scholarly journals Changes in the volume and histology of endometriosis foci in rats treated with copaiba oil (Copaiferalangsdorffii)

2011 ◽  
Vol 26 (suppl 2) ◽  
pp. 20-24 ◽  
Author(s):  
João Nogueira Neto ◽  
Márcio Jorge de Souza Lindoso ◽  
Laura Fernandes Coelho ◽  
Rafael Antonio Freire Carvalho ◽  
Taciana Gabrielle Pinheiro de Moura Rodrigues ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Marked Reduction ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Control Group ◽  
Promising Alternative ◽  
Copaiba Oil ◽  
Experimental Group ◽  
Hematoxylin Eosin ◽  
Over Time

PURPOSE: The aim of this study was to analyze the changes that occur in rats with experimental endometriosis after treatment with copaiba oil. METHODS: Experimental endometriosis was induced in rats. The experimental group received copaiba oil (Copaiferalangsdorffii) orally (0.63 mg/day), and the control group received a 0.9% sodium chloride solution orally (1 ml/100 g of body weight/day). Both groups were treated with gavage for 14 days. After this period, the animals were euthanized, and the implant volume was calculated. The autologous transplants were removed, dyed with hematoxylin-eosin, and analyzed by light microscopy. RESULTS: The average final volumes were significantly different between the groups (p=0.007). There was a significant increase (p=0.012) between the initial and final volumes in the control group, whereas treatment with Copaiferalangsdorffii caused a marked reduction in endometrial growth over time (p=0.016). Histologically, 6/11 (55.00%) rats in the experimental group had a well-preserved epithelial layer, and 3 (45.00%) had mildly preserved epithelium. The control group had seven cases (58.30%) of well-preserved epithelial cells and five cases (41.70%) of mildly preserved epithelial cells (p>0.05). CONCLUSION: Copaiba oil (Copaiferalangsdorffii) appears to be a promising alternative treatment for endometriosis.

scholarly journals EVALUATION OF CUMULATIVE PROPERTIES OF THE COMPLEX TOOL Z-88

2021 ◽  
Vol 248 (4) ◽  
pp. 221-225
Author(s):  
V.G. Sofronov ◽  
◽  
Z.F. Aukhadieva ◽  
F.A. Medetkhanov ◽  
M.I. Gilemkhanov ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Saline Solution ◽  
Experimental Period ◽  
Control Group ◽  
Maximum Volume ◽  
Sterile Saline ◽  
Laboratory Rats ◽  
Experimental Group

This present work is devoted to the study of cumulative properties of the complex means Z-88. The studies were carried out on intact mongrel laboratory rats that had previously passed quarantine measures for 12 days. Two equivalent groups were created. The animals of the experimental group were injected intramuscularly in the thigh area with Z-88, and the control group was similarly injected with 0.9% sterile sodium chloride solution. On the first day of the experiment, the rats were injected with test drugs at a dose of 0.5 ml per animal, which was 1/10 of the maximum volume used in experiments to determine the parameters of acute toxicity. Both Z-88 and sterile saline solution were administered once a day throughout the experiment. Z-88 was administered once a day throughout the experiment. Starting from the fifth day, in every subsequent four days, the dose of the drug was increased by 1.5 times. The duration of the experiment was 24 days. During the experimental period of visible changes on the part of the studied indicators.

scholarly journals Salmonella Bacterin Vaccination Decreases Shedding and Colonization of Salmonella Typhimurium in Pigs

2021 ◽  
Vol 9 (6) ◽  
pp. 1163
Author(s):  
Eduarda Alexandra Gonçalves de Oliveira Moura ◽  
Daniela Gomes da Silva ◽  
Caio Henrique Turco ◽  
Thainara Vitoria Carnevalli Sanches ◽  
Gabriel Yuri Storino ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Blood Count ◽  
Control Strategies ◽  
Control Group ◽  
Blood Samples ◽  
Commercial Vaccine ◽  
Serum Igg ◽  
And Control ◽  
Experimental Group ◽  
Over Time

