scholarly journals Horses fed meal contaminated with mycotoxins performing short-term exercises

2020 ◽  
Vol 40 (9) ◽  
pp. 717-725
Author(s):  
Auro César Braga ◽  
Fernando Q. Almeida ◽  
Vinicius P. Silva ◽  
Maria I.V. Almeida ◽  
Kelly M. Keller ◽  
...  
Keyword(s):  
Exercise Test ◽  
Interval Training ◽  
Basal Diet ◽  
Serum Biochemistry ◽  
Blood Parameters ◽  
Warm Up ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hematological And Biochemical Parameters ◽  
Experimental Stage

ABSTRACT: This research was carried out to evaluate the effect of mycotoxins on the performance of horses through physiological parameters, and hematology and serum biochemistry analyses. The essay lasted 40 days, with 12 days for adaptation and 28 days of experimentation. In the experimental stage, the horses were distributed in a completely randomized design, with three treatments with four animals each. The treatments used were 0 (control), 50 ppb and 100 ppb of Aflatoxin B1 (AFB1) added to a concentrate in a basal diet. The basal diet contained mycotoxins from feedstuffs naturally contaminated. The exercise test was performed over the 21th day of the experimental stage. The exercise consisted in an interval training test with a warm-up of 17 mins at a trot followed by three gallops of 450m/min. The heart rate was monitored between the gallops. Before the exercise test and immediately after the third gallop, the physiological and blood parameters were evaluated, and continued up to 48 hours after the exercise. The results of the physiological, hematological and biochemical parameters were submitted to analysis of variance (ANOVA) and compared by the Tukey test at 5% of significance. The presence of AFB1 in the diet influenced the alkaline phosphatase activity, which presented higher values in horses fed diet with inclusion of 100 ppb AFB1, suggesting a hepatotoxic activity associated with the others mycotoxins naturally present in the feedstuffs.

scholarly journals Alternatives to the use of antibiotics for laying hens in growing phase

2020 ◽  
Vol 50 (4) ◽  
pp. 553-563
Author(s):  
S.M.C. Tfaile ◽  
J.E. De Moraes ◽  
F.E.L. Budiño ◽  
D.F. Soares ◽  
N.Y. Sitanaka ◽  
...  
Keyword(s):  
Laying Hens ◽  
Immunological Response ◽  
Basal Diet ◽  
Experimental Period ◽  
Serum Biochemistry ◽  
Rapid Decomposition ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Microbiological Analyses ◽  
Antibiotic Residue

This study evaluated the performance, immune response to vaccination, digestive organs, intestinal histomorphometry, and microbiological development in the diet and litter of Isa Brown strains at growing receiving phase alternative additives in the place of antibiotics. Four treatments were arrayed in a completely randomized design: T1) Basal diet without antibiotics, T2) Basal diet with antibiotics, T3) Basal diet with probiotics, and T4) Basal diet without antibiotics and water modified by a photoelectron generator. There were six replications of eight birds each and thus a total of 192 birds. Performance was evaluated for eight weeks and during this period blood samples were collected at 9, 12 and 15 weeks of age for serological evaluation. At the end of the experimental period, 24 birds were euthanized, collecting the intestinal tract and later performing histomorphometry. Microbiological analyses were performed in the experimental diets and in the material of the litter of the boxes. The results obtained were analyzed by analysis of variance and the contrast between treatment means when significant by Tukey’s test. The results indicate that the replacement of antibiotics with T3 or T4 did not interfere negatively (P >0.05) in the performance of the birds, immunological response, viscera analyses and intestinal histomorphometry. In addition, use of T3 and T4 might lead to more rapid decomposition of excreta and reduce antibiotic residue in the soil when the litter is used in crop production.Keywords: dileka, Gallus domesticus, histomorphometry, laying hens, probiotics, serum biochemistry, water

scholarly journals Effect of aflatoxin B1 on digestibility and blood parameters in horses

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Auro Braga ◽  
Fernando Queiroz de Almeida ◽  
Vinicius Pimentel Silva ◽  
Kelly Moura Keller ◽  
Marina Prado Maciel ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Basal Diet ◽  
Experimental Period ◽  
Blood Parameters ◽  
Adaptation Period ◽  
Blood Cell Count ◽  
Collection Method ◽  
Blood Samples ◽  
Randomized Design ◽  
Completely Randomized Design

