Self-Organized Nanostructure Formation for Anti-Reflection Glass Surfaces

2012 ◽  
Vol 1389 ◽  
Author(s):  
Jörn Achtelik ◽  
Ricarda M. Kemper ◽  
Werner Sievers ◽  
Jörg K. N. Lindner
Keyword(s):  
Vapour Deposition ◽  
Normal Incidence ◽  
Nickel Surface ◽  
Force Microscopy ◽  
Self Organized ◽  
Atomic Force ◽  
Reflected Light ◽  
Glass Surfaces ◽  
Glass Microscope ◽  
Good Agreement

ABSTRACTTwo methods to create biomimetic anti-reflection nanostructures in ordinary glass microscope object slides are presented. One technique is based on a nanosphere lithography process combined with physical vapour deposition of nickel and reactive ion etching (RIE). The other uses plasma induced dewetting of a smooth nickel surface. The amount of reflected light was measured and a method to simulate the reflectivity from an atomic force microscopy (AFM) topography scan of the glass surface is presented. The reflectivity for visible light at normal incidence was reduced to 20-50 % of the original value with both methods and the simulation gives results in good agreement to the measurement.

scholarly journals 3D phonon microscopy with sub-micron axial-resolution

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Richard J. Smith ◽  
Fernando Pérez-Cota ◽  
Leonel Marques ◽  
Matt Clark
Keyword(s):  
Signal To Noise Ratio ◽  
Single Cells ◽  
Optical Resolution ◽  
Acquisition Time ◽  
Label Free ◽  
Axial Resolution ◽  
Force Microscopy ◽  
Atomic Force ◽  
Accuracy And Precision ◽  
Good Agreement

AbstractBrillouin light scattering (BLS) is an emerging method for cell imaging and characterisation. It allows elasticity-related contrast, optical resolution and label-free operation. Phonon microscopy detects BLS from laser generated coherent phonon fields to offer an attractive route for imaging since, at GHz frequencies, the phonon wavelength is sub-optical. Using phonon fields to image single cells is challenging as the signal to noise ratio and acquisition time are often poor. However, recent advances in the instrumentation have enabled imaging of fixed and living cells. This work presents the first experimental characterisation of phonon-based axial resolution provided by the response to a sharp edge. The obtained axial resolution is up to 10 times higher than that of the optical system used to take the measurements. Validation of the results are obtained with various polymer objects, which are in good agreement with those obtained using atomic force microscopy. Edge localisation, and hence profilometry, of a phantom boundary is measured with accuracy and precision of approximately 60 nm and 100 nm respectively. Finally, 3D imaging of fixed cells in culture medium is demonstrated.

scholarly journals Tunable control of antibody immobilization using electric field

2015 ◽  
Vol 112 (7) ◽  
pp. 1995-1999 ◽  
Author(s):  
Sam Emaminejad ◽  
Mehdi Javanmard ◽  
Chaitanya Gupta ◽  
Shuai Chang ◽  
Ronald W. Davis ◽  
...  
Keyword(s):  
Solid State ◽  
Electric Field ◽  
Signal To Noise Ratio ◽  
Physical Adsorption ◽  
Antibody Immobilization ◽  
Proof Of Concept ◽  
Force Microscopy ◽  
Atomic Force ◽  
Sensing Platforms ◽  
Glass Surfaces

The controlled immobilization of proteins on solid-state surfaces can play an important role in enhancing the sensitivity of both affinity-based biosensors and probe-free sensing platforms. Typical methods of controlling the orientation of probe proteins on a sensor surface involve surface chemistry-based techniques. Here, we present a method of tunably controlling the immobilization of proteins on a solid-state surface using electric field. We study the ability to orient molecules by immobilizing IgG molecules in microchannels while applying lateral fields. We use atomic force microscopy to both qualitatively and quantitatively study the orientation of antibodies on glass surfaces. We apply this ability for controlled orientation to enhance the performance of affinity-based assays. As a proof of concept, we use fluorescence detection to indirectly verify the modulation of the orientation of proteins bound to the surface. We studied the interaction of fluorescently tagged anti-IgG with surface immobilized IgG controlled by electric field. Our study demonstrates that the use of electric field can result in more than 100% enhancement in signal-to-noise ratio compared with normal physical adsorption.

