scholarly journals Evaluation of oxidative and an­tioxidant status in dairy calves before and after weaning

2021 ◽  
Vol 24 (2) ◽  
pp. 184-190
Author(s):  
A. Majlesi ◽  
S. P. Yasini ◽  
S. Azimpour ◽  
P. Mottaghian
Keyword(s):  
Oxidative Stress ◽  
Blood Cells ◽  
Dairy Calves ◽  
Weaning Stress ◽  
Vitamins E And C ◽  
Healthy Female ◽  
Before And After ◽  
Holstein Calves ◽  
Oxidative System ◽  
Calf Rearing

Weaning is a stressful step in calf rearing which can lead to oxidative stress. The purpose of the present study was to investigate oxidative stress and antioxidant status in calves pre and post weaning. A total of 22 clinically healthy female Holstein calves at the same age were selected and their blood samples were examined to measure the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) in red blood cells and serum malondialdehyde (MDA) concentration. Sampling was performed in 4 consecutive stages: 7 days pre-weaning, the day of weaning, 24 and 72 hours after it. The results indicated that the activity of SOD and GPx enzymes increased significantly on the weaning day compared to the previous week. CAT activity increased 24 and 72 hours after weaning and its increase was significant 72 hours after weaning compared to the day of weaning. An increase was observed in MDA level on the day of weaning compared to a week before, and the trend was towards increase after the weaning. Weaning stress leads to disturbances of oxidative system balance and causes oxidative damage in calves. This imbalance can be probably resolved by improving the antioxidant system and supplementing antioxidants such as vitamins E and C against free radicals produced during weaning.

scholarly journals Welfare assessment of calf rearing management practices in family-based dairy units in rural areas of Mymensingh district, Bangladesh

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
M. A. Islam ◽  
S. A. Shanta ◽  
R. A. Lima ◽  
M. Milon ◽  
K. Rudra
Keyword(s):  
Scientific Knowledge ◽  
Rural Areas ◽  
Management Practices ◽  
Vaccination Schedule ◽  
Dairy Calves ◽  
Traditional Practices ◽  
Before And After ◽  
Family Based ◽  
Poor Welfare ◽  
Calf Rearing

Background: Dairy calves are the future stock of the dairy farms. It is important to describe weaknesses in rearing calves not only to improve their welfare, but also to detect areas where current scientific knowledge is poorly integrated into practice. The aim of the present study was to gather information on calf rearing management practices followed by dairy owners, which are related to welfare. Methods: A survey of calf rearing practices was conducted using a farmer questionnaire to collect data. The survey included 140 family-based small dairy units in seven upazilas of Mymensingh district, Bangladesh. The frequency and percentage of the response were described. Results: We observed that all respondents attended their animals during calving and 82.14% respondents allowed cow to clean the calves immediately after calving. Only 13.57% farmers cleaned the mouth and nose of calf after birth. More than 85.0% owners cleaned and trimmed hooves of the calf after birth. More than 90%% respondents did not cut the naval cord of calf and apply antiseptic. More than half of the owners (54.28%) fed colostrum to calves when the animals were able to stand on their feet and only 37.14% of them fed colostrum within one hour of birth. About 44.28% respondents allowed ad lib quantity of colostrum to calf. It was found that 73.57% of the owners allowed suckling of calf until 3 to 6 month of age. Most of the owners (55.0%) allowed the calves to suckle all the quarter of udder before and after milking. None practiced castration and dehorning for male calves. Majority (97.14%) of farmers did not perform deworming and vaccination schedule of calves. Conclusion: This study identified a number of rearing practices that represent a poor welfare for calf, which may prone towards diseases and hampering the productivity. Therefore, in addition to the traditional practices, scientific knowledge for this aspect is also important to get superior animals for future.

scholarly journals P0946OXIDATIVE STRESS AND HEME METABOLISM IN RED BLOOD CELLS FROM HEMODIALYSIS PATIENTS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Gabriela Ferreira Dias ◽  
Gabriela Bohnen ◽  
Nadja Grobe ◽  
Xia Tao ◽  
Roberto Pecoits-Filho ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Organic Anion ◽  
Fold Increase ◽  
Organic Anion Transporter ◽  
Ros Generation ◽  
Heme Oxygenase 1 ◽  
Anion Transporter ◽  
Before And After