The aim of this research was to evaluate the effect of mycotoxins on the digestion of nutrients and on the hematological profile of horses. Twelve horses were used in a completely randomized design, with three treatments and four replicates. Aliquots of 50 or 100 µg kg-1 of aflatoxin B1 (AFB1) were added to a basal diet naturally containing mycotoxins. The basal diet did not contain AFB1. The 40-day evaluation included a 12-day adaptation period and a 28-day experimental period. A digestion test was carried out at the end of the experimental period, using the partial stool collection method, using LIPE® as an indicator. Blood samples were collected once a week during 4 weeks of the essay for hematological and biochemical evaluations. The amounts of mycotoxins added did not influence the intake and digestibility of the nutrients (P>0.05). The aflatoxins in the diet influenced the white blood cell count, especially mature neutrophils and the creatine kinase and alkaline phosphatase (P <0.05), which had higher activity in horses fed diets with greater toxicity.  

Changes in Productive Performance, Blood Metabolites and Hematological Parameters of Growing Lambs Supplemented with Two Sources of Choline

2021 ◽  
Author(s):  
J.A. Martínez-García ◽  
J.C. Garcia-Lopez ◽  
P.A. Hernández-García ◽  
G.D. Mendoza-Martínez ◽  
A. Vázquez-Valladolid ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Live Weight ◽  
Basal Diet ◽  
Blood Metabolites ◽  
Metabolic Functions ◽  
Randomized Design ◽  
Dark Color ◽  
Completely Randomized Design ◽  
Oral Doses ◽  
Daily Gain

Background: Choline is a nutrient with numerous metabolic functions, but its requirements for ruminants are unknown. The supplementation with bypass choline could enhance productivity. Methods: Twenty-four male lambs (Rambouillet 23.5 kg±3.17 kg initial BW) were fed a basal diet with treatments which consisted of a control and oral doses of ruminally-protected choline (4 g/d RPC) and plant-based choline (4 g/d Biocholine) in a completely randomized design with initial weight as a covariate. The experiment was conducted for 42 days during which live weight, dry matter intake, carcass characteristics, blood metabolites and basic hemograms were measured. Result: The daily gain in lambs was similar between treatments. Intake was higher in lambs given Biocholine (1.32 kg/d). The L* (represents the light to dark color) value and mineral content in the meat were improved with both sources of choline. Blood triglycerides increased by RPC compared with the other treatments and cholesterol was reduced by Biocholine. Alanine transaminase (ALT) and aspartate aminotransferase (AST) activity decreased by effect of choline. Hematological parameters were affected by choline supplementation regardless of the source; erythrocyte, monocytes and lymphocytes count decreased with both sources of choline in growing lambs.

scholarly journals Digestibility and bioavailability of phosphorus from spray-dried yeasts in the diet of starting pigs (15-30 kg)

2013 ◽  
Vol 14 (2) ◽  
pp. 308-321
Author(s):  
Lina Maria Peñuela Sierra ◽  
Ivan Moreira ◽  
Antonio Claudio Furlan ◽  
Paulo Levi de Oliveira Carvalho ◽  
Ângela Rocio Poveda Parra ◽  
...  
Keyword(s):  
Live Weight ◽  
Basal Diet ◽  
Relative Bioavailability ◽  
Mean Value ◽  
Experimental Unit ◽  
Available Phosphorus ◽  
Standard Curve ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Spray Dried

Two experiments were carried out to determine the bioavailability of phosphorus in two spray-dried yeasts - sugarcane yeast (SCY), and sugarcane yeast + brewer's yeast (SCBY) - in starting pigs, by comparing different methods (Apparent Digestibility Coefficient of Phosphorus - ADCP; True Digestibility Coefficient of Phosphorus - TDCP; slope ratio; and standard curve). In experiment I, a digestibility assay were carried out using 30 cross breed pig with initial weigh of 22.69 ± 4.24kg, allotted in a completely randomized design. The mean ADCP and TDCP values were 62.68 and 64.15% for SCY and 77.01 and 79.33% for SCBY. ADCP and TDCP for SCBY were higher (P<0.05) than the values for SCY. In Experiment II, a growth test was conducted, 56 crossbred piglets, were utilized, with initial live weight of 15.11 ± 3.43kg, allotted in a completely randomized design, with seven treatments, four replications, and two pigs per experimental unit. The treatments consisted of a basal diet without supplementation with P and the same diet including supplementation with two levels of P (0.053% and 0.105%) from dicalcium phosphate, SCY and SCBY. The relative bioavailability mean value of 57.23% for SCY and 91.96% for SCBY, corresponding to 0.30% and 0.40% of available phosphorus, respectively.

