scholarly journals State of the immune system in women using the “Provag” probiotic

2020 ◽  
Vol 22 (4) ◽  
pp. 785-790
Author(s):  
V. A. Kozlov ◽  
A. G. Borisov ◽  
A. A. Savchenko ◽  
A. E. Kondakov ◽  
I. V. Kudryavtsev
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Lactobacillus Plantarum ◽  
B Lymphocytes ◽  
Humoral Immunity ◽  
Lactobacillus Fermentum ◽  
Passive Hemagglutination ◽  
Specific Antibodies ◽  
Lactobacillus Gasseri ◽  
Healthy Women

Lactobacilli are  widely used  in clinical  practice as probiotics, biologically  active  additives  and probiotic products for functional nutrition. Some  probiotics can  be considered as bacterial vaccines  due  to induction of immune response, accompanied by production of specific antibodies. The aim of the present study was to evaluate  the state of cellular and humoral immunity in women  by using probiotic strains of lactobacilli. The  study  included 31 healthy  women  aged  25-45  years.  As a source  of probiotic lactobacterial complex, we used  the  “Provag” preparation (RU  77.99.11.003.E.003746.02.11 of 11.02.2011, 1 capsule  contains 109 Lactobacillus  gasseri 57C,  Lactobacillus  fermentum  57A и Lactobacillus  plantarum  57B).  The  drug  was used for 30 days, at a rate  of one  capsule  per day. The  immune system was examined twice: before  administering the drug and after 30 days of treatment. The study of blood  lymphocyte populations and subpopulations was performed by flow cytometry using direct immunofluorescence technique. The concentration of IgA, IgM, IgG in blood serum was determined using enzyme  immunoassay. To determine specific antibodies, we used passive hemagglutination reaction with erythrocyte diagnosticum. The complex of probiotic lactobacilli Lactobacillus gasseri, Lactobacillus  fermentum  and  Lactobacillus  plantarum corresponding to the “Provag” preparation was used as a source  of antigen. It has been revealed  that  the number of T and B lymphocytes in peripheral blood increased after  30 days  of treatment with  the  probiotic preparation “Provag” in  healthy  women. Elevated contents of T cells was due to the T helper  cell fraction. Increased levels of T helpers and B lymphocytes were associated with stimulation of humoral immunity, as evidenced by increasing concentration of IgA and  IgG in blood  serum.  By means  of passive hemagglutination reaction, we have found  that  90% of healthy  women showed increased concentrations of specific IgA in blood after 30 days of treatment with “Provag” preparation.

scholarly journals IMMUNOREGULATION OF BLOOD SERUM ESTRADIOL AND PROGESTERONE LEVELS IN POSTMENOPAUSAL WOMEN

2019 ◽  
Vol 21 (5) ◽  
pp. 869-876
Author(s):  
A. N. Glushkov ◽  
E. G. Polenok ◽  
S. A. Mun ◽  
L. A. Gordeeva ◽  
V. A. Lutsenko ◽  
...  
Keyword(s):  
Blood Serum ◽  
Human Blood ◽  
Solid Phase ◽  
Biological Effects ◽  
Serum Levels ◽  
Human Blood Serum ◽  
Breast Cancer Patients ◽  
Specific Antibodies ◽  
Healthy Women ◽  
Antibody Levels

