scholarly journals Effect of dietary supplementation with propolis and Aloe barbadensis extracts on hematological parameters and parasitism in Nile tilapia

2015 ◽  
Vol 24 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Geovana Dotta ◽  
Aline Brum ◽  
Gabriela Tomas Jeronimo ◽  
Marcelo Maraschin ◽  
Maurício Laterça Martins
Keyword(s):  
Stress Level ◽  
Nile Tilapia ◽  
Hematological Parameters ◽  
Dietary Supplementation ◽  
Aloe Barbadensis ◽  
Blood Samples ◽  
Gill Parasites ◽  
Water Tanks ◽  
Hematological Alterations ◽  
Sampling Times

This study evaluated the influence of diet supplementation with propolis and Aloe barbadensis on hematological parameters and parasitism in tilapia. One hundred and eighty fish were distributed among 12 water tanks forming four treatments: fish supplemented with a 1:1 mixture of 0%, 0.5%, 1% and 2% propolis and aloe extracts. After the fish had been fed on the experimental diets for 15 and 21 days, blood samples were taken and parasites collected. The monogeneans Cichlidogyrus sclerosus, C. halli, C. thurstonae and Scutogyrus longicornis were identified in the gills. Between the sampling times, there were increases in the numbers of erythrocytes, leukocytes, thrombocytes and lymphocytes, as observed after 21 days, possibly due to the stress level over the course of the assay and/or accumulation of substances in the organism. Supplementation with the mixture of propolis and aloe for 15 days showed the highest efficacy against the parasites. This was possibly due to the association between the two compounds. The results demonstrated that supplementation with mixtures of extracts did not produce hematological alterations and also favored a significant reduction in the number of gill parasites. The best results were achieved after 15 days of feeding with a diet with 0.5% and 1% supplementation with the extract mixture, which increased efficiency by 83 and 85% respectively

scholarly journals Seroprevalence of Toxoplasma gondii and associated hematological alterations in small ruminants of D.G. Khan district of Southern Punjab, Pakistan

2020 ◽  
Vol 72 (5) ◽  
pp. 1698-1704
Author(s):  
M.H. Lashari ◽  
U. Farooq ◽  
S. Mubeen ◽  
W. Hassan ◽  
M.F. Azhar ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Blood Cells ◽  
Hematological Parameters ◽  
Age Groups ◽  
Small Ruminants ◽  
Blood Samples ◽  
Elisa Kit ◽  
Hematological Effects ◽  
And Gender ◽  
Hematological Alterations

ABSTRACT The present study was carried out to evaluate the prevalence and hematological effects of Toxoplasma gondii in sheep and goat in district Dera Ghazi Khan. Blood samples (n=204) were collected comprise goats (n=101) and sheep (n=103) alongwith age, gender and breeds of animals. Samples were collected randomly from 25 flocks of 7 different union council Viz. Vehova, Tibbi Qaisrani, Lakhani, Kohar, Tuman Qaisrani, Nutkani and Kot Qaisrani of Tehsil Taunsa Sharif at least 4 animals from each flock. All ruminants divide into three groups based on age, breed and gender. The prevalence was detected through two different kits Viz. LAT and ELISA kit. The overall prevalence suspected in goats through LAT and ELISA kit was (35.64%), (32.67%) and in sheep was (25.24%), (23.30%) respectively. The Toxoplasma gondii had a significant effect on goats in age groups and non-significant all other groups of goats and sheep. Toxoplasma gondii had a significant effect on all hematological parameters like Hemoglobin, total leukocyte cells, granulocytes, lymphocytes, platelets, and red blood cells, except monocytes. In conclusion of the current study, toxoplasmosis is prevalent among ruminants, reveals the possibility of transmission to humans on the use of host animals as protein source.

