scholarly journals Ligation of the left renal vein in epm1-wistar rats: functional and morphologic alterations in the kidneys, testes and suprarenal glands

1997 ◽  
Vol 115 (4) ◽  
pp. 1475-1484 ◽  
Author(s):  
José Carlos Costa Baptista-Silva ◽  
Miriam Sterman Dolnikoff ◽  
Luiz Antonio Ribeiro Moura ◽  
José Osmar Medina Pestana ◽  
Jose Gilberto Henriques Vieira ◽  
...  
Keyword(s):  
Renal Vein ◽  
Wistar Rats ◽  
Normal Values ◽  
Statistical Significance ◽  
Left Renal Vein ◽  
Serum Testosterone ◽  
Control Group ◽  
Male Adult ◽  
Initial Surgery ◽  
Suprarenal Glands

OBJECTIVE: The ligation of the left renal vein (LLVR) in man is a contraversial procedure in view of the risks of lesion to the renal parenchyma. With the objective of studying the morphologic and functional alterations caused by these lesions, we conducted experimental research with rats. MATERIAL AND METHODS: 64 male adult EPM1-WISTAR rats were used, divided into 8 groups - 4 for LLRV and four for control. Each LLRV group and corresponding control group were sacrificed progressively on the 7th, 15th, 30th and 60th day after the initial surgery. RESULTS: We found morphofunctional alterations only in animals that underwent LLRV in the four periods of sacrifice.The proteinuria creatinine in serum, testosterone in serum and serum corticosterone in serum showed practically no alteration in relation to the normal values for rats. Statistically significant severe histological lesions were found in the kidneys and testes of the LLRV groups. Lesions in the suprarenal glands were also present in these groups, but no sufficient to demonstrate statistical significance CONCLUSION: Based on these results we can conclude that the ligation of the left renal vein is a procedure of high risk in these animals.

scholarly journals Effects of ligature-induced periodontitis in pregnant Wistar rats

2003 ◽  
Vol 17 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Mariane Ponzio de Azevedo Galvão ◽  
Cassiano Kuchenbecker Rösing ◽  
Maria Beatriz Cardoso Ferreira
Keyword(s):  
Body Weight ◽  
Birth Weight ◽  
Low Birth Weight ◽  
Periodontal Disease ◽  
Wistar Rats ◽  
The Body ◽  
Control Group ◽  
Female Adult ◽  
Pregnant Rats ◽  
Male Adult

The aim of this study was to evaluate the influence of ligature-induced periodontal disease in pregnant rats on their newborn's health parameters. Twenty-four female adult Wistar rats were divided into two groups: the control group (G1) and the group that was submitted to dental ligatures around second upper molars (G2). After the four week period of development of periodontitis, the female animals were mated with male adult Wistar rats. There were no differences in the body weight of females between the two groups during mating and pregnancy. No differences were observed among the groups in relation to the viable newborn index. However, there were differences in newborn birth weight, explained by the diverse size of the litters. In this study, ligature-induced periodontal disease did not promote changes during pregnancy that resulted in low birth weight in newborn Wistar rats.

scholarly journals The effect of vitamin C on Endosulfan-induced oxidative stress parameters in prepubertal rats

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 333-338
Author(s):  
Kalaivani Manokaran ◽  
Vasanthalaxmi Krishnananda Rao ◽  
Nilima . ◽  
Manjula Shimoga Durgoji Rao ◽  
Sucheta Prasanna Kumar
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Treated Group ◽  
Reproductive Organs ◽  
Protective Role ◽  
Control Group ◽  
Group I ◽  
Testicular Weight

