Cadmium induced hepatic intoxication and amelioration  by grape seed extract

Cadmium (Cd) is wide-spread toxic metal that pollutes most of the vegetables, which eaten by numerous populations all over the world. The aim of the current work is to evaluate the protective and prophylactic effects of the antioxidant materials in the grape seed extract (GSE) on the hepatic intoxication induced by cadmium chloride toxic material in male Wistar rats. Male adult Wistar rats were divided into four groups. Control group fed on balanced diet and given drinking water. Group two (control positive) given CdCl2 in the dose of 0.44 mg/kg body weight (BW) by stomach tube daily. Group three given grape seed extract GSE in the dose of (100 mg/kg BW) daily by stomach tube. Finally, the fourth group gave mixture of (GSE and CdCl2) by stomach tube in the recommended dose. Blood and liver tissues were collected for further biochemical and histopathological studies. CdCl2 significantly increased the serum levels of malondialdehyde, ALT and AST. Cadmium administration decreased levels of antioxidants (Catalase, GSH-R and GSH-Px). The liver of the control positive groups which given CdCl2 showed degenerative changes in the form of vacuolar and hydropic degeneration. Congestion was evident in the central vein and proliferation of the kupffer cells. These hepatic biochemical and degenerative changes were ameliorated by the co addition of GSE.

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Postorthodontic Relapse Prevention by Administration of Grape Seed (Vitis vinifera) Extract Containing Cyanidine in Rats

European Journal of Dentistry ◽

10.1055/s-0039-3401440 ◽

2019 ◽

Vol 13 (04) ◽

pp. 629-634 ◽

Cited By ~ 3

Author(s):

Ananto Ali Alhasyimi ◽

Niswati Fathmah Rosyida ◽

Mufliha Santi Rihadini

Keyword(s):

Wistar Rats ◽

Alveolar Bone ◽

Gingival Crevicular Fluid ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Orthodontic Appliances

Abstract Objective The aim of this study was to analyze the effect of grape seed extract containing cyanidin on osteoclastogenesis (by means of receptor activator of nuclear factor-κ B ligand [RANKL] and osteoprotegerin [OPG] levels) and the number of osteoclasts during orthodontic relapse in Wistar rats. Materials and Methods This study is an in vivo quasi experimental research. A total of 32 male Wistar rats were used in the study, which were randomly split equally into two groups, grape seed (GS) and control group (CG). All rats were given an orthodontic force of 35 cN using a stainless steel 3-spin coil spring that was activated for 7 days and then conditioned to be passive. During this phase, the GS group was administered grape seed extract containing cyanidin once per day. Orthodontic appliances were removed from both groups afterward, and then the alveolar bone tissue was isolated consecutively according to observation days (days 1, 3, 7, and 14), while OPG and RANKL levels were analyzed in their gingival crevicular fluid using a specific enzyme-linked immunosorbent assay (ELISA). Tissues were then stained with hematoxylin–eosin (H&E) and observed under a light microscope to count the number of osteoclast cells. Data were analyzed statistically using an independent t-test (p < 0.05). Results The number of osteoclasts in the GS group was significantly lower than that in the CG group on all experiment days (p = 0.021; p = 0.001; p = 0.024; p = 0.001; p < 0.05). ELISA results showed that the RANKL level of the GS group was significantly lower on days 3 and 7 (p = 0.025; p = 0.039; p < 0.05), while the OPG level was significantly higher on days 1 and 3 in the GS group than in the CG group (p = 0.039; p = 0.021; p < 0.05). Conclusion Grape seed extract can prevent postorthodontic relapse movement by inhibiting osteoclastogenesis and reducing the number of osteoclasts in Wistar rats.

