Liver and serum soluble protein changes and pathomorphology in undernourished mice with acute Schistosomiasis mansoni

Body, liver and spleen weights; histopathology of the liver, spleen and intestines; hepatic and serum soluble proteins changes were the parameters studied in undernourished Swiss albino mice experimentally infected with S. mansoni. Non-infected deficient animab had lower liver/body weight and spleen/body weight ratios as compared to the controls (22.60% casein group). Infected mice showed higher values regardless the type of diet. Undernourished infected subgroup showed a persistent exudative periovular reaction in the liver. Soluble hepatic proteins content and serum protein fractions appeared to be lower in the deficient infected mice. A significant difference was detected in the gammaglobulin fraction between infected and non-infected animals fed the control diet with higher values for the former. Our data suggest that the effects of malnutrition, per se, are sometimes more detrimental to the host than those due to Manson 's schistosomiasis.

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Effects of plantago major extract on serum protein fractions in broiler diet

Indian Journal of Animal Research ◽

10.18805/ijar.9641 ◽

2016 ◽

Cited By ~ 1

Author(s):

Nuriye Tugba Bingol ◽

Semiha Dede ◽

Mehmet Akif Karsli ◽

Orhan Yilmaz ◽

Idris Turel ◽

...

Keyword(s):

Serum Protein ◽

Control Diet ◽

Water Extract ◽

Basal Diet ◽

Protein Fractions ◽

Control Group ◽

Broiler Chicks ◽

Protein Levels ◽

Significant Difference ◽

G Ratio

The aim of this study was to determine the effects of Plantago major’s (P.major) water extract added into broiler diets at different levels on serum protein fractions. A total of 112 Ross 308 broiler chicks were used in the study. Experiment consisted of control and 3 treatment groups with a 28 chicks within each group. Each experimental group was divided into four subgroups consisting of 4 chicks. A basal (control) diet was prepared and three experimental diets were established by addition of P.major into basal diet; P.major 1 (5 g/kg feed), P.major 2 (10 g/kg feed), P.major 3 (15 g/kg feed). Broiler chicks were fed with these diets for 42 days ad libitum. Total protein levels of P.major 3 group were lower than other groups and controls. It was determined that the albumin percentages and levels became decreased in a significant ratio in the P.major 1 and P.major 2, (P<0,05), Alpha 1 globulin percentage and concentration were found significantly high in P.major 2 group than the other groups (P<0.05), Alpha 2 level and percentage in P.major 1 group was seen significantly higher than the control group, the A/G ratio in P.major 1 and P.major 2 was seen significantly lower than the control group. There was no significant difference between groups for beta and gamma globulins as percentage.

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Efektivitas Vaksinasi Crude dan Soluble Protein Spora Myxobolus Koi terhadap Tingkat Kerusakan Usus Ikan Koi (Cyprinus carpio Koi) [ The Effectivety Crude and Soluble Protein of Myxobolus Koi Spore againts Intestine Different Degrees in Koi (Cyprinus carpio Koi)]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v7i1.11233 ◽

2019 ◽

Vol 7 (1) ◽

pp. 55

Author(s):

Rachma Woro Anggarani ◽

Gunanti Mahasri ◽

Lucia Tri Suwanti

Keyword(s):

