Persistent Inhibition of Cell Growth on Silver Implanted Glassy Polymeric Carbon

ABSTRACTWe have shown that silver ion implantation or argon ion assisted surface deposition of silver inhibits cell growth on Glassy Polymeric Carbon (GPC), a desirable improvement of current cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that near surface implantation of silver in GPC can completely inhibit cell attachment on implanted areas while leaving adjacent areas vulnerable to strong cell adhesion. After cleaning and sterilization and more than one year in physiologic solution, the silver implanted GPC persists in inhibiting cell attachment.

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Patterning of Cell Attachment to Biocompatible Glassy Polymeric Carbon by Silver Ion Implantation

MRS Proceedings ◽

10.1557/proc-0942-w11-28 ◽

2006 ◽

Vol 942 ◽

Author(s):

Robert Lee Zimmerman ◽

Ismet Gurhan ◽

Daryush Ila ◽

F. Ozdal-Kurt ◽

B. H. Sen ◽

...

Keyword(s):

Ion Implantation ◽

Cell Attachment ◽

Blood Stream ◽

Tissue Growth ◽

Silver Ion ◽

Near Surface ◽

Precise Control ◽

In Vitro Biocompatibility ◽

Polymeric Carbon

ABSTRACTAlthough Glassy Polymeric Carbon (GPC) is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve. There is concern that the tissue lose adhesion and create the condition for embolisms downstream. We have shown that silver ion implantation or argon ion assisted surface deposition of silver inhibits cell growth on GPC, a desirable improvement of current cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that near surface implantation of silver in GPC can completely inhibit cell attachment on implanted areas while leaving adjacent areas unaffected. Patterned ion implantation permits precise control of tissue growth on medical applications of GPC.

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Enhanced Biocompatibility of GPC by Ion Implantation and Deposition

MRS Proceedings ◽

10.1557/proc-0908-oo16-04 ◽

2005 ◽

Vol 908 ◽

Author(s):

Robert Lee Zimmerman ◽

Ismet Gürhan ◽

Claudiu I. Muntele ◽

Daryush Ila ◽

Feyzan Özdal-Kurt ◽

...

Keyword(s):

Ion Implantation ◽

Biomedical Applications ◽

Heart Valves ◽

Cell Attachment ◽

Blood Stream ◽

Silver Deposition ◽

In Vitro Biocompatibility ◽

Artificial Heart Valves ◽

Model Cell

AbstractBiocompatible Glassy Polymeric Carbon (GPC) is used for artificial heart valves and in other biomedical applications. Although it is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve sometimes loses adhesion and creates embolisms downstream. Here we compare silver ion implantation and silver deposition, each of which strongly inhibits cell attachment on GPC. Inhibition of cell adhesion is a desirable improvement to current GPC cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that traces of silver can favorably influence the surface of GPC for biomedical applications.

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In vitro Biocompatibility Analyses of Stents Coated with Diamond-Like Carbon by Flow Cytometry, Cell Growth Assays and Electron Microscopy

Transfusion Medicine and Hemotherapy ◽

10.1159/000025268 ◽

2000 ◽

Vol 27 (4) ◽

pp. 200-206 ◽

Cited By ~ 1

Author(s):

K. Gutensohn ◽

C. Beythien ◽

R. Koester ◽

J. Bau ◽

T. Fenner ◽

...

Keyword(s):

Electron Microscopy ◽

Flow Cytometry ◽

Cell Growth ◽

Diamond Like Carbon ◽

In Vitro Biocompatibility

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Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe 3 O 4 nanoparticles for hBMSCs

Royal Society Open Science ◽

10.1098/rsos.172033 ◽

2018 ◽

Vol 5 (8) ◽

pp. 172033 ◽

Cited By ~ 6

Author(s):

Zhenfei Huang ◽

Zhihong Wu ◽

Bupeng Ma ◽

Lingjia Yu ◽

Yu He ◽

...

