scholarly journals Effect of Mycoplasma synoviae strains isolated from the respiratory and reproductive tracts of chicken on SPF chicken embryos

2027 ◽  
Vol 74 (10) ◽  
pp. 6142-2027
Author(s):  
OLIMPIA KURSA ◽  
GRZEGORZ TOMCZYK ◽  
ANNA SAWICKA
Keyword(s):  
Respiratory Tract ◽  
Genetic Material ◽  
Economic Problem ◽  
Poultry Production ◽  
Mycoplasma Synoviae ◽  
Chicken Embryos ◽  
Microbiological Methods ◽  
Chicken Oviduct ◽  
Genotype C ◽  
The Impact

Mycoplasma synoviae (MS) infections in poultry are an important epidemiological and economic problem in poultry production all over the world. The differences between M. synoviae strains are related to the pathogenicity and the course of the disease. In recent years, the pathogenicity of M. synoviae strains has increased, and some of them are capable of causing serious infections. Both horizontal and vertical transmission routes play an important role in MS infection in flocks. The aim of the study was to determine the impact of infection with selected MS strains obtained from chickens showing a clinical form of MS infection on SPF chicken embryos. Ten strains of M. synoviae were used for this purpose. The strains were isolated from the respiratory tract and the oviduct of chickens with symptoms typical of infection with this pathogen. Genetic material isolated from liquid cultures of these strains was confirmed by molecular (PCR and LAMP) and microbiological methods. The selected M. synoviae strains belonged to six different genotypes. Significant differences in virulence between the strains were demonstrated. In nine infected groups of embryos, M. synoviae strains caused weight loss, and in seven groups they produced anatomopathological changes characteristic of mycoplasma infections. The most pathogenic for SPF chicken embryos turned out to be strains characterized as genotype F isolated from the chicken oviduct and strains of genotype C isolated from the respiratory tract. One strain of genotype H isolated from the respiratory tract showed no pathogenic effect on SPF chicken embryos. The study showed that infections with M. synoviae can have a significant impact on the production of chicken chicks in commercial hatcheries and the economy of the poultry industry.

scholarly journals Immunological evaluation of inactivated Newcastle disease vaccine depending on adjuvant composition

2021 ◽  
Vol 12 (3) ◽  
pp. 490-497
Author(s):  
A. I. Chegrynets ◽  
О. О. Saliy ◽  
I. A. Sobko ◽  
V. O. Krasinko
Keyword(s):  
Immune Response ◽  
Newcastle Disease ◽  
Genetic Material ◽  
Tween 80 ◽  
Positive Control ◽  
Control Group ◽  
Chicken Embryos ◽  
Commercial Vaccine ◽  
The Impact ◽  
Oil Type

Newcastle disease is a global problem that is being recorded in most countries and also a serious obstacle to exchange of genetic material of poultry in various countries of the world. Control of the Newcastle disease comprises correct injection of efficacious vaccines so as to decrease or eliminate the clinical disease. Our goal was to perform comparative studies of the vaccines against Newcastle disease of water in oil type, the adjuvant being mineral oil mixed with emulsifiers (Span-80 and Tween-80) and ready-to-use adjuvant system (Montanide ISA 70), and study the impact of composition of adjuvant constituent on physical-chemical and immunogenic properties of inactivated vaccines. To reproduce virus-containing material and carried out titration of the viruses, we used chicken embryos free of pathogenic microflora. Aqueous phase for the preparation of emulsion-based vaccines of water in oil type consisted of antigen to Newcastle disease of La-Sota strain, manufactured by Biotestlab Ltd, and phosphate-saline buffer. To evaluate the effectiveness of the vaccine and induce immune response, we used 1-day old pathogen-free chickens, which were obtained from chicken embryos free of pathogenic microflora. As the positive control in the experiment, we used commercial vaccine. One-day chickens were divided into 3 groups (I, II, III) comprising 12 individuals each and one group (IV) consisting of 8 individuals as the control group with individual numeration. Chickens in groups I, II and III were divided into two subgroups (n = 8 and n = 4) to determine immunogenic efficiency and safety of the vaccine. Immunization was carried out through single subcutaneous injections in the region of the neck. To study immunogenic efficiency, the chickens were immunized with the dose of 0.1 mL (1 dose), and 0.2 mL (2 doses) to determine safety. After the immunization of 1-day old pathogen-free chickens with 0.1 mL dose, the obtained level of antibodies in the serum of vaccinated chickens on days 14, 21, 28, 35 and 42 after the vaccination indicated the ability of provoking the immune response to Newcastle disease at high level and safety of the vaccination for chickens. All the recipes of the examined series of the vaccines and the commercial vaccine produced appropriate level of viscosity according to the criterion equaling ≤ 200 mm2/s at Р <0.05, promoting fluidity of the vaccine and providing easier passage through the needle during the application. Both of the studied vaccines may be used in poultry farming for prophylaxis of Newcastle disease among chickens.

