Induction of mutations for earliness in the soybean cultivar Paraná

Mutation induction breeding through gamma ray seed treatment (22 krad) was used to obtain earliness in the soybean cultivar Paraná. Plants flowering earlier than Paraná were selected at the M3 generation. Eight mutant lines were isolated by further selections through several generations and tested in four yield trials carried out in 85/86 (Piracicaba) and 86/87 (Piracicaba, Sertãozinho and Assis) in São Paulo State. The following data were taken in these trials: number of days to flowering, yield, total plant height and height to pod insertion, flower color, pod pubescence and seed and plant growth type. Two of the mutants are of agronomic interest because they were, on average, seven to 10 days earlier in maturity than the control in all trials. Except for shorter plant height, the other agronomic characteristics remained unaltered.

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Effect of Gamma Irradiation of Local Brachiaria Ruziziensis (Germain & Evrard) Seeds on Agronomic Performance and Yield

Journal of Plant Breeding and Genetics ◽

10.33687/pbg.007.01.2836 ◽

2019 ◽

Vol 7 (1) ◽

pp. 09-17

Author(s):

Ann I. Hoka ◽

Michael Gicheru ◽

Syprine Otieno ◽

Hezekiah Korir

Keyword(s):

Dry Matter ◽

Gamma Ray ◽

Block Design ◽

Mutation Induction ◽

Agronomic Performance ◽

Brachiaria Ruziziensis ◽

Randomized Block Design ◽

Significant Difference ◽

Improved Performance ◽

Mutant Lines

Appropriate methods of mutation induction are of high importance in pastures for increased genetic variability and improved performance. The objective of this research was to improve the agronomic and nutritive performance of mutant lines (M7) through induced mutagenesis to seeds of a local landrace Brachiaria ruziziensis. The seeds were irradiated with 0, 10, 20, 30 and 40Gy doses of gamma radiation from Cobalt 60 (60Co). Treatments were; KE 0Gy, KE 10Gy, KE 20Gy, KE 30Gy and KE 40Gy resulting into M1 seeds. The M1 seeds were planted in the greenhouse in germination pots for one month and the seedlings transplanted to the field. Seeds of M6 plants (M7 seeds) were used to establish field experiment in a completely randomized block design, with three replications. Parameters measured included; tillering, leaf-stem ratio, dry matter weight, and seed weight. Data collected was analyzed using the SAS package. Mutant lines exhibited better agronomic performance compared to the wild type. Performance increased with increased gamma-ray exposure with 40Gy treatment outperforming all other treatments whereas the control performed dismally. There was a significant difference (P0.05) in the dry matter with 40Gy treatment having the highest values of dry matter yields, whereas control had the lowest values. Application of nuclear technology to other grasses would lead to increased biomass and improved nutrition for increased animal productivity leading to food and nutrition security.

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New wheat genotypes tolerant to aluminum toxicity obtained by mutation induction

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2001000100008 ◽

2001 ◽

Vol 36 (1) ◽

pp. 61-70 ◽

Cited By ~ 4

Author(s):

Augusto Tulmann Neto ◽

Marcelo Correa Alves ◽

Carlos Eduardo de Oliveira Camargo ◽

Jairo Lopes de Castro ◽

Wilson Penteado Ferreira Filho

Keyword(s):

