scholarly journals Immunological evaluation of inactivated Newcastle disease vaccine depending on adjuvant composition

2021 ◽  
Vol 12 (3) ◽  
pp. 490-497
Author(s):  
A. I. Chegrynets ◽  
О. О. Saliy ◽  
I. A. Sobko ◽  
V. O. Krasinko
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Genetic Material ◽  
Tween 80 ◽  
Positive Control ◽  
Control Group ◽  
Chicken Embryos ◽  
Commercial Vaccine ◽  
The Impact ◽  
Oil Type

Newcastle disease is a global problem that is being recorded in most countries and also a serious obstacle to exchange of genetic material of poultry in various countries of the world. Control of the Newcastle disease comprises correct injection of efficacious vaccines so as to decrease or eliminate the clinical disease. Our goal was to perform comparative studies of the vaccines against Newcastle disease of water in oil type, the adjuvant being mineral oil mixed with emulsifiers (Span-80 and Tween-80) and ready-to-use adjuvant system (Montanide ISA 70), and study the impact of composition of adjuvant constituent on physical-chemical and immunogenic properties of inactivated vaccines. To reproduce virus-containing material and carried out titration of the viruses, we used chicken embryos free of pathogenic microflora. Aqueous phase for the preparation of emulsion-based vaccines of water in oil type consisted of antigen to Newcastle disease of La-Sota strain, manufactured by Biotestlab Ltd, and phosphate-saline buffer. To evaluate the effectiveness of the vaccine and induce immune response, we used 1-day old pathogen-free chickens, which were obtained from chicken embryos free of pathogenic microflora. As the positive control in the experiment, we used commercial vaccine. One-day chickens were divided into 3 groups (I, II, III) comprising 12 individuals each and one group (IV) consisting of 8 individuals as the control group with individual numeration. Chickens in groups I, II and III were divided into two subgroups (n = 8 and n = 4) to determine immunogenic efficiency and safety of the vaccine. Immunization was carried out through single subcutaneous injections in the region of the neck. To study immunogenic efficiency, the chickens were immunized with the dose of 0.1 mL (1 dose), and 0.2 mL (2 doses) to determine safety. After the immunization of 1-day old pathogen-free chickens with 0.1 mL dose, the obtained level of antibodies in the serum of vaccinated chickens on days 14, 21, 28, 35 and 42 after the vaccination indicated the ability of provoking the immune response to Newcastle disease at high level and safety of the vaccination for chickens. All the recipes of the examined series of the vaccines and the commercial vaccine produced appropriate level of viscosity according to the criterion equaling ≤ 200 mm2/s at Р <0.05, promoting fluidity of the vaccine and providing easier passage through the needle during the application. Both of the studied vaccines may be used in poultry farming for prophylaxis of Newcastle disease among chickens.

scholarly journals EFFECT OF VITAMIN E AND COD LIVER OIL SUPPLEMENT WITH BIVALENT OIL BASED VACCINE OF NEWCASTLE DISEASE AND INFECTIOUS BRONCHITIS DISEASE ON IMMUNE RESPONSE OF THE BROILERS.

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
ABDULWAHID & et al.
Keyword(s):  
Immune Response ◽  
Vitamin E ◽  
Newcastle Disease ◽  
Challenge Test ◽  
Elisa Test ◽  
The Other ◽  
Control Group ◽  
Broiler Chicks ◽  
Cod Liver Oil ◽  
Infectious Bronchitis

