scholarly journals Efektivitas Vaksinasi Crude dan Soluble Protein Spora Myxobolus Koi terhadap Tingkat Kerusakan Usus Ikan Koi (Cyprinus carpio Koi) [ The Effectivety Crude and Soluble Protein of Myxobolus Koi Spore againts Intestine Different Degrees in Koi (Cyprinus carpio Koi)]

2019 ◽  
Vol 7 (1) ◽  
pp. 55
Author(s):  
Rachma Woro Anggarani ◽  
Gunanti Mahasri ◽  
Lucia Tri Suwanti

Abstract Myxobolus koi is one species of Myxobolus sp that can cause parasitic diseases in fish called Myxobolusis. Based on the Decree of the Minister of Marine and Fisheries No: KEP.03/MEN/2010 that Myxobolus koi in the list of Fish Quarantine Pests group I. Myxosporean diseases are most numerous in the water can cause Proliferative Kidney Disease (PKD) and Whirling Disease (WD). The aim of this research is to finding, analyzing and determining the protein of spores Myxobolus koi that can effectively reduce the level of damage to the intestinal organs as well as for the prevention myxobolusis on Koi’s. Then for finding, analyzing and determining the protein of spores Myxobolus koi do isolation of spore proteins. The study consisted of three phases examination to preparation and identification of spores, isolation and analyze of crude and soluble protein spores for obtain dose and molecular weight each protein and histopathological test. This research uses descriptive method. The data presented may be narratives, images, tables or charts for each group. Intestinal histopathology test results of research carried scoring Koi’s were analyzed using the Kruskal-Wallis. The results showed a profile crude protein and soluble proteins from spores Myxobolus koi showed that the molecular weight of crude protein Myxobolus koi in this study was 150 kDa and 72 kDa and for soluble protein was 73 kDa. Results scoring the degree of infection caused by exposure to Myxobolus koi then statistically processed with an average yield of scoring in a sequence of 0; 1.6; 0.64 and 0.32. Statistical analysis showed no significant difference in the treatment of K + with crude protein, and K + with soluble proteins. Statistical analysis showed that there were significant differences in treatment with K+ and K-, K- with soluble protein and crude protein and soluble protein. Histopathological changes in the intestine in the form of inflammatory cell infiltration, necrosis and haemorage

2009 ◽  
Vol 66 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Drenka Turjacanin-Pantelic ◽  
Dragana Bojovic-Jovic ◽  
Biljana Arsic ◽  
Eliana Garalejic

Background/Aim. A modern approach to surgical treatment of tuboperitoneal infertility is based on laporascopic techniques. The aim of this study was to compare results of tuboperitoneal infertility treatment by the use of laparoscopy and classical laparotomy. Methods. A retrospectiveprospective study on 66 women treated operatively form tuboperitoneal infertility was performed. Data from patient's anamnesis and those related to the surgical treatment results, obtained by the use of an inquiry, were used in retrospective and prospective analysis, respectively. Chi-square test was used in statistical analysis. P value < 0.05 was considered significant. Results. Classical laparotomy was used on 34 women in a period from 1996 to 1997, while 32 women were operated laparoscopically in a period from 1999 to 2000. The results were as follows: a total number of conceived women was 16 (24%), seven in the group I (20.6%) and nine in the group II (28.1%); 13 women were with one pregnancy, six in the group I (17.6%) and seven in the group II (22%). Twice pregnant were three women, one in the group I (2.9%) and two in the group II (6.2%). The resulting pregnancies were: five women with abortion spontaneous, two in the group I (5.9%) and three in the group II (9.4%); two women with extrauterine pregnancy in the group I (5.9%); three with pretemporal birth, one in the group I (2.9%) and two in the group II (6.2%), while six women were with the temporal birth, two in the group I (5.9%) and four in the group II (12.5%). Statistical analysis showed that there was no significant difference in the results between these two groups. Conclusion. Surgical treatment of tubeperitoneal infertility, regardless of the used methods (classical laparotomy or laparoscopy) was successful in a great number of women. These methods have a great advantage over in vitro fertilization, and they should not be ignored.


