Relación entre las características de los transportes, con las características nutricionales de la carne porcina destinada a consumo humano en el Valle de Aburrá, 2017

Pork meat is considered a source of high nutritional value due to its high protein content, however, transport is a critical link to generate economic losses by producing alterations in animal welfare, which have an impact on nutritional parameters, decreasing the capacity of water retention, and generating protein losses. The objective of this study was to relate the characteristics of transport, with the nutritional characteristics of pork for human consumption in the Valle de Aburrá in 2017. Three slaughterhouses of Valle de Aburrá were visited with probabilistic sampling, stratified by plant and equal affixation, obtaining information from 338 animals. The nutritional parameters of the meat samples and sociodemographic variables, infrastructure, animal welfare and driving practices in the transporters were analyzed. Chi square tests were performed for dichotomous qualitative variables, logistic regression for qualitative polytomous variables and U Mann - Whitney for quantitative variables. An association was found between several of the parameters investigated with statistically significant p values (p = 0.000), where, having no permanent water supply for the animals increases the possibility of presenting nutritionally inadequate meats 46.55 times (IC 18.08 - 120.07). It concludes that factors such as lack of water supply to pigs, poor condition of the floors and separators, lack of training in transporters, lack of supervision of animals, lack of mechanical technical certification and average speed of 80 Km/Hr are associated with the generation of nutritionally inadequate meat products.

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Molecular characterization of Toxoplasma gondii and Sarcocystis spp. in raw kibbeh and other meat samples commercialized in Botucatu, Southeastern Brazil

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612021051 ◽

2021 ◽

Vol 30 (2) ◽

Author(s):

Helio Langoni ◽

Diego Generoso ◽

Ênio Yoshinori Hayasaka ◽

Karine Bott Mantovan ◽

Benedito Donizete Menozzi ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Meat Products ◽

Positive Sample ◽

Southeastern Brazil ◽

Economic Losses ◽

Base Pairs ◽

Sarcocystis Spp ◽

Commercial Markets ◽

Meat Samples ◽

Pcr Targeting

Abstract Toxoplasmosis occurs worldwide causing economic losses to the animal production and problems to the public health. The study aimed to detect Toxoplasma gondii and Sarcocystis spp.in 141 meat products from commercial meat cuts of pork, beef, and kibbeh sold in commercial markets from Botucatu, SP, Brazil. Samples were bioassayed in mice to isolate the parasite, and the parasite DNA detected by PCR targeting the 529 base pairs repeat element region (PCR-529-bp). All samples resulted negative on bioassay, whereas PCR positive for 9 (6,38%), distributed as 5/48 beef, 3/49 pork, and 1/44 kibbeh. PCR-positive were investigated for the the parasite genotype using multiplex-, nested-, and RFLP-PCR for 11 markers (SAG1, 5’-3’SAG2, alt.SAG2, SAG3, B-TUB, GRA6, L358, c22-8, c29-6, PK1, Apico). Complete genotype was determined on just one PCR-positive sample that matched MAS, TgCkBr89 and TgCkBr147 isolates already identified. In addition, nested- and RFLP-PCR targeting 18S rRNA was run for all PCR-positive samples and, the products, sequenced and aligned to the GenBank at NCBI website. Four samples showed 100% homology with T. gondii (GenBank #L37415.1), three with Sarcocystis hominis (GenBank #AF006471.1), two Sarcocystis cruzi (GenBank #AF176934.1), and one Sarcocystis hirsuta (GenBank #AF006469.1), indicating the circulation of T. gondii and Sarcocystis spp.

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Healthy foods for human consumption produced with amaranth, chia and quinoa seeds and precooked soybean

10.30574/msarr.2021.2.1.0048 ◽

2021 ◽

Vol 2 (1) ◽

pp. 067-073

Author(s):

Hugo Dante Genta ◽

César Augusto Albarracin ◽

Marcela Adriana D’Urso Villar ◽

Cecilia Huerta Macchiarola ◽

Claudia Mónica Brito ◽

...

