scholarly journals Research of Klebsiella pneumoniae in dairy herds

2015 ◽  
Vol 35 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Helio Langoni ◽  
Marcos Vinicius S. Guiduce ◽  
Diego B. Nóbrega ◽  
Rodrigo C. da Silva ◽  
Virgínia B. Richini-Pereira ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Rapid Evolution ◽  
Poor Response ◽  
Subclinical Mastitis ◽  
Dairy Herds ◽  
Rrna Gene ◽  
Toxic Shock ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Hind Limbs

Klebsiella pneumoniae is a common environmental agent of clinical and subclinical mastitis affecting dairy herds, and may be present in the final product decreasing its quality. Mastitis caused by K. pneumoniae is even more severe due to its poor response to antibiotic therapy, rapid evolution to toxic shock and death of the animal. This paper aimed to study the prevalence of this pathogen among dairy herds in ten farms located in different municipalities of São Paulo State based on size and use of milking technology. All mammary glands of all lactating cows were screened using the California Mastitis Test (CMT) and a strip cup. A single aseptic milk sample (20mL) was collected from all CMT-positive quarters and bulk tanks, whereas swab samples were collected from feces, hind limbs of the animals, bedding and milking parlor. Identification of K. pneumoniae was performed using conventional microbiology culture, biochemical assay and Polimerase Chain Reaction. The primers were designed and tested at the Laboratory of Molecular Biology applied to Zoonoses (FMVZ, Unesp-Botucatu) targeting the 16S rRNA gene. This study included 1067 animals. Six cases of intramammary infection by K. pneumoniae were detected in six different cows in two farms. Moreover, K. pneumoniae was isolated in 77 swabs (34 from bedding in 9 farms, 7 from waiting rooms in 5 farms, 6 from milking parlors in 4 farms, 11 from rectums in six farms, and 19 from hindlimbs in 7 farms. Molecular analysis confirmed the agent was K. pneumoniae. At least one strain of the agent was identified in a certain site in all farms, showing the need of maintaining the hygiene in dairy farms.

scholarly journals PREVALENCE OF SUBCLINICAL MASTITIS IN DAIRY COWS IN SELECTED AREAS OF BANGLADESH

2012 ◽  
Vol 9 (1) ◽  
pp. 73-78 ◽  
Author(s):  
MA Islam ◽  
MZ Islam ◽  
MA Islam ◽  
MS Rahman ◽  
MT Islam
Keyword(s):  
Dairy Cows ◽  
Rural Areas ◽  
Dairy Farm ◽  
Local Area ◽  
Subclinical Mastitis ◽  
Local Breed ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Late Stages

This research was carried out to determine the prevalence of subclinical mastitis in lactating Dairy Cow of Bangladesh Agricultural University dairy farm (BAUDF) and rural areas of Tangail sadar upazila of Bangladesh during the period of July 2009 to April 2010. A total of 200 milk samples (40 from BAUDF and 160 from Tangail sadar upazila) were collected for this study which were subjected to physical examination and subsequently screened for subclinical mastitis using three indirect tests viz. White Side Test (WST), California Mastitis Test (CMT), and Surf Field Mastitis Test (SFMT). Overall prevalence of subclinical mastitis (SCM) in lactating dairy cows found in this study was 29%. Cows were infected with SCM 29.5%, 27.5% and 25.5% detection by CMT, WST and SFMT respectively. Higher prevalence of SCM was detected in milch crossbred cows (36.36%) in comparison to local bred cows (24.61%) maintained under extensive management system in Rural area of Tangail sadar upazila. The prevalence of SCM was recorded in 31.58%, 30.76% and 68.75% in cows of local area of Tangail sadar upazila, and 25.0%, 40.0% and 71.42% in cows of BAU,DF during the early, mid and late stages of lactation respectively. The highest prevalence of SCM was recorded during the early lactation stage in both the local breed cows (30.0%) and cows of BAUDF (45.83%) in comparison to their respective mid and late stages of lactation. The prevalence of SCM was highest in lactating cows having third lactation and high yielding (cows produced >10 liter milk per day) both in local breed and crossbred cows.DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11216 Bangl. J. Vet. Med. (2011). 9(1): 73-78 