2020 ◽  
Vol 24 (3) ◽  
pp. 73-81
Author(s):  
Janusz Nowak ◽  
Wojciech Przystupa ◽  
Anna Krawczuk

AbstractThe article presents results of research on the bulk and shaken density of two commercial fertilizers: Pulgran urea and universal nitrogen fertilizer Salmag. A statistical analysis of the obtained results of average density at the assumed level of significance proved a significant difference between shaken and bulk density investigated with the use of Engelsmann apparatus and shaken density tested with a laboratory shaker for both investigated fertilizers. The obtained test results and uncertainty of the measured values served for calculation of uncertainty of the standard complex bulk and shaken density determined in case of many uncertainties. Further, an analysis of the impact of error sources on the value of complex uncertainty was conducted. The final results of the measurement were presented according to the convention of the Central Office of Measures.


2019 ◽  
Vol 13 (1) ◽  
pp. 31-35
Author(s):  
Zahra Jamali ◽  
Mahdieh Alipour ◽  
Syamand Ebrahimi ◽  
Marzie Aghazadeh

Background. Halitosis (oral malodor) is a common problem all over the world and its prevalence has been estimated at 23‒ 50%. Halitosis originates from oral cavity in 85% of patients. This clinical trial was conducted to evaluate the efficacy of the Halita mouthwash in oral halitosis treatment. Methods. Fifty subjects with an organoleptic score of >2 at baseline participated in this triple-blinded clinical trial. Subjects were divided into 2 groups. Group I subjects (N=25) were instructed to rinse with 0.2% chlorhexidine (CHX) mouthwash twice a day for 1 week. Group II subjects (N=25) used Halita mouthwash with the same instruction. Halitosis was evaluated at baseline and one week after using the mouthwashes by organoleptic method. Data were analyzed with chi-squared and Mann-Whitney U tests (P<0.05). Results. In the Halita group subjects exhibited 2.04±0.65 reduction in OLS. OLS reduction in the chlorhexidine group was 1.95±0.74. Statistical analysis showed no significant difference between the two groups (P>0.05). Conclusion. Based on the results, Halita mouthwash has the same effect on oral halitosis as routine 0.2% CHX mouthwash. Halita mouthwash has fewer side effects because of lower concentration of chlorhexidine. Therefore 0.2% CHX mouthwash could be replaced by Halita mouthwash for the treatment of halitosis.


Author(s):  
Ozgun Iyigun ◽  
Tugce Uzar ◽  
Irem Karaman ◽  
Adem Dirican ◽  
Sevket Ozkaya

Objective: The bronchodilator test is a useful method for measuring the changes in lung capacity with spirometry after inhaling a short-acting bronchodilator drug to diagnose patients with obstructive lung disease. Although its liquid form was available, the inhaler form of levosalbutamol is a relatively new short-acting bronchodilator. To measure the efficacy of levosalbutamol in acute bronchodilator reversibility, in this study, we aimed to compare the effects of salbutamol and new levosalbutamol/ipratropium combination on early reversibility and FEV1 changes observed in bronchodilator tests. Methods: Bronchodilator test results of forty-two patients who were selected according to the inclusion criteria were retrospectively analyzed. The results of spirometry analysis were evaluated for twenty-four patients who received salbutamol (Group I) and eighteen patients who received levosalbutamol/ipratropium combination (Group II). Reversibility levels were evaluated as the absolute and percentage changes in FEV1. Results: The changes of absolute and percentage values of mean FEV1 were 159±118 mL, 12.2%±11.4% in salbutamol (Group I) group and 233±191mL, 18.8%±15.6% in levosalbutamol / ipratropium (Group II) combination (p=0.025 and 0.048, respectively). Although no significant difference was observed in the reversibility results, Group II showed significantly improved FEV1 values compared to Group I. Conclusion: To the authors’ knowledge, this is the first study demonstrating that the combination of inhalation therapy with levosalbutamol and ipratropium bromide is more beneficial than salbutamol alone in terms of FEV1 improvement in bronchodilator test. We suggest that this combination can be a good candidate for utilization in diagnostic bronchodilator tests since it proved a specific higher improvement in FEV1 values.


