scholarly journals The impact of different commercial probiotic cultures with starters on technological, physicochemical and sensorial properties of a traditional yogurt-based appetizer “Cacık”

2019 ◽  
Vol 69 (3) ◽  
pp. 193-205
Author(s):  
Seda Altuntas ◽  
Mihriban Korukluoglu
Keyword(s):  
Bifidobacterium Longum ◽  
Starter Culture ◽  
Lactobacillus Rhamnosus ◽  
Storage Period ◽  
Viable Cell ◽  
Probiotic Bacteria ◽  
Fermentation Time ◽  
Cell Counts ◽  
Sensorial Attributes ◽  
The Impact

The selection of proper probiotic culture is essential for maintaining adequate numbers of viable cells until consumption since potential adverse interaction between selected strain and starter culture. This study examined the suitability of Cacık as a potential probiotic carrier including Lactobacillus acidophilus 74-2, Lactobacillus rhamnosus Howaru HN001TM and Bifidobacterium longum BB536 in different combinations. The presence of three probiotic strains caused higher post acidification and shorter fermentation time associated to higher counts of L. bulgaricus. Except for certain combinations containing B. longum BB536, the counts of individual probiotic bacteria were higher than 6 log g-1 for all probiotic supplemented Cacık samples. Results showed that bacterial interaction was decisive for survival over 21 days of storage. Probiotic products containing only B. longum BB536 or B. longum BB536 with L. acidophilus 74-2 did not accomplish in terms of the technological point, since the viable cell counts decerased for 2.45 log and 1.95 log per g, respecitevly, throughout the storage period. In three of totally four combinations included L. rhamnosus Howaru HN001TM alone or combined with other probiotic bacteria, the viable cell counts of L. rhamnosus Howaru HN001TM remained at the inoculated level, while the counts significantly increased in co-culture with L. acidophilus 74-2. Considering the overall sensorial attributes and survival of probiotics, Cacık supplemented with L. rhamnosus Howaru HN001TM alone, combinations of B. longum BB536 and L. rhamnosus Howaru HN001TM or L. acidophilus 74-2 and L. rhamnosus Howaru HN001TM or by all of the three tested probiotics, were suggested as suitable for further production.

scholarly journals Fermented Cranberry Fortified Buckwheat Product—Phenolic Composition, Antioxidant and Microbiological Properties

2021 ◽  
Vol 11 (19) ◽  
pp. 9241
Author(s):  
Anna Mikulajová ◽  
Zuzana Matejčeková ◽  
Silvia Mošovská ◽  
Zlatica Kohajdová ◽  
Ľubomír Valík ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Positive Impact ◽  
Starter Culture ◽  
Lactobacillus Rhamnosus ◽  
Storage Period ◽  
Probiotic Strain ◽  
Experimental Period ◽  
Viable Cell ◽  
Total Phenolic ◽  
Cell Counts

This study determined the effect of fermentation by Fresco DVS 1010 starter culture with added probiotic strain Lactobacillus rhamnosus GG and potentially probiotic isolate Lactobacillus plantarum HM1, at fermentation times (0, 8 h) and cold storage period (24 h, 4th day, 7th day, 14th day), on microbial parameters, pH changes, total phenolic content, phenolic compounds profile, and antioxidant activity of buckwheat water- and milk-based mashes, flavored with cranberries and unflavored. The tested starter Fresco culture effectively fermented the buckwheat products and the viable cell counts of potentially probiotic bacteria were sufficient to demonstrate the health-promoting properties of final products. Lactic acid bacteria had a positive impact on total phenolic compound content, total flavonoid content, and antioxidant activity of buckwheat mashes, whereby final values (14 days) were higher by about 16.9–130.8%, 13.4–37.7%, and 14.5–145.9%, respectively, in comparison to initial values (0 h). Seven phenolic acids (gallic, protocatechuic, vanillic, syringic, caffeic, p-coumaric, and ferulic) and two flavonoids (rutin and quercetin) in buckwheat mashes were measured during the experimental period. The content of quercetin, gallic, and protocatechuic acids increased and, conversely, p-coumaric acid decreased, in all products. Prepared buckwheat fermented products have the potential to meet the criteria for potentially functional foods.

scholarly journals Fermentation Profile and Probiotic-Related Characteristics of Bifidobacterium longum MC-42

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 101
Author(s):  
Anna V. Begunova ◽  
Irina V. Rozhkova ◽  
Olga A. Glazunova ◽  
Konstantin V. Moiseenko ◽  
Olga S. Savinova ◽  
...  
Keyword(s):  
Protein Hydrolysate ◽  
Bifidobacterium Longum ◽  
Starter Culture ◽  
Antibiotic Resistance Genes ◽  
Viable Cell ◽  
Cultural Medium ◽  
Fermentation Profile ◽  
Growth Promoting ◽  
The Impact ◽  
Antihypertensive Properties

