Effects of plantago major extract on serum protein fractions in broiler diet

Author(s):  
Nuriye Tugba Bingol ◽  
Semiha Dede ◽  
Mehmet Akif Karsli ◽  
Orhan Yilmaz ◽  
Idris Turel ◽  
...  

The aim of this study was to determine the effects of Plantago major’s (P.major) water extract added into broiler diets at different levels on serum protein fractions. A total of 112 Ross 308 broiler chicks were used in the study. Experiment consisted of control and 3 treatment groups with a 28 chicks within each group. Each experimental group was divided into four subgroups consisting of 4 chicks. A basal (control) diet was prepared and three experimental diets were established by addition of P.major into basal diet; P.major 1 (5 g/kg feed), P.major 2 (10 g/kg feed), P.major 3 (15 g/kg feed). Broiler chicks were fed with these diets for 42 days ad libitum. Total protein levels of P.major 3 group were lower than other groups and controls. It was determined that the albumin percentages and levels became decreased in a significant ratio in the P.major 1 and P.major 2, (P<0,05), Alpha 1 globulin percentage and concentration were found significantly high in P.major 2 group than the other groups (P<0.05), Alpha 2 level and percentage in P.major 1 group was seen significantly higher than the control group, the A/G ratio in P.major 1 and P.major 2 was seen significantly lower than the control group. There was no significant difference between groups for beta and gamma globulins as percentage.

2012 ◽  
Vol 36 (1) ◽  
pp. 51-57
Author(s):  
Raghdad A. Abd Al-Jaleel

This study was conducted at the poultry farm ,Veterinary Public Health .,College of Veterinary Medicine, to study the effect of Tumeric (Curcuma longa) on broiler performance and some physiological traits .Two hundred fifty day-old (Rose308) broiler chicks were all located randomly to five treatments from 1-42 days of age, with tow replicate pens (25 birds /pen) per treatment .Chicks were fed the following :- Diet (1)Using basal diet free from herbal plants kept as control , Diet (2) Basal diet + 0.25% of Curcuma longa (250 gm/100kg of feed) Diet (3) Basal diet + 0.50% of Curcuma longa (500 gm/100kg of feed ).Diet (4) Basal diet + 1% 0f Curcuma longa (1000 gm/100kg of feed ).Diet (5) Basal diet plus 1.5 % of Curcuma longa (1500 gm/100kg of feed ).Results revealed that the inclusion of turmeric at the levels of 0.50% in the diets improved body weight ,feed conversion ratio ,there were a significant difference in feed consumption . At the same time there was no significant difference for edible parts, were as found significant difference (P˂ 0.05) for dressing percent for all treatments treat with compare for control group . At the same time there was no significant difference in PCV, RBC, Hb, WBC while there was significant difference in H/L ratio, Albumin and globulin.


Author(s):  
Pınar Tanritanir Ekici ◽  
Semiha Dede ◽  
Veysel Yüksek ◽  
Sedat Çetin ◽  
Ayşe Usta

Background: Respiratory diseases in calves are causing worldwide economic losses for the beef industry. This study was planned to determine the serum protein fractions and A/G ratio in calves with clinically diagnosed pneumonia, and evaluate the possibility uses of these parameters as clinical diagnostic parameters.Methods: The 34 calves with respiratory system problems and 10 healthy calves without clinical pneumonia symptoms were used as materials. The obtained serum samples were used for total protein and serum protein electrophoresis.Result: Albumin, α1-globulin percentages and A/G ratio decreased in the patient group (p≤0.01), other globulin fractions were higher in the patient group (p≤0.01), for % g values. According to concentration results, it was found that while albumin did not show a significant difference, α1-globulin (p≤0.05) and A / G ratio (p≤0.01) decreased in the patient group. In addition, other globulin fractions and total protein levels were higher in patient groups (p≤0.01). As a result, the serum protein fractions should be evaluated as a useful biochemical blood parameter for diagnosis and follow up of lung diseases especially in calves.