Grain Yield ◽

Gamma Ray ◽

Aluminum Toxicity ◽

Acid Soils ◽

Field Trials ◽

Soil Conditions ◽

Mutation Induction ◽

Limiting Factor ◽

Agronomic Characteristics ◽

Mutant Lines

Seed from the sensitive wheat (Triticum aestivumL.) cultivar Anahuac was treated to gamma-ray irradiation and eleven Al3+ tolerant mutants selected. The objective was to compare these mutants to the original Anahuac and to the tolerant wheat cultivars IAC-24 and IAC-60 from 1994 to 1996 in acid (Capão Bonito) and limed (Monte Alegre do Sul) soil field trials, in the State of São Paulo, Brazil. Grain yield and agronomic characteristics were analyzed. All the mutant lines yielded higher than the sensitive Anahuac cultivar in the acid soils of Capão Bonito. Under limed soil conditions, 10 mutants had a similar yield to the original sensitive cultivar and one a lower yield. The majority of the mutants were similar in yield to the tolerant cultivars IAC-24 and IAC-60 under both conditions. Some of the mutants showed altered agronomic characteristics, but these alterations did not generally influence the grain yield. The results indicated that tolerant lines with good characteristics may be obtained from a susceptible cultivar by mutation induction, thus allowing cropping under conditions where Al3 + is a limiting factor.

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SELECTION OF SHORT STATURE AND EARLY MATURING BADSHAH BHOG IN M2 FROM GAMMA RAY IRRADIATION

PLANT ARCHIVES ◽

10.51470/plantarchives.2021.v21.no1.123 ◽

2021 ◽

Vol 21 (No 1) ◽

Author(s):

Zafar Imam ◽

Nihar Ranjan Chakraborty ◽

Jarman Gadi

Keyword(s):

Short Stature ◽

Plant Height ◽

Gamma Ray ◽

Rice Cultivar ◽

Treatment Result ◽

Early Maturing ◽

Gamma Ray Irradiation ◽

Late Maturity ◽

Productive Tiller ◽

Mutant Lines

Badshah Bhog is a non basmati aromatic traditional rice cultivar. which is not widely cultivated due to some unfavourable traits which were relatively high plant height, late maturity and low average yield. By the utilization of gamma ray irradiation in plant breeding attempted to overcome such problems in crops. The objective of this research is to observe and select M2 mutants of Badshah Bhog from gamma ray irradiation and obtain early maturing short stature plants. The research was conducted by Line sowing method. Established experimental plots for every irradiation doses and comparing the treatment result with the control to identify the effect of irradiation on the growth of Badshah Bhog. The result showed that there have been several plants with potential mutant traits supported the positive and negative character of every individual. The irradiated dose of 300 Grays has the shortest maturity duration of 131 days with the highest productive tiller of 6.67 but very low as compared to control. The irradiated dose of 400 Grays has the shortest Plant height of 97.8 cm. The plant yield of mutant lines in the present study was not affected significantly in any direction which could due to occurrence of polygenic mutations with ‘plus’ and ‘minus’ effect equally distributed. Together with short stature plants also plants bearing double spikelet at tip and mid region of panicle observed.

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Characterization of Irradiation Induced Mutants of Cassava (Manihot esculenta Crantz) Generated from Jame-jame and Adira-4 Genotypes at M1V2 Generation

KnE Life Sciences ◽

10.18502/kls.v2i6.1016 ◽

2017 ◽

Vol 2 (6) ◽

pp. 22

Author(s):

Nurul Khumaida ◽

Sintho Wahyuning Ardie ◽

Mira Sri Astuti

Keyword(s):

