Optimization of culture conditions of Streptomyces antibioticus strain 1083 to improve the antimicrobial activity against Aeromonas hydrophila

Fish is a healthy, high protein and low fat food that encourages the health and growth of people, especially children. However, in fact fish is very sensitive to many diseases which affects the productivity and quality of fish. Therefore, identifying the cause of the diseases and finding preventive measures become an urgent task today. In the previous study, we isolated Streptomyces antibioticus strain 1083 that has the ability to antagonize Aeromonas hydrophila - a pathogenic bacterium in fish. Based on the obtained results, we continue to perform this study to determine optimal conditions for the culture of S. antibioticus strain 1083 in order to produce antimicrobial compounds against A. hydrophila. The production of antagonists by the strain 1083 was optimized by controlling the condition of different inoculations such as media, pH, temperature and incubation period. The results indicated that International Streptomyces Project 2 (ISP2) was the best medium for S. antibioticus strain 1083 to produce the highest antimicrobial activity against A. hydrophila with 32 mm in diameter of inhibited zone. The actinomycete strain 1083 could express the maximum antimicrobial activity when they were incubated in shaker incubator (200rpm) at 40oC with pH8 in 8 days. The ability of the actinomycete strain in antagonism against A. hydrophila was evaluated by adding different culture medium volume of S. antibioticus strain 1083. With adding 10% cultured solution volume of S. antibioticus strain 1083 into the culture medium of A. hydrophila, after 1 day of inoculation the number of pathogenic bacteria cells were completely eliminated.

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Efek Suhu dan Masa Simpan Terhadap Aktivitas Antimikroba Susu Fermentasi dengan Lactobacillus casei

Jurnal Agripet ◽

10.17969/agripet.v16i1.3639 ◽

2016 ◽

Vol 16 (1) ◽

pp. 49

Author(s):

Cut Afria Mirdalisa ◽

Yusdar Zakaria ◽

Nurliana Nurliana

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Lactobacillus Casei ◽

Pathogenic Bacteria ◽

Storage Time ◽

Fermented Milk ◽

Effect Of Temperature ◽

Duration Of Storage ◽

And Storage

ABSTRAK. Susu fermentasi merupakan susu hasil pengasaman melalui aktivitas bakteri asam laktat yang menyebabkan perubahan kimia, sensorik dan mikrobiologi dalam produk susu fermentasi. Lactobacillus casei merupakan bakteri yang mampu menghasilkan senyawa organik dan hydrogen peroksida yang bersifat antibakteri. Senyawa antibakteri ini adalah senyawa kimiawi atau biologis yang dapat menghambat pertumbuhan dan aktivitas bakteri patogen yang dapat merusak kualitas susu fermentasi. Penelitian ini bertujuan untuk mengetahui pengaruh suhu dan lama penyimpanan terhadap kualitas susu fermentasi dengan Lactobacillus casei. Suhu dan lama penyimpanan pada susu fermentasi Lactobacillus casei pengaruh sangat nyata (P<0,01) dan dapat menekan pertumbuhan terhadap bakteri E.coli dan Staphylococcus aureus. Aktivitas antimikroba susu fermentasi Lactobacillus casei pada suhu penyimpanan 5⁰C dengan lama penyimpanan 30 hari dapat menekan pertumbuhan bakteri Staphylococcus aureus. (Effects of temperature and storage time on the antimicrobial activity fermented milk with Lactobacillus casei) ABSTRACT. Fermented milk is milk acidification results through the activity of lactic acid bacteria that causes chemical changes, sensory and microbiological in fermented milk products. Lactobacillus casei is a bacteria that can produce organic compounds and hydrogen peroxide which is antibacterial. The antibacterial compound is a chemical or biological compound that can inhibit the growth and activity of pathogenic bacteria that can damage the quality of fermented milk. The study has been conducted to determine the effect of temperature and storage time on the quality of fermented milk with Lactobacillus casei. the temperature and duration of storage time of fermented milk with Lactobacillus casei highly significant (P <0.01) affected and can suppress the growth of bacteria E.coli and Staphylococcus aureus. The antimicrobial activity of Lactobacillus casei fermented milk at the storage temperature of 5⁰C with a storage time of 30 days can suppress the growth of Staphylococcus aureus.