scholarly journals Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 629
Author(s):  
Walaa A. Husseiny ◽  
Abeer A. I. Hassanin ◽  
Adel A. S. El Nabtiti ◽  
Karim Khalil ◽  
Ahmed Elaswad
Keyword(s):  
Silver Nanoparticles ◽  
Placebo Treatment ◽  
Cellular Level ◽  
Breast Muscle ◽  
Transcriptional Level ◽  
Poultry Production ◽  
In Ovo ◽  
Chicken Embryos ◽  
Muscle Tissues ◽  
Myogenic Factor

The present study was conducted to investigate the effects of colloidal nanoparticles of silver (Nano-Ag) on the expression of myogenesis-related genes in chicken embryos. The investigated genes included the members of the myogenic regulatory factors family (MRFs) and myocyte enhancer factor 2A (MEF2A) genes. A total of 200 fertilized broiler eggs (Indian River) were randomly distributed into four groups; non-injected control, injected control with placebo, treatment I in ovo injected with 20 ppm Nano-Ag, and treatment II in ovo injected with 40 ppm Nano-Ag. The eggs were then incubated for 21 days at the optimum temperature and humidity conditions. Breast muscle tissues were collected at the 5th, 8th, and 18th days of the incubation period. The mRNA expression of myogenic determination factor 1 (MYOD1), myogenic factor 5 (MYF5), myogenic factor 6 (MYF6), myogenin (MYOG), and MEF2A was measured at the three sampling points using real-time quantitative PCR, while MYOD1 protein expression was evaluated on day 18 using western blot. Breast muscle tissues were histologically examined on day 18 to detect the changes at the cellular level. Our results indicate that myogenesis was enhanced with the low concentration (20 ppm) of Nano-Ag due to the higher expression of MYOD1, MYF5, and MYF6 at the transcriptional level and MYOD1 at the translational level. Moreover, histological analysis revealed the presence of hyperplasia (31.4% more muscle fibers) in treatment I (injected with 20 ppm). Our findings indicate that in ovo injection of 20 ppm Nano-Ag enhances the development of muscles in chicken embryos compared with the 40-ppm dosage and provide crucial information for the use of silver nanoparticles in poultry production.

scholarly journals Salmonella Bacteriophage Diversity According to Most Prevalent Salmonella Serovars in Layer and Broiler Poultry Farms from Eastern Spain

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1456
Author(s):  
Sandra Sevilla-Navarro ◽  
Pablo Catalá-Gregori ◽  
Clara Marin
Keyword(s):  
Point Of View ◽  
Poultry Production ◽  
Poultry Products ◽  
Production Type ◽  
Poultry Farms ◽  
Commercial Poultry ◽  
Salmonella Serovars ◽  
The Impact ◽  
Phage Isolation ◽  
Salmonella Phage

The exploration of novel nonantibiotic interventions in the field, such as the use of bacteriophages, is necessary to avoid the presence of Salmonella. Bacteriophages are a group of viruses widely distributed in nature, strictly associated with the prokaryotic cell. Researchers have demonstrated the success of phage therapy in reducing Salmonella counts in poultry products. However, the impact that phage concentration in the environment may have against certain Salmonella serovars is not well understood. Therefore, the aim of this study was to assess Salmonella phage prevalence in commercial poultry farms in terms of the production type: layers or broilers. The most prevalent Salmonella serovars isolated in poultry production were used for phage isolation. Salmonella specific phages were isolated from 141 layer and broiler farms located in the Valencia region during 2019. Analysis of the samples revealed that 100% presented Salmonella phages, the most prevalent being against serovar S. Enteritidis (93%), followed by S. Virchow (59%), S. Typhimurium (55%), S. Infantis (52%) and S. Ohio (51%). These results indicate that poultry farms could represent an important source of Salmonella phages. Nevertheless, further studies are needed to assess the epidemiology of phages against other serovars present in other countries and their diversity from the point of view of molecular studies.

scholarly journals Radical Response: Effects of Heat Stress-Induced Oxidative Stress on Lipid Metabolism in the Avian Liver