scholarly journals Eksplorasi Rizobakteri Indigenous Dan Uji Antagonis Terhadap Patogen Rigidoporus microporus Dan Phellinus noxius Pada Tanaman Karet (Hevea brasiliensis) Secara In Vitro

2020 ◽  
Vol 3 (4) ◽  
pp. 43-52
Author(s):  
Murtadha Murtadha ◽  
M. Abduh Ulim ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Plant Growth ◽  
Hevea Brasiliensis ◽  
Growth Promoter ◽  
Randomized Design ◽  
Pathogen Control ◽  
Completely Randomized Design ◽  
Experimental Stage ◽  
Phellinus Noxius ◽  
Banda Aceh

Abstrak.  Penelitian bertujuaan untuk mendapatkan isolat rizobakteri yang mampu berperan sebagai agens biokontrol terhadap pengendalian patogen R. microporus dan P. noxius secara in vitro serta sebagai agen rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih Jurusan Agroteknologi, Fakultas Pertanian, Universitas Syiah Kuala, Darussalam Banda Aceh, mulai Oktober sampai Desember 2017. Penelitian ini menggunakan Rancangan Acak Lengkap non faktorial. Faktor yang diteliti yaitu isolat rizobakteri, taraf yang dicobakan terdiri dari 15 isolat dan dua patogen antagonis R. microporus dan P. noxius, yang diulang sebanyak 3 kali sehingga terdapat 90 unit satuan percobaan. Hasil penelitian menunujukkan isolat rizobakteri mampu menekan pertumbuhan koloni cendawan patogen. Pada patogen uji R. microporus terdapat 3 rizobakteri yang paling baik yaitu isolat DLG5/3 dengan persentase penghambatan 68,33%, DLG4/1 dengan persentase penghambatan 66,66% dan DLG4/7 dengan persentase penghambatan 63,33%. Pada patogen uji P.noxius terdapat dua rizobakteri yang paling baik dalam menghambat pertumbuhan koloni cendawan patogen yaitu isolat DLG5/1 dengan persentase penghambatan 60,33%, dan DKP6/3 dengan persentase penghambatan 52,50%. Pada laju penghambatan isolat rizobakteri yang paling baik pada patogen R. microporus yaitu isolat DLG6/4 dan DKP4/1 dengan nilai rerata laju penghambatan 20,33 mm/hari. Pada patogen P. noxius menunjukan isolat rizobakteri yang paling baik yaitu isolat DLG4/1 dengan nilai laju penghambatan 12,05 mm/hari.Exploration of Indigenous Rizobacteria and Antagonistic Test against Patogen Rigidoporus microporus And Phellinus noxius In Rubber Plant (Hevea brasiliensis) In VitroAbstract. The research was conducted to obtain rhizobacteria isolates capable of acting as biocontrol agents on pathogen control of R. microporus and P. noxius in vitro and as plant growth promoter rhizobacteria (PGPR). The research was conducted at the Science and Technology of seed Laboratory, Department of Agrotechnology, Faculty of Agriculture, Syiah Kuala University, Darussalam Banda Aceh, starts from October to December 2017. The research using Completely Randomized Design non factorial. Factors researched were rhizobacteria isolates, the experimental stage consisted of 15 isolates and 2 antagonist were R. microporus dan P. noxius, and 3 time repeated until be found 90 units of treatment. The results showed that rhizobacteria isolates capable to inhibith growth of colonies pathogenic. In the pathogen of R. microporus test, there are 3 better rhizobacteria were DLG5/3 isolate with 68.33% inhibition percentage, DLG4/1 with 66.66% inhibition percentage and DLG4/7 with 63,33% inhibition percentage. In the pathogen of P. noxius there are 2 best rhizobacteria inhibiting growth, DLG5/1 isolate with 60.33% inhibition percentage, and DKP6/3 with 52,50% inhibition percentage. In the pathogen of R. microporus test, there are 2 better rhizobacteria were DLG6/4 and DKP4/1 with 20,33 mm/day inhibition percentage. In the pathogen of P. noxius test,  the best rhizobacteria were DLG4/1  with 12,05 mm/day inhibition percentage. 

scholarly journals Semi purified glycerins in growing and finishing pigs feeding (30-90 kg)

