Microbiota of the aquatic environment and rainbow trout when grown in the RAS

2021 ◽  
pp. 68-79
Author(s):  
Firdauz Mubarakovna Shakirova ◽  
Lada Konstantinovna Govorkova ◽  
Olga Konstantinovna Anokhina ◽  
Guzel Damirovna Valieva
Keyword(s):  
Rainbow Trout ◽  
Aquatic Environment ◽  
Intestinal Microflora ◽  
Fish Farming ◽  
Microbial Count ◽  
Qualitative Composition ◽  
E Coli ◽  
Microbial Number ◽  
Closed Water ◽  
Sanitary State

Fish production in closed systems is an intensive way of fish farming. However, keeping fish in plants with a closed water supply does not solve the problem of diseases in aquaculture facilities. The greatest damage is caused by bacterial diseases. In this regard, the study of the sanitary state of the aquatic environment of fish is relevant. The article provides information on the physicochemical parameters of water, the species composition of the microflora of the aquatic environment in the basin, microbiological indicators of the sanitary state of water, the total microbial count (TMC) and the number of bacteria of the E. coli group (BCGC) in the RAS when growing rainbow trout. Sanitary indicators of water quality in the pool, both in the first days of keeping rainbow trout, and after a week were within the normal range. There was only a numerical increase in the total microbial number and bacteria of the E. coli group in water samples taken after a week of finding the trout in the pools. According to the results of a study of the contamination of the skin and gills of rainbow trout, as well as the water in which it is contained, an increase in time was revealed from 69 to 73 CFU/ml on the skin and from 85 to 89 CFU/ml on the gills within a week. The qualitative composition of the microflora of the skin and gills of rainbow trout is represented by bacteria of the genera Aeromonas, Pseudomonas, Bacillus, Enterobacter, which is similar to the qualitative composition of the microflora of water. The study of the intestinal microflora of rainbow trout revealed that it also contains bacteria of the genera Aeromonas, Pseudomonas, Bacillus, Enterobacter. At the same time, lactic acid, streptococcal, staphylococcal bacteria, yeast-like fungi of the genus Candida were found, which are normal representatives of the intestinal microflora in small quantities.

scholarly journals Ідентифікація небезпечних чинників під час вирощування райдужної форелі в умовах замкнутого водопостачання

2017 ◽  
Vol 19 (78) ◽  
pp. 48-52
Author(s):  
N. Grynevych ◽  
T. Dyman ◽  
M. Kukhtyn ◽  
V. Semanyuk ◽  
A. Sliusarenko
Keyword(s):  
Rainbow Trout ◽  
Larval Survival ◽  
Fish Feeding ◽  
Commercial Fish ◽  
E Coli ◽  
Aquaculture System ◽  
Microbial Number ◽  
Undigested Food ◽  
Aerobic And Anaerobic ◽  
Purification Of Water

Identification of dangerous factors for a conditional industrial farm for rainbow trout growing in conditions of Recurculating aquaculture system (RAS) was carried out. During the analysis of dangerous factors, the degree of significant influence and probability of potential dangers were determined. Significant potential hazards have been identified at such technological stages as insemination and incubation of caviar, larval survival, caviar, larvae, young, commodity fish and pedigree.Biological filter is defined as a key link in the RAS the functioning of which effects on the quality and safety of commercial fish. It carries out aerobic and anaerobic processes to remove contaminations in the form of ammonium producing by fish, and carbon dioxide, which is formed from undigested food and feces. There are such dangerous factors on the stage of biological purification of water: biological – possible exceeding the total microbial number (more than 100 CFU/cm3), the number of nitrifying (more than 106 CFU/cm3) and E. coli groupbacteria, the presence of bacteria Pseudomonas aeruginosa; chemical – exceeding the content of nitrates (more than 50 mg / dm3), nitrites (more than 0.5 mg/dm3), pH – above 8.In addition to purely technological, there are additional dangerous factors – related to the functioning of the biofilter, fish feeding and the use of veterinary preparations and disinfectants. The potential hazards identified in the work are important to be taken into account in the process of implementation of Rainbow Trout HACCP plan. 