Keyword(s):

Cell Attachment ◽

Clinical Applications ◽

Bone Mesenchymal Stem Cells ◽

Water Contact ◽

Alizarin Red ◽

In Vitro Biocompatibility ◽

Related Transcription Factor ◽

Nanoparticle Coatings ◽

Bio Compatibility

Titanium (Ti) is an ideal bone substitute due to its superior bio-compatibility and remarkable corrosion resistance. However, in order to improve the osteoconduction and osteoinduction capacities in clinical applications, different kinds of surface modifications are typically applied to Ti alloys. In this study, we fabricated a tightly attached polydopamine-assisted Fe 3 O 4 nanoparticle coating on Ti with magnetic properties, aiming to improve the osteogenesis of the Ti substrates. The PDA-assisted Fe 3 O 4 nanoparticle coatings were characterized by scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy and water contact angle measurements. The cell attachment and proliferation rate of the human bone mesenchymal stem cells (hBMSCs) on the Ti surface significantly improved with the Fe 3 O 4 /PDA coating when compared with the pure Ti without a coating. Furthermore, the results of in vitro alkaline phosphatase (ALP) activity at 7 and 14 days and alizarin red S staining at 14 days showed that the Fe 3 O 4 /PDA coating on Ti promoted the osteogenic differentiation of hBMSCs. Moreover, hBMSCs co-cultured with the Fe 3 O 4 /PDA-coated Ti for approximately 14 days also exhibited a significantly higher mRNA expression level of ALP, osteocalcin and runt-related transcription factor-2 (RUNX2). Our in vitro results revealed that the present PDA-assisted Fe 3 O 4 nanoparticle surface coating is an innovative method for Ti surface modification and shows great potential for clinical applications.

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Synthesis, characterization and in vitro biocompatibility assessment of a novel tripeptide hydrogelator, as a promising scaffold for tissue engineering applications

Biomaterials Science ◽

10.1039/c6bm00287k ◽

2016 ◽

Vol 4 (10) ◽

pp. 1412-1416 ◽

Cited By ~ 4

Author(s):

Tihomir Pospišil ◽

Lejla Ferhatović Hamzić ◽

Lada Brkić Ahmed ◽

Marija Lovrić ◽

Srećko Gajović ◽

...

Keyword(s):

Tissue Engineering ◽

Cell Growth ◽

Growth Medium ◽

Engineering Applications ◽

In Vitro Biocompatibility ◽

Hydrogel Matrix

The supramolecular self-assembles of a simple tripeptide Ac-l-Phe-l-Phe-l-Ala-NH2form a hydrogel matrix which may serve as the cell growth medium.

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Size-Dependent in Vitro Biocompatibility and Uptake Process of Polymeric Carbon Nitride

ACS Applied Materials & Interfaces ◽

10.1021/acsami.9b17427 ◽

2019 ◽

Vol 11 (51) ◽

pp. 47739-47749 ◽

Cited By ~ 1

Author(s):

Malgorzata Aleksandrzak ◽

Magdalena Jedrzejczak-Silicka ◽

Krzysztof Sielicki ◽

Katarzyna Piotrowska ◽

Ewa Mijowska

Keyword(s):

Carbon Nitride ◽

Size Dependent ◽

In Vitro Biocompatibility ◽

Uptake Process ◽

Polymeric Carbon

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Synthesis, Surface and Cell-Adhesion Properties of Polyurethanes Containing Covalently Grafted RGD-Peptides

MRS Proceedings ◽

10.1557/proc-331-105 ◽

1993 ◽

Vol 331 ◽

Cited By ~ 2

Author(s):

Horng-Ban Lin ◽

Stuart L. Cooper

Keyword(s):

