scholarly journals INDUCED SPAWNING AND LARVAL DEVELOPMENT OF VIETNAMESE KOI, Anabas testudineus (Bloch, 1792), USING SALMON GONADOTROPIN RELEASING HORMONE ANALOGUE (S-GnRHA)

2021 ◽  
Vol 16 (1) ◽  
pp. 21
Author(s):  
Md. Imtiaz Rahman ◽  
Md. Sadiqul Islam ◽  
Md. Saddam Hossain ◽  
Md. Ripon Ali ◽  
Bipresh Das ◽  
...  

The present research work has been carried out to obtain evidence on breeding and embryonic growth of A. testudineus by using S-GnRHa. Fish were injected with three different doses (0.25, 0.5, and 1.0 mL/kg body weight) of synthetic hormone S-GnRHa each with three replications, where male brood fish received half of the doses of female. The fecundity of A. testudineus was ranged from 47,227 to 77,561 during the study period and except control group all the hormone received group ovulated within 12 hours of hormone injection. Among all groups, the highest fertilization rate (89.33%), highest hatching rate (79.5%) as well as highest survival rate (67.0%) was obtained at 0.5 mL/kg body weight hormone dose recipient group. The fertilized egg’s diameter was recorded as 77.59 ± 3.50 µm. The first cleavage had appeared within 18-25 min of fertilization and eventually the morula, blastula, and gastrula stages were observed at 3:10 h, 4 h, and 5:30 h, respectively after fertilization. Larvae with distinguished head, body form and tail appendage spotted between 17-22 h and the larvae started hatching at 19 h after fertilization. The average length of larvae accounted as 105.41 ± 3.73 µm. The findings of present study revealed that 0.5 mL/kg S-GnRHa could be efficient dose for successful induced breeding of A. testudineus.

2021 ◽  
Vol 8 (1) ◽  
pp. 171-179
Author(s):  
Md Zahid Hasan ◽  
Md Fakhrul Islam ◽  
Syed Ariful Haque ◽  
Md Saiful Islam ◽  
Md Mokhlasur Rahman ◽  
...  

An experiment on induced breeding of gang magur, Hemibagrus menoda (Hamilton, 1822) using Ovatide hormone was carried out at in the Field Laboratory Complex of Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. This study consists of three treatments each with three replications. The objective of the experiment was to find out the effective dose of Ovatide hormone for induced breeding. A total number of 54 brood fish were used for the experiment among which 36 were male and 18 were female. Brood fish were kept in the ratio of 2♂:1♀ for breeding purpose. Female brood fish were injected at the rate of 7, 5, 3 ml Ovatide/kg body weight while the males were injected with 3, 2.5 and 1.5 ml Ovatide/kg body weight respectively in T1, T2, and T3 at the same time. The brood fish were injected with single dose of Ovatide in all treatments. Ovulation rates were 0%, 100%, 63%; fertilization rates were 0%, 97%, and 90%, and hatching rates were 0%, 95% and 76% in treatments T1, T2, and T3 respectively. Fertilized eggs were incubated for 21-22 h in all the treatments. Higher ovulation rate (100 %), fertilization rate (97%), hatching rate (95%) and survival rate (85%) were found in treatment T2. Therefore, the optimum dose of Ovatide hormone for induced breeding of H. menoda is 2.5 and 5 ml Ovatide/kg body weight of male and female brood fish, respectively. Res. Agric., Livest. Fish.8(1): 171-179, April 2021


2016 ◽  
Vol 36 (9) ◽  
pp. 901-909 ◽  
Author(s):  
D Sheela ◽  
R Vijayaraghavan ◽  
S Senthilkumar

Buprenorphine drug cartridge was made for autoinjector device for use in emergency and critical situations to reduce the morbidity and mortality. Water-filled cartridges were prepared and buprenorphine was injected aseptically in the cartridge, to make 0.05 and 0.10 mg/mL. Rats were injected intraperitoneally, buprenorphine (0.3 and 0.6 mg/kg), repeatedly with the autoinjector and compared with manual injection (7 days and 14 days) using various haematological and biochemical parameters. No significant change was observed in the body weight, organ to body weight ratio and haematological variables in any of the experimental groups compared with the control group. Except serum urea and aspartate aminotransferase, no significant change was observed in glucose, cholesterol, triglycerides, bilirubin, protein, albumin, creatinine, uric acid, alanine aminotransferase, gamma glutamyltransferase and alkaline phosphatase. The autoinjectors deliver the drugs with spray effect and force for faster absorption. In the present study, the autoinjector meant for intramuscular injection was injected intraperitoneally in rats, and the drug was delivered with force on the vital organs. No significant difference was observed in the autoinjector group compared to the manual group showing tolerability and safety of the buphrenorphine autoinjector. This study shows that buprenorphine autoinjector can be considered for further research work.