This article presents new data on Bifidobacterium longum MC-42—a strain that has been actively used for the preparation of commercial dairy products in Russia for almost 40 years. It was demonstrated that this strain possesses high activities of β-galactosidase, α-glucosidase, and leucine arylaminidase; inhibits the growth of pathogens such as Salmonella typhimurium, Staphylococcus aureus, and Escherichia coli; and can efficiently remove cholesterol from the cultural medium. The resistance of B. longum MC-42 determined for 15 commonly used antibiotics was in agreement with those previously reported for Bifidobacterium spp. The absence of frequently transmittable antibiotic resistance genes in the genome and the lack of undesirable activity of β-glucuronidase proved the safe use of B. longum MC-42 as a probiotic and starter culture. Additionally, the impact of two growth-promoting additives—yeast extract or milk protein hydrolysate containing supplementation—on the B. longum MC-42 fermentation profile was assessed. The introduction of these additives increases the maximum attainable viable cell count by orders of magnitude, significantly changed the profile of aminopeptidase activities in extracellular extracts, and influenced the antioxidant and antihypertensive properties of the obtained fermented products.

Survival of Salmonella dublin and Salmonella typhimurium in Lebanon Bologna

1975 ◽  
Vol 38 (3) ◽  
pp. 150-154 ◽  
Author(s):  
J. L. SMITH ◽  
S. A. PALUMBO ◽  
J. C. KISSINGER ◽  
C. N. HUHTANEN
Keyword(s):  
Salmonella Typhimurium ◽  
Starter Culture ◽  
Viable Cell ◽  
Salmonella Dublin ◽  
Aging Period ◽  
Cell Counts ◽  
Natural Flora

Survival and destruction of Salmonella dublin and S. typhimurium added to Lebanon bologna was studied during manufacture of this sausage. During the aging period of salted cubed beef at 5 C, viable cell counts of S. dublin did not change over a 10-day period. Cell counts of S. Dublin were reduced 3 to 4 log cycles during the 4-day fermentation at 35 C; further reduction in the viable Salmonella count occurred during mellowing of the bolognas at 5 C. The number of S. typhimurium was usually reduced to an undetectible level before the end of the fermentation. Salmonella typhimurium was consistently more sensitive to the acid conditions of Lebanon bologna than was S. dublin. Introduction of an optional cooking step indicated that heating of bolognas to 51.7 C or above led to destruction of salmonellae. Unaged beef which was not inoculated with starter culture did not ferment and there was very little reduction in numbers of added salmonellae. Salmonellae were destroyed more rapidly in Lebanon bologna made from unaged beef with starter culture than in bologna made from aged beef (natural flora fermentation). Smoking also appeared to contribute to destruction of salmonellae. Four commercial Lebanon bolognas were tested for the presence of salmonellae but none were detected.

scholarly journals Effect of Three Polysaccharides (Inulin, and Mucilage from Chia and Flax Seeds) on the Survival of Probiotic Bacteria Encapsulated by Spray Drying

2020 ◽  
Vol 10 (13) ◽  
pp. 4623
Author(s):  
Mariela Bustamante ◽  
Loreto Laurie-Martínez ◽  
Daniela Vergara ◽  
Rocio Campos-Vega ◽  
Mónica Rubilar ◽  
...  
Keyword(s):  
Spray Drying ◽  
Lactobacillus Casei ◽  
Bifidobacterium Longum ◽  
Storage Period ◽  
Probiotic Bacteria ◽  
Second Stage ◽  
Chia Seed ◽  
Flax Seeds ◽  
Face Centered ◽  
Central Composite

Chia seed mucilage (CM), flaxseed mucilage (FM), and inulin (INL) were used as encapsulating agents to evaluate the possibility of increasing the survival of Lactobacillus casei var. rhamnosus, renamed recently to Lacticaseibacillus rhamnosus, after spray drying. Moreover, the viability of encapsulated L. rhamnosus was determined during the 250 day storage period at 4 °C. In a second stage, the conditions that maximized the survival of L. rhamnosus were evaluated on other probiotic bacteria (Lactiplantibacillus plantarum, Bifidobacterium infantis, and Bifidobacterium longum). Additionally, the viability of encapsulated probiotics during the 60 day storage period at 4 and 25 °C was evaluated. The conditions that maximize the survival of L. rhamnosus (90%) predicted by a face-centered central composite design were 14.4% w/v of maltodextrin, 0.6% w/v of CM, and 90 °C of inlet air temperature. Additionally, under these encapsulating conditions, the survival of L. plantarum, B. infantis, and B. longum was 95%, 97%, and 96%, respectively. The probiotic viability improved during storage at 4 °C but decreased at 25 °C. The highest viability values obtained for probiotics during spray drying and during storage suggest a thermoprotector effect of CM, which would ensure an optimal probiotic efficacy in the product, thus promoting its utilization in the food industry.

