scholarly journals EVALUATION OF EMERGING METHODS ON THE POLYPHENOL CONTENT, ANTIOXIDANT CAPACITY AND QUALITATIVE PRESENCE OF ACETOGENINS IN SOURSOP PULP (Annona muricata L.)

2017 ◽  
Vol 39 (spe) ◽  
Author(s):  
ANDRÉS ELOY LEÓN FERNÁNDEZ ◽  
EVA NOEMÍ OBLEDO-VÁZQUEZ ◽  
MARIA DE LOS ANGELES VIVAR-VERA ◽  
SONIA GUADALUPE SÁYAGO AYERDI ◽  
EFIGENIA MONTALVO-GONZÁLEZ
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Extraction Methods ◽  
Annona Muricata ◽  
Polyphenol Content ◽  
Scavenging Capacity ◽  
Efficient Extraction ◽  
Short Time ◽  
Chloroform Methanol ◽  
Ethyl Acetate Extracts

ABSTRACT The aim of this study was to obtain extracts from soursop pulp that were obtained with different solvents (chloroform, methanol, ethyl acetate and water) and different extraction methods (soxhlet, sonication and microwave), and analysed their extractable polyphenol content, antioxidant capacity and qualitative presence of acetogenins. The most efficient extraction method to obtain extractable polyphenols with high values of scavenging capacity by DPPH was sonication followed by microwave when methanol was used. The acetogenins were detected only in chloroform and ethyl acetate extracts obtained by the three extraction methods. Sonication or microwave was effective to obtain acetogenins or phenolic extracts in greater quantity than reported in soursop pulp, in a short time and few solvent.

scholarly journals Antioxidant and Antimicrobial Activities of the Extracts from Different Garcinia Species

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Nguyen Hoai Nguyen ◽  
Minh Tan Nguyen ◽  
Hiep Dinh Nguyen ◽  
Phuoc Dien Pham ◽  
Ut Dong Thach ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Polyphenol Content ◽  
Gram Positive ◽  
Total Polyphenol ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Total Polyphenol Content

Background. Garcinia is a large genus which has promising bioactivities. However, the properties of many Garcinia species have not been investigated thoroughly. Aim. To determine the antioxidant and antimicrobial capabilities of the extracts from different Garcinia species. Methodology. Six Garcinia species, including Garcinia fusca, Garcinia hopii, Garcinia planchonii, Garcinia nigrolineata, Garcinia gaudichaudii, and Garcinia tinctoria were extracted using n-hexane, ethyl acetate, and methanol, producing n-hexane extract (HE), ethyl acetate extract (EAE), and methanol extract (ME). After that, the total polyphenol content was evaluated using Folin–Ciocalteu assay. DPPH, hydroxyl radical scavenging, and total antioxidant capacity assays were performed to test the antioxidant activity. Subsequently, the antimicrobial activities against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains were assessed using Kirby Bauer and the broth microdilution methods. Results. Many Garcinia extracts contained high total polyphenol content consisting of ME of G. hopii ad G. tinctoria, and EAE of G. planchonii and G. tinctoria. The EAE of G. tinctoria showed effective antioxidant capacity (IC50 = 1.5 µg/mL). Additionally, the EAE of G. gaudichaudii was effective against Gram-positive bacteria with minimal inhibition concentration (MIC) of 15.625–25 µg/mL whereas ME of G. planchonii was effective against both Gram-positive bacteria (MIC = 160 µg/mL) and Gram-negative bacteria (MIC = 75 µg/mL). Conclusion. Several extracts of Garcinia species demonstrated valuable antioxidant and antimicrobial properties.

scholarly journals PHYTOCHEMICAL STUDIES OF ENDOLICHENIC FUNGI ISOLATED FROM HYPOTRACHYNA INFIRMA (KUROK.) HALE

2021 ◽  
pp. 69-73
Author(s):  
GOKILAVANI R. ◽  
H. REHANA BANU
Keyword(s):  
Ethyl Acetate ◽  
Geotrichum Candidum ◽  
Extraction Methods ◽  
Phytochemical Study ◽  
Qualitative And Quantitative ◽  
Endolichenic Fungi ◽  
Phytochemical Studies ◽  
Scytalidium Lignicola ◽  
Nigrospora Oryzae ◽  
Ethyl Acetate Extracts