2018 ◽  
Vol 13 (3) ◽  
pp. 213
Author(s):  
Jojo Subagja ◽  
Vitas Atmadi Prakoso

Ikan baung (Hemibagrus nemurus) memiliki prospek bagus untuk dikembangkan sebagai komoditas budidaya di Indonesia karena citarasa daging yang enak banyak diminati konsumen. Dewasa ini, peningkatan produksi benih dapat dilakukan melalui pemijahan buatan dengan manipulasi hormonal. Penelitian yang dilakukan bertujuan untuk mengevaluasi produksi induk ikan baung melalui pemijahan buatan dengan dosis penyuntikan GnRH-a berbeda. Penelitian dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Bogor pada bulan November 2017. Induk betina ikan baung yang digunakan berukuran 465,0 ± 71,8 g; dan induk jantan 426,3 ± 46,8 g. Induksi dilakukan dengan penyuntikan hormon GnRH-a dengan tiga dosis yang berbeda pada induk betina (0,3; 0,5; dan 0,7 mL/kg bobot badan), dan ikan jantan dengan dosis 0,4 mL/kg bobot badan. Masing-masing perlakuan terdiri atas tiga ekor induk betina sebagai ulangan. Hormon disuntikkan secara intramuskular, diberikan dua kali penyuntikan 35% dari dosis total pada penyuntikan pertama, dan 65% diberikan pada penyuntikan kedua, dengan interval waktu penyuntikan delapan jam. Parameter yang diamati yaitu jumlah telur ovulasi, derajat pembuahan, derajat penetasan, dan sintasan larva selama tujuh hari. Hasil penelitian menunjukkan bahwa dosis penyuntikan hormon GnRH-a 0,5 mL/kg pada induk ikan baung saat proses pemijahan buatan menghasilkan derajat penetasan yang lebih tinggi dibandingkan dosis 0,3 dan 0,7 mL/kg; serta sintasan larva yang lebih baik dibandingkan dosis 0,7 mL/kg. Sedangkan nilai jumlah telur yang berhasil ovulasi dan derajat pembuahan yang relatif lebih baik ditemukan pada perlakuan dosis 0,7 mL/kg dibandingkan dengan dosis 0,3 dan 0,5 mL/kg.Asian redtail catfish (Hemibagrus nemurus) has a good prospect to be developed as aquaculture commodity in Indonesia. It was proved by the demand of consumers regarding to its delicious flesh taste. Recently, efforts to increase seedling production could be implemented through artificial spawning with hormonal manipulation. This study was conducted to evaluate the production of broodstocks through artificial spawning with different doses of GnRH-a. Study was conducted at Research Institute for Freshwater Fisheries Germplasm, Cijeruk, Bogor in November, 2017. The broodstock used in this study was 465.0 ± 71.8 g for females, and 426.3 ± 46.8 g for males. Induction was performed by injection of GnRH-a hormone with three different doses on female broodstocks (0.3, 0.5 and 0.7 mL/kg of body weight), and male broodstocks with a dose of 0.4 mL/kg of body weight. Each treatment consisted of three females as replications. The hormone was injected intramuscularly, given twice injection: 35% of the total dose is given at the first injection, and the remaining 65% was given at the second injection, with an injection time interval of eight hours. The results showed the observed parameters of number of the ovulated eggs, fertilization rate, hatching rate, and larval survival for seven days, treatment dosage of 0.5 mL/kg showed better results (P<0.05) than the dosage of 0.3 mL/kg and 0.7 mL/kg. Parameters observed were number of ovulated eggs, fertility rate, hatching rate, and survival rate for seven days. The results showed that the dosage of 0.5 mL/kg GnRH-a injection to the broodstock during artificial spawning resulted in higher hatching rate compared to 0.3 and 0.7 mL/kg and better larval survival rate than the dosage of 0.7 mL/kg. Meanwhile, the number of ovulated eggs and fertility rate was relatively better in the treatment of 0.7 mL/kg compared with the dosage of 0.3 and 0.5 mL/kg.


