scholarly journals Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells

2012 ◽  
Vol 23 (1) ◽  
pp. 39-44 ◽  
Author(s):  
Camilla Christian Gomes Moura ◽  
Priscilla Barbosa Ferreira Soares ◽  
Karine Fernandes Carneiro ◽  
Maria Aparecida de Souza ◽  
Denildo Magalhães
Keyword(s):  
Mononuclear Cells ◽  
Control Cell ◽  
In Vitro Study ◽  
Negative Control ◽  
Collagen Membrane ◽  
Mitochondrial Activity ◽  
Proinflammatory Mediators ◽  
Collagen Membranes ◽  
Porcine Collagen

This study compared the cytotoxicity and the release of nitric oxide induced by collagen membranes in human mononuclear cells. Peripheral blood was collected from each patient and the separation of mononuclear cells was performed by Ficoll. Then, 2x10(5) cells were plated in 48-well culture plates under the membranes in triplicate. The polystyrene surface was used as negative control. Cell viability was assessed by measuring mitochondrial activity (MTT) at 4, 12 and 24 h, with dosage levels of nitrite by the Griess method for the same periods. Data had non-normal distribution and were analyzed by the Kruskal-Wallis test (p<0.05). Statistically significant differences (p<0.05) were observed between the membranes and the control in the experimental period, although there was a significant reduction in viability over time (p<0.01). At 4 and 12 h, the porcine membrane induced a higher release of nitrite compared with the control and bovine membrane, respectively (p<0.01), and this difference was maintained at 24 h (p<0.05). This in vitro study showed that the porcine collagen membrane induces an increased production of proinflammatory mediators by mononuclear cells in the first hours of contact, decreasing with time.

Fibronectin-adherent peripheral blood derived mononuclear cells as Paclitaxel carriers for glioblastoma treatment: An in vitro study

Cytotherapy ◽  
2017 ◽  
Vol 19 (6) ◽  
pp. 721-734 ◽  
Author(s):  
Marco Paolo Schiariti ◽  
Francesco Restelli ◽  
Paolo Ferroli ◽  
Anna Benetti ◽  
Angiola Berenzi ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Mononuclear Cells ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

2016 ◽  
Vol 45 (4) ◽  
pp. 234-239 ◽  
Author(s):  
Priscilla Barbosa Ferreira SOARES ◽  
Aletheia Moraes ROCHA ◽  
Manuella Verdinelli de Paula REIS ◽  
Camilla Christian Gomes MOURA ◽  
Carlos José SOARES
Keyword(s):  
Cell Viability ◽  
Tap Water ◽  
Human Fibroblasts ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Coconut Water ◽  
Ph Adjustment ◽  
Adjustment Method

Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

scholarly journals An In-Vitro Study of Molecular Effects of a Combination Treat-ment with Antibiotics and Nanofluid Containing Carbon Nano-tubes on Klebsiella pneumoniae

2021 ◽  
Author(s):  
Maryam Mehdizadeh ◽  
Mojgan Sheikhpour ◽  
Iman Salahshourifar ◽  
Seyed Davar Siadat ◽  
Parvaneh Saffarian
Keyword(s):  
Klebsiella Pneumoniae ◽  
Virulence Genes ◽  
Ultrasonic Method ◽  
Virulence Gene ◽  
In Vitro Study ◽  
Negative Control ◽  
Resistant Isolate ◽  
Simultaneous Treatment ◽  
Virulence Gene Expression

Background: We aimed to prepare a nanofluid, containing f-MWCNTs, and investigate the antibacterial efficacy of f-MWCNTs+ ciprofloxacin (cip) on Klebsiella pneumoniae by evaluating the virulence gene expression. Methods: This study was carried out from 2019 to 2020, in the Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran. The nanofluid containing antibiotic and f-MWCNTs were prepared by the ultrasonic method. The minimum inhibitory concentrations (MICs) of ciprofloxacin and f-MWCNTs were determined using the broth micro dilution MIC tests. For examining the antibacterial effects, the expression level of virulence genes, under the influence of f-MWCNTs, was evaluated by a real-time PCR. Results: The effect of 8 µg/ml ciprofloxacin + 400 µg/ml f-MWCNTs, completely inhibited the growth of the resistant isolate of K. pneumoniae, while, in the ATCC 700,603 isolate, 2 µg/ml ciprofloxacin with 100 µg/ml f-MWCNT could inhibit a bacterial growth. In the resistant K. pneumoniae clinical isolate, after f-MWCNT+cip treatment, the expression of fimA, fimD, wza, and wzi genes was significantly downregulated, compared to the ciprofloxacin treatment, and upregulated, compared to the negative control. For the ATCC 700,603 isolate treated with f-MWCNT+cip, the expression of fimA, fimD and wza virulence genes showed upregulation, compared to the negative control and downregulated in comparison with the ciprofloxacin treatment. Conclusion: Simultaneous treatment of resistant isolate of K. pneumoniae with f-MWCNTs +antibiotic could improve the effectiveness of antibiotic at lower doses, due to the reduced expression of virulence genes in comparison with antibiotic treatment, besides the increased cell wall permeability to antibiotics.