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Noise Exposure Decreases the Number of Spermatozoa in Male Mice (Mus musculus L.) Even With Grape Seed Extract (Vitis vinifera L.) Administration

WMJ (Warmadewa Medical Journal) ◽

10.22225/wmj.5.2.1785.70-75 ◽

2020 ◽

Vol 5 (2) ◽

pp. 70-75

Author(s):

Ghestiara Siregar

Keyword(s):

Treatment Group ◽

Mus Musculus ◽

Noise Exposure ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Control Group ◽

Vitis Vinifera L ◽

Not Given ◽

Male Mice

Excessive continuous noises exposure changes the male hormone system which leads to formation of oxidative stress and results in disrupt of semen quality. This condition can be reduced by the use of antioxidants. Grape seed is one of the antioxidants that contains phenol components that have Resveratrol compounds. This research aimed to observe the effect of noise exposure on the number of spermatozoa of male mice given grape seed extract. The method of the research was a post-test only control group design with research subjects of 30 male Mus musculus L. (Swiss Webster) mice divided into 5 groups: group A (treatment control) was not given noise exposure and grape seed extract, group B (negative control) was not given noise exposure and was given grape seed extract, groups C, D, E (treatment group) were given noise exposure with sequential intensities of 65 dB, 85 dB, 105 dB. The treatment was given for 33 days. The results showed that noise exposure with different intensities of 65 dB, 85 dB, 105 dB reduced the spermatozoa count of male Swiss Webster mice even with the administration of grape seed extract. One Way Anova test was used to analyze the data with p-value of 0.001. Conclusion: There were differences in spermatozoa count between the control group and the treatment group. Provision of noise exposure with a value above the threshold limit reduces the number of male Swiss Webster mice spermatozoa given with grape seed extract. Keywords: Number of Spermatozoa, Grape Seed Extract, Noise exposure

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Effects of Grape Seed Extract, Vitamin C, and Vitamin E on Ethanol- and Aspirin-Induced Ulcers

Advances in Pharmacological Sciences ◽

10.1155/2011/740687 ◽

2011 ◽

Vol 2011 ◽

pp. 1-6 ◽

Cited By ~ 7

Author(s):

Vivian Molina Cuevas ◽

Yazmín Ravelo Calzado ◽

Yohani Pérez Guerra ◽

Ambar Oyarzábal Yera ◽

Sonia Jiménez Despaigne ◽

...

Keyword(s):

Lipid Peroxidation ◽

Gastric Ulcer ◽

Vitamin E ◽

Grape Seed Extract ◽

Grape Seed ◽

Positive Control ◽

Negative Control ◽

Seed Extract ◽

Gastric Ulcers ◽

Control Group

Effects of GSE and vitamins C and E on aspirin- and ethanol-induced gastric ulcer and associated increases of lipid peroxidation in rats were compared. Two experiments were conducted. Rats were randomized into eight groups: a negative control and seven groups that received aspirin or ethanol for ulcer induction: one positive control (vehicle) and six with VC, VE, or GSE (25 and 250 mg/kg). Ulcer indexes and gastric levels of malondialdehyde (MDA) were quantified. VC, VE, and GSE (25 and 250 mg/kg) decreased aspirin, and ethanol-induced ulcers and MDA values compared with positive control group. The magnitude of aspirin ulcer reduction was comparable for all treatments, and MDA decrease with GSE was higher than with VC and tended to be greater, albeit none significantly, than with VE. GSE was more effective than VC and VE for lowering the ethanol ulcers, while the decrease of MDA levels with GSE was greater than with VC, but comparable to that achieved with VE. GSE protected against ethanol-induced gastric ulcers more effectively than VC or VE, while its protection against aspirin ulcers was comparable for all treatments. GSE produced the greatest reductions of gastric MDA in both models.

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Upregulation of Antioxidant Gene Expressions and Enzyme Activity Against Acrylamide-Induced Neurotoxicity in Mice after Grape Seed Extract Treatment

The Open Biotechnology Journal ◽

10.2174/1874070702014010023 ◽

2020 ◽

Vol 14 (1) ◽

pp. 23-31

Author(s):

Sarah Albogami

Keyword(s):