Abstract Background and Aims We have previously described that indoxyl sulfate promotes red blood cells (RBC) ROS generation through organic anion transporter 2 as well as NADPH oxidase activity-dependent and GSH-independent mechanisms (Dias et al., 2018). However, there is little information regarding pathways of antioxidant balance to protect RBC from extensive oxidative stress that occurs during hemodialysis (HD). Intracellular free heme is degraded by Heme Oxygenase 1 (HO-1), which is regarded as the major cytoprotective enzyme (Maines, 1988; Gozzelino et al., 2010). In the current study, we assessed HO-1 activity and ROS production in RBC from healthy subjects and hemodialysis (HD) patients before and after HD. Method Blood was drawn from 6 healthy individuals (CON-RBC) and 6 HD patients (HD-RBC) before (pre/HD-RBC) and after high flux HD (post/HD-RBC). Isolated RBC were stained with DCFH-DA (Abcam) for ROS measurements. To quantify HO-1, RBC were incubated with anti-HO-1 antibody (Abcam) and m-IgGκ BP-CFL 488 (Santa Cruz Biotechnology) as a secondary antibody. Samples were analyzed by flow cytometry. Results Our results show a 4-fold increase in ROS levels in pre/HD-RBC compared to CON-RBC. ROS levels were even further increased by 1.65-fold after HD treatment in post/HD-RBC (Figure 1). Both pre/HD-RBC and post/HD-RBC showed a similarly significant increase of 3.3-fold in HO-1 compared to CON-RBC. (Figure 1). Conclusion High levels of HO-1 may represent a defense against oxidative stress that occurs in ESKD and particularly during the HD session. Further research is needed to evaluate whether HO-1 overexpression could accelerate heme degradation and contribute to renal anemia.

Oxidative stress and rheologic properties of stored red blood cells before and after transfusion to surgical patients

Transfusion ◽  
2016 ◽  
Vol 56 (5) ◽  
pp. 1101-1111 ◽  
Author(s):  
Enika Nagababu ◽  
Andrew V. Scott ◽  
Daniel J. Johnson ◽  
Ian M. Dwyer ◽  
Joshua A. Lipsitz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Surgical Patients ◽  
Before And After ◽  
Rheologic Properties

scholarly journals Pessimistic dairy calves are more vulnerable to pain-induced anhedonia

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242100
Author(s):  
Benjamin Lecorps ◽  
Emeline Nogues ◽  
Marina A. G. von Keyserlingk ◽  
Daniel M. Weary
Keyword(s):  
Individual Differences ◽  
Local Anesthesia ◽  
Individual Variation ◽  
Dairy Calves ◽  
Judgment Bias ◽  
Sweet Solution ◽  
Before And After ◽  
Holstein Calves ◽  
Rule Out

Pain induces deficits in appreciation of rewards (i.e. anhedonia) and variation in response to pain may be partly explained by individual differences in general expectations (i.e. optimism). Dairy calves are routinely subjected to painful procedures such as hot-iron disbudding. We tested if female Holstein calves (n = 17) display signs of anhedonia (as evidenced by reduced consumption of a sweet solution) after hot-iron disbudding (performed under general and local anesthesia), and whether individual differences in optimism explain the variation in this response. Individual variation in optimism was measured using responses to two judgment bias tests (performed when calves were 25 d old), and anhedonia was measured by comparing consumption of a sweet solution before and after hot-iron disbudding. We found that intake of the sweet solution declined (by mean ± SD: 48.4 ± 44.3%) on the day after disbudding, and that more pessimistic calves were more affected. Sweet solution consumption did not return to baseline for the duration of the study (i.e. 5 days). Calves reduced their intake of a sweet solution after hot-iron disbudding, consistent with pain-induced anhedonia, and more pessimistic calves showed stronger evidence of anhedonia, suggesting that they were more affected by the procedure. However, our results cannot rule out the possibility that calf responses were driven by anorexia.

Oxidative Stress Status in Patients with Choledocholithiasis: Before and After Endoscopic Sphincterotomy and Biliary Clearance

2018 ◽  
Vol 69 (8) ◽  
pp. 2172-2176
Author(s):  
Catalin Victor Sfarti ◽  
Alin Ciobica ◽  
Carol Stanciu ◽  
Gheorghe G. Balan ◽  
Irina Garleanu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endoscopic Sphincterotomy ◽  
Specific Activity ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Oxidative Stress Parameter ◽  
Extrahepatic Cholestasis ◽  
Biliary Clearance ◽  
Oxidative Stress Status ◽  
Before And After