Introduction and Aim: Oxidative stress plays a very important role in endosulfan-induced toxic effects on reproductive organs. Vitamin C is a potent antioxidant which plays an important role in decreasing oxidative stress. The present study was aimed to investigate the protective role of vitamin C against endosulfan-induced testicular toxicity in Wistar rats. To investigate a protective effect of vitamin C against endosulfan induced toxicity on biochemical changes. Materials and Methods: Seventy male neonatal Wistar rats were divided into  seven groups. The group  I was taken as the control group, the endosulfan-treated were grouped into II (3 mg/kg body weight (BW) and group III (6 mg/kg BW), Group IV (9 mg/kg BW) and Group V (12 mg/kg BW). Group VI (9 mg/kg BW) and group VII (12 mg/kg BW) were pretreated with vitamin C (20 mg/kg BW) for 60 days. After  the experimental procedures, the testicular weight, lactate dehydrogenase (LDH) enzyme and testosterone in plasma, LDH, steroidogenic enzymes 3?-HSD and 17?-HSD in testis were evaluated. One-way ANOVA was used to determine the statistical significance. Results: Significant improvement in the testicular weight (P<0.05) , LDH (P<0.05) levels both in plasma and testis, increase in testosterone(P<0.001) and steroidogenic enzyme levels(P<0.001) was observed in the group pretreated with vitamin C treated group when compared to the endosulfan treated group. Conclusion: Vitamin C decreases the toxic effect of endosulfan on testis. The present action might be  due to its antioxidative properties.

scholarly journals Hypoglycemic and Toxic Effect of Morus mesozygia Leaf Extract on the Liver and Kidneys of Alloxan-Induced Hyperglycemic Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Michael Tirwomwe ◽  
Isaac Echoru ◽  
Richard Maseruka ◽  
Kyobe Ronald Kimanje ◽  
Wilson Byarugaba
Keyword(s):  
Toxic Effect ◽  
Total Protein ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Statistical Significance ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Histological Damage ◽  
Alloxan Monohydrate ◽  
Antidiabetic Effects

Purpose. We investigated the hypoglycemic and toxic effect of Morus mesozygia leaf extract on the liver and kidneys of alloxan-induced hyperglycemic wistar rats. Method. Phytochemical analysis was done. Diabetes was induced by the use of alloxan monohydrate in six groups of rats, i.e., 200 mg/kg, 400 mg/kg, 800 mg/kg, glibenclamide, normal saline, and normal control group. Blood glucose was measured at the time of inoculation, then at 1, 2, 3, and 4 hours after. After 14 days, rats were killed under anesthesia; blood collected for measurement of total protein, albumin, TAGs, cholesterol, AST, ALT, urea, and creatinine; and whole tissue of liver and kidneys used for histological studies. Results. The extract possessed antidiabetic effects between 400 mg/kg and 800 mg/kg doses, which we attributed to the presence of flavonoids, tannins, terpenoids, and amino acids. There was a drop in total protein and albumin with no statistical significance (P≥0.05). The changes in levels of ALT, TAGs, cholesterol, AST, creatinine, and urea were not statistically different from the standard diabetic drug. The extract was protective against histological damage as there were no significant lesions suggestive of toxicities in the liver and kidneys at doses below 800 mg/kg. Conclusion. We established credible evidence that Morus mesozygia leaf extract has hypoglycemic effects between 400 mg/kg and 800 mg/kg and that it is safe on the liver and kidneys of wistar rats at doses less than 800 mg/kg.

scholarly journals Pesticides, insecticides and male infertility

2017 ◽  
Vol 6 (8) ◽  
pp. 3387 ◽  
Author(s):  
Priyanka Roy ◽  
Pranay Kumar Phukan ◽  
Debojit Changmai ◽  
Surajeet Boruah
Keyword(s):  
Semen Analysis ◽  
Statistical Significance ◽  
Endocrine Disrupting Chemicals ◽  
Sperm Concentration ◽  
Serum Testosterone ◽  
Exposed Group ◽  
Control Group ◽  
Infertile Male ◽  
Insecticide Exposure ◽  
Male Patients