Plant Height ◽

Manihot Esculenta ◽

Gamma Ray ◽

Starch Content ◽

Tuber Weight ◽

Manihot Esculenta Crantz ◽

Carbohydrate Source ◽

The Stability ◽

Mutant Lines ◽

The Tropics

Cassava (Manihot esculenta Crantz., Euphorbiaceae) is an important dietary carbohydrate source for approximately 800 million people in the tropics. It is a potential crop for food, feed, and industry. Cassava breeding through conventional approaches are hampered with some limitations which resulted in a low number of superior varieties. The objective of this research was to generate several mutant lines with higher yield and starch content. This research intended to identify the second generation (M1V2) Jame-jame and Adira-4 variant cassava mutants from gamma ray irradiation. Jame-jame is a local genotype from Halmahera (local high yielding genotype, sweet, and potential) and Adira-4 is an Indonesia national variety of cassava. Mutant diversity appears on the plant height, plant branching, and plant tubers that harvested at 10 months after planting (MAP). Our results showed the positive correlation between plant height, first branching height, tuber weight, number of roots, and number of economic roots. Based on tuber weight and number of economic tuber there were 9 potential mutants (putative). Based on the stability test, there were 6 potential stable mutants (putative) by first branching height, tuber weight, total number of tubers, and total number of economies tuber, namely: V5D1-(2), V5D1-2(2), V5D1-3(2), V5D1-4(3), V5D2-2(2), and V5D2-6(2). Keywords: local genotype, M1V2, storage root, characters, stability.

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Morphological Variations in Buddleia Induced by Gamma Ray Irradiation

HortScience ◽

10.21273/hortsci.47.1.81 ◽

2012 ◽

Vol 47 (1) ◽

pp. 81-83 ◽

Cited By ~ 2

Author(s):

Wenhao Dai ◽

Victoria Magnusson

Keyword(s):

Gamma Rays ◽

Gamma Ray ◽

Flower Color ◽

Shoot Tips ◽

Mutation Induction ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Morphological Variations ◽

Gamma Ray Irradiation

Buddleia species is commonly used as a perennial for landscaping as a result of its heavy flowers and long bloom period. However, this species has a few concerns such as lack of flower color, excessive growth, and an invasive nature. Attempts to improve Buddleia using conventional breeding methods have resulted in limited success. In this study, mutagenesis by gamma ray irradiation was used to induce genetic variations. In vitro shoot tips of two Buddleia cultivars, B. davidii ‘Potters Purple’ and Buddleia ‘Lochinch’, were exposed to 0 to 150 Gy gamma rays and then recovered in Murashige and Skoog (MS) medium supplemented with 2.5 μM benzyladenine (BA). Shoots that recovered from the gamma ray treatment were rooted in half-strength MS medium with 0.5 μM naphthalene acetic acid (NAA) and grown in the greenhouse. The growth of shoot tips was inhibited after they were exposed to gamma rays. An average of 50.8% of shoots treated with 50 Gy gamma rays were recovered, whereas only 9.7% and 6.5% of shoots recovered when exposed to 100 and 150 Gy gamma rays, respectively. After transfer to the greenhouse, a few plants showed reduced growth with some dying before they reached the flowering stage. Various variations including characteristics of leaves (shape, size, hairs), stems (shape, internode length, branching), flowers (color, size, and structure), and plant stature were observed. This research demonstrates that in vitro mutation induction using gamma ray irradiation could be a useful protocol to develop new cultivars or genetic materials for further breeding of Buddleia or other related species.

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KERAGAMAN M1 TANAMAN HIAS BUNGA MATAHARI (Helianthus annuus L.) AKIBAT IRADIASI SINAR GAMMA

Journal of Agro Complex ◽

10.14710/joac.2.1.1-11 ◽

2018 ◽

Vol 2 (1) ◽

pp. 1

Author(s):

Intan Novela Setya Monikasari ◽

Syaiful Anwar ◽

Budi Adi Kristanto

Keyword(s):

Plant Height ◽

Seed Weight ◽

Gamma Ray ◽

Morphological Variability ◽

Flower Color ◽

Stem Diameter ◽

Initiation Time ◽

Flower Diameter ◽

Total Seed ◽

Lethal Doses

ABSTRACT The purpose of the research was to obtain the morphological variability of M1 sunflower ornamental plant and information of lethal doses (LD50) effect of mutation by gamma ray irradiation. The research was arranged in monofactor experimental with Completely Randomized Design (RAL) with 5 treatments and 5 replication, each replication consisted of 5 sunflower seeds was irradiated by gamma ray of 0, 5, 25, 45, and 65 Gy. The data observed were analyzed by anova and followed with BNT of 5% level. Parameters observed included germination, plant height, stem diameter, tube flower diameter, tape flower diameter, initiation time, perfect bloom age, chlorophyll, ribbon flower number, seed number, total seed weight, weight of 100 seeds, LD50, Flower color and heritability. The result showed that irradiation significant effected to plant height, stem diameter, tube flower diameter, ribbon flower diameter, initiation time, seeds number, seed weight, weight of 100 seeds. Lethal doses of sunflower is 74,19 Gy. Heritability value range from 23,47 to 73,81%.Keywords : Helianthus annus L., irradiation, LD50, heritability