Antioxidants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 35
Author(s):  
Nima K. Emami ◽  
Usuk Jung ◽  
Brynn Voy ◽  
Sami Dridi
Keyword(s):  
Oxidative Stress ◽  
Heat Stress ◽  
Lipid Metabolism ◽  
Egg Production ◽  
Well Being ◽  
Enzymatic Reactions ◽  
Poultry Production ◽  
Hepatic Lipid Metabolism ◽  
Hepatic Lipid ◽  
The Impact

Lipid metabolism in avian species places unique demands on the liver in comparison to most mammals. The avian liver synthesizes the vast majority of fatty acids that provide energy and support cell membrane synthesis throughout the bird. Egg production intensifies demands to the liver as hepatic lipids are needed to create the yolk. The enzymatic reactions that underlie de novo lipogenesis are energetically demanding and require a precise balance of vitamins and cofactors to proceed efficiently. External stressors such as overnutrition or nutrient deficiency can disrupt this balance and compromise the liver’s ability to support metabolic needs. Heat stress is an increasingly prevalent environmental factor that impairs lipid metabolism in the avian liver. The effects of heat stress-induced oxidative stress on hepatic lipid metabolism are of particular concern in modern commercial chickens due to the threat to global poultry production. Chickens are highly vulnerable to heat stress because of their limited capacity to dissipate heat, high metabolic activity, high internal body temperature, and narrow zone of thermal tolerance. Modern lines of both broiler (meat-type) and layer (egg-type) chickens are especially sensitive to heat stress because of the high rates of mitochondrial metabolism. While this oxidative metabolism supports growth and egg production, it also yields oxidative stress that can damage mitochondria, cellular membranes and proteins, making the birds more vulnerable to other stressors in the environment. Studies to date indicate that oxidative and heat stress interact to disrupt hepatic lipid metabolism and compromise performance and well-being in both broilers and layers. The purpose of this review is to summarize the impact of heat stress-induced oxidative stress on lipid metabolism in the avian liver. Recent advances that shed light on molecular mechanisms and potential nutritional/managerial strategies to counteract the negative effects of heat stress-induced oxidative stress to the avian liver are also integrated.

scholarly journals Friend or Foe? Impacts of Dietary Xylans, Xylooligosaccharides, and Xylanases on Intestinal Health and Growth Performance of Monogastric Animals

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 609
Author(s):  
Jonathan T. Baker ◽  
Marcos E. Duarte ◽  
Debora M. Holanda ◽  
Sung Woo Kim
Keyword(s):  
Crop Residues ◽  
Structural Difference ◽  
Nutrient Release ◽  
Food Product ◽  
Degree Of Polymerization ◽  
Poultry Production ◽  
Intestinal Health ◽  
Sustainable Food ◽  
And Performance ◽  
The Impact

This paper discusses the structural difference and role of xylan, procedures involved in the production of xylooligosaccharides (XOS), and their implementation into animal feeds. Xylan is non-starch polysaccharides that share a β-(1-4)-linked xylopyranose backbone as a common feature. Due to the myriad of residues that can be substituted on the polymers within the xylan family, more anti-nutritional factors are associated with certain types of xylan than others. XOS are sugar oligomers extracted from xylan-containing lignocellulosic materials, such as crop residues, wood, and herbaceous biomass, that possess prebiotic effects. XOS can also be produced in the intestine of monogastric animals to some extent when exogenous enzymes, such as xylanase, are added to the feed. Xylanase supplementation is a common practice within both swine and poultry production to reduce intestinal viscosity and improve digestive utilization of nutrients. The efficacy of xylanase supplementation varies widely due a number of factors, one of which being the presence of xylanase inhibitors present in common feedstuffs. The use of prebiotics in animal feeding is gaining popularity as producers look to accelerate growth rate, enhance intestinal health, and improve other production parameters in an attempt to provide a safe and sustainable food product. Available research on the impact of xylan, XOS, as well as xylanase on the growth and health of swine and poultry, is also summarized. The response to xylanase supplementation in swine and poultry feeds is highly variable and whether the benefits are a result of nutrient release from NSP, reduction in digesta viscosity, production of short chain xylooligosaccharides or a combination of these is still in question. XOS supplementation seems to benefit both swine and poultry at various stages of production, as well as varying levels of XOS purity and degree of polymerization; however, further research is needed to elucidate the ideal dosage, purity, and degree of polymerization needed to confer benefits on intestinal health and performance in each respective species.

scholarly journals Intellectual property rights in Brazilian floriculture: innovations for the growth and development of the market

