Synthesis and Self-assembly of DMPC-conjugated Gold Nanoparticles

ABSTRACTBio-conjugated nanomaterials play a promising role in the development of novelsupramolecular structures, molecular machines, and biosensing devices. In this study, lipid-capped gold nanoparticles were synthesized and allowed to form a self-assembled monolayer structure. The nanoparticles were prepared by a phase transfer method, which involved the reduction of potassium tetrachloroaurate(III) by sodium citrate in an aqueous solution and the simultaneous transfer of the reduced species to an organic medium containing DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine). The gold nanoparticles were characterized using Uv-vis spectroscopy and dynamic light scattering (DLS) particle-size analysis. In addition, the resulting nanoparticles were examined using transmission electron microscopy (TEM). The Langmuir-Blodgett (LB) technique was used to assemble the DMPC-capped nanoparticles onto a water subphase at room temperature. The measurement of the compression isotherm confirmed the assemblage of lipid capped gold nanoparticles. This method of synthesis of ordered structures utilizing molecular interactions of lipids will be useful in developing novel metamaterials and nanocircuits.

Download Full-text

Biosynthesis of Gold Nanoparticles Using Fusarium oxysporum f. sp. cubense JT1, a Plant Pathogenic Fungus

ISRN Biotechnology ◽

10.5402/2013/515091 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 9

Author(s):

Janki N. Thakker ◽

Pranay Dalwadi ◽

Pinakin C. Dhandhukia

Keyword(s):

Antimicrobial Activity ◽

Gold Nanoparticles ◽

Chloride Solution ◽

Pathogenic Fungus ◽

Particle Size Analysis ◽

Size Analysis ◽

Plant Pathogenic Fungus ◽

Extracellular Synthesis ◽

Vis Spectroscopy ◽

Methods Of Synthesis

The development of reliable processes for the synthesis of gold nanoparticles is an important aspect of current nanotechnology research. Recently, reports are published on the extracellular as well as intracellular biosynthesis of gold nanoparticles using microorganisms. However, these methods of synthesis are rather slow. In present study, rapid and extracellular synthesis of gold nanoparticles using a plant pathogenic fungus F. oxysporum f. sp. cubense JT1 (FocJT1) is reported. Incubation of FocJT1 mycelium with auric chloride solution produces gold nanoparticles in 60 min. Gold nanoparticles were characterized by UV-Vis spectroscopy, FTIR, and particle size analysis. The particles synthesized were of 22 nm sized, capped by proteins, and posed antimicrobial activity against Pseudomonas sp.

Download Full-text

Phytochemical and Biological Evaluation of a Newly Designed Nutraceutical Self-Nanoemulsifying Self-Nanosuspension for Protection and Treatment of Cisplatin Induced Testicular Toxicity in Male Rats

Molecules ◽

10.3390/molecules26020408 ◽

2021 ◽

Vol 26 (2) ◽

pp. 408

Author(s):

Sherif R. Abdel-All ◽

Zeinab T. Abdel Shakour ◽

Dalia M. N. Abouhussein ◽

Enji Reda ◽

Thoraya F. Sallam ◽

...

Keyword(s):

Antineoplastic Agent ◽

Biological Evaluation ◽

Particle Size Analysis ◽

Male Rats ◽

Size Analysis ◽

Dilution Method ◽

Tribulus Terrestris ◽

Testicular Toxicity ◽

Transmission Electron ◽

Male Wistar Rats

The incorporation of cisplatin (CP) as a cytotoxic antineoplastic agent in most chemotherapeutic protocols is a challenge due to its toxic effect on testicular tissues. Natural compounds present a promising trend in research, so a new nutraceutical formulation (NCF) was designed to diminish CP spermatotoxicity. A combination of three nutraceutical materials, 250 mg Spirulina platensis powder (SP), 25 mg Tribulus terrestris L. extract (TT), and 100 mg fish oil (FO) were formulated in self-nanoemulsifying self-nanosuspension (SNESNS). SP was loaded into the optimized self-nanoemulsifying system (30% FO, 50% span 80/cremophor EL and 20% isopropanol) and mixed with TT aqueous solution to form SNESNS. For the SP, phytochemical profiling revealed the presence of valuable amounts of fatty acids (FAs), amino acids, flavonoids, polyphenols, vitamins, and minerals. Transmission electron microscopy (TEM) and particle size analysis confirmed the formation of nanoemulsion-based nanosuspension upon dilution. Method validation of the phytochemical constituents in NCF has been developed. Furthermore, NCF was biologically evaluated on male Wistar rats and revealed the improvement of spermatozoa, histopathological features, and biochemical markers over the CP and each ingredient group. Our findings suggest the potential of NCF with SNESNS as a delivery system against CP-induced testicular toxicity in male rats.

