Hydrogen peroxide and chlorine dioxide against parasite Ichthyophthirius multifiliis (Protozoa, Ciliophora) in jundiá fingerlings

ABSTRACT: Ichthyophthiriasis is a worldwide fish disease with great financial impact on freshwater fish farming due to its associated high mortality rates. Current study assesses the parasiticidal capacity of hydrogen peroxide (H2O2) and chlorine dioxide (ClO2) against the causative agent, Ichthyophthirius multifiliis, in jundiá. Median lethal concentration (LC50, 96h) of each chemical agent was established, as well as the minimum inhibitory concentration of hydrogen peroxide for the parasite´s infectious larval phase (theront). Products were tested asynchronously in parasitized fingerlings for short and long baths at the following concentrations and exposure times: 1. Hydrogen peroxide: (T1) continuous bath - 30ppm and (T2) 50ppm; (T3) short bath - 150ppm, during 1h and (T4) 250ppm during 1h; control group (without any chemical agent). 2. Chlorine dioxide: (T1) continuous bath - 4ppm and (T2) 20ppm; (T3) short bath - 200ppm, during 1min; (T4) short bath - 400ppm, during 1min and control group. Data analysis demonstrated a concentration of 82.54ppm of the commercial product (or 24.76ppm of the active chemical agent) as LC50, 96h of H2O2 and 38.4ppm product (or 2.68ppm of the active chemical agent) for ClO2. Hydrogen peroxide concentration causing 100% mortality rate of theronts in 1h was 25ppm (product, or 7.5ppm of the active chemical agent). At the end of the fourth day of curative experiment, 98% of the animals died by ichthyophthiriasis. No treatment was effective against the parasite.

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Comparative Assessment of Inflammatory Reaction in Experimental Animals after Pleurodesis with Solutions of Hydrogen Peroxide and Talc

International Journal of Biomedicine ◽

10.21103/article11(3)_oa7 ◽

2021 ◽

Vol 11 (3) ◽

pp. 291-295

Author(s):

Mikhail S. Izyumov ◽

Viktor V. Bulynin ◽

Evgeniy S. Ovsyannikov ◽

Andrey M. Bobrovskikh ◽

Anastasia V. Medvedeva ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Free Cell ◽

Control Group ◽

Parietal Pleura ◽

Chemical Agent ◽

Chemical Pleurodesis ◽

Experimental Animals ◽

Comparison Groups ◽

Minimum Number

The aim of our research was to compare the nature and severity of the inflammatory process in the lungs, in the leaves of the visceral and parietal pleura, and in the adjacent subpleural tissues of the chest wall in experimental animals after pleurodesis with solutions of 3% and 6% hydrogen peroxide, and talc. Methods and Results: The experiment was carried out on 200 Wistar rats, weighing 160-180 grams, 10 specimens in a subgroup, depending on the time of the experiment, i.e. 50 specimens in each study group, including the control group. The main criterion by which we determined the comparative characteristics of the effectiveness of talc and 3% and 6% solutions of hydrogen peroxide as preparations used for chemical pleurodesis in the rats was a morphological characteristic of inflammation. This criterion was confirmed by counting free cell populations in lung tissue (lymphocytes, macrophages, neutrophils, histiocytes). All comparison groups were characterized by a gradual increase in the number of lymphocytes, macrophages and histiocytes, ranging from minimum to maximum values, and by a gradual decrease in the number of neutrophils, starting with max and ending with minimum values. The number of lymphocytes, macrophages and histiocytes were increasing faster. But at the same time, for the most part, their number was lower after pleurodesis with 6% hydrogen peroxide. The minimum number of neutrophils and the fastest possible reduction in all cases was observed in pleurodesis with 6% hydrogen peroxide. Conclusion: Pleurodesis with a 6% solution of hydrogen peroxide as a chemical agent significantly affects the quality of the inflammatory response, reducing its duration and severity in the organs and tissues of the rats’ chests, compared with a solution of 3% hydrogen peroxide and talc.