scholarly journals Impact of some ingredients and the processing method on composition and quality of probiotic Labneh

2021 ◽  
Vol 26 (3) ◽  
pp. 2587-2593
Author(s):  
NABIL MEHANNA ◽  
◽  
SAMAR SALAMA ◽  
MOHAMED ARAFA
Keyword(s):  
Starter Culture ◽  
Milk Powder ◽  
Skim Milk ◽  
Storage Period ◽  
Total Solid ◽  
Skim Milk Powder ◽  
Probiotic Bacteria ◽  
Whey Protein Concentrate ◽  
Manufacturing Method

This study aims to produce probiotic Labneh by following the probiotic bacteria counts in the presence of yoghurt starter culture and some ingredients used in making Labneh with two different methods. Probiotic Labneh was prepared by the traditional method (TM) and direct formulation method (DFM). In both methods, used skim milk powder (SMP) for treatments (A and C), while used a mixture (1:1) of whey powder (WP) and whey protein concentrate (WPC) was used for treatments (B and D). The results indicated that total solid (TS), fat, protein and ash had the lowest values (P≤0.05) in B treatment, whereas the differences between the rest treatments were insignificant (P<0.05). Labneh made using the DFM had the lowest values for carbohydrate content and acidity and the highest pH values. The counts of Bifidobacteria and L. acidophilus were not influenced by the ingredients used or by the method applied for making the product. All fresh and stored Labneh had more than 6 log CFU/g for probiotic bacteria. Organoleptically, the general appearance, consistency and flavour of bio-Labneh were not significantly influenced by the applied treatments or by the manufacturing method but a gradual decrease in their scoring points were recorded with advancing storage period.

scholarly journals Electronic Nose for Monitoring Odor Changes of Lactobacillus Species during Milk Fermentation and Rapid Selection of Probiotic Candidates

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1539
Author(s):  
Zoltan Kovacs ◽  
Zsanett Bodor ◽  
John-Lewis Zinia Zaukuu ◽  
Timea Kaszab ◽  
George Bazar ◽  
...  
Keyword(s):  
Electronic Nose ◽  
Fermentation Process ◽  
Aroma Compounds ◽  
Probiotic Bacteria ◽  
Fermented Foods ◽  
Classification Models ◽  
Fermentation Time ◽  
Time Points ◽  
Cell Counts ◽  
Milk Fermentation

Probiotic bacteria have been associated with a unique production of aroma compounds in fermented foods but rapid methods for discriminating between foods containing probiotic, moderately probiotic, or non-probiotic bacteria remain aloof. An electronic nose (e-nose) is a high-sensitivity instrument capable of non-invasive volatile measurements of foods. In our study, we applied the e-nose to differentiate probiotic, moderately probiotic, and non-probiotic Lactobacillus bacteria strains at different fermentation time points (0th, 4th, and 11th) of milk fermentation. The pH of the changing milk medium was monitored with their corresponding increase in microbial cell counts. An e-nose with two gas chromatographic columns was used to develop classification models for the different bacteria groups and time points and to monitor the formation of the aromatic compounds during the fermentation process. Results of the e-nose showed good classification accuracy of the different bacteria groups at the 0th (74.44% for column 1 and 82.78% for column 2), the 4th (89.44% for column 1 and 92.22% for column 2), and the 11th (81.67% for column 1 and 81.67% for column 2) hour of fermentation. The loading vectors of the classification models showed the importance of some specific aroma compounds formed during the fermentation. Results show that aroma monitoring of the fermentation process with the e-nose is a promising and reliable analytical method for the rapid classification of bacteria strains according to their probiotic activity and for the monitoring of aroma changes during the fermentation process.

The impact of storage on the sensory, viscosity and chemical characteristics of yogurt fortified with oat β–glucan

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
X. Qu ◽  
Keyword(s):  
Storage Time ◽  
Dairy Product ◽  
Storage Period ◽  
Probiotic Bacteria ◽  
Experimental Sample ◽  
Ph Values ◽  
Normal Ranges ◽  
The Impact ◽  
Water Holding ◽  
Positive Effect

