Indirect effect of neem oil on Podisus nigrispinus (Hemiptera, Pentatomidae): biology and predatory capacity

This study evaluated the effects on the development and predatory capacity of Podisus nigrispinus fed on Spodoptera frugiperda that have ingested different concentrations of neem oil. The predatory capacity of Podisus nigrispinus was assessed, separating nymphs (fourth instar) and adults (males and females). The treatments consisted of S. frugiperda larvae reared in neem oil aqueous solutions (0.077, 0.359 and 0.599%), deltamethrin EC 25 (0.100%) and control arranged in a completely randomized design, with ten replicates. Insects were offered three larval densities (one, three and six), in the third or fourth instars. The predated larvae were examined at 24 and 48 hours after the beginning of the experiment. Biological parameters of Podisus nigrispinus were evaluated in groups of ten second-instar nymphs transferred to pots, in five replicates. Insects were offered 2-6 third and/or fourth-instar larvae reared in the same neem oil concentrations in a completely randomized design. The following parameters were evaluated: duration of each nymph stage (days), nymph mortality (%), weight of fifth-instar nymphs (mg), sex ratio, weight of males and females (mg) and longevity of unfed adults (days). The predatory capacity of nymphs and adults of Podisus nigrispinus was influenced by the neem oil at the concentrations of 0.359% and 0.599% in the highest density. The concentration of 0.359% lengthened the nymphal stage and the concentration of 0.599% reduced the weight of males.

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Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v7i2.10886 ◽

2020 ◽

Vol 7 (2) ◽

pp. 119

Author(s):

Lukman Affandhy ◽

Muchamad Luthfi ◽

Dian Ratnawati ◽

Frediansyah Firdaus

Keyword(s):

Data Analysis ◽

Analysis Of Variance ◽

Research Method ◽

Moringa Oleifera ◽

The Third ◽

Randomized Design ◽

Leaf Powder ◽

Completely Randomized Design ◽

Second Period

ABSTRAK Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci: kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

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Effect of Ethephon and Packaging Practices On Ripening of Mango cv. Maldah

SAARC Journal of Agriculture ◽

10.3329/sja.v19i1.54786 ◽

2021 ◽

Vol 19 (1) ◽

pp. 155-163

Author(s):

S Sapkota ◽

D Kc ◽

H Giri ◽

M Saud ◽

M Basnet ◽

...

Keyword(s):

Chemical Properties ◽

Control Treatment ◽

Quality Performance ◽

Plastic Packaging ◽

Randomized Design ◽

Acid Ratio ◽

Ethephon Treatment ◽

Completely Randomized Design ◽

And Control ◽

Juice Content

The present research was conducted on two factor Completely Randomized Design (CRD) with eight treatments and three replications. A set of experiments were carried out to evaluate the effect of postharvest ethephon treatment and packaging on ripening of mango cv. Maldah. The treatments consisted of ripening agent i.e., ethephon and control treatment under different packaging condition i.e., fiber with hole, fiber without hole, plastic with hole and plastic without hole. The result revealed that different packaging condition and ripening agents influenced the ripening behavior of mango. The highest TSS (15.26), sugar-acid ratio (23.66) and juice content (126.05) were recorded with fiber (without hole) and the lowest TSS (12.60), sugar-acid ratio (9.01) and juice content (116.05) with plastic (without hole). The highest TA (1.44) was recorded with plastic (without hole) and the lowest (0.66) with fiber (without hole). Similarly, the highest BT (2.83) was recorded with fiber (with hole) and the lowest (1.66) with plastic (without hole). Firmness, sweetness, TSS and juice content were the highest with the interaction effect of fiber bag (without hole) and ethephon treatment. In conclusion, mango fruits with ethephon treatment packed in fiber bag (without hole) enhances quality and ripening of mango whereas under controlled condition and without hole plastic packaging mangoes had low quality performance in terms of physio-chemical properties. SAARC J. Agric., 19(1): 155-163 (2021)

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Effect of pasteurization and starter cultures on physicochemical and microbiological properties of costeño cheese