Keyword(s):

Nutritional Composition ◽

Human Consumption ◽

Healthy Foods ◽

Chi Square ◽

Nutritional Values ◽

Sweet Food ◽

Qualitative Variables ◽

Chi Square Test ◽

Sweet Foods ◽

Age Range

The purpose of this report is to improve and make higher benefits on human diet through the consumption of seeds having high nutritional values. Different combinations of amaranth, chia and quinoa seeds and precooked soybean were used to elaborate a new sweet food. Mixed seeds (flour) and soybean whit peanut, sugar or stevia, glucose, hydrogenated oil and natural essence were prepared and tasted by people of both sex and age range from 1 to 78 years old. Previously nutritional composition was analyzed in the different samples. Sweet foods samples were given to persons to evaluate the acceptance and preference of them compared with two market candies of similar composition. The association analysis was performed using t-test and Analysis of Variance (ANOVA) with Bonferroni´s multiple comparisons for quantitative variables and chi square test for qualitative variables. From all the samples having a standard protein content (more than 10% each), have a higher acceptance those composed by amaranth, chia and quinoa, including more acceptance by women. The same result was obtained respect the preference. The production for human consumption of this new sweet food would imply a better use of vegetable proteins as a complement of the diet animal proteins and improve the health preventive advantages.

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Monitoring of the Listeria spp. identification from the poultry products in the Dnipropetrovsk region

Scientific Messenger of LNU of Veterinary Medicine and Biotechnology ◽

10.32718/nvlvet9318 ◽

2019 ◽

Vol 21 (93) ◽

pp. 103-108

Author(s):

N. M. Zazharska ◽

I. V. Borovuk

Keyword(s):

Meat Products ◽

Food Contamination ◽

Biochemical Properties ◽

Food Plants ◽

Economic Losses ◽

Microbiological Analysis ◽

Normative Documents ◽

Differential Identification ◽

Food Taking ◽

Meat Samples

Due to high mortality, listeriosis is one of the most common causes of death from illnesses associated with food, taking the second place after salmonellosis. Listeriosis, as a rule, arises as a result of consumption of contaminated products, including meat products, cheese, ready-to-eat foods. L. monocytogenes belongs to the third group of pathogenicity. Contamination by L. monocytogenes in processing of products is a constant problem in food plants. Food contamination Listeria leads to a withdrawal of products that produces economic losses. Analysis of the dynamic detection and of the differential identification of Listeria spp. in the meat products of poultry processing enterprises in Dnipropetrovsk region was conducted. The research was carried out by Dnipropetrovsk regional state laboratory of the state service of Ukraine for food safety and consumer protection. The results of bacteriological researches of meat samples which poultry plants gave for microbiological analysis during period 2008–2018 were used for monitoring. Microbiological research was carried out in accordance with valid international normative documents. The fluorescence analyzer Mini Vidas, France, the CAMP test were used for analysis. The biochemical properties of isolated microorganisms were established using BioMerieux API tests, France. Analyzing the number of researches and identification of microorganisms in the Dnipropetrovsk region for the period of 11 years, 3001 positive results out of 8172 analyzed samples were found (36.7%). Herewith, part of positive samples goes up from 8.5% in 2008 to 77.9% in 2018. L. ivanovii was isolated in 1523 samples (18.6%), L. inocua – 833 (10.2%), L. monocytogenes – 493 (6%), L. seeliger – 97 (1.2%), L. grayi – 36 (0.4%), L. welshimeri in 19 samples of meat products (0.2%) out of the 8172 microbiological studies conducted over 11 years. Of the six types of identified Listeria, more than half were L. ivanovii, which is twice as high as cases with L. incocua and thrice compared to L. monocytogenes.

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Trimming and production losses associated with bacterial arthritis in lambs presented to an abattoir in southern Australia

Animal Production Science ◽

10.1071/an17427 ◽

2019 ◽

Vol 59 (5) ◽

pp. 933 ◽

Cited By ~ 1

Author(s):

Joan Lloyd ◽

Johann Schröder ◽

David Rutley

Keyword(s):