This study was aimed to enhance the immune response of the broilers by vitamin E and Cod liver oil supplement with locally inactivated bivalent vaccine of Newcastle disease and Infectious bronchitis viruses. One hundred and twenty broiler chicks Ross 308 were divided to four groups at 30 chicks of each group. The 2nd group was vaccinated with 0.5ml/chick of locally inactivated ND-IB vaccine at 5 days old. The 3rd group was vaccinated with 0.5ml locally ND-IB vaccine and 0.1 ml/chick of VE. The 4th group was vaccinated with 0.5ml of locally ND-IB vaccine and 0.1ml/chick of Cod liver oil, while the first group was left without vaccination as a control group. All groups were vaccinated with attenuated ND-IB at 3 days old and attenuated IBD at 14 days old. The results of ELISA test revealed that the 3rd and 4th groups showed significantly increased (P≤0.05) in Ab titers against ND virus at aged 35 days, also, the 4th group showed significantly (P≤0.05) increased in Ab titers against IB virus at day 35 of age as compared with the other groups. The 4th group showed a significant (P≤0.05) increased in bursal index as compared with the other groups. The results of challenge test revealed that vaccinated groups resist the velogenic ND and IB viruses with relatively high productive range particularly the 4th group as compared with other groups. In conclusion, the VE and Cod liver oil supplement with local bivalent oil based vaccine of ND and IBV may enhance the immune response of the broilers particularly the 4th group which vaccinated with 0.5 ml of local bivalent oil based vaccine of ND-IB vaccine and 0.1 ml Cod liver oil.

scholarly journals Silane Effect of Universal Adhesive on the Composite–Composite Repair Bond Strength after Different Surface Pretreatments

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 950 ◽  
Author(s):  
Gioia Michelotti ◽  
Maria Niedzwiecki ◽  
Darius Bidjan ◽  
Phoebe Dieckmann ◽  
Shengjile Deari ◽  
...  
Keyword(s):  
Bond Strength ◽  
Silica Coating ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Universal Adhesive ◽  
Diamond Bur ◽  
Composite Substrates ◽  
The Impact ◽  
Mechanical Surface

This study investigated the impact of a separate silanization step on the repair bond strength of composite substrates using a universal adhesive after various mechanical surface treatments. Composite specimens were aged and exposed to the following mechanical roughening treatments: diamond bur abrasion, aluminum oxide sandblasting, or silica coating. The specimens were then either left untreated or conditioned with universal adhesive (Scotchbond Universal), or a silane coupling agent was applied before the use of the universal adhesive or a conventional adhesive (Optibond FL). The conditioned surfaces and fresh substrate (positive control group) were covered with repair composite, and microtensile bond strength testing was performed. The significantly highest bond strength was obtained in the positive control group. Repair bond strength of the universal adhesive applied after a separate silanization step was similar to that without prior silanization, independent of the mechanical surface treatment. Moreover, after silica coating, no significant differences in the repair bond strength were detected among the different adhesive treatments. In conclusion, a separate silanization step before surface conditioning with the universal adhesive does not enhance the repair bond strength. On silica-coated composite substrates, repair bond strength values of the universal adhesive were similar to those of the conventional adhesive.

scholarly journals Immunogenicity of a new enhanced tetanus-reduced dose diphtheria-acellular pertussis (Tdap) vaccine against Bordetella pertussis in a murine model

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Kyu Ri Kang ◽  
Dong Ho Huh ◽  
Ji Ahn Kim ◽  
Jin Han Kang
Keyword(s):  
Murine Model ◽  
Booster Vaccination ◽  
Humoral Response ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Bacterial Challenge ◽  
Commercial Vaccine ◽  
Reduced Dose ◽  
Tdap Vaccine