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Response of growth and yield of two soybean varieties to seed pre-treatment applications

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/883/1/012052 ◽

2021 ◽

Vol 883 (1) ◽

pp. 012052

Author(s):

N Herawati ◽

A R Aisah ◽

I Mardian ◽

B N Hidayah ◽

B T R Erawati

Keyword(s):

Plant Height ◽

Seed Treatment ◽

Seed Quality ◽

Block Design ◽

Growth And Yield ◽

Agronomic Crops ◽

Randomized Block Design ◽

Two Factors ◽

Pre Treatment ◽

Four Levels

Abstract Plant growth is influenced by seed quality. Seeds need to be treated to prevent pest and disease disorders or to increase seed germination. Soybean planting was carried out in this study by the treatment of varieties and seed pre-treatment application before planting. The study aimed to measure the growth and yield of soybeans by varieties and seed treatment before planting, carried out in the Village of Nggembe, District of Bolo, Bima Regency. The study used factorial randomized block design with two factors. The first factor was soybean varieties consisting of two levels, namely Devon and Dena varieties, and the second factor was the application of pre-planting seed treatments consisting of four levels namely Cruiser, Marshall, Agrisoy, and without seed treatment, each treatment was repeated three times. Observations were made on the performance of agronomic crops and soybean yields. The results showed that the treatment of soybean varieties and the application of seed treatment had a significant influence on plant height and soybean productivity, and both treatments had interactions on parameters of plant height, number of filled pods, weight of 100 seeds, and soybean productivity with the best results successively produced by a combination of Devon-Control, Devon-Marshall, Devon-Agrisoy, and Dena-Cruiser.

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Polianthes tuberose Breeding in Indonesia

KnE Life Sciences ◽

10.18502/kls.v2i6.1081 ◽

2017 ◽

Vol 2 (6) ◽

pp. 587

Author(s):

Donald Sihombing

Keyword(s):

Gamma Ray ◽

Leaf Spot Disease ◽

Mutation Induction ◽

Induced Mutations ◽

Double Flower ◽

New Variety ◽

Local Cultivars ◽

Long Time ◽

New Varieties ◽

Flower Type

Tuberose (Polianthus tuberosa) is an important ornamental plant in Indonesia that has been cultivated by farmers for a long time mainly in Java and North Sumatra provinces. However, the availability of new superior varieties is only a few so that the consumers only have a limited choice. Until now, there are three types of tuberose in Indonesia, which is distinguished by the type of flower namely single, semi-double and double flower. Some breeding efforts to obtain new varieties have been done through crossbreeding, mutation induction and selection to local cultivars. But the success on the crossbreeding of tuberose was only about 0.05%, as it can only be done in the direction of the single flower with double flowers. Because in the double flowers pistils are not found, the variability is very low. Nevertheless from these crossbreeding obtained 29 genotypes that showed differences in several characters, especially in the arrangements of flowers; and flower stalks straightness and rigidness. The other study indicates that each genotype has a different resistance levels against leaf spot disease (Xanthomonas sP.). Meanwhile, breeding of tuberose through mutation induction technique has not been widely studied. Application of gamma ray irradiation on tubers caused morphological damaged on Polyanthes, reduced bulb growth less than 30% and reduced the plant height more 400% than no treated plants (control). Polyploidy induced mutations by using colchicine led to changes in the length and diameter of the flowers and harvesting time. Until now, there is no result of the crossbreeding and mutation techniques that released as a new variety. Despite this, based on the positive selection breeding on local cultivars, there have been two superior genotypes released as new varieties namely Dian Arum (double flower type; origin of Cianjur, West Java) and Roro (semi double flower type; origin of Pasuruan, East Java). Keywords: Polianthes tuberosa ; breeding; local cultivars, new varieties,Dian Arum, Roro Anteng