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A Comparison of the Bleaching Effectiveness of Chlorine Dioxide and Hydrogen Peroxide on Dental Composite

Brazilian Dental Journal ◽

10.1590/0103-6440201300098 ◽

2014 ◽

Vol 25 (6) ◽

pp. 524-527 ◽

Cited By ~ 3

Author(s):

Anirudha Agnihotry ◽

Karanjot S. Gill ◽

Deepak Singhal ◽

Zbys Fedorowicz ◽

Sambit Dash ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Chlorine Dioxide ◽

Color Change ◽

Dental Composite ◽

Control Group ◽

Distilled Water ◽

Water Control ◽

Color Differences ◽

Clinically Significant ◽

Office Systems

This study was carried out to verify if composites could be bleached using chlorine dioxide as compared with hydrogen peroxide. 3M ESPE Filtek Z350 Universal Restorative discs were prepared (n=40), with dimensions 5 mm diameter x 2 mm thickness. The discs were divided into 4 groups of 10 discs each. Color assessment was performed by CIEDE2000. The discs were stained with coffee, tea, wine and distilled water (control) solutions for 14 days, 5 hours daily. Color assessment was repeated on stained discs and followed by bleaching of 5 discs from each group using chlorine dioxide and hydrogen peroxide in-office systems. Finally, a last color assessment was performed and compared statistically. DE2000 after bleaching was very close to baseline for both the bleaching agents, although chlorine dioxide showed better results than hydrogen peroxide. After staining, there was a clinically significant discoloration (∆E2000≥3.43) for the tea, coffee and wine groups, and discoloration (∆E2000) was seen more in the wine group as compared to tea and coffee. Overall, the control group (distilled water) had the least color change in the three intervals. After bleaching, the color in all specimens returned close to the baseline. The color differences between bleaching and baseline were less than 3.43 for all groups. The obtained results show that chlorine dioxide is slightly superior to hydrogen peroxide in the bleaching of composites, while maintaining the shade of the composite close to the baseline.

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Comparative Morphological Efficiency Different Methodics of Pleurodesis by Hydrogen Peroxide Solutions and Talc

Vestnik of Experimental and Clinical Surgery ◽

10.18499/2070-478x-2017-10-2-123-129 ◽

2017 ◽

Vol 10 (2) ◽

pp. 123-129 ◽

Cited By ~ 1

Author(s):

Mikhail Sergeevich Izyumov ◽

Viktor Viktorovich Bulynin ◽

Andrew Mikhailovich Bobrovskikh

Keyword(s):

Hydrogen Peroxide ◽

Regression Models ◽

Free Cell ◽

Maximum Efficiency ◽

Control Group ◽

Chemical Agent ◽

Urgent Problem ◽

Chemical Pleurodesis ◽

Laboratory Rats ◽

Chemical Agents

Relevance Currently, the choice of effective and safe means for chemical pleurodesis is an urgent problem in the surgical treatment of spontaneous pneumothorax.Objective The purpose of our research was to compare the effectiveness of various methods of pleurodesis in the experiment based on the macro and microscopic study material.Materials and methods We used laboratory rats of the WISTAR line, weighing 180 grams, 200 individuals in the experiment. They were divided into three experimental groups and a control group of 50 rats each. Spontaneous pneumothorax was simulated on one side of the thorax of rats. One of the chemical agents was used in three experimental groups for carrying out the pleurodesis. The animals were observed and sacrificed in groups on days 3, 5, 7, 10, 30 of the experiment. Next, macro and microscopic changes in the lungs and adjacent tissues were described and compared. Regression models were constructed for the change in the number of free cell populations in the lung tissue during the process of analyzing data.Results and its discussion The use of a 6% solution of hydrogen peroxide causes the formation of persistent pleural adhesions with a shorter duration and severity of the inflammatory process in comparison to the analogues. Regression models of the dynamics of the free cellular composition of the pulmonary stroma were created with the aim of predicting the adhesion process.Conclusions The findings suggest that 6% solution of hydrogen peroxide have the maximum efficiency as a chemical agent for pleurodesis.

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The effects of biocides on the growth of aerophytic green algae (Chlorella sp.) isolated from a cave environment

Archives of Biological Sciences ◽

10.2298/abs210321027n ◽

2021 ◽

pp. 27-27

Author(s):

Natasa Nikolic ◽

Gordana Subakov-Simic ◽

Igor Golic ◽

Sladjana Popovic

Keyword(s):

Hydrogen Peroxide ◽

Green Algae ◽

Chlorine Dioxide ◽

Growth Inhibitor ◽

Control Group ◽

Untreated Control Group ◽

Chl A ◽

Chlorella Sp ◽

Ecological Equilibrium ◽

Cave Environment

Lampenflora communities of bacteria, cyanobacteria, algae, mosses and lichens colonize illuminated show-caves as a consequence of disturbances to the caves? ecological equilibrium. These communities have unesthetic impacts and can cause the biodeterioration of limestone. A 15% hydrogen peroxide solution was proposed for use as an ecological agent for the safe removal of these microorganisms. This study tested the effects of three different biocides (hydrogen peroxide, a commercial solution containing salicylic acid and a product containing chlorine dioxide as the active component), on the growth of green algae (Chlorella sp.), which are frequently encountered in lampenflora communities. Chlorella sp. was treated with the biocides under laboratory condition, and chlorophyll a (Chl a) concentrations were measured 1 h, 3 days, 7 days and 10 days after treatment. The change in Chl a concentration was compared to the untreated control group at each time point. All three biocides prevented the growth of green algae and the product containing chlorine dioxide appeared to be the most effective growth inhibitor. Hydrogen peroxide is known to suppress lampenflora growth in caves, and further studies on other biocides remain necessary to identify a solution that is both ecologically safe and economically feasible.