2017 ◽  
Vol 23 (3) ◽  
pp. 296 ◽  
Author(s):  
Antonio Helio Junqueira ◽  
Marcia Peetz
Keyword(s):  
Growth And Development ◽  
Ornamental Plants ◽  
Genetic Material ◽  
The United States ◽  
Consumer Market ◽  
International Trends ◽  
Recent Developments ◽  
Cultivated Species ◽  
The Impact ◽  
Sectoral Policy

One of the most important demands imposed by the consumer market on the Brazilian Productive Chain of Flowers and Ornamental Plants is the constant launching of innovations in cultivated species and varieties. Such innovations include the constant introduction of flowers and plants not yet grown and commercialized, both native and adapted exotic species, as well as transformations and changes in size, shape, coloring and conduction patterns and presentation of these goods to the market. Brazil does not have a relevant breeding and cultivation industry. In this sense, it is highly dependent on imports of genetic material developed by countries such as the Netherlands, Germany, Japan, the United States of America, Thailand, among others. Recent developments in the sectoral policy to protect the rights of genetic developers, in the development of internationally adequate legislation and in the control of the use and trade of cultivars, has allowed Brazil greater access to new genetic materials of high quality and in line with contemporary international trends in the consumption. This article aims to discuss the state of the art of protection of cultivars in Brazil, pointing to the advances that the legislation and the inspection have allowed in relation to the introduction of genetic innovations, evaluating the impact of these measures on the growth and development of the market of consumption of flowers and ornamental plants in the country.

scholarly journals The impact of functional food on the prevention and treatment of respiratory diseases

2020 ◽  
Vol 33 (4) ◽  
pp. 228-232
Author(s):  
Gabriela Widelska ◽  
Kamila Kasprzak-Drozd ◽  
Karolina Wojtunik-Kulesza ◽  
Anna Krajewska ◽  
Anna Oniszczuk
Keyword(s):  
Respiratory Tract ◽  
Functional Food ◽  
Causes Of Death ◽  
Chronic Obstructive ◽  
Respiratory Tract Diseases ◽  
Chronic Obstructive Pulmonary Disorder ◽  
Prevention And Treatment ◽  
Pulmonary Disorder ◽  
Ancient Times ◽  
The Impact

Abstract Globally, diseases of the lung are one of the main causes of death, and conventional therapies are often ineffective in dealing with this serious medical and sociological problem. Since ancient times, medicinal plants have been used in the treatment of respiratory tract diseases. Such plants show antitussive, muscle relaxing, bronchi dilation or cillary movement effects. Their usefulness has been confirmed by modern and current research. A medicinal plant that is also a functional food can open new areas in the prevention and treatment of respiratory tract diseases. In this review, information about the influence of functional food on preventing and treating asthma, chronic obstructive pulmonary disorder (COPD) and high-altitude sickness are presented.

scholarly journals Interdisciplinary approaches to the new paradigm of the global financial and monetary architecture after the Covid 19 pandemic crisis in correlation with the social problems in the EU countries

2021 ◽  
Vol 129 ◽  
pp. 01008
Author(s):  
Ghenadie Ciobanu ◽  
Raluca Florentina Cretu ◽  
Mihai Dinu ◽  
Florin Dobre
Keyword(s):  
Global Economy ◽  
Social Systems ◽  
National Level ◽  
Value Added ◽  
Economic Problem ◽  
New Paradigm ◽  
Interdisciplinary Approaches ◽  
The Social ◽  
The World ◽  
The Impact

Research background: How will the world change after the pandemic? What will be the trends of the global economy after the pandemic in the conditions of digital transformations and the impact of other cutting-edge technologies that will change both the global paradigms of the world economy and the global financial and monetary architecture? It is a problem both globally and in each country. Purpose of the article: In this article we aim to examine the processes of transformation of the financial architecture worldwide in the current conditions of financial-monetary globalization, but also of the revolutionary transformations of digitalization and cybersecurity of national, regional, and global financial systems. Research method: We start from the historical approach of the world financial and monetary phenomenon in correlation with the social evolutions. Another method of research is longitudinal: the study of the world financial and monetary phenomenon in time in the context of building the new paradigm of development at the global level with the transition of building paradigms at the national level. In this context, the statistical method and the method of collecting statistical information are also necessary. Findings & Value added: In the conditions when many countries face various serious problems of social, demographic, mass population migration, imbalances in labor markets, declining quality of life, the new international financial-monetary paradigms, but also regional and national ones demand to be correlated by promoting current policies and building economic, financial-monetary and social systems that correspond to solving these socio-economic problem.

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