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Keragaman Mutan Wijen (Sesamum indicum L.) Berdasarkan Karakter Kualitatif; Diversity of Sesame Mutants (Sesamum indicum L.) Based on Qualitative Characters

Buletin Tanaman Tembakau Serat & Minyak Industri ◽

10.21082/btsm.v13n1.2021.1-13 ◽

2021 ◽

Vol 13 (1) ◽

pp. 1

Author(s):

Vina Eka Aristya

Keyword(s):

Genetic Variability ◽

Morphological Diversity ◽

Structural Diversity ◽

Morphological Characters ◽

Sesamum Indicum ◽

Mutation Induction ◽

Second Phase ◽

Genotype Distribution ◽

Qualitative Characters ◽

Mutant Lines

ABSTRAKSesamum indicum L. adalah salah satu tanaman alternatif penghasil minyak nabati penting. Pendekatan pemuliaan melalui induksi mutasi berusaha untuk menghasilkan variabilitas baru populasi wijen, yang secara umum tersusun dari individu homozigot. Karakter morfologi berguna untuk mengidentifikasi galur dan memastikan hasil pemuliaan mutasi. Tujuan penelitian ini adalah untuk mengevaluasi keragaman morfologi wijen hasil mutasi berdasarkan 26 karakter kualitatif. Penggalian informasi dengan metode analisis kelompok juga dikaji untuk menggambarkan variabilitas genetik pada 57 galur mutan wijen (jenis hitam dan putih) generasi M4 dan M5. Penelitian ini dilakukan dalam dua tahap: tahap pertama terdiri 18 galur generasi M4, dievaluasi pada Maret-Agustus 2015; tahap kedua terdiri atas 39 galur M5, ditanam pada November 2015 hingga April 2016. Pemeriksaan penampilan kualitatif mengikuti panduan descriptor list untuk wijen. Secara umum, penilaian representatif dari sifat kualitatif pada galur mutan wijen generasi M4 akan diikuti oleh keturunan generasi M5. Dendrogram dibangun untuk membedakan galur menjadi kelompok berdasarkan matriks tingkat kemiripan. Struktur populasi utama dari 57 galur berdasarkan sifat kualitatif dikategorikan dalam dua kelompok besar. Materi genotipe kelompok I diklasifikasikan menjadi dua sub-kelompok, terdiri 17 dan 31 galur. Sub-kelompok ini menjadi bagian distribusi genotipe terbesar. Kelompok II tersusun oleh 9 galur, mayoritas dari M4. Nilai korelasi antar karakter bervariasi antara 0,7176 hingga 1,0. Keragaman morfologi antar galur wijen dipengaruhi oleh sifat genetik dibandingkan faktor lingkungan. Studi ini membantu seleksi galur terpilih berdasarkan kestabilan fenotipe. Evaluasi keragaman struktur populasi wijen mutan bermanfaat untuk program pemuliaan.ABSTRACTSesamum indicum L. is one of the alternative crops that produces vegetative oil. The plant breeding approach through mutation induction could produce new genetic variability in sesame populations, which are generally composed of homozygous individuals. The study aims were to determine the diversity of sesame-mutant lines based on 26 qualitative characters. Cluster analysis method was carried out to describe the genetic variability of 57 sesame mutant lines (consist of black and white types) 4th (M4) and 5th generations (M5). The study was done in two phases, the first phases consisted of 18 M4 lines planted in March-August 2015; the second phase consisted 39 M5 lines planted in November 2015 to April 2016. Analyses of qualitative morphological characters followed the descriptors list for sesame. In general, a representative assessment of qualitative traits in M4 lines will be followed by the offspring of M5. Dendrogram showed that the 57 mutant lines categorized into two major clusters. Cluster I were composed of two sub-clusters, consisting 17 and 31 lines. This sub-cluster was the largest part of the genotype distribution. Cluster II composed of 9 lines, where M4 were the majority. The correlation value between characters ranged from 0.7176 up to 1.0. Morphological diversity among lines were largely influenced by genetic rather than environmental factors. This study supports the selection of lines based on phenotype stability. Evaluation of the structural diversity of mutant-sesame populations could be applied in sesame-breeding programs.