Objective: The aim of this study is to investigate of phytopharmaceutical importance of endolichenic fungi isolated from Hypotrachyna infirma (Kurok.) Hale. Methods: The lichen species were collected from Sholaiyar hills, Coimbatore and identified as Hypotrachyna infirma (Kurok.)Hale. From this lichen, 29 endolichenic fungi were isolated and 13 endolichenic fungi were identified. From the identified endolichenic fungi, 26 extracts were prepared by successive solvent extraction methods using Ethyl acetate and chloroform. Results: The phytochemical study revealed the presence of important constituents like Alkaloids, Tannins, Carbohydrates, Phenols, Protein, Terpenoids, Steroids, Glycosides Flavonoids and Saponins. From the 13 endolichenic fungi, only 5 endolichenic fungi (Nigrospora oryzae (Berkand Broome)Petch, Geotrichum candidum Link, Scytalidium lignicola pesante, Aspergillus oryzae(Ahlb.) cohn, Aspergillus niger Gr.) have more constitutents. These 5 endolichenic fungi have good results in Quantitative analysis also. Conclusion: Compared to ethyl acetate extracts Chloroform extracts showed very less concentration of the phytochemicals. From this study we conclutated Nigrospora oryzae (Berk and Broome) Petch gave the best results in both qualitative and quantitative compared to other endolichenic fungi.

scholarly journals IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF FRACTIONATED EXTRACTS AND ISOLATION OF BIOACTIVE COMPONENTS FROM BREYNIA VITIS-IDAEA (BURM. F.) C. E. C. FISCHER LEAVES

2018 ◽  
Vol 54 (2C) ◽  
pp. 354
Author(s):  
Nguyễn Thị Lan Hương
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Bioactive Components ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Ethyl Acetate Extracts

This study investigated the antioxidant and antibacterial activities of ethyl acetate, nbutanoland ethanol extracts of Breynia vitis-idaea (Burm.f.) C. E. C. Fischer leaves using invitro assays;and isolated bioactive compounds from the fractioned extract which showed the bestproperties by column chromatography. All extracts showed significant radical scavengingactivities and exhibited antibacterial activities against Enterococcus faecalis, Staphylococcusaureus and methicillin-resistant Staphylococcus aureus (MRSA). Ethyl acetate extracts showedthe highest free radical scavenging capacity with the IC50 values of 99.55 and 94.66 μg/ml (inDPPH and ABTS assays, respectively) and exhibited MIC values of 1.5, 1 and 1 mg/ml againstthe three bacteria, respectively. In addition, from ethyl acetate extracts, one pure compound hasbeen obtained and identified as 6-O-benzoylarbutin.

Assessment of Antimicrobial Activity and total Polyphenol Content of Rosa multiflora and Rosa wichuraiana

2019 ◽  
Vol 10 (04) ◽  
pp. 646-650
Author(s):  
Hyeusoo Kim ◽  
Kyeong Won Yun
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Fraction ◽  
Rosa Multiflora ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Water Fraction ◽  
Total Polyphenol Content ◽  
Rosa Species

The fruit of Rosa multiflora has been used as traditional herbal medicine in Asian countries. The present investigation was undertaken to study the antimicrobial activity and total polyphenol content of hexane, ether, ethyl acetate, water fraction of methanol extract of fruit and flower from Rosa multiflora and Rosa wichuraiana. Antimicrobial activity of the mentioned fractions against 3 gram-positive and 4 gram-negative bacteria using disk diffussion method. The measurement of minimal inhibition concentration (MIC) showed that the ethyl acetate fraction of the two Rosa species is the most effective against the tested bacteria. The total polyphenol content of ethyl acetate fraction of the two Rosa species is higher than the other fractions. The results indicate the antimicrobial activity was related with the total polyphenol content and the fruit and flower of the two Rosa species can be considered as a natural source of antimicrobial agents.

