scholarly journals ВМІСТ ЖИРНИХ КИСЛОТ В ЛІПІДАХ ФЕТАЛЬНИХ СТОВБУРОВИХ КЛІТИН КОТА

2016 ◽  
Vol 18 (3(70)) ◽  
pp. 136-141
Author(s):  
L.V. Kladnitskaya ◽  
A.J. Mazurkiewicz ◽  
V.V. Danchuk ◽  
S.V. Velichko ◽  
S.V. Midyk

Defined content of fatty acids in lipids cat stem cells derived from fetal primary material. Fetal stem cells (FSCs) cat treated by culturing primary material in the CO2 incubator containing 5% CO2, at a temperature 37°С in DMEM medium with the addition of 15 – 20% fetal bulls serum and 1% antibiotic–antimycotic. When confluent monolayer reached 70 – 80%, the cells are removed from the culture dishes and held subcultivation to reduce the heterogeneity of culture. The resulting stem cells are tested for fatty acid content by gas–liquid chromatography.Determination of fatty acids in lipids fetal stem cells conducted under SOST ISO 5508–2001. Sample preparation was performed according to ISO 150 5509–2002 in our modification. A mixture of methyl esters of fatty acids were analyzed on the gas chromatograph Trace GC Ultra with flame ionization detector for capillary column SPTM –2560, 100 m x 0.25 mm ID, 0.20 μm film (Supelco). Identification of fatty acids was performed using a standard sample Supelco 37 Сomponent FAME Mix. Performed quantitative assessment by spectrum of crystal planes valuation peaks methylated derivatives LCD and determine their content as a percentage of the total content of all the LCD.Investigated that the lipids contained cat FSCs short–, medium– and long–chain fatty acids. In the lipid fetal stem cells found cat 18 Number fatty acids from saturated – most of palmitatе (34.53 ± 0.58%), with monounsaturated – оleate (20.20 ± 0.93%), with polyunsaturated – linoleic acid (6.27 ± 0.01%). Least composed of lipids of cells found cis–8.11.14–eykozatriyenovoyi acid (0.03 ± 0.01%).The total content of saturated fatty acids in the lipid cat FSCs is 67.75, unsaturated fatty acids – 32.25%. Saturation ratio is 2.10. Monoyenic fatty acids identified in the number of 23.19%, and polyenic – 9,06%. The index value n3 fatty acids to n6 in lipids cat FSCs is 0.35. 

2016 ◽  
Vol 8 (2) ◽  
pp. 92
Author(s):  
Hamidou SENOU ◽  
Cai X. ZHENG ◽  
Gabriel SAMAKE ◽  
Mamadou B. TRAORE ◽  
Fousseni FOLEGA ◽  
...  

<p class="1Body">The methyl esters of fatty acids composition of the oil from <em>jatropha curcas</em> seeds were analyzed by gas chromatography-mass spectrometer GC-MS. Fourteen components were found to be representative with 99.52% of the total content of seed oils. The main constituents were unsaturated fatty acids (71.93%) and saturated fatty acids (27.59%). For the saturated fatty acids composition such as palmitic and stearic acid, the rate was 15.80% and 10.79%, respectively. Linoleic acid (39.58%) and oleic acid (30.41%) were obtained in highest concentration among the unsaturated fatty acids identified in the seeds oil of <em>Jatropha curcas</em> from Guizhou. This value also justifies the fluidity of the oil at room temperature. A high percentage of polyunsaturated fatty acids (39.58%) and a slightly lower rate of monounsaturated fatty acids (32.35%) were also observed. The seed oils profile of Guizhou <em>Jatropha curcas</em> presents the desirable fatty acid C14 to C18 and interesting features for the biodiesel production.</p>