Abstract Background The necessity of the tetanus-reduced dose diphtheria-acellular pertussis (Tdap) vaccine in adolescence and adults has been emphasized since the resurgence of small-scale pertussis in Korea and worldwide due to the waning effect of the vaccine and variant pathogenic stains in the late 1990s. GreenCross Pharma (GC Pharma), a Korean company, developed the Tdap vaccine GC3111 in 2010. Recently, they enhanced the vaccine, GC3111, produced previously in 2010 to reinforce the antibody response against filamentous hemagglutinin (FHA). In this study, immunogenicity and efficacy of the enhanced Tdap vaccine compared and evaluated with two Tdap vaccines, GC3111 vaccine produced in 2010 previously and commercially available Tdap vaccine in a murine model. Methods Two tests groups and positive control group of Balb/c mice were primed with two doses of the diphtheria-tetanus-acellular pertussis (DTaP) vaccine followed by a single booster Tdap vaccine at 9 week using the commercially available Tdap vaccine or 2 Tdap vaccines from GC Pharma (GC3111, enhanced GC3111). Humoral response was assessed 1 week before and 2 and 4 weeks after Tdap booster vaccination. The enhanced GC3111 generated similar humoral response compare to the commercial vaccine for filamentous hemagglutinin (FHA). The interferon gamma (IFN-γ) (Th1), interleukin 5 (IL-5) (Th2) and interleukin 17 (IL-17) (Th17) cytokines were assessed 4 weeks after booster vaccination by stimulation with three simulators: heat inactivated Bordetella pertussis (hBp), vaccine antigens, and hBp mixed with antigens (hBp + antigen). A bacterial challenge test was performed 4 weeks after booster vaccination. Results Regarding cell-mediated immunity, cytokine secretion differed among the three simulators. However, no difference was found between two test groups and positive control group. All the vaccinated groups indicated a Th1 or Th1/Th2 response. On Day 5 post-bacterial challenge, B. pertussis colonies were absent in the lungs in two test groups and positive control group. Conclusions Our results confirmed the immunogenicity of GC Pharma’s Tdap vaccine; enhanced GC3111 was equivalent to the presently used commercial vaccine in terms of humoral response as well as cell-mediated cytokine expression.

scholarly journals Evaluation of anti-ulcer activity of 4-hydrooxy benzalydehide against NSAIDs induced ulcers in rats

2021 ◽  
Vol 9 (5) ◽  
pp. 1412
Author(s):  
Rupali V. Jadhav ◽  
V. K. Redasani ◽  
Shankar B. Kalbhare ◽  
Karishma Yadav ◽  
Aryan Langeh ◽  
...  
Keyword(s):  
Low Dose ◽  
Positive Control ◽  
Gastric Volume ◽  
Control Group ◽  
High Dose ◽  
Antiulcer Activity ◽  
Ulcer Index ◽  
Pylorus Ligation ◽  
The Impact ◽  
Intermediate Dose

Background: The aim of this study was to evaluate antiulcer activity of 4-hydroxybenzaldehyde against NSAIDs induced ulcer in rats based differences in its morphology, distance with other external landmarks and also to sigmoid and transverse sinuses.Methods: The antiulcer activity of 4-HBD was evaluated using pylorus ligation-aspirin induced ulcer method. Animals of this models were treated with 4-HBD (50mg/kg, 100mg/kg and 150mg/kg).Results: It has been observed that 4-HBD at low dose (50mg/kg), intermediate dose (100mg/kg) and high dose (150mg/kg) showed significant increase in pH, significant decrease in gastric volume, significant decrease in ulcer index and significant decrease in total acidity.Conclusions: The impact of 4-HBD therapy with intermediate (100mg/kg, p.o.) dose was observed to be similar with the positive control group.  

scholarly journals Study of Using Thymbra spicata leaves to reduce the toxic immunosuppresive effect of aflatoxin in broilers

2008 ◽  
Vol 32 (1) ◽  
pp. 140-147
Author(s):  
Hatem M. M. Al-Naemey
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Suppressive Effect ◽  
The Third ◽  
The Impact ◽  
Thymbra Spicata ◽  
Newcastle Disease Vaccine ◽  
Protection Ratio

The present study performed to evaluate the impact of addition ofThymbra spicata leaves powder of aflatoxin contaminated ration inreduction of the aflatoxin immuno suppressive effect in the immuneresponse induced by Newcastle disease vaccine in broilers.The study conducted in three groups, The first group fed aflatoxin freeration, second group fed aflatoxin contaminated ration without anytreatment, while the third group fed aflatoxin contaminated rationsupplemented of 2% Thymbra spicata leaves powder.All chick groups were vaccinated twice with Newcastle disease vaccine(NDV) at 10 and 20 days of age. ELIZA test was carried out to estimate thehumeral immune response at 6, 19 and 31 days of age. Infection challengwere done at day 32 of age with velogenic ND strain (Z-2003).The results showed that the first group has high significante antibodies titerreach to 3239±249.3 at 19days of age and 10186+845.3 at 31 days of agewith significant level 0.01 and high protection ratio against challenge(100%)at 32 days of age, while the second group has the low antibodies titerreach to 2622.6±27.3 at 19 days of age and 5712.5±786.9at 31daysof ageand low protection ratio against challenge(66.67), the third group’santibodies titer and protection ratio lower than first group and higher thansecond group ,the titers are 3060±542.5 at 19 days of age and 5712.5±786.9at31 days of age while the protection ratio against challenge is 80% at 32days of age .The results showed the immuno suppressive effect of aflatoxin as in secondgroup and the role of Thymbra spicata leaves powder in reduction of theseeffect.