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Variabilitas Genotipe-Genotipe Mutan Krisan (Dendranthema grandiflora Tzvelv.) Generasi MV5 Hasil Irradiasi Sinar Gamma

Agrikultura ◽

10.24198/agrikultura.v27i3.10881 ◽

2016 ◽

Vol 27 (3) ◽

Author(s):

Dedeh Kurniasih ◽

Dedi Ruswandi ◽

Murdaningsih Haeruman Karmana ◽

Warid Ali Qosim

Keyword(s):

Experimental Method ◽

Plant Height ◽

Gamma Ray ◽

Phenotypic Variability ◽

Block Design ◽

Mutation Breeding ◽

Dendranthema Grandiflora ◽

Randomized Block Design ◽

Flower Diameter ◽

Flower Type

ABSTRACTVariability of mutant genotypes chrysanthemum (Dendranthema grandiflora Tzvelv.) fifth generationsthrough gamma iIrradiationDendranthema grandiflorum Tzvelv. is a major floriculture in Indonesia, and it is one of the five most popular flowers in Indonesia. Chrysanthemum varieties in Indonesia is largely the introduced varieties. Chrysanthemum hybridization especially for decorative flower type in order to obtain superior varieties is relatively difficult, so the mutation breeding is one approach that can be taken to get the chrysanthemum varieties with different phenotypic performances with the that parent.The purpose of this study was to obtain information genetic and phenotypic variability characters observed on chrysanthemum irradiated with gamma ray. The experiment was conducted by an experimental method using a randomized block design (RBD). The treatments consisted of 37 mutants genotypes and 11 genotypes chrysanthemums parent as controls with two replications. The results of this study indicated that the genotypes tested had broad genetic and phenotipic variation for the plant height, flower diameter, number of flower and neck lengths.Key words: Chrysanthemum mutants, Variability, Gamma ray irradiation.ABSTRAKKrisan merupakan komoditas tanaman hias utama di Indonesia dan paling banyak diminati masyarakat. Varietas-varietas krisan yang beredar di Indonesia sebagian besar merupakan varietas introduksi. Persilangan krisan khususnya untuk tipe bunga dekoratif dalam rangka memperoleh varietas unggul relatif sulit dilakukan, sehingga pemuliaan mutasi merupakan salah satu pendekatan yang dapat ditempuh untuk mendapatkan varietas krisan dengan penampilan fenotipik yang berbeda dengan induknya. Tujuan penelitian ini adalah untuk mendapatkan informasi variabilitas genetik dan fenotipik karakter-karakter yang diamati pada tanaman krisan yangd iradiasi dengan sinar gamma. Percobaan dilakukan dengan metode eksperimen menggunakan Rancangan Acak Kelompok (RAK). Perlakuan terdiri atas 37 genotipe mutan krisan dan 11 genotipe tetua krisan sebagai kontrol dengan dua ulangan. Hasil penelitian menunjukkan bahwa genotipe-genotipe yang diuji memiliki variabilitas yang luas untuk karakter tinggi tanaman, diameter bunga, jumlah kuntum dan panjang tangkai bunga.Kata kunci: Mutan krisan, Variabilitas, Sinar gamma

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