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Control Group ◽

Gene Expressions ◽

Neuroprotective Effects ◽

Treatment Groups ◽

Biochemical Assays ◽

Test Parameters

Background: The risk of occupational exposure to acrylamide is high and long-term acrylamide exposure can cause neurotoxicity. Thus, therapeutic agents that can protect against acrylamide-induced neurotoxicity are needed. Objective: To investigate whether Grape Seed Extract (GSE) protects against acrylamide-induced neurotoxicity in mice. Methods: Mice were divided into saline, GSE, acrylamide, GSE followed by acrylamide, acrylamide followed by GSE, and simultaneous acrylamide and GSE treatment groups. Gene expression and antioxidant enzyme levels were then determined using RT-PCR and biochemical assays. Results: Gpx1 (P < 0.05), Prdx3 (P < 0.01), SOD1 (P < 0.05), and CAT (P < 0.05) significantly upregulated in GSE-treated mice, compared to those in untreated controls. In contrast, Gpx1 (P < 0.05), Prdx3 (P < 0.05), SOD1 (P < 0.05), and CAT (P < 0.05) significantly downregulated in acrylamide-treated mice compared to those in untreated controls. Results of the treatment with GSE before exposure to acrylamide or simultaneously with acrylamide indicated that GSE restored Gpx1, Prdx3, SOD1, and CAT expression to similar levels as those in the control group. GSE treatment after exposure to acrylamide did not exert any neuroprotective effects against acrylamide, as revealed by significant downregulation of Gpx1 (P < 0.05), Prdx3 (P < 0.01), SOD1 (P < 0.05), and CAT (P < 0.05) compared to that in untreated controls. Animals treated with grape seed before acrylamide treatment showed no significant change in LPO activities and a significant increase in GSH levels, compared to those in untreated controls. Conclusion: GSE exerted neuroprotective effects against acrylamide-induced neurotoxicity. Acrylamide caused oxidative stress 20 days post-exposure. However, grape seed treatment before exposure to acrylamide restored all test parameters to levels similar to control values.

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The Effects of Grape Seed Extract on Insulin, Adiponectin and Resistin Levels in Diabetic Rats

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i4.709-713.4023 ◽

2021 ◽

Vol 9 (4) ◽

pp. 709-713

Author(s):

İnci Söğütlü ◽

Nihat Mert ◽

Handan Mert ◽

Leyla Mis ◽

Halil Cumhur Yılmaz ◽

...

Keyword(s):

Insulin Level ◽

Grape Seed Extract ◽

Grape Seed ◽

Diabetic Rats ◽

Seed Extract ◽

Control Group ◽

Albino Rats ◽

Diabetes Group ◽

Positive Effects ◽

Wistar Albino Rats

In this study, the effects of grape seed extract on insulin, adiponectin and resistin levels in diabetic rats were aimed to investigate. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats were used. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats randomly divided into four groups of eight each: control group (C), group with diabetes (DM), grape seed extract group (GSE), diabetes mellitus and grape seed extract group (DM+GSE). The diabetic group 45 mg/kg single dose of streptozotocin was administered intraperitoneally, and the grape seed extract groups (DM+GSE and GSE) grape seed extract was given orally every day with an intragastric tube for 20 days (0.6 ml/rat). Blood samples were taken from the hearts of rat’s end of the experiment. The sera obtained were used for insulin, adiponectin and resistin analysis measured via ELISA with commercial kits. The insulin and adiponectin levels of the rats in the diabetes + grape seed extract group were found to be higher than those in the diabetes group. There was no significant change in the resistin level differences between the groups. As a result; It is possible to say that grape seed extract has an increasing effect on insulin level and adiponectin levels of diabetic rats are decreased due to diabetes and it has no significant effect on resistin levels. Detected by the positive effects of grape seed extracts, it may be useful to use for the regulating of the diabetes in human.