scholarly journals Effect of Mycoplasma synoviae strains isolated from the respiratory and reproductive tracts of chicken on SPF chicken embryos

2027 ◽  
Vol 74 (10) ◽  
pp. 6142-2027
Author(s):  
OLIMPIA KURSA ◽  
GRZEGORZ TOMCZYK ◽  
ANNA SAWICKA
Keyword(s):  
Respiratory Tract ◽  
Genetic Material ◽  
Economic Problem ◽  
Poultry Production ◽  
Mycoplasma Synoviae ◽  
Chicken Embryos ◽  
Microbiological Methods ◽  
Chicken Oviduct ◽  
Genotype C ◽  
The Impact

Mycoplasma synoviae (MS) infections in poultry are an important epidemiological and economic problem in poultry production all over the world. The differences between M. synoviae strains are related to the pathogenicity and the course of the disease. In recent years, the pathogenicity of M. synoviae strains has increased, and some of them are capable of causing serious infections. Both horizontal and vertical transmission routes play an important role in MS infection in flocks. The aim of the study was to determine the impact of infection with selected MS strains obtained from chickens showing a clinical form of MS infection on SPF chicken embryos. Ten strains of M. synoviae were used for this purpose. The strains were isolated from the respiratory tract and the oviduct of chickens with symptoms typical of infection with this pathogen. Genetic material isolated from liquid cultures of these strains was confirmed by molecular (PCR and LAMP) and microbiological methods. The selected M. synoviae strains belonged to six different genotypes. Significant differences in virulence between the strains were demonstrated. In nine infected groups of embryos, M. synoviae strains caused weight loss, and in seven groups they produced anatomopathological changes characteristic of mycoplasma infections. The most pathogenic for SPF chicken embryos turned out to be strains characterized as genotype F isolated from the chicken oviduct and strains of genotype C isolated from the respiratory tract. One strain of genotype H isolated from the respiratory tract showed no pathogenic effect on SPF chicken embryos. The study showed that infections with M. synoviae can have a significant impact on the production of chicken chicks in commercial hatcheries and the economy of the poultry industry.

scholarly journals Vaccination with the Chlamydia trachomatis Major Outer Membrane Protein Can Elicit an Immune Response as Protective as That Resulting from Inoculation with Live Bacteria

2005 ◽  
Vol 73 (12) ◽  
pp. 8153-8160 ◽  
Author(s):  
Sukumar Pal ◽  
Ellena M. Peterson ◽  
Luis M. de la Maza
Keyword(s):  
Immune Response ◽  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Major Outer Membrane Protein ◽  
Control Group

ABSTRACT BALB/c mice were vaccinated by the intramuscular (i.m.) and subcutaneous (s.c.) routes with a native preparation of the Chlamydia trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP), using Montanide ISA 720 and CpG-1826 as adjuvants. A negative control group was immunized with ovalbumin and the two adjuvants, and a positive control group was immunized intranasally (i.n.) with 104 inclusion-forming units (IFU) of C. trachomatis. Four weeks after the last i.m.-plus-s.c. immunization, mice were challenged in the ovarian bursa with 105 IFU of C. trachomatis MoPn. Six weeks after the genital challenge, animals were mated, and the pregnancies were monitored. After vaccination with MOMP, the mice developed strong Chlamydia-specific humoral and cellular immune responses. Following the genital challenge, of the mice vaccinated with the MOMP, only 15% (3/20) had positive vaginal cultures, while 85% (17/20) of the animals immunized with ovalbumin had positive cultures over the 6 weeks of observation (P < 0.05). Also, only 14% (3/21) of the animals inoculated i.n. with Chlamydia had positive vaginal cultures. After mating, 75% (15/20) of the mice vaccinated with MOMP carried embryos in both uterine horns. Of the animals vaccinated i.n. with the Chlamydia, 81% (17/21) had embryos in both uterine horns (P > 0.05). In contrast, only 10% (2/20) of the mice immunized with ovalbumin had embryos in both uterine horns (P < 0.05). In conclusion, immunization with a purified preparation of the MOMP is as effective as vaccination with viable C. trachomatis in eliciting a protective immune response against a genital challenge in mice.

