scholarly journals Research of the colonization level of the fungi Malassezia spp. and Candida spp. on the skin of patients with skin diseases

2018 ◽  
Vol 0 (1 (21)) ◽  
pp. 29-32
Author(s):  
Tetiana Chastii
Keyword(s):  
Skin Diseases ◽  
Candida Spp ◽  
Malassezia Spp

scholarly journals Micoses superficiais na cidade de Manaus, AM, entre março e novembro/2003

2006 ◽  
Vol 81 (3) ◽  
pp. 238-243 ◽  
Author(s):  
José Augusto Almendros de Oliveira ◽  
Jacqueline de Aguiar Barros ◽  
Ana Cláudia Alves Cortez ◽  
Juliana Sarmento Rocha Leal de Oliveira
Keyword(s):  
Tinea Capitis ◽  
Candida Spp ◽  
Malassezia Spp

FUNDAMENTOS: Micoses superficiais estritas são infecções fúngicas que se localizam nas camadas superficiais da pele e seus anexos. As micoses superficiais cutâneas representadas pelas dermatofitoses e candidíases podem ultrapassar a camada córnea da pele. Na região amazônica possuem incidência elevada. OBJETIVOS: Estudar as micoses superficiais, estritas e cutâneas, diagnosticadas sob o ponto de vista epidemiológico e micológico. PACIENTES E MÉTODOS: Pacientes com suspeita clínica de micoses superficiais submetidos a exame micológico no período de março a novembro de 2003 no Laboratório de Micologia Médica/CPCS/INPA. RESULTADOS: Foram realizados 394 exames, tendo 256 apresentado diagnóstico positivo. As micoses mais incidentes foram onicomicoses (135) e pitiríase versicolor (98). Malassezia spp. (77) e Candida spp. (72) foram os agentes fúngicos mais isolados. Tinea capitis apresentou maior ocorrência nos pré-escolares (3), e onicomicoses em adultos (94). O sexo feminino foi o mais acometido (91). Todas as classes sociais foram infectadas, com predominância da C (37). CONCLUSÃO: Onicomicoses e pitiríase versicolor acometeram sobretudo adultos. A Tinea capitis ocorre principalmente, em crianças. As micoses superficiais apresentaram mais incidentes nas mulheres. Malassezia spp. e Candida spp. foram os agentes mais isolados.

Accuracy of Interpretation of Fungi by Direct Microscopy using Chlorazol Black E versus Gold Standard Potassium Hydroxide

2021 ◽  
Vol 104 (3) ◽  
pp. 383-387
Keyword(s):  
Microscopic Examination ◽  
Potassium Hydroxide ◽  
Skin Diseases ◽  
Fungal Infections ◽  
Candida Spp ◽  
Direct Microscopy ◽  
Direct Microscopic Examination ◽  
Negative Findings ◽  
Siriraj Hospital ◽  
The Mean

Background: Superficial fungal infections are skin diseases that affect people worldwide. To confirm the diagnosis of these diseases, mycological investigation by direct microscopic examination and culture are required. Chlorazol black E (CBE) is an acid-based dye of the trisazo group of dyes that has a high affinity for chitin. Data specific to the efficacy of CBE for detection of fungi by direct microscopy is limited. Objective: To investigate the accuracy of interpretation of fungi by direct microscopy using CBE versus potassium hydroxide (KOH). Materials and Methods: The present study was a retrospective study, conducted at the Department of Dermatology, Faculty of Medicine, Siriraj Hospital, Mahidol University in 2018. Slide interpretations by 33 microscopists were reviewed. The interpretation scores of all participants in each specimen were recorded and compared between CBE and KOH. Results: Thirty-three participants were included. The mean interpretation score of all participants was 17.2±4.9. The mean interpretation scores of overall specimens using CBE was significantly more than using KOH (p<0.001). For Candida spp. and dermatophytes, the accuracy of interpretation using CBE was statistically higher than KOH (p=0.020, p<0.001, respectively). Relative to negative findings, CBE yielded significantly more false-positive results than KOH (p=0.003). Conclusion: CBE for direct microscopic examination of fungi specimens is helpful for increasing the accuracy of interpretation. However, falsepositive results are more prevalent when using CBE. Keywords: Accuracy, Interpretation, Fungus, Direct microscopy, Chlorazol black E, Potassium hydroxide

scholarly journals A study on malassezia micro flora in the skin of healthy individuals in North Kerala, India