2021 ◽  
Vol 15 (2) ◽  
pp. 109
Author(s):  
Vera Iriani Abdullah

Linear growth retardation in childhood has decreased over the past few decades, but has not had a positive effect. In Indonesia, the change in the prevalence of stunting is around 37%. The nutritional status of pregnant women plays an important role in the incidence of stunting, which will be seen at the age of <5 years in 1000 days of their life. Pregnant women with poor nutrition and significant infection can give birth to a baby with stunting. For this reason, nutritional intake is needed, one of the animal foods that are high in protein is rebon shrimp about 62.4 g / 100 grams of dried shrimp. Protein has an important component so it is necessary for the physical development of children. The purpose of this study was to analyze serum protein levels before and after consuming rebon shrimp based PMT powder in the intervention group and control group and to analyze differences in serum protein levels for pregnant women before and after consuming PMT Koya powder made from rebon shrimp between the control and intervention groups. . This type of research is a quantitative study with a quasi-experimental method using the pre post test control group design approach. The number of respondents was 30 people who were divided into 2 groups, namely the intervention group and the control group. The intervention group was given koya powder as much as 100 grams / day for 7 days, assuming the fulfillment of 70 KKal. Data analysis using Data analysis using the Mann-Whitney test, if the data is not normally distributed. If the data are not normally distributed using the free sample t2 test. The conclusion of the analysis results to answer the research hypothesis. The results of the study were significant values P 0.000 <0.05. Conclusion There was a significant difference in serum protein levels of pregnant women before and after consuming PMT Koya powder made from boiled shrimp between the control and intervention groups.


2016 ◽  
Vol 3 (2) ◽  
pp. 323-329
Author(s):  
Masuma Habib ◽  
Abu Jafur Md Ferdaus ◽  
Md Touhidul Islam ◽  
Begum Mansura Hassin ◽  
Md Shawkat Ali

The study was conducted to investigate whether the productive performances and meat yield characteristics of broiler would be improved by feeding diet containing koroch (Pongamia Pinnata) seed cake (KSC) treated with NaOH and HCl. A total number of 160 day-old straight run broiler chicks were fed on 4 iso-energetic and iso-nitrogenous diets containing either basal diet with no KSC or basal diet containing 2% KSC treated with or without 1% NaOH or 1% HCl for a period of 28 days. These diets were considered as 4 different treatments. Each treatment was replicated 4 times, each having 10 birds. Inclusion of 2% KSC treated with NaOH or HCl in the diet resulted in lower live weight, live weight gain, feed intake, feed efficiency of broilers at all ages compared to inclusion of dietary 2% KSC alone (P<0.01). However, broilers, irrespective of age, fed on diet containing 2% KSC treated with 1% HCl exhibited better productive performances than those fed on 1% NaOH treated 2% KSC incorporated diet. The amount of feed intake of broilers on either KSC alone or HCl treated KSC was almost similar to that on control diet. The meat yield parameters showed a non-significant treatment effect except for the percentage of dressing yield, liver, gizzard, head and skin weight. Dressing percentage of broilers fed on HCl treated KSC at 2% level was significantly (P<0.05) lower compared to any other dietary treatment, whereas broilers in 2% KSC dietary group showed similar results to the control group. Percentage of head and skin weight relative to body weight were higher in treated (NaOH or HCl) KSC dietary group compared to either KSC alone or control group. However, the highest liver and gizzard weights were obtained from the broilers fed on diet containing 2% KSC treated with 1% HCl, followed by diet with 1% NaOH treated KSC, KSC alone diet and the control diet. The results of the present study clearly indicated that neither the 1% NaOH treated nor 1% HCl treated KSC alliviated the depressed productive performances and meat yield characteristics of broilers. It is concluded that the poor productive performances of broilers fed on KSC incorporated diet cannot be improved by treating KSC with either NaOH or HCl, and the oil extracted KSC may contain leftover oil which might have a potential toxic effect on growth and meat yield of broilers.Res. Agric., Livest. Fish.3(2): 323-329, August 2016


1991 ◽  
Vol 24 (4) ◽  
pp. 235-243 ◽  
Author(s):  
Eridan M. Coutinho ◽  
Frederico G. C. Abath ◽  
Lucila P. C. G. de Freitas ◽  
Aureni C. Salzano ◽  
Maria A. Lapa ◽  
...  