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Bleaching optimization at WestRock mill in Covington, Virginia

TAPPI Journal ◽

10.32964/tj15.9.581 ◽

2016 ◽

Vol 15 (9) ◽

pp. 581-586 ◽

Cited By ~ 1

Author(s):

RICARDO B. SANTOS ◽

PETER W. HART ◽

DOUGLAS C. PRYKE ◽

JOHN VANDERHEIDE

Keyword(s):

Hydrogen Peroxide ◽

Sodium Hydroxide ◽

Chlorine Dioxide ◽

Capital Expenditures ◽

Equipment Repair ◽

Optimization Program ◽

Hardwood Pulp ◽

Improved Performance

The WestRock mill in Covington, VA, USA, initiated a long term diagnostic and optimization program for all three of its bleaching lines. Benchmarking studies were used to help identify optimization opportunities. Capital expenditures for mixing improvement, filtrate changes, equipment repair, other equipment changes, and species changes were outside the scope of this work. This focus of this paper is the B line, producing southern hardwood pulp in a D(EP)DD sequence at 88% GE brightness. The benchmarking study and optimization work identified the following opportunities for improved performance: nonoptimal addition of caustic and hydrogen peroxide to the (EP) stage, carryover of D0 filtrate to the (EP) stage, and carryover of (EP) filtrate to the D1 stage. As a result of actions the mill undertook to address these opportunities, D0 kappa factor decreased about 5%, sodium hydroxide consumption in the (EP) stage decreased about 35%, chlorine dioxide consumption in the D1 stage decreased about 25%, and overall bleaching cost decreased about 15%.

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The Estimation of prostate specific antigen (PSA) concentrations in patients with prostatitis by fully automated ELISA technique.

Journal of Medical Research and Health Sciences ◽

10.15520/jmrhs.v3i11.279 ◽

2020 ◽

Vol 3 (11) ◽

pp. 1100-1104

Author(s):

Hussein Naeem Aldhaheri ◽

Ihsan Edan AlSaimary ◽

Murtadha Mohammed ALMusafer

Keyword(s):

Personal Information ◽

Receptor Gene ◽

Specific Antigen ◽

Gene Clusters ◽

Control Group ◽

P Value ◽

Group Data ◽

Elisa Technique ◽

And Control

The Aim of this study was to determine Immunogenetic expression of Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. This study shows the effect of PSA level in patients with prostatitis and control group, with P-value <0.0001 therefore the study shows a positive significant between elevated PSA levels and Prostatitis.

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Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.1.23 ◽

2019 ◽

Vol 10 (01) ◽

Author(s):

Eman A. Al-Rekabi ◽

Dheyaa K. Alomer ◽

Rana Talib Al-Muswie ◽

Khalid G. Al-Fartosi

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Drinking Water ◽

Lipid Profile ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Male Rats ◽

Control Group ◽

Very Low Density Lipoprotein ◽

Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

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The Effect of Reflexology on Labor Pain and Length Among Primiparous Women: A Randomized Controlled Trial

International Electronic Journal of Medicine ◽

10.31661/iejm805 ◽

2018 ◽

Vol 7 ◽

Author(s):

Azita Kamjoo

Keyword(s):

Controlled Trial ◽

Intervention Group ◽

Active Phase ◽

Labor Pain ◽

Control Group ◽

Singleton Pregnancy ◽

Group Data ◽

Significant Difference ◽

Labor Length ◽

Primiparous Women

Background: Pain control is considered as the key issue in modern midwifery. Along with medical painkillers, reflexology is viewed as a non-medical and noninvasive method. Hence, we aimed to investigate the effect of reflexology on the intensity of pain and length of labor. Materials and Methods: In this clinical trial, participants included 240 Iranian primiparous women with term and singleton pregnancy. Having a 3-4 cm cervical dilatation once they visited the hospital. Through a convenient sampling method, they were selected and then randomly divided into two groups. In the intervention group, reflexology was performed, and the intensity of pain during the active phase of labor along with the length of labor in the active phase was measured by visual analog scale (VAS) and compared with the control group. Data were analyzed using descriptive statistics, t-test, and Mann-Whitney U-test. Result: Data analysis showed a statistically significant difference between the intensity of pain in the 5-7 and 8-10 cm dilatation in the two groups (P=0.01). Moreover, the labor length in the active phase was found to be significantly shorter in the intervention group (P<0.001). Conclusion: It appears that reflexology can lead to a reduction in the pain and length of labor. Therefore, through instructing this technique, a goal of midwifery, which is reducing labor pain and its length can be achieved. Keywords: Reflexology, Labor Pain, Labor Length, Primiparous Women