2017 ◽  
Vol 5 (10) ◽  
pp. 4600 ◽  
Author(s):  
Remya V. S. ◽  
Arun B. ◽  
Sheeba P. M. ◽  
Pratyusha Kokkayil
Keyword(s):  
Recovery Rate ◽  
Skin Diseases ◽  
Common Species ◽  
Species Variation ◽  
Healthy Individuals ◽  
Gender And Age ◽  
Significant Difference ◽  
The Common ◽  
Malassezia Spp ◽  
Apparently Healthy

Background: Malassezia spp are accounted for approximately 60-90% of the total cutaneous fungal micro biota. An enhanced understanding of the distribution of Malassezia spp in healthy individuals is necessary to gain insight to involvement of these species in human skin disorders particularly in pityriasis versicolor. The aim of this study was to assess the Malassezia micro flora of healthy individuals and to identify species variation with different body sites, gender and age groups.Methods: A total of 120 apparently healthy men and 120 women without any skin diseases or who were not treated for Malassezia associated skin diseases were selected for the study. The specimens consisted of scrapings collected from chest, back and neck from each control subject totaling 720 sites.Results: The recovery rate of Malassezia was positive in case of 452 (62.78%) of 720 sites sampled, while the remaining 268 (37.22%) were found to be negative. Recovery rate of Malassezia was highest in chest with 83.33% positive cases, followed by the back with 73.33% and the neck 31.67%. M. globosa was identified as the common species (46.46%) followed by M. furfur (25%) and M. restricta (22.56%). No significant difference was present (P > 0.05) in distribution of species with different body sites, gender and age groups.Conclusions: The overall colonization rate of Malassezia spp in normal healthy individuals found in this study was 62.78%. M. globosa was identified as the common species (46.46%).

scholarly journals The first case of isolation of malassezia globosa in our country

2003 ◽  
Vol 131 (11-12) ◽  
pp. 454-457 ◽  
Author(s):  
Valentina Arsic-Arsenijevic ◽  
Danica Milobratovic ◽  
Aleksandar Dzamic ◽  
Sanja Mitrovic ◽  
Ivana Radonjic ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Laboratory Diagnosis ◽  
Pityriasis Versicolor ◽  
Isolation And Identification ◽  
First Case ◽  
Skin Flora ◽  
Malassezia Globosa ◽  
Malassezia Spp ◽  
Systemic Infections

Today is known that genus Malassezia includes seven species: M. furfur, M. sympodialis, M. obtusa, M. globosa, M. restricta, M. slooffiae and M. pachydermatis, but role of each of the species in the pathogenesis of desease has not been eluciated yet, so futher laboratory isolation and identification are necessary. We report the first case of isolation of Malassezia globosa in Serbia (Belgrade), in a patient suffering from Pityriasis versicolor. Identification of M. globosa was based on macroscopic, microscopic and biochemical characteristics. Isolation was done on Leeming and Notman medium and on mDixona agar, at 350C, during 7 days in aerobic conditions. Also the yeast's biochemical phenotype was determined as catalase (+), lipase (+), esculin degradation (-), Tween (20,40, 60 and 80) asimilation (-). M. globosa is a lipophilic yeast of the genus Malassezia and the common member of the skin flora. In concordance with some predis-poning factors M. globosa is implicated in the pathogenesis of several skin diseases (pityriasis versicolor, malassezia foliculitis sebor-rheic dermatitis and some forms of atopic dermatitis). In immuno-compromised patients and neonates this yeast can even cause fatal systemic infections. Because the role of Malassezia spp. in pathogenesis of skin desease is not still determined, we suggest laboratory diagnosis and identification of these species as a routine diagnostic procedure.

scholarly journals Determination of the activity of fungicides against pathogens of dermatomycoses in domestic animals

2020 ◽  
Vol 22 (99) ◽  
pp. 20-23
Author(s):  
I. M. Kushnir ◽  
V. I. Kushnir ◽  
B. V. Gutyj ◽  
I. S. Semen ◽  
S. D. Murska ◽  
...  
Keyword(s):  
Pathogenic Fungi ◽  
Laboratory Diagnosis ◽  
Domestic Animals ◽  
Chlorhexidine Digluconate ◽  
Microscopic Fungi ◽  
Fusarium Spp ◽  
Candida Spp ◽  
Opportunistic Fungi ◽  
Highly Active ◽  
Malassezia Spp