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

Fish farming in Denmark: environmental impact of regulative legislation

1995 ◽  
Vol 31 (10) ◽  
pp. 73-84 ◽  
Author(s):  
T. M. Iversen
Keyword(s):  
Organic Matter ◽  
Environmental Impact ◽  
Freshwater Fish ◽  
Aquatic Environment ◽  
Action Plan ◽  
Fish Farming ◽  
Fish Farms ◽  
Nitrogen And Phosphorus ◽  
Local Impact ◽  
The Impact

The main environmental problems associated with fish farming in Denmark are attributable to the dam, the “dead reach” and nutrient and organic matter discharge. The environmental regulation of fish farming in Denmark started with the Environmental Protection Act of 1974, the Statutory Order of 1985 forbidding wet feed, and the Action Plan on the Aquatic Environment of 1987. In the case of freshwater fish farms, the latter was implemented through the measures stipulated in the 1989 Statutory Order on Fish Farms. The impact of Danish legislative measures to reduce and regulate the environmental effects of freshwater fish farms can be summarized as follows: - the number of fish farms has been reduced from about 800 in 1974 to about 500 at present; - production has tripled since 1974 and has been stable since 1989; - a change from wet to dry feed has reduced the environmental impact of the farms; - the national goals of the Action Plan on the Aquatic Environment of 1987 for reducing fish farm discharges of organic matter, nitrogen and phosphorus have been fulfilled. The main remaining problems are that: - the local impact of fish farms on downstream stream quality is still much too high in about 15% of cases; - the problem of the passage of migrating invertebrates and fish is still unsolved at some farms; - the problems posed by “dead reaches” are still unsolved. It is concluded that sustainable fish farming is possible in Denmark, but with the present technology production will have to be significantly reduced.

scholarly journals Investigation of the Physical, Chemical and Microbiological Stability of Losartan Potassium 5 mg/mL Extemporaneous Oral Liquid Suspension

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 301
Author(s):  
Lisa Foley ◽  
Jennifer Toney ◽  
James W. Barlow ◽  
Maura O’Connor ◽  
Deirdre Fitzgerald-Hughes ◽  
...  
Keyword(s):  
Shelf Life ◽  
Storage Conditions ◽  
Losartan Potassium ◽  
Microbial Count ◽  
E Coli ◽  
Physical Chemical ◽  
Liquid Suspension ◽  
Oral Liquid ◽  
Microbiological Stability ◽  
Stability Data

Extemporaneous oral liquid preparations are commonly used when there is no commercially available dosage form for adjustable dosing. In most cases, there is a lack of stability data to allow for an accurately assigned shelf life and storage conditions to give greater confidence of product safety and efficacy over its shelf life. The aim of this study was to evaluate the physical, chemical and microbiological stability of an extemporaneous oral liquid suspension of losartan potassium, 5 mg/mL, used to treat paediatric hypertension in Our Lady’s Children’s Hospital Crumlin, Ireland. The losartan content of extemporaneous oral suspensions, prepared with and without addition of water, was measured by UV and confirmed by HPLC analysis. Suspensions were stored at 4 °C and room temperature (RT) and were monitored for changes in; pH, colour, odour, re-dispersibility, Total Aerobic Microbial Count, Total Yeast and Mould Count and absence of E. coli. Results showed that suspensions prepared by both methods, stored at 4 °C and RT, were physically and microbiologically stable over 28 days. Initial losartan content of all suspensions was lower than expected at 80–81% and did not change significantly over the 28 days. HPLC and NMR did not detect degradation of losartan in the samples. Suspensions prepared in water showed 100% losartan content. The reduced initial losartan content was confirmed by HPLC and was related to the acidic pH of the suspension vehicle. Physiochemical properties of the drug are important factors for consideration in the selection of suspension vehicle for extemporaneous compounding of oral suspensions as they can influence the quality, homogeneity and efficacy of these preparations.

scholarly journals Quantifying within- and between-animal variation and uncertainty associated with counts of Escherichia coli O157 occurring in naturally infected cattle faeces

2008 ◽  
Vol 6 (31) ◽  
pp. 169-177 ◽  
Author(s):  
S.E Robinson ◽  
P.E Brown ◽  
E.J Wright ◽  
C.A Hart ◽  
N.P French
Keyword(s):  
Escherichia Coli ◽  
Statistical Model ◽  
Human Infection ◽  
Microbial Count ◽  
Infected Cattle ◽  
Explanatory Variables ◽  
E Coli ◽  
Fixed And Random Effects ◽  
Cattle Faeces ◽  
Multiple Samples