Molecular Weight ◽

Statistical Analysis ◽

Cyprinus Carpio ◽

Soluble Protein ◽

Crude Protein ◽

Soluble Proteins ◽

Test Results ◽

Group I ◽

Significant Difference ◽

Cyprinus Carpio Koi

Abstract Myxobolus koi is one species of Myxobolus sp that can cause parasitic diseases in fish called Myxobolusis. Based on the Decree of the Minister of Marine and Fisheries No: KEP.03/MEN/2010 that Myxobolus koi in the list of Fish Quarantine Pests group I. Myxosporean diseases are most numerous in the water can cause Proliferative Kidney Disease (PKD) and Whirling Disease (WD). The aim of this research is to finding, analyzing and determining the protein of spores Myxobolus koi that can effectively reduce the level of damage to the intestinal organs as well as for the prevention myxobolusis on Koi’s. Then for finding, analyzing and determining the protein of spores Myxobolus koi do isolation of spore proteins. The study consisted of three phases examination to preparation and identification of spores, isolation and analyze of crude and soluble protein spores for obtain dose and molecular weight each protein and histopathological test. This research uses descriptive method. The data presented may be narratives, images, tables or charts for each group. Intestinal histopathology test results of research carried scoring Koi’s were analyzed using the Kruskal-Wallis. The results showed a profile crude protein and soluble proteins from spores Myxobolus koi showed that the molecular weight of crude protein Myxobolus koi in this study was 150 kDa and 72 kDa and for soluble protein was 73 kDa. Results scoring the degree of infection caused by exposure to Myxobolus koi then statistically processed with an average yield of scoring in a sequence of 0; 1.6; 0.64 and 0.32. Statistical analysis showed no significant difference in the treatment of K + with crude protein, and K + with soluble proteins. Statistical analysis showed that there were significant differences in treatment with K+ and K-, K- with soluble protein and crude protein and soluble protein. Histopathological changes in the intestine in the form of inflammatory cell infiltration, necrosis and haemorage

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Different Responses to a High-Fat Diet in IL-6 Conditional Knockout Mice Driven by Constitutive GFAP-Cre and Synapsin 1-Cre Expression

Neuroendocrinology ◽

10.1159/000496845 ◽

2019 ◽

Vol 109 (2) ◽

pp. 113-130 ◽

Cited By ~ 9

Author(s):

Olaya Fernández-Gayol ◽

Paula Sanchis ◽

Kevin Aguilar ◽

Alicia Navarro-Sempere ◽

Gemma Comes ◽

...

Keyword(s):

Body Weight ◽

High Fat Diet ◽

Control Diet ◽

Conditional Knockout ◽

Mrna Levels ◽

High Fat ◽

Significant Difference ◽

Cellular Source ◽

The Central Nervous System ◽

Synapsin 1

Background/Aims: Interleukin-6 (IL-6) is a major cytokine controlling body weight and metabolism, at least in part through actions in the central nervous system (CNS) from local sources. Methods: We herewith report results obtained in conditional IL-6 KO mice for brain cells (Il6ΔGfap and Il6ΔSyn). Results: The reporter RiboTag mouse line demonstrated specific astrocytic expression of GFAP-dependent Cre in the hypothalamus but not in other brain areas, whereas that of synapsin 1-dependent Cre was specific for neurons. Feeding a high-fat diet (HFD) or a control diet showed that Il6ΔGfap and Il6ΔSyn mice were more prone and resistant, respectively, to HFD-induced obesity. Energy intake was not altered in HFD experiments, but it was reduced in Il6ΔSyn male mice following a 24-h fast. HFD increased circulating insulin, leptin, and cholesterol levels, decreased triglycerides, and caused impaired responses to the insulin and glucose tolerance tests. In Il6ΔGfap mice, the only significant difference observed was an increase in insulin levels of females, whereas in Il6ΔSyn mice the effects of HFD were decreased. Hypothalamic Agrp expression was significantly decreased by HFD, further decreased in Il6ΔGfap, and increased in Il6ΔSyn female mice. Hypothalamic Il-6 mRNA levels were not decreased in Il6ΔSyn mice and even increased in Il6ΔGfapmale mice. Microarray analysis of hypothalamic RNA showed that female Il6ΔGfap mice had increased interferon-related pathways and affected processes in behavior, modulation of chemical synaptic transmission, learning, and memory. Conclusion: The present results demonstrate that brain production of IL-6 regulates body weight in the context of caloric excess and that the cellular source is critical.