Choledocholithiasis may cause biliary obstruction which leads to hepatocellular injury. Oxidative stress has been proposed as a possible mechanism involved in this disorder. This study evaluates the oxidative stress burden in patients with choledocholithiasis and secondary cholestasis, before and after endoscopic sphincterotomy. Experimental part: Patients diagnosed with choledocholithiasis and secondary extrahepatic cholestasis were included in the study between January 1st 2016 and October 31st 2016. In all patients oxidative stress markers were collected within 2 hours before and 48 hours after therapeutic ERCP. Selected markers were superoxide dismutase (SOD), glutathione peroxidase (GPX) and malondialdehyde (MDA). The results were compared to those from a group of 40 healthy subjects. Significantly lower concentrations of SOD (p = 0.03) and GPX (p [ 0.0001) activities, associated with an increased level of MDA level (p [ 0.0001) were shown in patients before biliary clearance compared with the healthy control group. After ERCP the only oxidative stress parameter which showed improvement was the SOD specific activity (p = 0.037). This study shows that extrahepatic cholestasis secondary to choledocholithiasis is associated with increased oxidative stress status. After biliary clearance one oxidative stress marker was significantly improved (SOD), suggesting a possible antioxidant effect of such procedure.

Evidences of Brain Plasticity and Motor Control Modulation after Hemodialysis Session by Helixone Membrane: BOLD-fMRI Study

2020 ◽  
Vol 19 (6) ◽  
pp. 466-477
Author(s):  
Saïd Boujraf ◽  
Rachida Belaïch ◽  
Abdelkhalek Housni ◽  
Badreeddine Alami ◽  
Tariq Skalli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Functional Activity ◽  
Brain Plasticity ◽  
Biological Assessment ◽  
Bold Fmri ◽  
Before And After ◽  
Total Antioxidant ◽  
Inflammatory State ◽  
Functional Control ◽  
The Impact

Objective: The aim of this paper is to demonstrate the impact of hemodialysis (HD) using synthetic Helixone membrane on brain functional control reorganization and plasticity in the cortical area generated while Oxidative Stress (OS) would be the main impacting agent. Methods: Indeed, 9 chronic HD patients underwent identical brain BOLD-fMRI assessment using the motor paradigm immediately before and after the same HD sessions. To assess the oxidative stress, the same patients underwent biological-assessment, including Malondialdehyde (MDA) and Total- Antioxidant-Activity (TAOA) reported in earlier papers. Results: BOLD-fMRI maps of motor areas obtained from HD-patients before and after HD sessions revealed a significant enhancement of activation volume of the studied motor cortex after HD reflecting brain plasticity. Results were correlated with OS assessed by the measurement of MDA and TAOA; this correlation was close to 1. Conclusion: Indeed, HD enhances the inflammatory state of brain tissues reflected by the increased OS. The functional brain reaction demonstrated a functional activity reorganization to overcome the inflammatory state and OS enhanced by HD process. This functional activity reorganization reveals brain plasticity induced by OS originated by HD.

scholarly journals Lysine and Methionine Supplementation for Dairy Calves Is More Accurate through the Liquid than the Solid Diet

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 332
Author(s):  
Jackeline Thais Silva ◽  
Evangelina Miqueo ◽  
Thaís Manzoni Torrezan ◽  
Nathalia Brito Rocha ◽  
Giovana Simão Slanzon ◽  
...  
Keyword(s):  
Feed Intake ◽  
Dairy Calves ◽  
Body Measurements ◽  
Negative Effects ◽  
Intermediate Metabolism ◽  
Lower Body Weight ◽  
Solid Diet ◽  
Holstein Calves ◽  
Methionine Supplementation ◽  
Daily Intakes

This study aimed to evaluate the performance and metabolic changes in dairy calves supplemented with lysine and methionine in milk replacer (MR) or starter concentrate (SC). Male Holstein calves (n = 45) were blocked and distributed in Control without supplementation (1) and; Lysine and Methionine supplementation to achieve an intake of 17 and 5.3 g/d in the SC (2) and to achieve of 17 and 5.3 g/d in the MR (3). MR was fed (6 L/d) until the 8th week of life when weaning occurred. Calves were followed until the 10th week of age. Feed intake was measured daily. Weight and body measurements were registered weekly. Blood samples were collected biweekly to evaluate the intermediate metabolism. The AA supplementation resulted in lower body weight at weaning and week 10. Calves fed SC Lys:Met had lower SC intake and lower total feed intake at weaning when compared to control. Calves fed control had higher heart girth, hip-width, and plasma glucose concentration. The supplementation with Lys and Met did not benefit dairy calves’ performance nor metabolism in this study. Supplementation through the MR was more efficient than SC to result in adequate daily intakes of AA. Further studies are needed to understand the negative effects of AA on calf starter intake.