Body, liver and spleen weights; histopathology of the liver, spleen and intestines; hepatic and serum soluble proteins changes were the parameters studied in undernourished Swiss albino mice experimentally infected with S. mansoni. Non-infected deficient animab had lower liver/body weight and spleen/body weight ratios as compared to the controls (22.60% casein group). Infected mice showed higher values regardless the type of diet. Undernourished infected subgroup showed a persistent exudative periovular reaction in the liver. Soluble hepatic proteins content and serum protein fractions appeared to be lower in the deficient infected mice. A significant difference was detected in the gammaglobulin fraction between infected and non-infected animals fed the control diet with higher values for the former. Our data suggest that the effects of malnutrition, per se, are sometimes more detrimental to the host than those due to Manson 's schistosomiasis.


2006 ◽  
Vol 76 (6) ◽  
pp. 353-358 ◽  
Author(s):  
Wang ◽  
Zhu ◽  
Guo ◽  
Zhang ◽  
Jia

This experiment was conducted to investigate the influence of different dietary levels of zinc (Zn) on performance, vitamin B12, and blood parameters in lambs. Thirty six cannulated Poll Dorset × Small-tailed Han wether lambs were assigned randomly to four treatment groups: The control group, which was supplemented with 0.30 mg Co/kg dry matter (DM) to the basal diet; and the low-, medium- and high-Zn supplemented groups, supplementation of 50, 100, and 150 mg Zn/kg DM to the control diet, respectively. Lambs were housed in individual pens and the experiment lasted for 70 days. There was no significant difference in body weight gain and feed/gain between different treatment groups. The high-Zn supplemented lambs showed lower vitamin B12 concentrations in both ruminal fluid and plasma, and higher methylmalonic acid and homocysteine concentrations in plasma compared with the control and low-Zn supplemented groups (p < 0.05). No significant difference was observed in these biochemical values between the control, the low-, and the medium-Zn supplemented groups (p > 0.05). Plasma folate concentration, glucose, and heme-dependent blood parameters were not influenced by dietary zinc level. It was concluded that the higher level of zinc supplementation had a negative effect on vitamin B12 in lambs. Higher inclusion level of dietary zinc could inhibit vitamin B12 synthesis in the rumen of lambs.


Animals ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 117 ◽  
Author(s):  
Mehdi Qorbanpour ◽  
Taha Fahim ◽  
Faramin Javandel ◽  
Mehran Nosrati ◽  
Erwin Paz ◽  
...  

A total of 225 day-old male broiler chicks (Ross-308) were randomly allocated to five treatment groups, with three replicates in a completely randomized design for 42 days. Birds were fed a basal-diet supplemented with: no additive (control-diet), multi-strain probiotic (Lactobacillus acidophilus, Lactobacillus casei, Enterococcus faecium and Bifidobacterium thermophilum), or 0.15, 0.20 and 0.25% ginger (Z. officinale) powder, respectively. The results show no significant differences among treatments for growth traits and carcass characteristics, whereas using probiotics and ginger at all levels resulted in a significant decrease of gizzard weight and abdominal fat compared to the control group. Dietary treatments did not affect blood biochemistry and antibody production against sheep red blood cells (SRBC), IgG and IgM; however, antibody titre was higher in birds fed 0.25% ginger than other diets after 7 days post injection. The Lactobacillus counts in ileal content of birds fed 0.20 and 0.25% ginger were higher compared to the other treatments. In conclusion, dietary supplementation with either ginger or probiotics showed a significant influence on birds’ immune response, probably because ginger had strong antioxidant activity and the probiotics stimulated the production of natural antibodies.


2021 ◽  
Vol 2 (Supplement_1) ◽  
pp. A41-A41
Author(s):  
F Karuga ◽  
S Turkiewicz ◽  
M Ditmer ◽  
M Sochal ◽  
P Białasiewicz ◽  
...  