Dietary supplementation of dry oregano leaves increases the innate immunity and resistance of Nile tilapia against Streptococcus agalactiae infection

2019 ◽  
Vol 51 (2) ◽  
pp. 418-436 ◽  
Author(s):  
Amanda H. Espirito Santo ◽  
Túlio S. Brito ◽  
Laura L. Brandão ◽  
Guilherme C. Tavares ◽  
Márcia P. Leibowitz ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Streptococcus Agalactiae ◽  
Nile Tilapia ◽  
Dietary Supplementation

scholarly journals Acute inflammation and hematological response in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

2015 ◽  
Vol 75 (2) ◽  
pp. 491-496 ◽  
Author(s):  
G. Dotta ◽  
J. Ledic-Neto ◽  
ELT. Gonçalves ◽  
A. Brum ◽  
M. Maraschin ◽  
...  
Keyword(s):  
Immune Response ◽  
Inflammatory Response ◽  
Nile Tilapia ◽  
Acute Inflammation ◽  
Leukocyte Count ◽  
Acute Inflammatory Response ◽  
Swim Bladder ◽  
Aloe Barbadensis ◽  
Natural Extracts ◽  
Number Of Cells

This study evaluated the acute inflammatory response induced by carrageenin in the swim bladder of Nile tilapia supplemented with the mixture of natural extracts of propolis and Aloe barbadensis (1:1) at a concentration of 0.5%, 1% and 2% in diet during 15 days. Thirty-six fish were distributed into four treatments with three replicates: fish supplemented with 0.5% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 1% of admix of extracts of propolis and Aloe (1:1) injected with 500 µg carrageenin; fish supplemented with 2% of admix of extracts of propolis and Aloe (1:1), injected with 500 µg carrageenin and unsupplemented fish injected with 500 µg carrageenin. Six hours after injection, samples of blood and exudate from the swim bladder of fish were collected. It was observed an increase in the leukocyte count in the swim bladder exudate of fish supplemented with extracts of propolis and Aloe injected with carrageenin. The most frequent cells were macrophages followed by granular leukocytes, thrombocytes and lymphocytes. Supplementation with propolis and Aloe to 0.5% caused a significant increase in the number of cells on the inflammatory focus mainly macrophages, cells responsible for the phagocytic activity in tissues, agent of innate fish immune response.

scholarly journals Influence of Pesticide on Biochemical and Hematological Parameters in Beninese Vegetable Farmers

2018 ◽  
Vol 9 (1) ◽  
pp. 65
Author(s):  
Lucie AYI-FANOU
Keyword(s):  
Blood Cell ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Hematological Parameters ◽  
Control Group ◽  
Study Group ◽  
Blood Samples ◽  
Vegetable Farmers ◽  
Insignificant Difference ◽  
Hematological And Biochemical Parameters

In several countries, a lot of researches have shown the toxicity of pesticides on farmers. Meanwhile, few of them have dealt with vegetable farmers in Benin. Therefore, this study has been carried out to assess the effects of pesticides on vegetable farmers’ health. This study is conducted on 30 vegetable farmers who used pesticides and 20 subjects as control group. Pesticides used by vegetable farmers and their risky behavior were investigated. Their blood samples were collected and some tests were performed for hematological and biochemical parameters. For the statistical analysis of the results, Student’s test was used. Our investigations revealed that vegetable farmers of Benin were exposed to different active ingredients of pesticides such as (Abamectin, Acetamiprid, Chlorpyriphos ethyl, Cyfluthrin, Cypermethrin, Emamectin benzoat, Flubendiamid, Lambdacyhalothrin, Mancozeb, Profenofos, Spinosad and Spirotétramate). The hematological parameters showed significant decrease in White blood cell (WBC), Red blood cell (RBC), Hemoglobin (HGB), Hematocrit (HTC), Mean Corpuscular Volume (MCV), Platelet (PLT) count among study group. The enzymatic activities of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) have significantly raised between vegetable farmers and the control group, while the serum concentration in urea and creatinine showed insignificant difference in the study group. These results suggest that pesticides have adverse effects on vegetable farmers of South of Benin.

scholarly journals Evaluation of Anthelmintic Efficacy of Ivermectin, Levamisole HCl and Albendazole Administered through Different Routes against Naturally Occuring Gastrointestinal Nematodiasis in Black Bengal Goat Inducing Hematological Parameters and Live Weight Indices

2016 ◽  
Vol 5 (1) ◽  
pp. 26-34 ◽  
Author(s):  
M Aktaruzzaman ◽  
MS Islam ◽  
MMI Hasan ◽  
MJU Bhuiyan ◽  
MM Hossain ◽  
...  
Keyword(s):  
Body Weight ◽  
Hematological Parameters ◽  
Live Weight ◽  
Gastrointestinal Nematodes ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Counting Method ◽  
Blood Samples ◽  
Group A ◽  
Group D