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Antimicrobial Effect of 940 nm Diode Laser based on Photolysis of Hydrogen Peroxide in the Treatment of Periodontal Disease

Revista de Chimie ◽

10.37358/rc.18.8.6478 ◽

2018 ◽

Vol 69 (8) ◽

pp. 2081-2088 ◽

Cited By ~ 1

Author(s):

Alin Alexandru Odor ◽

Edwin Sever Bechir ◽

Deborah Violant ◽

Victoria Badea

Keyword(s):

Hydrogen Peroxide ◽

Laser Therapy ◽

Diode Laser ◽

Antimicrobial Effect ◽

The Other ◽

Control Group ◽

Group 4 ◽

Periodontal Bacteria ◽

Severe Periodontitis ◽

Before And After

Moderate and severe periodontitis represents a challenge in the non-surgical periodontal therapy. Due to the lack of evidence regarding the antimicrobial effectiveness of 940 nm diode laser in periodontal treatment, this study aimed to evaluate the antimicrobial effect of hydrogen peroxide (H2O2) photolysis performed with 940 nm diode laser in the treatment of moderate and severe periodontitis. Twenty-five patients with 100 teeth were selected for this pilot study. The test teeth were randomly assigned to one of the four treatment groups: Group 1: scaling and root planning (SRP) (control group); and the following experimental groups: Group 2: H2O2; Group 3: 940 nm diode laser therapy; Group 4: 940 nm diode laser therapy and H2O2. Clinical examinations, like probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were performed before and after the treatment. The microbiological evaluation, effectuated before and after the treatment, included nine periodontal bacteria species and investigated by means of real-time PCR assay. The clinical and bacterial differences in the tested groups, was assessed between control group and the other three experimental groups, as well as between the experimental groups. The total bacteria load was reduced for all four studied groups. Group 4 (diode laser + H2O2) showed significant bacterial reduction of the major periodontal bacteria like Pg., Tf., Td., Pi., Pm., Fn (p[0.001) than the other 3 groups (p]0.001). Also the periodontal clinical parameters, like PD, CAL and BOP showed a significant reduction after the photolysis of H2O2 with the 940 nm diode laser (p[0.001). Differences between tested groups showed a significant beneficial results in regard to Group 4.It is suggested that the photoactivation of H2O2 with the 940 nm diode laser can be used successfully in adjunctive to the non-surgical periodontal treatment as a bactericidal tool.

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A Comparative Evaluation of Nanohydroxyapatite-Enriched Hydrogen Peroxide Home Bleaching System on Color, Hardness and Microstructure of Dental Enamel

Materials ◽

10.3390/ma14113072 ◽

2021 ◽

Vol 14 (11) ◽

pp. 3072

Author(s):

Riccardo Monterubbianesi ◽

Vincenzo Tosco ◽

Tiziano Bellezze ◽

Giampaolo Giuliani ◽

Mutlu Özcan ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Particle Diameter ◽

Dental Enamel ◽

Sem Analysis ◽

Control Group ◽

Average Particle ◽

Color Analysis ◽

Enamel Microstructure ◽

Prismatic Structure ◽

20 Nm

This study aimed to evaluate two hydrogen peroxide (HP)-based at-home bleaching systems in order to analyze whether nano-hydroxyapatite (nHA) addition may represent a reliable and safe solution for tooth whitening without altering dental microstructure and hardness. Human third molars (N = 15) were treated with two bleaching agents, one containing 6%HP (6HP) and the other 6% HP nHA-enriched (6HP-nHA) with average particle diameter ranging from 5–20 nm. Their effects on enamel were assessed using a spectrophotometer, Vickers microhardness (VMH) test and Scanning Electron Microscopy (SEM), comparing the treated groups with the non-treated control group (CTR). Color analysis revealed improvement in whiteness in both groups compared to CTR. VMH test results showed no differences among the groups. SEM analysis highlighted no evident changes in the enamel microstructure of tested groups compared to CTR. At high magnification, in 6HP group, a slight increase in irregularities of enamel surface morphology was observed, while 6HP-nHA group displayed removal of the aprismatic layer but preservation of the intact prismatic structure. These results suggest that the 6HP-nHA agent may be recommended to provide reliable whitening treatment, without damaging the enamel micromorphology and hardness.

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