Abstract Circadian clocks are endogenous coordinators of 24-hour behavioral and molecular rhythms, which disruption may be caused by obstructive sleep apnea (OSA). It is composed of a set of genes, function as activators (CLOCK, BMAL) or repressors (PER, CRY). Neuronal PAS Domain Protein 2 (NPAS2) can substitute CLOCK in its function. Orphan nuclear receptor (Rev-Erb-α) is another protein supporting the CLOCK-BMAL1 complex, forming the loop which helps to regulate their expression. There are studies suggesting the significant influence of circadian disruption mediated via NPAS2 and Rev-Erb-α on DM2 development. The aim of the study was to determine the role of NPAS2 and Rev-Erb-α in DM2 for OSA patients. All participants underwent polysomnography (PSG) examination. Based on apnea-hypopnea index accompanied by clinical data the recruited individuals (n=40) were assigned to one from 3 groups: OSA (severe OSA, no DM2; n=17), DM2 (severe OSA + DM2; n=7) and control group (no OSA, no DM2; n=16). Serum protein levels of Rev-Erb-α and NPAS2 were assessed with ELISA immunoassay. Analysis between the groups revealed the statistically significant difference only in NPAS2 protein level (p=0.037). Further post-hoc analysis revealed significant differences between OSA and the control group (p=0.017). Moreover, a statistically significant correlation between AHI and NPAS2 serum protein level was observed (r=-0.478, p=0.002). NPAS2 protein levels are associated with a number of apneas and hypopneas during the REM phase of sleep and might have a significant role in the development of OSA complications. However, further studies are needed to understand its role.


Author(s):  
Nesrullah Aysin ◽  
Handan Mert ◽  
Nihat Mert ◽  
Kivanc Irak

Fucoidan is a sulfate polysaccharide extracted from brown algae. Fucoidan has various pharmacological properties, such as anti-tumor, anti-mutagenic, anti-inflammatory, antiviral, antioxidant, anti-fibrogenic activity and anti-complementary activities Objective: This study was aimed to explore the effect of fucoidan on biochemical parameters (ALT, AST, GGT, total protein, albumin, globulin) and protein fractions in hepatotoxicity induced by CCl4 in rats. Materials and Methods: The rats used in the study were randomly divided into four groups of 8 rats each: Control group, fucoidan group, fucoidan+CCl4 group and CCl4 group. After 24 hours from the process of an eight-day experiment, blood samples were taken. The analysis of ALT, AST, GGT activities and total protein, albumin, globulin levels were done by an autoanalyser and serum protein fractions (albumin, á1- globulin, á2-globulin â- globulin, ã- globulin and A/G ratio) were electrophoretically determined. Results: In the group of fucoidan+CCl4, it was determined that the levels of AST (p>0.001), GGT (p>0.001), total protein (p>0.01), globulin (p>0.01), â-globulin % (p>0.01), ã-globulin % (p>0.001) statistically decreased compared to CCl4 group. Conclusion: It can be said that fucoidan has the property of hepatoprotectant by looking to some biochemical parameters and changes in protein fractions that examined in hepatotoxicity induced by CCl4.


2019 ◽  
Vol 36 (01) ◽  
pp. 046-050
Author(s):  
Alireza Taghdisi ◽  
Sajjad Hejazi

Introduction Increasing the immune system's function of fighting infectious diseases is very important in the poultry industry. Ginger, scientifically known as Zingiber officinale, belongs to the Zingiberaceae family. The use of ginger in the diet of poultry increases serum levels of superoxide dismutase enzymes and glutathione peroxidase, which are considered to be important antioxidant enzymes. The main objective of the present study is to evaluate the effect of ginger on the spleen tissue of broiler chickens. Material and Methods The specimens comprised 2 groups of 20 Ross breed broiler chicks, for 42 days and were then, examined and tested. The diet was supplemented with 1 g/kg of ginger powder from the beginning of the rearing period. Blood samples of the chicks were randomly collected to measure the levels of hemagglutination (HI). The removed spleens were fixed with 10% formalin buffer. The specimens were cut in 5-micron diameters and stained with hematoxylin and eosin. Results and Conclusion There was a statistically significant difference in the mean of HI blood titers between the chicks in the growth period and final period groups (p < 0.05). The white-pulp tissue samples were more clearly seen in the treatment group than in the control group, and also, it was observed that the wall of the central artery of the white pulp was thicker in the ginger-treated group as compared with the control group. The nutritional value of ginger may vary. Thus, it is necessary to investigate the effect of this plant final on weight gain; the serum factors associated with the metabolic chart, and the response of the immune system to this plant.


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