Meat Products ◽

Economic Cost ◽

Meat Inspection ◽

Carcass Weight ◽

Economic Losses ◽

Human Consumption ◽

Bacterial Arthritis ◽

Climatic Zones ◽

Australian Standard ◽

Production Losses

Bacterial arthritis in sheep is a painful and debilitating condition that is widespread across all sheep-raising regions and climatic zones of Australia. At slaughter, bacterial arthritis in sheep causes economic losses through the condemnation of all or parts of the carcass, as specified in the Australian Standard for the Hygienic Production and Transportation of Meat and Meat Products for Human Consumption. In this study, we investigated the economic cost of arthritis in lambs presenting to an abattoir in southern Australia using a combination of the prevalence of arthritis detected during meat inspection, condemnation rates, trim weight and carcass weight, and fat measurements. Data were collected on 354 lines of lambs representing 63287 carcasses. One hundred and sixty nine consignments, or approximately one-half of the consignments, had at least one carcass with arthritis/polyarthritis detected by meat inspection personnel. Four hundred and twenty-two, or 0.7%, of the carcasses had arthritis/polyarthritis in at least one joint. When arthritis was present, on average 2.0% of the line was affected. Three carcasses with arthritis were condemned and the remainder trimmed, with an average trim weight of 0.7 kg. In addition, arthritis reduced the growth of lambs by 1.2 kg hot standard carcass weight, approximately 2.7 kg liveweight (assuming 45% dressing percentage) and reduced fat cover by 1.8 mm.

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Exposure of the Gulf of St. Lawrence grey seal Halichoerus grypus population to potentially zoonotic infectious agents

Diseases of Aquatic Organisms ◽

10.3354/dao03536 ◽

2020 ◽

Vol 142 ◽

pp. 105-118

Author(s):

CC Sauvé ◽

A Hernández-Ortiz ◽

E Jenkins ◽

F Mavrot ◽

A Schneider ◽

...

Keyword(s):

Meat Products ◽

Halichoerus Grypus ◽

Human Consumption ◽

Infectious Agents ◽

Erysipelothrix Rhusiopathiae ◽

Public Health Significance ◽

Domestic Livestock ◽

Health Significance ◽

Commercial Source ◽

Polymerase Chain

The population of grey seals Halichoerus grypus in Canadian waters is currently used as a commercial source of meat for human consumption. As with domestic livestock, it is important to understand the occurrence in these seals of infectious agents that may be of public health significance and thus ensure appropriate measures are in place to avoid zoonotic transmission. This study examined the prevalence of antibodies against Brucella spp., Erysipelothrix rhusiopathiae, 6 serovars of Leptospira interrogans, and Toxoplasma gondii in 59 grey seals and determined by polymerase chain reaction (PCR) the presence of these potentially zoonotic agents in specific organs and tissues of seropositive animals. The presence of encysted Trichinella spp. larvae was also investigated by digestion of tongue, diaphragm and other muscle samples, but none were detected. Seroprevalence against Brucella spp. and E. rhusiopathiae was low (5 and 3%, respectively). All 59 seals tested had antibodies against L. interrogans, but no carrier of this bacterium was detected by PCR. Seroprevalence against T. gondii was 53%, and DNA of this protozoan was detected by PCR in 11/30 (37%) seropositive animals. Standard sanitary measures mandatory for commercialization of meat products for human consumption should greatly reduce the potential for exposure to these infectious agents. However, special consideration should be given to freezing seal meat for at least 3 d to ensure destruction of tissue cysts of T. gondii.

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Edible Insects: Preliminary Study about Perceptions, Attitudes, and Knowledge on a Sample of Portuguese Citizens

Foods ◽

10.3390/foods10040709 ◽

2021 ◽

Vol 10 (4) ◽

pp. 709

Author(s):

Sofia G. Florença ◽

Paula M. R. Correia ◽

Cristina A. Costa ◽

Raquel P. F. Guiné

Keyword(s):

Nutritive Value ◽

Meat Products ◽

Regression Tree ◽

Good Alternative ◽

Classification And Regression Tree ◽

Categorical Variables ◽

Final Conclusion ◽

Edible Insects ◽

Chi Square ◽

Alternative Sources

This study investigated the knowledge, attitudes, consumption habits, and degree of acceptability of edible insects (EI) or derived products among Portuguese consumers. This work consisted of a questionnaire survey, undertaken on a sample of 213 participants. For the treatment of data, basic descriptive statistics were used, complemented with chi-square tests to assess some associations between categorical variables. Moreover, a tree classification analysis was carried out using a classification and regression tree (CRT) algorithm with cross-validation. The results indicated that people tend to have correct perceptions about the sustainability issues associated with the use of insects as alternative sources of protein; however, the level of knowledge and overall perception about their nutritive value is low. Regarding the consumption of EI, it was found that only a small part of the participants had already eaten them, doing it mostly abroad, by self-initiative, in a restaurant or at a party or event. Additionally, it was found that the reluctance to consume insects is higher if they are whole, but when they are transformed into ingredients used in food formulations, the level of acceptance increases. Furthermore, men have shown to have a better perception about EI, be more informed about sustainability, and have a higher level of acceptability when compared to women. As a final conclusion, it was observed that the Portuguese still show some resistance to adhere to the use of insects as replacements for meat products, but the market of insect based products can be a good alternative to overpass the neophobia associated with this type of food.