Comparative Evaluation of the Antimicrobial Effects of Different Mouthrinses against Streptococcus Mutans: An in Vitro Study

2019 ◽  
Vol 43 (6) ◽  
pp. 398-407
Author(s):  
Eman Abu-obaid ◽  
Fouad Salama ◽  
Ala’a Abu-obaid ◽  
Fars Alanazi ◽  
Mounir Salem ◽  
...  
Keyword(s):  
Reference Strain ◽  
In Vitro Study ◽  
Negative Control ◽  
Minimal Bactericidal Concentration ◽  
Distilled Water ◽  
Chlorhexidine Digluconate ◽  
Zone Of Inhibition ◽  
Antimicrobial Effects ◽  
Zones Of Inhibition

Purpose: To assess the antimicrobial effects of different natural and semi-natural mouthrinses on isolates of S. mutans obtained from the saliva of Saudi children and reference strains of S mutans (ATCC 25175). Study design: Saliva samples were collected from 20 children. Natural and semi-natural mouthrinses included were herbal mix mouthrinse, cranberry mouthrinse, chlorhexidine digluconate mouthrinse, cranberry extract mixed with chlorhexidine digluconate mouthrinse, chlorhexidine digluconate mouthrinse with alcohol (positive control), and distilled water (negative control). The microbiological examination tests were minimal inhibitory concentration, minimal bactericidal concentration, and zone of inhibition for the saliva isolates of S. mutans while zone of inhibition test only for reference strain of S. mutans. Results: For reference strain in a comparison with the distilled water, the herbal mix, cranberry, cranberry mixed with chlorhexidine, chlorhexidine, and chlorhexidine with alcohol showed significantly increased zones of inhibition by 36.38, 36.25, 26.13, 17.75, and 12.38, respectively. For saliva isolates in a comparison with the distilled water, the herbal mix, cranberry, cranberry mixed with chlorhexidine, chlorhexidine, and chlorhexidine with alcohol showed significantly increased zones of inhibition by 38.00, 34.25, 22.94, 16.50, and 16.44, respectively. Chlorhexidine with alcohol showed significantly lower minimum inhibitory and bactericidal concentration than the other groups. Conclusions: Herbal mix and cranberry mouthrinses could be effective natural alternative to chlorhexidine mouthrinse with or without alcohol in affecting tested parameters.

scholarly journals Degradation, Bone Regeneration and Tissue Response of an Innovative Volume Stable Magnesium-Supported GBR/GTR Barrier Membrane

2020 ◽  
Vol 21 (9) ◽  
pp. 3098 ◽  
Author(s):  
Mike Barbeck ◽  
Lennart Kühnel ◽  
Frank Witte ◽  
Jens Pissarek ◽  
Clarissa Precht ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Mononuclear Cells ◽  
Fibrous Tissue ◽  
Tissue Response ◽  
Collagen Membrane ◽  
Sham Operation ◽  
Barrier Membrane ◽  
Synthetic Materials ◽  
Native Collagen

Introduction: Bioresorbable collagenous barrier membranes are used to prevent premature soft tissue ingrowth and to allow bone regeneration. For volume stable indications, only non-absorbable synthetic materials are available. This study investigates a new bioresorbable hydrofluoric acid (HF)-treated magnesium (Mg) mesh in a native collagen membrane for volume stable situations. Materials and Methods: HF-treated and untreated Mg were compared in direct and indirect cytocompatibility assays. In vivo, 18 New Zealand White Rabbits received each four 8 mm calvarial defects and were divided into four groups: (a) HF-treated Mg mesh/collagen membrane, (b) untreated Mg mesh/collagen membrane (c) collagen membrane and (d) sham operation. After 6, 12 and 18 weeks, Mg degradation and bone regeneration was measured using radiological and histological methods. Results: In vitro, HF-treated Mg showed higher cytocompatibility. Histopathologically, HF-Mg prevented gas cavities and was degraded by mononuclear cells via phagocytosis up to 12 weeks. Untreated Mg showed partially significant more gas cavities and a fibrous tissue reaction. Bone regeneration was not significantly different between all groups. Discussion and Conclusions: HF-Mg meshes embedded in native collagen membranes represent a volume stable and biocompatible alternative to the non-absorbable synthetic materials. HF-Mg shows less corrosion and is degraded by phagocytosis. However, the application of membranes did not result in higher bone regeneration.

scholarly journals Tigecycline Interferes with Fibrinogen Polymerization Independent of Peripheral Interactions with the Coagulation System

Antibiotics ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 84 ◽  
Author(s):  
Anna Brandtner ◽  
Mirjam Bachler ◽  
Dietmar Fries ◽  
Martin Hermann ◽  
Jacqueline Ruehlicke ◽  
...  
Keyword(s):  
Qualitative Difference ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Coagulation System ◽  
Mitochondrial Activity ◽  
Hepatic Cells ◽  
Spiked Plasma ◽  
Fibrin Network ◽  
Coagulation Tests

