Towards a Positive Welfare Protocol for Cattle: A Critical Review of Indicators and Suggestion of How We Might Proceed

Current animal welfare protocols focus on demonstrating the absence (or at least low levels) of indicators of poor welfare, potentially creating a mismatch between what is expected by society (an assurance of good animal welfare) and what is actually being delivered (an assurance of the absence of welfare problems). This paper explores how far we have come, and what work still needs to be done, if we are to develop a protocol for use on commercial dairy farms where the aim is to demonstrate the presence of positive welfare. Following conceptual considerations around a perceived “ideal” protocol, we propose that a future protocol should be constructed (i) of animal-based measures, (ii) of indicators of affective state, and (iii) be structured according to indicators of short-term emotion, medium-term moods and long-term cumulative assessment of negative and positive experiences of an animal's life until now (in contrast to the current focus on indicators that represent different domains/criteria of welfare). These three conditions imposed the overall structure within which we selected our indicators. The paper includes a critical review of the literature on potential indicators of positive affective states in cattle. Based on evidence about the validity and reliability of the different indicators, we select ear position, play, allogrooming, brush use and QBA as candidate indicators that we suggest could form a prototype positive welfare protocol. We emphasise that this prototype protocol has not been tested in practice and so it is perhaps not the protocol itself that is the main outcome of this paper, but the process of trying to develop it. In a final section of this paper, we reflect on some of the lessons learnt from this exercise and speculate on future perspectives. For example, while we consider we have moved towards a prototype positive welfare protocol for short-term affective states, future research energy should be directed towards valid indicators for the medium and long-term.

Pet Reptiles—Are We Meeting Their Needs?

The ability to meet the needs of each species in captivity is at the heart of the ethical debate on the acceptability of keeping reptiles and other animals as pets. Little is known about the ability of reptile owners to understand their pets’ behavior and to meet their welfare requirements. In this study, we surveyed pet reptile owners in Portugal (N = 220) to assess their behavioral knowledge and the provision of essential husbandry needs. Although two-thirds of respondents (68%) scored very good to excellent in terms of knowledge of their pet reptile’s behaviors, only 15% of respondents met four essential reptile husbandry needs (temperature, lighting, diet and refuge) and 43% met two or less. None of the respondents reported their reptile’s welfare as very poor, and only a single respondent reported it as poor. Logistic regression model showed that while snake owners had fourteen times higher odds of reporting adequate husbandry provision, lizard owners had the highest odds of reporting good or very good welfare despite providing less of their animals’ basic husbandry needs. These results suggest that many pet reptiles in Portugal live in, at best, ‘controlled deprivation’ and are at risk of suffering poor welfare throughout their captive lives. Moreover, behaviors indicative of poor welfare and captivity stress were considered ‘normal’ by up to one quarter of respondents. We suggest that the frequency of these behaviors in pet reptiles has led to their acceptance as normal, precluding the search for ways to prevent them. These results suggest that campaigns aimed at challenging the current norm for adequate reptile welfare are warranted.

Can animals develop depression? An overview and assessment of ‘depression-like’ states

Describing certain animal behaviours as ‘depression-like’ or ‘depressive’ has become common across several fields of research. These typically involve unusually low activity or unresponsiveness and/or reduced interest in pleasure (anhedonia). While the term ‘depression-like’ carefully avoids directly claiming that animals are depressed, this narrative review asks whether stronger conclusions can be legitimate, with animals developing the clinical disorder as seen in humans (cf., DSM-V/ICD-10). Here, we examine evidence from animal models of depression (especially chronically stressed rats) and animals experiencing poor welfare in conventional captive conditions (e.g., laboratory mice and production pigs in barren environments). We find troubling evidence that animals are indeed capable of experiencing clinical depression, but demonstrate that a true diagnosis has yet to be confirmed in any case. We thus highlight the importance of investigating the co-occurrence of depressive criteria and discuss the potential welfare and ethical implications of animal depression.

Nature calls: intelligence and natural foraging style predict poor welfare in captive parrots

Understanding why some species thrive in captivity, while others struggle to adjust, can suggest new ways to improve animal care. Approximately half of all Psittaciformes, a highly threatened order, live in zoos, breeding centres and private homes. Here, some species are prone to behavioural and reproductive problems that raise conservation and ethical concerns. To identify risk factors, we analysed data on hatching rates in breeding centres (115 species, 10 255 pairs) and stereotypic behaviour (SB) in private homes (50 species, 1378 individuals), using phylogenetic comparative methods (PCMs). Small captive population sizes predicted low hatch rates, potentially due to genetic bottlenecks, inbreeding and low availability of compatible mates. Species naturally reliant on diets requiring substantial handling were most prone to feather-damaging behaviours (e.g. self-plucking), indicating inadequacies in the composition or presentation of feed (often highly processed). Parrot species with relatively large brains were most prone to oral and whole-body SB: the first empirical evidence that intelligence can confer poor captive welfare. Together, results suggest that more naturalistic diets would improve welfare, and that intelligent psittacines need increased cognitive stimulation. These findings should help improve captive parrot care and inspire further PCM research to understand species differences in responses to captivity.