Using milk leukocyte differentials for diagnosis of subclinical bovine mastitis

2017 ◽  
Vol 84 (3) ◽  
pp. 309-317 ◽  
Author(s):  
Juliano Leonel Gonçalves ◽  
Roberta L. Lyman ◽  
Mitchell Hockett ◽  
Rudy Rodriguez ◽  
Marcos Veiga dos Santos ◽  
...  
Keyword(s):  
Bovine Mastitis ◽  
Dairy Herd ◽  
Inflammatory Cells ◽  
Subclinical Mastitis ◽  
Absolute Number ◽  
Dairy Herds ◽  
Milk Samples ◽  
Lactating Cows ◽  
Automated Instrument ◽  
Culture Positive

This research study aimed to evaluate the use of the milk leukocyte differential (MLD) to: (a) identify quarter milks that are culture-positive; and (b) characterize the milk leukocyte responses to specific groups of pathogens causing subclinical mastitis. The MLD measures the absolute number and relative percentage of inflammatory cells in milk samples. Using the MLD in two dairy herds (170 and 172 lactating cows, respectively), we studied all lactating cows with a most recent monthly Dairy Herd Improvement Association somatic cell count (SCC) >200 × 103 cells/ml. Quarter milk samples from 78 cows meeting study criteria were analysed by MLD and aseptically collected milk samples were subjected to microbiological culture (MC). Based upon automated instrument evaluation of the number and percentage of inflammatory cells in milk, samples were designated as either MLD-positive or – negative for subclinicial mastitis. Positive MC were obtained from 102/156 (65·4%) of MLD-positive milk samples, and 28/135 (20·7%) of MLD-negative milk samples were MC-positive. When MC was considered the gold standard for mastitis diagnosis, the calculated diagnostic Se of the MLD was 65·4% (IC95% = 57·4 to 72·8%) and the Sp was 79·3% (IC95% = 71·4 to 85·7%). Quarter milks positive on MC had higher absolute numbers of neutrophils, lymphocytes and macrophages, with higher neutrophils% and lymphocytes% but lower macrophages%. The Log10 (N/L) ratios were the most useful ratio to differentiate specific subclinical mastitis quarters from healthy quarters. Use of the MLD on cows with monthly composite SCC > 200 × 103 cells/ml for screening at quarter level identified quarters more likely to be culture-positive.In conclusion, the MLD can provide an analysis of mammary quarter status more detailed than provided by SCC alone; however, the MLD response to subclinical mastitis was not found useful to specifically identify the causative pathogen.

scholarly journals Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ma. Fabiola León-Galván ◽  
José E. Barboza-Corona ◽  
A. Arianna Lechuga-Arana ◽  
Mauricio Valencia-Posadas ◽  
Daniel D. Aguayo ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Bovine Mastitis ◽  
Dairy Herd ◽  
Subclinical Mastitis ◽  
Herd Size ◽  
Clinical Mastitis ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
California Mastitis Test ◽  
Streptococcus Uberis

Thirty-two farms (n=535cows) located in the state of Guanajuato, Mexico, were sampled. Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins ofBacillus thuringiensiswas tested. Forty-six milk samples were selected for their positive California Mastitis Test (CMT) (≥3) and any abnormality in the udder or milk. The frequency of SCM and CLM was 39.1% and 9.3%, respectively. Averages for test day milk yield (MY), lactation number (LN), herd size (HS), and number of days in milk (DM) were 20.6 kg, 2.8 lactations, 16.7 animals, and 164.1 days, respectively. MY was dependent on dairy herd (DH), LN, HS, and DMP<0.01, and correlations between udder quarters from the CMT were around 0.49P<0.01. Coagulase-negative staphylococci were mainly identified, as well asStaphylococcus aureus,Streptococcus uberis,Brevibacterium stationis,B. conglomeratum, andStaphylococcus agnetis. Bacterial isolates were resistant to penicillin, clindamycin, ampicillin, and cefotaxime. Bacteriocins synthesized byBacillus thuringiensisinhibited the growth of multiantibiotic resistance bacteria such asS. agnetis,S. equorum,Streptococcus uberis,Brevibacterium stationis, andBrachybacterium conglomeratum, but they were not active againstS. sciuri, a microorganism that showed an 84% resistance to antibiotics tested in this study.