2016 ◽  
Vol 1 (3) ◽  
pp. 607-611
Author(s):  
Md Ruhul Amin ◽  
Md Mamunur Rahman ◽  
Sopna Aktar ◽  
Md Badrul Alam ◽  
Md Ashraful Alam

The research work was conducted for induced breeding of Vietnames Koi (Anabas testudineus) using carpPG extract and Ovaprim hormone at Tasty Fish and Feeds Industries Limited, Melandah, Jamalpur during March to May, 2015. Brood fishes were reared for 4-5 months in the brood rearing pond by providing farm-made artificial feed (containing 32% protein) for maturation before onset of breeding season. Two different hormones i.e., PG-PG (both for male and female), and PG-Ovaprim (PG for male and Ovaprim for female) were used as inducing agents for the purpose of induction of breeding A. testudineus. The broods were induced with PG-PG hormone at the doses of 2 mg PG/Kg body weight of male and 7 mg PG/Kg body weight of female and PG-Ovaprim hormone were used at the doses of 2 mg PG/Kg body weight of male and 7 ml Ovaprim/Kg body weight of female and designated as treatments T1 and T2, respectively. Ovulation rates (100%) were similar in both the treatments, whereas, fertilization, hatching, and survival rates were 78.11±3.24%, 70.22±3.40%, and 51.22±3.96% in T1 and 71.00±3.97%, 60.89±4.20%, and 48.89±4.41% in T2, respectively. Although comparatively better breeding performance was found for carp PG extract but these values were not significantly higher compared to Ovaprim. Therefore, comparing the breeding performance and considering the cost-benefit analysis it is suggested that Ovaprim could be used as an alternative of carp PG extract for the induced breeding of Vietnames koi.Asian J. Med. Biol. Res. December 2015, 1(3): 607-611


Author(s):  
Ramesh Kumar ◽  
Sanjay Kumar ◽  
Jyoti Palod ◽  
Himani Tewari ◽  
Balwan Singh

The aim of the present study was to determine the effect of chedu and mattha on growth performance of Murrah buffalo calves. To assess the effect of chedu and mattha on growth rate, 20 growing buffalo calves of around 1 year of age and approximately of same weights were selected and were divided into 4 treatment groups having 5 animals in each group. The animals had access to ad libitum berseem hay as the basal roughage T0 (Control), T1 (Administration of mattha @ 2% of body weight with salt @ 0.5% of mattha), T2 (Application of chedu on their body @ 150 g per animal), T3 (Administration of mattha and application of chedu). Body weight of all buffalo calves was recorded at 15 days interval during the experimental period. From 75th day of experiment, significant effect (P<0.05) was observed in body weight, heart girth and height at withers of treatment groups as compared to control group whereas, body length and paunch girth showed significant effect (P<0.05) on 90th day of experiment. The results further indicated that use of chedu and mattha improved the health status of the calves and helped to attain a comparatively higher body weight and an early puberty. It can be concluded on the basis of findings of present research work that the use of chedu and mattha may be recommended for supplementation of calves’ diet, as it improves the health status of the calves and helps in the attainment of higher body weight and an early onset of puberty in the calves.


1970 ◽  
Vol 6 (2) ◽  
pp. 361-366
Author(s):  
MFA Molla ◽  
MR Amin ◽  
MN Sarowar ◽  
M Muhammadullah

The paper reports the first incidence of successful induction of breeding in the riverine catfish Rita rita using carp pituitary gland (PG) extract. A breeding trial using four PG doses viz. 80, 100, 120 and 140mg/kg body weight of fish was conducted to optimize the dose of pituitary gland (PG) extract in terms of induction of ovulation in female. The male received a dose of 40mg PG/kg body weight in all cases and was sacrificed for collection of milt. The best performance was shown by the fish treated with 100mg PG/kg body weight in respect of inducing ovulation in females and fertilization and hatching rates of eggs. Keywords: Induced breeding; Rita rita; Ovulation; Fertilization rate; Hatching rate DOI: 10.3329/jbau.v6i2.4835 J. Bangladesh Agril. Univ. 6(2): 361-366, 2008


2018 ◽  
Vol 13 (2) ◽  
pp. 115
Author(s):  
Lies Setijaningsih ◽  
Jojo Subagja ◽  
Deni Radona ◽  
Brata Pantjara ◽  
Anang Hari Kristanto ◽  
...  