Since the occurrence of swine salmonellosis has increased over time and control strategies other than biosecurity are highly recommended, the present study aimed to evaluate the efficacy of vaccination with Salmonella Choleraesuis and Salmonella Typhimurium bacterins in pigs. Two experimental groups were formed: G1, animals immunized with two doses of a commercial vaccine (n = 20); G2, control group (n = 20). After vaccination, all pigs were orally challenged (D0) with 108 CFU of Salmonella Typhimurium and evaluated for 40 days. Every 10 days after D0, five piglets from each experimental group were euthanized and submitted to the necroscopic examination, when organ samples were collected. Blood samples and rectal swabs were collected before the first dose of the vaccine (D−42), before the second dose (D−21), before the challenge (D0), and thereafter, every three days until D39. Blood count, serum IgG measurement by ELISA, and the excretion of Salmonella Typhimurium in feces were evaluated. While the results from blood count and serum IgG concentration did not differ, the detection and excretion of Salmonella between G1 and G2 differed (p < 0.05). Therefore, it was observed that this vaccine partially protected the animals against experimental infection with Salmonella Typhimurium, reducing the excretion of bacteria in feces.

scholarly journals The effects of collaboration scripts on the number and quality of student interactions in a social annotation tool

2021 ◽  
Author(s):  
Vincent De Boer ◽  
Howard Spoelstra
Keyword(s):  
Control Group ◽  
Baseline Measurement ◽  
Student Interactions ◽  
Collaboration Scripts ◽  
Academic Texts ◽  
Active And Collaborative Learning ◽  
Social Annotation ◽  
Experimental Group ◽  
Over Time

Social Annotation (SA) tools can be used to facilitate active and collaborative learning when students have to study academic texts. However, making these tools available does not ensure students participate in argumentative discussions. Scaffolding students by means of collaborations scripts geared towards collaboration and discussion encourages students to engage in meaningful, high-quality interactions. We conducted an experiment with students (n=59) in a course running at a Dutch university, using the SA tool Perusall. A control group received normal instructions, while an experimental group received scaffolding through collaboration scripts. The results showed a significant increase in the number of responses to fellow students for the experimental group compared to the control group. The quality of the annotations, measured on levels of Bloom’s taxonomy, increased significantly for the experimental group compared to both its baseline measurement and the control group. However, when scaffolding was faded out over subsequent assignments these differences became non-significant. The experimental groups’ increased quality of annotations did not remain over time, suggesting that internalization of the scripts was not achieved.

scholarly journals Application of Magnetic Resonance Image Intelligent Data Acquisition in Ovarian Cancer (Preprint)

2020 ◽  
Author(s):  
Tingting Liang ◽  
Yanlei Dong ◽  
Xinrui Zhao ◽  
Lu Wang ◽  
Hui Xu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Magnetic Resonance ◽  
Epithelial Cells ◽  
Data Acquisition ◽  
Cell Lines ◽  
P53 Protein ◽  
Control Group ◽  
Ovarian Epithelial Cells ◽  
Intelligent Data Acquisition ◽  
Experimental Group

UNSTRUCTURED As a diagnostic method with no radiation, high resolution of soft tissue, and different imaging methods, Magnetic Resonance Imaging (MRI) intelligent data acquisition is more and more widely used in the examination of abdominal, pelvic, and other organ lesions. In order to study the diagnostic effect of multi-mode magnetic resonance intelligent data acquisition on ovarian cancer and the ovarian cancer model modified based on p53-/-+Myc+ASAP1 gene, NSG mice were selected as experimental subjects in this study. 293FT cell lines packaging p53, Myc, and ASAP1(ArfGAP with SH3 domain, Ankyrin repeat and PH domain 1) recombinant lentivirus were inoculated into mouse ovarian epithelial cells to construct mouse ovarian epithelial cell tumor cell lines and their performance was analyzed. According to the different injection cell lines, they were divided into the experimental group and the control group. Tumor samples were collected and the mice were analyzed using immunofluorescence staining and MRI. The results showed that, in the detection of protein expression in genetically modified cell lines, for p53-/-+Myc+ASAP1 fully modified cell lines, the high expression of ASAP1 and Myc functional proteins was detected after the lentivirus containing p53-/-, ASAP1, and Myc were introduced into mouse ovarian epithelial cells, while the expression of p53 protein decreased significantly; after inoculation into mice, it was found that the expression of ASAP1 protein and Myc protein in the experimental group was significantly higher than that in the control group, while the expression of p53 protein in the experimental group was significantly lower than that in the control group, with significant statistical difference; further MRI diagnosis of two groups of mice showed that the ADC (Apparent dispersion coefficient) value of the experimental group was significantly higher than the control group, there were statistically significant differences. Therefore, it was found that p53 gene expression was down-regulated and Myc and ASAPl genes were overexpressed in the tumor tissues and tumor cells formed, and tumor formation differences between the two groups of mice could be obviously found after MRI intelligent data acquisition, which provided experimental basis for early diagnosis of breast cancer in the later clinical stage.