2016 ◽  
Vol 11 (3) ◽  
Author(s):  
Ashraf Zia ◽  
Tahir Chaudhry ◽  
Riaz Hussain ◽  
Tafoorul Islam Ghalani

In this study total hundred patients wee included. Fifty (group I) were subjected to COPA device for airway maintenance and in other fifty (group II) LMA was used. In this study first attempt successful insertion rate in group I was 90% while in group II it was 94%. However statistical analysis showed no significant different in both groups. Regarding maneuvers needed to maintain smooth breathing in group I head tilt was used in 2 %. It is significantly high in group I than in group II. Laboured breathing was seen in 6% in group I while 2% in group II. There was no significant difference in both groups. According to this study LMA is better in all respect as it is easier to insert and better fit in.


2016 ◽  
Vol 04 (02) ◽  
pp. 117-121
Author(s):  
Leena Verma ◽  
Jyoti Gupta ◽  
Sidhi Passi

Abstract Aim: This study was designed to assessthe prevalence of various oral habits among 3-6 years old school childrenin Chandigarh Method: Fifteen different public schools were randomly selected among 45 public schools in Chandigarh area from where 938 children (3-6 yr) were randomly selected (511 boys and 427 girls) with the objectives of recording the prevalence of oral habits. Statistical analysis was carried out using SPSS 10.0 software and the statistical significance of oral habits as related to age groups and sex were calculated by using ANOVA test. Results: The overall prevalence of oral habits in children was 11.7%. Nail biting was the commonest habit (5.5%) followed by thumb sucking (4.05%), tongue thrusting (1.8%), mouth breathing(0.3%) and lip biting(0%). Also, there were no significant differences between boys and girls with respect to prevalence of oral habits. However, a significant difference was observed between different age groups with respect to the prevalence of oral habits. Conclusions: The data revealed that a significant percentage of the children had deleterious oral habits which can contribute towards the development of malocclusion. This helps in identifying children who are in need to intercept the oral habits at earliest to avoid future occurrence of malocclusion.


DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 60
Author(s):  
Sularsih Sularsih ◽  
Michelle Suhartono ◽  
Nafi’ah Nafi’ah

<p><strong><em>Background:</em></strong><em> Traumatic ulcer is one of the most common oral wounds. Chitosan has mucoadhesive characteristic while Aloe vera containing lignin which is able to penetrate the skin. It is expected that the combined gel of chitosan and Aloe vera will function as wound healing accelerator in traumatic ulcer. Molecular weight is one of the characteristics of chitosan quality. <strong>Purpose:</strong> the aim of this experiment was to know the density of collagen fibers in wound healing of traumatic ulcer using the combined gel of chitosan with different molecular weight and Aloe vera. <strong>Materials and method: </strong>30 Male Rattus Norvegicus were divided into 3 groups. Group I was control group (without chitosan and Aloe vera), group II was given low molecular weight chitosan and Aloe vera, group III was given high molecular weight chitosan and Aloe vera. The groups were given traumatic ulcer making with 4 mm diameter and 2 mm depth. Rats were sacrificed by decapitation on day 3 and 7 then they were examined histopatologically to see the density of collagen fibers. <strong>Result:</strong> Statistical analysis with Kruskall Wallis and Mann-Whitney U test showed that there were significant difference p&lt;0,05 between high and low molecular weight chitosan with Aloe vera group on day 3 and 7. <strong>Conclusion:</strong> Chitosan with high molecular weight and Aloe vera were more effective in wound healing of traumatic ulcer b</em><em>ecause they increase</em><em> the density of collagen fibers.</em><em></em></p><p><strong><em> </em></strong></p><p><strong><em>Keywords:</em></strong><em>  Combined gel of chitosan and Aloe vera, density of collagen fibers, wound healing.</em><em></em></p><p><strong><em> </em></strong></p><strong><em>Korespondensi:</em></strong><em> Sularsih, Bagian Ilmu Biomaterial Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Hang Tuah, Arif Rahman Hakim 150, Telepon 031-5912191.</em>


2016 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Eka Fitrianda ◽  
Wida Ningsih