Author(s):  
І. С. Данілова

Представлено дані щодо жирнокислотного складу м'яса равликів видів Helix pomatia, Helix aspersa maxima та Helix aspersa muller. Встановлено, що із 21 кислоти кожен дослідний вид равликів містить як насичені, так і ненасичені жирні кислоти. З насичених містяться капронова, пальмітинова, стеаринова, міристинова, арахінова, каприлова, лауринова, бегенова, капринова, гептадеканова, а із ненасичених – олеїнова, пальмітолеїнова, лінолева, омега-3, омега-6, арахідонова, ліноленова, ціс-5,8,11,14,17-ейкозапента-єнова, ціс-11,14-ейкозадієнова, ціс-13,16-доказадіє-нова, докозапентаєнова. The data on fatty acid composition of snail meat of the following species: Helix pomatia, Helix aspersa maxima and Helix aspersa muller are presented. Unsaturated fatty acids are fatty acids that contain at least one double bond in the chain of fatty acids. Unsaturated fatty acids have a low melting point and are liquid in consistency, are easier assimilated by the human body than saturated fatty acids. Saturated fatty acids are fats of animal origin, entering the body from meat products, oils, eggs, sausages and dairy products. They differ from other fats in that they remain solid even at room temperature. Saturated fats are needed for energy, they are involved in the structure of cells. That is why their surplus leads to overweight accumulation, as well as to an increase in cholesterol in the body, heart disease and even some types of cancer. If a person does not consume saturated fatty acids, the body will be able to synthesize them from another food. However, for the body it is also superfluous, so in small quantities such fats are needed. The research was done in accordance with the procedures described in the relevant State Standards of Ukraine “Determination of fatty acid spectrum – SSU ISO 5508-2001. Fats and oils of animal and vegetable origin. Gas chromatography analysis of methyl esters of fatty acids. Sample preparation – SSU ISO 5509-2002. Fats of  animal and vegetable origin and oils. Preparation of methyl esters of fatty acids”. Chromatographic analysis of fatty acids was performed on a Trace Ultra gas chromatograph with a flame-ionization detector, on a capillary column SP-2560 (Supelco). The limit of the method is 0.01%. We have formed three groups of different snail species that are used for food purposes: Helix pomatia collected in the wet weather and in the morning, Helix aspersa maxima and Helix aspersa muller got from the farm «SNAIL 2016» (Ukraine), for which we express our gratitude to the owner. Snails of each species were the same in size and weight. Since our data were first obtained not only in Ukraine but also globally, it was not possible to compare them with the data of other authors. The results were processed statistically. As a result of our studies on the content of fatty acids in the meat of snails it is found that the mass fraction of unsaturated fatty acids is most commonly found in Helix pomatia snail meat and amounts to 132.14 % up to the amount of fatty acids. It is this fact that indicates the nutritional value of these snails. Saturated fatty acids enrich the meat of Helix aspersa muller snails and reaches 33.96% to the sum of fatty acids, and also in these types of snails ω-3 fatty acids to ω-6 fatty acids is 1:4.3. Such a combination of ω-3 fatty acid to ω-6 fatty acid is best for a good assimilation and well-being of a person who will consume such meat. It should be noted that the total content of fatty acids in the meat of snails varies in the following ranges: Helix pomatia 158.29, Helix aspersa maxima 148.97, and Helix aspersa muller 139.78. Attention should be drawn to the ratio of unsaturated fatty acids, namely mono-, di- and polyunsaturated acids in meat of each type of snail. According to our data in the meat of snails: Helix pomatia reaches 15.68: 37.67: 78.79, Helix aspersa maxima – 21.23: 31.61: 66.22, Helix aspersa muller – 26.7: 24.45 : 54.67. However, if you compare the meat of snails with the meat of black African ostrich, the fatty acid content of these two animals is very different. Thus, snail meat contains up to 14.36% palmitic acid, 13.98% stearic acid, 26.54% oleic acid, 37.67% linoleic acid and, of course, ω-3 and ω-6 fatty acids 7.49% and 51.12% respectively, depending on the type of snail, while in ostrich meat these figures reach only a tenth or even hundredth share. Thus, it can be argued that Helix pomatia, Helix aspersa maxima and Helix aspersa muller can be used as a valuable source of saturated and unsaturated fatty acids. For the first time in Ukraine, we studied the fatty acid content of Helix pomatia, Helix aspersa maxima, Helix aspersa muller snails, which are edible species and are used as a delicacy. Snail meat is a valuable source of essential fatty acids. Of the 21 acids in each experimental species, the snail contains both saturated and unsaturated fatty acids. The saturated ones contain: kapron, palmitic, stearin, myristic, arachin, capryl, lauric, behenic, kaprinic, heptadecanic and unsaturated: oleinic, palmitoleic, linoleic, omega-3, omega-6, arachidonic, linolenic, cis-5,8, 11,14,17-eicosapentae-noic, cis-11,14-eicosadenic, cis-13,16-proxazytoic, docosapentaenoic.


Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1133
Author(s):  
Atique Ahmed Behan ◽  
Muhammad Tayyab Akhtar ◽  
Teck Chwen Loh ◽  
Sharida Fakurazi ◽  
Ubedullah Kaka ◽  
...  

The supplementation of rumen bypass fat (RBF) has remained one of the preferred approaches used to decrease undesirable saturated fatty acids (FA) and increase beneficial unsaturated FA in the meat. This study was planned to evaluate the influences of rumen bypass fats on meat quality, fatty acid and metabolic profiles in male Dorper sheep (n = 36) with 24.66 ± 0.76 kg (mean ± standard error) initial body weight. Treatment comprised a basal diet (30:70 rice straw to concentrate) with no added RBF as a control (CON), basal diet with prilled fat (PF), basal diet with prilled fat plus lecithin (PFL) and basal diet with calcium soap of palm fatty acids (CaS). The findings revealed that cooking loss, drip loss and shear force in longissimus dorsi (LD) muscle were not affected by RBF supplementation, while meat pH was significantly higher in the CaS on aging day 1. However, the diet supplemented with prilled fat and lecithin modified the meat’s fatty acid profile significantly by increasing unsaturated fatty acids and decreasing saturated fats. The relative quantification of the major differentiating metabolites found in LD muscle of sheep showed that total cholesterol, esterified cholesterol, choline, glycerophosphocholine and glycerophospholipids were significantly lower in CaS and PFL diets, while glycerol and sphingomyelin were significantly higher in CaS and PFL diets. Most of the metabolites in the liver did not show any significant difference. Based on our results, the supplementation of protected fats did not have a negative influence on meat quality and the meat from Dorper sheep fed prilled fat with lecithin contained more healthy fatty acids compared to other diets.


2009 ◽  
Vol 2009 ◽  
pp. 212-212
Author(s):  
S J Hosseini Vashan ◽  
N Afzali ◽  
A Golian ◽  
M Malekaneh ◽  
A Allahressani

Palm oil is the most abundant of all oils produced globally. It is very high in saturated fatty acids specifically palmitic acid, but other fatty acids (monounsaturated (MUFA) and polyunsaturated) are presented at low concentrations. In the processing plant some high amount of oleic acid with some other unsaturated fatty acids are extracted and marketed as Palm olein oil, and used to reduce blood or egg cholesterol (Rievelles et al., 1994). The objective of this study was to determine the optimum level of dietary palm olein oil required to enrich the mono-unsaturated fatty acid content of yolk, egg cholesterol and antibody titre.


1982 ◽  
Vol 243 (2) ◽  
pp. G127-G133
Author(s):  
L. M. McLeay ◽  
J. M. Fitzgerald