The in-utero experience of piglets born from sows with lameness shapes their life trajectory

Experiences during gestation can alter the mother’s behavior and physiology, thereby potentially affecting the behavioral and physiological development of the offspring. In livestock, one common challenge for pregnant animals is lameness: a multifactorial condition that causes pain, stress, resulting in poor welfare outcomes. Since maternal pain can affect offspring development, we aimed to quantify the behavioral response in 142 piglets born from sows with different degrees of lameness during pregnancy. Gait scores of 22 pregnant group-housed sows were assessed six times at 2-week intervals. Lameness scores varied from 0 (no lameness) to 5 (most severe lameness score). Saliva samples and behavior were assessed in the sows throughout pregnancy. Sows were moved to individual farrowing pens and placental tissue was collected for glucocorticoid assessment. At 28 days of age, piglets were weaned, weighed, and regrouped by body size and sex. Skin lesions were counted for each piglet on days 28, 29, and 30 after birth. During open field and novel object tests on day 30, the vocalization and activity levels were evaluated. Piglet data were grouped by the lameness score of the sows as G1 (without lameness), G2 (moderate lameness), and G3 (severe lameness). Data analysis included ANOVA or Kruskal–Wallis tests and pairwise comparisons which were performed using Tukey and Kramer (Nemenyi) test with Tukey-Dist approximation for independent samples. G2 piglets were heavier than G3 at weaning. G1 piglets had fewer skin lesions at days 28 and 29 than G2 piglets. Moreover, G1 piglets vocalized more than G2 when they were subjected to the combined open field and novel object test. We did not identify differences among sows showing different lameness scores in the concentration of placental or salivary glucocorticoids. Lameness in pregnant sows altered the offspring’s weight gain, number of skin lesions and vocalizations, together showing evidence that lameness in sows affect offspring performance and behavior.

Longitudinal national-level monitoring of on-farm broiler welfare identifies consistently poorly performing farms

A range of welfare outcome measures relating to on-farm welfare are monitored in UK slaughterhouses to check compliance with the European Broiler Directive. A national dataset from 438,155 batches of chickens between 2010 and 2014 and from 228,795 batches between 2016 and 2018 was analysed. The data contained information about 3.1 billion chickens. The highest mean proportion for a single condition was for ascites/oedema in 2016–2018 at 0.384%, affecting 3.9 million chickens/year sent to slaughter during that time, followed by abnormal colour/fevered at 0.324%, affecting 3.4 million chickens/year. Identifying farms most likely to have poor welfare is an important strategy for improving animal welfare overall, and for maximising the capacity for checking regulatory compliance when resources are limited. We found a greater proportion of broiler farms overall remained consistently in the best quartile (16.4%) rather than the worst quartile (6.6%). Farms that exceeded a Government ‘trigger’ threshold for poor welfare were significantly more likely to subsequently improve than ‘non-trigger’ farms, although they usually remained in the worst performing quartile of farms.

Animal sales from Wuhan wet markets immediately prior to the COVID-19 pandemic

Here we document 47,381 individuals from 38 species, including 31 protected species sold between May 2017 and November 2019 in Wuhan’s markets. We note that no pangolins (or bats) were traded, supporting reformed opinion that pangolins were not likely the spillover host at the source of the current coronavirus (COVID-19) pandemic. While we caution against the misattribution of COVID-19’s origins, the wild animals on sale in Wuhan suffered poor welfare and hygiene conditions and we detail a range of other zoonotic infections they can potentially vector. Nevertheless, in a precautionary response to COVID-19, China’s Ministries temporarily banned all wildlife trade on 26th Jan 2020 until the COVID-19 pandemic concludes, and permanently banned eating and trading terrestrial wild (non-livestock) animals for food on 24th Feb 2020. These interventions, intended to protect human health, redress previous trading and enforcement inconsistencies, and will have collateral benefits for global biodiversity conservation and animal welfare.

