scholarly journals ANTIOXIDATIVE DEFENSE RESPONSE TO APHID-INDUCED OXIDATIVE STRESS IN Glycine max (L.) Merr. cv. “Nam Dan”

2016 ◽  
Vol 54 (6) ◽  
pp. 719 ◽  
Author(s):  
Tran Ngoc Toan ◽  
Tran Thi Van Thanh Huyen ◽  
Mai Van Chung
Keyword(s):  
Oxidative Stress ◽  
Glycine Max ◽  
Oxidative Damage ◽  
Thiobarbituric Acid ◽  
Ros Generation ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Aphis Craccivora Koch ◽  
Glycine Max L ◽  
Aphid Feeding

Infestation of cowpea aphid (Aphis craccivora Koch) induced oxidative stress in leaves of soybean (Glycine max (L.) Merr. cv. “Nam Dan”) with a burst in generation of reactive oxygen species (ROS) products such as superoxide anion radical (O2.-) and hydrogen peroxide (H2O2) recorded around 24 hours after aphid feeding. An increase in content of thiobarbituric acid reactive substances (TBARS) in lipid peroxidation and a defined percentage of injury in aphid-infested leaves were resulted from the cellular oxidative damage. The enhanced activity of the antioxidant enzymes such as superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6) in leaves of soybean “Nam Dan” functions as the antioxidative response that controlled both ROS-generation to be enough levels to play as defensive element and ROS-detoxifying to reduce aphid-induced oxidative damage. The enhancement of SOD and CAT also can improve the tolerance of soybean “Nam Dan” to impact from A. craccivora.

scholarly journals Effects of [Cu(2-MeSNic)2(MeNia)2(H2O)2]·H2O and [Cu(2-MeSNic)2(H2O)]2 on Candida albicans

2015 ◽  
Vol 8 (1) ◽  
pp. 39-43
Author(s):  
Barbora Kaliňáková ◽  
Daniela Hudecová ◽  
Peter Segľa ◽  
Martina Palicová ◽  
Jozef Švorec
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Candida Albicans ◽  
Copper Complexes ◽  
Membrane Damage ◽  
Thiobarbituric Acid ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hoechst 33342

Abstract Probable mode of action of new copper complexes of 2-methylthionicotinate (2-MeSNic) of composition [Cu(2-MeSNic)2(H2O)2] and [Cu(2-MeSNic)2(MeNia)2(H2O)2]·H2O (where MeNia is N-methylnicotinamide) is described. Both partial growth inhibition of Candida albicans (IC50 ≥ 1.78 mmol·L−1, MIC ≥ 2.5 mmol·L−1) and leak of proteins into the extracellular space (more than 80 %) were observed in the presence of these copper complexes. The membrane damage was detected by staining with Hoechst 33342, propidium iodide and methylene blue. Ascorbic acid potentiated antifungal activity of copper complexes approximately seven-fold and induced the oxidative stress, respectively. The production of intracellular reactive oxygen species was visualized by dichlorofluorescein. Thiobarbituric acid-reactive substances were formed as a by-product of lipid peroxidation.

scholarly journals Redox parameters in blood of thyroid cancer patients after the radioiodine ablation

2017 ◽  
Vol 32 (4) ◽  
pp. 358-365
Author(s):  
Vera Spasojevic-Tisma ◽  
Milovan Matovic ◽  
Olgica Mihaljevic ◽  
Snezana Zivancevic-Simonovic ◽  
Marija Jeremic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thyroid Cancer ◽  
Cancer Patients ◽  
Differentiated Thyroid Cancer ◽  
Radioactive Iodine ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Radioiodine Ablation

The radioactive iodine (131I) ablation is a well-accepted treatment modality for differentiated thyroid cancer patients. Unfortunately, the radiation induces the oxidative stress and damages cells and tissues, simultaneously activating the mechanisms of antioxidative defense. Since the mechanisms of those processes are not completely known, we wanted to examine the changes in the most important reactive oxygen species and antioxidative components, as well as their correlation and significance for lipid peroxidation. Our results showed that the level of thiobarbituric acid reactive substances was increased during the first 30 days after the radiotherapy. Among antioxidant components, superoxide dismutase was increased in the 3rd and 30th day; catalase in 7th and reduced glutathione in 3rd and 7th day after the radiotherapy. As regards the prooxidants, the reduction of hydrogen peroxide (H2O2) was recorded in 7th and 30th day, and superoxide anion radical (O?-) was unchanged after the exposure to 131I. These results indicate that differentiated thyroid cancer patients are under constant oxidative stress despite the observed increase in antioxidative and reduction in prooxidative parameters. The understanding of these early processes is important since their progress determines the latter effects of 131I therapy.