Background: Pesticides, insecticides can damage the male reproductive system in a number of ways. They may cause reproductive toxicity with direct damage to the structure of the cells or as a result of biotransformation into metabolites. They can alter DNA structure. They may also act like hormones in the endocrine system and disrupt the function of the natural endogenous hormones, when doing so they are often called endocrine disrupting chemicals. The objective of the study was to evaluate the association of infertility with pesticide and insecticide exposure among the infertile male patients attending Out Patient Department of Assam Medical College and Hospital.Methods: The pesticides and insecticides used by the population were cypermethrin, thiaclopride, acetamiprid, emmacetin benzoate A total of 55 oligospermic infertile male patients were taken for the study. Twenty-seven among them were exposed to pesticides and rest twenty-eight unexposed cases were taken as control group. Semen analysis was done along with serum FSH, LH and Testosterone. Scrotal ultrasonography of the subjects was also done. One way ANOVA test and t-test assuming two unequal variances were applied to find out the statistical significance of the association of pesticides with their infertility. p<0.05 was taken to be significant.Results: A significant decrease in sperm concentration was found in the exposed group. Also, a significant increase in serum LH was found in the exposed group. A significant decrease in sperm concentration and serum testosterone along with a significant increase in serum FSH and LH was found with increase in duration of exposure to pesticides and insecticides.Conclusions: The gonadotoxic effect of pesticides and insecticides was reflected from the study and it was concluded that pesticide and insecticide exposure has got a definite relation with the infertility of the subjects evaluated in the study.

Kinins in relation to renin activity in renal and inferior caval veins in normal individuals and patients with primary aldosteronism

1981 ◽  
Vol 96 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Lennart Hulthén ◽  
Kjell Jonsson ◽  
Harry Lecerof ◽  
Bernt Hökfelt
Keyword(s):  
Primary Aldosteronism ◽  
Renal Vein ◽  
Statistical Significance ◽  
Left Renal Vein ◽  
Plasma Renin ◽  
Renin Activity ◽  
Renal Veins ◽  
Plasma Aldosterone Concentration ◽  
Normal Individuals ◽  
The Right

Abstract. Blood kinin concentration and plasma renin activity (PRA) were determined in the renal veins and in the inferior caval vein (ICV) below the level of the renal veins in 9 normal individuals (5 men and 4 women) and 7 patients with primary aldosteronism (3 men and 4 women). PRA in the renal veins and ICV was significantly lower and plasma aldosterone concentration in ICV significantly higher in the patients as compared to the normals. Kinin concentration in the renal veins and ICV did not differ significantly between the patients and the normals (0.31 ± 0.04 (mean ± sem) vs 0.45 ± 0.03 and 0.27 ± 0.05 vs 0.45 ± 0.06 μg/l, respectively). In the normal individuals kinin concentration and PRA were inversely related in the left renal vein (r = −0.80; P < 0.02), whereas this relation did not reach statistical significance in the right renal vein (r = −0.60; P < 0.10). Kinin concentration in both renal veins was positively correlated to kinin concentration in ICV (r = 0.73; P < 0.05 and r = 0.83; P < 0.02 for the right and left renal vein, respectively). These results indicate that intrarenal kinin formation and renin release are inversely related in normal subjects. Furthermore, they suggest that one and the same enzyme, possibly renal kallikrein, may be implicated in the kinin formation in the systemic as well as in the renal circulation. Intrarenal kinin formation seems not to be increased in primary aldosteronism.

scholarly journals ANÁLISE DOS EFEITOS DA EXPOSIÇÃO CRÔNICA ORAL AO HERBICIDA À BASE DE GLIFOSATO NA REMODELAÇÃO CARDÍACA EM RATOS

2020 ◽  
Vol 12 (3) ◽  
pp. 10-15
Author(s):  
Larissa Ferreira Ros ◽  
Cristiane Pontes Teodozio ◽  
Ana Julia Alias Costa ◽  
Renata Calciolari Rossi ◽  
Rejane Batista Brinholi ◽  
...  
Keyword(s):  
Left Ventricle ◽  
Right Ventricle ◽  
Cardiac Remodeling ◽  
Wistar Rats ◽  
Experimental Period ◽  
Control Group ◽  
Male Adult ◽  
Herbicide Glyphosate

The aim of this study was to analyze the structural cardiac remodeling of rats exposed to the herbicide glyphosate chronically. Twenty male adult Wistar rats (300-450 g) were used, divided into a control group (CG, n=10) and group of rats fed diet contaminated by glyphosate (OG), n =10) for 180 days. After the experimental period, the rats were euthanized and to evaluate structural cardiac remodeling the heart was dissected in the left ventricle (LV), atriums (ATs) and right ventricle (RV) and heavy. There was an increase in the atrium (CG= 0,084±0,22mg e OG 0,105±0,21mg, p=0,048), right ventricles (CG= 0,219±0,04mg e OG= 0,276±0,03mg, p=0,036) and left ventricles (CG= 0,950±0,05mg e OG= 1,030±0,09mg, p=0,040) in the group exposed to glyphosate. Glyphosate promoted an increase in the cardiac chambers of rats that ingested contaminated feed.