Adhesion of Malassezia pachydermatis of different growth type to canine epithelial cells

2014 ◽  
Vol 17 (2) ◽  
pp. 365-366 ◽  
Author(s):  
M. Florek ◽  
J. Król ◽  
Z. Staroniewicz ◽  
B. Bażanów
Keyword(s):  
Vitamin E ◽  
Epithelial Cells ◽  
Glutathione Peroxidase ◽  
Dairy Cows ◽  
Clinical Mastitis ◽  
Control Group ◽  
Growth Type ◽  
Malassezia Pachydermatis ◽  
Blood Samples ◽  
Experimental Group

Abstract This study focuses on the effect of parenteral administration of Selenium (Se) and vitamin E on concentration of Se in plasma and the activity of glutathione peroxidase (GPx) in the blood of dairy cows during peripartal period and their effect on the reduction of clinical mastitis. From a 220 individuals Holstein herd in a two-four lactation-gestation cycle the control group (C), 1st (D1) and 2nd (D2) experimental group were selected. Every group consisted of 15 cows in the last phase of the pregnancy. All cows were fed with the diet containing 0.1 mg of Se per kg/DM. The blood samples from vena jugularis were collected approximately 21 days before calving (control sampling), 3 days, 12 days and 21 days after calving. On the day of control sampling and 12 days before calving in D1 group, cows were injected subcutaneously in the sprescapular region with preparation Selevit inj. a.u.v. at the doses of 48.4 mg/head of Se, and 550 IU/head of α-Tocoferol (α-Toc). In D2 group, cows were injected by the same preparation only on 21th day before calving with the same doses of Se and α-Toc. The increase in the concentration of Se in the plasma and activity GPx in blood in D1 group on the 3rd day and 12th day after calving were determined. Increase in plasmatic concentrations α-Toc on 3rd day after calving and reduction of occurrence of clinical mastitis (13.3%) as compared with control group were found

scholarly journals Effect of Aerobic Training on Cognitive Function and Arterial Stiffness in Sedentary Young Adults: A Pilot Randomized Controlled Trial

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Samuel Asamoah ◽  
Jason Siegler ◽  
Dennis Chang ◽  
Andrew Scholey ◽  
Alan Yeung ◽  
...  
Keyword(s):  
Young Adults ◽  
Cognitive Function ◽  
Arterial Stiffness ◽  
Pulse Pressure ◽  
Processing Speed ◽  
Aerobic Training ◽  
Augmentation Index ◽  
Control Group ◽  
Experimental Group ◽  
Over Time

This study measured cognitive and vascular responses to aerobic training in sedentary young adults. Ten adults (6 women, 4 men; 18–29 years) were randomly assigned to an experimental or no-treatment control group. The experimental group engaged in a 6-week intervention, performed on exercise cycle and treadmill, 3x/week, 50 min/session; intensity was increased over time. Outcome measures included arterial stiffness (augmentation index, AIx, and pulse pressure), cardiorespiratory fitness (), and cognitive function (attention, processing speed, working memory, episodic memory, and executive function). Participants randomized to aerobic training improved processing speed versus control (, ES = 0.55). However, no group × time effects were noted in other domains of cognitive function. AIx was reduced by approximately 16% from before to after intervention in the experimental group; however, the improvement was not statistically significant versus control (, ES = 0.22). Pulse pressure did not change between groups over time (, ES = 0.0). increased by approximately 10% in the experimental group; however, the change was not significant between groups over time (, ES = 0.27). Vascular and cognitive adaptations to aerobic training may move in parallel. Robust trials simultaneously investigating a broad spectrum of aerobic training interventions and vascular and cognitive outcomes are warranted.