Quarter-wise prevalence of subclinical mastitis in crossbred cows

2017 ◽  
Author(s):  
Rachna Ullah ◽  
Manju Ashutosh ◽  
Sujata Pandita ◽  
Ashutosh . ◽  
Panjab Singh Yadav ◽  
...  
Keyword(s):  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Research Needs ◽  
Crossbred Cows ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Karan Fries

The present study was designed to determine the quarter-wise and animal-wise prevalence of sub-clinical mastitis in crossbred Karan Fries (Holstein x Tharparker) lactating cows. For this purpose, the udders of 73 cows were screened by modified California Mastitis Test (mCMT) and EC measurements. The efficiency of detection of mastitis and non-mastitis by EC measurements was 86% and 93% respectively. Animal-wise prevalence was found 41%, while quarter-wise prevalence was 31.16%. Among mastitic quarters, prevalence of single quarter infection, two quarter infection, three quarter infection and all four quarter infection per animal, was 0.00, 26.66, 43.33 and 30.00%, respectively. Out of all quarters, maximum prevalence was found in right hind quarters (29.67%) followed by left hind (26.37%), left fore (23.07%) and right fore (20.87%).The same pattern was observed during EC measurements. Though EC showed similarity with CMT in the detection of subclinical mastitis, the research needs to be carried out on more number of animals.

scholarly journals Prevalence and Bacterial Isolates of Mastitis in Dairy Farms in Selected Districts of Eastern Harrarghe Zone, Eastern Ethiopia

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Tesfaheywet Zeryehun ◽  
Gerema Abera
Keyword(s):  
Dairy Cows ◽  
Bacterial Pathogens ◽  
Dairy Farms ◽  
Subclinical Mastitis ◽  
Coagulase Negative Staphylococcus ◽  
Eastern Ethiopia ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Associated Risk Factors ◽  
Standard Techniques

The study was conducted from November 2015 to April 2016 to estimate the prevalence of clinical and subclinical mastitis in lactating cows, to assess the associated risk factors, and to isolate the major bacterial pathogens in dairy farms in selected district of Eastern Harrarghe Zone, Eastern Ethiopia. The study was carried out in 384 dairy cows based on data collection, farm visit, animal examination, California mastitis test (CMT), and isolation bacterial pathogens using standard techniques. In the present study the overall mastitis at cow level was 247 (64.3%). The prevalence of clinical and subclinical mastitis and quarter level prevalence for clinical and subclinical mastitis were 12.5% and 51.8% at cow level and 10.7% and 46.4% at quarter level, respectively. Clinically, 101 (6.6%) quarters which belong to 75 (19.5%) animals were found to be with blind teat. In the present study prevalence of mastitis was significantly associated with parity and age (p<0.05). Bacteriological examination of milk sample revealed 187 isolates where coagulase negative Staphylococcus species (CNS) (34.2%) was the predominant species while Streptococcus faecalis (2.1%) was identified as the least bacteria. The present study concluded that prevalence of mastitis particularly the subclinical mastitis was major problem of dairy cows in the area and hence warrants serious attention.

scholarly journals Prevalence of Bovine Mastitis in the Western Region of Cameroon

2021 ◽  
pp. 1-5
Author(s):  
Müller FOTSAC DZOUSSE ◽  
◽  
Marc K. KOUAM ◽  
Keyword(s):  
Bovine Mastitis ◽  
Clinical Signs ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Feeding Method ◽  
Lactating Cows ◽  
Stage Of Lactation ◽  
California Mastitis Test ◽  
Farm Type ◽  
Age Range

