scholarly journals Ідентифікація небезпечних чинників під час вирощування райдужної форелі в умовах замкнутого водопостачання

2017 ◽  
Vol 19 (78) ◽  
pp. 48-52
Author(s):  
N. Grynevych ◽  
T. Dyman ◽  
M. Kukhtyn ◽  
V. Semanyuk ◽  
A. Sliusarenko
Keyword(s):  
Rainbow Trout ◽  
Larval Survival ◽  
Fish Feeding ◽  
Commercial Fish ◽  
E Coli ◽  
Aquaculture System ◽  
Microbial Number ◽  
Undigested Food ◽  
Aerobic And Anaerobic ◽  
Purification Of Water

Identification of dangerous factors for a conditional industrial farm for rainbow trout growing in conditions of Recurculating aquaculture system (RAS) was carried out. During the analysis of dangerous factors, the degree of significant influence and probability of potential dangers were determined. Significant potential hazards have been identified at such technological stages as insemination and incubation of caviar, larval survival, caviar, larvae, young, commodity fish and pedigree.Biological filter is defined as a key link in the RAS the functioning of which effects on the quality and safety of commercial fish. It carries out aerobic and anaerobic processes to remove contaminations in the form of ammonium producing by fish, and carbon dioxide, which is formed from undigested food and feces. There are such dangerous factors on the stage of biological purification of water: biological – possible exceeding the total microbial number (more than 100 CFU/cm3), the number of nitrifying (more than 106 CFU/cm3) and E. coli groupbacteria, the presence of bacteria Pseudomonas aeruginosa; chemical – exceeding the content of nitrates (more than 50 mg / dm3), nitrites (more than 0.5 mg/dm3), pH – above 8.In addition to purely technological, there are additional dangerous factors – related to the functioning of the biofilter, fish feeding and the use of veterinary preparations and disinfectants. The potential hazards identified in the work are important to be taken into account in the process of implementation of Rainbow Trout HACCP plan. 

Microbiota of the aquatic environment and rainbow trout when grown in the RAS

2021 ◽  
pp. 68-79
Author(s):  
Firdauz Mubarakovna Shakirova ◽  
Lada Konstantinovna Govorkova ◽  
Olga Konstantinovna Anokhina ◽  
Guzel Damirovna Valieva
Keyword(s):  
Rainbow Trout ◽  
Aquatic Environment ◽  
Intestinal Microflora ◽  
Fish Farming ◽  
Microbial Count ◽  
Qualitative Composition ◽  
E Coli ◽  
Microbial Number ◽  
Closed Water ◽  
Sanitary State

Fish production in closed systems is an intensive way of fish farming. However, keeping fish in plants with a closed water supply does not solve the problem of diseases in aquaculture facilities. The greatest damage is caused by bacterial diseases. In this regard, the study of the sanitary state of the aquatic environment of fish is relevant. The article provides information on the physicochemical parameters of water, the species composition of the microflora of the aquatic environment in the basin, microbiological indicators of the sanitary state of water, the total microbial count (TMC) and the number of bacteria of the E. coli group (BCGC) in the RAS when growing rainbow trout. Sanitary indicators of water quality in the pool, both in the first days of keeping rainbow trout, and after a week were within the normal range. There was only a numerical increase in the total microbial number and bacteria of the E. coli group in water samples taken after a week of finding the trout in the pools. According to the results of a study of the contamination of the skin and gills of rainbow trout, as well as the water in which it is contained, an increase in time was revealed from 69 to 73 CFU/ml on the skin and from 85 to 89 CFU/ml on the gills within a week. The qualitative composition of the microflora of the skin and gills of rainbow trout is represented by bacteria of the genera Aeromonas, Pseudomonas, Bacillus, Enterobacter, which is similar to the qualitative composition of the microflora of water. The study of the intestinal microflora of rainbow trout revealed that it also contains bacteria of the genera Aeromonas, Pseudomonas, Bacillus, Enterobacter. At the same time, lactic acid, streptococcal, staphylococcal bacteria, yeast-like fungi of the genus Candida were found, which are normal representatives of the intestinal microflora in small quantities.

scholarly journals In Vitro and In Vivo Antibacterial Activities of a Novel Glycylcycline, the 9-t-Butylglycylamido Derivative of Minocycline (GAR-936)

1999 ◽  
Vol 43 (4) ◽  
pp. 738-744 ◽  
Author(s):  
P. J. Petersen ◽  
N. V. Jacobus ◽  
W. J. Weiss ◽  
P. E. Sum ◽  
R. T. Testa
Keyword(s):  
Anaerobic Bacteria ◽  
Tetracycline Resistance ◽  
Protective Effects ◽  
Gram Negative ◽  
E Coli ◽  
Resistance Determinants ◽  
Aerobic And Anaerobic ◽  
Ribosomal Protection