The feasibility of yogurt fortification with 0.3% oat β-glucan was examined. The results showed that 0.3% oat β-glucan yogurt has water-holding capacity. The acidity value and pH reached their maximum values at 7 d of storage, and no significant changes were observed after 7 d. All pH values ranged from 4.18 to 4.28, which are within the normal ranges for set-type yogurts. Interestingly, the viscosity values increased throughout storage. Significant differences were noted between the control yogurt and 0.3% oat β-glucan yogurt. The experimental sample had higher viscosity than the control yogurt, and the highest values were 58560 ± 2120 cp at 21 d for 0.3% oat β-glucan yogurt. The viability of probiotic bacteria in yogurts was checked. During the whole storage period, the content of probiotics decreased, which was only 0.63 ± 0.05×107 CFU/mL at 21 d. However, 0.3% oat β-glucan yogurt contained significantly more living probiotic bacteria compared to the control one, throughout the whole cold storage period. The viability of probiotic bacteria of 0.3% oat β-glucan yogurt at 14 d (3.18±0.2×107 CFU/mL) was only slightly lower than that of control yogurt at 1 d (3.45±0.3×107 CFU/mL). This fully demonstrates that the addition of 0.3% oat β-glucan has a protective effect on probiotics in yogurt, which will be beneficial for human health. The textural characteristics of yogurt were affected by the addition of 0.3% oat β-glucan, leading to decreased adhesiveness, but enhanced hardness and gumminess, throughout storage. All yogurts had average sensory scores of above 80, indicating a preference both for the control yogurt and 0.3% oat β-glucan yogurt throughout storage. The sensory results indicated that 0.3% oat β-glucan yogurt had the highest acceptability value of 86.49 at 21 d of storage, had a positive effect on the acceptability of the yogurt, independently of the storage time. Overall, yogurt containing 0.3% oat β-glucan could be an innovative healthy dairy product.

scholarly journals Viability of probiotic bacteria in bioyogurt with the addition of honey from Jataí and Africanized bees

2018 ◽  
Vol 53 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Luciana Albuquerque Caldeira ◽  
Érika Endo Alves ◽  
Antonia de Maria Filha Ribeiro ◽  
Vicente Ribeiro Rocha Júnior ◽  
Alciane Batista Antunes ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Starter Culture ◽  
Titratable Acidity ◽  
Fermented Milk ◽  
Probiotic Bacteria ◽  
Favorable Effect ◽  
Powdered Milk ◽  
Cell Counts ◽  
Randomized Design ◽  
Africanized Bees

Abstract: The objective of this work was to evaluate the viability of probiotic bacteria in bioyogurt with the addition of honey from Africanized and Jataí bees, in different concentrations. To prepare the fermented milk, reconstituted powdered milk and lactic acid starter culture were used. The bioyogurt was evaluated at 0, 7, 14, 21, 28, and 35 days of storage. Analyzes of pH, titratable acidity, and selective count of the Lactobacillus acidophilus LA-5 and Bifidobacterium BB-12 microorganisms were carried out. Counting was done, respectively, on MRS agar, in aerobiosis, and MRS-LP agar, in anaerobiosis, with plates incubated at 37°C for 72 hours. Treatments were arranged in a completely randomized design in split plot, with five treatments: without honey; 5 and 10% honey from Africanized bees, and 5 and 10% honey from Jataí bees. Storage times were evaluated in the split plots. In all treatments, bioyogurt showed counts of 107 CFU g-1 lactic acid bacteria. Probiotic cultures remained viable for 35 days under refrigeration (2-4°C). The interaction between the variation factors affected the probiotic concentration in the bioyogurt. The honeys have a favorable effect on the cell counts of the evaluated microorganisms.

Optimization of Mixed Fermentation Conditions by Mixed Culture of Kefir and Bacillus

2014 ◽  
Vol 618 ◽  
pp. 303-310
Author(s):  
Tie Hua Zhang ◽  
Xin Yu Zhang ◽  
Mu Tian ◽  
Shu Ning Zhong ◽  
Zhi Yang Du
Keyword(s):  
Monosodium Glutamate ◽  
Skim Milk ◽  
Viable Cell ◽  
Fermented Milk ◽  
Inoculum Size ◽  
Mixed Fermentation ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Cell Counts ◽  
Freeze Dried

Kefir is a kind of fermented milk that is produced by inoculating Kefir grains into milk, mainly consisting of bacteria and yeasts. Bacillus and their metabolites also have found some biotechnological applications in many aspects. The aim of the present study was to evaluate the mixed fermentation conditions by kefir and Bacillus. The results showed that kefir and Bacillus could be mixed fermentation, the suggested optimum fermentation conditions are the following: inoculum size was 4 mL of mixed fermentation agent for per 100 mL medium, the ratio to kefir and Bacillus is 2:1, fermentation temperature of 28 °C and fermentation time of 20 h. Under the optimum fermentation conditions, the viable cell counts of Lactic acid bacteria, yeast and Bacillus could above 1.0×1011, 4.0×1010, and 2.0×109CFU/mL respectively. After freeze-dried, by adding 10 % ( w/v ) skim milk, 3 % ( w/v ) glycerinum, 3 % trehalose and 1 % ( w/v ) Monosodium glutamate as for cryoprotectant, the viable cell counts could still above 107CFU/mL after 6 month.

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