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PERFORMANCE OF LAYING HENS FED DIETS CONTAINING SOYBEAN GUMS, RAPESEED GUMS OR RAPESEED MEALS WITH AND WITHOUT GUMS

Canadian Journal of Animal Science ◽

10.4141/cjas81-127 ◽

1981 ◽

Vol 61 (4) ◽

pp. 1031-1040 ◽

Cited By ~ 2

Author(s):

H. W. HULAN ◽

F. G. PROUDFOOT

Keyword(s):

Body Weight ◽

Fatty Liver ◽

Egg Production ◽

Laying Hens ◽

Control Diet ◽

Egg Weight ◽

General Performance ◽

Mere Presence ◽

Per Se ◽

Sensitive Genotype

Two experiments were conducted to study the effect of soybean and/or rapeseed gums (SBG and/or RSG) on general performance and mortality of laying hens. In exp. 1 a total of 1824 SCWL hens of two commercial genotypes (912 of each) were fed a corn-soy-based control diet or this diet to which was added 2.0% be weight of either SBG or RSG. In exp. 2, 1824 hens of the more sensitive genotype used in exp. 1 were fed a control diet or diets containing approximately 15% of gummed and gumless Tower and Candle rapeseed mean (RSM) or gumless Tower RSM to which was added 3.33% Candle gums. The addition of SBG and RS3 to the diet (exp. 1) caused a significant increase in mortality, increased the amount of feed required to produce a kilogram of eggs, and decreased heart and liver weights. Feeding RSM with and without the commercial level (2.0% of the RSM, or in this study 0.3% of the diet) of added gums had no effect on body weight or egg weight. Hen-housed egg production and feed per dozen eggs were significantly depressed by feeding Candle RSM with gums and by feeding gumless Tower RSM to which had been added Candle gums, indicating that Candle gums but not Tower gums are deleterious to laying hens even at the commercial level of addition to RSM. Accumulated mortality to 497d was significantly higher for all groups fed RSM with or without gums compared to the control-fed group. The increase in mortality with this sensitive genotype appeared to be related to the mere presence of RSM in the diet and not due to the presence of gums per se. The major cause of mortality in both experiments was diagnosed as fatty liver syndrome, and from results of exp. 2 appeared to be related to the presence of RSM in the diet and not due to the presence of gums.

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STUDIES OF THE INSULIN-LIKE ACTIVITY OF SERUM

Acta Endocrinologica ◽

10.1530/acta.0.0570478 ◽

1968 ◽

Vol 57 (3) ◽

pp. 478-504 ◽

Cited By ~ 2

Author(s):

F. Gjedde

Keyword(s):

Adipose Tissue ◽

Serum Protein ◽

Serum Proteins ◽

Tap Water ◽

Protein Fractions ◽

Rat Diaphragm ◽

Crystalline Insulin ◽

Significant Difference ◽

Dialyzed Serum ◽

Insulin Like Activity

ABSTRACT The insulin-like activity (ILA) of serum and serum protein fractions was investigated by the adipose tissue and the rat diaphragm methods of bioassay. When undiluted serum was dialyzed against tap water at room temperature an increase in the ILA of the dialyzed serum was observed. The increase, which was localized in a precipitate of denatured globulins, corresponded per ml dialyzed serum to the effect of 100 000 μU crystalline insulin, measured by both methods. It was found that in the presence of calcium ions, a pH value between 7.7 and 8.1 and a temperature of about 37° C favoured the increase in ILA by dialysis. Treatment of serum protein fractions, separated in starch block electrophoresis, with dialysis by a standardized procedure against calcium containing water of low ionic concentration increased the ILA from about 4000 to 450 000 μU ILA per ml of fractionated serum. Of the ILA in the serum protein fractions, before and after dialysis, about two thirds was localized in the alpha globulins, and about one third in the beta-two globulins. A varying proportion of from 50 to 80 per cent of the ILA was suppressed by the addition of anti-insulin serum. Three serum proteins, found in other studies to bind radioactivity when incubated with labelled insulin, showed ILA. Following dialysis, a very marked increase in the ILA of all three globulins was observed. The ILA in the three serum proteins was found to account for most of the ILA in the serum. These findings were obtained by both the adipose tissue and the rat diaphragm bioassay, no significant difference being found between the results obtained by the two assays. Some hypotheses on the state of insulin in the blood are discussed in relation to these observations. It is concluded that the main part of ILA of serum is due to insulin associated with the three globulins, and that the activity of insulin is probably potentiated by binding to serum proteins.