Revista Facultad Nacional de Agronomía Medellín ◽

10.15446/rfna.v69n2.59145 ◽

2016 ◽

Vol 69 (2) ◽

pp. 8007-8014 ◽

Cited By ~ 3

Author(s):

José G. Serpa ◽

Tulia I. Pérez ◽

Elvis J. Hernández

Keyword(s):

Lactococcus Lactis ◽

Control Sample ◽

Raw Milk ◽

Starter Cultures ◽

Sensorial Analysis ◽

Pasteurized Milk ◽

Randomized Design ◽

Completely Randomized Design ◽

And Control ◽

Microbiological Properties

The effect of pasteurization and starter cultures on physicochemical, microbiological and sensorial characteristics of costeño cheese was determined. A completely randomized design was conducted, three treatments (T) and three replicates: Treatment 1 (T1): cheese manufactured with pasteurized milk without starter cultures, Treatment 2 (T2): cheese manufactured with pasteurized milk with Lactococcus lactis and Lactococcus cremoris (1:1) and Treatment 3 (T3): cheese manufactured with pasteurized milk with Lactococcus lactis, Lactococcus cremoris and Streptococcus thermophillus(0.5:0.5:1). Treatments were compared to a control sample that was prepared with raw milk without starter cultures. Concentration of 1.5% (v/v) of culture was used in relation to the amount of used milk in each treatment. Moisture content was higher in all treatments compared to the control and protein and fat content were significantly lower. Acidity was significantly higher in samples from T2 y T3 compared to T1 and control, due to the metabolism of starter cultures. Total coliforms, yeast and mold counts showed a significant reduction due to pasteurization process in all treatments. Regarding sensorial analysis, hedonic test showed a greater preference in cheese manufactured with T2 (P<0.05). There were no significant preferences between T1, T3 and control. Additionally, yield was significantly higher with T1 (22%) and T3 (23%) compared to control.

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Effect of nutrition on the body temperature and relative organ weights of broilers

Semina Ciências Agrárias ◽

10.5433/1679-0359.2015v36n6supl2p4575 ◽

2015 ◽

Vol 36 (6Supl2) ◽

pp. 4575

Author(s):

Julyana Machado da Silva Martins ◽

Evandro De Abreu Fernandes ◽

João Paulo Rodrigues Bueno ◽

Carolina Magalhães Caires Carvalho ◽

Fernanda Heloisa Litz ◽

...

Keyword(s):

Small Intestine ◽

Body Temperature ◽

Energy Levels ◽

Liver Weight ◽

The Body ◽

Body Temperatures ◽

Average Surface ◽

Randomized Design ◽

Males And Females ◽

Completely Randomized Design

<p>The objective of this study was to evaluate the effect of different nutritional plans on the body temperature and organ biometrics in male and female broilers, of two ages. Here, 1,700 birds were used (850 males and 850 females) in a completely randomized design composed of five treatments (- 3%, - 1.5%, reference, + 1.5% and + 3%), with 10 repetitions, totaling 50 experimental units; the reference treatment based on nutritional and energy levels indicated in previous studies was calculated from this. At 35 and 42 d, the temperatures of the wing, head, shin, back, and cloaca in males and females were measured separately, and the average surface and body temperature were calculated. At 42 d, relative weights of the gizzard, liver, heart, and small intestine were calculated. The temperatures of the wings, back, and cloaca, and consequently the average surface temperature and body temperatures, were not affected by nutritional plans. Effects of increasing the nutritional and energy levels were observed on liver weights, the gizzard, and the small intestine. We conclude that the nutritional plans did not affect body temperature. Males had higher body temperatures than females. Body temperature increased with increase in age, and the increase in the nutritional plans increased liver weight and reduced the gizzard weights.</p>

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Effect of Storage Temperature on Beauveria bassiana (Bals.) Vuill. Viability on Several Carriers

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v22i1.29 ◽

2006 ◽

Vol 22 (1) ◽

Author(s):

Sri Sukamto

Keyword(s):