This study aimed to evaluate the efficacy of ivermectin, levamisole HCl and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study included 52 black Bengal breed of which 30 were naturally infected and randomly selected 20 on the basis of their weight and egg count. Twenty black Bengal goats of 12-13 month old irrespective of sex infested with gastrointestinal nematodes were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 goats and goats of group D were kept as control group. One injectable ivermectin (200?gkg-1 body weight, S/C) preparations (Techno Drugs Ltd. Bangladesh) and two solid levamisole HCl, albendazole (7.5 mgkg-1 body weight, orally) preparations (The ACME Laboratories Ltd. and Square Pharmaceuticals Ltd. Bangladesh) were used for positive control of gastrointestinal nematodes as group A, B and C. Goats of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the fecal and blood samples were collected directly from rectum and examined on 7th, 14th, 21st and 28th day using McMaster fecal egg counting method. Body weight was recorded on day 28 following the treatments. The results of the comparative efficacies of different anthelmintic of ivermectin was 86.75%, followed by levamisole HCl 85.07% and albendazole 92.81%. McMaster fecal egg counting method discloses the percentage of Haemonchus spp. (15.38%), with Trichostrongylus spp, Strongyloides spp., and Cooperia spp. also present. After treatment with ivermectin, levamisole HCl and albendazole, Total Erythrocyte Count (TEC), Hemoglobin (Hb) content and Packed Cell Volume (PCV) were increased significantly (p<0.01 and p>0.05) in goats but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p<0.05 and p>0.01) in all treated goat and body weight was increased significantly (p<0.01) on day 28.International Journal of Natural Sciences (2015), 5(1) 26-34

scholarly journals The influence of physical exertion on basic hematological parameters values and heart rate in trotters

2014 ◽  
Vol 68 (5-6) ◽  
pp. 291-300
Author(s):  
Dajana Slijepcevic ◽  
R. Savic ◽  
Dragisa Trailovic
Keyword(s):  
Heart Rate ◽  
Radio Telemetry ◽  
Hematological Parameters ◽  
Physical Exertion ◽  
Good Health ◽  
Blood Samples ◽  
Optimal Values ◽  
The Moment ◽  
The Relationship ◽  
Hematological Tests

One of very important prerequisites for achieving good results in races, in addition to genetic predisposition, quality training and good health, are optimal values for number of erythrocytes, concentration of haemoglobin and hematocrit, of which depends efficient oxygen supply of muscles during great efforts. The stated values, along with data on heart rate, are useful indicators of the degree of horse fitness and readiness for horse race. The influence of physical exertion on the values of basic hematological parameters as well as on heart rate, was investigated on 6 trotters, in training at the Belgrade racetrack (one head of Italian trotter, male, 3 years old; 3 heads of American trotter, male, 3,4 and 6 years old and two heads of Serbian trotter, female, 4 and 5 years old). The blood samples for hematological tests were taken by punction of jugular vein in resting phase - immediately before the commencement of work, after light trot warming for 3000 m and fast trot for 1000 m, with 30 minutes rest between the two runnings. The heart rate was monitored continuously by radio telemetry cardiometer, from the moment they were taken from their boxes and harnessing to the completion of work. The obtained results confirm the relationship between the rise of heart rate and hematocrit values: maximal hematocrit values were determined after the first running (0.49?0.05, in regard to 0.42?0.03 in resting phase), but 30 minutes after the second running there was a slight drop of hematocrit values (0.46?0.04). The blood samples in both cases were taken after fast trot during which there were recorded maximal pulse values, so in the moment of sampling the pulse lowered close to the values in resting - after the first running from 192.23?19.66, and after the second from 180.33?17.22 to 40.67?5.76.

scholarly journals Effect of putative probiont Enterococcus hirae on the hematological parameters of juvenile African catfish, Clarias gariepinus (Burchell, 1822) during pre- and post-challenge against Aeromonas hydrophila