scholarly journals Abscisic Acid in Coordination with Nitrogen Alleviates Salinity-Inhibited Photosynthetic Potential in Mustard by Improving Proline Accumulation and Antioxidant Activity

Stresses ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 162-180
Author(s):  
Arif Majid ◽  
Bilal A. Rather ◽  
Asim Masood ◽  
Zebus Sehar ◽  
Naser A. Anjum ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Abscisic Acid ◽  
Photosynthetic Activity ◽  
Thiobarbituric Acid ◽  
Salinity Treatment ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Photosynthetic Potential

This investigation was done to assess the role of abscisic acid (ABA; 25 µM) and/or nitrogen (N; 10 mM) in the alleviation of salinity (NaCl; 100 mM)-induced reduction in photosynthetic activity and growth, N and sulfur (S) assimilation of mustard (Brassica juncea L.) cv. RH0-749. Salinity treatment caused oxidative stress and significantly elevated the content of both H2O2 and thiobarbituric acid reactive substances (TBARS), and impaired photosynthetic activity and growth, but increased the content of nitrogenous osmolyte proline and the activity of antioxidant enzymes involved in the metabolism of reactive oxygen species. The application of 25 µM ABA under a controlled condition negatively affected photosynthesis and growth. However, ABA, when combined with N, minimized oxidative stress and mitigated the salinity-inhibited effects by increasing the activity of antioxidant enzymes (superoxide dismutase, SOD; glutathione reductase, GR; ascorbate peroxidase, APX) and proline content. Overall, the supplementation of 10 mM N combined with 25 µM ABA provides an important strategy for enhancing the photosynthetic potential of B. juncea under saline conditions.

scholarly journals Regulation of Reactive Oxygen Species and Antioxidant Defense in Plants under Salinity

2021 ◽  
Vol 22 (17) ◽  
pp. 9326
Author(s):  
Mirza Hasanuzzaman ◽  
Md. Rakib Hossain Raihan ◽  
Abdul Awal Chowdhury Masud ◽  
Khussboo Rahman ◽  
Farzana Nowroz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Salt Stress ◽  
Oxidative Damage ◽  
Antioxidant Defense ◽  
Reactive Oxygen ◽  
Antioxidant Defense System ◽  
Ros Generation ◽  
Oxygen Species ◽  
Defense System

The generation of oxygen radicals and their derivatives, known as reactive oxygen species, (ROS) is a part of the signaling process in higher plants at lower concentrations, but at higher concentrations, those ROS cause oxidative stress. Salinity-induced osmotic stress and ionic stress trigger the overproduction of ROS and, ultimately, result in oxidative damage to cell organelles and membrane components, and at severe levels, they cause cell and plant death. The antioxidant defense system protects the plant from salt-induced oxidative damage by detoxifying the ROS and also by maintaining the balance of ROS generation under salt stress. Different plant hormones and genes are also associated with the signaling and antioxidant defense system to protect plants when they are exposed to salt stress. Salt-induced ROS overgeneration is one of the major reasons for hampering the morpho-physiological and biochemical activities of plants which can be largely restored through enhancing the antioxidant defense system that detoxifies ROS. In this review, we discuss the salt-induced generation of ROS, oxidative stress and antioxidant defense of plants under salinity.