Specific antibodies against estradiol (Es) and progesterone (Pg) are known to modulate blood serum concentrations of these hormones and their biological effects after immunization of animals. It was suggested that specific IgA-Es and IgA-Pg could influence on Es and Pg levels in human blood serum. The purpose of this study was to identify the suggested correlations between serum Es and Pg and specific IgA-Es and IgA-Pg in postmenopausal healthy women (HW) and breast cancer patients (BCP). The serum levels of Es, Pg, IgA-Es and IgA-Pg were studied in 226 HW and 633 BCP by means of solid-phase immunoassay. The following results were obtained. The levels of Es in BCP (0.25 nmol/l) were higher than in HW (0.16; р < 0.0001). The levels of Pg were lower (0.79 vs 0.87; р < 0.0001), and individual Pg/Es ratios were lower (3.19 vs 6.64; р < 0.0001). Individual IgA-Pg/IgA-Es ratios correlated with decrease of Es (rs = -0.15; p = 0.029), with increase in Pg (rs = 0.38; р < 0.0001), and with increased Pg/Es ratio (rs = 0.29; р < 0.0001) in healthy women. Similar correlations were determined in BCP (correspondingly: rs = -0.14, р < 0.001; rs = 0.1, р = 0.009; rs = 0.15, р < 0.0001). The decrease of Es and increase of Pg and Pg/Es in BCP were less significant than in HW: the a quotients in regression у = ах+b (y = hormones levels and x = antibodies levels) in BCP were 3 to 4-fold lower than in HW. These peciliarities of interrelations between hormones and specific antibody levels were revealed only in ER+/PR+ BCP but not in ER+/PR- and ER-/PR- BCP. In conclusion, we have confirmed a suggestion about participation of specific antibodies in regulation of steroids levels in human blood serum. The immune regulation of hormonal status was weakened in BCP.

INDICES OF IMMUNE RESPONSES OF BULLS IN THE NECROBACILLOSIS VACCINATION OF THE CATTLE

2015 ◽  
Vol 10 (3) ◽  
pp. 93-97
Author(s):  
Михеева ◽  
Ekaterina Mikheeva ◽  
Бабинцева ◽  
Tatyana Babintseva ◽  
Макаев ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Immune Responses ◽  
Blood Serum ◽  
B Lymphocytes ◽  
Metabolic Disorders ◽  
Specific Antibodies ◽  
Biological Safety ◽  
Digestive Organs ◽  
Udmurt Republic ◽  
And Control

Metabolic disorders and dysfunctional state of the digestive organs lead to the development of distal extremities diseases. The greatest danger is neсrobacrillosis. Vaccination is one of the ways to prevent and control this disease. The article reflects the data on the effect of vaccination (“Nekovak” and formol-emulsion neсrobacrillosis vaccine of Federal Centre for toxicology, radiation and biological safety) on immunological and serological parameters of blood and blood serum of cattle in the Udmurt Republic. After immunization with “Nekovak” vaccine and formol-emulsion neсrobacrillosis vaccine, we determined an increase of the number of leukocytes to 7th day, and also an increase of T-lymphocytes to 14th day. The number of B-lymphocytes reaches a maximum on 21st day. The content of gamma globulins, including specific antibodies against the neсrobacrillosis pathogen, exceeded the control and reached a maximum on the 21st day after vaccination.

scholarly journals СОСТОЯНИЕ ГУМОРАЛЬНОГО ИММУНИТЕТА, КАК ВАЖНЫЙ ПРОГНОСТИЧЕСКИЙ КРИТЕРИЙ ЭФФЕКТИВНОСТИ ПРОГРАММ ЭКСТРАКОРПОРАЛЬНОГО ОПЛОДОТВОРЕНИЯ ПРИ ТРУБНО-ПЕРИТОНЕАЛЬНОМ БЕСПЛОДИИ

2021 ◽  
Vol 7 (4(58)) ◽  
pp. 16-18
Author(s):  
ЮЛИЯ СЕРГЕЕВНА ЧЕХОВА ◽  
СВЕТЛАНА ВЛАДИМИРОВНА СОЛОВЬЁВА
Keyword(s):  
Immune System ◽  
Humoral Immunity ◽  
Immune Status ◽  
Reproductive Technologies ◽  
Main Group ◽  
Pregnancy Planning ◽  
Healthy Women ◽  
Preparation Stage

The indicators of humoral immunity in clinically healthy women at the stage of pregnancy planning and in patients with pipe-peritoneal infertility in the pre-infantal period of vitro incense fertilization periods are studied. The initial immune status in women of the main group did not have pathological deviations. The identified shifts in the work of the immune system in women with infertility, depending on the outcomes of the auxiliary reproductive technologies, was carried out in a multidirectional nature and could serve as a factor in a preventive forecast, in order to ensure a more differentiated approach at the preparation stage and during extracorporeal fertilization.