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Uji efektivitas ekstrak daun Andong (Cordyline fruticosa) sebagai bahan antimikroba alami

e-Journal BUDIDAYA PERAIRAN ◽

10.35800/bdp.9.1.2021.31829 ◽

2020 ◽

Vol 9 (1) ◽

Author(s):

Henky Manoppo

Keyword(s):

Antimicrobial Activity ◽

Aeromonas Hydrophila ◽

Pathogenic Bacteria ◽

Leaf Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Natural Antimicrobial ◽

Cordyline Fruticosa ◽

Growth Of Bacteria

The purposed of research was to evaluate the ability of Andong leaf extract as natural antimicrobial against pathogenic bacteria, Aeromonas hydrophila. The plant was collected from yards at Kasuang Village, Tondano Disctrict of Minahasa Regency. After washing, the leaf was cut into small pieces, eased using a mortar and then extracted by maceration using alcohol 75% for 24 hours with ratio between simplicia and solvent was 1:1, 1:2, 1:4 and 1:8. Antimicrobial activity of the extract was examined through disc diffusion method. The results showed that leaf extract of andong was able to inhibit the growth of bacteria with strong and the sensitivity of the extract was categorized as strong.

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EVALUASI PEMBERIAN PROBIOTIK BACILLUS PADA MEDIA PEMELIHARAAN TERHADAP LAJU PERTUMBUHAN DAN PERUBAHAN HISTOPATOLOGI IKAN LELE DUMBO (Clarias gariepinus) YANG DIINFEKSI Aeromonas hydrophila

Jurnal Riset Akuakultur ◽

10.15578/jra.11.2.2016.171-179 ◽

2016 ◽

Vol 11 (2) ◽

pp. 171 ◽

Cited By ~ 1

Author(s):

Angela Mariana Lusiastuti ◽

Mohammad Faizal Ulkhaq ◽

Widanarni Widanarni ◽

Tri Heru Prihadi

Keyword(s):

Growth Rate ◽

Aeromonas Hydrophila ◽

Pathogenic Bacteria ◽

Culture Medium ◽

Clarias Gariepinus ◽

Histopathological Change ◽

Positive Control ◽

Negative Control ◽

African Catfish ◽

Liver And Kidney

The aim of this study was to evaluate the addition of a probiotic Bacillus on culture medium through growth rate and histopathological change in African Catfish who was infected by Aeromonas hydrophila. The study consisted of five treatments such as the addition of probiotic Bacillus ND2, Bacillus P4I2, Bacillus ND2 + Bacillus P4I2 (Kom), positive control (K+) and negative control (K-) (without probiotic addition). African Catfish (13.354±2.8 g) was maintained in 15 aquariums (40 L in volume) with 30 fishes each for 30 days. Probiotic bacteria was applied in water once a day, whereas pathogenic bacteria A. hydrophila (103 CFU/mL) were added once in earlier treatment (except for the negative control) as assumption that A. hydrophila will shape up quorum sensing in unfortunate condition. The result showed that the highest spesific growth rate in the treatment of Bacillus ND2 probiotics (1,708 ± 0.114%). Histopathology showed that damaged of liver dan kidney in probiotics treatment were lightner than positive control (K+). The addition of Bacillus P4I1 (104 CFU/mL) efective to increased the spesific growth rate of African Catfish and prevent the damage of liver and kidney of African Catfish was infected by Aeromoniasis.

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Bacteriophages with Potential to Inactivate Aeromonas hydrophila in Cockles: In Vitro and In Vivo Preliminary Studies

Antibiotics ◽

10.3390/antibiotics10060710 ◽

2021 ◽

Vol 10 (6) ◽

pp. 710

Author(s):

João Duarte ◽

Carla Pereira ◽

Pedro Costa ◽

Adelaide Almeida

Keyword(s):

Aeromonas Hydrophila ◽

Pathogenic Bacteria ◽

Culture Medium ◽

Phage Therapy ◽

Maximum Reduction ◽

Liquid Culture Medium ◽

Resistant Mutants ◽

Bivalve Shellfish

The recurrent emergence of infection outbreaks associated with shellfish consumption is of extreme importance for public health. The present study investigated the potential application of phages AH-1, AH-4, and AH-5 to inactivate Aeromonas hydrophila, a causative agent of infections in humans associated with bivalve shellfish consumption. The inactivation of A. hydrophila was assessed in vitro, using a liquid culture medium, and in vivo, using artificially contaminated cockles with A. hydrophila ATCC 7966. In the in vitro experiments, all phages were effective against A. hydrophila, but phage AH-1 (with a maximum reduction of 7.7 log colonies forming units CFU/mL) was more effective than phages AH-4 and AH-5 (with reductions of 4.9 and 4.5 log CFU/mL, respectively). The cocktails AH-1/AH-4, AH-1/AH-5, AH-4/AH-5, and AH-1/AH-4/AH-5 were slightly more effective than the single phage suspensions. The phages presented a low emergence rate of phage-resistant mutants. When artificially contaminated cockles were treated in static seawater with phage AH-1, around 44% of the added A. hydrophila (1.0 log CFU/g) was inactivated. The results of this study suggest that phage therapy can be an effective alternative to control human pathogenic bacteria during depuration.