The aim of the study was to identify and identify the causative agents of dermatomycosis in dogs and cats and to determine their sensitivity to solutions of ketonazole and chlorhexidine digluconate, which are part of the drug Skinhard. This is a spray for external use, manufactured by PJSC “Halychpharm”. For laboratory diagnosis of dermatomycoses, samples from the affected parts of the skin and fur of domestic animals were used. To isolate a pure culture of fungi were sown on selective media: wort agar, agar Saburo, Chapek. The optimal cultivation regime for pathogenic fungi was 20–25 °С. Isolated fungal cultures were identified by the appearance and shape of colonies, their consistency, color, ability to grow at 37 °C, microscopic structure, in particular - the nature of mycelial branching and the presence of septa, location of conidiophores, spores and other signs. A microbiological examination of the affected areas of the skin of dogs and cats was performed to detect microscopic fungi. The following fungi were isolated from dogs affected by mycoses: Candida spp., Aspergillus niger, Epidermophyton spp., Microsporum spp., Mucor spp., Trichophyton spp., Mallasseria spp., Sporotrich spp, Candida albicans, and from cats: Microspor Candida spp., Spototrich spp., Rhizorus spp., Fusarium spp. Trichophyton spp. Isolated microscopic fungi of the genus Malassezia spp. belong to superficial mycoses (keratomycoses) and affect the superficial layers of skin and hair. Epidermatophytes: Trichophyton spp., Epidermophyton spp., Microsporum spp. affect the epidermis, skin and coat. In addition, opportunistic fungi of the genus Aspergillus spp, Mucor spp., Rhizorus spp. and Fusarium spp. Chlorhexidine digluconate was found to be highly active against dermatophytes (Trichophyton spp., Microsporum spp.), Less active against fungi of the genus Candida and less active against Aspergillus spp, Mucor spp., Rhizorus spp, Fusarium spp. Ketonazole is highly active against dermatophytes (Trichophyton spp., Epidermophyton spp., Microsporum spp.), Yeast (Malassezia spp., Candida spp.,) And opportunistic fungi (Aspergillus spp., Mucor spp., Rhizorus spp.).

scholarly journals Antifungal Activity of the Essential Oils of Osteophloeum platyspermum (Myristicaceae) against Malassezia spp. and Candida albicans Influenced by Seasonality and Climatic Factors

2021 ◽  
pp. 31-45
Author(s):  
Jefferson S. Silva ◽  
Victoria R. Brandão ◽  
Selene D. A. Coutinho ◽  
Mateus L. B. Paciencia ◽  
Sergio A. Frana ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Skin Diseases ◽  
Multivariate Analyses ◽  
Climatic Factors ◽  
New Drugs ◽  
Microbial Pathogens ◽  
Minimal Bactericidal Concentration ◽  
Wide Range ◽  
Malassezia Spp

Aims: Malassezia spp. are involved in a wide range of mammalian skin diseases. The introduction of new drugs is a need. Natural products are known to be effective in the treatment of microbial pathogens. The present study analyzed the O. platyspermum leaf essential oils (EOs) antifungal activity. Study Design: 18 terpenes from 13 O. platyspermum leaf EOs are related to seasonal and climatic variations occurring during the dry (DS) and rainy (RS) seasons in the Amazon Rain-Forest, verified by means of multivariate analyses. Place and Duration of the Study: the study was conducted at the Center for Research in Biodiversity (Microbiology Laboratory and Cell Culture Laboratory), Paulista University, biological activity evaluations occurred between January/2019 and December 2019). Methodology: microdilution broth assay was used in the minimal bactericidal concentration (MBC). Multivariate analyses were used to access the relationship among MBC, seasonality and terpene composition of the EOs. Results: Malassezia pachydermatis showed higher sensitivity to the EOs than M. furfur or C. albicans. The DS EOs were linked to the presence of limonene, myrcene, α-terpineol, linalool, terpinen-4-ol, cubenol-1-epi, influenced by insolation, temperature and evaporation, while β-elemene, γ-elemene, neo-intermedeol, elemol, α-cadinol, spathulenol, isospathulenol, viridiflorol, δ-amorphene and ledol were linked to the RS EOs, and were influenced by precipitation, relative humidity and wind velocity. DS EOs showed better antifungal activity against both Malassezia species, and the presence of the six discriminative terpenes was essential for the antifungal activity. Conclusions: The DS EOs are a potential source of new leads to defeat animal dermatological microbes.