Variations Across Time in Acoustic and Electroglottographic Measures of Phonatory Function in Women With and Without Vocal Nodules

1995 ◽  
Vol 38 (4) ◽  
pp. 783-793 ◽  
Author(s):  
Kelly Dailey Hall
Keyword(s):  
Duty Cycle ◽  
Vocal Fold ◽  
Signal To Noise Ratio ◽  
Control Group ◽  
Signal To Noise ◽  
Vocal Fold Vibration ◽  
Audio Recordings ◽  
Vocal Nodules ◽  
Experimental Group ◽  
Over Time

The purpose of this investigation was to identify variations over time in phonatory function of women with and without vocal nodules using acoustic and electroglottographic measures. Subjects were 10 women with vocal nodules (mean age=22.1, range=19–25) and 10 women with healthy larynges (mean age=25.0, range=18–32). Electroglottographic and audio recordings of speech were obtained for each subject over 3 consecutive days at three target times: morning, afternoon, and evening. Estimates of fundamental frequency (Hz), jitter (msec), shimmer (dB), and signal-to-noise ratio (dB) were made from a 1000 msec midportion of the vowel /α/ produced in a carrier phrase. In addition, a closed-to-open ratio was derived from the EGG duty cycle of the same 1000 msec segment and used to estimate timing characteristics of vocal fold vibration. The results showed no significant differences between the groups regarding a pattern of change in the acoustic or the EGG measures across times throughout the day. Furthermore, the experimental group demonstrated significantly lower closed-to-open ratios than the control group. With this exception, no other statistically significant differences between the groups were found.

scholarly journals Evaluation of the antitumour activity of local intraperitoneal hyperthermia in a model of advanced high-grade ovarian carcinoma in rats

2022 ◽  
Vol 20 (6) ◽  
pp. 78-87
Author(s):  
Ya. G. Murazov ◽  
A. L. Semenov ◽  
K. Yu. Senchik ◽  
A. O. Nyuganen ◽  
A. S. Artemyeva ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Ovarian Carcinoma ◽  
Chloride Solution ◽  
Room Temperature ◽  
Sodium Chloride Solution ◽  
Peritoneal Cancer Index ◽  
Rank Test ◽  
Control Group ◽  
Cytostatic Drug ◽  
Log Rank Test

In our study we carried out an exploratory assessment of the antitumor activity of hyperthermic intraperitoneal perfusion (Hipep) with 0.9 % sodium chloride solution and compared it with the effect of a single normothermic intraperitoneal (i.p.) Administration of cisplatin in the maximum tolerated dose (mtd). Thirty-six mature female Wistar rats with transplanted i.p. Syngeneic ovarian carcinoma were randomized into three groups: control group (2 ml of 0.9 % sodium chloride i.p. At room temperature, n=12); cisplatin group (cisplatin 4 mg/kg i.p. At room temperature, n=12); Hipep group (open i.p. Perfusion with 0.9 % sodium chloride solution at a temperature of 40,5–41,5 °c for 45 minutes, n=12). The primary endpoint was the overall survival (os) of the animals in each of the three groups. The total peritoneal cancer index (pci), weight and degree of ascites haemorrhagia were assessed at autopsy. The median os in the control group, Hipep, and cisplatin was 19, 39, and 40 days, respectively (log-rank test р<0.0001). In comparison to the control group, the differences were statistically significant for both cisplatin (HR=0.22; 95 % ci: 0.08–0.62; log-rank test р<0.0001) and Hipep (HR=0.32; 95 % ci 0.13–0.82; log-rank test р=0.0013). There were no differences in os between the cisplatin and Hipep groups (log-rank test р=0.4853). The Hipep procedure was associated with a significant decrease in total pci, a tendency towards a decrease in the ascites weight and a higher severity of haemorrhagia. In terms of os, local hyperthermia, provided by Hipep without a cytostatic drug, was comparable with single normothermic i.p. Administration of cisplatin in mtd and exceeded the effects of the latter in relation to the development of peritoneal carcinomatosis.