Download Full-text

Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics

Molecules ◽

10.3390/molecules26133790 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3790

Author(s):

Pratama Jujur Wibawa ◽

Muhammad Nur ◽

Mukhammad Asy’ari ◽

Wijanarka Wijanarka ◽

Heru Susanto ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Activated Carbon ◽

Aloe Vera ◽

Particle Size Analysis ◽

Size Analysis ◽

E Coli ◽

Transmission Electron ◽

Average Size ◽

Green Synthesized Silver Nanoparticles

This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

Download Full-text

Effects of Different Surfactant Charges on the Formation of Gold Nanoparticles by the LASiS Method

Materials ◽

10.3390/ma14112937 ◽

2021 ◽

Vol 14 (11) ◽

pp. 2937

Author(s):

Muhammad Zulfajri ◽

Wei-Jie Huang ◽

Genin-Gary Huang ◽

Hui-Fen Chen

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Gold Nanoparticles ◽

Laser System ◽

Synthesis Process ◽

Au Nps ◽

Laser Fluences ◽

Transmission Electron ◽

Vis Spectroscopy ◽

532 Nm

The laser ablation synthesis in solution (LASiS) method has been widely utilized due to its significant prospects in laser microprocessing of nanomaterials. In this study, the LASiS method with the addition of different surfactant charges (cationic CTAB, nonionic TX-100, and anionic SDS) was used to produce Au NPs. An Nd:YAG laser system at 532 nm excitation with some synthetic parameters, including different laser fluences, ablation times, and surfactant concentrations was performed. The obtained Au NPs were characterized by UV-Vis spectroscopy, transmission electron microscopy, and zeta potential analyzer. The Au NPs exhibited the maximum absorption peak at around 520 nm for all samples. The color of Au NPs was changed from red to reddish by increasing the laser fluence. The surfactant charges also played different roles in the Au NPs’ growth during the synthesis process. The average sizes of Au NPs were found to be 8.5 nm, 5.5 nm, and 15.5 nm with the medium containing CTAB, TX-100, and SDS, respectively. Besides, the different surfactant charges induced different performances to protect Au NPs from agglomeration. Overall, the SDS and CTAB surfactants exhibited higher stability of the Au NPs compared to the Au NPs with TX-100 surfactant.

Download Full-text

Fullerene-Gold Core-Shell Structures and Their Self-Assemblies

International Journal of Nanoscience ◽

10.1142/s0219581x16500290 ◽

2017 ◽

Vol 16 (02) ◽

pp. 1650029

Author(s):

R. Sepahvand ◽

S. Alihosseini ◽

M. Adeli ◽

P. Sasanpour

Keyword(s):

Citric Acid ◽

Self Assembly ◽

Size Structure ◽

Shell Structures ◽

Core Shell ◽

Hybrid Nanomaterial ◽

Gold Core ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Gold Nps

Hybrid nanomaterials consisting of functionalized fullerene and gold nanoparticle (NP) have been synthesized. Fullerene was functionalized by citric acid and used as reducing and capping agent for preparation of gold NPs. Functionalization of fullerene by use of citric acid was performed by enzymatic and thermal approaches. The core-shell structures containing gold NPs as core and fullerene as shell (gold/fullerene) were prepared. It was found that method and density of functionalization of fullerene effect final structure and therefore their physicochemical property of hybrid nanomaterial dramatically. Ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), atomic force microscopy (AFM), transmission electron microscopy (TEM) were used to analyze the structure of the synthesized hybrid nanomaterial and also study their self-assembly and physicochemical properties. Effect of the size, structure and morphology (e.g., agglomeration) of the synthesized hybrid nanomaterial on their UV-Vis absorption behavior has been also verified by theoretical modeling.