Beauveria Bassiana ◽

Storage Temperature ◽

Ultra Violet ◽

Rice Flour ◽

Plant Diseases ◽

Maize Flour ◽

The Third ◽

Randomized Design ◽

Completely Randomized Design ◽

Tapioca Flour

One of the entomopatogenous fungus types commonly observed and showed potency as myco-insectiside is Beauveria bassiana(Bals.) Vuill.In order to support effectiveness and patogenous activity of B. bassiana, it is necessary to add a carrying agent that protects its spores from ultra violet ray. This study aims to investigate the effect of storage temperature on viability of B. Bassianaspores on the carrier material. The observation was carried out in the Laboratory of Plant Diseases, Indonesian Coffee and Cocoa Research Institute. The research was arranged in completely randomized design by three factors. The first factor was carrier (C), that consists of C1 = rice flour, C2 = maize flour and C3 = tapioca flour. The second factor was dosage (D), that consists of D1 = 1 g B. bassiana+ 1 g carrier; D2 = 1 g B. bassiana+ 5 g carrier and D3 = 1 g B. bassiana+ 10 g carrier. The third factor was temperature of the storage (T),that consists of T1 = 5oC; T2 = 23oC and T3 = 29oC. Viability of B. Bassiana spores was examined by observing development of 100 blastopores randomly and determined under light microscope with 400 times magnification. Observation was conducted in two replicates after the spores of B. bassiana were kept in the storage for 2, 4, 8 and 16 weeks. The result showed that by adding 1 g tapioca flour and temperature of storage of 5oC was potentiall in keeping viability of B. bassianaspores at least for 2 months. It was due to that tapioca flour gave better effect than rice and maize flours in keeping the storage and appropriate low temperature. Viability of B. bassianaspores decreased with increasing carrier dosage, temperature and duration of the storage. Whereas, storage at 5oC was found to be a better condition in keeping viability of dry pure B. bassianaspores longer than conditions of 23o and 29oC. Key word:Beauveria bassiana, temperature, viability,carrier.

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Integrated management of tough lovegrass (Eragrostis plana Nees): associating chemical control tools and plant physiology

Ciência Rural ◽

10.1590/0103-8478cr20200271 ◽

2021 ◽

Vol 51 (2) ◽

Author(s):

Eduardo Avelino Faleiro ◽

Diego Martins Chiapinotto ◽

Fabiane Pinto Lamego ◽

Carlos Eduardo Schaedler ◽

Eduardo Bohrer de Azevedo

Keyword(s):

Solar Radiation ◽

Integrated Management ◽

Dry Mass ◽

Radiation Factor ◽

Factor B ◽

Randomized Design ◽

Completely Randomized Design ◽

Factor C ◽

Resource Conditions ◽

And Control

ABSTRACT: The objective of the study was to evaluate the efficiency of herbicides use in the control of tough lovegrass according to the availability of solar radiation and the presence or absence of flooded. Two experiments were conducted in a completely randomized design, in a 2x2x4 and 2x2x5 factorial scheme, where factor A was equivalent to the environment (natural or reduced radiation); factor B to water condition (with a 2 cm flooded or without); and, factor C the herbicides: cyhalofop butyl (315 g ha-1), glyphosate (1080 g ha-1), cyhalofop butyl + glyphosate (315 g ha-1 + 1080 g ha-1) and control (without application) in the first experiment. In the second experiment, the herbicides used were cyhalofop butyl (315 g ha-1), glyphosate (1080 g ha-1), sethoxydim (184g ha-1), imazethapyr (106 g ha-1) and control (without application). Glyphosate efficiently controls plants of tough lovegrass with four tillers (>90%), being superior to cyhalofop, imazethapyr and sethoxydim; independently, of resource conditions. Reduction in the availability of solar radiation generates less shoot dry mass production from the weed, and improves the control only by imazethapyr and cyhalofop. In general, a flooded condition does not affect tough lovegrass control by herbicides.

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Effect of different selenium sources and concentrations on glutathione peroxidase activity and cholesterol metabolism of beef cattle

Journal of Animal Science ◽

10.1093/jas/skab321 ◽

2021 ◽

Author(s):

Janaina S da Silva ◽

Alessandra F Rosa ◽

Cristina T Moncau ◽

Bárbara Silva Vignato ◽

Silvana Marina P Pugine ◽

...