ABSTRACT The 9-t-butylglycylamido derivative of minocycline (TBG-MINO) is a recently synthesized member of a novel group of antibiotics, the glycylcyclines. This new derivative, like the first glycylcyclines, theN,N-dimethylglycylamido derivative of minocycline and 6-demethyl-6-deoxytetracycline, possesses activity against bacterial isolates containing the two major determinants responsible for tetracycline resistance: ribosomal protection and active efflux. The in vitro activities of TBG-MINO and the comparative agents were evaluated against strains with characterized tetracycline resistance as well as a spectrum of recent clinical aerobic and anaerobic gram-positive and gram-negative bacteria. TBG-MINO, with an MIC range of 0.25 to 0.5 μg/ml, showed good activity against strains expressing tet(M) (ribosomal protection), tet(A), tet(B),tet(C), tet(D), and tet(K) (efflux resistance determinants). TBG-MINO exhibited similar activity against methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant streptococci, and vancomycin-resistant enterococci (MICs at which 90% of strains are inhibited, ≤0.5 μg/ml). TBG-MINO exhibited activity against a wide diversity of gram-negative aerobic and anaerobic bacteria, most of which were less susceptible to tetracycline and minocycline. The in vivo protective effects of TBG-MINO were examined against acute lethal infections in mice caused by Escherichia coli, S. aureus, andStreptococcus pneumoniae isolates. TBG-MINO, administered intravenously, demonstrated efficacy against infections caused byS. aureus including MRSA strains and strains containingtet(K) or tet(M) resistance determinants (median effective doses [ED50s], 0.79 to 2.3 mg/kg of body weight). TBG-MINO demonstrated efficacy against infections caused by tetracycline-sensitive E. coli strains as well asE. coli strains containing either tet(M) or the efflux determinant tet(A), tet(B), ortet(C) (ED50s, 1.5 to 3.5 mg/kg). Overall, TBG-MINO shows antibacterial activity against a wide spectrum of gram-positive and gram-negative aerobic and anaerobic bacteria including strains resistant to other chemotherapeutic agents. The in vivo protective effects, especially against infections caused by resistant bacteria, corresponded with the in vitro activity of TBG-MINO.

Welfare assessment of rainbow trout reared in a Recirculating Aquaculture System: Comparison with a Flow-Through System

Aquaculture ◽  
2015 ◽  
Vol 436 ◽  
pp. 151-159 ◽  
Author(s):  
Violaine Colson ◽  
Bastien Sadoul ◽  
Claudiane Valotaire ◽  
Patrick Prunet ◽  
Matthieu Gaumé ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Recirculating Aquaculture System ◽  
Welfare Assessment ◽  
Recirculating Aquaculture ◽  
Aquaculture System ◽  
Flow Through

A Bacteriological Survey of Raw Ground Beef1

1977 ◽  
Vol 40 (11) ◽  
pp. 790-794 ◽  
Author(s):  
JAMES F. FOSTER ◽  
JAMES L. FOWLER ◽  
WARREN C. LADIGES
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Retail Store ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Fecal Streptococci ◽  
Plate Counts ◽  
Aerobic And Anaerobic

The microbiological quality of 150 units of raw ground beef obtained from a local retail store was determined. The range of aerobic plate counts was from 6.9 × 104 to 8.3 × 107/g. By using the most probable number method 96.7% of the 150 units were positive for coliforms, 94.7% for Escherichia coli and 61.3% for Staphylococcus aureus. By the plate methods, 99.3% of the units were positive for fecal streptococci and 56% were positive for Clostridium perfringens. No salmonellae were isolated. Aerobic and anaerobic organisms were isolated and identified. E. coli was the most frequently isolated aerobe followed by organisms in the Klebsiella-Enterobacter group. Among the anaerobic isolates, C. perfringens was the organism most frequently encountered.