Permintaan ikan belida cukup tinggi untuk memenuhi kebutuhan konsumsi dan bahan baku olahan makanan yang masih mengandalkan tangkapan dari alam. Tingginya eksploitasi mengakibatkan populasi ikan dari tahun ke tahun semakin berkurang dan terancam punah. Teknologi pembenihan ikan belida dengan penggunaan hormon diduga dapat meningkatkan produksi larva untuk keperluan budidaya. Penelitian dilakukan untuk mengevaluasi performasi pemijahan ikan belida secara alami dan buatan melalui induksi hormon HCG dan LHRH analog. Pemijahan alami dan buatan dilakukan menggunakan induk betina sebanyak enam ekor dan jantan tiga ekor (rasio 2:1). Induk yang digunakan berukuran panjang 46 ± 5 cm dan bobot 2.209 ± 623 g. Pemijahan buatan dilakukan dengan induksi hormon HCG dosis 500 IU/kg dan LHRH analog dosis 0,5 mL/kg. Penyuntikan HCG dan LHRH diberikan dengan interval 24 jam. Hasil penelitian menunjukkan pada pemijahan buatan (induksi hormon) diperoleh induk yang memijah sebanyak dua ekor dengan nilai diameter telur (3,1 ± 0,3 mm); fekunditas (282-907 butir); derajat pembuahan (21%-40%); derajat penetasan (56%-75%); dan sintasan (30%-50%); sedangkan pada pemijahan alami diperoleh satu ekor induk yang memijah dengan nilai diameter telur (3,5 ± 0,3 mm); fekunditas (1.616 butir); dan derajat pembuahan (86,7%); selama tiga bulan pengamatan secara intensif dengan frekuensi pemijahan enam kali. Aplikasi teknologi pemijahan dengan menggunakan induksi hormon dapat menghasilkan produk larva pada ikan belida.The demand for fish consumption of Clown Knifefish is considerably high. However, the fish supply for consumption and processed food still relies on wild-caught. Such exploitation has decreased the population of Clown Knifefish to a point that it can become an endangered species. Efforts on the breeding technology of Clown Knifefish through natural and artificial propagation using of hormones have shown promising results. The current study was conducted to evaluate the performance of Clown Knifefish in natural and artificial spawning conditions using the induction of HCG hormone and LHRH analogues. The natural and artificial spawning treatments were conducted using six fish females and three fish males (a ratio of 2:1). The average Broodstocks’ size was 46 ± 5 cm in length and 2,209 ± 623 g in weight. The artificial spawning was conducted with HCG hormone induction (dose of 500 IU/kg body weight) and LHRH analogue (dose: 0.5 mL/kg). HCG and LHRH injections were given within 24 hours interval. The results showed that the artificial spawning (hormone induction) had successfully caused two broodstock to spawn producing eggs with the diameter of 3.1 ± 0.3 mm; fecundity of 282-907 eggs; fertilization rate of 21%-40%; hatching rate of 56%-75%; and survival rate of 30%-50%. In the natural spawning, one broodstock had spawned with the egg diameter of 3.5 ± 0.3 mm; fecundity of 1,616 eggs; and fertilization rate of 86.7%. This study concludes that spawning technology applications using hormonal induction can increase the production larvae of Clown Knifefish broodstock.


2012 ◽  
Vol 9 (2) ◽  
pp. 305-310
Author(s):  
MM Rahman ◽  
MI Miah ◽  
KR Hasan ◽  
SM Farid

Artificial propagation of Mastacembelus pancalus was conducted in order to determine the optimum dose of pituitary  gland (PG) hormone at the hatchery of the Field Laboratory Complex, Faculty of Fisheries, Bangladesh Agricultural  University, Mymensingh. The present study consisted of two trials and each trial had four treatments (T1, T2, T3 and  T4, respectively) with three replications of each. In the experiment single dose and double dose had been used for T1,  T2, T3 and T4, respectively to evaluate the efficiency on ovulation rate, fertilization rate, and hatching rate of M.  pancalus eggs. The hatchlings were reared in aquarium up to 30 days and survival rate was determined. Treatment-3  (T3) of double dose (170mg PG kg-1 and 60mg PG kg-1 body weight of fish for female and male respectively) showed  better results in terms of ovulation rate (90.03±2.56%), fertilization rate (90±0.81%), hatching rate (80±1.84%), and  survival rate (40.00±3.19%) was recorded than other treatments of single and double dose. The water temperature  was recorded 27 to 31°C. In the present study, treatment-3 (T3 in double dose) was the best findings in terms of  ovulation rate, fertilization rate, and hatching rate which can be used in artificial propagation of M. pancalus for the  development of hatchery production. May and June are the suitable months for artificial propagation of the said  species.   DOI: http://dx.doi.org/10.3329/jbau.v9i2.11045   J. Bangladesh Agril. Univ. 9(2): 305–310, 2011