Keyword(s):

Beef Cattle ◽

Cholesterol Metabolism ◽

Control Treatment ◽

Glutathione Peroxidase Activity ◽

Randomized Design ◽

Liver Concentration ◽

Total Meat ◽

Completely Randomized Design ◽

And Control ◽

Different Sources

Abstract The objective of this study was to investigate the effects of different Se sources and concentrations on glutathione forms and cholesterol metabolism in beef cattle. Sixty-three Nellore bulls (412 ± 19 kg BW; 24 months old) were randomly assigned to a completely randomized design in a 2×3 + 1 factorial arrangement (63 pens; one animal/pen) with two Se sources (sodium selenite, ING and Se-yeast, ORG), three concentrations (0.3, 0.9 and 2.7 mg supplemental Se/kg DM), and control treatment (without Se supplementation) fed for 90 days. Blood samples were collected on d 0, 28, 56, and 84. Muscle and liver samples were collected at harvest. Hepatic GSSG (P = 0.004), GSH/GSSG ratio (P = 0.030), and GSH-Px (P = 0.004) were affected by Se source x concentration interaction. Oxidized glutathione was higher in the ORG group vs. ING at concentration 2.7 mg supplemental Se/kg DM, but at 0.3 mg supplemental Se/kg DM the ING group was higher than ORG. The liver GSH-Px activity was higher in the ORG group vs. ING at concentration 0.9 and 2.7 mg supplemental Se/kg DM. The GSH/GSSG ratio was the highest in animals fed 0.3 mg supplemental Se/kg DM of ORG. Selenium liver concentration increased linearly with the supplemental Se concentration in the diet (y = 0.0583 + 0.4254x, R 2 = 0.92, P < 0.0001), regardless of source. Total meat cholesterol was greater (P < 0.001) in CON (control) vs. SUP (supplemented, regardless source) group. The muscle GSH-Px activity was higher (P < 0.001) in SUP vs. CON and increased (P < 0.004) with increasing supplemental Se concentrations. There was an increase on VLDL, glucose, and triglycerides in ORG vs. ING (P ≤ 0.035). In general, serum Se was higher (P < 0.001) in SUP vs. CON and increased with increasing supplemental Se concentration. Lastly, the HMGCR concentration was lower (P = 0.002) in SUP (0.39 ng/mL) vs. CON (0.55 ng/mL). Selenium supplementation with different sources and concentrations has the potential to affect cholesterol metabolism by affecting GSH/GSSG ratio, GSH-Px, and the HMGCR.

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Enhancement of clover growth by inoculation of P-solubilizing fungi and arbuscular mycorrhizal fungi

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652010000300023 ◽

2010 ◽

Vol 82 (3) ◽

pp. 771-777 ◽

Cited By ~ 6

Author(s):

Edson L. Souchie ◽

Rosario Azcón ◽

Jose M. Barea ◽

Eliane M.R. Silva ◽

Orivaldo J. Saggin-Júnior

Keyword(s):

Growth Rate ◽

Aspergillus Niger ◽

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Trifolium Pratense ◽

Arbuscular Mycorrhizal ◽

Randomized Design ◽

Completely Randomized Design ◽

Environment Chamber ◽

And Control

This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L-1 of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger and the yeast Yarowia lipolytica) and control (non-inoculated treatment). The greatest clover growth rate was recorded when Aspergillus niger and PSF 21 were co-inoculated with arbuscular mycorrhizal fungi. Aspergillus niger, PSF 7 and PSF 21 were the most effective isolates on increasing clover growth in the presence of arbuscular mycorrhizal fungi. Greater mycorrhizal colonization resulted in greater clover growth rate in most PSF treatments. PSF 7 was the best isolate to improve the establishment of mycorrhizal and rhizobia symbiosis.