Bacterial Counts of Commercial Fish Diets

1971 ◽  
Vol 28 (8) ◽  
pp. 1185-1189 ◽  
Author(s):  
T. J. Trust
Keyword(s):  
Fluorescent Pseudomonads ◽  
Total Count ◽  
Aerobic Bacteria ◽  
Fish Production ◽  
Commercial Fish ◽  
Fish Diets ◽  
Thermophilic Species ◽  
Aerobic And Anaerobic ◽  
Spore Forming Bacteria ◽  
Representative Samples

Two diets formulated and offered for commercial fish production were examined to determine their microbial burden. The total count of aerobic bacteria ranged from 103 to 107 bacteria per gram of diet. The diets contained psychrophilic and thermophilic species and up to 104 aerobic and anaerobic spore-forming bacteria per gram. Enterococci and members of the Enterobacteriaceae, including species of Salmonella, were also present. Fluorescent pseudomonads were detected in 18 of the 47 samples tested, Proteolytic, amylolytic, lipolytic, and haemolytic organisms were shown to be present in measurable numbers. The total viable aerobic count did not change markedly on storage of representative samples at 4, 20, or 30 C.

scholarly journals TmSpz4 Plays an Important Role in Regulating the Production of Antimicrobial Peptides in Response to Escherichia coli and Candida albicans Infections

2020 ◽  
Vol 21 (5) ◽  
pp. 1878 ◽  
Author(s):  
Tariku Tesfaye Edosa ◽  
Yong Hun Jo ◽  
Maryam Keshavarz ◽  
Young Min Bae ◽  
Dong Hyun Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Immune Responses ◽  
Fungal Infections ◽  
Larval Survival ◽  
Gram Negative Bacteria ◽  
Functional Importance ◽  
E Coli ◽  
Microbial Infections

Spätzle family proteins activate the Toll pathway and induce antimicrobial peptide (AMP) production against microbial infections. However, the functional importance of Tmspätzle4 (TmSpz4) in the immune response of Tenebrio molitor has not been reported. Therefore, here, we have identified and functionally characterized the role of TmSpz4 against bacterial and fungal infections. We showed that TmSpz4 expression was significantly induced in hemocytes at 6 h post-injection with Escherichia coli, Staphylococcus aureus, and Candida albicans. TmSpz4 knock-down significantly reduced larval survival against E. coli and C. albicans. To understand the reason for the survivability difference, the role of TmSpz4 in AMP production was examined in TmSpz4-silenced larvae following microbe injection. The AMPs that are active against Gram-negative bacteria, including TmTenecin-2, TmTenecin-4, TmAttacin-1a, TmDefensin-2, and TmCecropin-2, were significantly downregulated in response to E. coli in TmSpz4-silenced larvae. Similarly, the expression of TmTenecin-1, TmTenecin-3, TmThaumatin-like protein-1 and -2, TmDefensin-1, TmDefensin-2, and TmCecropin-2 were downregulated in response to C. albicans in TmSpz4-silenced larvae. In addition, the transcription factor NF-κB (TmDorX1 and TmDorX2) expression was significantly suppression in TmSpz4-silenced larvae. In conclusion, these results suggest that TmSpz4 plays a key role in regulating immune responses of T. molitor against to E. coli and C. albicans.

scholarly journals Features of rainbow trout farming technology in PE “Zakhidna Rybna Kompania”, for feeding with Aguafeed Fischfutteer

2020 ◽  
Vol 22 (93) ◽  
pp. 142-147
Author(s):  
V. I. Bozhyk ◽  
O. V. Bozhyk
Keyword(s):  
Rainbow Trout ◽  
Water Supply ◽  
Objective Assessment ◽  
High Energy ◽  
High Protein ◽  
Fish Growth ◽  
Feed Ratio ◽  
General Description ◽  
Commercial Fish

The production of trout products in ponds and aquaculture farms is based on modern and long-term plans for technology and intensification of production, through the introduction of the latest approaches, by borrowing the experience of leading foreign producers, expanding production areas and sectoral investments. The main indicator that characterizes the efficiency of fisheries and reflects the state of production and the level of its economic indicators. is fish productivity Growing trout in aquaculture in a closed water supply is associated with high planting densities per unit area, the use of high-protein granular feed, which must fully meet the needs of fish in all necessary nutrients to ensure a balanced diet. In modern farms for feeding trout use high-protein feed mixtures in the form of grits and pellets. The dynamics of the intensity of growth and development of rainbow trout mainly depends on the location of the farm, the quality of the water supply source, the feeding regime of fish and the quality of feed. Therefore, the aim of the work was based on a review of literature sources and analysis of economic activity of the studied farm to give a general description of trout feed, Aquafeed Fischfutter, used for fish feeding, and determine their effectiveness, taking into account geographical location and abiotic and biotic factors . In addition, the study fish performance when feeding these feeds. Conduct research on the growth rate of rainbow trout, changes in weight and length of larvae, fry, yearlings and commercial fish, in tray, pool and pond cultivation. Develop a scheme for growing all age groups of trout on these feeds. Establish indicators of quality, nutritional value, feed ratio of starting, growth and production feeds. To give an objective assessment of their impact on the growth of larvae, fry, yearlings and commercial rainbow trout in the conditions of trout farming PE “Zakhidna Rybna Kompania”, Perimyshlyany district, Lviv region. The conducted research gives the basis to claim that this compound feed guarantees high daily gain. Being a high-energy feed, its use does not lead to obesity of fish. With intensive feeding, a good ratio of price and productivity of farmed fish is realized. The risk of contamination of water bodies and fish is very low, due to the low phosphorus content and good digestibility of feed – 1.1 kg of feed/kg of fish growth. The feed corresponds to the best world indicators in accordance with buoyancy and stability in water.