scholarly journals Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2555
Author(s):  
Sang Koo Park ◽  
Yoon Kyung Lee
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Polyphenol Content ◽  
Abts Assay ◽  
Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

scholarly journals The Effect of Mechanical Vibration during Transport under Model Conditions on the Shelf-Life, Quality and Physico-Chemical Parameters of Four Apple Cultivars

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 81
Author(s):  
Dorota Walkowiak-Tomczak ◽  
Natalia Idaszewska ◽  
Grzegorz P. Łysiak ◽  
Krzysztof Bieńczak
Keyword(s):  
Antioxidant Capacity ◽  
Dry Matter ◽  
Mechanical Vibration ◽  
Life Quality ◽  
Titratable Acidity ◽  
Quality Parameters ◽  
Soluble Solids ◽  
Chemical Parameters ◽  
Polyphenol Content ◽  
Flesh Colour

The study assessed the changes in the quality and physical and chemical parameters of apples of four cultivars (‘Gala’, ‘Idared’, ‘Topaz’, ‘Red Prince’) subjected to mechanical vibration during transport under model conditions and after storage (shelf-life). Quality changes in apples were evaluated based on skin and flesh colour, total soluble solids, dry matter, firmness, titratable acidity, pH value, total polyphenol content, and antioxidant capacity. The vibration applied at a frequency of 28 Hz caused changes in the above parameters, which were visible also after storage and depended on the cultivar, but often did not show any clear trend or direction or were not statistically significant. The values of the total colour difference factor ΔE showed considerable variations in the skin colour but only small variations in the flesh colour of individual cultivars. Vibration resulted in a decrease in firmness. Variations in dry matter, total soluble solids, pH, and titratable acidity were small, often insignificant. Mechanical vibration and storage led to an increase in the polyphenol content and antioxidant capacity of all studied cultivars. The greatest stability of quality parameters, relatively high content of bioactive compounds, and antioxidant capacity were observed for ‘Red Prince’. The lowest quality parameters were noted for ‘Gala’. The analysed cultivars continued to show a high level of antioxidant capacity after treatment, which allows the conclusion that they remain a good source of bioactive compounds after transport and short-term storage.

scholarly journals Inferring Long-Term Demand of Newly Established Stations for Expansion Areas in Bike Sharing System

2021 ◽  
Vol 11 (15) ◽  
pp. 6748
Author(s):  
Hsun-Ping Hsieh ◽  
Fandel Lin ◽  
Jiawei Jiang ◽  
Tzu-Ying Kuo ◽  
Yu-En Chang
Keyword(s):  
New York ◽  
Feature Extraction ◽  
Real World ◽  
Extraction Methods ◽  
Real World Data ◽  
Urban Dynamics ◽  
Bike Sharing ◽  
The Government ◽  
Short Time

Research on flourishing public bike-sharing systems has been widely discussed in recent years. In these studies, many existing works focus on accurately predicting individual stations in a short time. This work, therefore, aims to predict long-term bike rental/drop-off demands at given bike station locations in the expansion areas. The real-world bike stations are mainly built-in batches for expansion areas. To address the problem, we propose LDA (Long-Term Demand Advisor), a framework to estimate the long-term characteristics of newly established stations. In LDA, several engineering strategies are proposed to extract discriminative and representative features for long-term demands. Moreover, for original and newly established stations, we propose several feature extraction methods and an algorithm to model the correlations between urban dynamics and long-term demands. Our work is the first to address the long-term demand of new stations, providing the government with a tool to pre-evaluate the bike flow of new stations before deployment; this can avoid wasting resources such as personnel expense or budget. We evaluate real-world data from New York City’s bike-sharing system, and show that our LDA framework outperforms baseline approaches.

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