2014 ◽  
Vol 15 (1) ◽  
pp. 221-236 ◽  
Author(s):  
Liliane Maria Piano Gonçalves ◽  
Ivan Moreira ◽  
Paulo Cesar Pozza ◽  
Paulo Levi de Oliveira Carvalho ◽  
Juliana Beatriz Toledo ◽  
...  
Keyword(s):  
Vegetable Oil ◽  
Control Diet ◽  
Carcass Traits ◽  
Basal Diet ◽  
Metabolizable Energy ◽  
Experimental Unit ◽  
Finishing Pigs ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Performance Results

Two experiments were carried out to determine the nutritional value and to evaluate the performance and carcass traits in the growing-finishing pigs fed on two types of semipurified glycerin, which were made from vegetable oil (SPGV) and mixed of animal + vegetable oil (SPGM). In the digestibility trial (experiment I) 32 crossbreed barrows were used with initial body weight of 45.08 ± 4.11kg. It were used three levels of inclusion (6, 12 e 18%) of semipurified glycerin (SPGV and SPGM) in the basal diet. The metabolizable energy (ME) value of glycerin were estimated by regression of ME (kcal/kg) intake associated with glycerin vs. glycerin intake (kg). The values of ME (kcal/kg) obtained were: 2,731 for SPGV and 2,210 for SPGM. In experiment II, 72 pigs (30.08 ± 1.65 to 60.58 ± 3.57) in growing and (60.83 ± 2.63 to 93.79 ± 5.72) in finishing phase, were allotted in a completely randomized design in a 2 x 4 + 1 factorial scheme, with two types of semipurified glycerin (SPGV and SPGA), four inclusion levels (4; 8; 12 and 16%), eight experimental unit and a control diet containing no glycerin (0%). All pigs were slaughtered to evaluate the carcass traits. The performance results suggest that it is feasible to use up to 16% of both semipurified glycerins on growing and finishing pigs feeding, without impairing performance and carcass traits.

scholarly journals Hematological, Serum Biochemistry Profile and Nutrient Retention of Finisher Broilers Fed Diet in Which Bio-fermented Rice Husk Meal Substituted Brewer’s Dried Grain

2019 ◽  
Vol 11 (3) ◽  
pp. 465
Author(s):  
J. N. Ikpe ◽  
E. C. Oko ◽  
I. C. Vining-Ogu
Keyword(s):  
Rice Husk ◽  
Serum Cholesterol Level ◽  
Nutrient Retention ◽  
Serum Biochemistry ◽  
Blood Profile ◽  
Randomized Design ◽  
Negative Effect ◽  
The Difference ◽  
Hematological And Biochemical Parameters ◽  
Blood Profiles

Heaps of rice husk waste is currently increasing around rice mills in Nigeria. Production cost for broilers has risen tremendously due to high cost of feed ingredients, one of which is Brewer&rsquo;s Dried Grain (BDG). Rice husk has been identified as a potential feed stuff and can replace BDG in broiler production. The research determined the effect of substituting BDG with bio-fermented rice husk meal on the blood profiles and nutrient retention of the broilers. Four treatment diets were formulated. T1 (control) contained 15% BDG while in T2, T3 and T4 bio-fermented rice husk meal replaced BDG at 33.33%, 66.67% and 100% to be 5%, 10% and 15% respectively. The diets were assigned to 120 broilers of 28 days old in a complete randomized design of 10 broilers replicated 3 times. The broilers were reared for 28 days after which blood samples were collected. Nutrient retention of the diets was also analyzed. There was a significant (P &lt; 0.05) reduction in the WBC, MCH, MCHC and serum cholesterol level of broilers on the test ingredient. Nutrient retention showed a significant (P &lt; 0.05) increase in ash while the control and T2 were similar (P &gt; 0.05) in crude protein. The research discovered that though there were significant results in most the hematological and biochemical parameters, but the trend of the results was not suggestive that the difference was due to diet. It was concluded that bio-fermented rice husk meal may not have a negative effect on the blood profile of finisher broilers.

scholarly journals EFFECTS OF Citrus hystryx AS FAT PROTECTOR ON UNSATURATED FATTY ACIDS, CHOLESTEROL AND CHEMICAL COMPOSITION OF LAMB MEAT

2016 ◽  
Vol 41 (1) ◽  
pp. 45-49 ◽  
Author(s):  
N.C. Tiven ◽  
I. P. Siwa ◽  
L. Joris
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Chemical Composition ◽  
Unsaturated Fatty Acids ◽  
Basal Diet ◽  
Cooking Oil ◽  
Lamb Meat ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test