Effects on ovine gastric function of procedures that increase intestinal unsaturated fatty acid content are unknown, and the present aim was to compare the effects of duodenal unsaturated and saturated fatty acids on gastric secretion in conscious sheep. During the maximal gastric secretory response to a meal, 10 ml gallbladder bile alone or with myristic, palmitic, and stearic acids and oleic, linoleic, and linolenic acids were infused into the duodenum at a rate of 5 g fatty acid . h-1 for 1 h. Compared with control 154 mM NaCl (100%), acid output was reduced to 4-7% of control with infusion of oleic, linoleic, and linolenic acids and myristic acids plus bile. Reductions in acid secretion persisted for up to 5 h from the end of infusion. In contrast, the infusion of palmitic and stearic acids with bile caused mean maximal reductions in acid output, respectively, to only 64 and 55% of control, and levels returned to control within 1 h of the end of infusion. Bile infusion alone caused no reduction in acid secretion. Under the conditions used, C18 unsaturated fatty acids and myristic acid were potent inhibitors of ovine gastric acid secretion. The lesser effects of palmitic and stearic acids were probably related to their reduced solubility and absorption.


2013 ◽  
Vol 67 (3) ◽  
Author(s):  
Adriána Bednárová ◽  
Ján Mocák ◽  
Walter Gössler ◽  
Margit Velik ◽  
Josef Kaufmann ◽  
...  

AbstractThe main aim of the present study was to find differences in the content of fatty acids and variations in elemental composition in beef samples of longissimus dorsi muscle related to cattle age and gender. A further goal was to describe interrelations among the selected variables (descriptors) characterising the samples. For this purpose, an extensive data table was compiled, which contains chemical descriptors specifying forty-six beef samples originating from four well-known Austrian grassland-based beef labels. The following descriptors were investigated: (a) concentrations of 33 fatty acids, (b) concentrations of 19 elements, (c) contents of dry-mass, protein, intramuscular fat, and ash, (d) total content of saturated fatty acids (SFA), mono-unsaturated fatty acids (MUFA), and poly-unsaturated fatty acids (PUFA), (e) total contents of omega-3 (n-3) and omega-6 (n-6) PUFA and their ratio. The correlation analysis provided a number of statistically significant correlations among the descriptors, which were concordant with the results of the principal component analysis and cluster analysis. Furthermore, the effect of age and gender of cattle (both acting as target factors) on the fatty acid content and elemental composition of beef was examined by analysis of variance (ANOVA) and appropriate non-parametric tests. Several important interrelations among the beef characteristics investigated were also discovered. Finally, the most relevant beef descriptors were utilised in linear discrimination analysis (LDA) for predicting the slaughter age of the cattle for beef authentication.


1963 ◽  
Vol 41 (1) ◽  
pp. 605-612 ◽  
Author(s):  
Joyce L. Beare ◽  
J. A. Campbell ◽  
C. G. Youngs ◽  
B. M. Craig

The effects of increasing the saturated fatty acids in a dietary vegetable oil composed mostly of unsaturated fatty acids were studied in rats. A mixture of palm oil and Swedish rapeseed oil fed for 4 weeks as 20% of a purified diet promoted weight gains which exceeded those obtained with Polish rapeseed oil of a similar content of erucic acid, and altered the proportion of saturated fatty acids in the tissues to reflect that of the diet. When methyl esters of saturated fatty acids were added to Swedish rapeseed oil, similar effects on weight gain were not observed, but methyl esters of fatty acids from corn oil and rapeseed oil were shown to be of less nutritional value than the original glycerides. From fatty acids of olive oil, glycerides containing 3% palmitic acid were prepared, and produced weight gains which did not differ significantly from those of rats fed Polish rapeseed oil with a similar content of palmitic acid and 20% erucic acid. The characteristic effects of rapeseed oil are, therefore, attributed to its low content of saturated fatty acids as well as its high content of erucic acid.


Author(s):  
Nataliya А. Politaeva ◽  
Irina V. Atamanyuk ◽  
Yuliya А. Smyatskaya ◽  
Tatiana A. Kuznetsova ◽  
Toumi Amira ◽  
...  