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In vitro antimicrobial activity and alternative control of anthracnose in papaya

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000192018 ◽

2018 ◽

Vol 85 (0) ◽

Author(s):

Héber Ferreira dos Reis ◽

Lilian Maria Arruda Bacchi ◽

Silvana de Paula Quintão Scalon ◽

Jasna Karoliny Pereira Flores

Keyword(s):

Colletotrichum Gloeosporioides ◽

Syzygium Aromaticum ◽

Alternative Control ◽

Ginger Extract ◽

Cinnamomum Zeylanicum ◽

Randomized Design ◽

Completely Randomized Design ◽

And Control

ABSTRACT: We evaluated the efficacy of natural products in the control of papaya anthracnose, in vitro and in vivo. The in vitro experiments for evaluation of mycelial growth used a completely randomized 10 × 4 factorial design (treatments × evaluation periods) with eight replicates, with sporulation evaluated at the end of the experiment. The treatments involved the use of aqueous extract at concentrations of 5 and 15% for Syzygium aromaticum (L.) Merr. & Perry (clove), Cinnamomum zeylanicum Breym (cinnamon), and Zingiber officinalis Rox. (ginger); 1 and 3% chitosan; the fungicide Prochloraz at 100 µg.mL-1; and a control (no treatment). For evaluating conidia germination, we used six treatments with five replicates. The treatments included 7.5% of each extract (clove, cinnamon, and ginger), 1.5% chitosan, and 50 µg.mL-1 of Prochloraz. For the in vivo experiment, “Formosa” papaya “Tainung 1” was used in a completely randomized design, with six treatments and four replicates to evaluate the severity of anthracnose caused by Colletotrichum gloeosporioides. The fruits were treated by immersion for 5 min with 15% clove, cinnamon, and ginger extracts, 8% chitosan, and control with distilled water, and immersion for 2 min in Prochloraz (33.75 g a.i./100 L). The treatments with 15% clove extract and 8% chitosan were effective in all evaluations, resulting in a viable alternative to the fungicide Prochloraz. The treatments with ginger extract were less effective and those with cinnamon offered intermediate control.

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Health quality and germination of Butia capitata seeds treated with bactericide and fungicide

Summa Phytopathologica ◽

10.1590/0100-5405/2128 ◽

2016 ◽

Vol 42 (4) ◽

pp. 303-307

Author(s):

Vander Rocha Lacerda ◽

◽

Diemesson San Tiago Mendes ◽

Fernando da Silva Rocha ◽

Maria de Fátima Gonçalves Fernandes ◽

...

Keyword(s):

Block Design ◽

Transparent Plastic ◽

Randomized Block Design ◽

Randomized Design ◽

Seed Health ◽

High Incidence ◽

Health Quality ◽

Completely Randomized Design ◽

Test Treatments ◽

And Control

ABSTRACT Propagation of “coquinho-azedo” (Butia capitata) is limited due to dormancy and high incidence of fungi in its seeds. The aim of this study was to evaluate the effect of treating “coquinho-azedo” seeds with different concentrations of bactericide and/or fungicide on the incidence of fungi and on the germination of seeds. Thus, B. capitata seeds were immersed in four doses of the bactericide kasugamycin (0, 1.5, 3.0 and 4.5 mL/L) and/or in four concentrations of the fungicide carbendazim+ thiram (0, 2.92, 5.84 e 8.75 mL/Kg), totaling 16 treatments. After the treatments, the operculum was removed from the seeds, which were placed on trays containing autoclaved sand, covered with transparent plastic film and kept in the nursery. In the germination test, treatments were distributed in randomized block design, factorial arrangement of 4x4 (bactericidal doses x fungicide doses), with four replicates of 50 seeds per plot. For the test of seed health quality, the Blotter test was employed in completely randomized design, using 20 replicates and 10 seeds. Health quality and germination were evaluated at 15 and 40 days after the treatment of seeds, respectively. There was no interaction between the bactericide and the fungicide on germination and control of fungi associated with seeds. However, there was an effect of fungicide concentrations on the evaluated parameters. We observed a negative correlation between the concentrations of the fungicide carbendazim + thiram and fungal incidence, and a positive correlation between the fungicide concentrations and seed germination. Therefore, fungicide treatment reduces the fungal incidence and provides better germination of B. capitata seeds.

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