scholarly journals Growth of cell wall-defective variants of Escherichia coli: comparison of aerobic and anaerobic induction frequencies

1977 ◽  
Vol 6 (2) ◽  
pp. 166-171
Author(s):  
T W Huber ◽  
A W Brinkley
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
High Frequency ◽  
Anaerobic Incubation ◽  
Anaerobic Conditions ◽  
Group Iii ◽  
E Coli ◽  
Induction Frequency ◽  
Group I ◽  
Aerobic And Anaerobic

A method for quantitating the conversion of Escherichia coli to colony-forming, cell wall-defective (CWD) bacteria has been developed. The induction frequency, i.e., the percentage of the population recovered as CWD colonies was determined for 20 randomly selected clinical isolates of E. coli under aerobic and anaerobic incubation conditions. Penicillin (1,000 U/ML) was the inducing agent. The 20 strains segregated into three groups. Group I organisms produced CWD colonies with high frequency both aerobically and anaerobically. Grout II organisms showed a much higher induction frequency anaerobically than aerobically. Group III organisms were poor inducers. Thirty percent of the strains were group I, 50% were group II, and 20% were group III organisms. These data indicate that anaerobic conditions enhance the induction and growth of CWD E. coli in the research laboratory and suggest that anaerobic incubation may be important in recovery of medically significant CWD bacteria.

scholarly journals Evaluation of Growth Performance of a Genetically Characterized F1 Rainbow Trout, <i>Oncorhynchus mykiss</i> in a Recirculating Aquaculture System

2017 ◽  
Vol 08 (07) ◽  
pp. 716-728
Author(s):  
José Luis Arredondo-Figueroa ◽  
Nayeli Ismene Armendáriz-Sáenz ◽  
Laura Georgina Núñez-García ◽  
Jesús Trinidad Ponce-Palafox ◽  
Irene De Los Angeles Barriga-Sosa
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Growth Performance ◽  
Recirculating Aquaculture System ◽  
Recirculating Aquaculture ◽  
Aquaculture System

scholarly journals PRACTICE OF ARTIFICIAL REPRODUCTION OF STERLAD (ACIPENSER RUTHENUS LINNAEUS) IN RECIRCULATING AQUACULTURE SYSTEM (IN MANGISTAU REGION)

2020 ◽  
Vol 2020 (3) ◽  
pp. 111-124
Author(s):  
Yefim Fedorovich Bulavin ◽  
Guldana Maratkizi Maratova ◽  
Saule Zhangirovna Asylbekova ◽  
Kuanysh Baibulatovich Isbekov ◽  
Saya Kashkinbayevna Koyshibaeva ◽  
...  
Keyword(s):  
Fish Farming ◽  
Artificial Reproduction ◽  
Recirculating Aquaculture System ◽  
Hormonal Stimulation ◽  
Commercial Fish ◽  
Acipenser Ruthenus ◽  
Recirculating Aquaculture ◽  
Aquaculture System ◽  
Sturgeon Species ◽  
Sterlet Acipenser Ruthenus

The paper presents the results of artificial reproduction of sterlet (Acipenser ruthenus Linnaeus) carried out at the Scientific Production Center KazakhOsseter, LLP (the Mangistau region) in conditions of a recirculating aquatic system (RAS). The conditions for carrying out the main reproduction measures are described: wintering, qualifying, hormonal stimulation, obtaining reproductive products and rearing larvae. As a result, the sterlet spawning campaign was carried out in conditions of RAS in the Mangistau region, the native grown viable sterlet larvae being obtained. In the course of the work carried out in conditions of RAS in the Mangistau region there have been developed the scientific foundations and technologies for the formation and using production stocks of sturgeon, which are involved in the process of artificial reproduction. The results of the work performed could help to effectively organize the production at the commercial fish farming enterprises in the Mangistau region. The entrepreneurs of the region will have the opportunity to get acquainted with the technology of fish growing in conditions of RAS and to apply the technology for obtaining and reproducing sturgeon species in the ir local hatcheries in the future.

Sign in / Sign up

Export Citation Format

Share Document