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Response of growing rabbits to different levels of dietary citric acid

Bangladesh Journal of Animal Science ◽

10.3329/bjas.v39i1-2.9685 ◽

1970 ◽

Vol 39 (1-2) ◽

pp. 125-133

Author(s):

MR Debi ◽

KMS Islam ◽

MA Akbar ◽

B Ullha ◽

SK Das

Keyword(s):

Body Weight ◽

Citric Acid ◽

Immune Status ◽

Body Weight Gain ◽

Control Diet ◽

Feed Consumption ◽

Control Treatment ◽

Significant Difference ◽

Dietary Treatments ◽

Different Levels

An experiment was conducted for a period of 56 days with 36 healthy New Zealand White (NZW) rabbits aged about one months having weight from 370 to 390g to evaluate the effects of dietary citric acid on growth performance, feed consumption and digestibility of nutrients as well as immune status. The experiment was designed with 6 dietary treatments having 6 rabbits per treatment. Rabbits of control treatment (T1) were given the diet without citric acid (CA) but the dietary treatments T2, T3, T4, T5 and T6 contained 0.5, 1.0, 1.5, 2.0 and 2.5% CA respectively. Green grass was supplied on ad libitum basis. The total body weight gain was Addition of CA at the level of 2% enhanced body weight significantly (P<0.05). (P<0.05)(P<0.05) T 734, 776, 812, 862, 911 and 740g for the rabbits fed 0, 0.5, 1.0, 1.5, 2.0 and 2.5% CA containing diets respectively. Total DM intake also increased with increasing the percentage of CA up to 2% level . Incase of feed conversion ratio, there was no significant difference in addition to different levels of CA. Supplementation of CA improved dry matter, crude protein and ether extract digestibility but incase of crude fiber and nitrogen free extract, there was no significant difference. Non significant difference was also found incase of acidity of feed and feces. The highest lymphocyte (73%) was counted in dietary treatment T6 having 2.5% CA, which was 17% higher than the control diet. From the result of this experiment, it may be concluded that the addition of CA up to 2% level in diet improves performance, digestibility of nutrients and immune status of growing rabbit. Key words: Rabbit; Citric acid; Performance; Immunity DOI: http://dx.doi.org/10.3329/bjas.v39i1-2.9685 Bang. J. Anim. Sci. 2010, 39(1&2): 125-133

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Determination of Blood Serum Protein Fractions of Calves with Clinically Diagnosed Pneumonia

Indian Journal of Animal Research ◽

10.18805/ijar.b-1312 ◽

2020 ◽

Author(s):

Pınar Tanritanir Ekici ◽

Semiha Dede ◽

Veysel Yüksek ◽

Sedat Çetin ◽

Ayşe Usta

Keyword(s):

Serum Protein ◽

Patient Group ◽

Total Protein ◽

Protein Fractions ◽

Economic Losses ◽

Blood Parameter ◽

Serum Samples ◽

Protein Levels ◽

Significant Difference ◽

G Ratio

Background: Respiratory diseases in calves are causing worldwide economic losses for the beef industry. This study was planned to determine the serum protein fractions and A/G ratio in calves with clinically diagnosed pneumonia, and evaluate the possibility uses of these parameters as clinical diagnostic parameters.Methods: The 34 calves with respiratory system problems and 10 healthy calves without clinical pneumonia symptoms were used as materials. The obtained serum samples were used for total protein and serum protein electrophoresis.Result: Albumin, α1-globulin percentages and A/G ratio decreased in the patient group (p≤0.01), other globulin fractions were higher in the patient group (p≤0.01), for % g values. According to concentration results, it was found that while albumin did not show a significant difference, α1-globulin (p≤0.05) and A / G ratio (p≤0.01) decreased in the patient group. In addition, other globulin fractions and total protein levels were higher in patient groups (p≤0.01). As a result, the serum protein fractions should be evaluated as a useful biochemical blood parameter for diagnosis and follow up of lung diseases especially in calves.