2018 ◽  
Vol 14 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Nur Hidayahanum Hamid ◽  
Hassan Mohd Daud ◽  
Prapansak Srisapoome ◽  
Hasliza Abu Hassim ◽  
Md Sabri Mohd Yusoff ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Mung Bean ◽  
Hematological Parameters ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Post Challenge ◽  
Control Groups ◽  
Blood Samples ◽  
Mung Bean Sprouts ◽  
Bean Sprouts

Probiotics have been widely known to have the ability to improve the immune system of livestock and aquatic animal. The present study was carried out to evaluate the effect of dietary supplementation of two probiotic isolates of Enterococcus faecium on hematological parameters of juvenile African catfish, Clarias gariepinus during pre- and post-challenge with aquatic pathogen, Aeromonas hydrophila. The probiotics were previously isolated from vegetable wastes (mung bean sprouts, Vigna radiate and cucumber, Cucumis sativus) which have been fermented for 7 days. The experimental fish (270 tails) with an average weight of 5.13 ± 1.03 g were distributed and divided randomly  into  i) control (30 tails), fed with commercial diet ii) E1 (30 tails), fed with  diets  supplemented  with  108 CFU/ml  of  E. faecium isolated from fermented cucumber, iii) E2 (30 tails), fed with 108 CFU/ml  of  E. faecium isolated from fermented mung bean sprouts. The feeding trial was conducted for 50 days. All experimental groups were then challenged with A. hydrophila (1.5 × 106 CFU/mL) via intraperitoneal injection on day 51. Prior to challenge, blood samples were collected from five fish randomly selected from each group on the day 51 (pre-challenge).  After 72 hours of post-challenge, blood samples were again collected from five fish from each groups. The hematological parameters such as total erythrocyte count  (RBC),  total  leucocyte  count  (WBC),  packed cell volume (PCV),  hemoglobin  (Hb), the derived blood indices of mean corpuscular volume (MCV) and corpuscular hemoglobin concentration (MCHC) were examined.  Hematological profiles of pre- and post-challenge infected juvenile catfish were compared with the control groups. The RBC, Hb, WBC, PCV, MCV and MCHC of fish fed with probiotics showed higher significant difference (P<0.05) as compared to control groups during pre- and post-challenge of pathogen. The high level of RBC and WBC during pre- and post-challenge showed the capability of the probiotics to improve the immune response of juvenile African catfish and thus increased the fish disease resistance against A. hydrophila infection. The result suggested that E. faecium could be used effectively as a probiotic in aquaculture.

scholarly journals Study the Oxidative Stress, Some Biochemical and Hematological Parameters in patients with agout at AL-Ramadi city

2016 ◽  
Vol 13 (4) ◽  
pp. 694-701
Author(s):  
Baghdad Science Journal
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
Age Groups ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Blood Picture ◽  
Control Groups ◽  
Blood Samples ◽  
Normal Individuals ◽  
And Control

This study aims to study the effect of gout disease on complete blood picture and biochemical parameters and some non-enzymatic antioxidants, some tracing elements and lipid peroxidation ,in outpatients with gout disease at Al-Ramadi Teaching-Hospital ,Al-Razi Hospital and the study duration from Octo.2013-to May 2014.(50) blood samples were collected from patients with age groups (30-80 years) from both sexes (28 males,22 females),a (30) blood samples (15 males,15 females) were collected from normal individuals as a control group with age groups (27-75 years). Hematological measurement showed no significant differences in size compressed blood cells, the percentages in ( 45.15 +4.99 and 46.87+6.30) % in patient and control groups respectively, hemoglobin concentrations were ( 14.04+1.66 and 14.30+1.93) g/l in patient and control groups respectively, total number of red blood cells ( 5.21+0.43 and 5.12 +0.58) 106/mm3 in patient and control groups respectively with(P?0.05) in ESR (21.06+13.47 and 13.37 +7.45) mm/hr in patient and control groups respectively with (P?0.05), the total number of WBCs were recorded (8.96+2.04 and 7.50+1.69)in patient and control groups respectively. Results showed also significant differences (P?0.05) in uric acid levels (7.42+0.76 and 5.62+0.88) mg/dl,malondialdehyde levels were recorded (4.45+0.64 and 3.21+0.86) in patient and control groups

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