Cell Adhesion ◽

Endothelial Cell ◽

Cell Growth ◽

Culture Medium ◽

Cell Attachment ◽

Grafted Polymers ◽

Cell Adhesive ◽

Endothelial Cell Growth ◽

Endothelial Cell Adhesion

AbstractIn an attempt to improve endothelial cell adhesion and growth on a polyurethane copolymer, cell adhesive RGD-containing peptides were grafted to the polymer backbone. Two peptide grafting reaction schemes, including one-step and two-step approaches, were developed. Amino acid analysis confirmed that the two-step approach had a higher peptide coupling efficiency. The two-step reaction scheme was utilized to prepare GRGDSY, GRGDVY and GRGESY (inactive control) peptide grafted polyurethanes with two different peptide densities (100 and 250 μmol/g polymer). Dynamic contact angle measurements indicated that the surfaces of the peptide grafted polyurethanes were more hydrophilic than the starting and carboxylated versions of the precursor polyurethane. In-vitro endothelial cell adhesion experiments showed that, without the presence of serum in culture medium, the GRGDSY- and GRGDVY-grafted polyurethanes dramatically enhanced cell attachment and spreading. Increasing the peptide density from 100 to 250 μmol/g polymer for the GRGDSYand GRGDVY-grafted polyurethanes resulted in an increase in cell attachment. With approximately the same peptide density (100 or 250 μmol/g polymer), the GRGDVY-grafted polymers supported more adherent cells than the GRGDSY-grafted polymers. Similar trends were observed in the in-vitro endothelial cell growth studies using culture medium containing serum and endothelial cell growth supplement. These RGD-peptide grafted polyurethanes may be useful in providing an easily prepared cell-adhesive substrate for various implantable devices and hybrid organs.

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Patterned Adhesion of Cells

MRS Proceedings ◽

10.1557/proc-1020-gg05-01 ◽

2007 ◽

Vol 1020 ◽

Author(s):

Robert Lee Zimmerman ◽

Ismet Gurhan ◽

Daryush ILA

Keyword(s):

Cell Growth ◽

Cell Attachment ◽

Ion Beam ◽

Silver Ions ◽

Inhibitory Effect ◽

Tissue Growth ◽

Biocompatible Materials ◽

Silver Deposition

AbstractIt is well known that silver deposition avoids bacterial growth and inhibits the natural process of attachment of connective tissue to biocompatible materials in vivo. We have completed a five year investigation of the precise spatial control of cell growth on glassy polymeric carbon implanted with silver using ion beam techniques, and the persistence of the inhibitory effect on cell growth. Long term inhibition of cell growth on GPC is a desirable improvement on current cardiac implants and other biocompatible materials placed in the blood stream. We have used implanted silver ions near the surface of GPC to completely inhibit cell attachment and adhesion. Cells attach and strongly adhere to areas close to the silver implanted surfaces. Patterned ion implantation permits precise control of tissue growth on GPC and other biocompatible substrates. Cell growth limited to micrometric patterns on a substrate may be useful for in vitro studies of associated biological processes in an otherwise identical environment. The patterned inhibition of cell attachment persists for periods of time significant relative to typical implant lifetimes.

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Insights into the biochemical and functional characterization of sortase E transpeptidase of Corynebacterium glutamicum

Biochemical Journal ◽

10.1042/bcj20190812 ◽

2019 ◽

Vol 476 (24) ◽

pp. 3835-3847 ◽

Cited By ~ 1

Author(s):

Aliyath Susmitha ◽

Kesavan Madhavan Nampoothiri ◽

Harsha Bajaj

Keyword(s):

Protein Engineering ◽

Cell Attachment ◽

Functional Characterization ◽

Protein Structures ◽

Structural Similarity ◽

Surface Proteins ◽

Sortase A ◽

Peptide Cleavage ◽

New Findings

Most Gram-positive bacteria contain a membrane-bound transpeptidase known as sortase which covalently incorporates the surface proteins on to the cell wall. The sortase-displayed protein structures are involved in cell attachment, nutrient uptake and aerial hyphae formation. Among the six classes of sortase (A–F), sortase A of S. aureus is the well-characterized housekeeping enzyme considered as an ideal drug target and a valuable biochemical reagent for protein engineering. Similar to SrtA, class E sortase in GC rich bacteria plays a housekeeping role which is not studied extensively. However, C. glutamicum ATCC 13032, an industrially important organism known for amino acid production, carries a single putative sortase (NCgl2838) gene but neither in vitro peptide cleavage activity nor biochemical characterizations have been investigated. Here, we identified that the gene is having a sortase activity and analyzed its structural similarity with Cd-SrtF. The purified enzyme showed a greater affinity toward LAXTG substrate with a calculated KM of 12 ± 1 µM, one of the highest affinities reported for this class of enzyme. Moreover, site-directed mutation studies were carried to ascertain the structure functional relationship of Cg-SrtE and all these are new findings which will enable us to perceive exciting protein engineering applications with this class of enzyme from a non-pathogenic microbe.

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