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A comparison of soluble-protein fractions in two acanthocephalan genera

Canadian Journal of Zoology ◽

10.1139/z74-111 ◽

1974 ◽

Vol 52 (7) ◽

pp. 823-825 ◽

Cited By ~ 3

Author(s):

Eldon S. Eveleigh

Keyword(s):

Soluble Protein ◽

Relative Concentration ◽

Soluble Proteins ◽

Protein Fractions ◽

Electrophoretic Separation ◽

Starch Gel ◽

Two Stages

Micro-starch gel electrophoretic separation of soluble proteins from whole-Acanthocephala homogenates yielded band profiles useful for distinguishing between the genera Prosthorhynchus and Polymorphus. Certain protein bands were common to both genera while other bands were characteristic. Band patterns for two stages of Polymorphus were identical except for differences in the relative concentration of certain bands.

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Diversity and physical properties of rumen microbial ecosystem of West African dwarf rams fed ammonium sulphate-fortified diets

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i2.36 ◽

2020 ◽

Vol 46 (2) ◽

pp. 209-218

Author(s):

A. T Akinlade ◽

T. O. Ososanya

Keyword(s):

Body Weight ◽

Ammonium Sulphate ◽

Microbial Population ◽

Control Diet ◽

Intestinal Flora ◽

Basal Diet ◽

Feed Additive ◽

Microbial Ecosystem ◽

Rumen Microbes ◽

Significant Difference

Ammonium sulphate is a feed additive used in ruminant feeds mainly for stabilization of the intestinal flora, enhance the development of the adult rumen microflora, improve digestion and nitrogen flow towards lower digestive tract and improve meat and milk production. In the study the diversity of rumen microbial ecosystem of WAD rams fed ammonium sulphatefortified diets at 0, 2.5, 5.0 and 7.5g/kg to the concentrate diet as T1, T2, T3 and T4 respectively was assessed. Sixteen WAD rams with average body weight of 12.20+0.12 kg were used and fed for 109 days, each treatment had four replicates. In a completely randomized design and differences among treatment means were examined using least significant difference, each ram was fed at 5% of their body weight at 60:40 levels of experimental diet and wilted guinea grass as basal diet. Variables measured were: S. typhimurium, E. coli, bacteria, fungi and protozoa. The results indicated that establishment of the microbial population of diets consisting of 7.5g/kg (NH ) SO were significantly higher 4 2 4 in bacteria and fungi than the control diet (P<0.05). It was concluded that fortification a 7.5g/kg (NH ) SO give a better understanding of the role of rumen microbes and the 4 2 4 establishment of the microbial population, which help to maintain the host's health and improve animal performance.

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Physicochemical characterization of colored soluble protein fractions extracted from Spirulina (Spirulina platensis)

Food Science and Technology International ◽

10.1177/1082013218786862 ◽

2018 ◽

Vol 24 (8) ◽

pp. 651-663 ◽

Cited By ~ 3

Author(s):

Abakoura Barka ◽

Amal B Amira ◽

Frédéric Francis ◽

Christophe Blecker

Keyword(s):

Surface Tension ◽

Soluble Protein ◽

Physicochemical Characterization ◽

Protein Fractions ◽

Monolayer Films ◽

Collapse Pressure ◽

Pressure Surface ◽

Dynamic Methods ◽

Significant Difference ◽

Compression Isotherms

The aim of this study was to evaluate the physicochemical properties of Spirulina colored soluble protein fractions extracted from spray-dried Spirulina powder. Three fractions, including the blue soluble Spirulina protein, the green soluble Spirulina protein, and the total soluble Spirulina protein fractions were obtained. Investigations on their behavior at air/water interface were carried out using dynamic methods of drop volume and bubble pressure surface tension measurements. Evaluation of their monolayer films’ mechanical behavior was done via compression isotherms using Langmuir film balance. The protein contents of the fractions were 82.76, 82.29, and 74.53% for the blue, the green, and the total fractions, respectively. Surface tension decay increased with increasing concentration for all the fractions. No significant difference in surface tension decay was observed between the samples at 0.3% (w/w). Surface tension decay was less important at pH 3 for all the fractions. The total fraction and the blue fraction appeared to form more elastic films than the green fraction. The blue soluble fraction also presented the highest collapse pressure and initial expansion area.

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