scholarly journals The effect of passive exposure to tobacco smoke on the immune response in children with asthma 

2020 ◽  
Author(s):  
Agata Wawrzyniak ◽  
Agnieszka Lipinska-Opalka ◽  
Boleslaw Kalicki ◽  
Malgorzata Kloc
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Tobacco Smoke ◽  
Control Group ◽  
Study Group ◽  
Healthy Children ◽  
Immunomodulatory Effects ◽  
Passive Exposure ◽  
Children With Asthma ◽  
The Impact

Abstract IntroductionThere are a few publications about the impact of tobacco smoke on the children’s immune system. Material and MethodsThe study group consisted of 43 children with asthma.. The control group consisted of 37 healthy children. The exposure to tobacco smoke was assessed by the presence of the cotinine in the urine. ResultsThe group of children with asthma exposed to tobacco smoke had significantly higher levels of the IL-1 and lower levels IL-4 than children not exposed to the passive smoking. The children from the control group exposed to tobacco smoke had a significantly higher concentration of IL-4 than unexposed children. In the whole analyzed population, there was a significant correlation between the presence of cotinine and the concentration of IL-1 and CRP. ConclusionIn this study we found that the passive exposure to tobacco smoke has the immunomodulatory effects on the immune system.

scholarly journals Comparing Impact of Two Resin Infiltration Systems on Microhardness of Demineralized Human Enamel after Exposure to Acidic Challenge

2021 ◽  
Vol 9 (D) ◽  
pp. 92-97
Author(s):  
Ebaa Alagha ◽  
Mustafa Ibrahim Alagha
Keyword(s):  
Positive Control ◽  
Negative Control ◽  
Enamel Surface ◽  
Control Group ◽  
Single Bond ◽  
Universal Adhesive ◽  
Resin Infiltration ◽  
Demineralized Enamel ◽  
Insignificant Difference ◽  
The Impact

AIM: This study compared the impact of two resin infiltration systems on microhardness of demineralized enamel before and after an acidic challenge. MATERIALS AND METHODS: A total of forty human maxillary molar teeth were used in this study. Each tooth has 4 groups (four standardized windows onto each tooth). Group A1: Untreated sound enamel surface (positive control), Group A2: Artificially demineralized enamel surface (negative control), Group A3: Icon resin infiltrating to demineralized enamel, while Group A4: Single bond universal adhesive applied to the demineralized enamel surface. All teeth were immersed in a demineralizing solution. The groups (A3 and A4) were further subdivided into two subgroups according to acidic ethanol challenge Subgroup B1: Specimens tested before an acidic challenge and B2: Specimens tested after an acidic challenge. Vickers microhardness test was done for all groups. One-way analysis of variance (ANOVA) was used to study the difference between tested groups on mean microhardness within each group. Tukey’s post-hoc test was used for pair-wise comparison between the means when ANOVA test was performed, and the significance level was set at p ≤ 0.05. RESULTS: Icon resin infiltration and single bond universal adhesive showed significantly higher mean microhardness than negative control, but significantly lower mean microhardness than positive control. However, insignificant difference was found between icon and single bond universal adhesive. After the acidic challenge, icon resin infiltration showed significantly higher mean microhardness than negative control. However, single bond universal adhesive showed insignificant difference as compared to the negative control. CONCLUSION: After an acidic challenge, icon resin infiltration was more successful than single bond universal total-etch adhesive system in microhardness. RECOMMENDATION: Icon resin infiltration technique is a promising, noninvasive approach that prevents the progress of the carious lesion.

Sign in / Sign up

Export Citation Format

Share Document