scholarly journals The effect of X-ray irradiation to the formation of polychromatic erythrocyte cell micronucleus in Wistar rats (Rattus norvegicus)

2021 ◽  
Vol 54 (3) ◽  
pp. 128
Author(s):  
Eha Renwi Astuti ◽  
Hutojo Djajakusuma ◽  
Indeswati Diyatri ◽  
Nastiti Faradilla Ramadhani
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Treatment Plan ◽  
Statistical Significance ◽  
Clinical Parameter ◽  
Control Group ◽  
X Ray ◽  
Polychromatic Erythrocytes ◽  
Male Wistar Rats ◽  
Group 2

Background: Panoramic and cephalometric radiography is very important for diagnosis, treatment plan, and evaluation of orthodontic treatment results. Panoramic and cephalometric radiography are frequently performed at the same time, causing DNA damage and chromosome aberration. Purpose: This study aims to analyse the effect of X-ray exposure in panoramic and cephalometric radiography on micronuclei cell numbers. Methods: Laboratory-based analytical study with 60 healthy-male Wistar rats weighing 200–300 grams divided into 6 treatment groups (n=10). The control group: without radiographic exposure, the treatment group 2: using panoramic radiographic exposure followed by cephalometric, and the treatment group 3: using panoramic radiographic exposure and 24 hours later performed cephalometric radiographic. The unit of analysis was the polychromatic erythrocytes of mice cell, were examined 24 hours and 48 hours after irradiation had been finished. The polychromatic erythrocytes were examined using May-Gruenwald-Giemsa staining and 100x magnification under a microscope with 2000 cells per view. Data obtained were analysed using the SPSS 20 version software. The mean and standard deviations were calculated for each clinical parameter, and a one‐way ANOVA statistical test of significance was used. Statistical significance was set at p<0.05. Results: The analysis showed a significant increase (p<0.05) in the number of micronucleus in groups that used panoramic radiographic exposure followed by cephalometric. Conclusion: X-ray radiation can increase the number of micronucleus in polychromatic erythrocyte cells in rats.

scholarly journals Cadmium induced hepatic intoxication and amelioration by grape seed extract

2017 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Adel Adel Alkhedaide ◽  
Gehan Youssef ◽  
Rabab El-Zoghby ◽  
Mohamed Mahmoud ◽  
Shimaa Atwa
Keyword(s):  
Wistar Rats ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Seed Extract ◽  
Degenerative Changes ◽  
Control Group ◽  
Central Vein ◽  
Stomach Tube ◽  
Male Adult ◽  
Hydropic Degeneration

Cadmium (Cd) is wide-spread toxic metal that pollutes most of the vegetables, which eaten by numerous populations all over the world. The aim of the current work is to evaluate the protective and prophylactic effects of the antioxidant materials in the grape seed extract (GSE) on the hepatic intoxication induced by cadmium chloride toxic material in male Wistar rats. Male adult Wistar rats were divided into four groups. Control group fed on balanced diet and given drinking water. Group two (control positive) given CdCl2 in the dose of 0.44 mg/kg body weight (BW) by stomach tube daily. Group three given grape seed extract GSE in the dose of (100 mg/kg BW) daily by stomach tube. Finally, the fourth group gave mixture of (GSE and CdCl2) by stomach tube in the recommended dose. Blood and liver tissues were collected for further biochemical and histopathological studies. CdCl2 significantly increased the serum levels of malondialdehyde, ALT and AST. Cadmium administration decreased levels of antioxidants (Catalase, GSH-R and GSH-Px). The liver of the control positive groups which given CdCl2 showed degenerative changes in the form of vacuolar and hydropic degeneration. Congestion was evident in the central vein and proliferation of the kupffer cells. These hepatic biochemical and degenerative changes were ameliorated by the co addition of GSE.