Peculiarities of immunophysiological shifts in adaptation to climatic and geographical conditions of mountains

2021 ◽  
Author(s):  
N.B. Tyumonbaeva ◽  
A.A. Kazybekova ◽  
N.J. Mamytova ◽  
A.J. Myrzakulova
Keyword(s):  
Immune System ◽  
B Lymphocytes ◽  
Humoral Immunity ◽  
Immune Complexes ◽  
Mixed Type ◽  
Prolonged Exposure ◽  
Healthy Population ◽  
Natural Factors ◽  
Key Words Adaptation ◽  
Cellular And Humoral Immunity

The functioning of the immune system of people exposed to prolonged exposure to natural factors has been monitored and the main immunity indicators have been studied in practically healthy population of different mountain heights in Chui and Naryn regions. Indicators of specific immunity in the residents of the zone of compensated discomfort is reduced compared with the standards of the zone of relative comfort and refers to the mixed type with suppression of cellular and humoral immunity, apparently, this is associated with the climatic and geographical and environmental characteristics of the region. Key words: adaptation, immune system, T- lymphocytes, B- lymphocytes, immunoglobulins, circulating immune complexes, mountainous conditions.

scholarly journals The Role of Plant-Based Protein Functional Food in Preventing Acute Respiratory Disease: A Case Study

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 2116
Author(s):  
Andrei V. Tarasov ◽  
Rofail S. Rakhmanov ◽  
Elena S. Bogomolova ◽  
Ludmila A. Perminova ◽  
Zhanna L. Malakhova
Keyword(s):  
Immune Response ◽  
Treatment Group ◽  
Blood Cell ◽  
University Students ◽  
Blood Serum ◽  
Humoral Immunity ◽  
Functional Food ◽  
Respiratory Diseases ◽  
Protein Product ◽  
Control Group

The Kaliningrad region is known for its specific climate, which can negatively affect the adaptive potential of the body. This manifests in an increased incidence of respiratory diseases and skin conditions. To prevent high morbidity, a plant protein product was included in the diet of first-year university students. This study aimed to assess the effectiveness of this food intervention in preventing the most common diseases among Kaliningrad students. Two groups of university students took part in the food trial. In the control group, catabolic processes prevailed in nutrient metabolism. Disadaptation manifested itself in the metabolism of proteins, vitamins, minerals, hematopoiesis and humoral immunity. Inflammation was indicated by α1- and α2-globulins, a weak immune response, and IgM and IgG. High oxidative stress and low antioxidative ability of blood serum were observed. The plant-based protein product (FP) helped preserve testosterone level and prevent an increase in catabolic reactions. Moreover, it had a positive effect on both red blood cell hematopoiesis (a smaller increase in the average volume of erythrocytes, the same average concentration and content of hemoglobin, an increased relative red cell distribution width (RDW) and white blood cell hematopoiesis (a beneficial effect for the immune system: lymphocytes, the relative content of neutrophils, monocytes, basophils and eosinophils). The stimulation of humoral immunity was evidenced by beta- and gamma-globulins, an active immune response, the level of IgM and IgG, antioxidant protection, reduction of peroxides and an increase in antioxidant activity of blood serum. The 34-week observation showed a 1.7-fold decrease in the incidence of respiratory illnesses and a 5.7-fold decrease in skin and subcutaneous tissue diseases. Acute respiratory infections were reduced 1.8-fold. There were no cases of community-acquired pneumonia in the treatment group, compared with 55.1‰ in the control group. The incidence of respiratory diseases was 3.3–10.6 times lower in the treatment group than in the control group in weeks 6–19. The findings testify to the prophylactic effect of functional food during social adaptation and acclimatization of students.

scholarly journals A REPORT ON ANTIMENINGITIS VACCINATION AND OBSERVATIONS ON AGGLUTININS IN THE BLOOD OF CHRONIC MENINGOCOCCUS CARRIERS

1918 ◽  
Vol 28 (4) ◽  
pp. 449-474 ◽  
Author(s):  
Frederick L. Gates
Keyword(s):  
Blood Serum ◽  
Salt Solution ◽  
Blood Stream ◽  
Severe Reaction ◽  
Meningeal Irritation ◽  
Specific Antibodies ◽  
Individual Susceptibility ◽  
Normal Controls