scholarly journals PSIX-6 Gastrointestinal microbiome of calves fed solid diets containing different levels and sources of NDF

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 418-419
Author(s):  
Gercino F Virgínio Júnior ◽  
Milaine Poczynek ◽  
Ana Paula Silva ◽  
Ariany Toledo ◽  
Amanda Cezar ◽  
...  
Keyword(s):  
Illumina Miseq ◽  
Diversity Indices ◽  
Dairy Calves ◽  
Rrna Gene ◽  
Gastrointestinal Microbiome ◽  
Fecal Microbiome ◽  
Miseq Platform ◽  
Holstein Calves ◽  
Ad Libitum ◽  
Different Levels

Abstract Different levels and sources of NDF can modify the gastrointestinal microbiome. This study evaluated 18 Holstein calves housed in not-bedded suspended individual cages and fed one of three treatments: 22NDF - conventional starter containing 22% NDF (n = 7); 31NDF - starter with 31% NDF, replacing part of the corn by soybean hull (n = 6); and 22NDF+H - conventional starter with 22% NDF plus coast-cross hay ad libitum (n = 5). All animals received 4 L of milk replacer daily (24% CP; 18.5% fat; diluted to 12.5% solids), divided into two meals, being weaned at 8th week of age. After weaning, animals were housed in tropical shelters, fed with the respective solid diet and coast-cross hay ad libitum for all treatments. To evaluate the microbiome, ruminal fluid samples were collected using a modified Geishauser oral probe at weeks 2, 4, 6, 8 and 10, two hours after the morning feeding, and fecal samples were collected at birth (0) and at weeks 1, 2, 4, 8 and 10. The microbial community was determined by sequencing V3 and V4 region amplicons of the 16S rRNA gene that was amplified by PCR and sequenced by the Illumina MiSeq platform. Ruminal microbiome had no differences in diversity for the effects of weeks, treatments or interaction of both factors (Table 1). In feces, the diversity indices and evenness were higher for 22NDF+H when compared to 22NDF, with no difference for 31NDF. All indices were significantly affected by calves age. At birth, calves had the greatest diversity and richness. Week 1 and 2 had less evenness and diversity. Bacteroidota, Firmicutes_A and Firmicutes_C were the most abundant phylum in rumen and feces. The supply of hay was only effective in modifying the fecal microbiome of dairy calves, suggesting a resilience in the ruminal microbiome.

scholarly journals Oxidative Stress Compromises Lymphocyte Function in Neonatal Dairy Calves

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 255
Author(s):  
Wilmer Cuervo ◽  
Lorraine M. Sordillo ◽  
Angel Abuelo
Keyword(s):  
Oxidative Stress ◽  
Immune Responses ◽  
Immune Cell ◽  
Lymphocyte Activation ◽  
Dairy Calves ◽  
Lymphocyte Function ◽  
Newborn Calves ◽  
Ifn Γ ◽  
The Impact

Dairy calves are unable to mount an effective immune response during their first weeks of life, which contributes to increased disease susceptibility during this period. Oxidative stress (OS) diminishes the immune cell capabilities of humans and adult cows, and dairy calves also experience OS during their first month of life. However, the impact that OS may have on neonatal calf immunity remains unexplored. Thus, we aimed to evaluate the impact of OS on newborn calf lymphocyte functions. For this, we conducted two experiments. First, we assessed the association of OS status throughout the first month of age and the circulating concentrations of the cytokines interferon-gamma (IFN-γ) and interleukin (IL) 4, as well as the expression of cytokine-encoding genes IFNG, IL2, IL4, and IL10 in peripheral mononuclear blood cells (PBMCs) of 12 calves. Subsequently, we isolated PBMCs from another 6 neonatal calves to investigate in vitro the effect of OS on immune responses in terms of activation of lymphocytes, cytokine expression, and antibody production following stimulation with phorbol 12-myristate 13-acetate or bovine herpesvirus-1. The results were compared statistically through mixed models. Calves exposed to high OS status in their first month of age showed higher concentrations of IL-4 and expression of IL4 and IL10 and lower concentrations of IFN-γ and expression of IFNG and IL2 than calves exposed to lower OS. In vitro, OS reduced lymphocyte activation, production of antibodies, and protein and gene expression of key cytokines. Collectively, our results demonstrate that OS can compromise some immune responses of newborn calves. Hence, further studies are needed to explore the mechanisms of how OS affects the different lymphocyte subsets and the potential of ameliorating OS in newborn calves as a strategy to augment the functional capacity of calf immune cells, as well as enhance calves’ resistance to infections.

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