scholarly journals Gestational Exposure to Synthetic Steroid Hormones Impaired Sperm Quantity and Quality in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Samy Ismail Ahmed ◽  
Aamir Magzoub ◽  
Mohammed Saeed Zayed Al-Ayed ◽  
Gamal Ali Attia ◽  
Basel A. Abdel-Wahab ◽  
...  
Keyword(s):  
Prenatal Exposure ◽  
Wistar Rats ◽  
Sperm Count ◽  
Test Group ◽  
Serum Testosterone ◽  
Reproductive Hormones ◽  
Control Group ◽  
Gestational Exposure ◽  
Male Wistar Rats ◽  
And Function

This study was designed to investigate the effect of prenatal exposure to synthetic sex steroid on sperm quantity and quality, relative testicular and epididymal weights, and reproductive hormones level in adult Wistar rats. Forty male Wistar rats were divided into two groups: a test group (n = 20) that included mature rats that were born to dams exposed to gestational treatment with hydroxyprogesterone and a control group (n = 20) that included mature rats born to untreated dams. Compared to the control group, the test group showed a significant reduction in the sperm count, viability and motility, relative testicular and epididymal weights together with increased abnormal spermatozoa (p<0.001). The reproductive hormonal assay revealed significantly lower serum testosterone and higher levels of FSH and LH among the test groups compared to the control (p<0.05 for all). Prenatal exposure to synthetic progesterone negatively affected sperm production and function, relative testicular and epididymal weights, and reproductive hormone levels.

scholarly journals Action of tacrolimus on Wistar rat kidneys implanted with Walker 256 carcinosarcoma

2010 ◽  
Vol 25 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Cristiano Machado Inácio ◽  
Ulrich Andréas Dietz ◽  
Osvaldo Malafaia ◽  
Jurandir Marcondes Ribas Filho ◽  
Paulo Afonso Nunes Nassif ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Statistical Significance ◽  
Control Group ◽  
Walker 256 Carcinosarcoma ◽  
Tumor Group ◽  
Male Wistar Rats ◽  
Walker 256 ◽  
The Right

PURPOSE: To evaluate the development of Walker 256 tumor in male Wistar rats treated with tacrolimus using an experimental kidney tumor model. METHODS: 40 male Wistar rats were divided into four groups: Tumor group (TU) (n=10), Tacrolimus-Tumor group (TT) (n=10), Tacrolimus group (TC) (n=10) and Control group (C) (n=10). Treatment with tacrolimus was performed in groups TT and TC. Under anesthesia, the right kidney of each animal of TU and TT was accessed through a supraumbilical incision and inoculated with a 0.1mL solution containing 2x10(6) tumor cells (Walker 256 carcinosarcoma tumor cells). Group TC was treated with a saline solution. All the animals of groups TC and TT were treated with tacrolimus (5mg/kg/day) by gavage for 15 days. TU group animals received saline by gavage for 15 days. On the 15th postoperative day, all animals were submitted to euthanasia and blood sampling for analysis of serum creatinine (Cr) and blood urea nitrogen (BUN). Abdominal gross examination was performed, the right kidney removed and prepared for histological analysis by hematoxylin-eosin staining. The resulting data were submitted to statistical analysis by ANOVA. RESULTS: Statistical significance was found when comparing creatinine level between groups TU, TT and TC -TT group culminated with a marked increased in creatinine levels (Cr=1.013 ± 0.3028 mg/mL), TU group (Cr=0.5670 ± 0.03536 mg/dL) P=0.00256, TC group (Cr =0.711 ± 0.1653 mg/mL) P= 0.02832. Statistical significance was found when comparing BUN levels in TT group (71.32 ± 17.14 mg/mL), compared with TU group (45.83 ± 5.046 mg/dL), P=0.000318. There were no statistically significant differences between groups TT and TC (61.23 ± 9.503 mg/mL) P=0.7242. Histological analysis showed a poor evolution in TT group with multiple foci of hemorrhage and cortical invasion by the Walker tumor. CONCLUSION: The Tacrolimus-treated group developed a more aggressive tumor and a drug-related nephrotoxic effect.

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