scholarly journals AFM investigation of hyaline cartilage’s surface destruction

2016 ◽  
Author(s):  
Mikhail Ihnatouski ◽  
Dmitriy Karev ◽  
Boris Karev ◽  
Jolanta Pauk ◽  
Kristina Daunoravičienė
Keyword(s):  
Video Camera ◽  
Optical Microscope ◽  
Collagen Fibers ◽  
Force Microscopy ◽  
Digital Video Camera ◽  
Atomic Force ◽  
Reflected Light ◽  
Longitudinal Orientation ◽  
Chronic Progressive Disease ◽  
Surface Destruction

Introduction: Osteoarthritis is a chronic, progressive disease. The aim of this paper is presenting the AFM investigation of cartilage in relation to the assessment of degenerative changes in the surface of hyaline cartilage. It can be useful in choosing the most effective methods of therapy. Methods: Samples were taken from the cartilage surface of the femoral head after its removal during total hip arthroplasty. Images of the surface of the sample were obtained using an optical microscope equipped with a digital video camera, in the reflected light and by atomic force microscopy. Results: The longitudinal orientation of the collagen fibers and sub-fibers beams on the surface, up to a diameter of 50 nm are identified in non-destroyed area sites. Conclusions: Images of the destroyed areas displaying separately passing collagen fibers, strongly exposed to the surface: the size measured and found substructure.

Growth and characterization of InAs quantum dots on GaAs (100) emitting at 1.31μm.

2003 ◽  
Vol 794 ◽  
Author(s):  
V. Celibert ◽  
B. Salem ◽  
G. Guillot ◽  
C. Bru-Chevallier ◽  
L. Grenouillet ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Excitation Power ◽  
Good Growth ◽  
Excitation Power Density ◽  
Inas Quantum Dots ◽  
Force Microscopy ◽  
Self Organized ◽  
Gas Source ◽  
Atomic Force ◽  
Layer Deposition

ABSTRACTSelf-organized InAs quantum dots (QDs) were grown in the Stranski-Krastanov regime, by gas-source molecular beam epitaxy (GSMBE), on (100) GaAs substrates. Two important parameters have been optimized in order to grow high quality QDs with a very good reproducibility: InAs growth rate and GaAs cap layer deposition rate. Atomic force microscopy (AFM) analysis shows a unimodal QD distribution and the room temperature photoluminescence (RTPL) spectrum of the optimized sample reveals a 1.3 μm emission with a 19 meV full width at half maximum (FWHM). Photoluminescence (PL) measurements versus excitation power density and photoluminescence excitation (PLE) measurements clearly show multi-component PL emission from transitions associated with fundamental and related excited states of QDs. Furthermore a good growth reproducibility is observed. The results are promising for further work which will lead to laser fabrication.

Evaluation of the Strain Field Inside and Around Growth Islands by Means of X-Ray Diffuse Scattering

1999 ◽  
Vol 583 ◽  
Author(s):  
Martin Schmidbauer ◽  
Thomas Wiebach ◽  
Helmut Raidt ◽  
Peter Schäfer ◽  
Michael hanke ◽  
...  
Keyword(s):  
Strain Field ◽  
Reciprocal Space ◽  
X Ray Diffraction ◽  
Abrupt Increase ◽  
X Ray ◽  
Force Microscopy ◽  
Self Organized ◽  
Atomic Force ◽  
Transmission Electron ◽  
Highly Strained

AbstractThe strain distribution inside and in the vicinity of coherently strained self-organized islands has been investigated by high-resolution x-ray diffraction (HRXRD). Finite element method (FEM) calculations were carried out in order to calculate the strain field, which was then used to simulate x-ray reciprocal space maps on the basis of kinematical scattering theory. For Si0 75Ge0.25 islands an abrupt increase in the Ge-concentration at about one third of the island height has been found. This behavior can be attributed to different nucleation stages during growth. Highly strained buried CdSe quantum dots (QDs) strongly influence the surrounding ZnSe matrix. From reciprocal space maps and FEM simulations we were able to estimate the shape and size of the islands. The results are in agreement with transmission electron microscopy (TEM) and UHV atomic force microscopy (AFM) data.