Sustainable agriculture: the use of FAMACHA method in Santa Ines sheep in the Semi-arid region of Brazil

Sheep farming is of great importance in generating income to smallholders in semi-arid areas worldwide. Gastrointestinal helminths cause important damage to the animals with poor welfare conditions, impacting in local economies. The objective of this work was to evaluate the FAMACHA method (FMC) in a herd of 60 Santa Inês from Paraíba, Brazil, and to compare the data from previous production years. In order to determine anemic sheep, FMC chart was used in biweekly evaluations for 12 months by trained personnel. Blood and fecal samples were collected monthly to determine packed cell volume (PCV), and parasite fecal egg count (FEC), respectively. The animals were divided into: dry, pregnant and lactating ewes, lambs and rams. Haemonchus was the predominant helminth genus (94.7%) during the whole year. There were up to 97% of success on the interpretation of FMC with 90 to 100% efficacy in the treatment decision. Among the 1356 evaluations, in only 40 (3%) were required deworming, being 27 during the rainy season and 13 during the long dry season. A statistically significant correlation (P < 0.01) was observed between FEC and PCV for the dry, lactating, and pregnant categories. The data reports a 94% reduction in deworming, with a greater need for anthelmintic use in lambs and lactating ewes (P < 0.05). Our data validates the use of FMC in Santa Inês sheep, which is considered a local resilient breed in the semi-arid region of Brazil. We recommend that animals should be examined during the rainy season to prevent most of the clinical problems.

Factors Affecting the Welfare of Unweaned Dairy Calves Destined for Early Slaughter and Abattoir Animal-Based Indicators Reflecting Their Welfare On-Farm

In many dairy industries, but particularly those that are pasture-based and have seasonal calving, “surplus calves,” which are mostly male, are killed at a young age because they are of low value and it is not economically viable to raise them. Such calves are either killed on farm soon after birth or sent for slaughter at an abattoir. In countries where calves are sent for slaughter the age ranges from 3-4 days (New Zealand and Australia; “bobby calves”) to 3-4 weeks (e.g., Ireland); they are not weaned. All calves are at the greatest risk of death in the 1st month of life but when combined with their low value, this makes surplus calves destined for early slaughter (i.e., &lt;1 month of age) particularly vulnerable to poor welfare while on-farm. The welfare of these calves may also be compromised during transport and transit through markets and at the abattoir. There is growing recognition that feedback to farmers of results from animal-based indicators (ABI) of welfare (including health) collected prior to and after slaughter can protect animal welfare. Hence, the risk factors for poor on-farm, in-transit and at-abattoir calf welfare combined with an ante and post mortem (AM/PM) welfare assessment scheme specific to calves &lt;1 month of age are outlined. This scheme would also provide an evidence base with which to identify farms on which such animals are more at risk of poor welfare. The following ABIs, at individual or batch level, are proposed: AM indicators include assessment of age (umbilical maturity), nutritional status (body condition, dehydration), behavioral status (general demeanor, posture, able to and stability while standing and moving, shivering, vocalizations, oral behaviors/cross-sucking, fearfulness, playing), and evidence of disease processes (locomotory ability [lameness], cleanliness/fecal soiling [scour], injuries hairless patches, swellings, wounds], dyspnoea/coughing, nasal/ocular discharge, navel swelling/discharge); PM measures include assessment of feeding adequacy (abomasal contents, milk in rumen, visceral fat reserves) and evidence of disease processes (omphalitis, GIT disorders, peritonitis, abscesses [internal and external], arthritis, septicaemia, and pneumonia). Based on similar models in other species, this information can be used in a positive feedback loop not only to protect and improve calf welfare but also to inform on-farm calf welfare management plans, support industry claims regarding animal welfare and benchmark welfare performance nationally and internationally.

A New, Practical Animal Welfare Assessment for Dairy Farmers

The Welfare Quality® assessment protocol (WQ) is the most extensive way to measure animal welfare. This study was set up to determine if resource-based welfare indicators, that are easier and faster to measure, could replace the more time consuming, animal-based measurements of the WQ. The WQ was applied on 60 dairy farms in the Netherlands, with good, moderate and poor welfare. The WQ protocol classified most farms (87%) as ‘acceptable’. Several of the animal-based measures of WQ correlated well with measures in the environment. Using these correlations, an alternative welfare assessment protocol (new Welfare Monitor) was designed, which takes approximately 1.5 h for a farm with 100 dairy cows. Because the opinion of farmers about welfare assessment is important if one wants to improve conditions for the cows at a farm, another objective of this study was to evaluate the usefulness of the new Welfare Monitor for the farmer. Over two years, the farms were visited, and advice was given to improve the conditions at the farm. After the first welfare assessment and advice, farmers improved the conditions for their cows substantially. Farms where the category score had increased made more improvements on average than those that did not upgrade.