Download Full-text

Preparation of Amorphous Nanosilica from Philippine Waste Rice Hull via Acid Precipitation Method

Materials Science Forum ◽

10.4028/www.scientific.net/msf.864.112 ◽

2016 ◽

Vol 864 ◽

pp. 112-116

Author(s):

Rinlee Butch M. Cervera ◽

Emie A. Salamangkit-Mirasol

Keyword(s):

Electron Microscopy ◽

Low Cost ◽

Agricultural Waste ◽

Acid Precipitation ◽

Particle Size Analysis ◽

Precipitation Method ◽

Rice Hull ◽

Size Analysis ◽

Rice Hull Ash ◽

Transmission Electron

Rice hull or rice husk (RH) is an agricultural waste obtained from milling rice grains. Since RH has no commercial value and is difficult to use in agriculture, its volume is often reduced through open field burning which is an environmental hazard. In this study, amorphous nanosilica from Philippine waste RH was prepared via acid precipitation method. The synthesized samples were fully characterized for its microstructural properties. X-ray diffraction pattern reveals that the structure of the prepared sample is amorphous in nature while Fourier transform infrared spectrum showed the different vibration bands of the synthesized sample. Scanning electron microscopy (SEM) and particle size analysis (PSA) confirmed the presence of agglomerated silica particles. On the other hand, transmission electron microscopy (TEM) revealed an amorphous sample with grain sizes of about 5 to 20 nanometer range and has about 95 % purity according to EDS analyses. The elemental mapping also suggests that leaching of rice hull ash effectively removed the metallic impurity such as potassium element in the material. Hence, amorphous nanosilica was successfully prepared via a low-cost acid precipitation method from Philippine waste rice hull.

Download Full-text

Aggregation of Gold Nanoparticles in Presence of the Thermoresponsive Cationic Diblock Copolymer PNIPAAM48-b-PAMPTMA6

Polymers ◽

10.3390/polym13234066 ◽

2021 ◽

Vol 13 (23) ◽

pp. 4066

Author(s):

David Herrera Robalino ◽

María del Mar Durán del Amor ◽

Carmen María Almagro Gómez ◽

José Ginés Hernández Cifre

Keyword(s):

Gold Nanoparticles ◽

High Ionic Strength ◽

Solution Temperature ◽

Trimethylammonium Chloride ◽

Aggregation Process ◽

Critical Solution Temperature ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Copolymer Poly ◽

Positively Charged

The adsorption of the thermoresponsive positively charged copolymer poly(N-isopropylacrylamide)-block-poly(3-acrylamidopropyl)trimethylammonium chloride, PNIPAAM48-b-PAMPTMA6(+), onto negatively charged gold nanoparticles can provide stability to the nanoparticles and make the emerging structure tunable by temperature. In this work, we characterize the nanocomposite formed by gold nanoparticles and copolymer chains and study the influence of the copolymer on the expected aggregation process that undergoes those nanoparticles at high ionic strength. We also determine the lower critical solution temperature (LCST) of the copolymer (around 42 °C) and evaluate the influence of the temperature on the nanocomposite. For those purposes, we use dynamic light scattering, UV-vis spectroscopy and transmission electron microscopy. At the working PNIPAAM48-b-PAMPTMA6(+) concentration, we observe the existence of copolymer structures that trap the gold nanoparticles and avoid the formation of nanoparticles aggregates. Finally, we discuss how these structures can be useful in catalysis and nanoparticles recovery.

Download Full-text

Biosynthesized gold nanoparticles-doped hydroxyapatite as antibacterial and antioxidant nanocomposite

Materials Research Express ◽

10.1088/2053-1591/ac3309 ◽

2021 ◽

Author(s):

Is Fatimah ◽

Putwi Widya Citradewi ◽

Amri Yahya ◽

Bambang Nugroho ◽

Habibi Hidayat ◽

...