Tigecycline offers broad anti-bacterial coverage for critically ill patients with complicated infections. A described but less researched side effect is coagulopathy. The aim of this study was to test whether tigecycline interferes with fibrinogen polymerization by peripheral interactions. To study the effect of unmetabolized tigecycline, plasma of healthy volunteers were spiked with increasing concentrations of tigecycline. In a second experimental leg, immortalized human liver cells (HepG2) were treated with the same concentrations to test an inhibitory effect of hepatic tigecycline metabolites. Using standard coagulation tests, only the activated thromboplastin time in humane plasma was prolonged with increasing concentrations of tigecycline. Visualization of the fibrin network using confocal live microscopy demonstrated a qualitative difference in tigecycline treated experiments. Thrombelastometry and standard coagulation tests did not indicate an impairment of coagulation. Although the discrepancy between functional and immunologic fibrinogen levels increased in cell culture assays with tigecycline concentration, fibrinogen levels in spiked plasma samples did not show significant differences determined by functional versus immunologic methods. In our in vitro study, we excluded a direct effect of tigecycline in increasing concentrations on blood coagulation in healthy adults. Furthermore, we demonstrated a rapid loss of mitochondrial activity in hepatic cells with supra-therapeutic tigecycline dosages.

Evaluating Dentin Surface Treatments for Resin-Modified Glass Ionomer Restorative Materials

2013 ◽  
Vol 38 (4) ◽  
pp. 429-438 ◽  
Author(s):  
TA Imbery ◽  
A Namboodiri ◽  
A Duncan ◽  
R Amos ◽  
AM Best ◽  
...  
Keyword(s):  
Testing Machine ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Surface Treatments ◽  
Band Matrices ◽  
Dentin Surface ◽  
Occlusal Surfaces ◽  
Significant Difference

SUMMARY This in vitro study evaluated the effect of six surface treatments on the shear bond strength of three resin-modified glass ionomers (RMGIs) to dentin. Occlusal surfaces of caries-free third molars were reduced to expose only dentin. Surface treatments were smear layer intact (negative control), Cavity Conditioner, EDTA, Ketac Primer, Self Conditioner, and etching with 35% phosphoric acid followed by the application of Optibond Solo Plus. Filtek Z250 composite resin bonded with Optibond Solo Plus served as a positive control. Conditioning agents were used according to the manufacturers' instructions. After surface treatments, Fuji II LC, Riva LC, Ketac Nano, and Filtek Z250 were placed in copper-band matrices 5 mm in diameter and 2 mm in height and were light-cured for 20 seconds. Specimens were stored in 100% humidity for 24 hours, after which they were placed in deionized water for 24 hours at 37°C. They were then tested under shear forces in an Instron Universal Testing Machine at a crosshead speed of 0.5 mm/min. A two-way analysis of variance and Tukey honestly significant difference statistical analyses (p&lt;0.05) indicated significant interaction between RMGIs and conditioning agents. Acid etching followed by Optibond Solo Plus provided highest bond strengths for all three RMGIs, which were not statistically different from the positive control.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

scholarly journals In Vitro Evaluation of Antimicrobial Activity of Coffee Grounds Extracts against Fish Pathogenic Aeromonas hydrophila

2020 ◽  
Vol 151 ◽  
pp. 01042
Author(s):  
Wahyu E. Sari ◽  
Muhammad Hambal ◽  
Henni Vanda ◽  
Maryulia Dewi ◽  
Rumi S. Zamzami ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
In Vitro Study ◽  
Negative Control ◽  
Diffusion Method ◽  
Natural Antimicrobials ◽  
Alternative Source ◽  
Coffee Grounds ◽  
Coffee Ground ◽  
Ethanol Extracts

Fish diseases caused bypathogenic bacteria Aeromonas hydrophila is one of the obstacles for fish farming because it can cause death and loss for farmers. A. hydrophila was reported to possess resistance to several antibiotics. Therefore, there is a need to seek an alternative source of natural antimicrobials. This in vitro study aimed to examinethe effect of ethanol extracts of coffee grounds as an antimicrobial to A. hydrophila which infects carp (Cyprinus carpio). The coffee grounds were collected from four traditional coffee shops in Banda Aceh, and a total of 500 g coffee grounds were extracted using 96% ethanol. Antimicrobial assay of ethanol extracts of coffee grounds was performed by the agar well diffusion method on Mueller Hinton Agar (MHA) plates. The result showed that all coffee ground extracts at concentration of 10%, 50%, and 100% were able to inhibit A. hydrophila bacteria with the highest inhibition zone of 10.96 mm posses a strong inhibition, while negative control does not indicate the existence of clear zone. The experiment confirmed the efficacy of coffee ground extracts as natural antimicrobials and suggested the possibility of utilizing them as a drugs for treatment of fish pathogenic caused by A. hydrophila.

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