Eupatorium odoratum L. is traditionally used to treat open wounds. Aim of this study was toinvestigate the healing activity of ointment containing extract of E. odoratum in burns induced in malealbino mice. Mice were divided into 5 groups, all were induced for burn wound using a heat stamp intemperature 80°C for 20 minutes. Group I was treated with ointment base (control), group II, III and IVwere treated with extract ointment in concentration of 5%, 10%, and 20% w/w respectively, and the lastgroup V was treated with ointment reference. Observations were made during 21 days exactly on the 7th,14th and 21st day, which included parameters: percentage of healed area, epithelialization time andcollagen scores. The result showed that on 14th day, mean of healed area in group III (75.89%±7.76%)and IV (76.29%±6.981%) were significantly higher than other groups (P<0.05). On the 14th and 21th day,collagen scores in groups III (2.33±0.577 and 2.67±0.577) and IV (2.67±0.577 and 2.33±0.577) are equalwith reference group, these score are significantly higher than in groups I and II (P<0.05). Meanwhile,though epithelialization time in group III and IV (both 14.33 days±0.577days) are more quickly thanothers, statistical analysis showed that there is no significant difference on time between all groups(P>0.05).


1991 ◽  
Vol 24 (4) ◽  
pp. 235-243 ◽  
Author(s):  
Eridan M. Coutinho ◽  
Frederico G. C. Abath ◽  
Lucila P. C. G. de Freitas ◽  
Aureni C. Salzano ◽  
Maria A. Lapa ◽  
...  

Body, liver and spleen weights; histopathology of the liver, spleen and intestines; hepatic and serum soluble proteins changes were the parameters studied in undernourished Swiss albino mice experimentally infected with S. mansoni. Non-infected deficient animab had lower liver/body weight and spleen/body weight ratios as compared to the controls (22.60% casein group). Infected mice showed higher values regardless the type of diet. Undernourished infected subgroup showed a persistent exudative periovular reaction in the liver. Soluble hepatic proteins content and serum protein fractions appeared to be lower in the deficient infected mice. A significant difference was detected in the gammaglobulin fraction between infected and non-infected animals fed the control diet with higher values for the former. Our data suggest that the effects of malnutrition, per se, are sometimes more detrimental to the host than those due to Manson 's schistosomiasis.


Author(s):  
Eman Sutrisna ◽  
Qurrotu Aini ◽  
Ika Murti Harini ◽  
Nur Signa Aini Gumilas ◽  
Mustofa Mustofa ◽  
...  

Phaleria macrocarpa (Scheff.) Boerl. has been widely used in the treatment of diabetes mellitus but its effectiveness is still inconclusive so it needs to be compared with standard drugs such as metformin. This study aims to analyze the effects of P. macrocarpa fruit extracts on blood sugar levels and the repairing of the pancreatic Langerhans islets of diabetic rat models. The study was conducted experimentally by pre and post-test design with a control group. Thirty white rats were divided into 6 groups. All rats were treated with 40 mg/200 g BW of streptozotocin (STZ) to induce hyperglycemia, except group I. Group II as a negative control was given with aquadest. Group III, IV, and V were given with ethanol extract of P. macrocarpa pericarps at doses of 200, 250, and 300 mg/200 g BW/day, respectively. Group VI was given with metformin at a dose of 150 mg/200 g BW/day. On the 22nd day of treatment, blood specimens were taken for examination of fasting blood sugar levels and pancreatic organs were analyzed for histopathological examination of the islets of pancreatic Langerhans. The data were statistically analyzed with α<0.05; CI95%. Paired sample t-test results showed a significant decrease in blood sugar levels before and after treatment in group V (p-value=0.048). One way anova and post hoc Least Significant Difference (LSD) test results showed a significant difference in mean blood sugar levels after the final treatment between groups I and II (p-value=0.013), I and III (p-value=0.004), I and IV (p-value=0.002), I and VI (p-value=0.000), II and VI (p-value=0.031), and V with VI (p-value=0.005); one way anova and post hoc LSD test results showed a significant difference in the mean area of islets of pancreatic Langerhans between group I and all treatment groups (p-value=0.000), II with V and VI (p-value=0.000 and 0.009), III with V and VI (p-value=0.000 and 0.001), and IV with V (p-value=0.003). It can be concluded that the extract of P. macrocarpa pericarps showed a hypoglycemic effect and repaired the damage in Langerhans islets of STZ-induced rat in a dose-dependent manner.


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