Mastitis is the major disease in milking cows causing huge losses to the dairy industry worldwide. It is in view to contribute to the eradication of this pathology that the present study was carried out in cattle farms of the West Region of Cameroon from March 2020 to November 2020. A total of 473 lactating cows of local and improved breeds were screened for mastitis. Screening for clinical and sub-clinical mastitis was based on the observation of clinical signs and on the use of the California Mastitis Test (CMT) respectively. The overall prevalence was 34.88%, among which 9.72% and 25.16% represented clinical and subclinical mastitis respectively. In summary, the factors influencing the occurrence of mastitis were: the divisions surveyed (p<0.001), breed type (p=0.003), age range (p<0,001), farm type (p=0.05), soil type (p<0,001), cleaning frequency (p<0.001), feeding method (p<0.001), hygiene of udder (p=0.05) and stage of lactation (p=0.02). The results obtained allow us to recommend an improvement of the breeding conditions to prevent mastitis, because once mastitis appears in a farm, it becomes very difficult to eradicate it.

scholarly journals Prevalence of Subclinical Mastitis in Lactating Cows and Efficacy of Intramammary Infusion Therapy

2014 ◽  
Vol 12 (1) ◽  
pp. 55-61 ◽  
Author(s):  
TK Tripura ◽  
SC Sarker ◽  
SK Roy ◽  
MS Parvin ◽  
RR Sarker ◽  
...  
Keyword(s):  
Subclinical Mastitis ◽  
Cross Sectional Study ◽  
Infusion Therapy ◽  
Sectional Study ◽  
Cross Sectional ◽  
Milk Samples ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Infusion Tube ◽  
Smallholder Dairy Farms

The aim of the study was to estimate the prevalence of subclinical mastitis (SCM) in lactating cows of Mymensingh and Lakshmipur sadar upazilas and to evaluate the efficacy of intramammary infusion in subclinical mastitis affected cows. A cross-sectional study was carried out on 78 smallholder dairy farms using a pretested questionnaire to collect data from June 2011 to May 2012. Milk samples from 139 lactating cows were screened for SCM by California Mastitis Test. A total of 72 cows was found positive for SCM and treated with the Neomastipra-JR5® intramammary infusion tube (Hipra, Spain). The overall prevalence of subclinical mastitis in lactating cows was 51.8%, of which 51.4% at Mymensingh sadar and 52.2% at Lakshmipur sadar upazilas. The prevalence of subclinical mastitis was significantly increased with age and parity of cows. In left hind quarter, the prevalence of subclinical mastitis was significantly higher (33.1%) than those of other quarters. The efficacy of intramammary infusion with Neomastipra-JR5® in lactating cows was 66.7%.DOI: http://dx.doi.org/10.3329/bjvm.v12i1.20464 Bangl. J. Vet. Med. (2014). 12 (1): 55-61 

scholarly journals Subclinical intramammary infection does not affect bovine milk ethanol stability

2018 ◽  
Vol 55 (2) ◽  
pp. e135173
Author(s):  
Cristian Marlon de Magalhães Rodrigues Martins ◽  
Juliano Leonel Gonçalves ◽  
Bruna Gomes Alves ◽  
Marcos André Arcari ◽  
Marcos Veiga dos Santos
Keyword(s):  
Bovine Milk ◽  
Subclinical Mastitis ◽  
Milk Composition ◽  
Dairy Herds ◽  
Intramammary Infection ◽  
Milk Samples ◽  
Lactating Cows ◽  
Natural Exposure ◽  
Major Pathogens ◽  
Ethanol Stability

The present study hypothesized that intramammary infection (IMI) might reduce milk ethanol stability (MES), mainly when IMI is caused by major pathogens. Thus, this study evaluated the effect of IMI on bovine MES using a natural exposure experimental design. Ninety-four lactating cows from five dairy herds were selected once they were determined to have an IMI, based on milk bacteriological culturing with positive isolation and somatic cell count (SCC) > 200×103 cells/mL in two out of three composite milk samples collected during three consecutive weeks. After selection, cows were sampled a second time (within two weeks) for evaluation at mammary quarter level (n = 326): milk yield (kg/quarter/day), MES, composition (fat, protein, lactose, casein, total solids and solids-non-fat), and bacteriologic culture. The effect of subclinical mastitis on MES was tested by two models: 1) comparison of healthy vs. infected quarters; and 2) comparison of contralateral mammary quarter within cow. The only milk composition variable associated with MES was lactose (r = 0.18; P < 0.01). Subclinical IMI did not affect MES when the comparison was performed using both models (1 and 2). Likewise, MES did not change when infected quarters were sorted into two groups of pathogens (major, minor and infrequent; and contagious, environmental, minor and infrequent) and compared with healthy mammary quarters. Considering the results of both models, subclinical IMI did not affect MES of dairy cows.