scholarly journals ​Evaluation of Fungi Isolated in the Veterinary Microbiology Laboratory in Terms of Human Health

2021 ◽  
Author(s):  
S. Kahya Demirbilek ◽  
Ö. Ardıçlı ◽  
H. Kurnaz
Keyword(s):  
Animal Species ◽  
Fungal Infections ◽  
Significant Risk ◽  
Microbiology Laboratory ◽  
Candida Spp ◽  
Animal Products ◽  
Pet Owners ◽  
Direct Microscopic Examination ◽  
Malassezia Spp ◽  
Penicillium Spp

Background: The importance of zoonotic fungal infections is increasing today and continues to increase due to changing living conditions. Methods: Between 2012 and 2019 evaluated of fungi species isolated from 51 (20.07%) of 254 samples from 10 different animal species (dog, cat, horse, cow, goat, sheep, camel, penguin, bird, rabbit) with 10 different sample types Thus, the risk of those with zoonotic characteristics was assessed. Despite fungal hyphae and/or spores were seen in 63 (24.8%) of 254 samples in the direct microscopic examination, the isolation of fungi on Sabouraud dextrose agar occurred in only 51 of these samples (20.07%). Conclusion: Of all the 51 samples, 9 (17.64%) samples had more than one fungal agent. The predominant isolate was Aspergillus spp. with an isolation rate of 24 (47.05%), followed by Malassezia spp. 11 (21.56%), Alternaria spp. 6 (11.76%), Penicillium spp. and various yeasts 4 (7.84%), Microsporum spp. 3 (5.88%), Candida spp., Mucor spp., Geotrichum spp. 2 (3.92%) and Trichophyton spp. and Rhizopus spp. 1 (1.96%). Besides, samples were examined for the presence of bacteria and one or more of the bacteria were also isolated from 14 (27.4%) of 51 samples. This assessment in the veterinary microbiology laboratory has shown that the isolated fungi agents pose a significant risk of infection for people who take care of these animals or consume animal products and pet owners.

Effect of chlorogenic and gallic acids combined with azoles on antifungal susceptibility and virulence of multidrug-resistant Candida spp. and Malassezia furfur isolates

2020 ◽  
Vol 58 (8) ◽  
pp. 1091-1101
Author(s):  
Wafa Rhimi ◽  
Chioma Inyang Aneke ◽  
Giada Annoscia ◽  
Domenico Otranto ◽  
Teun Boekhout ◽  
...  
Keyword(s):  
Extracellular Enzymes ◽  
Antifungal Susceptibility ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Inhibitory Effect ◽  
High Morbidity ◽  
Candida Spp ◽  
Pichia Kudriavzevii ◽  
Malassezia Spp ◽  
Time Kill

Abstract Chlorogenic acid (CHA) and gallic acid (GA) are safe natural phenolic compounds that are used as enhancers of some drugs in influencing antioxidant, anticancer, and antibacterial activities. Among fungi, Candida spp. and Malassezia spp. are characterized by an increasing prevalence of multidrug resistance phenomena and by a high morbidity and mortality of their infections. No data are available about the efficacy of CHA and GA combined with azoles on the antifungal susceptibility and on the virulence of both fungi. Therefore, their antifungal and antivirulence effects have been tested in combination with fluconazole (FLZ) or ketoconazole (KTZ) on 23 Candida spp. and 8 M. furfur isolates. Broth microdilution chequerboard, time-kill studies, and extracellular enzymes (phospholipase and hemolytic) activities were evaluated, displaying a synergistic antifungal action between CHA or GA and FLZ or KTZ on C. albicans, C. bovina, and C. parapsilosis, and antagonistic antifungal effects on M. furfur and Pichia kudriavzevii (Candida krusei) isolates. The time-kill studies confirmed the chequerboard findings, showing fungicidal inhibitory effect only when the GA was combined with azoles on Candida strains. However, the combination of phenolics with azoles had no effect on the virulence of the tested isolates. Our study indicates that the combination between natural products and conventional drugs could be an efficient strategy for combating azole resistance and for controlling fungistatic effects of azole drugs.