Cattle faeces are considered the most important reservoir for human infection with Escherichia coli O157. We have previously described shedding of E. coli O157 in the faeces of naturally infected cattle cohorts. However, the data require further investigation to quantify the uncertainty and variability in the estimates previously presented. This paper proposes a method for analysing both the presence and the quantity of E. coli O157 in cattle faecal samples, using two isolation procedures, one of which enumerates E. coli O157. The combination of these two measurements, which are fundamentally different in nature and yet measuring a common outcome, has necessitated the development of a novel statistical model for ascertaining the contribution of the various components of variation (both natural and observation induced) and for judging the influence of explanatory variables. Most of the variation within the sampling hierarchy was attributable to multiple samples from the same animal. The contribution of laboratory-level variation was found to be low. After adjusting for fixed and random effects, short periods of increased intensity of shedding were identified in individual animals. We conclude that within-animal variation is greater than between animals over time, and studies aiming to elucidate the dynamics of shedding should focus resources, sampling more within than between animals. These findings have implications for the identification of persistent high shedders and for assessing their role in the epidemiology of E. coli O157 in cattle populations. The development of this non-standard statistical model may have many applications to other microbial count data.

Growing commodity tilapia in various experimental conditions

2020 ◽  
pp. 26-36
Author(s):  
F. A. Altayeva
Keyword(s):  
Fish Farming ◽  
Biological Indicators ◽  
Fish Farms ◽  
Experimental Conditions ◽  
Fish Breeding ◽  
Closed Water ◽  
The Republic ◽  
Short Time ◽  
High Fish ◽  
Experimental Ponds

The article presents the results of growing tilapia to marketable weight in experimental sites (Chilikskoye pond pond farms LLP, KATU named after S. Seifullin JSC and Kapshagai spawning farm — 1973 LLP). In the process of carrying out research works, the methods generally accepted in fish farming, hydrochemistry, hydrobiology, hydrology, ichthyology, as well as foreign normative and technical guidelines and methodological instructions on the technology of growing commercial tilapia in industrial conditions were used. Monitoring of hydrochemical analyses of water in ponds, swimming pools, and closed water supply installations is given. Fish-breeding and biological indicators of tilapia grown in various experimental conditions are presented. The results of the studies have shown the real possibility of growing tilapia in various conditions of fish farms in Kazakhstan. Based on the results of the work, the following conclusions were made: — satisfactory fish-breeding indicators were obtained with various cultivation technologies (as when growing tilapia in polyculture and monoculture in ponds, in pools, as well as cultivation in the recirculation system); — the cultivation of tilapia in the experimental ponds of the fish farm of the Almaty region of the Republic of Kazakhstan has shown the possibility of obtaining high fish-breeding biological indicators of tilapia and the ability to grow to marketable mass in a short time; — as a result of experimental cultivation in various conditions, it was found that the survival rate was not lower than 97%; — as a result of the analysis of the profitability indices of the developed technologies, it was found that the most profitable was the technology of growing marketable tilapia products in monoculture ponds on water with a natural thermal regime in Chilik Pond Economy LLP.

Correlation Between Intestinal Microflora and Gastrointestinal Dysfunction in Elderly Patients with Sepsis

2020 ◽  
Vol 12 (8) ◽  
pp. 1030-1037
Author(s):  
Hailing Yang ◽  
Xiaolin Zhang ◽  
Weijun Chen ◽  
Libo Shang ◽  
Bin Chen ◽  
...  
Keyword(s):  
Elderly Patients ◽  
Intestinal Microflora ◽  
Sepsis Group ◽  
Control Group ◽  
Gastrointestinal Dysfunction ◽  
E Coli ◽  
Significant Difference ◽  
Prevention And Treatment ◽  
The Common ◽  
Wbc Count