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EFFICIENT MANAGEMENT SYSTEM OF TEACHING TECHNICAL MEANS AT CENTRAL KINDERGARTEN PEDAGOGY COLLEGE IN INDUSTRIAL REVOLUTION 4.0

Tạp chí Nghiên cứu dân tộc ◽

10.25073/0866-773x/425 ◽

2020 ◽

Vol 9 (2) ◽

Author(s):

Vũ Xuân Hùng

Keyword(s):

Intellectual Development ◽

Industrial Revolution ◽

Current Period ◽

Efficient Management ◽

Urgent Task ◽

Creative Learning ◽

Cognitive Activities ◽

And Control ◽

Technical Teaching

In the process of teaching, technical teaching facilities are both a content and a means of conveying information, they help the lecturer organize and control the students' cognitive activities, in addition, they also help students be interested in learning, practice practical skills from which to form active and creative learning methods. Teaching technology is one of the necessary conditions to help teachers carry out their related work of educating, teaching and bringing up, and intellectual development, arouse the inherent intelligence qualities of students. Currently, the management of technical teaching facilities at the Central Kindergartens College has been carried out on a regular basis and achieved certain results, but in fact, there are still many inadequacies. Finding a number of limitations in the management of teaching technical facilities, thereby proposing solutions to overcome those limitations, improve the efficiency of investment, preservation and use of teaching technical facilities in the trend of Industry Revolution 4.0, improving the quality of teaching at Central Kindergarten Pedagogy colleges in the current period is a very important and urgent task.

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ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.28930/jitkt.v1i2.7871 ◽

2009 ◽

Vol 1 (2) ◽

Author(s):

Risa Nofiani ◽

Siti Nurbetty ◽

Ajuk Sapar

Keyword(s):

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Pathogenic Bacteria ◽

Methanol Extract ◽

Agar Medium ◽

Antimicrobial Activities ◽

Antimicrobial Compounds ◽

Associated Bacteria ◽

Minimum Inhibitory Concentrations ◽

Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

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Effects of Extract of Lactic Acid Bacteria Culture Media on Quality Characteristics of Pork Loin and Antimicrobial Activity against Pathogenic Bacteria during Cold Storage

Journal of the Korean Society of Food Science and Nutrition ◽

10.3746/jkfn.2016.45.10.1476 ◽

2016 ◽

Vol 45 (10) ◽

pp. 1476-1480

Author(s):

Yun Ji Lee ◽

Kwang Suk Ko

Keyword(s):

Antimicrobial Activity ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Cold Storage ◽

Pathogenic Bacteria ◽

Culture Media ◽

Quality Characteristics ◽

Pork Loin ◽

Bacteria Culture

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Microbiological quality of residues from drinking water preparation

Water Science & Technology ◽

10.2166/wst.1995.0565 ◽

1995 ◽

Vol 31 (5-6) ◽

pp. 75-79 ◽

Cited By ~ 5

Author(s):

M. Würzer ◽

A. Wiedenmann ◽

K. Botzenhart

Keyword(s):

Waste Water ◽

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

Drinking Water ◽

Water Treatment ◽

Pathogenic Bacteria ◽

Waste Water Treatment ◽

Microbiological Quality ◽

Detection Limits

In Germany the application of procedures such as flocculation and filtration in the preparation of drinking water results in the annual production of an estimated 500,000 t of sediments and sludges. Some of these residues have a potential for being reused, for example in agriculture, forestry, brickworks or waste water treatment. To assess the microbiological quality of residues from waterworks methods for the detection of enterobacteria, Escherichia coli, Salmonella, Pseudomonas aeruginosa, Legionella, poliovirus, Ascaris suis eggs and Cryptosporidium have been evaluated regarding their detection limits and were applied to various residues from German waterworks. Results show that sediments and sludges may contain pathogenic bacteria, viruses and protista. When residues from waterworks are intended to be reused in agriculture or forestry the microbiological quality should therefore be considered.

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