scholarly journals The clinical significance of IgE-antibodies to Malassezia in children with atopic dermatitis

2015 ◽  
Vol 12 (6) ◽  
pp. 34-38
Author(s):  
M A Mokronosova ◽  
E V Turlapova ◽  
A M Glushakova ◽  
A N Pampura ◽  
T M Zheltikova
Keyword(s):  
Atopic Dermatitis ◽  
Clinical Significance ◽  
Skin Diseases ◽  
Control Group ◽  
Lsu Rdna ◽  
Healthy Children ◽  
Ige Antibodies ◽  
Age Related ◽  
Children With Asthma ◽  
Malassezia Spp

Background. The objective was to study the species diversity and the proportion of lipophilic yeasts of the genus Malassezia on the skin of children with atopic dermatitis and control groups, to evaluate age-related features and clinical significance of sensitization to Malassezia and IgE-antibodies to Mn-SOD in children with atopic dermatitis. Materials and methods. The study involved 144 children aged between 1 -14 years: 93 patients with atopic dermatitis, a control group of K1 - 24 children with asthma and /or AR without atopic dermatitis and K2 - 27 healthy children without skin diseases. The yeast Malassezia spp. identified by morphological and physiological characteristics and analysis of the nucleotide sequences of the D1/D2 region of 26S (LSU) rDNA. For detection of IgE antibodies to various allergens, including Malassezia, used the device and reagents ImmunoCap (Thermofisher, Sweden); IgE-antibodies to Mn-SOD was detected by the method of ISAC ImmunoCap (Thermofisher, Sweden). Results. Identified 5 species of Malassezia, absolutely dominated M. sympodialis. IgE-antibodies to Malassezia spp. were detected in 45% (42/93) of patients with atopic dermatitis: 11% - children with ADL, 22% from the ADB and 67% from the ADT. Statistically significant correlation between the level of IgE-antibodies to the yeast Malassezia spp. and severity of atopic dermatitis (SCORAD) - r=0,5928, p

NEW IN CLINICAL LABORATORY DIAGNOSTICS OF DERMATOPHYTE FUNGI IN CHILDREN WITH ATOPIC DERMATITIS

2019 ◽  
Vol 64 (6) ◽  
pp. 351-353
Author(s):  
V. G. Arzumanian ◽  
I. V. Il`ina ◽  
G. V. Vasilieva ◽  
O. O. Magarshak ◽  
A. Yu. Mironov
Keyword(s):  
Atopic Dermatitis ◽  
Clinical Laboratory ◽  
Tween 80 ◽  
Medical Personnel ◽  
Laboratory Diagnostics ◽  
Candida Spp ◽  
Seeding Method ◽  
Malassezia Spp ◽  
Staphylococcus Spp ◽  
First Time

Role of bacteria Staphylococcus spp., yeasts of Candida spp., Malassezia spp. genera in pathogenesis of atopic dermatitis (AD) in infant patients is well known. However, no data concerning the incidence of dermatophytes in such disease entity were obtained. Aim of the study was estimation of dermatophytes carriage in children with AD. Group of patients involved 44 persons 1 to 18 years old with clinically diagnosed acute AD. Especially for the study a method of skin scales collection was created: painless for patients and safe for medical personnel. The method is based on following stages - rubbing of lichenificated skin zones with sterile swab which was preliminary saturated with phosphate/Tween 80 buffer pH 7,9 and centrifuging of the suspension for sedimentation of skin scales. Microscopic examination of hydrolyzed scales was carried out at maximal magnification x1750, at that different dermatophyte and yeast fungal forms were registered. Spores of dermatophyte fungi were detected with 67,0% frequency, whereas dermatophyte mycelium - with 18,3% frequency. No correlation between dermatophyte spores and mycelium was found out (Pirson`s coefficient r=0,236), however no mycelium without spores was detected, but in 73,3% of cases spores occurred without mycelium. Yeast spores were detected in 45,2% of patients - this fact is agreed with data obtained earlier by the seeding method, but yeast mycelium was found out in 3,5% of patients. Therefore frequent occurrence of dermatophyte fungi on the skin of AD infants is revealed for the first time. At that dermatophytes were found out to exist more often in the form of spores. These results are important for choice of the further therapy.

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