Imaging the Composition of Oxide−Glass Surfaces by Friction Atomic Force Microscopy

Langmuir ◽  
2003 ◽  
Vol 19 (17) ◽  
pp. 6570-6572 ◽  
Author(s):  
Nathalie Destouches ◽  
Marie Foret ◽  
Eric Courtens ◽  
Michel Ramonda
Keyword(s):  
Atomic Force Microscopy ◽  
Oxide Glass ◽  
Force Microscopy ◽  
Atomic Force ◽  
Glass Surfaces

Atomic Force Microscopy and Transmission Electron Microscopy Study of Self-Organized Ordering in Vertically Aligned PbSe Quantum Dot Superlattices

2001 ◽  
Vol 696 ◽  
Author(s):  
A. Raab ◽  
G. Springholz ◽  
R. T. Lechner ◽  
I. Vavra ◽  
H. H. Kang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Atomic Force Microscopy ◽  
Quantum Dot ◽  
Force Microscopy ◽  
Self Organized ◽  
Atomic Force ◽  
Transmission Electron ◽  
Lateral Ordering ◽  
Spacer Thickness

AbstractSelf-organized lateral ordering is studied for PbSe/Pb1-xEuxTe quantum dot superlattices as a function of spacer thickness using atomic force microscopy and transmission electron microscopy. It is found that a pronounced hexagonal lateral ordering tendency exist not only for fcc-stacked superlattices but also for those with vertical dot alignment. For the latter case, a best in-plane ordering is observed for Pb1-xEuxTe spacer thicknesses around 160 Å. This is accompanied by a pronounced narrowing of the size distribution to values as low as ±8%. The resulting in-plane dot separations and dot densities are tunable by changes in spacer thickness. Similar marked changes are also found for PbSe dot shape as well as the dot sizes. This provides additional means for the tuning of the optical and electronic properties of the dots.

scholarly journals Atomic force microscopy of the morphology and mechanical behaviour of barnacle cyprid footprint proteins at the nanoscale

2009 ◽  
Vol 7 (43) ◽  
pp. 285-296 ◽  
Author(s):  
In Yee Phang ◽  
Nick Aldred ◽  
Xing Yi Ling ◽  
Jurriaan Huskens ◽  
Anthony S. Clare ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Sea Water ◽  
Persistence Length ◽  
Force Microscopy ◽  
Bond Model ◽  
Atomic Force ◽  
Extension Force ◽  
Force Curves ◽  
High Elongation ◽  
Glass Surfaces

Barnacles are a major biofouler of man-made underwater structures. Prior to settlement, cypris larvae explore surfaces by reversible attachment effected by a ‘temporary adhesive’. During this exploratory behaviour, cyprids deposit proteinaceous ‘footprints’ of a putatively adhesive material. In this study, footprints deposited by Balanus amphitrite cyprids were probed by atomic force microscopy (AFM) in artificial sea water (ASW) on silane-modified glass surfaces. AFM images obtained in air yielded better resolution than in ASW and revealed the fibrillar nature of the secretion, suggesting that the deposits were composed of single proteinaceous nanofibrils, or bundles of fibrils. The force curves generated in pull-off force experiments in sea water consisted of regions of gradually increasing force, separated by sharp drops in extension force manifesting a characteristic saw-tooth appearance. Following the relaxation of fibrils stretched to high strains, force–distance curves in reverse stretching experiments could be described by the entropic elasticity model of a polymer chain. When subjected to relaxation exceeding 500 ms, extended footprint proteins refolded, and again showed saw-tooth unfolding peaks in subsequent force cycles. Observed rupture and hysteresis behaviour were explained by the ‘sacrificial bond’ model. Longer durations of relaxation (>5 s) allowed more sacrificial bond reformation and contributed to enhanced energy dissipation (higher toughness). The persistence length for the protein chains ( L P ) was obtained. At high elongation, following repeated stretching up to increasing upper strain limits, footprint proteins detached at total stretched length of 10 µm.

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