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Assessment of Poverty by Municipalities in the Context of Population Ageing—The Case of Małopolskie Voivodeship

Sustainability ◽

10.3390/su13052563 ◽

2021 ◽

Vol 13 (5) ◽

pp. 2563

Author(s):

Małgorzata Ćwiek ◽

Katarzyna Maj-Waśniowska ◽

Katarzyna Stabryła-Chudzio

Keyword(s):

The Elderly ◽

Research Problem ◽

Point Of View ◽

Computer Assisted ◽

Chi Square ◽

Social Challenge ◽

Depth Analysis ◽

Qualitative Variables ◽

Chi Square Test ◽

Research Gap

This article undertakes the research problem of the assessment of the significance of poverty as a social challenge for local self-government units, and the differences in the assessment of the incidence of this phenomenon depending on the type of municipality. The authors also analyse the relationships between the ageing of the population and the assessment of the extent of poverty by municipalities. It must be pointed out that the undertaken problem has not been a subject of in-depth analysis thus far. Hence, this article fills the identified research gap in this field. The empirical part is based on the results of our own research, conducted using the Computer-Assisted Web Interview (CAWI) method on a sample of 144 municipalities of the Małopolskie Voivodship (Poland). In order to verify whether there is a relationship between the researched qualitative variables, the chi-square test of independence was used. In order to determine the relationships occurring between the categories of variables characterising the scale of the incidence of poverty and the remaining variables, a correspondence analysis was conducted. The research enabled us to find the issue of poverty to be one of the most important social problems from the point of view of municipalities. It is also worth noting that the degree of ageing in the population has an impact on the assessment of poverty among the elderly.

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Occurrence and Survival of Verocytotoxin-Producing Escherichia coli O157 in Meats Obtained from Retail Outlets in The Netherlands

Journal of Food Protection ◽

10.4315/0362-028x-62.10.1115 ◽

1999 ◽

Vol 62 (10) ◽

pp. 1115-1122 ◽

Cited By ~ 51

Author(s):

A. E. HEUVELINK ◽

J. T. M. ZWARTKRUIS-NAHUIS ◽

R. R. BEUMER ◽

D E. de BOER

Keyword(s):

Escherichia Coli ◽

The Netherlands ◽

Meat Products ◽

Storage Period ◽

Processed Meat ◽

Raw Meat ◽

Pork Products ◽

Minced Beef ◽

Beef Products ◽

Meat Samples

In 1996 and 1997, 2,941 fresh and processed meat products obtained from supermarkets and butcher shops in The Netherlands were examined for the presence of verocytotoxin-producing Escherichia coli of serogroup O157 (O157 VTEC). Additionally, the fate of O157 VTEC in raw meat products stored at low temperatures and the effect of different additives were evaluated. O157 VTEC strains were isolated from 6 (1.1%) of 571 samples of raw minced beef, 2 (0.5%) of 402 samples of raw minced mixed beef and pork, 1 (1.3%) of 76 samples of raw minced pork, 1 (0.3%) of 393 samples of other raw pork products, and 1 (0.3%) of 328 samples of cooked or fermented ready-to-eat meats. Other raw beef products (n = 223) and meat samples originating from poultry (n = 819), sheep or lamb (n = 46), or wild animals (n = 83) were all found to be negative for O157 VTEC. For the survival experiments we used tartaar (minced beef with a fat content of less than 10%) and filet americain (tartaar mixed with a mayonnaise-based sauce [80 to 20%]). The O157 VTEC strain tested was able to survive in tartaar and filet americain stored at −20, 0, 5, or 7°C for 3 days. At both 7 and at 15°C, O157 VTEC counts in tartaar and filet americain remained virtually unchanged throughout a storage period of 5 days. Addition of acetic acid (to pH 4.0), sodium lactate (1 and 2% [wt/wt]), or components of the lactoperoxidase–thiocyanate–hydrogen peroxide system to filet americain did not result in a reduction of viable O157 VTEC cells during storage at 7 or 15°C. It was concluded that raw meat contaminated with O157 VTEC will remain a hazard even if the meat is held at low or freezing temperatures.