Keyword(s):

Electron Microscopy ◽

Antioxidant Activity ◽

Particle Size ◽

Gold Nanoparticles ◽

Antibacterial Activity ◽

X Ray Diffraction ◽

Transmission Electron ◽

Vis Spectroscopy ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

Abstract The composite of green synthesized gold nanoparticles (Au NPs)-doped hydroxyapatite (HA) has been prepared. The gold nanoparticles were produced via bioreduction of HAuCl4 with Clitoria ternatea flower extract, and utilized in the synthesis of hydroxyapatite using Ca(OH)2 and ammonium diphosphate as precursor. The aim of this research is to study the structural analysis of the composite and antibacterial activity test toward Eschericia coli, Staphylococcus aureus, Klebsiela pneumoniae, and Streptococcus pyogenes. In addition, the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The monitoring of gold nanoparticles formation was conducted by UV–vis spectroscopy and particle size analyses, meanwhile the synthesized composite was studied using X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The results showed that homogeneously dispersed gold nanoparticles in HA structure was obtained with the particle size ranging at 5-80 nm. The nanocomposite demonstrated antibacterial activity against tested bacteria. The nanocomposite expressed an antioxidant activity as shown by the DPPH scavenging activity of 66 and 58% at the concentration of 100 μg/mL and 50 μg/mL, respectively.

Download Full-text

The Evaporative Decomposition of Solutions Method (Eds) for the Production of Ultrafine Y1Ba2Cu3O7 from Nitrate and Mixed Anion Precursor Solutions

MRS Proceedings ◽

10.1557/proc-169-385 ◽

1989 ◽

Vol 169 ◽

Cited By ~ 2

Author(s):

Rollin E. Lakis ◽

Sidney R. Butler

Keyword(s):

Electron Microscopy ◽

Particle Size ◽

Barium Carbonate ◽

Hollow Spheres ◽

Particle Size Analysis ◽

Size Analysis ◽

Gravimetric Analysis ◽

X Ray Diffraction ◽

X Ray ◽

Transmission Electron

AbstractY1Ba2Cu3O7 has been prepared by the evaporative decomposition of solutions method. Nitrate and mixed anion solutions were atomized and decomposed at temperatures ranging from 300°C to 950°C. The resulting materials have been characterized using x-ray powder diffraction, Thermal Gravimetric Analysis (TGA), particle size analysis, Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). The powder consists of 0.3 micron agglomerated hollow spheres with a primary particle size of 0.06 micron. TGA and x-ray diffraction indicate the presence of barium nitrate and barium carbonate due to incomplete decomposition and/or product contamination by the process environment.

Download Full-text

Functionalization of Gold Nanoparticles with Monosaccharide Mannose

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.01086 ◽

2021 ◽

pp. 6281-6284

Author(s):

Sambit Dash ◽

Pragna Rao ◽

Ullas Kamath ◽

Aparna R Pai ◽

Prasanna Kumar Reddy Gayam ◽

...

Keyword(s):

Gold Nanoparticles ◽

Phase Transfer ◽

Colour Change ◽

Cost Effective ◽

Biomedical Sciences ◽

Size Dependent ◽

Nanoparticles Dispersion ◽

Wide Range ◽

Three Stages ◽

Transfer Method

Gold nanoparticles have found a wide range of application in biomedical sciences. Unique properties of these metal nanoparticles include surface plasmon resonance and size dependent colour change. Various molecules have been functionalized on the gold nanoparticles surface but carbohydrates have garnered attention due to their properties and their role in living systems. However certain challenges make carbohydrate-gold nanoparticles association difficult to obtain and stabilize. This study was carried out to chemically remodel gold nanoparticles by adding a monosaccharide mannose to its surface. A modified phase transfer method was used to synthesize gold nanoparticles. The surface of the nanoparticles was fixed with cyanuric chloride to serve as a linker. Mannose was then linked to the linker molecule. All three stages of the process, gold nanoparticles, and gold nanoparticles with linker and gold nanoparticles with the carbohydrate were analyzed for size and stability. Zeta potential and UV-vis data exhibited stable gold nanoparticles dispersion, successful binding of linker molecule as well as the carbohydrate. This study shows a simple, cost-effective and robust method of glycomodification of gold nanoparticles surface which can further find use in wide ranging applications.

Download Full-text