1. A meningococcus vaccine suspended in salt solution has been given subcutaneously as a prophylactic to about 3,700 volunteers in three injections of 2,000 million, 4,000 million, and 4,000 or 8,000 million cocci at weekly intervals. 2. These doses rarely caused more than the mildest local and general reactions. Exceptionally a more severe reaction emphasized the presence of an unusual individual susceptibility to the vaccine. In such instances the symptoms were in part those of meningeal irritation and sometimes simulated the onset of meningitis. 3. Specific meningococcus agglutinins have been demonstrated in the blood serum of vaccinated men as compared with normal controls. 4. Moreover, agglutinins have been demonstrated in the blood serum of chronic carriers of the meningococcus. Evidence is thus brought forward that the relative immunity of chronic carriers to epidemic meningitis may be due to the presence of specific antibodies in the blood stream.

A Single-Step Gold Nanoparticle–Blood Serum Interaction Assay Reveals Humoral Immunity Development and Immune Status of Animals from Neonates to Adults

2018 ◽  
Vol 5 (2) ◽  
pp. 228-238 ◽  
Author(s):  
Tianyu Zheng ◽  
John Crews ◽  
Jodi L. McGill ◽  
Kunal Dhume ◽  
Caroline Finn ◽  
...  
Keyword(s):  
Blood Serum ◽  
Gold Nanoparticle ◽  
Humoral Immunity ◽  
Immune Status ◽  
Single Step

scholarly journals The cell protection of weated pigs for probiotics

2020 ◽  
pp. 111-119
Author(s):  
L. Bondarenko
Keyword(s):  
Immune System ◽  
Blood Serum ◽  
Phagocytic Activity ◽  
Lysozyme Activity ◽  
The State ◽  
Natural Resistance ◽  
Phagocytic Index ◽  
Control Group ◽  
Stressful Situation ◽  
Experimental Group

The immune system is central to ensuring the consistency of the body's homeostasis. The state of the body's natural resistance is determined by a set of non-specific protective mechanisms. Lymphocytes and phagocytes are actively involved in maintaining immunity. Lymphocytes recognize the antigens of pathogenic microorganisms, and phagocytes absorb and destroy the pathogens themselves. During the weaning of piglets from sows there is a decrease in the protective forces of their body. During this period, the natural resistance of the piglets is reduced due to the stressful situation caused by changing conditions of confinement, the transition to full feed and lack of sows. The immune system of weaning pigs is relatively weak, so when exposed to environmental and technological stressors, they become susceptible to various diseases. The use of probiotic drugs stimulates the activity of the immune system, prevents stress and immunodeficiency. One of these probiotics is the probiotic of domestic production Protecto-active. It w observed the the influence of the probiotic Protecto-active on the indices of nonspecific resistance of the young pigs organism to the growth. An increase in bactericidal activity of blood serum by 12.10% (P <0.05) and lysozyme activity of blood in the piglets of the experimental group was increased by 3.71% compared to control, which indicates the activation of the body's defenses and the increase in adaptive capacity. An important step in the study of the influence of the probiotic Protekto-active on the state of the immune system is to determine the phagocytic activity of neutrophils, phagocytic index and phagocytic number. In the experimental group of piglets that were fed the probiotic Protecto-active, we found an increase in leukocyte phagocytic activity by 9.0% (P <0.001), a phagocytic index by 51.7% (P<0.001) and a phagocytic number by 24.8% ( P <0.01) compared with the control group. Thus, using a probiotic Protecto-active, all indicators of phagocytosis increase: the number of phagocytes increases, their ability to capture microorganisms and increases their digestive capacity, it increases the bacterial and lysozyme activity of blood serum, which is positively reflected in the immunobiosity. Key words: probiotic, phagocytosis, phagocytic index, phagocytic number, phagocytic activity of leukocytes, cellular immunity, piglets

Sign in / Sign up

Export Citation Format

Share Document