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Grape Seed and Physalis Extracts as Anti- Inflammatory Agents in Cetuximab Induced Tongue Mucositis in Rats

Journal of Natural Remedies ◽

10.18311/jnr/2020/25070 ◽

2020 ◽

Vol 20 (3) ◽

pp. 173

Author(s):

Heba A. Saleh ◽

Manar A. Abdul Aziz ◽

Mona E. Wali

Keyword(s):

Mast Cells ◽

Cancer Therapy ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Male Rats ◽

Control Group ◽

Tissue Preparation ◽

Group I ◽

Area Percent

Background: Oral mucositis induced by cancer therapy alters cancer patient’s life. Nutraceuticals these days is for their various activities which could neutralize the toxicity induced by cancer treatment. Purpose: Comparision between the effect of grape seed and fruits of Physalis peruviana methanol extract in reduction of mucositis induced by Cetuximab drug. Study Design: Animal study. Methods: 60 male rats divided into 6 groups as 10 in each group. Control group of rats with no intervention given. The other 5 groups were given Cetuximab drug alone or with grape seed extract (simultaneously or 1 week before Cetuximab drug) or Physalis extract (simultanously or 1 week before Cetuximab drug). After sacrifice, tongue was dissected and tissue preparation was done for all samples. Digital counting was done by image analyzer computer system for (a) filliform papillae in H&E stained sections, (b) mast cells in mast cell tryptase stained sections to assess inflammation. The area percent for bax immune-expression in other immune stained sections were also measured done to assess apoptosis. Results: The difference between the groups was assessed for statistical significance (P<0.0001) by ANOVA test. The greatest mean number of filiform papillae was recorded in control group I. Greatest mean number of mast cells and the greatest mean area percent of bax immune expression were recorded in group II. Conclusion: Grape seed extract and Physalis extract could neutralize the harmful effect of cancer therapy. Physalis extract had more potent effect in reducing the inflammation induced by Cetuximab drug.

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Role of Grape Seed Extract on Methotrexate Induced Oxidative Stress in Rat Liver

The American Journal of Chinese Medicine ◽

10.1142/s0192415x08006302 ◽

2008 ◽

Vol 36 (05) ◽

pp. 861-872 ◽

Cited By ~ 46

Author(s):

Aysun Çetin ◽

Leylagul Kaynar ◽

Ismail Kocyigit ◽

Sibel Kabukcu Hacioglu ◽

Recep Saraymen ◽

...

Keyword(s):

Oxidative Stress ◽

Rat Liver ◽

Blood Count ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Control Group ◽

Radical Scavenger ◽

Distilled Water ◽

Deep Anaesthesia

The efficacy of methotrexate (MTX), a widely used cytotoxic chemotherapeutic agent, is often limited by its severe hepatotoxicity. Regarding the mechanisms of these adverse effects, several hypotheses have been put forward, among which oxidative stress is noticeable. The present study was undertaken to determine whether grape seed extract (GSE), a new natural free radical scavenger, could ameliorate the MTX-induced oxidative injury in the rat liver. The animals were divided into 3 groups. Each group consisted of 12 animals. MTX-GSE group: rats were given GSE (100mg/kg body weight) orally for 15 days, and a single dose of MTX (20mg/kg, intraperitoneally) was added on the 10th day. MTX group: these received placebo distilled water (orally) instead of GSE for 15 days and the same MTX protocol applied to this group on the 10th day. Control group: rats were given distilled water (orally) through 15 days and physiological saline (intraperitoneally) instead of MTX was administered on the 10th day in a similar manner. On the 16th day, liver tissue samples were obtained under deep anaesthesia. The level of malondialdehyde (MDA), an end product of lipid peroxidation, and the activities of süperoxide dismutase (SOD) and catalase (CAT), two important endogenous antioxidants, were evaluated in the tissue homogenates. MTX administration increased the MDA level and decreased the SOD and CAT activities in the liver homogenates ( p < 0.001), while these alterations were significantly reversed by GSE treatment ( p < 0.001). MTX led to significantly reduced whole blood count parameters ( p < 0.05). When GSE was supplemented, no significant changes in blood count parameters were noted. It appears that GSE protects the rat liver and inhibits methotrexate-induced oxidative stress. These data indicate that GSE may be of therapeutic benefit when used with MTX.