scholarly journals Association of selected risk factors with bovine subclinical mastitis

2021 ◽  
Vol 15 (2) ◽  
pp. 153-160
Author(s):  
Nesma Helmy Youssif ◽  
Nagah Mostafa Hafiz ◽  
Mohamady Ahmed Halawa ◽  
Mena Fouad Saad
Keyword(s):  
Risk Factors ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Mean Value ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Holstein Friesian ◽  
Significant Difference ◽  
Parity Number

A total of 695 Holstein Friesian lactating dairy cows were examined for the prevalence of subclinical mastitis (SCM) and its association with selected risk factors in a private dairy farm at Fayoum district, Egypt. 444 lactating cows with 1145 quarters were found positive for SCM with prevalence of 63.88% and 41.18% at cow and quarter level respectively based on California Mastitis Test (CMT), Electrical Conductivity (EC) that had a mean value of 6.27 ± 0.066 mS/cm and Somatic Cell Count (SCC) with a mean value of 8.8×105± 9.2×103 cells/ml. The risk factors investigated in this study revealed that the highest percentage of SCM was observed in winter, in cows at the late lactation stage with a percentage of 32.21 and 59.91 respectively. The results concluded that the young age cows were exposed to SCM more than old ones. While the occurrence of SCM was higher in cows with 2nd, 3rd and 1st than those with 4, 5, 6th parity number. The analytical results also revealed that there is a highly significant difference (p < 0.05) between numbers of parity. However, there is no significant difference (p > 0.05) between different stages of age, lactation and season as risk factors.

scholarly journals The effect of subclinical mastitis on qualitative composition of milk

2021 ◽  
Author(s):  
A.Yu. Aliev ◽  
S.V. Fedotov ◽  
N.S. Belozertseva ◽  
I.M. Yakhaev
Keyword(s):  
Dry Matter ◽  
Whey Proteins ◽  
Titratable Acidity ◽  
Subclinical Mastitis ◽  
Matter Content ◽  
Excretory System ◽  
Dry Matter Content ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Experimental Group

A complex of mammological studies was carried out in experimental farms of the Vladimir region. Lactating cows underwent an express diagnosis of the health of the udder with the California mastitis test, and based on the result obtained, three groups of animals were formed, 20 animals each. The animals of the first group had a questionable reaction to the California express method; animals the second - weakly positive; the third group included blood with negative reaction to tests (control). In studies of milk from cows with suspected udder inflammation, a decrease in dry matter content and a change in the quantitative ratio between the constituents of milk were found. The dry matter content in the milk of the cows of the 1st experimental group exceeded this indicator in the milk of the 2nd group of cows by 7.6%; and lagged behind the control by 12.8%. When milk from sick cows was heated, some of the whey proteins were destabilized. The titratable acidity of the milk of the cows of the 1st experimental group was within the limits stipulated by GOST for the prepared milk, while in the cows of the 2nd group it was reduced to 13.22 ± 0.16 ° T, which indicates the onset of the inflammatory process in the mammary gland. When carrying out echographic visualization of the excretory system of the udder of cows, the following features were revealed: with a large volume of the milk cistern, the main type of branching of the excretory system was observed; with medium and small - mixed type; and with mild - loose As a result of the experiment, it can be concluded that animals with a weakly positive reaction to the California Mastitis Test are less productive and they receive milk of lower quality, which is practically not suitable for further use for technological purposes. However, according to the current Technical Regulations, this milk can correspond to the first or second grade.

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