed that Knowledge, Attitudes and Infrastructure were significantly related to the eradication behavior of dengue fever mosquitoes due to the value of p<α=0.,05. The most dominant variable associated with the implementation behavior of Eradication of Dengue Hemorrhagic Fever Eradication is Knowledge Variable because the highest OR value is 4.287 compared to Attitudes and infrastructure.


2021 ◽  
Vol 16 (1) ◽  
pp. 23
Author(s):  
Rahmad Wahyudi ◽  
Harfina Indriani ◽  
Yogi Abror

Dengue hemorrhagic fever (DHF) is still a serious health problem in the community. DHF is caused by the dengue virus (DENV) and is transmitted by the Aedes aegypti (Ae. aegypti) mosquito, which is the main vector for the virus. In controlling DHF vectors, larvicides are still used. These larvicides contain chemical compounds that have negative side effects affecting      the human body. Therefore, there is a need for natural-based larvacides (biolarvicides). The purpose of this study was to determine the potential of corn silk as a biolarvicide against dengue vector larvae (Aedes aegypti). This study was carried out at the STIKes Ngudia Husada Madura Bangkalan Laboratory and was done by taking corn silk waste samples disposed of as waste from corn farmers and corn traders in Bangkalan Regency. A Thousand samples of Aedes aegypti larvae that had reached instar III were used and divided into five test groups, namely 0 g/L (control), 6.25 g/L, 12.5 g/L, 25 g/L, and 50 g/L . Each group contained 200 larvae. The experiment was repeated 3 times. Data of the number of deaths of Aedes aegypti larvae were collected every 24 hours. The highest dose that could kill Aedes aegypti larvae was at 20 g/L.Keywords     : biolarvicides, corn silk, larvae, Aedes aegypti


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