This study was conducted to determine the effect of Citrus hystrix as fat protector on unsaturated fatty acids, cholesterol and chemical composition of lamb meat. The research design applied was completely randomized design with 3 treatments and 5 replications. Fifteen local male lambs aged 9-12 months weighing 14-17 kg, were divided into 3 groups of different diet treatments (P0 : sheeps were only given a basal diet; P1 : sheeps are given basal diet and 3% cooking oil; P2 : sheeps are given basal diet, 3% cooking oil and protected by 3% Citrus hystrix powder). The data were analyzed by analysis of variance, the differences among treatments were tested by Duncan’s New Multiple Range Test. The results showed meat from sheeps that consume P2 diet has a total fatty acid and polyunsaturated fatty acids content were higher (P

scholarly journals Pemberian Kulit Biji Kopi dalam Ransum Sapi Aceh Terhadap Kecernaan secara In Vitro

2013 ◽  
Vol 13 (1) ◽  
pp. 49-52
Author(s):  
Yunasri Usman ◽  
M. Nur Husin ◽  
Riantia Ratni
Keyword(s):  
Dry Matter ◽  
Animal Husbandry ◽  
Basal Diet ◽  
Animal Nutrition ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Coffee Beans ◽  
Completely Randomized Design

The supplementation peel coffee beans in the ration Aceh cattle on in vitro digestibilityABSTRACT. This research was carried out at the Laboratory of  Feed and Animal Nutrition,  Department of Animal Husbandry Faculty of Agriculture Syiah Kuala University for  73 days from December 26, 2011 to March 7, 2012. The purpose of this experiment was to find out the effects of the coffee peeling on in vitro digestibility of Aceh beef cattle. Completely Randomized Design (CRD) was applied in this experiment consisting of 4 treatments  namely levels of  coffee peeling on basal diet   (R0=0%, R1=10%, R2=20%, R3=30%). Each treatment consists of 5 replications. Parameters observed in this research were dry and organic matter digestibility. The results showed that dry matter digestibility was not significantly different (P0,05)amongst treatments  54,42%, 49,32%, 45,97%, 42,08%) for R0, R1, R2 and R3 respectively.  On the other hand, organic metter digestibility was significantly different (P0,05) in which R2 treatment was the highest and R0 treatment was the lowest (54,59%).

scholarly journals Use of different combinations of enzyme complexes in broiler diets

2021 ◽  
Vol 42 (5) ◽  
pp. 2903-2924
Author(s):  
Lindolfo Dorcino dos Santos Neto ◽  
◽  
Julyana Machado da Silva Martins ◽  
Genilson Bezerra de Carvalho ◽  
Roberto Moraes Jardim Filho ◽  
...  
Keyword(s):  
Basal Diet ◽  
Experimental Period ◽  
Nutrient Digestibility ◽  
Enzyme Complex ◽  
Ether Extract ◽  
Feed Conversion ◽  
Randomized Design ◽  
Final Weight ◽  
Completely Randomized Design ◽  
Enzyme Complexes

Two experiments were carried out to investigate the effect of “on top” addition of different enzyme complexes, the enzyme α-galactosidase and three sources of the enzyme phytase available on the market, in broiler diets. In the first experiment, 1260 one-day-old Cobb 500® chicks were distributed into seven treatments in a completely randomized design (CRD) with six replicates and 30 birds/replicate. Treatments consisted of combinations of different enzyme complexes, namely, complex A (phytase, protease, xylanase, ß-glucanase, cellulase, amylase, pectinase), complex B (protease and cellulase) and complex C (xylanase, amylase and protease); isolated α-galactosidase (GAL); and three sources of phytase (P1, P2 and P3) in the diet. The treatments were formulated as follows: T1 - basal diet (BD); T2 - BD + enzyme complex A + enzyme complex B (BDAB); T3 - BDAB + GAL; T4 - BD + complex A + GAL; T5 - BD + complex C + P1 + GAL (BDCG); T6 - BDCG + P2; and T7 - BDCG + P3. The following variables were measured in the experimental period of 42 days: feed intake (FI), weight gain (WG), average final weight (AFW), feed conversion (FC), and carcass yield. Significant differences occurred for AFW, WG and FC in the pre-starter phase. In the second experiment, 112 Cobb 500® chicks aged 25 days were distributed into seven treatments in a CRD with four replicates and four birds/replicate. Treatments were the same as in the first experiment. Nutrient digestibility was evaluated in an experimental period of seven days. Differences were found in the metabolism coefficient of ether extract (MCEE). Dietary inclusion of enzyme complexes improves the AFW and WG of chickens from 1 to 7 days of age and MCEE in the grower phase.

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