Multiple organ dysfunction syndrome (MODS) is a key factor that leads to death in elderly patients with sepsis. Therefore, early prevention and treatment of gastrointestinal dysfunction (GIDF) in elderly patients with sepsis is an important measure to prevent MODS occurrence. This research explores the correlation between intestinal microflora and GIDF in elderly patients with sepsis and provides ideas for the prevention and treatment of GIDF in elderly patients with sepsis. In this study, 152 patients with sepsis (122 patients with sepsis and GIDF) treated in the Third Affiliated Hospital of Yunnan University of Chinese Medicine from January to September 2019 were selected as the sepsis group and 100 elderly who had normal physical examination results were selected as the control group. The common intestinal microflora of the two groups was compared. Patients with sepsis and GIDF were treated as the GIDF group and the other patients with sepsis were treated as the non-GIDF group. The common intestinal microflora, gastrointestinal indicators, serum inflammatory factors, and immune function indices were compared between the two groups. Correlation analysis of the observed indices with statistical significance was carried out. The results showed 152 patients with sepsis and 122 patients with sepsis and GIDF; thus, the incidence of sepsis with GIDF was 80.26%. The total average score of sepsis with GIDF was 3.61±0.09. There was no statistically significant difference in GIDF scores of patients ages 65–75 and > 75 years old. The number of Bifidobacterium and Lactobacillus in elderly patients with sepsis was lower and the number of Escherichia coli was higher than in the control group. In elderly patients with sepsis, the number of Bifidobacterium and Lactobacillus in the GIDF group was lower and the number of E. coli was higher than in the non-GIDF group. White blood cell (WBC) count, procalcitonin (PCT), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), gastrin (GAS), and diamine oxidase (DAO) in GIDF patients were higher and motilin (MOT), CIT (CIT), CD4+, and CD8+ were lower than in the non-GIDF group. WBC count, PCT, CRP, TNF-α, GAS, and DAO were negatively correlated with the number of Bifidobacterium and Lactobacillus but positively correlated with E. coli. MOT, CIT, CD4+, and CD8+ were positively correlated with the number of Bifidobacterium and Lactobacillus but negatively correlated with E. coli. There was a negative correlation between Bifidobacterium and Lactobacillus and GIDF score and a positive correlation between E. coli and GIDF score. Therefore, the change in the intestinal microflora in elderly patients with sepsis is related to GIDF.

Factors Contributing to the Persistence of E. coli in the Human Large Intestinal Microflora

1991 ◽  
pp. 331-333
Author(s):  
Agnes E. Wold ◽  
Dominique Caugant ◽  
Gunilla Lidin-Jansson ◽  
Peter de Man ◽  
Catharina Svanborg
Keyword(s):  
Intestinal Microflora ◽  
E Coli

scholarly journals Denitrifying bacteria in the limnetic zone of Lake Tota, Colombia

2021 ◽  
Vol 26 (1) ◽  
pp. 1-16
Author(s):  
Julian Esteban Másmela-Mendoza ◽  
Luz Marina Lizarazo Forero
Keyword(s):  
Rainbow Trout ◽  
Fish Farming ◽  
Sampling Period ◽  
Denitrifying Bacteria ◽  
Most Probable Number ◽  
Nitrogen And Phosphorus ◽  
Probable Number ◽  
Physicochemical Factors ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria

The objective of study was to isolate and determine the identity of denitrifying bacteria from limnetic areas of Lake Tota (Colombian Andes) with and without rainbow trout production activities. We examined the relationships between the lake’s physicochemical factors (oxygen, nitrogen, and phosphorus content) and two bacterial communities (denitrifying bacteria and coliforms). Water samples were taken 20m below the surface from July to September at five limnetic zones; two of which were close to rainbow trout farming areas. In each zone, the concentrations of oxygen, nitrogen, and phosphorus were measured. To identify and quantify the abundance of bacteria, the most probable number (MPN) technique was used, employing minimal medium for denitrifying bacteria and medium for nitrate reducing bacteria (NRB). A greater number of denitrifying bacteria were found in the fish farming zones, identifying bacteria of the genera Bacillus, Pseudomonas, Nocardia, and Streptomyces. The number of nitrate-reducing bacteria revealed statistically significant differences throughout the sampling period, increasing from July to September and was related to a decrease in precipitation. The density of NRB and total phosphorus were directly correlated. High bacterial densities of denitrifyingbacteria and coliforms are indicative of changes from oligotrophic to eutrophic states in the studied limnetic areas.

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