Oxidative stress and changes in adenosine deaminase activity of cattle experimentally infected by Fasciola hepatica

Parasitology ◽  
2017 ◽  
Vol 144 (4) ◽  
pp. 520-526 ◽  
Author(s):  
ALEKSANDRO S. DA SILVA ◽  
MATHEUS D. BALDISSERA ◽  
NATHIELI B. BOTTARI ◽  
MATHEUS E. GABRIEL ◽  
LEANDRO A. RHODEN ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adenosine Deaminase ◽  
Liver Tissue ◽  
Fasciola Hepatica ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Adenosine Deaminase Activity ◽  
Group A ◽  
Group B

SUMMARYThe aim of this study was to evaluate the oxidative stress in serum and liver and adenosine deaminase (ADA) activity of cattle experimentally infected by Fasciola hepatica. The group A consisted of five healthy animals (uninfected), and the group B was composed of five animals orally infected with 200 metacercariae of F. hepatica. On days 20, 40, 60 and 80 post-infection (PI) serum was collected to measure oxidative stress variables. On day 100 PI, animals were humanely euthanized and liver samples were collected. Infected animals showed lower (P < 0·05) seric ADA activities on days 40 and 60 PI but higher (P < 0·05) in the liver tissue compared with uninfected animals. Seric and hepatic reactive oxygen species (ROS) were higher (P < 0·05) in infected compared with uninfected animals. Hepatic thiobarbituric acid reactive substances were higher (P < 0·05) in infected animals. Catalase and glutathione S-transferase activities were lower in liver tissue of infected animals, while glutathione peroxidase was higher compared with uninfected (P < 0·05). In summary, we conclude that oxidative stress occurs in cattle experimentally infected by F. hepatica, mainly due to excessive ROS production in the course of fasciolosis, contributing to hepatic damage, and that increased in hepatic ADA activity may contribute to the inflammatory process.

An integrated view of oxidative stress in aging: basic mechanisms, functional effects, and pathological considerations

2007 ◽  
Vol 292 (1) ◽  
pp. R18-R36 ◽  
Author(s):  
Kevin C. Kregel ◽  
Hannah J. Zhang
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Physiological Function ◽  
Ros Generation ◽  
Oxygen Species ◽  
Stress Theory ◽  
Complex Process ◽  
Potential Impact ◽  
Critical Aspects

Aging is an inherently complex process that is manifested within an organism at genetic, molecular, cellular, organ, and system levels. Although the fundamental mechanisms are still poorly understood, a growing body of evidence points toward reactive oxygen species (ROS) as one of the primary determinants of aging. The “oxidative stress theory” holds that a progressive and irreversible accumulation of oxidative damage caused by ROS impacts on critical aspects of the aging process and contributes to impaired physiological function, increased incidence of disease, and a reduction in life span. While compelling correlative data have been generated to support the oxidative stress theory, a direct cause-and-effect relationship between the accumulation of oxidatively mediated damage and aging has not been strongly established. The goal of this minireview is to broadly describe mechanisms of in vivo ROS generation, examine the potential impact of ROS and oxidative damage on cellular function, and evaluate how these responses change with aging in physiologically relevant situations. In addition, the mounting genetic evidence that links oxidative stress to aging is discussed, as well as the potential challenges and benefits associated with the development of antiaging interventions and therapies.

Association Between Magnesium and Oxidative Stress in Patients with Obesity

2020 ◽  
Vol 16 (5) ◽  
pp. 743-748
Author(s):  
Ana R.S. de Oliveira ◽  
Kyria J.C. Cruz ◽  
Jennifer B.S. Morais ◽  
Juliana S. Severo ◽  
Jéssica B. Beserra ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Thiobarbituric Acid ◽  
Magnesium Concentration ◽  
Control Group ◽  
Compensatory Mechanism ◽  
Thiobarbituric Acid Reactive Substances ◽  
Magnesium Intake ◽  
Significant Difference ◽  
Dietary Magnesium ◽  
And Control