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Relationship between oral habits and spirometry maneuvers, in children

Revista CEFAC ◽

10.1590/1982-0216201820110517 ◽

2018 ◽

Vol 20 (1) ◽

pp. 37-43

Author(s):

Rafaela Coelho Minsky ◽

Tayná Castilho ◽

Roseane Rebelo Silva Meira ◽

Tatiana Godoy Bobbio ◽

Camila Isabel Santos Schivinski

Keyword(s):

Healthy Children ◽

Chi Square ◽

Cross Sectional ◽

Public And Private ◽

Oral Habits ◽

Qualitative Variables ◽

Chi Square Test ◽

Kolmogorov Smirnov ◽

Thumb Sucking ◽

Smirnov Test

ABSTRACT Purpose: to analyze whether deleterious oral habits can influence the number of attempts of forced spirometry maneuvers performed by healthy children. Methods: this observational and cross-sectional analytical study included 149 healthy children aged 6-12 years attending public and private schools in Florianópolis, SC, Brazil. A validated protocol was applied for the analysis of deleterious oral habits. The children were grouped according to the number of spirometry maneuvers needed to achieve successful spirometry results, as follows: G1) children who needed 3 maneuvers; G2) 4 maneuvers; G3) 5-8 maneuvers. Data were analyzed with the Kolmogorov-Smirnov test and the Kruskal-Wallis test was applied to compare quantitative variables between the groups. The Chi-square test was used to assess the association between the groups and qualitative variables. Results: there was no association between the number of attempts and the qualitative variables evaluated by the protocol. There was also no difference between the groups regarding quantitative variables for breastfeeding time, breastfeeding occurrence, use of pacifiers, and thumb sucking. Conclusion: the presence of DOH did not influence the number of forced spirometry maneuvers, performed by the healthy children in this study.

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Molecular and HPLC-based approaches for detection of aflatoxin B1 and ochratoxin A released from toxigenic Aspergillus species in processed meat

BMC Microbiology ◽

10.1186/s12866-021-02144-y ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Abdelazeem M. Algammal ◽

Mahmoud E. Elsayed ◽

Hany R. Hashem ◽

Hazem Ramadan ◽

Norhan S. Sheraba ◽

...

Keyword(s):

Ochratoxin A ◽

Meat Products ◽

Its Region ◽

Processed Meat ◽

Isolation And Identification ◽

Beef Meat ◽

Meat Samples ◽

Aflatoxin B ◽

Minced Meat ◽

Mold Count

Abstract Background Meat-products are considered an enriched media for mycotoxins. This study aimed to investigate the prevalence of toxigenic Aspergillus species in processed meat samples, HPLC-quantitative measurement of aflatoxin B1 and ochratoxin A residues, and molecular sequencing of aflR1 and pks genes. One hundred and twenty processed beef meat specimens (basterma, sausage, and minced meat; n = 40 for each) were collected from Ismailia Province, Egypt. Samples were prepared for total mold count, isolation, and identification of Aspergillus species. All samples were analyzed for the production of both Aflatoxin B1 and Ochratoxin A mycotoxins by HPLC. Molecular identification of Aspergillus flavus and Aspergillus ochraceus was performed using PCR amplification of the internal transcribed spacer (ITS) region; furthermore, the aflR1 and pks genes were sequenced. Results The total mold count obtained from sausage samples was the highest one, followed by minced meat samples. The prevalence of A. flavus was (15%), (7.5%), and (10%), while the prevalence of A. ochraceus was (2.5%), (10%), and (0%) in the examined basterma, sausage, and minced meat samples, respectively. Using PCR, the ITS region was successfully amplified in all the tested A. flavus and A. ochraceus strains. Aflatoxin B1 was detected in six basterma samples (15%). Moreover, the ochratoxin A was detected only in four sausage samples (10%). The aflR1 and pks genes were amplified and sequenced successfully and deposited in the GenBank with accession numbers MF694264 and MF694264, respectively. Conclusions To the best of our knowledge, this is the first report concerning the HPLC-Molecular-based approaches for the detection of aflatoxin B1 and ochratoxin A in processed beef meat in Egypt. The production of aflatoxin B1 and ochratoxin A in processed meat constitutes a public health threat. Aflatoxin B1 is commonly associated with basterma samples. Moreover, ochratoxin A was detected frequently in sausage samples. The routine inspection of mycotoxins in processed meat products is essential to protect human consumers.

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