scholarly journals Results of Gemaza therapy of hemophthalmia in patients with diabetic retinopathy

2010 ◽  
Vol 13 (2) ◽  
pp. 97-99
Author(s):  
Nargiza Mirshavkatovna Normatova
Keyword(s):  
Diabetes Mellitus ◽  
Visual Acuity ◽  
Diabetic Retinopathy ◽  
Drug Therapy ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Control Group ◽  
Therapy Control ◽  
Traditional Drug ◽  
Hypoglycemic Therapy

Aim. To evaluate efficiency of Gemaza used to treat hemophthalmia of different severity and localization. Materials and methods. Gemaza (5000 IU) was admnistered parabulbarly to 75 patients (78 eyes) with diabetes mellitus (DM) after dilution in0.5 ml of 0.9% sodium chloride solution. Each patient received 10 daily injections during baseline hypoglycemic therapy. Control group was comprisedof 20 patients (20 eyes) with hemophthalmia, treated by traditional drug therapy (emoxipin, dicinon, ascorutin, actovegin). Results. Gemaza proved a highly efficient agent showing hemoresorptive and fibrinolytic activities; it improved visual acuity in 51.3% of the patientsfrom day 5 after the onset of therapy. Conclusion. It is recommended to administer Gemaza soon after hemorrhage, prior to the development of proliferative processes in the vitreous bodyand retina.

scholarly journals The Effect of Ginger on Preventing Post-spinal Puncture Headache in Patients Undergoing Cesarean Section

2018 ◽  
Vol 7 (2) ◽  
pp. 204-210
Author(s):  
Banafsheh Mashak ◽  
Maryam Hashemnejad ◽  
Kourosh Kabir ◽  
Mansoureh Refaei ◽  
Sara Esmaelzadeh Saeieh ◽  
...  
Keyword(s):  
Cesarean Section ◽  
Spinal Anesthesia ◽  
Repeated Measures ◽  
Control Group ◽  
Control Groups ◽  
Spinal Puncture ◽  
Comparison Results ◽  
And Control ◽  
Experimental Group ◽  
Over Time

Objectives: Post-spinal puncture headache (PSPH) has constantly been one of the research priorities, especially in women undergoing cesarean section (C-section) and it is related to physical and psychological problems. The present study aimed to evaluate the effect of ginger on preventing PSPH in patients undergoing C-section. Materials and Methods: This clinical trial was conducted on 160 women undergoing C-section with spinal anesthesia, who were eligible to enter the study in the experimental and control groups. One ginger capsule (250 mg) was prescribed every 8 hours (TDS) to the experimental group 24 hours before the C-section. The prescription of ginger was continued half an hour after transfer to the post-partum ward until the PSPH onset. PSPH intensity was measured by using visual analog scale (VAS) pain scores at 6 timepoints (Time 1=30, Time 2=60, and Time 3=90 minutes vs. Time 4=3, Time 5 =6, and Time 6=12 hours) after C-section. No interventions were performed in the control group. The data were analyzed using the SPSS software by descriptive statistics and analytical tests were applied to determine the changes in PSPH intensity. Results: The comparison results of the mean score of PSPH intensity in the experimental and control groups indicated significant differences over time (P<0.05), except for the sixth time point (12 hours after C-section). In addition, the trend of changes at 6 timepoints based on the results of the repeated-measures test demonstrated that PSPH intensity significantly differed in the two groups over time (P<0.001). Conclusions: Based on the result, the oral prescription of ginger to women undergoing C-section under spinal anesthesia led to effective PSPH prevention at 5 time-points (30, 60, and 90 minutes, along with 3 and 6 hours). Further, the trend of the changes represented that the intensity of PSPH decreased in the experimental group over time. Therefore, ginger is suggested as a noninvasive and efficient method used for preventing PSPH.

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