Background: The role of minerals in preventing the generation of oxidative stress in obese individuals has been evaluated. Magnesium is an antioxidant nutrient and a cofactor of enzymes involved in the cell membrane stabilization, attenuating the effects of oxidative stress. Objective: To evaluate the association between magnesium and concentrations of thiobarbituric acid reactive substances (TBARS) in patients with obesity and eutrophic women. Methods: A cross-sectional study was conducted with 73 women, divided into two groups: case group (patients with obesity, n=27) and control group (eutrophic women, n=46). Measurements of body mass index and waist circumference were performed. Dietary magnesium intake was assessed by the three-day food record using the NutWin software. Urinary magnesium concentration was measured by atomic absorption spectrophotometry method. Plasma concentrations of thiobarbituric acid reactive substances (TBARS) were also determined. Results: Mean values of dietary magnesium intake were 161.59 ± 60.04 and 158.73 ± 31.96 for patients with obesity and control group, respectively, with no significant difference between the groups studied (p >0.05). The value of urinary excretion of magnesium was lower than the reference values in both groups, with no significant difference between the groups studied (p >0.05). The plasma concentration of thiobarbituric acid reactive substances was significantly higher in patients with obesity compared to the control group (p <0.001). There was no correlation between levels of magnesium biomarkers and the concentration of TBARS (p >0.05). Conclusion: Patients with obesity showed a reduced dietary magnesium intake which seems to induce hypomagnesuria as a compensatory mechanism. The marker of oxidative stress evaluated in this study was not influenced by magnesium.

scholarly journals Antioxidant Activity of the Isoflavonoids from the Roots of Maackia amurensis

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Nadezda I. Kulesh ◽  
Sergey A. Fedoreyev ◽  
Marina V. Veselova ◽  
Natalia P. Mischenko ◽  
Vladimir A. Denisenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Glutathione Peroxidase ◽  
Column Chromatography ◽  
Thiobarbituric Acid ◽  
Formalin Injection ◽  
Thiobarbituric Acid Reactive Substances ◽  
H Nmr ◽  
Maackia Amurensis

Seven isoflavonoids, including a new glycoside, (6a R,11a R)-medicarpin-3- O-gentiobioside (6), were isolated from the roots of Maackia amurensis using repeated column chromatography on a Toyopearl HW-50F sorbent and identified by HPLC–PDA–MS, 1H NMR, 13C, 1H–1H COSY, HSQC NMR and HMBC NMR analyses as daidzin (1), genistein-7- O-gentiobioside (2), pseudobaptigenin-7- O-gentiobioside (3), formononetin-7- O-gentiobioside (4), (6a R,11a R)-maackiain-3- O-gentiobioside (5), and 5- O-methylgenistein-7- O-gentiobioside (7). In the model of oxidative stress induced by formalin injection, the isolated isoflavone and pterocarpan glucosides 1-7 were shown to reduce the formation of malondialdehyde (MDA) and other thiobarbituric acid reactive substances (TBARS), as well as glutathione peroxidase (GPO) activity in rats.

scholarly journals Oxidative stress in patients on mechanical ventilation

2009 ◽  
Vol 62 (11-12) ◽  
pp. 578-581
Author(s):  
Vesna Marjanovic ◽  
Vidosava Djordjevic ◽  
Goran Marjanovic
Keyword(s):  
Oxidative Stress ◽  
Mechanical Ventilation ◽  
Head Injuries ◽  
Lipid Peroxide ◽  
Thiobarbituric Acid ◽  
Lactic Dehydrogenase ◽  
Lung Damage ◽  
Thiobarbituric Acid Reactive Substances ◽  
Acid Base Balance ◽  
Arterial Blood

Introduction. The appearance and intensity of oxidative stress were analyzed in the course of mechanical ventilation and parameters that could point toward potential lung damage. Material and methods. In three time intervals on day 1, 3 and 7 of mechanical ventilation, parameters such as: triglycerides, cholesterol, lactate, serum lactic dehydrogenase, acid-base balance and lipid peroxidation products - thiobarbituric acid reactive substances, were followed in 30 patients with head injuries. Results. A decrease in the level of partial oxygen pressure (PaO2) (p<0.01) and PaO2/FiO2 index (p<0.05) in arterial blood was recorded on day 3 of mechanical ventilation. This was accompanied with an increase in alveolar-arterial difference (AaDO2) (p<0.05), thiobarbituric acid reactive substances (p<0.001) and lactic dehydrogenase (p<0.001) comparing to day 1 of mechanical ventilation. The patients with initial PaO2>120 mmHg, had significant increase of thiobarbituric acid reactive substances and AaDO2 (p<0.05) and fall of PaO2 (p<0.001) on day 3 of mechanical ventilation. Conclusion. Oxidative stress and lipid peroxide production are increased during third day of mechanical ventilation leading to disruption of oxygen diffusion through alveolar-capillary membrane and reduction of parameters of oxygenation.

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