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Effect of Grape Seed Extract in Minimizing the Harmful Effects of Monosodium Glutamate on the Chicken Embryonic Retina to Show Scientific Miracles In the Holy Quran

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2020/v32i2530819 ◽

2020 ◽

pp. 7-18

Author(s):

Fawzya Al- Gamdi ◽

Nouf Al- Harbi

Keyword(s):

Negative Impact ◽

Monosodium Glutamate ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

The Body ◽

Control Group ◽

Beverage Industry ◽

Potential Improvement ◽

Grape Seed Extracts

In 1969, a Canadian laboratory announced that its studies on synthetic alkaline (cyclamate) proved to be a carcinogen despite its use for more than twenty years in the beverage industry. There are many additives that have raised the controversy about the large extent of safety to consumers and the most famous controversy and consumption is the article of monosodium glutamate (MSG) which touched research on the negative impact on samples of research experiments. This research aims to study the effect (MSG) on the formation of retina in chicken embryos and the potential improvement effects of GSE grape seed extracts during incubation as part of the solutions provided in the event of the long term use of MSG and its accumulation in the body Human. The experimental groups were divided into 4 groups based on the injection period and the injected substances. The first control group(Injected with saline), the second group, treated with MSG, the 3rd group were treated with Monosoudim glutamate and grape seed extract at days 0,1 of incubation, whereas the 4thgroup,was treated with MSG and then the GSE (M – G) were given at3,4 days post MSG . The effect of the experimental materials on the development of the retina at the age of 14 was investigated in the current work. The results showed that the treatment of embryos with MSG dose (0.1 ml) caused many of the abnormalities at the level of tissue formation of the retina, and after the treatment of the fetus with GSE was noted to reduce the damage to the tissue of the retina. The results showed that the treatment of embryos with MSG dose (0.1 ml) caused many of the abnormalities at the level of tissue form of the retina, and after the treatment of the fetus with GSE was noted to reduce the damage to the tissue of the retina.

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Grape Seed Proanthocyanidins Attenuate Anxiety-Like Behavior in an Experimental Model of Dietary-Induced Hypercholesterolemia in Rats

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000286 ◽

2015 ◽

Vol 85 (5-6) ◽

pp. 282-291

Author(s):

Zienab Alrefaie

Keyword(s):

Open Field ◽

Elevated Plus Maze ◽

Grape Seed Extract ◽

Grape Seed ◽

Seed Extract ◽

Control Group ◽

High Cholesterol ◽

Grape Seed Proanthocyanidins ◽

Cholesterol Levels ◽

Plus Maze

Abstract. Objective: Grape seed proanthocyanidins have many health-protective effects. The present work aimed to assess their possible anxiolytic effects in dietary-induced hypercholesterolemia in rats. Methods: 30 Male Wistar rats were divided into 3 groups (n = 10): control (normal chow), high cholesterol (cholesterol and cholic acid chow) and high cholesterol + grape seed extract (hypercholesterolemic chow + grape seed extract, 100 mg/kg body weight/day). After 4 months, total cholesterol levels and animals’ behavior in the open field and elevated plus maze were assessed. Results: High-cholesterol diet elevated cholesterol levels (p < 0.001 vs control group), however, grape seed administration ameliorated this elevation (p < 0.001 vs high-cholesterol untreated group). In the open field, hypercholesterolemic rats showed increased periods of immobility (138 ± 20.3 seconds (s) vs 96.9 ± 14.5 in control rats p < 0.001) and time latency to enter field center (109 ± 18.3 s compared to 40.8 ± 10.5 s in the control group p < 0.001). Both parameters were reduced by grape seed treatment (p < 0.01, p < 0.001 vs the untreated hypercholesterolemic group, respectively). Elevated plus maze testing demonstrated higher closed-arm entries (5.5 ± 1.1 in hypercholesterolemic animals vs 3 ± 1.1 in the control group p < 0.001) and lower percentage of time spent in open arms (19.7 ± 3.5 % vs 35.8 ± 4.8 % in control rats p < 0.001). Grape seed extract significantly enhanced the percentage of time spent in open arms (p < 0.001 compared to hypercholesterolemic rats). Conclusion: The present results highlight the possible anxiolytic effect of grape seed proanthocyanidins in dietary-induced hypercholesterolemia.

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