In the present article we introduce application areas of Сhlorella sorokiniana microalgae biomass for national economy (feed and biologically active additives, etc). Here it is shown that thermal and chemical modification of plant material waste might result in obtaining of highly-efficient materials for water purification. Major stages of waste-free technology of microalga processing with further extraction of valuable lipids and obtaining of sorption materials are schematically shown. We have determined fatty-acid content of lipid fraction (77 mg/g), obtained by Soxhlet method from lyophilized microalga biomass. It was revealed, that it includes 83.7% of unsaturated fatty acids (generally, С18:1, С18:2, С18:3), whereas saturated fatty acids are mostly presented by С16 – С20 family. In lyophilized biomass we discovered α-linolenoic acid (Omega-3, 28.3%), which is one of the most valuable biologically active compounds, required for full-value human life and activities. Microstructure analysis of residual biomass of C. sorokiniana microalgae has shown pore surface, formed by destroyed cells. In the course of studying the sorption properties of residual biomass with the use of initial and final concentration parameters, the efficiency of purification of model water from Fe3+ ions (47.5%) was evaluated, which indicates the need to modify the sorption properties of biomass. It has been established that the degree of wastewater purification containing iron (III) compounds can be significantly increased (by 1.8 times) by forming from the residual biomass granular materials with additional introduction of chitosan in the composition, which using as a binder and a modifying additive (mass ratio 4:1).


2000 ◽  
Vol 63 (2) ◽  
pp. 216-221 ◽  
Author(s):  
MANUELA E. PINTADO ◽  
F. XAVIER MALCATA

Vacuum packaging was assayed at 4°C and was tested in comparison to unpackaged counterparts, in both microbiological and physicochemical terms, in studies pertaining to the preservation of Requeijão, a traditional Portuguese whey cheese. Bacteria were absent (i.e., &lt;10 CFU/g) in whey cheeses on the day of manufacture as a result of thermal processing. After storage, both unpackaged and packaged cheeses exhibited high viable counts of Bacillus, Pseudomonas, Enterobacteriaceae, and lactic acid bacteria (especially lactococci). Yeasts, staphylococci, enterococci, and spore-forming clostridia were severely inhibited by the package vacuum combined with the increasing acidification developed therein. Whey cheeses packaged under vacuum underwent substantial acidification, slight depletion of lactose, and no significant variation in moisture content or texture; conversely, unpackaged whey cheeses exhibited substantial loss of water and a concomitant increase in rigidity. Vacuum packaging strongly inhibited lipolysis (even if viable counts of some microbial groups were high); saturated fatty acids (mainly C16:0 and C14:0) accounted for ca. 73% of the total free–fatty acid content, whereas the most concentrated unsaturated fatty acids were C18:1 and C18:2 (ca. 14% each). The conclusions generated in our study are, in general, useful for a wide range of whey cheeses worldwide: i.e., Requéson (Spain), Ricotta (Italy), Broccio (France), and Anthotyro (Greece). In addition, our conclusions are particularly helpful in terms of improving the safety of Requeijão, a widely acclaimed dairy specialty.


2011 ◽  
Vol 236-238 ◽  
pp. 2705-2708
Author(s):  
Yan Ping Zhang ◽  
Hui Yun Zhou ◽  
Ling Juan Jiang ◽  
Zhong Dong Wang

This study was carried out to find material basis of which effect germination rate ofSalvia miltiorrhizaBge seeds in different years and in different areas. The results can be used in quality identification and advising storage conditions. Routine method were used to study the basic qualities, such as physical purity, weight of 100 seeds, seed moisture and germination. GC-MS were used to detect fatty acids and UV spectrophotometer was used to detect reducing sugar. The germination and viability of new seeds were much higher than that of aged. Weight of 100 seeds, seed moisture, fatty acids of same origin new seeds were higher than that of aged. However there was no correlation between different areas. Content of reducing sugar of same origin new seeds were higher than that of aged, also there were no correlation between different areas. Unsaturated fatty acids content of the new seeds was 80.90% which was much higher than that of aged. Linoleic acid was the main Unsaturated fatty acids. Saturated fatty acid content was 19.10% which was much lower than that of aged. Palmitic acid was the main saturated fatty acids. Data showed that unsaturated acids were auto oxidized to saturated acids in the process of storage. Germination rate was decreased as storage time prolonged.


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