Author(s):  
S Hayat ◽  
M Ramzan ◽  
M Zafarullaf ◽  
I Ahmad ◽  
Q Ali ◽  
...  

In present study the Effects of different doses of ovaprim on induced spawning activities of Rita rita were observed with an aim to standardize the dose of ovaprim for successful breeding. In the trial experiment, the females of Rita rita were treated with ovaprim at the rate of 0.5, 0.8 and 1.0ml/kg body weight and males of Rita rita in all the cases were treated at the rate of 0.4ml/kg.  The doses of ovaprim at the rates of 0.5, 0.8, 1.0 ml/kg body weight resulted in 0%, 100% and 100% ovulation respectively. In case of injection of ovaprim at the rate of 0.8ml/kg, the fertilization rate was observed to be about 70% and hatching rate was 58%, which occurred within 20-25 hours after fertilization at water temperature of 270C-290C. In case of injection of ovaprim at the rate of 1.0ml/kg, 18% fertilization rate and 5% hatching rate was observed. The doses of ovaprim at the rates of 0.8ml/kg and 0.4ml/kg body weight for female and male Rita rita respectively was found to be satisfactory for overall breeding performance and the commercially successful production of this fish. Hatchlings from over all experiment were reared up to fingerlings size to about 2 month and were finally stocked into the Chashma lake.


2017 ◽  
Vol 14 (2) ◽  
pp. 191-197
Author(s):  
M. Ahammed ◽  
M. A. Ali ◽  
M. A. Ehsan ◽  
M. Mostafa

The experiment was carried out to determine the efficacy of Ivermectin (Ivomec® S/C Formulation) against gastrointestinal nematodiasis and ectoparasites including stephanofilaria on crossbred cattle, to determine the effect of drug on certain hematological parameters like hemoglobin (Hb) content, packed cell volume (PCV) and erythrocyte sedimentation rate (ESR)in crossbred cattle and to determine the effect of the drug on live weight. A total of 100 cattle were selected randomly and examined for presence of both endo and ectoparasites including stephanofilaria. Gastrointestinal nematodiasis were detected by examination of fecal samples and ectoparasites as well as stephanofilaria were detected by physical examination. Out of 100 cattle, 20 were found to suffer from both endo and ectoparasites. These 20 cattle were selected finally for the research work. Then these cattle were divided into two groups, group A (treated group, n=15) and group B (control group, n=5). Ivermectin was injected subcutaneously to the cattle of group A @ 200µm/kg body weight (1ml/50 kg body weight). The therapeutic efficacy of the drug against gastrointestinal nematodiasis was determined by investigation of fecal egg count reduction and the efficacy was 100% against the common nematodes in crossbred cattle on day 7, 14, 21 and 28 of post treatment period. Ticks within a markable area (25 square inches) were counted on day 0 and lice infestation was marked as infected on day 0. Ivermectin showed 100% effectiveness at the 7, 14, 21 and 28 day of post treatment period against both tick and lice infestation. The efficacy of the drug against stephanofilariasis (humpsore) was determined by reduction of diameter of the sore on the day 14, 42 and 56 of post treatment period. All the sores were completely healed up by the 56 days. During the study of hematological parameters it was seen that Hb and PCV were increased whereas ESR values were decreased on post treatment days. In this study the mean live weight of the cattle of treated group was increased after treatment with ivermectin and increased body weight was 5.13% on the 28th day of post treatment period. From the above findings it may be concluded that ivermectin was 100% effective against gastrointestinal nematodes common in crossbred cattle. Effectiveness of the drug against external parasites was 100%. In this study hematological parameters were changed significantly after treatment with ivermectin, body weight of animal treated with ivermectin was increased and no adverse effect of the drug was found in this study.


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